Prupe.2G214200_v2.0.a1

Tryptophan/tyrosine permease family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0013714
Physical Location & Seq
Reverse (-)
24534131 .. 24537169
3039 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G214200.3

Sequence Viewer

Length: 1539 bp
ATGGACTCAAAATGCATGACCTCCTTCCATCCCAGAAGAAGCCACAAAAACCCAAACACAAATCAATCCTGGGCAAGAAGCATTCACCAAAACAACCGAAGTGATTTCATGCGTATACAACTCAAGATCAGTAGCAGTAGCAGGAGCAAGAGCAGCTGCAGCTACCATGTTTGTTCCTCTCACCAACAGTTGCAGCAGCACAAGTCATTGCTTGCTCAAAAGGCTTCTGCAAAGAAGGCAAAGACAGGTCCTATCAAGGAGCCCAGGAAGAAAGGAACTATTGCTGGGGCAATTGCTTTGATCATTGGTACTAGTATAGGCTCAGGGATCCTTGCACTCCCAGAAAAAGCATCTCCAGCAGGATTGCTTCCAAGTTCAATATCCATCGTGGTATGTTGGGGGTTTCTCCTAATTGAGGCCCTTTTGCTTGTTGAAATCAATGTGAGCATGAGGAGGAAGCAGGGGAAGGAAGACAGAGAGAATGAATTGGAGGTCATTTCCATTAGGACTATGGCACAAGAGACTCTGGGAGATTGGGGTGGAACTTTAGTCACTGTGATTTATATTTTCTTGGGCTATACTTCAGTCATTGCTTATAGTTCCAAGTCTGGAGAGATCCTCTTCCATTTGATCAATCTCCCAGCTCCAGTTTCAGGCTTCTTTTTTACTTCACTTTTTACTCTGCTCATTGCCATAGGCGGGACTCGAGCTACTGATCAAGTTAACCAACTGCTCACTGCTTCCATGATAGGTTTACTACTAGCAATAGAGGTGCTGGCAGTTATGATTGGAGGATGGTCAGGACTAGAGGGAACTGGAGACTGGGGCAAAGTCCCGGCTACAATACCTGTGATAATCTTTTCTTTGGTTTATCATGACCTTGCACCTGTTCTTTGTGCTTATTTGGGCGGTGATCTCACACGCTTAAGGATTTCAGTATTTCTTGGTAGTCTTGTTCCATTGCTGGCATTGCTTGTTTGGGATGCAATTGCACTTGGCCTCTCTGCCCATGCTGATCAAGTTGTTGACCCTGTTGAATTGCTTATGAGGGTGAGATGGAGTTGGGTTTCATATATGGTAGAAGCTTTCTCACTCCTTGCTGTTGGAACATCACTAATTGGGACCCTTCTAGGCTTCTCTGAATTTTTCAAGGAACAGCTTAATAACCTATCATGGAATTCTCCCCTCACACAAACACTCCAGAAACCAAACATCTCCTTTGAGCTGAGAAAATGGTGGGGAAGGAATAAAGTTAGCTTCACAGCAGTGGCAATGGTTGTTGGTCCATCTCTTATCGTGTCAACTAGCGTTCCAGATGCATTCTCCACTGCCACAGACATTGCTGGAGGCTACTGTATGACAGTGCTATATGGAGTTCTGCCACCAGCAATGGCTTGGGCAATGCACAATAAAGAATGTGAAAATGGTGAGCAAAAGACTTTATCAAGAGCAAGGCCTGTACTTCTTGGGGTAGGACTATTAGCTTGCGCAATAGTGGCCGAGCAAATCCTGCAGGATATCCTCACATTTCATTCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Trp_Tyr_perm

Protein Analysis

513

Amino Acids

55.66

Weight (kDa)

9.01

Isoelectric Point (pI)

