pycom05g25730

Protein kinase PINOID-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0012958
Physical Location & Seq
Forward (+)
27076898 .. 27078512
1615 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g25730.2

Sequence Viewer

Length: 1383 bp
ATGTTAGAGCGTGATTCGGATGGTGAAGCAGTAAACTCGAGTCACAGTTCAATGAGCAGTGAAAGCTGCAGCAGCTTCAGCCGCCTCTCGTTCGACGCCGCCGCCGCCACCACCACCGCCTCCGACCTCGCGGCCGAGACACTAAGCCTCAAGCCTCACCGCTCCTCCGACTTCGCCTACTCCGCCATTCGCTCCAAAAAAACAGCCCTCACTTTCCGCGACTTCCATCTCCACCGACGCATCGGCGCCGGCGACATCGGCACCGTCTACCTCTGCACACTCAGAAGCAGCGACTCTTCAAGTCCGGCCGATCAGTATACCGAGTCTACGTCTTCCTCGTGCCTTTACGCCATGAAAGTAGTCGACAAGGATGCTCTTGCGATGAAGAAGAAGGTGCAGAGAGCCGAGATGGAGCGAAAGATCCTAAAAATGCTTGACCACCCGTTCTTGCCTACTCTCTACGCCGAGTTCGAAGCCTCGCATTTCTCTTGCATCGTCATGGAGTACTGCTCCGGCGGCGACCTCCACTCCCTCCGCCACAATCAGCCTCAAAAACGCTTCTCCCTCAACTCCGCAAGGTTCTACGCTGCGGAGGTCCTGGTGGCGTTGGAATACCTCCACATGCTGGGAATCATCTACAGGGACCTAAAACCCGAGAACGTGCTGGTCAGATCGGACGGTCACATCATGCTCTCCGACTTTGACCTCTCACTATGCTCGGACGCAATCCCTGCCGTTGAATCACCCGACTCGTATTCCGACTCCGCGTCCTCATCGACCCCATCGTACACCCGCCCGCAGCAGAGGGCAACTCTCACCCCCTTCTCCTGCCTCTCCAACCGGCTGTTCCGGTCGCGCAAGGTCCGGACCCTTTCCCCGAACCGGCTCTTCGTCGCCGAACCGGTGGCCGCCCGGTCGCAGTCCTTCGTTGGGACCCACGAGTACGTGACCCCCGAAGTCGCCTCCGGTCGGTCCCACGGAAACGCCGTCGACTGGTGGGCATTCGGCATCTTCCTCTACGAGTTGGTCTACGGCCGGACGCCGTTCGCGGCTCCATCGAACGAGACCACCCTGCGTAACATTGTGAAAAAACCTCTAACATTCCCAACCTCCACGCCCTCCAGCGCGCTGGAGGGCCACGCGCGGGACCTCATTTCCGGGTTTCTCAACAAGGACCCGGACCGGCGACTCGGGTCGAAGCGCGGAGCCGCTGACGTCAAGAAGCATCCGTTCTTCAAGGGCATCAACTTGGCGTTGGTACGGTCGCTCACGCCGCCGATGATCCCGGGTTCGATAAGAAGGCAAAACACGACGCCGTATTGCCAGGCCAAGATCGAGGATAGTCGAAATACGAGACAATCGACGGAGTTCGATTACTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000902 GO:0000904 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008152 GO:0009605 GO:0009606 GO:0009628 GO:0009629 GO:0009630 GO:0009653 GO:0009719 GO:0009725 GO:0009733 GO:0009734 GO:0009755 GO:0009790 GO:0009791 GO:0009793 GO:0009888 GO:0009908 GO:0009914 GO:0009926 GO:0009958 GO:0009986 GO:0009987 GO:0010015 GO:0010033 GO:0010053 GO:0010054 GO:0010154 GO:0010817 GO:0016020 GO:0016043 GO:0016049 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0021700 GO:0022414 GO:0022622 GO:0023052 GO:0030154 GO:0032501 GO:0032502 GO:0032870 GO:0032879 GO:0032989 GO:0035556 GO:0036211 GO:0040007 