Rorug03G0225500

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0013598
Physical Location & Seq
Forward (+)
20707089 .. 20707871
783 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0225500.1

Sequence Viewer

Length: 783 bp
ATGGCGAAGAAAAGGCAAAGACAGCTGGTGGAAAATCCCCCGCACAAGAAAAGTACTCCGAGTAGTGGTCTTATTGACGCTTCAAACTCAGACAACGATCTGGATCTGCAGGATAGTTGGGTGATTGTCAAAAAGCAGAGAGTTAACATTTTAGTACCTGGACTTCCTGTTGCTAATAAATCACCACTCCCAAGCCCAGAACCAAGTCAGCTGCAACTCGTGGCTAGAGAAACAGTAGAAAGTGGACCACAGCTTCCTGCCGATACACATCCCAAAACGACTTTGGTTCATGAGAAAAAGAAGATTAAACCTGTTGCCCCTAAAAGGGCTGTGCAGCTTGCTAAGAAAGCTTCTCCTGCTGCTGAATACGTTCCAACCGTTTCCCAGTCAATGAGGCGAGAATTAAGCACAAAATCACAAACCCCAGATCAGATGGCTACTTCACAGTATCAGAGAGCTCTAGGAGTATCTATGACCTCGAAAGCCATCATGCAGCGAAGAAGATTACAACATGTTTTCCTGGATCAGGGAATGTTGCTGAATGAAAGGCTGAGAGCTCGGAATATTGAGAGGAAGATTCAGAATGCTGGTGGGTTAAGCAGGTGGTTAGCATCATTGGGACTGGAGCAGTTTGTTAGAATTTTTCAGAGGAAAGGTTTCAGTAAATTTCAGTTGGTGAATTTGAACATGAAAAAGCTCAAAGATATGGGTGCAAATGCAGTTGGGCCAAGAAGGAAATTGATGCATGCAATAGACTGCTTTTGCCAACCATCCTATTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

260

Amino Acids

29.27

Weight (kDa)

10.44

Isoelectric Point (pI)