44.2

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013714)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g19650 FvH4_7g19650
malus_domestica MD07G1175000.v1.1
prunus_persica Prupe.2G214200_v2.0.a1 Prupe.2G214200_v2.0.a1 Prupe.2G214200_v2.0.a1
pyrus_communis pycom07g16940
rosa_chinensis RchiOBHm_Chr1g0363461
rosa_laevigata RLG00000027564
rosa_multiflora Rmu_sc0004458.1_g000002
rosa_roxburghii Rroxscaffold_4G00293160
rosa_rugosa Rorug01G0306900 Rorug01G0307000
rosa_samantha Rh1AG316100 Rh1BG279400 Rh1CG295700 Rh1DG310500
rosa_wichuraiana Rw1G027990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1489
AccI GTMKAC 1 cut(s) 115
AciI CCGC 2 cut(s) 699, 909
AclWI GGATC 3 cut(s) 322, 335, 610
AcoI YGGCCR 1 cut(s) 1497
AcsI RAATTY 2 cut(s) 1142, 1177
AcuI CTGAAG 1 cut(s) 567
AfaI GTAC 2 cut(s) 310, 1461
AfiI CCNNNNNNNGG 3 cut(s) 415, 653, 699
AflII CTTAAG 1 cut(s) 925
AgsI TTSAA 4 cut(s) 378, 434, 1037, 1150
AhlI ACTAGT 1 cut(s) 311
AjnI CCWGG 2 cut(s) 68, 263
AleI CACNNNNGTG 1 cut(s) 1265
AluBI AGCT 9 cut(s) 156, 162, 644, 710, 1085, 1159, 1225, 1257, 1484
AluI AGCT 9 cut(s) 156, 162, 644, 710, 1085, 1159, 1225, 1257, 1484
Alw26I GTCTC 2 cut(s) 515, 813
AlwI GGATC 3 cut(s) 322, 335, 610
Ama87I CYCGRG 1 cut(s) 705
AoxI GGCC 4 cut(s) 417, 997, 1454, 1497
ApeKI GCWGC 5 cut(s) 153, 156, 159, 193, 196
ApoI RAATTY 2 cut(s) 1142, 1177
AspLEI GCGC 1 cut(s) 1490
AspS9I GGNCC 4 cut(s) 248, 418, 1122, 1283
AsuC2I CCSGG 1 cut(s) 836
AsuHPI GGTGA 5 cut(s) 77, 173, 923, 1063, 1439
AvaI CYCGRG 1 cut(s) 705
AvaII GGWCC 3 cut(s) 248, 1122, 1283
BamHI GGATCC 1 cut(s) 327
BanII GRGCYC 1 cut(s) 264
BbsI GAAGAC 1 cut(s) 477
BbvI GCAGC 5 cut(s) 143, 165, 171, 205, 208
BccI CCATC 5 cut(s) 36, 392, 789, 1050, 1294
BciT130I CCWGG 2 cut(s) 70, 265
BclI TGATCA 4 cut(s) 300, 630, 715, 1015
BcnI CCSGG 1 cut(s) 836
BcoDI GTCTC 2 cut(s) 515, 813
BcuI ACTAGT 1 cut(s) 311
BfaI CTAG 6 cut(s) 312, 761, 806, 1130, 1305, 1537
BfmI CTRYAG 2 cut(s) 157, 1511
BfrI CTTAAG 1 cut(s) 925
BisI GCNGC 5 cut(s) 154, 157, 160, 194, 197
BlsI GCNGC 5 cut(s) 155, 158, 161, 195, 198
Bme1390I CCNGG 3 cut(s) 70, 265, 836
Bme18I GGWCC 3 cut(s) 248, 1122, 1283
BmeT110I CYCGRG 1 cut(s) 705
BmgT120I GGNCC 4 cut(s) 248, 418, 1122, 1283
BmiI GGNNCC 4 cut(s) 261, 329, 1123, 1124
