Rorug03G0225300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0013598
Physical Location & Seq
Reverse (-)
20684593 .. 20687531
2939 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0225300.1

Sequence Viewer

Length: 1002 bp
ATGAAATTCGGAGAGGCTTTCACGGAATACCTGCATAACGAACGAGAGCGCTACTTCTTGGAGAAATGCTCGCATGTCGAGTATAAGCGCCTCAAGAAGGTCCTCAAGACTTGTCAGACATTCCATGATGATGAGGCTGAACAAGAAGAGGCGTCTGTTGATCCTGATGATCAAAATCAGCTGTCCTGTCAATGTCAATCTTGCCCTGTGTGTGATCAGACATTCTTCTCAGAGTTGATGAAGGAAGCTTCAGACATAGCTATATGCTTTAATTTGAGAGCCAGACATCTACTCCATCTCCATGTTGCTACTGGAATACAAAGATACTTACTGCGGTTTCGACAATGTTTCAAGTATGATCAACAAGCCATGGTTGAAGAAGGGCGGATGCTGATTGAGTATGCTACAATGAACGCACTTGCTATCAGAAAAATCCTTAAGAAATATGATAAGGTACATTGCTCTGAAAATGGCAAGCATTTCAAATCCAAGTTGCAGGCTGACCATGTTGAGCTTTTGCAATCACCTTGGCTTATAGAACTAGGTGCCTTTTATTTGAATCTTGAGGGATCGGATGGTGGAGAGCTTAATGAGCTTTCTAGTCACTTTTCTTGTGATCTTGATGCAACACCACCGATAATGGCTTTGATTCTTCCCGACTCTCTAAAGATGGAATACGATCTGACTTGTGCTATATGTTTGGAGACTGTATTCAACCCATATGCTTTGAGCTGTGGTCACCTTTTCTGCAAGTCTTGTGCTTGCTCAGCTGCTTCTGTGTTTATCTTCCAAGGCCCAAAAGCTGCAGACTCAGATTCAAAGTGCCCAATATGCAGAGAGACTGGAGTGTATGCTAAAGCAGTGCATATGTTAGAACTAGACCTGCTCCTGAAAAGAAGGTGCAGAGACTATTGGAAAGAGAGGTATACTGCAGAACGTACAGAGACGATGAAGCAAACTAAGGAGTTTTGGGATTTACAAGCCAAATATGCACTGTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

333

Amino Acids

38.56

Weight (kDa)

5.87

Isoelectric Point (pI)

