RLG00000027564

Tryptophan/tyrosine permease family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0013714
Physical Location & Seq
Forward (+)
12771517 .. 12774246
2730 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000027564

Sequence Viewer

Length: 1494 bp
ATGAACTTTGATATCTCCGAACTTGGTGTAGCTTTTAGTGGGTGTTTCCATAATCCAAATGACGAGCTTCTAACTAAATGCTATCATGCTTGTTCCTATAGCCAACAACTGCAGCAGCAGCAGCAAACTTCAACGTTTACTCGAAATGGTTTTGTCAAAAAGGCAAAGACAGGTGTTATACGGTTGAGCTTGGATGTAAAGCCCATCAAGGATCCCAATAAGAAGAAAGGAACTATTGCAGGGGCAGTTGCTTTGATAATTGGTACTAGTATAGGCTCAGGGATTCTTGCACTCCCAGAGAAAGCTTCTCCAGCGGGATTTATTCCAAGTTCAATATCGATAGTAATATGTTGGGGATTTCTCCTGATTGAGGCTCTTTTACTGATTGAAATCAATGTAAGCATGAGGAGGAGGAAAGGAAAGGAAGACAAAGAGAATGAGTTGGAGATCATTTCAATTAGGACTATGGCCCAAGAGACTCTAGGAGACTGGGGTGGAACTCTAGCCTCTCTGGTTTACATTTTCTTAGGTTATACTTCCATGATTTCTTACAGTTCCAAGTCTGGGGAGATCCTTTTTCACTTGATCAATCTCCCTGCTCCAGTTTCTGGCATCTTTTTCACTTCAGTTTTCACTCTTCTCATTTCCATCGGTGGGACTCAAGCTACGGATCAAGTTAACCAACTGCTCACTGCTTCCATGATAGGTTTACTAGTAGCAATAGAGGTGCTAGCAGTTGTGTTTGGAGGTTGGTCAGGACTACAGGGAAGTGGAGATTGGGGAAAAGTCCCACCTGCAATACCTGTCATAATATTTTCTTTGGTGTATCATGATCTAGCACCTGTTCTTTGTGCATATTTGGGCAGTGATCTCAAACGCTTAAGGATTTCGGTGGTTCTTGGTAGTCTTGTTCCATTGCTAGGACTACTTGTTTGGGATGCAATTGCTCTTGGCCTATCTGCCCAAGCTGACCAAATGGTTGACCCTGTTGAATTGCTTATGAGGGTGAAATGGAGTGGGGTTTCATATATGGTAGAAGCCTTTTCTCTGCTTGCAGTTGGAACATCACTAATTGGCACTCTTCTTGGCTTCTCTGAGTTTTTCAAAGAACAACTAAATAGCCTCTCATCGAATTCTCCCCTCACACAAACACAGAAACCAAACATGCCCTTTGGTCTGAGGAAATGGTGGGCAGGCAATACAGTTAGCTTCACATCAGTGGCATTGGTTGTTGTTCCATCTCTTTTTGTGTCGACTACTGTTCCGGATGCATTCTCTACTGCCACAGACGTTGCTGGAGGGTATTGTATGACAATGCTATATGGAGTTCTTCCACCAGCAATGGCCTGGGTAATGCATAATAAAGAAAGAGACAACGCTTCTGATCAAAAGACTTTATCGAGAGCGAGGCCAGTACTGGTGGCGGTAGGACTCCTTGCTTGCGGAATTGTGGCAGAGCAAATCCTTCAGGACATCTTCATATTTCAATTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Trp_Tyr_perm

Protein Analysis

498

Amino Acids

53.62

Weight (kDa)

6.54

Isoelectric Point (pI)