BmrFI CCNGG 3 cut(s) 70, 265, 836
BmrI ACTGGG 1 cut(s) 832
BmsI GCATC 3 cut(s) 359, 973, 1306
BmuI ACTGGG 1 cut(s) 832
BpiI GAAGAC 1 cut(s) 477
BplI GAGNNNNNCTC 2 cut(s) 603, 635
BpmI CTGGAG 6 cut(s) 339, 630, 630, 837, 1184, 1365
Bpu10I CCTNAGC 1 cut(s) 322
BpuEI CTTGAG 1 cut(s) 107
BpuMI CCSGG 1 cut(s) 836
BsaJI CCNNGG 2 cut(s) 69, 263
BsaXI ACNNNNNCTCC 2 cut(s) 1182, 1212
Bsc4I CCNNNNNNNGG 3 cut(s) 415, 653, 699
Bse1I ACTGG 3 cut(s) 647, 820, 827
Bse3DI GCAATG 9 cut(s) 206, 588, 687, 959, 968, 1278, 1338, 1395, 1407
BseBI CCWGG 2 cut(s) 70, 265
BseDI CCNNGG 2 cut(s) 69, 263
BseGI GGATG 3 cut(s) 28, 800, 988
BseLI CCNNNNNNNGG 3 cut(s) 415, 653, 699
BseMI GCAATG 9 cut(s) 206, 588, 687, 959, 968, 1278, 1338, 1395, 1407
BseMII CTCAG 2 cut(s) 336, 1217
BseNI ACTGG 3 cut(s) 647, 820, 827
BseRI GAGGAG 1 cut(s) 466
BseXI GCAGC 5 cut(s) 143, 165, 171, 205, 208
BseYI CCCAGC 2 cut(s) 284, 640
BshFI GGCC 4 cut(s) 419, 999, 1456, 1499
BsiHKCI CYCGRG 1 cut(s) 705
BsiSI CCGG 1 cut(s) 836
BslFI GGGAC 3 cut(s) 715, 818, 1135
BslI CCNNNNNNNGG 3 cut(s) 415, 653, 699
BsmAI GTCTC 2 cut(s) 515, 813
BsmFI GGGAC 3 cut(s) 715, 818, 1135
BsmI GAATGC 2 cut(s) 81, 1319
BsnI GGCC 4 cut(s) 419, 999, 1456, 1499
BsoBI CYCGRG 1 cut(s) 705
Bsp1286I GDGCHC 1 cut(s) 264
Bsp143I GATC 8 cut(s) 126, 300, 327, 615, 630, 715, 913, 1015
BspACI CCGC 2 cut(s) 699, 909
BspANI GGCC 4 cut(s) 419, 999, 1456, 1499
BspCNI CTCAG 2 cut(s) 335, 1218
BspHI TCATGA 1 cut(s) 874
BspLI GGNNCC 4 cut(s) 261, 329, 1123, 1124
BspMAI CTGCAG 2 cut(s) 161, 1515
BspPI GGATC 3 cut(s) 322, 335, 610
BspTI CTTAAG 1 cut(s) 925
BsrDI GCAATG 9 cut(s) 206, 588, 687, 959, 968, 1278, 1338, 1395, 1407
BsrI ACTGG 3 cut(s) 647, 820, 827
BssECI CCNNGG 2 cut(s) 69, 263
BssMI GATC 8 cut(s) 126, 300, 327, 615, 630, 715, 913, 1015
BssNAI GTATAC 1 cut(s) 116
Bst1107I GTATAC 1 cut(s) 116
Bst2UI CCWGG 2 cut(s) 70, 265
Bst4CI ACNGT 4 cut(s) 189, 556, 1355, 1363
Bst6I CTCTTC 1 cut(s) 626
BstAFI CTTAAG 1 cut(s) 925
BstAPI GCANNNNNTGC 1 cut(s) 1510
BstC8I GCNNGC 4 cut(s) 213, 777, 966, 1486
BstDEI CTNAG 2 cut(s) 322, 1226
BstENI CCTNNNNNAGG 1 cut(s) 413
BstF5I GGATG 3 cut(s) 28, 800, 988
BstHHI GCGC 1 cut(s) 1490
BstKTI GATC 8 cut(s) 129, 303, 330, 618, 633, 718, 916, 1018
BstMAI GTCTC 2 cut(s) 515, 813
