MD17G1280800.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0013598
Physical Location & Seq
Forward (+)
34068971 .. 34071214
2244 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1280800.v1.1.491

Sequence Viewer

Length: 888 bp
ATGTGGAGTGAAGGAGAAGTACCCGTCCTCGGGTACTGGCTTCAATGGCAAGTCCTGGTTTGCGCTCTGATTATAGCAGCTCCGGCCACCGTAGCTCTCAAATTCGCATGGAAAGGAAAGCCGGAGCCGCCACTGAAATCGGTGGAGCTGTGGGGCACTTGCTGGAGAACCCTCGATCCTCTCTGGCTTCTCTTCTATCGAGCTTTCGCCTTTGTGTTAATGGCCAAACTGCTCTTCGACATGACCTCCCAATTTGGTCTTTTCACGCTCGTTTTCTACACCCAGTGGACCTTTGCTTTGGTTATGCTCTATTTCGGGCTGGGGACAATTATATCTGCGCATGGATGTTGGGTTGGAAGGTCTTCTTACACAGATGATCAAATCAAGGGTAAAGTGAAACCGAAAAGCAACCAAGCTCGAAATGCGATTCAACAAAGAGCTGGATTCTTTGGAAATCTGATGCTCATCATTTATCAGACGTGCGCAGGTGCTGTAATGCTAACAGACATTGTATTTTGGTGTCTGATACTGCCATTCCTCACAAGTATGGAATTCCAACTAACCTTTTTGATAGGTGCCATACATGCTCTCAACGCTGTTTTTCTAATCGTGGACACTGCTCTCAATAGGCTCCCATTTTCGAAGTTGGGTTTTGCATATTTTGCGCTCTTCGGAGTCCTCTATATTAGTTTTCAATGGCTCATCCACGCCTTTGGTGTTAAATGGTGGCCATATCCTTTCCTTGAGCTGAACACACCATTGGCACCTTTGTGGTATTTTGGGTTGGCAGTGTGGCACATCCCCTGTTACTGGATTTACTCCCTCATCATCAACTCTAAAGTTTCAATCCTCCCCAGATTGTTTCCTCGTTCATTTCTACGATCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

296

Amino Acids

33.94

Weight (kDa)

9.3

Isoelectric Point (pI)