45.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4_3 PF13920 228 - 280 9e-06 Zinc finger, C3HC4 type (RING finger)
RING_XB3-XBAT31 PF24921 229 - 281 1.3e-07 E3 ubiquitin-protein ligase XB3/XBAT31 RING finger
zf-RING_UBOX PF13445 230 - 254 1e-06 RING-type zinc-finger
zf-RING_5 PF14634 230 - 279 7.2e-06 zinc-RING finger domain
zf-C3HC4 PF00097 230 - 278 6.9e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 39, 891
AccB1I GGYRCC 1 cut(s) 545
AccI GTMKAC 1 cut(s) 926
AciI CCGC 2 cut(s) 334, 385
AclWI GGATC 2 cut(s) 155, 577
AcsI RAATTY 1 cut(s) 5
AcuI CTGAAG 1 cut(s) 234
AcyI GRCGYC 1 cut(s) 152
AfaI GTAC 2 cut(s) 456, 940
AfeI AGCGCT 1 cut(s) 50
AfiI CCNNNNNNNGG 1 cut(s) 97
AflII CTTAAG 1 cut(s) 437
AgsI TTSAA 6 cut(s) 352, 377, 484, 559, 715, 819
AluBI AGCT 9 cut(s) 181, 248, 260, 514, 586, 595, 732, 770, 803
AluI AGCT 9 cut(s) 181, 248, 260, 514, 586, 595, 732, 770, 803
Alw26I GTCTC 4 cut(s) 698, 833, 900, 938
AlwI GGATC 2 cut(s) 155, 577
Aor51HI AGCGCT 1 cut(s) 50
AoxI GGCC 1 cut(s) 793
ApeKI GCWGC 2 cut(s) 770, 803
ApoI RAATTY 1 cut(s) 5
AspLEI GCGC 2 cut(s) 51, 90
AspS9I GGNCC 2 cut(s) 100, 794
AsuHPI GGTGA 2 cut(s) 516, 731
AvaII GGWCC 1 cut(s) 100
BaeGI GKGCMC 1 cut(s) 827
BanI GGYRCC 1 cut(s) 545
BbvI GCAGC 2 cut(s) 757, 790
BccI CCATC 3 cut(s) 303, 569, 664
BclI TGATCA 3 cut(s) 169, 214, 358
BcoDI GTCTC 4 cut(s) 698, 833, 900, 938
BfaI CTAG 3 cut(s) 542, 600, 878
BfmI CTRYAG 2 cut(s) 804, 930
BfoI RGCGCY 2 cut(s) 52, 91
BfrI CTTAAG 1 cut(s) 437
BfuAI ACCTGC 2 cut(s) 39, 891
BisI GCNGC 2 cut(s) 771, 804
BlpI GCTNAGC 1 cut(s) 766
BlsI GCNGC 2 cut(s) 772, 805
Bme18I GGWCC 1 cut(s) 100
BmgT120I GGNCC 2 cut(s) 100, 794
BmiI GGNNCC 1 cut(s) 547
BmsI GCATC 2 cut(s) 378, 613
BplI GAGNNNNNCTC 2 cut(s) 53, 85
BpmI CTGGAG 1 cut(s) 864
Bpu1102I GCTNAGC 1 cut(s) 766
BpuEI CTTGAG 3 cut(s) 77, 89, 584
BsaBI GATNNNNATC 1 cut(s) 174
BsaHI GRCGYC 1 cut(s) 152
BsaJI CCNNGG 3 cut(s) 369, 527, 790
BsaXI ACNNNNNCTCC 4 cut(s) 276, 282, 306, 312
Bsc4I CCNNNNNNNGG 1 cut(s) 97
Bse1I ACTGG 2 cut(s) 316, 847
Bse3DI GCAATG 1 cut(s) 457
Bse8I GATNNNNATC 1 cut(s) 174
BseDI CCNNGG 3 cut(s) 369, 527, 790
BseGI GGATG 2 cut(s) 393, 580
BseJI GATNNNNATC 1 cut(s) 174
BseLI CCNNNNNNNGG 1 cut(s) 97
BseMI GCAATG 1 cut(s) 457
BseMII CTCAG 3 cut(s) 243, 780, 825
BseNI ACTGG 2 cut(s) 316, 847
BseSI GKGCMC 1 cut(s) 827
BseXI GCAGC 2 cut(s) 757, 790
BsgI GTGCAG 1 cut(s) 922
BshFI GGCC 1 cut(s) 795
BshNI GGYRCC 1 cut(s) 545
BslI CCNNNNNNNGG 1 cut(s) 97
BsmAI GTCTC 4 cut(s) 698, 833, 900, 938
BsmBI CGTCTC 1 cut(s) 938
BsnI GGCC 1 cut(s) 795
Bsp1286I GDGCHC 1 cut(s) 827
Bsp143I GATC 7 cut(s) 160, 169, 214, 358, 569, 616, 679
Bsp1720I GCTNAGC 1 cut(s) 766
Bsp19I CCATGG 1 cut(s) 369
BspACI CCGC 2 cut(s) 334, 385
BspANI GGCC 1 cut(s) 795
BspCNI CTCAG 3 cut(s) 242, 779, 824
BspLI GGNNCC 1 cut(s) 547
BspMAI CTGCAG 2 cut(s) 808, 934
BspMI ACCTGC 2 cut(s) 39, 891