BstMBI GATC 8 cut(s) 126, 300, 327, 615, 630, 715, 913, 1015
BstMWI GCNNNNNNNGC 8 cut(s) 153, 159, 221, 236, 356, 970, 1496, 1510
BstNI CCWGG 2 cut(s) 70, 265
BstSCI CCNGG 3 cut(s) 68, 263, 834
BstSFI CTRYAG 2 cut(s) 157, 1511
BstV1I GCAGC 5 cut(s) 143, 165, 171, 205, 208
BstV2I GAAGAC 1 cut(s) 477
BstX2I RGATCY 2 cut(s) 327, 615
BstYI RGATCY 2 cut(s) 327, 615
BstZ17I GTATAC 1 cut(s) 116
BsuRI GGCC 4 cut(s) 419, 999, 1456, 1499
BtsCI GGATG 3 cut(s) 28, 800, 988
BtsI GCAGTG 3 cut(s) 735, 1272, 1326
BtsIMutI CAGTG 5 cut(s) 552, 735, 1272, 1326, 1368
Cac8I GCNNGC 4 cut(s) 213, 777, 966, 1486
CciI TCATGA 1 cut(s) 874
CfoI GCGC 1 cut(s) 1490
Cfr13I GGNCC 4 cut(s) 248, 418, 1122, 1283
Csp6I GTAC 2 cut(s) 309, 1460
CspCI CAANNNNNGTGG 2 cut(s) 1321, 1356
CviAII CATG 8 cut(s) 16, 109, 167, 448, 745, 875, 1010, 1173
CviQI GTAC 2 cut(s) 309, 1460
DdeI CTNAG 2 cut(s) 322, 1226
DpnI GATC 8 cut(s) 128, 302, 329, 617, 632, 717, 915, 1017
DpnII GATC 8 cut(s) 126, 300, 327, 615, 630, 715, 913, 1015
EaeI YGGCCR 1 cut(s) 1497
Eam1104I CTCTTC 1 cut(s) 626
EarI CTCTTC 1 cut(s) 626
Eco147I AGGCCT 1 cut(s) 1456
Eco24I GRGCYC 1 cut(s) 264
Eco32I GATATC 1 cut(s) 1519
Eco47I GGWCC 3 cut(s) 248, 1122, 1283
Eco57I CTGAAG 1 cut(s) 567
Eco88I CYCGRG 1 cut(s) 705
EcoNI CCTNNNNNAGG 1 cut(s) 413
EcoO109I RGGNCCY 3 cut(s) 248, 418, 1122
EcoRI GAATTC 1 cut(s) 1177
EcoRII CCWGG 2 cut(s) 68, 263
EcoRV GATATC 1 cut(s) 1519
EcoT22I ATGCAT 2 cut(s) 17, 1321
EcoT38I GRGCYC 1 cut(s) 264
FaeI CATG 8 cut(s) 19, 112, 170, 451, 748, 878, 1013, 1176
FaqI GGGAC 3 cut(s) 715, 818, 1135
FatI CATG 8 cut(s) 15, 108, 166, 447, 744, 874, 1009, 1172
FauI CCCGC 1 cut(s) 692
FbaI TGATCA 4 cut(s) 300, 630, 715, 1015
FblI GTMKAC 1 cut(s) 115
Fnu4HI GCNGC 5 cut(s) 154, 157, 160, 194, 197
FokI GGATG 3 cut(s) 15, 807, 995
FriOI GRGCYC 1 cut(s) 264
Fsp4HI GCNGC 5 cut(s) 154, 157, 160, 194, 197
FspBI CTAG 6 cut(s) 312, 761, 806, 1130, 1305, 1537
FspI TGCGCA 1 cut(s) 1489
GlaI GCGC 1 cut(s) 1489
GluI GCNGC 5 cut(s) 154, 157, 160, 194, 197
GsaI CCCAGC 2 cut(s) 288, 644
GsuI CTGGAG 6 cut(s) 339, 630, 630, 837, 1184, 1365
HaeIII GGCC 4 cut(s) 419, 999, 1456, 1499
HapII CCGG 1 cut(s) 836
HhaI GCGC 1 cut(s) 1490
Hin1II CATG 8 cut(s) 19, 112, 170, 451, 748, 878, 1013, 1176
Hin6I GCGC 1 cut(s) 1488