29.96

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 476
Acc16I TGCGCA 2 cut(s) 339, 484
Acc36I ACCTGC 1 cut(s) 476
AccB1I GGYRCC 2 cut(s) 575, 763
AciI CCGC 1 cut(s) 128
AclWI GGATC 2 cut(s) 170, 876
AcoI YGGCCR 3 cut(s) 84, 222, 728
AcsI RAATTY 2 cut(s) 101, 551
AdeI CACNNNGTG 1 cut(s) 285
AfaI GTAC 2 cut(s) 21, 35
AfiI CCNNNNNNNGG 3 cut(s) 29, 30, 810
AgsI TTSAA 4 cut(s) 44, 431, 695, 846
AjiI CACGTC 1 cut(s) 480
AjnI CCWGG 1 cut(s) 54
AjuI GAANNNNNNNTTGG 4 cut(s) 218, 250, 743, 775
AleI CACNNNNGTG 1 cut(s) 769
AloI GAACNNNNNNTCC 2 cut(s) 160, 192
AluBI AGCT 7 cut(s) 80, 95, 148, 203, 416, 440, 748
AluI AGCT 7 cut(s) 80, 95, 148, 203, 416, 440, 748
AlwI GGATC 2 cut(s) 170, 876
AlwNI CAGNNNCTG 1 cut(s) 491
Ama87I CYCGRG 1 cut(s) 29
AoxI GGCC 3 cut(s) 84, 222, 728
ApeKI GCWGC 1 cut(s) 77
ApoI RAATTY 2 cut(s) 101, 551
ArsI GACNNNNNNTTYG 2 cut(s) 280, 312
Asp700I GAANNNNTTC 1 cut(s) 361
AspLEI GCGC 4 cut(s) 65, 340, 485, 667
AspS9I GGNCC 1 cut(s) 288
AsuII TTCGAA 1 cut(s) 641
AvaI CYCGRG 1 cut(s) 29
AvaII GGWCC 1 cut(s) 288
BaeGI GKGCMC 1 cut(s) 158
BalI TGGCCA 2 cut(s) 224, 730
BanI GGYRCC 2 cut(s) 575, 763
BarI GAAGNNNNNNTAC 1 cut(s) 35
BbsI GAAGAC 1 cut(s) 354
BbvI GCAGC 1 cut(s) 89
BciT130I CCWGG 1 cut(s) 56
BclI TGATCA 1 cut(s) 376
BfuAI ACCTGC 1 cut(s) 476
BisI GCNGC 2 cut(s) 78, 128
BlsI GCNGC 2 cut(s) 79, 129
Bme1390I CCNGG 1 cut(s) 56
Bme18I GGWCC 1 cut(s) 288
BmeT110I CYCGRG 1 cut(s) 29
BmgBI CACGTC 1 cut(s) 480
BmgT120I GGNCC 1 cut(s) 288
BmiI GGNNCC 4 cut(s) 126, 577, 632, 765
BmrFI CCNGG 1 cut(s) 56
BmrI ACTGGG 1 cut(s) 277
BmsI GCATC 1 cut(s) 450
BmuI ACTGGG 1 cut(s) 277
BpiI GAAGAC 1 cut(s) 354
BpmI CTGGAG 1 cut(s) 184
Bpu14I TTCGAA 1 cut(s) 641
BpuEI CTTGAG 1 cut(s) 764
BsaBI GATNNNNATC 1 cut(s) 464
BsaJI CCNNGG 1 cut(s) 28
BsaXI ACNNNNNCTCC 2 cut(s) 230, 260
Bsc4I CCNNNNNNNGG 3 cut(s) 29, 30, 810
Bse1I ACTGG 3 cut(s) 41, 283, 815
Bse8I GATNNNNATC 1 cut(s) 464
BseBI CCWGG 1 cut(s) 56
BseDI CCNNGG 1 cut(s) 28
BseGI GGATG 3 cut(s) 350, 702, 798
BseJI GATNNNNATC 1 cut(s) 464
BseLI CCNNNNNNNGG 3 cut(s) 29, 30, 810
BseNI ACTGG 3 cut(s) 41, 283, 815
BseSI GKGCMC 1 cut(s) 158
BseXI GCAGC 1 cut(s) 89
BseYI CCCAGC 1 cut(s) 319
BshFI GGCC 3 cut(s) 86, 224, 730
BshNI GGYRCC 2 cut(s) 575, 763
BsiHKCI CYCGRG 1 cut(s) 29
BsiSI CCGG 2 cut(s) 83, 122
BslFI GGGAC 1 cut(s) 337
BslI CCNNNNNNNGG 3 cut(s) 29, 30, 810
BsmFI GGGAC 1 cut(s) 337
BsnI GGCC 3 cut(s) 86, 224, 730
BsoBI CYCGRG 1 cut(s) 29
Bsp119I TTCGAA 1 cut(s) 641
Bsp1286I GDGCHC 1 cut(s) 158
Bsp143I GATC 3 cut(s) 175, 376, 881
BspACI CCGC 1 cut(s) 128
BspANI GGCC 3 cut(s) 86, 224, 730