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0048316 GO:0048364 GO:0048366 GO:0048367 GO:0048468 GO:0048469 GO:0048588 GO:0048589 GO:0048608 GO:0048731 GO:0048764 GO:0048765 GO:0048766 GO:0048767 GO:0048825 GO:0048827 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051049 GO:0051179 GO:0051234 GO:0051716 GO:0060560 GO:0060918 GO:0061458 GO:0065007 GO:0065008 GO:0070887 GO:0071310 GO:0071365 GO:0071495 GO:0071695 GO:0071704 GO:0071840 GO:0071944 GO:0080147 GO:0080167 GO:0090558 GO:0090567 GO:0090627 GO:0099402 GO:0140096 GO:1901564 GO:1905392 GO:2000012
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

461

Amino Acids

51.16

Weight (kDa)

8.87

Isoelectric Point (pI)

55.27

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012958)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G34650
fragaria_vesca FvH4_3g09660
malus_domestica MD00G1109900.v1.1 MD05G1276300.v1.1
prunus_persica Prupe.4G088000_v2.0.a1 Prupe.4G088000_v2.0.a1
pyrus_communis pycom05g25730 pycom10g21130
rosa_chinensis RchiOBHm_Chr5g0015331
rosa_laevigata RLG00000032193
rosa_multiflora Rmu_co8298181.1_g000001
rosa_roxburghii Rroxscaffold_1G00061120
rosa_rugosa Rorug05G0026700
rosa_samantha Rh5AG120200 Rh5BG119100 Rh5CG129600 Rh5DG118000
rosa_wichuraiana Rw5G010390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1218
AccB1I GGYRCC 2 cut(s) 245, 260
AccB7I CCANNNNNTGG 1 cut(s) 625
AccBSI CCGCTC 1 cut(s) 162
AccI GTMKAC 6 cut(s) 267, 317, 326, 363, 990, 1029
AccII CGCG 9 cut(s) 131, 219, 767, 856, 1049, 1127, 1142, 1144, 1203
AccIII TCCGGA 1 cut(s) 864
AclWI GGATC 2 cut(s) 415, 1276
AcoI YGGCCR 4 cut(s) 132, 306, 906, 1033
AcuI CTGAAG 1 cut(s) 61
AcyI GRCGYC 5 cut(s) 96, 246, 1040, 1215, 1313
AfaI GTAC 4 cut(s) 506, 788, 944, 1260
AfiI CCNNNNNNNGG 6 cut(s) 625, 882, 930, 969, 993, 1144
AgeI ACCGGT 1 cut(s) 901
AgsI TTSAA 4 cut(s) 51, 300, 740, 1237
AhdI GACNNNNNGTC 1 cut(s) 766
AjnI CCWGG 2 cut(s) 597, 1323
AjuI GAANNNNNNNTTGG 2 cut(s) 830, 862
AluBI AGCT 2 cut(s) 66, 75
AluI AGCT 2 cut(s) 66, 75
Alw26I GTCTC 3 cut(s) 131, 1058, 1348
AlwI GGATC 2 cut(s) 415, 1276
Ama87I CYCGRG 4 cut(s) 37, 653, 1190, 1285
Aor13HI TCCGGA 1 cut(s) 864
AoxI GGCC 6 cut(s) 132, 306, 906, 1033, 1135, 1326
ApeKI GCWGC 6 cut(s) 66, 69, 72, 288, 587, 799
AsiGI ACCGGT 1 cut(s) 901
AspLEI GCGC 6 cut(s) 248, 858, 1127, 1129, 1144, 1203