BspPI GGATC 2 cut(s) 155, 577
BspT107I GGYRCC 1 cut(s) 545
BspTI CTTAAG 1 cut(s) 437
BsrDI GCAATG 1 cut(s) 457
BsrI ACTGG 2 cut(s) 316, 847
BssECI CCNNGG 3 cut(s) 369, 527, 790
BssMI GATC 7 cut(s) 160, 169, 214, 358, 569, 616, 679
BssNAI GTATAC 1 cut(s) 927
BssNI GRCGYC 1 cut(s) 152
BssT1I CCWWGG 3 cut(s) 369, 527, 790
Bst1107I GTATAC 1 cut(s) 927
Bst4CI ACNGT 2 cut(s) 709, 996
Bst6I CTCTTC 1 cut(s) 141
BstACI GRCGYC 1 cut(s) 152
BstAFI CTTAAG 1 cut(s) 437
BstC8I GCNNGC 4 cut(s) 71, 476, 498, 763
BstDEI CTNAG 4 cut(s) 229, 766, 811, 960
BstDSI CCRYGG 1 cut(s) 369
BstEII GGTNACC 1 cut(s) 737
BstENI CCTNNNNNAGG 1 cut(s) 95
BstF5I GGATG 2 cut(s) 393, 580
BstH2I RGCGCY 2 cut(s) 52, 91
BstHHI GCGC 2 cut(s) 51, 90
BstKTI GATC 7 cut(s) 163, 172, 217, 361, 572, 619, 682
BstMAI GTCTC 4 cut(s) 698, 833, 900, 938
BstMBI GATC 7 cut(s) 160, 169, 214, 358, 569, 616, 679
BstMWI GCNNNNNNNGC 4 cut(s) 592, 767, 831, 989
BstNSI RCATGY 1 cut(s) 77
BstPI GGTNACC 1 cut(s) 737
BstSFI CTRYAG 2 cut(s) 804, 930
BstSLI GKGCMC 1 cut(s) 827
BstV1I GCAGC 2 cut(s) 757, 790
BstZ17I GTATAC 1 cut(s) 927
BsuRI GGCC 1 cut(s) 795
BtgI CCRYGG 1 cut(s) 369
BtsCI GGATG 2 cut(s) 393, 580
BtsI GCAGTG 1 cut(s) 867
BtsIMutI CAGTG 2 cut(s) 867, 992
BveI ACCTGC 2 cut(s) 39, 891
Cac8I GCNNGC 4 cut(s) 71, 476, 498, 763
CfoI GCGC 2 cut(s) 51, 90
Cfr13I GGNCC 2 cut(s) 100, 794
CseI GACGC 1 cut(s) 141
Csp6I GTAC 2 cut(s) 455, 939
CviAII CATG 5 cut(s) 74, 125, 302, 370, 506
CviQI GTAC 2 cut(s) 455, 939
DdeI CTNAG 4 cut(s) 229, 766, 811, 960
DpnI GATC 7 cut(s) 162, 171, 216, 360, 571, 618, 681
DpnII GATC 7 cut(s) 160, 169, 214, 358, 569, 616, 679
Eam1104I CTCTTC 1 cut(s) 141
EarI CTCTTC 1 cut(s) 141
EciI GGCGGA 1 cut(s) 400
Eco130I CCWWGG 3 cut(s) 369, 527, 790
Eco47I GGWCC 1 cut(s) 100
Eco47III AGCGCT 1 cut(s) 50
Eco57I CTGAAG 1 cut(s) 234
Eco91I GGTNACC 1 cut(s) 737
EcoNI CCTNNNNNAGG 1 cut(s) 95
EcoO109I RGGNCCY 1 cut(s) 100
EcoO65I GGTNACC 1 cut(s) 737
EcoT14I CCWWGG 3 cut(s) 369, 527, 790
ErhI CCWWGG 3 cut(s) 369, 527, 790
Esp3I CGTCTC 1 cut(s) 938
FaeI CATG 5 cut(s) 77, 128, 305, 373, 509
FatI CATG 5 cut(s) 73, 124, 301, 369, 505
FauNDI CATATG 2 cut(s) 721, 867
FbaI TGATCA 3 cut(s) 169, 214, 358
FblI GTMKAC 1 cut(s) 926
Fnu4HI GCNGC 2 cut(s) 771, 804
FokI GGATG 2 cut(s) 400, 587
Fsp4HI GCNGC 2 cut(s) 771, 804
FspBI CTAG 3 cut(s) 542, 600, 878
GlaI GCGC 2 cut(s) 50, 89
GluI GCNGC 2 cut(s) 771, 804
GsuI CTGGAG 1 cut(s) 864
HaeII RGCGCY 2 cut(s) 52, 91
HaeIII GGCC 1 cut(s) 795
HgaI GACGC 1 cut(s) 141
HhaI GCGC 2 cut(s) 51, 90
Hin1I GRCGYC 1 cut(s) 152
Hin1II CATG 5 cut(s) 77, 128, 305, 373, 509
Hin6I GCGC 2 cut(s) 49, 88
HinP1I GCGC 2 cut(s) 49, 88
HindIII AAGCTT 1 cut(s) 246
HinfI GANTC 5 cut(s) 559, 649, 659, 809, 815
HphI GGTGA 2 cut(s) 516, 731
Hpy166II GTNNAC 1 cut(s) 927
Hpy188III TCNNGA 7 cut(s) 94, 106, 164, 563, 620, 656, 889
Hpy8I GTNNAC 1 cut(s) 927
HpyAV CCTTC 4 cut(s) 91, 235, 374, 891