39.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aa_trans PF01490 76 - 256 1.2e-07 Transmembrane amino acid transporter protein
Trp_Tyr_perm PF03222 78 - 476 6.1e-47 Tryptophan/tyrosine permease family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013714)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g19650 FvH4_7g19650
malus_domestica MD07G1175000.v1.1
prunus_persica Prupe.2G214200_v2.0.a1 Prupe.2G214200_v2.0.a1 Prupe.2G214200_v2.0.a1
pyrus_communis pycom07g16940
rosa_chinensis RchiOBHm_Chr1g0363461
rosa_laevigata RLG00000027564
rosa_multiflora Rmu_sc0004458.1_g000002
rosa_roxburghii Rroxscaffold_4G00293160
rosa_rugosa Rorug01G0306900 Rorug01G0307000
rosa_samantha Rh1AG316100 Rh1BG279400 Rh1CG295700 Rh1DG310500
rosa_wichuraiana Rw1G027990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 802
Acc36I ACCTGC 1 cut(s) 802
AccB7I CCANNNNNTGG 1 cut(s) 608
AccI GTMKAC 1 cut(s) 1253
AccIII TCCGGA 1 cut(s) 1264
AciI CCGC 3 cut(s) 314, 1424, 1443
AclI AACGTT 1 cut(s) 134
AclWI GGATC 4 cut(s) 206, 219, 565, 678
AcsI RAATTY 1 cut(s) 1132
AcuI CTGAAG 2 cut(s) 609, 1451
AfaI GTAC 2 cut(s) 265, 1416
AfiI CCNNNNNNNGG 5 cut(s) 370, 564, 608, 654, 920
AflII CTTAAG 1 cut(s) 880
AgsI TTSAA 7 cut(s) 132, 333, 389, 456, 992, 1105, 1487
AhlI ACTAGT 2 cut(s) 266, 712
AjnI CCWGG 1 cut(s) 1346
AjuI GAANNNNNNNTTGG 2 cut(s) 760, 792
AleI CACNNNNGTG 1 cut(s) 1217
AluBI AGCT 7 cut(s) 32, 67, 189, 305, 665, 968, 1209
AluI AGCT 7 cut(s) 32, 67, 189, 305, 665, 968, 1209
Alw26I GTCTC 3 cut(s) 470, 480, 1365
AlwI GGATC 4 cut(s) 206, 219, 565, 678
AlwNI CAGNNNCTG 1 cut(s) 608
Aor13HI TCCGGA 1 cut(s) 1264
AoxI GGCC 4 cut(s) 468, 952, 1344, 1409
ApeKI GCWGC 4 cut(s) 112, 115, 118, 121
ApoI RAATTY 1 cut(s) 1132
ArsI GACNNNNNNTTYG 2 cut(s) 915, 947
AspS9I GGNCC 1 cut(s) 469
AsuHPI GGTGA 1 cut(s) 1018
AsuNHI GCTAGC 1 cut(s) 730
BamHI GGATCC 1 cut(s) 211
BbsI GAAGAC 1 cut(s) 432
BbvI GCAGC 4 cut(s) 124, 127, 130, 133
BccI CCATC 3 cut(s) 212, 656, 1246
BciT130I CCWGG 1 cut(s) 1348
BclI TGATCA 2 cut(s) 585, 1384
BcoDI GTCTC 3 cut(s) 470, 480, 1365
BcuI ACTAGT 2 cut(s) 266, 712
BfaI CTAG 8 cut(s) 267, 482, 503, 713, 731, 836, 920, 1492