AsuC2I CCSGG 5 cut(s) 913, 1159, 1178, 1286, 1287
AsuHPI GGTGA 4 cut(s) 35, 149, 735, 808
AsuII TTCGAA 1 cut(s) 471
AvaI CYCGRG 4 cut(s) 37, 653, 1190, 1285
AvaII GGWCC 9 cut(s) 595, 643, 862, 867, 933, 972, 1147, 1174, 1180
BanI GGYRCC 2 cut(s) 245, 260
BauI CACGAG 2 cut(s) 337, 938
BbsI GAAGAC 1 cut(s) 324
BbvI GCAGC 6 cut(s) 53, 81, 84, 300, 574, 811
BccI CCATC 5 cut(s) 14, 234, 403, 790, 1063
BceAI ACGGC 5 cut(s) 719, 971, 1027, 1048, 1300
BcgI CGANNNNNNTGC 4 cut(s) 18, 52, 353, 387
BciT130I CCWGG 2 cut(s) 599, 1325
BcnI CCSGG 5 cut(s) 913, 1159, 1178, 1286, 1287
BcoDI GTCTC 3 cut(s) 131, 1058, 1348
BfmI CTRYAG 2 cut(s) 67, 637
BfoI RGCGCY 1 cut(s) 249
BmcAI AGTACT 1 cut(s) 506
Bme1390I CCNGG 7 cut(s) 599, 913, 1159, 1178, 1286, 1287, 1325
Bme18I GGWCC 9 cut(s) 595, 643, 862, 867, 933, 972, 1147, 1174, 1180
BmeRI GACNNNNNGTC 1 cut(s) 766
BmeT110I CYCGRG 4 cut(s) 37, 653, 1190, 1285
BmrFI CCNGG 7 cut(s) 599, 913, 1159, 1178, 1286, 1287, 1325
BmsI GCATC 6 cut(s) 249, 361, 501, 1017, 1234, 1251
BoxI GACNNNNGTC 1 cut(s) 1192
BpiI GAAGAC 1 cut(s) 324
BplI GAGNNNNNCTC 4 cut(s) 494, 526, 796, 828
BpmI CTGGAG 2 cut(s) 1105, 1151
Bpu14I TTCGAA 1 cut(s) 471
BpuEI CTTGAG 1 cut(s) 134
BpuMI CCSGG 5 cut(s) 913, 1159, 1178, 1286, 1287
BsaAI YACGTR 1 cut(s) 946
BsaHI GRCGYC 5 cut(s) 96, 246, 1040, 1215, 1313
BsaI GGTCTC 1 cut(s) 1058
BsaJI CCNNGG 2 cut(s) 976, 1285
BsaWI WCCGGW 4 cut(s) 849, 864, 901, 965
BsaXI ACNNNNNCTCC 4 cut(s) 149, 179, 512, 542
Bsc4I CCNNNNNNNGG 6 cut(s) 625, 882, 930, 969, 993, 1144
Bse118I RCCGGY 5 cut(s) 248, 840, 882, 901, 1182
Bse1I ACTGG 1 cut(s) 998
BseAI TCCGGA 1 cut(s) 864
BseBI CCWGG 2 cut(s) 599, 1325
BseDI CCNNGG 2 cut(s) 976, 1285
BseGI GGATG 3 cut(s) 25, 376, 1225
BseLI CCNNNNNNNGG 6 cut(s) 625, 882, 930, 969, 993, 1144
BseMII CTCAG 1 cut(s) 295
BseNI ACTGG 1 cut(s) 998
BsePI GCGCGC 1 cut(s) 1125
BseRI GAGGAG 1 cut(s) 154
BseX3I CGGCCG 3 cut(s) 132, 306, 1033
BseXI GCAGC 6 cut(s) 53, 81, 84, 300, 574, 811
BseYI CCCAGC 1 cut(s) 625
BsgI GTGCAG 2 cut(s) 259, 416
Bsh1236I CGCG 9 cut(s) 131, 219, 767, 856, 1049, 1127, 1142, 1144, 1203
Bsh1285I CGRYCG 7 cut(s) 135, 309, 854, 917, 970, 1036, 1265
BshFI GGCC 6 cut(s) 134, 308, 908, 1035, 1137, 1328
BshNI GGYRCC 2 cut(s) 245, 260
BshTI ACCGGT 1 cut(s) 901
