Prupe.4G088000_v2.0.a1

Protein kinase PINOID-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0012958
Physical Location & Seq
Reverse (-)
4316520 .. 4319726
3207 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G088000.2

Sequence Viewer

Length: 1383 bp
ATGTTAGAGCGTGACTCGGACGGTGAAGCCGTGAACTCGAGCCACAGCTCAATGAGCAGCGAGAGCTGTAGCAGCTTCAGCCGCCTCTCCTTCGACGCTGCCGCCACCACCACCGCCGCCGACTTCGCAACCGAAACCTTGTGCCTCAAGCCCCACCGCTCCTCCGACTTCGCCTACTCAGCCATTCGCTCCAAGAAAACCGCCCTCACTTTCCGCGACTTCCGCCTCCTCCGCCGCATCGGCGCCGGCGACATCGGCACCGTCTACCTCTGCACCCTCAGAGGCACCGGTTCCAGTCCCGCCGATCAGGATCGCGAGTCTTCTTCGTCTTCCTCGTGCTTTTACGCCATGAAAGTCGTCGACAAGGAGGCTCTGGCGCTGAAGAAGAAGGTCCACAGAGCCGAGATGGAGAGAAAAATCCTCAAGATGCTCGACCACCCGTTCTTGCCTACGCTCTACGCCGAGTTCGAAGCCTCGCATTTCTCTTGCATCGTCATGGAGTACTGTTCCGGCGGCGACTTGCACTCTCTCCGCCATAAGCAGCCTAACAAGCGCTTCTCTCTCACCTCGGCTAGGTTTTATGCTGCGGAGGTTCTGGTGGCCTTGGAATACCTCCACATGCTGGGAATCATCTACAGGGACCTAAAACCCGAGAACGTGCTGGTCAGATCGGACGGTCACATCATGCTCTCCGACTTTGACCTCTCCCTCTGCTCAGACGCAATCCCAGCCGTTGAATCCCCCGACTCCTACTCTGACTCGGGGTCCCTATCCACTCCATCCTACACCCGCCCGCGGAAAACCACAACACTCACCCCATTCTCCTGCCTCTCGACCCGGCTCTTCCGCTCGCGCAAAGTCCAGACCCTCTCCCCGAACCGGCTTTTCGTCGCCGAACCGGTCGCCGCCAGGTCACGTTCCTTCGTCGGTACCCACGAGTACGTGTCCCCGGAAGTCGCCTCCGGCAGTTCCCACGGAAACGCCGTTGACTGGTGGGCTTTCGGGATCTTCCTCTACGAGTTGATCTACGGCCGCACGCCGTTCGCGGCTCCATCGAACGAGAACACCCTGCGCAACATCGTGAAAAAGCCCCTTGCGTTCCCGACCCCCACGCCCTCCAGCGCGCTCGAAGGCCACGCGCGTGACCTCATTTCCGGGTTCCTCAACAAGGACCCGGAGCGCCGGCTCGGGTCGAAGCGCGGAGCCGCTGACGTCAAGAAGCATCCGTTCTTTAAGGGCATCAACTTGGCGTTGGTACGGTCCCTCACGCCGCCGATGATCCCGGGCCTAATACGAAGGCAAAACACGACGCCGTCTTATCAGGCCAAGAAGACTGATCAAAATAGAAATACGAGGCAATCTACGGCGTTCGAGTACTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000902 GO:0000904 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008152 GO:0009605 GO:0009606 GO:0009628 GO:0009629 GO:0009630 GO:0009653 GO:0009719 GO:0009725 GO:0009733 GO:0009734 GO:0009755 GO:0009790 GO:0009791 GO:0009793 GO:0009888 GO:0009908 GO:0009914 GO:0009926 GO:0009958 GO:0009986 GO:0009987 GO:0010015 GO:0010033 GO:0010053 GO:0010054 GO:0010154 GO:0010817 GO:0016020 GO:0016043 GO:0016049 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0021700 GO:0022414 GO:0022622 GO:0023052 GO:0030154 GO:0032501 GO:0032502 GO:0032870 GO:0032879 GO:0032989 GO:0035556 GO:0036211 GO:0040007 