BfmI CTRYAG 3 cut(s) 97, 110, 761
BfrI CTTAAG 1 cut(s) 880
BfuAI ACCTGC 1 cut(s) 802
BisI GCNGC 4 cut(s) 113, 116, 119, 122
BlsI GCNGC 4 cut(s) 114, 117, 120, 123
BmcAI AGTACT 1 cut(s) 1416
Bme1390I CCNGG 1 cut(s) 1348
BmgT120I GGNCC 1 cut(s) 469
BmiI GGNNCC 1 cut(s) 213
BmrFI CCNGG 1 cut(s) 1348
BmrI ACTGGG 1 cut(s) 499
BmsI GCATC 3 cut(s) 621, 928, 1258
BmtI GCTAGC 1 cut(s) 734
BmuI ACTGGG 1 cut(s) 499
BpiI GAAGAC 1 cut(s) 432
BpmI CTGGAG 3 cut(s) 294, 585, 1317
Bpu10I CCTNAGC 1 cut(s) 277
BpuEI CTTGAG 1 cut(s) 645
Bsa29I ATCGAT 1 cut(s) 338
BsaBI GATNNNNATC 1 cut(s) 389
BsaJI CCNNGG 1 cut(s) 1347
BsaWI WCCGGW 1 cut(s) 1264
Bsc4I CCNNNNNNNGG 5 cut(s) 370, 564, 608, 654, 920
Bse1I ACTGG 4 cut(s) 494, 602, 1412, 1422
Bse3DI GCAATG 2 cut(s) 914, 1347
Bse8I GATNNNNATC 1 cut(s) 389
BseAI TCCGGA 1 cut(s) 1264
BseBI CCWGG 1 cut(s) 1348
BseCI ATCGAT 1 cut(s) 338
BseDI CCNNGG 1 cut(s) 1347
BseGI GGATG 3 cut(s) 199, 943, 1273
BseJI GATNNNNATC 1 cut(s) 389
BseLI CCNNNNNNNGG 5 cut(s) 370, 564, 608, 654, 920
BseMI GCAATG 2 cut(s) 914, 1347
BseMII CTCAG 3 cut(s) 291, 1086, 1169
BseNI ACTGG 4 cut(s) 494, 602, 1412, 1422
BseRI GAGGAG 2 cut(s) 421, 424
BseXI GCAGC 4 cut(s) 124, 127, 130, 133
BshFI GGCC 4 cut(s) 470, 954, 1346, 1411
BshVI ATCGAT 1 cut(s) 338
BsiSI CCGG 1 cut(s) 1265
BslFI GGGAC 2 cut(s) 670, 773
BslI CCNNNNNNNGG 5 cut(s) 370, 564, 608, 654, 920
BsmAI GTCTC 3 cut(s) 470, 480, 1365
BsmFI GGGAC 2 cut(s) 670, 773
BsmI GAATGC 1 cut(s) 1271
BsnI GGCC 4 cut(s) 470, 954, 1346, 1411
Bsp13I TCCGGA 1 cut(s) 1264
Bsp143I GATC 8 cut(s) 211, 447, 570, 585, 670, 832, 868, 1384
BspACI CCGC 3 cut(s) 314, 1424, 1443
BspANI GGCC 4 cut(s) 470, 954, 1346, 1411
BspCNI CTCAG 3 cut(s) 290, 1087, 1170
BspDI ATCGAT 1 cut(s) 338
BspEI TCCGGA 1 cut(s) 1264
BspHI TCATGA 1 cut(s) 829
BspLI GGNNCC 1 cut(s) 213
BspMAI CTGCAG 1 cut(s) 114
BspMI ACCTGC 1 cut(s) 802
BspOI GCTAGC 1 cut(s) 734
BspPI GGATC 4 cut(s) 206, 219, 565, 678
BspTI CTTAAG 1 cut(s) 880
BsrDI GCAATG 2 cut(s) 914, 1347
BsrI ACTGG 4 cut(s) 494, 602, 1412, 1422
BssECI CCNNGG 1 cut(s) 1347
BssMI GATC 8 cut(s) 211, 447, 570, 585, 670, 832, 868, 1384