BsiEI CGRYCG 7 cut(s) 135, 309, 854, 917, 970, 1036, 1265
BsiHKCI CYCGRG 4 cut(s) 37, 653, 1190, 1285
BslFI GGGAC 4 cut(s) 656, 946, 958, 1160
BslI CCNNNNNNNGG 6 cut(s) 625, 882, 930, 969, 993, 1144
BsmAI GTCTC 3 cut(s) 131, 1058, 1348
BsmFI GGGAC 4 cut(s) 656, 946, 958, 1160
BsmI GAATGC 1 cut(s) 1001
BsnI GGCC 6 cut(s) 134, 308, 908, 1035, 1137, 1328
Bso31I GGTCTC 1 cut(s) 1058
BsoBI CYCGRG 4 cut(s) 37, 653, 1190, 1285
Bsp119I TTCGAA 1 cut(s) 471
Bsp13I TCCGGA 1 cut(s) 864
Bsp143I GATC 5 cut(s) 310, 420, 671, 1281, 1332
BspANI GGCC 6 cut(s) 134, 308, 908, 1035, 1137, 1328
BspCNI CTCAG 1 cut(s) 294
BspEI TCCGGA 1 cut(s) 864
BspFNI CGCG 9 cut(s) 131, 219, 767, 856, 1049, 1127, 1142, 1144, 1203
BspMAI CTGCAG 1 cut(s) 71
BspPI GGATC 2 cut(s) 415, 1276
BspQI GCTCTTC 1 cut(s) 893
BspT104I TTCGAA 1 cut(s) 471
BspT107I GGYRCC 2 cut(s) 245, 260
BspTNI GGTCTC 1 cut(s) 1058
BsrBI CCGCTC 1 cut(s) 162
BsrFI RCCGGY 5 cut(s) 248, 840, 882, 901, 1182
BsrI ACTGG 1 cut(s) 998
BssAI RCCGGY 5 cut(s) 248, 840, 882, 901, 1182
BssECI CCNNGG 2 cut(s) 976, 1285
BssHII GCGCGC 1 cut(s) 1125
BssMI GATC 5 cut(s) 310, 420, 671, 1281, 1332
BssNAI GTATAC 1 cut(s) 318
BssNI GRCGYC 5 cut(s) 96, 246, 1040, 1215, 1313
BssSI CACGAG 2 cut(s) 337, 938
Bst1107I GTATAC 1 cut(s) 318
Bst2BI CACGAG 2 cut(s) 337, 938
Bst2UI CCWGG 2 cut(s) 599, 1325
Bst4CI ACNGT 4 cut(s) 47, 265, 680, 1263
Bst6I CTCTTC 2 cut(s) 301, 893
BstACI GRCGYC 5 cut(s) 96, 246, 1040, 1215, 1313
BstAPI GCANNNNNTGC 1 cut(s) 731
BstBAI YACGTR 1 cut(s) 946
BstBI TTCGAA 1 cut(s) 471
BstC8I GCNNGC 3 cut(s) 250, 797, 1127
BstDEI CTNAG 2 cut(s) 143, 281
BstDSI CCRYGG 1 cut(s) 976
BstF5I GGATG 3 cut(s) 25, 376, 1225
BstFNI CGCG 9 cut(s) 131, 219, 767, 856, 1049, 1127, 1142, 1144, 1203
BstH2I RGCGCY 1 cut(s) 249
BstHHI GCGC 6 cut(s) 248, 858, 1127, 1129, 1144, 1203
BstKTI GATC 5 cut(s) 313, 423, 674, 1284, 1335
BstMAI GTCTC 3 cut(s) 131, 1058, 1348
BstMBI GATC 5 cut(s) 310, 420, 671, 1281, 1332
BstMCI CGRYCG 7 cut(s) 135, 309, 854, 917, 970, 1036, 1265
BstNI CCWGG 2 cut(s) 599, 1325
BstNSI RCATGY 1 cut(s) 625
BstPAI GACNNNNGTC 1 cut(s) 1192
BstSCI CCNGG 7 cut(s) 597, 911, 1157, 1176, 1284, 1285, 1323
BstSFI CTRYAG 2 cut(s) 67, 637
BstUI CGCG 9 cut(s) 131, 219, 767, 856, 1049, 1127, 1142, 1144, 1203
BstV1I GCAGC 6 cut(s) 53, 81, 84, 300, 574, 811
BstV2I GAAGAC 1 cut(s) 324
