Rh2BG327300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0012548
Physical Location & Seq
Forward (+)
42277930 .. 42289271
11342 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG327300.1

Sequence Viewer

Length: 708 bp
ATGGGTGGGATGGCGAGGAGCTGCGTACAATCAATTCTAAAGTTGGTGAACTCGGTGAATGGGATGGTTGGGCTGGCCATGGTTTTGTACTCGTTGTGGTTGATTAGAGCCTGGCACAAGAGCATGGCTGACTTGCCATTTGAAGACTCTGATTATCCAACAATCCCTTGGTTCATATACATTTTTCTTGGCCTTGGGGCTACTCTGTGTGTGATCACATGTTCAGGACATATTGCTGCAGACACTGCAAATGGTTGTTGCCTTTATTTGTACATGCTGTTTGTCTTTTTACTCCTCATGCTTGAAACTGGAGTGACTGCTGATATATATTTAAACCATGACTGGGAAGAGGACTTTCCTGATGATCCAACTGGGAGATTTGATCAATTCAAAGCATTTGTAAGGTCAAACTTGGACATCTGCAAATGGATTGGCATGTCAGTTATATGCGTACAGGGACTTACATTGTTATTGGCAATGATACTCAAAGCTTTTGGGCTACATCATTATTATGATAGTGATGATGATGATGTTCCTGAGAGGGTTTCACTGCTGAATCACCCCAATCCTTATGTCGTTGGCGACCCTATTTATGGTTCCAAAAATGATGCTTGGCATATAAGGATCAACAGCAAGGCTTATCTCCAATTGCATCATCAACCTCTTGCTGAACCAGAACGTTCTTCATCTTCCTTTGCCAAAGCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tetraspannin

Protein Analysis

235

Amino Acids

26.45

Weight (kDa)

5.2

Isoelectric Point (pI)