Bst2UI CCWGG 1 cut(s) 1348
Bst4CI ACNGT 4 cut(s) 183, 554, 1204, 1261
Bst6I CTCTTC 2 cut(s) 642, 1086
BstAFI CTTAAG 1 cut(s) 880
BstC8I GCNNGC 4 cut(s) 732, 1053, 1195, 1441
BstDEI CTNAG 4 cut(s) 277, 526, 1095, 1178
BstENI CCTNNNNNAGG 1 cut(s) 368
BstF5I GGATG 3 cut(s) 199, 943, 1273
BstKTI GATC 8 cut(s) 214, 450, 573, 588, 673, 835, 871, 1387
BstMAI GTCTC 3 cut(s) 470, 480, 1365
BstMBI GATC 8 cut(s) 211, 447, 570, 585, 670, 832, 868, 1384
BstMWI GCNNNNNNNGC 3 cut(s) 118, 121, 311
BstNI CCWGG 1 cut(s) 1348
BstNSI RCATGY 1 cut(s) 1168
BstSCI CCNGG 1 cut(s) 1346
BstSFI CTRYAG 3 cut(s) 97, 110, 761
BstV1I GCAGC 4 cut(s) 124, 127, 130, 133
BstV2I GAAGAC 1 cut(s) 432
BstX2I RGATCY 2 cut(s) 211, 570
BstYI RGATCY 2 cut(s) 211, 570
Bsu15I ATCGAT 1 cut(s) 338
BsuRI GGCC 4 cut(s) 470, 954, 1346, 1411
BsuTUI ATCGAT 1 cut(s) 338
BtsCI GGATG 3 cut(s) 199, 943, 1273
BtsI GCAGTG 2 cut(s) 690, 871
BtsIMutI CAGTG 3 cut(s) 690, 871, 1224
BveI ACCTGC 1 cut(s) 802
Cac8I GCNNGC 4 cut(s) 732, 1053, 1195, 1441
CaiI CAGNNNCTG 1 cut(s) 608
CciI TCATGA 1 cut(s) 829
Cfr13I GGNCC 1 cut(s) 469
ClaI ATCGAT 1 cut(s) 338
Csp6I GTAC 2 cut(s) 264, 1415
CspCI CAANNNNNGTGG 2 cut(s) 1273, 1308
CviAII CATG 6 cut(s) 86, 403, 541, 700, 830, 1165
CviQI GTAC 2 cut(s) 264, 1415
DdeI CTNAG 4 cut(s) 277, 526, 1095, 1178
DpnI GATC 8 cut(s) 213, 449, 572, 587, 672, 834, 870, 1386
DpnII GATC 8 cut(s) 211, 447, 570, 585, 670, 832, 868, 1384
Eam1104I CTCTTC 2 cut(s) 642, 1086
EarI CTCTTC 2 cut(s) 642, 1086
Eco32I GATATC 1 cut(s) 13
Eco57I CTGAAG 2 cut(s) 609, 1451
EcoNI CCTNNNNNAGG 1 cut(s) 368
EcoRI GAATTC 1 cut(s) 1132
EcoRII CCWGG 1 cut(s) 1346
EcoRV GATATC 1 cut(s) 13
EcoT22I ATGCAT 2 cut(s) 1273, 1359
FaeI CATG 6 cut(s) 89, 406, 544, 703, 833, 1168
FaqI GGGAC 2 cut(s) 670, 773
FatI CATG 6 cut(s) 85, 402, 540, 699, 829, 1164
FauI CCCGC 1 cut(s) 307
FbaI TGATCA 2 cut(s) 585, 1384
FblI GTMKAC 1 cut(s) 1253
Fnu4HI GCNGC 4 cut(s) 113, 116, 119, 122
FokI GGATG 3 cut(s) 206, 950, 1280
Fsp4HI GCNGC 4 cut(s) 113, 116, 119, 122
FspBI CTAG 8 cut(s) 267, 482, 503, 713, 731, 836, 920, 1492
GluI GCNGC 4 cut(s) 113, 116, 119, 122