BspLI GGNNCC 4 cut(s) 126, 577, 632, 765
BspMI ACCTGC 1 cut(s) 476
BspPI GGATC 2 cut(s) 170, 876
BspQI GCTCTTC 2 cut(s) 239, 674
BspT104I TTCGAA 1 cut(s) 641
BspT107I GGYRCC 2 cut(s) 575, 763
BsrI ACTGG 3 cut(s) 41, 283, 815
BssECI CCNNGG 1 cut(s) 28
BssMI GATC 3 cut(s) 175, 376, 881
Bst2UI CCWGG 1 cut(s) 56
Bst4CI ACNGT 1 cut(s) 91
Bst6I CTCTTC 3 cut(s) 197, 239, 674
BstAPI GCANNNNNTGC 1 cut(s) 662
BstBI TTCGAA 1 cut(s) 641
BstF5I GGATG 3 cut(s) 350, 702, 798
BstHHI GCGC 4 cut(s) 65, 340, 485, 667
BstKTI GATC 3 cut(s) 178, 379, 884
BstMBI GATC 3 cut(s) 175, 376, 881
BstMWI GCNNNNNNNGC 8 cut(s) 46, 83, 92, 127, 422, 584, 593, 662
BstNI CCWGG 1 cut(s) 56
BstNSI RCATGY 1 cut(s) 587
BstSCI CCNGG 1 cut(s) 54
BstSLI GKGCMC 1 cut(s) 158
BstV1I GCAGC 1 cut(s) 89
BstV2I GAAGAC 1 cut(s) 354
BstXI CCANNNNNNTGG 1 cut(s) 713
BsuRI GGCC 3 cut(s) 86, 224, 730
BtrI CACGTC 1 cut(s) 480
BtsCI GGATG 3 cut(s) 350, 702, 798
BtsI GCAGTG 2 cut(s) 615, 795
BtsIMutI CAGTG 4 cut(s) 131, 290, 615, 795
BveI ACCTGC 1 cut(s) 476
CaiI CAGNNNCTG 1 cut(s) 491
CfoI GCGC 4 cut(s) 65, 340, 485, 667
Cfr13I GGNCC 1 cut(s) 288
Csp6I GTAC 2 cut(s) 20, 34
CviAII CATG 4 cut(s) 108, 241, 341, 584
CviQI GTAC 2 cut(s) 20, 34
DpnI GATC 3 cut(s) 177, 378, 883
DpnII GATC 3 cut(s) 175, 376, 881
DraIII CACNNNGTG 1 cut(s) 285
EaeI YGGCCR 3 cut(s) 84, 222, 728
Eam1104I CTCTTC 3 cut(s) 197, 239, 674
EarI CTCTTC 3 cut(s) 197, 239, 674
Eco47I GGWCC 1 cut(s) 288
Eco88I CYCGRG 1 cut(s) 29
EcoRI GAATTC 1 cut(s) 551
EcoRII CCWGG 1 cut(s) 54
FaeI CATG 4 cut(s) 111, 244, 344, 587
FalI AAGNNNNNCTT 2 cut(s) 349, 381
FaqI GGGAC 1 cut(s) 337
FatI CATG 4 cut(s) 107, 240, 340, 583
FbaI TGATCA 1 cut(s) 376
Fnu4HI GCNGC 2 cut(s) 78, 128
FokI GGATG 3 cut(s) 357, 689, 785
Fsp4HI GCNGC 2 cut(s) 78, 128
FspI TGCGCA 2 cut(s) 339, 484
GlaI GCGC 4 cut(s) 64, 339, 484, 666
GluI GCNGC 2 cut(s) 78, 128
GsaI CCCAGC 1 cut(s) 323
GsuI CTGGAG 1 cut(s) 184
HaeIII GGCC 3 cut(s) 86, 224, 730
HapII CCGG 2 cut(s) 83, 122
HhaI GCGC 4 cut(s) 65, 340, 485, 667
Hin1II CATG 4 cut(s) 111, 244, 344, 587
Hin6I GCGC 4 cut(s) 63, 338, 483, 665
HinP1I GCGC 4 cut(s) 63, 338, 483, 665
HinfI GANTC 3 cut(s) 427, 444, 675
HpaII CCGG 2 cut(s) 83, 122
Hpy166II GTNNAC 2 cut(s) 288, 613
Hpy188I TCNGA 5 cut(s) 69, 459, 477, 525, 674
Hpy8I GTNNAC 2 cut(s) 288, 613
HpyAV CCTTC 2 cut(s) 5, 351
HpyCH4III ACNGT 1 cut(s) 91
HpyCH4IV ACGT 1 cut(s) 479
HpyCH4V TGCA 1 cut(s) 656
HpyF10VI GCNNNNNNNGC 8 cut(s) 46, 83, 92, 127, 422, 584, 593, 662
HpySE526I ACGT 1 cut(s) 479
Hsp92II CATG 4 cut(s) 111, 244, 344, 587
HspAI GCGC 4 cut(s) 63, 338, 483, 665