63.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SAM_2 PF07647 198 - 253 1.3e-14 SAM domain (Sterile alpha motif)
SAM_1 PF00536 199 - 253 2.9e-09 SAM domain (Sterile alpha motif)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 591
Acc36I ACCTGC 1 cut(s) 591
AciI CCGC 1 cut(s) 41
AclWI GGATC 2 cut(s) 111, 531
AcsI RAATTY 3 cut(s) 639, 665, 679
AfaI GTAC 2 cut(s) 55, 156
AfiI CCNNNNNNNGG 4 cut(s) 65, 324, 325, 526
AflIII ACRYGT 1 cut(s) 511
AgsI TTSAA 2 cut(s) 84, 685
AjnI CCWGG 2 cut(s) 157, 519
AluBI AGCT 8 cut(s) 25, 211, 253, 337, 350, 458, 557, 697
AluI AGCT 8 cut(s) 25, 211, 253, 337, 350, 458, 557, 697
Alw21I GWGCWC 2 cut(s) 460, 559
AlwI GGATC 2 cut(s) 111, 531
AoxI GGCC 1 cut(s) 725
ApeKI GCWGC 4 cut(s) 211, 334, 359, 493
ApoI RAATTY 3 cut(s) 639, 665, 679
Asp700I GAANNNNTTC 2 cut(s) 369, 656
AspS9I GGNCC 2 cut(s) 245, 725
AsuHPI GGTGA 3 cut(s) 133, 174, 688
AvaII GGWCC 1 cut(s) 245
BanII GRGCYC 2 cut(s) 460, 559
BarI GAAGNNNNNNTAC 2 cut(s) 147, 179
BauI CACGAG 1 cut(s) 218
Bbv12I GWGCWC 2 cut(s) 460, 559
BbvI GCAGC 4 cut(s) 198, 346, 346, 505
BccI CCATC 3 cut(s) 427, 494, 778
BciT130I CCWGG 2 cut(s) 159, 521
BfaI CTAG 2 cut(s) 225, 461
BfmI CTRYAG 1 cut(s) 107
BfuAI ACCTGC 1 cut(s) 591
BisI GCNGC 4 cut(s) 212, 335, 360, 494
BlsI GCNGC 4 cut(s) 213, 336, 361, 495
BmcAI AGTACT 1 cut(s) 55
Bme1390I CCNGG 2 cut(s) 159, 521
Bme18I GGWCC 1 cut(s) 245
BmgT120I GGNCC 2 cut(s) 245, 725
BmrFI CCNGG 2 cut(s) 159, 521
BmrI ACTGGG 1 cut(s) 379
BmsI GCATC 2 cut(s) 620, 732
BmuI ACTGGG 1 cut(s) 379
BpmI CTGGAG 1 cut(s) 644
BsaBI GATNNNNATC 2 cut(s) 102, 267
Bsc4I CCNNNNNNNGG 4 cut(s) 65, 324, 325, 526
Bse1I ACTGG 2 cut(s) 385, 627
Bse8I GATNNNNATC 2 cut(s) 102, 267
BseBI CCWGG 2 cut(s) 159, 521
BseGI GGATG 2 cut(s) 268, 770
BseJI GATNNNNATC 2 cut(s) 102, 267
BseLI CCNNNNNNNGG 4 cut(s) 65, 324, 325, 526
BseMII CTCAG 2 cut(s) 102, 542
BseNI ACTGG 2 cut(s) 385, 627
BseXI GCAGC 4 cut(s) 198, 346, 346, 505
BsgI GTGCAG 1 cut(s) 353
BshFI GGCC 1 cut(s) 727
BsiHKAI GWGCWC 2 cut(s) 460, 559
BslFI GGGAC 1 cut(s) 633
BslI CCNNNNNNNGG 4 cut(s) 65, 324, 325, 526
BsmFI GGGAC 1 cut(s) 633
BsmI GAATGC 1 cut(s) 589
BsnI GGCC 1 cut(s) 727
Bsp1286I GDGCHC 2 cut(s) 460, 559
Bsp143I GATC 4 cut(s) 97, 103, 427, 523
BspACI CCGC 1 cut(s) 41
BspANI GGCC 1 cut(s) 727
BspCNI CTCAG 2 cut(s) 101, 543
BspHI TCATGA 1 cut(s) 289
BspMAI CTGCAG 1 cut(s) 111
BspMI ACCTGC 1 cut(s) 591
BspPI GGATC 2 cut(s) 111, 531
BsrI ACTGG 2 cut(s) 385, 627
BssMI GATC 4 cut(s) 97, 103, 427, 523
BssSI CACGAG 1 cut(s) 218