GsuI CTGGAG 3 cut(s) 294, 585, 1317
HaeIII GGCC 4 cut(s) 470, 954, 1346, 1411
HapII CCGG 1 cut(s) 1265
Hin1II CATG 6 cut(s) 89, 406, 544, 703, 833, 1168
HincII GTYRAC 3 cut(s) 679, 982, 1254
HindII GTYRAC 3 cut(s) 679, 982, 1254
HindIII AAGCTT 1 cut(s) 303
HinfI GANTC 4 cut(s) 283, 478, 658, 1431
HpaI GTTAAC 1 cut(s) 679
HpaII CCGG 1 cut(s) 1265
HphI GGTGA 1 cut(s) 1018
Hpy166II GTNNAC 6 cut(s) 138, 517, 679, 710, 982, 1254
Hpy188I TCNGA 4 cut(s) 19, 1096, 1179, 1384
Hpy188III TCNNGA 6 cut(s) 364, 756, 830, 1265, 1401, 1469
Hpy8I GTNNAC 6 cut(s) 138, 517, 679, 710, 982, 1254
HpyAV CCTTC 1 cut(s) 1475
HpyCH4III ACNGT 4 cut(s) 183, 554, 1204, 1261
HpyCH4IV ACGT 2 cut(s) 134, 1290
HpyCH4V TGCA 9 cut(s) 112, 239, 290, 797, 854, 941, 1055, 1271, 1357
HpyF10VI GCNNNNNNNGC 3 cut(s) 118, 121, 311
HpyF3I CTNAG 4 cut(s) 277, 526, 1095, 1178
HpySE526I ACGT 2 cut(s) 134, 1290
Hsp92II CATG 6 cut(s) 89, 406, 544, 703, 833, 1168
Kpn2I TCCGGA 1 cut(s) 1264
Ksp22I TGATCA 2 cut(s) 585, 1384
KspAI GTTAAC 1 cut(s) 679
Kzo9I GATC 8 cut(s) 211, 447, 570, 585, 670, 832, 868, 1384
LmnI GCTCC 1 cut(s) 604
Lsp1109I GCAGC 4 cut(s) 124, 127, 130, 133
LweI GCATC 3 cut(s) 621, 928, 1258
MaeI CTAG 8 cut(s) 267, 482, 503, 713, 731, 836, 920, 1492
MaeII ACGT 2 cut(s) 134, 1290
MalI GATC 8 cut(s) 213, 449, 572, 587, 672, 834, 870, 1386
MboI GATC 8 cut(s) 211, 447, 570, 585, 670, 832, 868, 1384
MboII GAAGA 6 cut(s) 235, 437, 629, 1073, 1322, 1468
MfeI CAATTG 1 cut(s) 942
MflI RGATCY 2 cut(s) 211, 570
MluCI AATT 8 cut(s) 258, 456, 942, 992, 1071, 1132, 1446, 1487
MlyI GAGTC 3 cut(s) 472, 652, 1425
MmeI TCCRAC 2 cut(s) 423, 1039
Mph1103I ATGCAT 2 cut(s) 1273, 1359
MroI TCCGGA 1 cut(s) 1264
MseI TTAA 2 cut(s) 678, 881
MslI CAYNNNNRTG 1 cut(s) 1217
MspA1I CMGCKG 1 cut(s) 314
MspCI CTTAAG 1 cut(s) 880
MspI CCGG 1 cut(s) 1265
MspR9I CCNGG 1 cut(s) 1348
MunI CAATTG 1 cut(s) 942
Mva1269I GAATGC 1 cut(s) 1271
MvaI CCWGG 1 cut(s) 1348
MwoI GCNNNNNNNGC 3 cut(s) 118, 121, 311
NdeII GATC 8 cut(s) 211, 447, 570, 585, 670, 832, 868, 1384
NheI GCTAGC 1 cut(s) 730
NlaIII CATG 6 cut(s) 89, 406, 544, 703, 833, 1168
NlaIV GGNNCC 1 cut(s) 213