BstX2I RGATCY 1 cut(s) 420
BstXI CCANNNNNNTGG 1 cut(s) 1129
BstYI RGATCY 1 cut(s) 420
BstZ17I GTATAC 1 cut(s) 318
BstZI CGGCCG 3 cut(s) 132, 306, 1033
BsuRI GGCC 6 cut(s) 134, 308, 908, 1035, 1137, 1328
BtgI CCRYGG 1 cut(s) 976
BtgZI GCGATG 1 cut(s) 395
BtsCI GGATG 3 cut(s) 25, 376, 1225
BtsI GCAGTG 1 cut(s) 64
BtsIMutI CAGTG 1 cut(s) 64
Cac8I GCNNGC 3 cut(s) 250, 797, 1127
CfoI GCGC 6 cut(s) 248, 858, 1127, 1129, 1144, 1203
Cfr10I RCCGGY 5 cut(s) 248, 840, 882, 901, 1182
Cfr9I CCCGGG 1 cut(s) 1285
CpoI CGGWCCG 1 cut(s) 1180
CseI GACGC 6 cut(s) 104, 246, 731, 756, 1048, 1321
Csp6I GTAC 4 cut(s) 505, 787, 943, 1259
CspAI ACCGGT 1 cut(s) 901
CspI CGGWCCG 1 cut(s) 1180
CviAII CATG 4 cut(s) 352, 499, 622, 688
CviQI GTAC 4 cut(s) 505, 787, 943, 1259
DdeI CTNAG 2 cut(s) 143, 281
DinI GGCGCC 1 cut(s) 247
DpnI GATC 5 cut(s) 312, 422, 673, 1283, 1334
DpnII GATC 5 cut(s) 310, 420, 671, 1281, 1332
DriI GACNNNNNGTC 1 cut(s) 766
EaeI YGGCCR 4 cut(s) 132, 306, 906, 1033
EagI CGGCCG 3 cut(s) 132, 306, 1033
Eam1104I CTCTTC 2 cut(s) 301, 893
Eam1105I GACNNNNNGTC 1 cut(s) 766
EarI CTCTTC 2 cut(s) 301, 893
EciI GGCGGA 2 cut(s) 172, 524
EclXI CGGCCG 3 cut(s) 132, 306, 1033
Eco31I GGTCTC 1 cut(s) 1058
Eco47I GGWCC 9 cut(s) 595, 643, 862, 867, 933, 972, 1147, 1174, 1180
Eco52I CGGCCG 3 cut(s) 132, 306, 1033
Eco57I CTGAAG 1 cut(s) 61
Eco88I CYCGRG 4 cut(s) 37, 653, 1190, 1285
EcoO109I RGGNCCY 5 cut(s) 595, 643, 933, 1147, 1174
EcoRII CCWGG 2 cut(s) 597, 1323
EgeI GGCGCC 1 cut(s) 247
EheI GGCGCC 1 cut(s) 247
FaeI CATG 4 cut(s) 355, 502, 625, 691
FaiI YATR 6 cut(s) 318, 353, 500, 623, 689, 715
FaqI GGGAC 4 cut(s) 656, 946, 958, 1160
FatI CATG 4 cut(s) 351, 498, 621, 687
FauI CCCGC 3 cut(s) 800, 804, 1137
FblI GTMKAC 6 cut(s) 267, 317, 326, 363, 990, 1029
FokI GGATG 3 cut(s) 32, 383, 1212
GlaI GCGC 6 cut(s) 247, 857, 1126, 1128, 1143, 1202
GsaI CCCAGC 1 cut(s) 629
GsuI CTGGAG 2 cut(s) 1105, 1151
HaeII RGCGCY 1 cut(s) 249
HaeIII GGCC 6 cut(s) 134, 308, 908, 1035, 1137, 1328
HgaI GACGC 6 cut(s) 104, 246, 731, 756, 1048, 1321
HhaI GCGC 6 cut(s) 248, 858, 1127, 1129, 1144, 1203
Hin1I GRCGYC 5 cut(s) 96, 246, 1040, 1215, 1313
Hin1II CATG 4 cut(s) 355, 502, 625, 691
Hin6I GCGC 6 cut(s) 246, 856, 1125, 1127, 1142, 1201
HinP1I GCGC 6 cut(s) 246, 856, 1125, 1127, 1142, 1201