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0048316 GO:0048364 GO:0048366 GO:0048367 GO:0048468 GO:0048469 GO:0048588 GO:0048589 GO:0048608 GO:0048731 GO:0048764 GO:0048765 GO:0048766 GO:0048767 GO:0048825 GO:0048827 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051049 GO:0051179 GO:0051234 GO:0051716 GO:0060560 GO:0060918 GO:0061458 GO:0065007 GO:0065008 GO:0070887 GO:0071310 GO:0071365 GO:0071495 GO:0071695 GO:0071704 GO:0071840 GO:0071944 GO:0080147 GO:0080167 GO:0090558 GO:0090567 GO:0090627 GO:0099402 GO:0140096 GO:1901564 GO:1905392 GO:2000012
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

461

Amino Acids

51.14

Weight (kDa)

9.31

Isoelectric Point (pI)

52.78

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012958)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G34650
fragaria_vesca FvH4_3g09660
malus_domestica MD00G1109900.v1.1 MD05G1276300.v1.1
prunus_persica Prupe.4G088000_v2.0.a1 Prupe.4G088000_v2.0.a1
pyrus_communis pycom05g25730 pycom10g21130
rosa_chinensis RchiOBHm_Chr5g0015331
rosa_laevigata RLG00000032193
rosa_multiflora Rmu_co8298181.1_g000001
rosa_roxburghii Rroxscaffold_1G00061120
rosa_rugosa Rorug05G0026700
rosa_samantha Rh5AG120200 Rh5BG119100 Rh5CG129600 Rh5DG118000
rosa_wichuraiana Rw5G010390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1215
Acc16I TGCGCA 1 cut(s) 1073
Acc65I GGTACC 1 cut(s) 929
AccB1I GGYRCC 4 cut(s) 242, 257, 284, 929
AccB7I CCANNNNNTGG 1 cut(s) 622
AccBSI CCGCTC 2 cut(s) 159, 849
AccI GTMKAC 2 cut(s) 264, 360
AccII CGCG 9 cut(s) 216, 315, 796, 853, 1046, 1124, 1139, 1141, 1200
AclWI GGATC 3 cut(s) 318, 1013, 1273
AcoI YGGCCR 1 cut(s) 1030
AcuI CTGAAG 2 cut(s) 61, 401
AcyI GRCGYC 3 cut(s) 243, 1212, 1310
AfaI GTAC 5 cut(s) 503, 931, 941, 1257, 1376
AfeI AGCGCT 1 cut(s) 554
AfiI CCNNNNNNNGG 6 cut(s) 573, 622, 795, 879, 990, 1168
AflIII ACRYGT 1 cut(s) 942
AgeI ACCGGT 2 cut(s) 287, 898
AgsI TTSAA 1 cut(s) 737
AhdI GACNNNNNGTC 1 cut(s) 763
AjnI CCWGG 1 cut(s) 908
AleI CACNNNNGTG 1 cut(s) 1140
AluBI AGCT 3 cut(s) 48, 66, 75
AluI AGCT 3 cut(s) 48, 66, 75
AlwI GGATC 3 cut(s) 318, 1013, 1273
Ama87I CYCGRG 5 cut(s) 37, 650, 760, 1187, 1282
Aor51HI AGCGCT 1 cut(s) 554
AoxI GGCC 5 cut(s) 600, 1030, 1132, 1285, 1323
ApeKI GCWGC 5 cut(s) 57, 72, 98, 541, 584
AsiGI ACCGGT 2 cut(s) 287, 898
Asp718I GGTACC 1 cut(s) 929