Bst2BI CACGAG 1 cut(s) 218
Bst2UI CCWGG 2 cut(s) 159, 521
Bst4CI ACNGT 3 cut(s) 235, 379, 447
BstC8I GCNNGC 2 cut(s) 339, 747
BstDEI CTNAG 3 cut(s) 88, 342, 551
BstENI CCTNNNNNAGG 1 cut(s) 524
BstF5I GGATG 2 cut(s) 268, 770
BstKTI GATC 4 cut(s) 100, 106, 430, 526
BstMBI GATC 4 cut(s) 97, 103, 427, 523
BstMWI GCNNNNNNNGC 3 cut(s) 22, 347, 356
BstNI CCWGG 2 cut(s) 159, 521
BstNSI RCATGY 2 cut(s) 515, 749
BstSCI CCNGG 2 cut(s) 157, 519
BstSFI CTRYAG 1 cut(s) 107
BstV1I GCAGC 4 cut(s) 198, 346, 346, 505
BstX2I RGATCY 1 cut(s) 103
BstYI RGATCY 1 cut(s) 103
BsuRI GGCC 1 cut(s) 727
BtsCI GGATG 2 cut(s) 268, 770
BveI ACCTGC 1 cut(s) 591
Cac8I GCNNGC 2 cut(s) 339, 747
CciI TCATGA 1 cut(s) 289
Cfr13I GGNCC 2 cut(s) 245, 725
CseI GACGC 1 cut(s) 86
Csp6I GTAC 2 cut(s) 54, 155
CviAII CATG 5 cut(s) 290, 490, 512, 688, 746
CviQI GTAC 2 cut(s) 54, 155
DdeI CTNAG 3 cut(s) 88, 342, 551
DpnI GATC 4 cut(s) 99, 105, 429, 525
DpnII GATC 4 cut(s) 97, 103, 427, 523
Ecl136II GAGCTC 2 cut(s) 458, 557
Eco24I GRGCYC 2 cut(s) 460, 559
Eco47I GGWCC 1 cut(s) 245
Eco53kI GAGCTC 2 cut(s) 458, 557
EcoICRI GAGCTC 2 cut(s) 458, 557
EcoNI CCTNNNNNAGG 1 cut(s) 524
EcoRII CCWGG 2 cut(s) 157, 519
EcoT22I ATGCAT 1 cut(s) 747
EcoT38I GRGCYC 2 cut(s) 460, 559
FaeI CATG 5 cut(s) 293, 493, 515, 691, 749
FaiI YATR 7 cut(s) 291, 473, 491, 513, 689, 707, 747
FaqI GGGAC 1 cut(s) 633
FatI CATG 5 cut(s) 289, 489, 511, 687, 745
FauI CCCGC 1 cut(s) 48
Fnu4HI GCNGC 4 cut(s) 212, 335, 360, 494
FokI GGATG 2 cut(s) 255, 757
FriOI GRGCYC 2 cut(s) 460, 559
Fsp4HI GCNGC 4 cut(s) 212, 335, 360, 494
FspBI CTAG 2 cut(s) 225, 461
GluI GCNGC 4 cut(s) 212, 335, 360, 494
GsuI CTGGAG 1 cut(s) 644
HaeIII GGCC 1 cut(s) 727
HgaI GACGC 1 cut(s) 86
Hin1II CATG 5 cut(s) 293, 493, 515, 691, 749
HincII GTYRAC 1 cut(s) 145
HindII GTYRAC 1 cut(s) 145
HindIII AAGCTT 1 cut(s) 348
HinfI GANTC 1 cut(s) 577
HpaI GTTAAC 1 cut(s) 145
HphI GGTGA 3 cut(s) 133, 174, 688
Hpy166II GTNNAC 2 cut(s) 145, 245
Hpy188I TCNGA 7 cut(s) 60, 91, 432, 453, 561, 582, 648
Hpy188III TCNNGA 2 cut(s) 101, 290
Hpy8I GTNNAC 2 cut(s) 145, 245
HpyAV CCTTC 1 cut(s) 726
HpyCH4III ACNGT 3 cut(s) 235, 379, 447
HpyCH4IV ACGT 1 cut(s) 369
HpyCH4V TGCA 8 cut(s) 109, 214, 334, 493, 713, 719, 745, 749
HpyF10VI GCNNNNNNNGC 3 cut(s) 22, 347, 356
HpyF3I CTNAG 3 cut(s) 88, 342, 551
HpySE526I ACGT 1 cut(s) 369
Hsp92II CATG 5 cut(s) 293, 493, 515, 691, 749
KspAI GTTAAC 1 cut(s) 145
Kzo9I GATC 4 cut(s) 97, 103, 427, 523
LmnI GCTCC 1 cut(s) 625
Lsp1109I GCAGC 4 cut(s) 198, 346, 346, 505
LweI GCATC 2 cut(s) 620, 732