HincII GTYRAC 2 cut(s) 364, 991
HindII GTYRAC 2 cut(s) 364, 991
HinfI GANTC 9 cut(s) 14, 40, 293, 323, 630, 740, 749, 761, 1188
HphI GGTGA 4 cut(s) 35, 149, 735, 808
Hpy166II GTNNAC 8 cut(s) 34, 268, 318, 327, 364, 789, 991, 1030
Hpy188III TCNNGA 2 cut(s) 865, 1219
Hpy8I GTNNAC 8 cut(s) 34, 268, 318, 327, 364, 789, 991, 1030
Hpy99I CGWCG 6 cut(s) 98, 240, 896, 992, 1315, 1366
HpyAV CCTTC 4 cut(s) 385, 832, 934, 1293
HpyCH4III ACNGT 4 cut(s) 47, 265, 680, 1263
HpyCH4IV ACGT 4 cut(s) 329, 660, 945, 1215
HpyCH4V TGCA 4 cut(s) 69, 276, 397, 492
HpyF3I CTNAG 2 cut(s) 143, 281
HpySE526I ACGT 4 cut(s) 329, 660, 945, 1215
Hsp92I GRCGYC 5 cut(s) 96, 246, 1040, 1215, 1313
Hsp92II CATG 4 cut(s) 355, 502, 625, 691
HspAI GCGC 6 cut(s) 246, 856, 1125, 1127, 1142, 1201
KasI GGCGCC 1 cut(s) 245
KflI GGGWCCC 1 cut(s) 933
Kpn2I TCCGGA 1 cut(s) 864
KroI GCCGGC 1 cut(s) 248
KroNI GCCGGC 1 cut(s) 250
Kzo9I GATC 5 cut(s) 310, 420, 671, 1281, 1332
LguI GCTCTTC 1 cut(s) 893
LmnI GCTCC 6 cut(s) 167, 197, 412, 515, 1057, 1205
Lsp1109I GCAGC 6 cut(s) 53, 81, 84, 300, 574, 811
LweI GCATC 6 cut(s) 249, 361, 501, 1017, 1234, 1251
MaeII ACGT 4 cut(s) 329, 660, 945, 1215
MaeIII GTNAC 4 cut(s) 41, 680, 946, 1076
MalI GATC 5 cut(s) 312, 422, 673, 1283, 1334
MbiI CCGCTC 1 cut(s) 162
MboI GATC 5 cut(s) 310, 420, 671, 1281, 1332
MboII GAAGA 7 cut(s) 288, 324, 397, 400, 880, 1003, 1225
MflI RGATCY 1 cut(s) 420
Mly113I GGCGCC 1 cut(s) 246
MlyI GAGTC 6 cut(s) 49, 287, 332, 743, 755, 1182
MmeI TCCRAC 6 cut(s) 147, 192, 588, 720, 783, 861
MreI CGCCGGCG 1 cut(s) 248
MroI TCCGGA 1 cut(s) 864
MroNI GCCGGC 1 cut(s) 248
MslI CAYNNNNRTG 1 cut(s) 497
MspA1I CMGCKG 1 cut(s) 1211
MspR9I CCNGG 7 cut(s) 599, 913, 1159, 1178, 1286, 1287, 1325
Mva1269I GAATGC 1 cut(s) 1001
MvaI CCWGG 2 cut(s) 599, 1325
MvnI CGCG 9 cut(s) 131, 219, 767, 856, 1049, 1127, 1142, 1144, 1203
NaeI GCCGGC 1 cut(s) 250
NarI GGCGCC 1 cut(s) 246
NciI CCSGG 5 cut(s) 913, 1159, 1178, 1286, 1287
NdeII GATC 5 cut(s) 310, 420, 671, 1281, 1332
NgoMIV GCCGGC 1 cut(s) 248
NlaIII CATG 4 cut(s) 355, 502, 625, 691
NmeAIII GCCGAG 3 cut(s) 160, 430, 490
NmuCI GTSAC 3 cut(s) 41, 680, 946
NspI RCATGY 1 cut(s) 625
NspV TTCGAA 1 cut(s) 471
PaeR7I CTCGAG 1 cut(s) 37
PauI GCGCGC 1 cut(s) 1125
PciSI GCTCTTC 1 cut(s) 893
PcsI WCGNNNNNNNCGW 7 cut(s) 249, 335, 468, 782, 951, 984, 1358
