Prupe.1G358600_v2.0.a1

mediator of RNA polymerase II transcription subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0009075
Physical Location & Seq
Reverse (-)
33157030 .. 33158593
1564 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G358600.1

Sequence Viewer

Length: 576 bp
ATGGAAGAGAAGGACACAACAAGCCCTAAGACAACGCAAGAGCTAGCAATGGAAGGCCAGAAGCATCTAGAAGAAACCATAGAGGCAGCTTTCCAGATCCTCTCCTCCATGAACGATGAGCTCTGCAACCCCACATTGTGGTCCACCACTTCCTCTTTTTCAGCTGCTACAAACATTGCAAATGGTCCTGCTCCCCACGCAAATGGTGTCAACAATGGCGATTCATCTTCTTCTGATAACACTTCTACCCATCACGGAGACTTAATTGGTGCTGGTGGTTCTGGCACCGGAGGAGCTCTTGAAGAGGCTCGGCATCGGTATACGAACTCTGTGCGCTTACTCCGTGCTGTTCTCACTACGATTCCCAACTCTCAGAAGGCACCATCTGAAACTAGTTCAACTGTAGTCAGTTCAGAATCACCAGCAGATGAAGCTGAAATTGAGAAGTTAGATGAGCGAGCCTCCAATTTGAGAAAGGAGCTTGCAAACAAGAATTTGTATATCAAGTTTCTTATAGATCAACTACGAGATCTTATTACTGACATATCAACATGGCAAAGTCCTTGTTCCATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0016592 GO:0031974 GO:0031981 GO:0032991 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0070013
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

192

Amino Acids

20.54

Weight (kDa)

4.76

Isoelectric Point (pI)