MaeI CTAG 2 cut(s) 225, 461
MaeII ACGT 1 cut(s) 369
MalI GATC 4 cut(s) 99, 105, 429, 525
MboI GATC 4 cut(s) 97, 103, 427, 523
MboII GAAGA 5 cut(s) 19, 313, 510, 513, 586
MflI RGATCY 1 cut(s) 103
MhlI GDGCHC 2 cut(s) 460, 559
MluCI AATT 5 cut(s) 401, 639, 665, 679, 737
MmeI TCCRAC 1 cut(s) 398
MnlI CCTC 4 cut(s) 387, 487, 564, 642
Mph1103I ATGCAT 1 cut(s) 747
MroXI GAANNNNTTC 2 cut(s) 369, 656
MseI TTAA 5 cut(s) 144, 306, 404, 596, 781
MspA1I CMGCKG 2 cut(s) 25, 211
MspR9I CCNGG 2 cut(s) 159, 521
Mva1269I GAATGC 1 cut(s) 589
MvaI CCWGG 2 cut(s) 159, 521
MwoI GCNNNNNNNGC 3 cut(s) 22, 347, 356
NdeII GATC 4 cut(s) 97, 103, 427, 523
NlaIII CATG 5 cut(s) 293, 493, 515, 691, 749
NsiI ATGCAT 1 cut(s) 747
NspI RCATGY 2 cut(s) 515, 749
PaeI GCATGC 1 cut(s) 749
PagI TCATGA 1 cut(s) 289
PaqCI CACCTGC 1 cut(s) 591
PciI ACATGT 1 cut(s) 511
PcsI WCGNNNNNNNCGW 1 cut(s) 375
PctI GAATGC 1 cut(s) 589
PdmI GAANNNNTTC 2 cut(s) 369, 656
PfeI GAWTC 1 cut(s) 577
PfoI TCCNGGA 1 cut(s) 519
PkrI GCNGC 4 cut(s) 213, 336, 361, 495
PscI ACATGT 1 cut(s) 511
Psp124BI GAGCTC 2 cut(s) 460, 559
Psp6I CCWGG 2 cut(s) 157, 519
PspGI CCWGG 2 cut(s) 157, 519
PspPI GGNCC 2 cut(s) 245, 725
PstI CTGCAG 1 cut(s) 111
PsuI RGATCY 1 cut(s) 103
PvuII CAGCTG 2 cut(s) 25, 211
RsaI GTAC 2 cut(s) 55, 156
RsaNI GTAC 2 cut(s) 54, 155
SacI GAGCTC 2 cut(s) 460, 559
SaqAI TTAA 5 cut(s) 144, 306, 404, 596, 781
SatI GCNGC 4 cut(s) 212, 335, 360, 494
Sau3AI GATC 4 cut(s) 97, 103, 427, 523
Sau96I GGNCC 2 cut(s) 245, 725
ScaI AGTACT 1 cut(s) 55
ScrFI CCNGG 2 cut(s) 159, 521
SduI GDGCHC 2 cut(s) 460, 559
SfaNI GCATC 2 cut(s) 620, 732
SfcI CTRYAG 1 cut(s) 107
SinI GGWCC 1 cut(s) 245
SphI GCATGC 1 cut(s) 749
Sse9I AATT 5 cut(s) 401, 639, 665, 679, 737
SsiI CCGC 1 cut(s) 41
SspI AATATT 1 cut(s) 565
SspMI CTAG 2 cut(s) 225, 461
SstI GAGCTC 2 cut(s) 460, 559
StyD4I CCNGG 2 cut(s) 157, 519
TaaI ACNGT 3 cut(s) 235, 379, 447
TaiI ACGT 1 cut(s) 372
TaqI TCGA 1 cut(s) 479
TasI AATT 5 cut(s) 401, 639, 665, 679, 737
TatI WGTACW 1 cut(s) 53
TfiI GAWTC 1 cut(s) 577
Tru1I TTAA 5 cut(s) 144, 306, 404, 596, 781
Tru9I TTAA 5 cut(s) 144, 306, 404, 596, 781
TseI GCWGC 4 cut(s) 211, 334, 359, 493
TspDTI ATGAA 3 cut(s) 278, 558, 704
VpaK11BI GGWCC 1 cut(s) 245
XagI CCTNNNNNAGG 1 cut(s) 524
XapI RAATTY 3 cut(s) 639, 665, 679
XceI RCATGY 2 cut(s) 515, 749
XcmI CCANNNNNNNNNTGG 1 cut(s) 280
XmnI GAANNNNTTC 2 cut(s) 369, 656
XspI CTAG 2 cut(s) 225, 461
ZrmI AGTACT 1 cut(s) 55
Zsp2I ATGCAT 1 cut(s) 747
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.