AspS9I GGNCC 6 cut(s) 391, 640, 765, 1171, 1260, 1285
AsuC2I CCSGG 6 cut(s) 838, 950, 1156, 1175, 1283, 1284
AsuHPI GGTGA 3 cut(s) 35, 556, 805
AsuII TTCGAA 1 cut(s) 468
AvaI CYCGRG 5 cut(s) 37, 650, 760, 1187, 1282
AvaII GGWCC 5 cut(s) 391, 640, 765, 1171, 1260
BanI GGYRCC 4 cut(s) 242, 257, 284, 929
BauI CACGAG 2 cut(s) 334, 935
BbsI GAAGAC 3 cut(s) 312, 321, 1337
BbvI GCAGC 5 cut(s) 69, 84, 85, 553, 571
BccI CCATC 3 cut(s) 400, 787, 1060
BceAI ACGGC 7 cut(s) 14, 716, 968, 1024, 1045, 1297, 1380
BcgI CGANNNNNNTGC 2 cut(s) 1024, 1058
BciT130I CCWGG 1 cut(s) 910
BclI TGATCA 1 cut(s) 1336
BcnI CCSGG 6 cut(s) 838, 950, 1156, 1175, 1283, 1284
BfaI CTAG 1 cut(s) 573
BfmI CTRYAG 2 cut(s) 67, 634
BfoI RGCGCY 4 cut(s) 246, 380, 556, 1183
BglI GCCNNNNNGGC 1 cut(s) 240
BmcAI AGTACT 2 cut(s) 503, 1376
Bme1390I CCNGG 7 cut(s) 838, 910, 950, 1156, 1175, 1283, 1284
Bme18I GGWCC 5 cut(s) 391, 640, 765, 1171, 1260
BmeRI GACNNNNNGTC 1 cut(s) 763
BmeT110I CYCGRG 5 cut(s) 37, 650, 760, 1187, 1282
BmgT120I GGNCC 6 cut(s) 391, 640, 765, 1171, 1260, 1285
BmrFI CCNGG 7 cut(s) 838, 910, 950, 1156, 1175, 1283, 1284
BmsI GCATC 5 cut(s) 246, 417, 498, 1231, 1248
BpiI GAAGAC 3 cut(s) 312, 321, 1337
BplI GAGNNNNNCTC 1 cut(s) 31
BpmI CTGGAG 1 cut(s) 1102
Bpu14I TTCGAA 1 cut(s) 468
BpuEI CTTGAG 2 cut(s) 131, 407
BpuMI CCSGG 6 cut(s) 838, 950, 1156, 1175, 1283, 1284
BsaAI YACGTR 1 cut(s) 943
BsaBI GATNNNNATC 1 cut(s) 309
BsaHI GRCGYC 3 cut(s) 243, 1212, 1310
BsaJI CCNNGG 6 cut(s) 567, 603, 794, 948, 973, 1282
BsaWI WCCGGW 2 cut(s) 287, 898
BsaXI ACNNNNNCTCC 2 cut(s) 146, 176
Bsc4I CCNNNNNNNGG 6 cut(s) 573, 622, 795, 879, 990, 1168
Bse118I RCCGGY 5 cut(s) 245, 287, 879, 898, 1182
Bse1I ACTGG 2 cut(s) 294, 995
Bse8I GATNNNNATC 1 cut(s) 309
BseBI CCWGG 1 cut(s) 910
BseDI CCNNGG 6 cut(s) 567, 603, 794, 948, 973, 1282
BseGI GGATG 2 cut(s) 779, 1222
BseJI GATNNNNATC 1 cut(s) 309
BseLI CCNNNNNNNGG 6 cut(s) 573, 622, 795, 879, 990, 1168
BseMII CTCAG 3 cut(s) 192, 292, 729
BseNI ACTGG 2 cut(s) 294, 995
BsePI GCGCGC 1 cut(s) 1122
BseRI GAGGAG 2 cut(s) 151, 218
BseX3I CGGCCG 1 cut(s) 1030
BseXI GCAGC 5 cut(s) 69, 84, 85, 553, 571
BseYI CCCAGC 2 cut(s) 622, 727
BsgI GTGCAG 1 cut(s) 256
Bsh1236I CGCG 9 cut(s) 216, 315, 796, 853, 1046, 1124, 1139, 1141, 1200
Bsh1285I CGRYCG 2 cut(s) 903, 1033