47.61

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 284, 379
AccB7I CCANNNNNTGG 1 cut(s) 138
AccI GTMKAC 1 cut(s) 320
AclWI GGATC 1 cut(s) 91
AcsI RAATTY 1 cut(s) 493
AdeI CACNNNGTG 1 cut(s) 138
AfiI CCNNNNNNNGG 1 cut(s) 138
AgsI TTSAA 2 cut(s) 302, 399
AhlI ACTAGT 1 cut(s) 392
AluBI AGCT 7 cut(s) 43, 89, 121, 164, 296, 434, 481
AluI AGCT 7 cut(s) 43, 89, 121, 164, 296, 434, 481
Alw21I GWGCWC 2 cut(s) 123, 298
Alw26I GTCTC 1 cut(s) 252
AlwI GGATC 1 cut(s) 91
AoxI GGCC 1 cut(s) 55
ApeKI GCWGC 2 cut(s) 86, 164
ApoI RAATTY 1 cut(s) 493
AspLEI GCGC 1 cut(s) 336
AspS9I GGNCC 2 cut(s) 141, 185
AsuHPI GGTGA 1 cut(s) 411
AsuNHI GCTAGC 1 cut(s) 43
AvaII GGWCC 2 cut(s) 141, 185
BanI GGYRCC 2 cut(s) 284, 379
BanII GRGCYC 2 cut(s) 123, 298
Bbv12I GWGCWC 2 cut(s) 123, 298
BbvI GCAGC 2 cut(s) 98, 151
BccI CCATC 2 cut(s) 258, 391
BcgI CGANNNNNNTGC 2 cut(s) 313, 347
BcoDI GTCTC 1 cut(s) 252
BcuI ACTAGT 1 cut(s) 392
BfaI CTAG 3 cut(s) 44, 68, 393
BfmI CTRYAG 1 cut(s) 402
BglII AGATCT 1 cut(s) 529
BisI GCNGC 2 cut(s) 87, 165
BlsI GCNGC 2 cut(s) 88, 166
Bme18I GGWCC 2 cut(s) 141, 185
BmgT120I GGNCC 2 cut(s) 141, 185
BmiI GGNNCC 2 cut(s) 286, 381
BmsI GCATC 2 cut(s) 73, 322
BmtI GCTAGC 1 cut(s) 47
BplI GAGNNNNNCTC 2 cut(s) 446, 478
BsaWI WCCGGW 1 cut(s) 287
Bsc4I CCNNNNNNNGG 1 cut(s) 138
Bse3DI GCAATG 2 cut(s) 54, 174
BseLI CCNNNNNNNGG 1 cut(s) 138
BseMI GCAATG 2 cut(s) 54, 174
BseMII CTCAG 1 cut(s) 386
BseRI GAGGAG 2 cut(s) 94, 306
BseXI GCAGC 2 cut(s) 98, 151
BshFI GGCC 1 cut(s) 57
BshNI GGYRCC 2 cut(s) 284, 379
BsiHKAI GWGCWC 2 cut(s) 123, 298
BsiSI CCGG 1 cut(s) 288
BslI CCNNNNNNNGG 1 cut(s) 138
BsmAI GTCTC 1 cut(s) 252
BsnI GGCC 1 cut(s) 57
Bsp1286I GDGCHC 2 cut(s) 123, 298
Bsp143I GATC 3 cut(s) 96, 517, 529
BspANI GGCC 1 cut(s) 57
BspCNI CTCAG 1 cut(s) 385
BspLI GGNNCC 2 cut(s) 286, 381
BspOI GCTAGC 1 cut(s) 47
BspPI GGATC 1 cut(s) 91
BspT107I GGYRCC 2 cut(s) 284, 379
BsrDI GCAATG 2 cut(s) 54, 174
BssMI GATC 3 cut(s) 96, 517, 529
BssNAI GTATAC 1 cut(s) 321
Bst1107I GTATAC 1 cut(s) 321
Bst4CI ACNGT 1 cut(s) 403
Bst6I CTCTTC 1 cut(s) 297
BstC8I GCNNGC 3 cut(s) 45, 459, 483
BstDEI CTNAG 2 cut(s) 27, 372
BstHHI GCGC 1 cut(s) 336
BstKTI GATC 3 cut(s) 99, 520, 532
BstMAI GTCTC 1 cut(s) 252
BstMBI GATC 3 cut(s) 96, 517, 529
BstMWI GCNNNNNNNGC 2 cut(s) 197, 431
BstSFI CTRYAG 1 cut(s) 402
BstV1I GCAGC 2 cut(s) 98, 151
BstX2I RGATCY 2 cut(s) 96, 529
BstXI CCANNNNNNTGG 1 cut(s) 203
BstYI RGATCY 2 cut(s) 96, 529
BstZ17I GTATAC 1 cut(s) 321
BsuRI GGCC 1 cut(s) 57
Cac8I GCNNGC 3 cut(s) 45, 459, 483
CfoI GCGC 1 cut(s) 336
Cfr13I GGNCC 2 cut(s) 141, 185
CviAII CATG 2 cut(s) 109, 552
DdeI CTNAG 2 cut(s) 27, 372
DpnI GATC 3 cut(s) 98, 519, 531
DpnII GATC 3 cut(s) 96, 517, 529
DraIII CACNNNGTG 1 cut(s) 138
Eam1104I CTCTTC 1 cut(s) 297
EarI CTCTTC 1 cut(s) 297
Ecl136II GAGCTC 2 cut(s) 121, 296
Eco24I GRGCYC 2 cut(s) 123, 298
Eco47I GGWCC 2 cut(s) 141, 185
Eco53kI GAGCTC 2 cut(s) 121, 296
EcoICRI GAGCTC 2 cut(s) 121, 296