44.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tetraspanin PF00335 12 - 115 2.3e-10 Tetraspanin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclI AACGTT 1 cut(s) 679
AclWI GGATC 2 cut(s) 359, 632
AcoI YGGCCR 1 cut(s) 75
AfaI GTAC 4 cut(s) 27, 89, 272, 453
AfiI CCNNNNNNNGG 2 cut(s) 343, 593
AflIII ACRYGT 1 cut(s) 218
AgsI TTSAA 3 cut(s) 143, 305, 391
AjnI CCWGG 1 cut(s) 110
AluBI AGCT 2 cut(s) 21, 491
AluI AGCT 2 cut(s) 21, 491
AlwI GGATC 2 cut(s) 359, 632
AlwNI CAGNNNCTG 1 cut(s) 245
AoxI GGCC 2 cut(s) 75, 190
ApeKI GCWGC 2 cut(s) 21, 236
ArsI GACNNNNNNTTYG 2 cut(s) 122, 154
AsuHPI GGTGA 3 cut(s) 58, 67, 551
BalI TGGCCA 1 cut(s) 77
BbsI GAAGAC 1 cut(s) 150
BbvI GCAGC 2 cut(s) 8, 223
BccI CCATC 2 cut(s) 4, 58
BciT130I CCWGG 1 cut(s) 112
BclI TGATCA 2 cut(s) 213, 382
BfmI CTRYAG 1 cut(s) 237
BisI GCNGC 2 cut(s) 22, 237
BlsI GCNGC 2 cut(s) 23, 238
Bme1390I CCNGG 1 cut(s) 112
BmiI GGNNCC 1 cut(s) 598
BmrFI CCNGG 1 cut(s) 112
BmrI ACTGGG 2 cut(s) 352, 381
BmsI GCATC 2 cut(s) 598, 661
BmuI ACTGGG 2 cut(s) 352, 381
BpiI GAAGAC 1 cut(s) 150
BpmI CTGGAG 1 cut(s) 330
BsaJI CCNNGG 3 cut(s) 78, 167, 193
Bsc4I CCNNNNNNNGG 2 cut(s) 343, 593
Bse1I ACTGG 3 cut(s) 313, 347, 376
Bse3DI GCAATG 1 cut(s) 483
BseBI CCWGG 1 cut(s) 112
BseDI CCNNGG 3 cut(s) 78, 167, 193
BseGI GGATG 2 cut(s) 15, 69
BseLI CCNNNNNNNGG 2 cut(s) 343, 593
BseMI GCAATG 1 cut(s) 483
BseMII CTCAG 1 cut(s) 528
BseNI ACTGG 3 cut(s) 313, 347, 376
BseRI GAGGAG 2 cut(s) 31, 284
BseXI GCAGC 2 cut(s) 8, 223
BshFI GGCC 2 cut(s) 77, 192
BslFI GGGAC 1 cut(s) 471
BslI CCNNNNNNNGG 2 cut(s) 343, 593
BsmFI GGGAC 1 cut(s) 471
BsnI GGCC 2 cut(s) 77, 192
Bsp1407I TGTACA 1 cut(s) 270
Bsp143I GATC 4 cut(s) 213, 364, 382, 624
Bsp19I CCATGG 1 cut(s) 78
BspANI GGCC 2 cut(s) 77, 192
BspCNI CTCAG 1 cut(s) 529
BspLI GGNNCC 1 cut(s) 598
BspMAI CTGCAG 1 cut(s) 241
BspPI GGATC 2 cut(s) 359, 632
BsrDI GCAATG 1 cut(s) 483
BsrGI TGTACA 1 cut(s) 270
BsrI ACTGG 3 cut(s) 313, 347, 376
BssECI CCNNGG 3 cut(s) 78, 167, 193
BssMI GATC 4 cut(s) 213, 364, 382, 624
BssT1I CCWWGG 3 cut(s) 78, 167, 193
Bst2UI CCWGG 1 cut(s) 112
Bst6I CTCTTC 1 cut(s) 342
BstAPI GCANNNNNTGC 1 cut(s) 245
BstAUI TGTACA 1 cut(s) 270
BstC8I GCNNGC 1 cut(s) 75
BstDEI CTNAG 1 cut(s) 537
BstDSI CCRYGG 1 cut(s) 78
BstF5I GGATG 2 cut(s) 15, 69
BstKTI GATC 4 cut(s) 216, 367, 385, 627
BstMBI GATC 4 cut(s) 213, 364, 382, 624
BstMWI GCNNNNNNNGC 1 cut(s) 245
BstNI CCWGG 1 cut(s) 112
BstNSI RCATGY 3 cut(s) 222, 277, 439
BstSCI CCNGG 1 cut(s) 110
BstSFI CTRYAG 1 cut(s) 237
BstV1I GCAGC 2 cut(s) 8, 223
BstV2I GAAGAC 1 cut(s) 150
BsuRI GGCC 2 cut(s) 77, 192
BtgI CCRYGG 1 cut(s) 78
BtsCI GGATG 2 cut(s) 15, 69
BtsI GCAGTG 2 cut(s) 243, 548
BtsIMutI CAGTG 2 cut(s) 243, 548
Cac8I GCNNGC 1 cut(s) 75
CaiI CAGNNNCTG 1 cut(s) 245
Csp6I GTAC 4 cut(s) 26, 88, 271, 452
CviAII CATG 7 cut(s) 79, 124, 219, 274, 298, 338, 436
CviQI GTAC 4 cut(s) 26, 88, 271, 452
DdeI CTNAG 1 cut(s) 537
DpnI GATC 4 cut(s) 215, 366, 384, 626
DpnII GATC 4 cut(s) 213, 364, 382, 624
DraI TTTAAA 1 cut(s) 333
EaeI YGGCCR 1 cut(s) 75
Eam1104I CTCTTC 1 cut(s) 342
EarI CTCTTC 1 cut(s) 342