BshFI GGCC 5 cut(s) 602, 1032, 1134, 1287, 1325
BshNI GGYRCC 4 cut(s) 242, 257, 284, 929
BshTI ACCGGT 2 cut(s) 287, 898
BsiEI CGRYCG 2 cut(s) 903, 1033
BsiHKCI CYCGRG 5 cut(s) 37, 650, 760, 1187, 1282
BslFI GGGAC 5 cut(s) 282, 653, 751, 931, 1246
BslI CCNNNNNNNGG 6 cut(s) 573, 622, 795, 879, 990, 1168
BsmFI GGGAC 5 cut(s) 282, 653, 751, 931, 1246
BsnI GGCC 5 cut(s) 602, 1032, 1134, 1287, 1325
BsoBI CYCGRG 5 cut(s) 37, 650, 760, 1187, 1282
Bsp119I TTCGAA 1 cut(s) 468
Bsp143I GATC 7 cut(s) 304, 310, 668, 1005, 1023, 1278, 1336
Bsp68I TCGCGA 1 cut(s) 315
BspANI GGCC 5 cut(s) 602, 1032, 1134, 1287, 1325
BspCNI CTCAG 3 cut(s) 191, 291, 728
BspFNI CGCG 9 cut(s) 216, 315, 796, 853, 1046, 1124, 1139, 1141, 1200
BspPI GGATC 3 cut(s) 318, 1013, 1273
BspQI GCTCTTC 1 cut(s) 848
BspT104I TTCGAA 1 cut(s) 468
BspT107I GGYRCC 4 cut(s) 242, 257, 284, 929
BsrBI CCGCTC 2 cut(s) 159, 849
BsrFI RCCGGY 5 cut(s) 245, 287, 879, 898, 1182
BsrI ACTGG 2 cut(s) 294, 995
BssAI RCCGGY 5 cut(s) 245, 287, 879, 898, 1182
BssECI CCNNGG 6 cut(s) 567, 603, 794, 948, 973, 1282
BssHII GCGCGC 1 cut(s) 1122
BssMI GATC 7 cut(s) 304, 310, 668, 1005, 1023, 1278, 1336
BssNI GRCGYC 3 cut(s) 243, 1212, 1310
BssSI CACGAG 2 cut(s) 334, 935
BssT1I CCWWGG 1 cut(s) 603
Bst2BI CACGAG 2 cut(s) 334, 935
Bst2UI CCWGG 1 cut(s) 910
Bst4CI ACNGT 5 cut(s) 23, 262, 506, 677, 1260
Bst6I CTCTTC 1 cut(s) 848
BstACI GRCGYC 3 cut(s) 243, 1212, 1310
BstBAI YACGTR 1 cut(s) 943
BstBI TTCGAA 1 cut(s) 468
BstC8I GCNNGC 6 cut(s) 247, 794, 851, 1037, 1124, 1184
BstDEI CTNAG 3 cut(s) 178, 278, 715
BstDSI CCRYGG 2 cut(s) 794, 973
BstENI CCTNNNNNAGG 2 cut(s) 571, 1166
BstF5I GGATG 2 cut(s) 779, 1222
BstFNI CGCG 9 cut(s) 216, 315, 796, 853, 1046, 1124, 1139, 1141, 1200
BstH2I RGCGCY 4 cut(s) 246, 380, 556, 1183
BstKTI GATC 7 cut(s) 307, 313, 671, 1008, 1026, 1281, 1339
BstMBI GATC 7 cut(s) 304, 310, 668, 1005, 1023, 1278, 1336
BstMCI CGRYCG 2 cut(s) 903, 1033
BstNI CCWGG 1 cut(s) 910
BstNSI RCATGY 1 cut(s) 622
BstSCI CCNGG 7 cut(s) 836, 908, 948, 1154, 1173, 1281, 1282
BstSFI CTRYAG 2 cut(s) 67, 634
BstUI CGCG 9 cut(s) 216, 315, 796, 853, 1046, 1124, 1139, 1141, 1200
BstV1I GCAGC 5 cut(s) 69, 84, 85, 553, 571
BstV2I GAAGAC 3 cut(s) 312, 321, 1337
BstX2I RGATCY 1 cut(s) 1005
BstYI RGATCY 1 cut(s) 1005
BstZI CGGCCG 1 cut(s) 1030
BsuRI GGCC 5 cut(s) 602, 1032, 1134, 1287, 1325