HinP1I GCGC 1 cut(s) 1488
HincII GTYRAC 3 cut(s) 724, 1027, 1302
HindII GTYRAC 3 cut(s) 724, 1027, 1302
HindIII AAGCTT 1 cut(s) 1083
HinfI GANTC 3 cut(s) 5, 523, 703
HpaI GTTAAC 1 cut(s) 724
HpaII CCGG 1 cut(s) 836
HphI GGTGA 5 cut(s) 77, 173, 923, 1063, 1439
Hpy166II GTNNAC 5 cut(s) 116, 724, 755, 1027, 1302
Hpy188I TCNGA 1 cut(s) 1141
Hpy188III TCNNGA 7 cut(s) 124, 609, 801, 875, 1201, 1313, 1446
Hpy8I GTNNAC 5 cut(s) 116, 724, 755, 1027, 1302
HpyAV CCTTC 5 cut(s) 34, 229, 460, 1136, 1236
HpyCH4III ACNGT 4 cut(s) 189, 556, 1355, 1363
HpyF10VI GCNNNNNNNGC 8 cut(s) 153, 159, 221, 236, 356, 970, 1496, 1510
HpyF3I CTNAG 2 cut(s) 322, 1226
Hsp92II CATG 8 cut(s) 19, 112, 170, 451, 748, 878, 1013, 1176
HspAI GCGC 1 cut(s) 1488
KflI GGGWCCC 1 cut(s) 1122
Ksp22I TGATCA 4 cut(s) 300, 630, 715, 1015
KspAI GTTAAC 1 cut(s) 724
Kzo9I GATC 8 cut(s) 126, 300, 327, 615, 630, 715, 913, 1015
LmnI GCTCC 3 cut(s) 144, 259, 649
Lsp1109I GCAGC 5 cut(s) 143, 165, 171, 205, 208
LweI GCATC 3 cut(s) 359, 973, 1306
MaeI CTAG 6 cut(s) 312, 761, 806, 1130, 1305, 1537
MaeIII GTNAC 1 cut(s) 550
MalI GATC 8 cut(s) 128, 302, 329, 617, 632, 717, 915, 1017
MboI GATC 8 cut(s) 126, 300, 327, 615, 630, 715, 913, 1015
MboII GAAGA 4 cut(s) 48, 280, 482, 613
MfeI CAATTG 2 cut(s) 291, 987
MflI RGATCY 2 cut(s) 327, 615
MhlI GDGCHC 1 cut(s) 264
MluCI AATT 8 cut(s) 291, 411, 485, 987, 1037, 1116, 1142, 1177
MlyI GAGTC 2 cut(s) 517, 697
MmeI TCCRAC 1 cut(s) 1084
Mph1103I ATGCAT 2 cut(s) 17, 1321
MseI TTAA 3 cut(s) 723, 926, 1161
MslI CAYNNNNRTG 1 cut(s) 1265
MspA1I CMGCKG 1 cut(s) 156
MspCI CTTAAG 1 cut(s) 925
MspI CCGG 1 cut(s) 836
MspR9I CCNGG 3 cut(s) 70, 265, 836
MunI CAATTG 2 cut(s) 291, 987
Mva1269I GAATGC 2 cut(s) 81, 1319
MvaI CCWGG 2 cut(s) 70, 265
MwoI GCNNNNNNNGC 8 cut(s) 153, 159, 221, 236, 356, 970, 1496, 1510
NciI CCSGG 1 cut(s) 836
NdeII GATC 8 cut(s) 126, 300, 327, 615, 630, 715, 913, 1015
NlaIII CATG 8 cut(s) 19, 112, 170, 451, 748, 878, 1013, 1176
NlaIV GGNNCC 4 cut(s) 261, 329, 1123, 1124
NmeAIII GCCGAG 1 cut(s) 1525
NmuCI GTSAC 1 cut(s) 550
NsbI TGCGCA 1 cut(s) 1489
NsiI ATGCAT 2 cut(s) 17, 1321
OliI CACNNNNGTG 1 cut(s) 1265
PaeR7I CTCGAG 1 cut(s) 705
PagI TCATGA 1 cut(s) 874
PceI AGGCCT 1 cut(s) 1456
PctI GAATGC 2 cut(s) 81, 1319