Ksp22I TGATCA 1 cut(s) 376
Kzo9I GATC 3 cut(s) 175, 376, 881
LguI GCTCTTC 2 cut(s) 239, 674
LmnI GCTCC 4 cut(s) 85, 124, 145, 636
Lsp1109I GCAGC 1 cut(s) 89
LweI GCATC 1 cut(s) 450
MaeII ACGT 1 cut(s) 479
MaeIII GTNAC 1 cut(s) 806
MalI GATC 3 cut(s) 177, 378, 883
MboI GATC 3 cut(s) 175, 376, 881
MboII GAAGA 4 cut(s) 184, 226, 354, 661
MhlI GDGCHC 1 cut(s) 158
MlsI TGGCCA 2 cut(s) 224, 730
MluCI AATT 4 cut(s) 101, 251, 327, 551
MluNI TGGCCA 2 cut(s) 224, 730
MlyI GAGTC 1 cut(s) 684
MmeI TCCRAC 2 cut(s) 334, 580
MnlI CCTC 9 cut(s) 38, 182, 189, 256, 548, 689, 833, 860, 876
Mox20I TGGCCA 2 cut(s) 224, 730
MroXI GAANNNNTTC 1 cut(s) 361
MscI TGGCCA 2 cut(s) 224, 730
MseI TTAA 2 cut(s) 218, 720
MslI CAYNNNNRTG 2 cut(s) 545, 769
Msp20I TGGCCA 2 cut(s) 224, 730
MspI CCGG 2 cut(s) 83, 122
MspR9I CCNGG 1 cut(s) 56
MvaI CCWGG 1 cut(s) 56
MwoI GCNNNNNNNGC 8 cut(s) 46, 83, 92, 127, 422, 584, 593, 662
NdeII GATC 3 cut(s) 175, 376, 881
NlaIII CATG 4 cut(s) 111, 244, 344, 587
NlaIV GGNNCC 4 cut(s) 126, 577, 632, 765
NsbI TGCGCA 2 cut(s) 339, 484
NspI RCATGY 1 cut(s) 587
NspV TTCGAA 1 cut(s) 641
OliI CACNNNNGTG 1 cut(s) 769
PaqCI CACCTGC 1 cut(s) 476
PciSI GCTCTTC 2 cut(s) 239, 674
PdmI GAANNNNTTC 1 cut(s) 361
PfeI GAWTC 2 cut(s) 427, 444
PkrI GCNGC 2 cut(s) 79, 129
PleI GAGTC 1 cut(s) 683
PpsI GAGTC 1 cut(s) 683
Psp6I CCWGG 1 cut(s) 54
PspFI CCCAGC 1 cut(s) 319
PspGI CCWGG 1 cut(s) 54
PspN4I GGNNCC 4 cut(s) 126, 577, 632, 765
PspPI GGNCC 1 cut(s) 288
PstNI CAGNNNCTG 1 cut(s) 491
RsaI GTAC 2 cut(s) 21, 35
RsaNI GTAC 2 cut(s) 20, 34
RseI CAYNNNNRTG 2 cut(s) 545, 769
SapI GCTCTTC 2 cut(s) 239, 674
SaqAI TTAA 2 cut(s) 218, 720
SatI GCNGC 2 cut(s) 78, 128
Sau3AI GATC 3 cut(s) 175, 376, 881
Sau96I GGNCC 1 cut(s) 288
SchI GAGTC 1 cut(s) 684
ScrFI CCNGG 1 cut(s) 56
SduI GDGCHC 1 cut(s) 158
SfaNI GCATC 1 cut(s) 450
SfuI TTCGAA 1 cut(s) 641
SinI GGWCC 1 cut(s) 288
SmiMI CAYNNNNRTG 2 cut(s) 545, 769
SmlI CTYRAG 1 cut(s) 743
SmoI CTYRAG 1 cut(s) 743
Sse9I AATT 4 cut(s) 101, 251, 327, 551
SsiI CCGC 1 cut(s) 128
StyD4I CCNGG 1 cut(s) 54
TaaI ACNGT 1 cut(s) 91
TaiI ACGT 1 cut(s) 482
TaqI TCGA 5 cut(s) 174, 199, 237, 418, 641
TasI AATT 4 cut(s) 101, 251, 327, 551
TauI GCSGC 1 cut(s) 130
TfiI GAWTC 2 cut(s) 427, 444
Tru1I TTAA 2 cut(s) 218, 720
Tru9I TTAA 2 cut(s) 218, 720
TscAI CASTG 4 cut(s) 138, 290, 622, 795
TseI GCWGC 1 cut(s) 77
TspDTI ATGAA 1 cut(s) 861
TspRI CASTG 4 cut(s) 138, 290, 622, 795
VpaK11BI GGWCC 1 cut(s) 288
XapI RAATTY 2 cut(s) 101, 551
XceI RCATGY 1 cut(s) 587
XmnI GAANNNNTTC 1 cut(s) 361
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.