BtgI CCRYGG 2 cut(s) 794, 973
BtsCI GGATG 2 cut(s) 779, 1222
BtuMI TCGCGA 1 cut(s) 315
Cac8I GCNNGC 6 cut(s) 247, 794, 851, 1037, 1124, 1184
Cfr10I RCCGGY 5 cut(s) 245, 287, 879, 898, 1182
Cfr13I GGNCC 6 cut(s) 391, 640, 765, 1171, 1260, 1285
Cfr42I CCGCGG 1 cut(s) 797
Cfr9I CCCGGG 1 cut(s) 1282
CseI GACGC 3 cut(s) 104, 728, 1318
Csp6I GTAC 5 cut(s) 502, 930, 940, 1256, 1375
CspAI ACCGGT 2 cut(s) 287, 898
CviAII CATG 4 cut(s) 349, 496, 619, 685
CviQI GTAC 5 cut(s) 502, 930, 940, 1256, 1375
DdeI CTNAG 3 cut(s) 178, 278, 715
DinI GGCGCC 1 cut(s) 244
DpnI GATC 7 cut(s) 306, 312, 670, 1007, 1025, 1280, 1338
DpnII GATC 7 cut(s) 304, 310, 668, 1005, 1023, 1278, 1336
DriI GACNNNNNGTC 1 cut(s) 763
EaeI YGGCCR 1 cut(s) 1030
EagI CGGCCG 1 cut(s) 1030
Eam1104I CTCTTC 1 cut(s) 848
Eam1105I GACNNNNNGTC 1 cut(s) 763
EarI CTCTTC 1 cut(s) 848
EciI GGCGGA 3 cut(s) 212, 221, 521
EclXI CGGCCG 1 cut(s) 1030
Eco130I CCWWGG 1 cut(s) 603
Eco47I GGWCC 5 cut(s) 391, 640, 765, 1171, 1260
Eco47III AGCGCT 1 cut(s) 554
Eco52I CGGCCG 1 cut(s) 1030
Eco57I CTGAAG 2 cut(s) 61, 401
Eco88I CYCGRG 5 cut(s) 37, 650, 760, 1187, 1282
EcoNI CCTNNNNNAGG 2 cut(s) 571, 1166
EcoO109I RGGNCCY 3 cut(s) 640, 765, 1171
EcoRII CCWGG 1 cut(s) 908
EcoT14I CCWWGG 1 cut(s) 603
EgeI GGCGCC 1 cut(s) 244
EheI GGCGCC 1 cut(s) 244
ErhI CCWWGG 1 cut(s) 603
FaeI CATG 4 cut(s) 352, 499, 622, 688
FaiI YATR 6 cut(s) 350, 497, 537, 582, 620, 686
FaqI GGGAC 5 cut(s) 282, 653, 751, 931, 1246
FatI CATG 4 cut(s) 348, 495, 618, 684
FauI CCCGC 3 cut(s) 307, 797, 801
FbaI TGATCA 1 cut(s) 1336
FblI GTMKAC 2 cut(s) 264, 360
FokI GGATG 2 cut(s) 766, 1209
FspBI CTAG 1 cut(s) 573
FspI TGCGCA 1 cut(s) 1073
GsaI CCCAGC 2 cut(s) 626, 731
GsuI CTGGAG 1 cut(s) 1102
HaeII RGCGCY 4 cut(s) 246, 380, 556, 1183
HaeIII GGCC 5 cut(s) 602, 1032, 1134, 1287, 1325
HgaI GACGC 3 cut(s) 104, 728, 1318
Hin1I GRCGYC 3 cut(s) 243, 1212, 1310
Hin1II CATG 4 cut(s) 352, 499, 622, 688
HincII GTYRAC 2 cut(s) 361, 988
HindII GTYRAC 2 cut(s) 361, 988
HinfI GANTC 6 cut(s) 14, 317, 627, 737, 746, 758
HphI GGTGA 3 cut(s) 35, 556, 805
Hpy166II GTNNAC 5 cut(s) 34, 265, 361, 394, 988
Hpy188I TCNGA 9 cut(s) 19, 166, 281, 668, 673, 694, 718, 757, 1382
Hpy188III TCNNGA 9 cut(s) 308, 314, 424, 832, 862, 1003, 1081, 1102, 1216
Hpy8I GTNNAC 5 cut(s) 34, 265, 361, 394, 988