PkrI GCNGC 5 cut(s) 155, 158, 161, 195, 198
PleI GAGTC 2 cut(s) 517, 697
PpsI GAGTC 2 cut(s) 517, 697
PpuMI RGGWCCY 2 cut(s) 248, 1122
Psp5II RGGWCCY 2 cut(s) 248, 1122
Psp6I CCWGG 2 cut(s) 68, 263
PspFI CCCAGC 2 cut(s) 284, 640
PspGI CCWGG 2 cut(s) 68, 263
PspN4I GGNNCC 4 cut(s) 261, 329, 1123, 1124
PspPI GGNCC 4 cut(s) 248, 418, 1122, 1283
PspPPI RGGWCCY 2 cut(s) 248, 1122
PspXI VCTCGAGB 1 cut(s) 705
PstI CTGCAG 2 cut(s) 161, 1515
PsuI RGATCY 2 cut(s) 327, 615
PvuII CAGCTG 1 cut(s) 156
RsaI GTAC 2 cut(s) 310, 1461
RsaNI GTAC 2 cut(s) 309, 1460
RseI CAYNNNNRTG 1 cut(s) 1265
SaqAI TTAA 3 cut(s) 723, 926, 1161
SatI GCNGC 5 cut(s) 154, 157, 160, 194, 197
Sau3AI GATC 8 cut(s) 126, 300, 327, 615, 630, 715, 913, 1015
Sau96I GGNCC 4 cut(s) 248, 418, 1122, 1283
SbfI CCTGCAGG 1 cut(s) 1515
SchI GAGTC 2 cut(s) 517, 697
ScrFI CCNGG 3 cut(s) 70, 265, 836
SdaI CCTGCAGG 1 cut(s) 1515
SduI GDGCHC 1 cut(s) 264
SfaNI GCATC 3 cut(s) 359, 973, 1306
SfcI CTRYAG 2 cut(s) 157, 1511
Sfr274I CTCGAG 1 cut(s) 705
SinI GGWCC 3 cut(s) 248, 1122, 1283
SlaI CTCGAG 1 cut(s) 705
SmiMI CAYNNNNRTG 1 cut(s) 1265
SmlI CTYRAG 3 cut(s) 122, 705, 925
SmoI CTYRAG 3 cut(s) 122, 705, 925
SpeI ACTAGT 1 cut(s) 311
Sse8387I CCTGCAGG 1 cut(s) 1515
Sse9I AATT 8 cut(s) 291, 411, 485, 987, 1037, 1116, 1142, 1177
SseBI AGGCCT 1 cut(s) 1456
SsiI CCGC 2 cut(s) 699, 909
SspMI CTAG 6 cut(s) 312, 761, 806, 1130, 1305, 1537
StuI AGGCCT 1 cut(s) 1456
StyD4I CCNGG 3 cut(s) 68, 263, 834
TaaI ACNGT 4 cut(s) 189, 556, 1355, 1363
TaqI TCGA 1 cut(s) 706
TasI AATT 8 cut(s) 291, 411, 485, 987, 1037, 1116, 1142, 1177
TatI WGTACW 1 cut(s) 1459
Tru1I TTAA 3 cut(s) 723, 926, 1161
Tru9I TTAA 3 cut(s) 723, 926, 1161
TscAI CASTG 5 cut(s) 559, 742, 1272, 1333, 1368
TseFI GTSAC 1 cut(s) 550
TseI GCWGC 5 cut(s) 153, 156, 159, 193, 196
Tsp45I GTSAC 1 cut(s) 550
TspDTI ATGAA 4 cut(s) 97, 498, 1059, 1520
TspRI CASTG 5 cut(s) 559, 742, 1272, 1333, 1368
Vha464I CTTAAG 1 cut(s) 925
VpaK11BI GGWCC 3 cut(s) 248, 1122, 1283
XagI CCTNNNNNAGG 1 cut(s) 413
XapI RAATTY 2 cut(s) 1142, 1177
XcmI CCANNNNNNNNNTGG 2 cut(s) 508, 1392
XhoI CTCGAG 1 cut(s) 705
XmiI GTMKAC 1 cut(s) 115
XspI CTAG 6 cut(s) 312, 761, 806, 1130, 1305, 1537
Zsp2I ATGCAT 2 cut(s) 17, 1321
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.