Eco130I CCWWGG 3 cut(s) 78, 167, 193
EcoRII CCWGG 1 cut(s) 110
EcoT14I CCWWGG 3 cut(s) 78, 167, 193
ErhI CCWWGG 3 cut(s) 78, 167, 193
FaeI CATG 7 cut(s) 82, 127, 222, 277, 301, 341, 439
FaqI GGGAC 1 cut(s) 471
FatI CATG 7 cut(s) 78, 123, 218, 273, 297, 337, 435
FbaI TGATCA 2 cut(s) 213, 382
Fnu4HI GCNGC 2 cut(s) 22, 237
FokI GGATG 2 cut(s) 22, 76
Fsp4HI GCNGC 2 cut(s) 22, 237
GluI GCNGC 2 cut(s) 22, 237
GsuI CTGGAG 1 cut(s) 330
HaeIII GGCC 2 cut(s) 77, 192
Hin1II CATG 7 cut(s) 82, 127, 222, 277, 301, 341, 439
HindIII AAGCTT 1 cut(s) 489
HinfI GANTC 2 cut(s) 146, 556
HphI GGTGA 3 cut(s) 58, 67, 551
Hpy166II GTNNAC 1 cut(s) 49
Hpy188I TCNGA 1 cut(s) 151
Hpy188III TCNNGA 3 cut(s) 225, 359, 536
Hpy8I GTNNAC 1 cut(s) 49
HpyCH4IV ACGT 1 cut(s) 679
HpyCH4V TGCA 4 cut(s) 239, 248, 423, 652
HpyF10VI GCNNNNNNNGC 1 cut(s) 245
HpyF3I CTNAG 1 cut(s) 537
HpySE526I ACGT 1 cut(s) 679
Hsp92II CATG 7 cut(s) 82, 127, 222, 277, 301, 341, 439
Ksp22I TGATCA 2 cut(s) 213, 382
Kzo9I GATC 4 cut(s) 213, 364, 382, 624
LmnI GCTCC 1 cut(s) 18
Lsp1109I GCAGC 2 cut(s) 8, 223
LweI GCATC 2 cut(s) 598, 661
MaeII ACGT 1 cut(s) 679
MaeIII GTNAC 1 cut(s) 313
MalI GATC 4 cut(s) 215, 366, 384, 626
MboI GATC 4 cut(s) 213, 364, 382, 624
MboII GAAGA 4 cut(s) 155, 359, 675, 681
MfeI CAATTG 1 cut(s) 647
MlsI TGGCCA 1 cut(s) 77
MluCI AATT 3 cut(s) 33, 386, 647
MluNI TGGCCA 1 cut(s) 77
MlyI GAGTC 1 cut(s) 140
MmeI TCCRAC 2 cut(s) 182, 392
MnlI CCTC 5 cut(s) 9, 305, 343, 534, 672
Mox20I TGGCCA 1 cut(s) 77
MscI TGGCCA 1 cut(s) 77
MseI TTAA 1 cut(s) 332
MslI CAYNNNNRTG 1 cut(s) 510
Msp20I TGGCCA 1 cut(s) 77
MspR9I CCNGG 1 cut(s) 112
MunI CAATTG 1 cut(s) 647
MvaI CCWGG 1 cut(s) 112
MwoI GCNNNNNNNGC 1 cut(s) 245
NcoI CCATGG 1 cut(s) 78
NdeII GATC 4 cut(s) 213, 364, 382, 624
NlaIII CATG 7 cut(s) 82, 127, 222, 277, 301, 341, 439
NlaIV GGNNCC 1 cut(s) 598
NmuCI GTSAC 1 cut(s) 313
NspI RCATGY 3 cut(s) 222, 277, 439
PciI ACATGT 1 cut(s) 218
PfeI GAWTC 1 cut(s) 556
PkrI GCNGC 2 cut(s) 23, 238
PleI GAGTC 1 cut(s) 140
PpsI GAGTC 1 cut(s) 140
PscI ACATGT 1 cut(s) 218
Psp1406I AACGTT 1 cut(s) 679
Psp6I CCWGG 1 cut(s) 110
PspGI CCWGG 1 cut(s) 110
PspN4I GGNNCC 1 cut(s) 598
PstI CTGCAG 1 cut(s) 241
PstNI CAGNNNCTG 1 cut(s) 245
RsaI GTAC 4 cut(s) 27, 89, 272, 453
RsaNI GTAC 4 cut(s) 26, 88, 271, 452
RseI CAYNNNNRTG 1 cut(s) 510
SaqAI TTAA 1 cut(s) 332
SatI GCNGC 2 cut(s) 22, 237
Sau3AI GATC 4 cut(s) 213, 364, 382, 624
SchI GAGTC 1 cut(s) 140
ScrFI CCNGG 1 cut(s) 112
SetI ASST 5 cut(s) 23, 407, 493, 664, 682
SfaNI GCATC 2 cut(s) 598, 661
SfcI CTRYAG 1 cut(s) 237
SmiMI CAYNNNNRTG 1 cut(s) 510
Sse9I AATT 3 cut(s) 33, 386, 647
StyD4I CCNGG 1 cut(s) 110
StyI CCWWGG 3 cut(s) 78, 167, 193
TaiI ACGT 1 cut(s) 682
TasI AATT 3 cut(s) 33, 386, 647
TatI WGTACW 2 cut(s) 87, 270
TfiI GAWTC 1 cut(s) 556
Tru1I TTAA 1 cut(s) 332
Tru9I TTAA 1 cut(s) 332
TscAI CASTG 2 cut(s) 250, 555
TseFI GTSAC 1 cut(s) 313
TseI GCWGC 2 cut(s) 21, 236
Tsp45I GTSAC 1 cut(s) 313
TspDTI ATGAA 2 cut(s) 163, 675
TspRI CASTG 2 cut(s) 250, 555
XceI RCATGY 3 cut(s) 222, 277, 439
XcmI CCANNNNNNNNNTGG 1 cut(s) 165
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.