Hpy99I CGWCG 5 cut(s) 98, 362, 893, 929, 1312
HpyAV CCTTC 5 cut(s) 100, 382, 931, 1124, 1290
HpyCH4III ACNGT 5 cut(s) 23, 262, 506, 677, 1260
HpyCH4IV ACGT 4 cut(s) 657, 916, 942, 1212
HpyCH4V TGCA 3 cut(s) 273, 489, 523
HpyF3I CTNAG 3 cut(s) 178, 278, 715
HpySE526I ACGT 4 cut(s) 657, 916, 942, 1212
Hsp92I GRCGYC 3 cut(s) 243, 1212, 1310
Hsp92II CATG 4 cut(s) 352, 499, 622, 688
KasI GGCGCC 1 cut(s) 242
KflI GGGWCCC 1 cut(s) 765
KpnI GGTACC 1 cut(s) 933
KroI GCCGGC 2 cut(s) 245, 1182
KroNI GCCGGC 2 cut(s) 247, 1184
Ksp22I TGATCA 1 cut(s) 1336
KspI CCGCGG 1 cut(s) 797
Kzo9I GATC 7 cut(s) 304, 310, 668, 1005, 1023, 1278, 1336
LguI GCTCTTC 1 cut(s) 848
LmnI GCTCC 5 cut(s) 164, 194, 1054, 1177, 1202
Lsp1109I GCAGC 5 cut(s) 69, 84, 85, 553, 571
LweI GCATC 5 cut(s) 246, 417, 498, 1231, 1248
MaeI CTAG 1 cut(s) 573
MaeII ACGT 4 cut(s) 657, 916, 942, 1212
MaeIII GTNAC 4 cut(s) 11, 677, 912, 1142
MalI GATC 7 cut(s) 306, 312, 670, 1007, 1025, 1280, 1338
MbiI CCGCTC 2 cut(s) 159, 849
MboI GATC 7 cut(s) 304, 310, 668, 1005, 1023, 1278, 1336
MboII GAAGA 8 cut(s) 312, 315, 321, 394, 397, 835, 1000, 1342
MflI RGATCY 1 cut(s) 1005
Mly113I GGCGCC 1 cut(s) 243
MlyI GAGTC 4 cut(s) 8, 326, 740, 752
MmeI TCCRAC 2 cut(s) 189, 717
MreI CGCCGGCG 1 cut(s) 245
MroNI GCCGGC 2 cut(s) 245, 1182
MseI TTAA 1 cut(s) 1233
MslI CAYNNNNRTG 2 cut(s) 494, 1140
MspA1I CMGCKG 2 cut(s) 796, 1208
MspR9I CCNGG 7 cut(s) 838, 910, 950, 1156, 1175, 1283, 1284
MvaI CCWGG 1 cut(s) 910
MvnI CGCG 9 cut(s) 216, 315, 796, 853, 1046, 1124, 1139, 1141, 1200
NaeI GCCGGC 2 cut(s) 247, 1184
NarI GGCGCC 1 cut(s) 243
NciI CCSGG 6 cut(s) 838, 950, 1156, 1175, 1283, 1284
NdeII GATC 7 cut(s) 304, 310, 668, 1005, 1023, 1278, 1336
NgoMIV GCCGGC 2 cut(s) 245, 1182
NlaIII CATG 4 cut(s) 352, 499, 622, 688
NmeAIII GCCGAG 3 cut(s) 427, 487, 548
NmuCI GTSAC 4 cut(s) 11, 677, 912, 1142
NruI TCGCGA 1 cut(s) 315
NsbI TGCGCA 1 cut(s) 1073
NspI RCATGY 1 cut(s) 622
NspV TTCGAA 1 cut(s) 468
OliI CACNNNNGTG 1 cut(s) 1140
PaeR7I CTCGAG 1 cut(s) 37
PauI GCGCGC 1 cut(s) 1122
PciSI GCTCTTC 1 cut(s) 848
PcsI WCGNNNNNNNCGW 6 cut(s) 27, 246, 332, 465, 933, 981
PdiI GCCGGC 2 cut(s) 247, 1184
PfeI GAWTC 2 cut(s) 627, 737
PflFI GACNNNGTC 1 cut(s) 1312
PflMI CCANNNNNTGG 1 cut(s) 622
PinAI ACCGGT 2 cut(s) 287, 898
PleI GAGTC 4 cut(s) 8, 325, 740, 752