EcoT38I GRGCYC 2 cut(s) 123, 298
FaeI CATG 2 cut(s) 112, 555
FaiI YATR 7 cut(s) 80, 110, 321, 501, 515, 545, 553
FatI CATG 2 cut(s) 108, 551
FblI GTMKAC 1 cut(s) 320
Fnu4HI GCNGC 2 cut(s) 87, 165
FriOI GRGCYC 2 cut(s) 123, 298
Fsp4HI GCNGC 2 cut(s) 87, 165
FspBI CTAG 3 cut(s) 44, 68, 393
GlaI GCGC 1 cut(s) 335
GluI GCNGC 2 cut(s) 87, 165
HaeIII GGCC 1 cut(s) 57
HapII CCGG 1 cut(s) 288
HhaI GCGC 1 cut(s) 336
Hin1II CATG 2 cut(s) 112, 555
Hin6I GCGC 1 cut(s) 334
HinP1I GCGC 1 cut(s) 334
HincII GTYRAC 1 cut(s) 211
HindII GTYRAC 1 cut(s) 211
HinfI GANTC 3 cut(s) 221, 361, 416
HpaII CCGG 1 cut(s) 288
HphI GGTGA 1 cut(s) 411
Hpy166II GTNNAC 3 cut(s) 144, 211, 321
Hpy188I TCNGA 5 cut(s) 235, 375, 388, 415, 575
Hpy188III TCNNGA 3 cut(s) 68, 94, 299
Hpy8I GTNNAC 3 cut(s) 144, 211, 321
HpyAV CCTTC 3 cut(s) 4, 47, 370
HpyCH4III ACNGT 1 cut(s) 403
HpyCH4V TGCA 3 cut(s) 126, 179, 485
HpyF10VI GCNNNNNNNGC 2 cut(s) 197, 431
HpyF3I CTNAG 2 cut(s) 27, 372
Hsp92II CATG 2 cut(s) 112, 555
HspAI GCGC 1 cut(s) 334
Kzo9I GATC 3 cut(s) 96, 517, 529
LmnI GCTCC 3 cut(s) 196, 293, 478
LpnPI CCDG 7 cut(s) 71, 107, 201, 258, 267, 301, 435
Lsp1109I GCAGC 2 cut(s) 98, 151
LweI GCATC 2 cut(s) 73, 322
MaeI CTAG 3 cut(s) 44, 68, 393
MalI GATC 3 cut(s) 98, 519, 531
MboI GATC 3 cut(s) 96, 517, 529
MboII GAAGA 5 cut(s) 17, 83, 219, 222, 314
MflI RGATCY 2 cut(s) 96, 529
MhlI GDGCHC 2 cut(s) 123, 298
MluCI AATT 4 cut(s) 264, 438, 466, 493
MnlI CCTC 7 cut(s) 76, 110, 115, 163, 284, 298, 472
MseI TTAA 1 cut(s) 263
MslI CAYNNNNRTG 1 cut(s) 201
MspA1I CMGCKG 1 cut(s) 164
MspI CCGG 1 cut(s) 288
MwoI GCNNNNNNNGC 2 cut(s) 197, 431
NdeII GATC 3 cut(s) 96, 517, 529
NheI GCTAGC 1 cut(s) 43
NlaIII CATG 2 cut(s) 112, 555
NlaIV GGNNCC 2 cut(s) 286, 381
NmeAIII GCCGAG 1 cut(s) 289
PfeI GAWTC 3 cut(s) 221, 361, 416
PflMI CCANNNNNTGG 1 cut(s) 138
PkrI GCNGC 2 cut(s) 88, 166
Psp124BI GAGCTC 2 cut(s) 123, 298
PspN4I GGNNCC 2 cut(s) 286, 381
PspPI GGNCC 2 cut(s) 141, 185
PsuI RGATCY 2 cut(s) 96, 529
PvuII CAGCTG 1 cut(s) 164
RseI CAYNNNNRTG 1 cut(s) 201
SacI GAGCTC 2 cut(s) 123, 298
SaqAI TTAA 1 cut(s) 263
SatI GCNGC 2 cut(s) 87, 165
Sau3AI GATC 3 cut(s) 96, 517, 529
Sau96I GGNCC 2 cut(s) 141, 185
SduI GDGCHC 2 cut(s) 123, 298
SetI ASST 7 cut(s) 45, 91, 123, 166, 298, 436, 483
SfaNI GCATC 2 cut(s) 73, 322
SfcI CTRYAG 1 cut(s) 402
SinI GGWCC 2 cut(s) 141, 185
SmiMI CAYNNNNRTG 1 cut(s) 201
SpeI ACTAGT 1 cut(s) 392
Sse9I AATT 4 cut(s) 264, 438, 466, 493
SspMI CTAG 3 cut(s) 44, 68, 393
SstI GAGCTC 2 cut(s) 123, 298
TaaI ACNGT 1 cut(s) 403
TasI AATT 4 cut(s) 264, 438, 466, 493
TfiI GAWTC 3 cut(s) 221, 361, 416
Tru1I TTAA 1 cut(s) 263
Tru9I TTAA 1 cut(s) 263
TseI GCWGC 2 cut(s) 86, 164
TspDTI ATGAA 3 cut(s) 125, 213, 444
TspGWI ACGGA 2 cut(s) 270, 332
Van91I CCANNNNNTGG 1 cut(s) 138
VpaK11BI GGWCC 2 cut(s) 141, 185
XapI RAATTY 1 cut(s) 493
XbaI TCTAGA 1 cut(s) 67
XmiI GTMKAC 1 cut(s) 320
XspI CTAG 3 cut(s) 44, 68, 393
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.