HpyCH4III ACNGT 2 cut(s) 709, 996
HpyCH4IV ACGT 1 cut(s) 937
HpyF10VI GCNNNNNNNGC 4 cut(s) 592, 767, 831, 989
HpyF3I CTNAG 4 cut(s) 229, 766, 811, 960
HpySE526I ACGT 1 cut(s) 937
Hsp92I GRCGYC 1 cut(s) 152
Hsp92II CATG 5 cut(s) 77, 128, 305, 373, 509
HspAI GCGC 2 cut(s) 49, 88
Ksp22I TGATCA 3 cut(s) 169, 214, 358
Kzo9I GATC 7 cut(s) 160, 169, 214, 358, 569, 616, 679
LmnI GCTCC 1 cut(s) 891
Lsp1109I GCAGC 2 cut(s) 757, 790
LweI GCATC 2 cut(s) 378, 613
MaeI CTAG 3 cut(s) 542, 600, 878
MaeII ACGT 1 cut(s) 937
MaeIII GTNAC 2 cut(s) 602, 737
MalI GATC 7 cut(s) 162, 171, 216, 360, 571, 618, 681
MboI GATC 7 cut(s) 160, 169, 214, 358, 569, 616, 679
MboII GAAGA 5 cut(s) 158, 217, 389, 644, 778
MhlI GDGCHC 1 cut(s) 827
MluCI AATT 2 cut(s) 5, 271
MlyI GAGTC 2 cut(s) 653, 803
MnlI CCTC 7 cut(s) 7, 101, 113, 127, 142, 559, 915
MseI TTAA 3 cut(s) 270, 438, 588
MslI CAYNNNNRTG 2 cut(s) 129, 300
MspA1I CMGCKG 2 cut(s) 181, 770
MspCI CTTAAG 1 cut(s) 437
MwoI GCNNNNNNNGC 4 cut(s) 592, 767, 831, 989
NcoI CCATGG 1 cut(s) 369
NdeI CATATG 2 cut(s) 721, 867
NdeII GATC 7 cut(s) 160, 169, 214, 358, 569, 616, 679
NlaIII CATG 5 cut(s) 77, 128, 305, 373, 509
NlaIV GGNNCC 1 cut(s) 547
NmuCI GTSAC 2 cut(s) 602, 737
NspI RCATGY 1 cut(s) 77
PfeI GAWTC 3 cut(s) 559, 649, 815
PkrI GCNGC 2 cut(s) 772, 805
PleI GAGTC 2 cut(s) 653, 803
PpsI GAGTC 2 cut(s) 653, 803
PpuMI RGGWCCY 1 cut(s) 100
Psp5II RGGWCCY 1 cut(s) 100
PspEI GGTNACC 1 cut(s) 737
PspN4I GGNNCC 1 cut(s) 547
PspPI GGNCC 2 cut(s) 100, 794
PspPPI RGGWCCY 1 cut(s) 100
PstI CTGCAG 2 cut(s) 808, 934
PvuII CAGCTG 2 cut(s) 181, 770
RsaI GTAC 2 cut(s) 456, 940
RsaNI GTAC 2 cut(s) 455, 939
RseI CAYNNNNRTG 2 cut(s) 129, 300
SaqAI TTAA 3 cut(s) 270, 438, 588
SatI GCNGC 2 cut(s) 771, 804
Sau3AI GATC 7 cut(s) 160, 169, 214, 358, 569, 616, 679
Sau96I GGNCC 2 cut(s) 100, 794
SchI GAGTC 2 cut(s) 653, 803
SduI GDGCHC 1 cut(s) 827
SfaNI GCATC 2 cut(s) 378, 613
SfcI CTRYAG 2 cut(s) 804, 930
SinI GGWCC 1 cut(s) 100
SmiMI CAYNNNNRTG 2 cut(s) 129, 300
SmlI CTYRAG 4 cut(s) 92, 104, 437, 563
SmoI CTYRAG 4 cut(s) 92, 104, 437, 563
Sse9I AATT 2 cut(s) 5, 271
SsiI CCGC 2 cut(s) 334, 385
SspMI CTAG 3 cut(s) 542, 600, 878
StyI CCWWGG 3 cut(s) 369, 527, 790
TaaI ACNGT 2 cut(s) 709, 996
TaiI ACGT 1 cut(s) 940
TaqI TCGA 2 cut(s) 78, 340
TasI AATT 2 cut(s) 5, 271
TfiI GAWTC 3 cut(s) 559, 649, 815
Tru1I TTAA 3 cut(s) 270, 438, 588
Tru9I TTAA 3 cut(s) 270, 438, 588
TscAI CASTG 2 cut(s) 867, 999
TseFI GTSAC 2 cut(s) 602, 737
TseI GCWGC 2 cut(s) 770, 803
Tsp45I GTSAC 2 cut(s) 602, 737
TspDTI ATGAA 4 cut(s) 17, 254, 425, 965
TspGWI ACGGA 1 cut(s) 38
TspRI CASTG 2 cut(s) 867, 999
Vha464I CTTAAG 1 cut(s) 437
VpaK11BI GGWCC 1 cut(s) 100
XagI CCTNNNNNAGG 1 cut(s) 95
XapI RAATTY 1 cut(s) 5
XceI RCATGY 1 cut(s) 77
XcmI CCANNNNNNNNNTGG 1 cut(s) 308
XmiI GTMKAC 1 cut(s) 926
XspI CTAG 3 cut(s) 542, 600, 878
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.