PluTI GGCGCC 1 cut(s) 246
PpsI GAGTC 4 cut(s) 8, 325, 740, 752
Ppu21I YACGTR 1 cut(s) 943
PpuMI RGGWCCY 3 cut(s) 640, 765, 1171
Psp5II RGGWCCY 3 cut(s) 640, 765, 1171
Psp6I CCWGG 1 cut(s) 908
PspFI CCCAGC 2 cut(s) 622, 727
PspGI CCWGG 1 cut(s) 908
PspPI GGNCC 6 cut(s) 391, 640, 765, 1171, 1260, 1285
PspPPI RGGWCCY 3 cut(s) 640, 765, 1171
PspXI VCTCGAGB 1 cut(s) 37
PsuI RGATCY 1 cut(s) 1005
PsyI GACNNNGTC 1 cut(s) 1312
PteI GCGCGC 1 cut(s) 1122
RruI TCGCGA 1 cut(s) 315
RsaI GTAC 5 cut(s) 503, 931, 941, 1257, 1376
RsaNI GTAC 5 cut(s) 502, 930, 940, 1256, 1375
RseI CAYNNNNRTG 2 cut(s) 494, 1140
SacII CCGCGG 1 cut(s) 797
SalI GTCGAC 1 cut(s) 359
SapI GCTCTTC 1 cut(s) 848
SaqAI TTAA 1 cut(s) 1233
Sau3AI GATC 7 cut(s) 304, 310, 668, 1005, 1023, 1278, 1336
Sau96I GGNCC 6 cut(s) 391, 640, 765, 1171, 1260, 1285
ScaI AGTACT 2 cut(s) 503, 1376
SchI GAGTC 4 cut(s) 8, 326, 740, 752
ScrFI CCNGG 7 cut(s) 838, 910, 950, 1156, 1175, 1283, 1284
SfaNI GCATC 5 cut(s) 246, 417, 498, 1231, 1248
SfcI CTRYAG 2 cut(s) 67, 634
SfoI GGCGCC 1 cut(s) 244
Sfr274I CTCGAG 1 cut(s) 37
Sfr303I CCGCGG 1 cut(s) 797
SfuI TTCGAA 1 cut(s) 468
SgrAI CRCCGGYG 1 cut(s) 245
SgrBI CCGCGG 1 cut(s) 797
SinI GGWCC 5 cut(s) 391, 640, 765, 1171, 1260
SlaI CTCGAG 1 cut(s) 37
SmaI CCCGGG 1 cut(s) 1284
SmiMI CAYNNNNRTG 2 cut(s) 494, 1140
SmlI CTYRAG 3 cut(s) 37, 146, 422
SmoI CTYRAG 3 cut(s) 37, 146, 422
SspDI GGCGCC 1 cut(s) 242
SspMI CTAG 1 cut(s) 573
StyD4I CCNGG 7 cut(s) 836, 908, 948, 1154, 1173, 1281, 1282
StyI CCWWGG 1 cut(s) 603
TaaI ACNGT 5 cut(s) 23, 262, 506, 677, 1260
TaiI ACGT 4 cut(s) 660, 919, 945, 1215
TatI WGTACW 2 cut(s) 501, 1374
TfiI GAWTC 2 cut(s) 627, 737
Tru1I TTAA 1 cut(s) 1233
Tru9I TTAA 1 cut(s) 1233
TseFI GTSAC 4 cut(s) 11, 677, 912, 1142
TseI GCWGC 5 cut(s) 57, 72, 98, 541, 584
Tsp45I GTSAC 4 cut(s) 11, 677, 912, 1142
TspDTI ATGAA 1 cut(s) 365
TspGWI ACGGA 2 cut(s) 990, 1215
TspMI CCCGGG 1 cut(s) 1282
Tth111I GACNNNGTC 1 cut(s) 1312
Van91I CCANNNNNTGG 1 cut(s) 622
VpaK11BI GGWCC 5 cut(s) 391, 640, 765, 1171, 1260
XagI CCTNNNNNAGG 2 cut(s) 571, 1166
XceI RCATGY 1 cut(s) 622
XhoI CTCGAG 1 cut(s) 37
XmaI CCCGGG 1 cut(s) 1282
XmiI GTMKAC 2 cut(s) 264, 360
XspI CTAG 1 cut(s) 573
ZraI GACGTC 1 cut(s) 1213
ZrmI AGTACT 2 cut(s) 503, 1376
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.