Rw2G025830

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0012548
Physical Location & Seq
Forward (+)
39244293 .. 39248954
4662 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G025830.1

Sequence Viewer

Length: 489 bp
ATGAACTGCAGGTTCATATACATTTTTCTTGGCCTTGGGGTTACTCTGTGTGTGATCACATGTTCAGGACATATTGCTGCAGACACTGCAAATGGTTGTTGCCTTTATTTGTACATGCTGTTTGTCTTTTTACTCCTCATGCTTGAAACTGGAGTGACTGCTGATATATATTTAAACCATGACTGGGAAGAGGACTTTCCTGATGATCCAACTGGGAGATTTGATCAATTCAAAGCATTTGTAAGGTCAAACTTGGACATCTGCAAATGGATTGGCATGTCAGTTATATGCGTACAGGGACTTACATTGTTATTGGCAATGATACTCAAAGCTTTTGGGCCACATCATTATTATGATAGTGATGATGATAATGTTCCTGAGAGGGTTTCACTTCTGAATCACCCCAATCCTTATGTCGTTGGCGACCCTATTTATGGTTCCAAAAATGATGCTTGGCATATAAGGATCAACAGCAAGACAAACAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tetraspannin

Protein Analysis

162

Amino Acids

18.4

Weight (kDa)

5.14

Isoelectric Point (pI)