PctI GAATGC 1 cut(s) 1001
PdiI GCCGGC 1 cut(s) 250
PfeI GAWTC 3 cut(s) 14, 630, 740
PflMI CCANNNNNTGG 1 cut(s) 625
PinAI ACCGGT 1 cut(s) 901
PleI GAGTC 6 cut(s) 48, 287, 331, 743, 755, 1182
PluTI GGCGCC 1 cut(s) 249
PpsI GAGTC 6 cut(s) 48, 287, 331, 743, 755, 1182
Ppu21I YACGTR 1 cut(s) 946
PpuMI RGGWCCY 5 cut(s) 595, 643, 933, 1147, 1174
PshAI GACNNNNGTC 1 cut(s) 1192
Psp5II RGGWCCY 5 cut(s) 595, 643, 933, 1147, 1174
Psp6I CCWGG 2 cut(s) 597, 1323
PspFI CCCAGC 1 cut(s) 625
PspGI CCWGG 2 cut(s) 597, 1323
PspPPI RGGWCCY 5 cut(s) 595, 643, 933, 1147, 1174
PspXI VCTCGAGB 1 cut(s) 37
PstI CTGCAG 1 cut(s) 71
PsuI RGATCY 1 cut(s) 420
PteI GCGCGC 1 cut(s) 1125
RsaI GTAC 4 cut(s) 506, 788, 944, 1260
RsaNI GTAC 4 cut(s) 505, 787, 943, 1259
RseI CAYNNNNRTG 1 cut(s) 497
Rsr2I CGGWCCG 1 cut(s) 1180
RsrII CGGWCCG 1 cut(s) 1180
SalI GTCGAC 2 cut(s) 362, 989
SapI GCTCTTC 1 cut(s) 893
Sau3AI GATC 5 cut(s) 310, 420, 671, 1281, 1332
ScaI AGTACT 1 cut(s) 506
SchI GAGTC 6 cut(s) 49, 287, 332, 743, 755, 1182
ScrFI CCNGG 7 cut(s) 599, 913, 1159, 1178, 1286, 1287, 1325
SfaNI GCATC 6 cut(s) 249, 361, 501, 1017, 1234, 1251
SfcI CTRYAG 2 cut(s) 67, 637
SfoI GGCGCC 1 cut(s) 247
Sfr274I CTCGAG 1 cut(s) 37
SfuI TTCGAA 1 cut(s) 471
SgrAI CRCCGGYG 1 cut(s) 248
SinI GGWCC 9 cut(s) 595, 643, 862, 867, 933, 972, 1147, 1174, 1180
SlaI CTCGAG 1 cut(s) 37
SmaI CCCGGG 1 cut(s) 1287
SmiMI CAYNNNNRTG 1 cut(s) 497
SmlI CTYRAG 2 cut(s) 37, 149
SmoI CTYRAG 2 cut(s) 37, 149
SspDI GGCGCC 1 cut(s) 245
StyD4I CCNGG 7 cut(s) 597, 911, 1157, 1176, 1284, 1285, 1323
TaaI ACNGT 4 cut(s) 47, 265, 680, 1263
TaiI ACGT 4 cut(s) 332, 663, 948, 1218
TaqII GACCGA 1 cut(s) 960
TatI WGTACW 1 cut(s) 504
TfiI GAWTC 3 cut(s) 14, 630, 740
TscAI CASTG 1 cut(s) 64
TseFI GTSAC 3 cut(s) 41, 680, 946
TseI GCWGC 6 cut(s) 66, 69, 72, 288, 587, 799
Tsp45I GTSAC 3 cut(s) 41, 680, 946
TspDTI ATGAA 2 cut(s) 368, 398
TspGWI ACGGA 3 cut(s) 993, 1218, 1379
TspMI CCCGGG 1 cut(s) 1285
TspRI CASTG 1 cut(s) 64
Van91I CCANNNNNTGG 1 cut(s) 625
VpaK11BI GGWCC 9 cut(s) 595, 643, 862, 867, 933, 972, 1147, 1174, 1180
XceI RCATGY 1 cut(s) 625
XhoI CTCGAG 1 cut(s) 37
XmaI CCCGGG 1 cut(s) 1285
XmiI GTMKAC 6 cut(s) 267, 317, 326, 363, 990, 1029
ZraI GACGTC 1 cut(s) 1216
ZrmI AGTACT 1 cut(s) 506
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.