NsiI ATGCAT 2 cut(s) 1273, 1359
NspI RCATGY 1 cut(s) 1168
OliI CACNNNNGTG 1 cut(s) 1217
PagI TCATGA 1 cut(s) 829
PaqCI CACCTGC 1 cut(s) 802
PctI GAATGC 1 cut(s) 1271
PfeI GAWTC 1 cut(s) 283
PflMI CCANNNNNTGG 1 cut(s) 608
PkrI GCNGC 4 cut(s) 114, 117, 120, 123
PleI GAGTC 3 cut(s) 472, 652, 1425
PpsI GAGTC 3 cut(s) 472, 652, 1425
Psp1406I AACGTT 1 cut(s) 134
Psp6I CCWGG 1 cut(s) 1346
PspGI CCWGG 1 cut(s) 1346
PspN4I GGNNCC 1 cut(s) 213
PspPI GGNCC 1 cut(s) 469
PstI CTGCAG 1 cut(s) 114
PstNI CAGNNNCTG 1 cut(s) 608
PsuI RGATCY 2 cut(s) 211, 570
RsaI GTAC 2 cut(s) 265, 1416
RsaNI GTAC 2 cut(s) 264, 1415
RseI CAYNNNNRTG 1 cut(s) 1217
SalI GTCGAC 1 cut(s) 1252
SaqAI TTAA 2 cut(s) 678, 881
SatI GCNGC 4 cut(s) 113, 116, 119, 122
Sau3AI GATC 8 cut(s) 211, 447, 570, 585, 670, 832, 868, 1384
Sau96I GGNCC 1 cut(s) 469
ScaI AGTACT 1 cut(s) 1416
SchI GAGTC 3 cut(s) 472, 652, 1425
ScrFI CCNGG 1 cut(s) 1348
SfaNI GCATC 3 cut(s) 621, 928, 1258
SfcI CTRYAG 3 cut(s) 97, 110, 761
SmiMI CAYNNNNRTG 1 cut(s) 1217
SmlI CTYRAG 2 cut(s) 660, 880
SmoI CTYRAG 2 cut(s) 660, 880
SpeI ACTAGT 2 cut(s) 266, 712
Sse9I AATT 8 cut(s) 258, 456, 942, 992, 1071, 1132, 1446, 1487
SsiI CCGC 3 cut(s) 314, 1424, 1443
SspI AATATT 1 cut(s) 813
SspMI CTAG 8 cut(s) 267, 482, 503, 713, 731, 836, 920, 1492
StyD4I CCNGG 1 cut(s) 1346
TaaI ACNGT 4 cut(s) 183, 554, 1204, 1261
TaiI ACGT 2 cut(s) 137, 1293
TaqI TCGA 5 cut(s) 142, 338, 1130, 1253, 1400
TasI AATT 8 cut(s) 258, 456, 942, 992, 1071, 1132, 1446, 1487
TatI WGTACW 1 cut(s) 1414
TfiI GAWTC 1 cut(s) 283
Tru1I TTAA 2 cut(s) 678, 881
Tru9I TTAA 2 cut(s) 678, 881
TscAI CASTG 3 cut(s) 697, 871, 1224
TseI GCWGC 4 cut(s) 112, 115, 118, 121
TspDTI ATGAA 3 cut(s) 17, 1014, 1468
TspGWI ACGGA 1 cut(s) 683
TspRI CASTG 3 cut(s) 697, 871, 1224
Van91I CCANNNNNTGG 1 cut(s) 608
Vha464I CTTAAG 1 cut(s) 880
XagI CCTNNNNNAGG 1 cut(s) 368
XapI RAATTY 1 cut(s) 1132
XceI RCATGY 1 cut(s) 1168
XcmI CCANNNNNNNNNTGG 1 cut(s) 1344
XmiI GTMKAC 1 cut(s) 1253
XspI CTAG 8 cut(s) 267, 482, 503, 713, 731, 836, 920, 1492
ZrmI AGTACT 1 cut(s) 1416
Zsp2I ATGCAT 2 cut(s) 1273, 1359
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.