29.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tetraspanin PF00335 4 - 62 4e-06 Tetraspanin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 200, 473
AfaI GTAC 2 cut(s) 113, 294
AfiI CCNNNNNNNGG 2 cut(s) 184, 434
AflIII ACRYGT 1 cut(s) 59
AgsI TTSAA 2 cut(s) 146, 232
AluBI AGCT 1 cut(s) 332
AluI AGCT 1 cut(s) 332
AlwI GGATC 2 cut(s) 200, 473
AlwNI CAGNNNCTG 1 cut(s) 86
AoxI GGCC 2 cut(s) 31, 338
ApeKI GCWGC 1 cut(s) 77
AspS9I GGNCC 1 cut(s) 338
AsuHPI GGTGA 1 cut(s) 392
BbvI GCAGC 1 cut(s) 64
BclI TGATCA 2 cut(s) 54, 223
BfmI CTRYAG 2 cut(s) 7, 78
BisI GCNGC 1 cut(s) 78
BlsI GCNGC 1 cut(s) 79
BmgT120I GGNCC 1 cut(s) 338
BmiI GGNNCC 1 cut(s) 439
BmrI ACTGGG 2 cut(s) 193, 222
BmsI GCATC 1 cut(s) 439
BmuI ACTGGG 2 cut(s) 193, 222
BpmI CTGGAG 1 cut(s) 171
BsaJI CCNNGG 1 cut(s) 34
Bsc4I CCNNNNNNNGG 2 cut(s) 184, 434
Bse1I ACTGG 3 cut(s) 154, 188, 217
Bse3DI GCAATG 1 cut(s) 324
BseDI CCNNGG 1 cut(s) 34
BseLI CCNNNNNNNGG 2 cut(s) 184, 434
BseMI GCAATG 1 cut(s) 324
BseMII CTCAG 1 cut(s) 369
BseNI ACTGG 3 cut(s) 154, 188, 217
BseRI GAGGAG 1 cut(s) 125
BseXI GCAGC 1 cut(s) 64
BshFI GGCC 2 cut(s) 33, 340
BslFI GGGAC 1 cut(s) 312
BslI CCNNNNNNNGG 2 cut(s) 184, 434
BsmFI GGGAC 1 cut(s) 312
BsnI GGCC 2 cut(s) 33, 340
Bsp1407I TGTACA 1 cut(s) 111
Bsp143I GATC 4 cut(s) 54, 205, 223, 465
BspANI GGCC 2 cut(s) 33, 340
BspCNI CTCAG 1 cut(s) 370
BspLI GGNNCC 1 cut(s) 439
BspMAI CTGCAG 2 cut(s) 11, 82
BspPI GGATC 2 cut(s) 200, 473
BsrDI GCAATG 1 cut(s) 324
BsrGI TGTACA 1 cut(s) 111
BsrI ACTGG 3 cut(s) 154, 188, 217
BssECI CCNNGG 1 cut(s) 34
BssMI GATC 4 cut(s) 54, 205, 223, 465
BssT1I CCWWGG 1 cut(s) 34
Bst6I CTCTTC 1 cut(s) 183
BstAPI GCANNNNNTGC 1 cut(s) 86
BstAUI TGTACA 1 cut(s) 111
BstDEI CTNAG 1 cut(s) 378
BstKTI GATC 4 cut(s) 57, 208, 226, 468
BstMBI GATC 4 cut(s) 54, 205, 223, 465
BstMWI GCNNNNNNNGC 1 cut(s) 86
BstNSI RCATGY 3 cut(s) 63, 118, 280
BstSFI CTRYAG 2 cut(s) 7, 78
BstV1I GCAGC 1 cut(s) 64
BsuRI GGCC 2 cut(s) 33, 340
BtsI GCAGTG 1 cut(s) 84
BtsIMutI CAGTG 1 cut(s) 84
CaiI CAGNNNCTG 1 cut(s) 86
Cfr13I GGNCC 1 cut(s) 338
Csp6I GTAC 2 cut(s) 112, 293
CviAII CATG 5 cut(s) 60, 115, 139, 179, 277
CviJI RGCY 3 cut(s) 33, 332, 340
CviKI_1 RGCY 3 cut(s) 33, 332, 340
CviQI GTAC 2 cut(s) 112, 293
DdeI CTNAG 1 cut(s) 378
DpnI GATC 4 cut(s) 56, 207, 225, 467
DpnII GATC 4 cut(s) 54, 205, 223, 465
DraI TTTAAA 1 cut(s) 174
Eam1104I CTCTTC 1 cut(s) 183
EarI CTCTTC 1 cut(s) 183
Eco130I CCWWGG 1 cut(s) 34
EcoT14I CCWWGG 1 cut(s) 34
ErhI CCWWGG 1 cut(s) 34
FaeI CATG 5 cut(s) 63, 118, 142, 182, 280
FaqI GGGAC 1 cut(s) 312
FatI CATG 5 cut(s) 59, 114, 138, 178, 276
FbaI TGATCA 2 cut(s) 54, 223
Fnu4HI GCNGC 1 cut(s) 78
Fsp4HI GCNGC 1 cut(s) 78
GluI GCNGC 1 cut(s) 78
GsuI CTGGAG 1 cut(s) 171
HaeIII GGCC 2 cut(s) 33, 340
Hin1II CATG 5 cut(s) 63, 118, 142, 182, 280
HindIII AAGCTT 1 cut(s) 330
HinfI GANTC 1 cut(s) 397
HphI GGTGA 1 cut(s) 392
Hpy188I TCNGA 1 cut(s) 396
Hpy188III TCNNGA 3 cut(s) 66, 200, 377
HpyCH4V TGCA 4 cut(s) 9, 80, 89, 264
HpyF10VI GCNNNNNNNGC 1 cut(s) 86
HpyF3I CTNAG 1 cut(s) 378
Hsp92II CATG 5 cut(s) 63, 118, 142, 182, 280
Ksp22I TGATCA 2 cut(s) 54, 223
Kzo9I GATC 4 cut(s) 54, 205, 223, 465
LpnPI CCDG 8 cut(s) 51, 135, 169, 198, 213, 281, 390, 469
Lsp1109I GCAGC 1 cut(s) 64
LweI GCATC 1 cut(s) 439
MaeIII GTNAC 2 cut(s) 40, 154
MalI GATC 4 cut(s) 56, 207, 225, 467
MboI GATC 4 cut(s) 54, 205, 223, 465
MboII GAAGA 1 cut(s) 200
MluCI AATT 1 cut(s) 227
MmeI TCCRAC 1 cut(s) 233
MnlI CCTC 3 cut(s) 146, 184, 375
MseI TTAA 1 cut(s) 173
MslI CAYNNNNRTG 1 cut(s) 351
MwoI GCNNNNNNNGC 1 cut(s) 86
NdeII GATC 4 cut(s) 54, 205, 223, 465
NlaIII CATG 5 cut(s) 63, 118, 142, 182, 280
NlaIV GGNNCC 1 cut(s) 439
NmuCI GTSAC 1 cut(s) 154
NspI RCATGY 3 cut(s) 63, 118, 280
PciI ACATGT 1 cut(s) 59
PfeI GAWTC 1 cut(s) 397
PkrI GCNGC 1 cut(s) 79
PscI ACATGT 1 cut(s) 59
PspN4I GGNNCC 1 cut(s) 439
PspPI GGNCC 1 cut(s) 338
PstI CTGCAG 2 cut(s) 11, 82
PstNI CAGNNNCTG 1 cut(s) 86
RsaI GTAC 2 cut(s) 113, 294
RsaNI GTAC 2 cut(s) 112, 293
RseI CAYNNNNRTG 1 cut(s) 351
SaqAI TTAA 1 cut(s) 173
SatI GCNGC 1 cut(s) 78
Sau3AI GATC 4 cut(s) 54, 205, 223, 465
Sau96I GGNCC 1 cut(s) 338
SetI ASST 4 cut(s) 14, 248, 334, 488
SfaNI GCATC 1 cut(s) 439
SfcI CTRYAG 2 cut(s) 7, 78
SmiMI CAYNNNNRTG 1 cut(s) 351
Sse9I AATT 1 cut(s) 227
StyI CCWWGG 1 cut(s) 34
TasI AATT 1 cut(s) 227
TatI WGTACW 1 cut(s) 111
TfiI GAWTC 1 cut(s) 397
Tru1I TTAA 1 cut(s) 173
Tru9I TTAA 1 cut(s) 173
TscAI CASTG 1 cut(s) 91
TseFI GTSAC 1 cut(s) 154
TseI GCWGC 1 cut(s) 77
Tsp45I GTSAC 1 cut(s) 154
TspDTI ATGAA 2 cut(s) 4, 17
TspRI CASTG 1 cut(s) 91
XceI RCATGY 3 cut(s) 63, 118, 280
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.