Rroxscaffold_1G00061120

Protein kinase PINOID-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0012958
Physical Location & Seq
Forward (+)
83305984 .. 83308346
2363 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00061120.1

Sequence Viewer

Length: 1371 bp
ATGGTAGAGCTTGGTCCGATTAGTACTACTCTCGCGCGTGATTCAGACAGTTCAATGAGTAGTGACACCTGTAGCAGTTTTAGCCGCCTCTCCTTCGACGCAACCACCACCTTAACCGCCGCAACCGACTTCTTCGGCACCGAGAATCTCGCCCTCAAGCCGCACCGCTCCTCCGACTTCGCCTACTCCGTCATTCGCTCCAAGAAATCCGCCCTCACTTACCGCGACTTCCGCCTCCACCGCCGCATCGGCTCCGGCGACATCGGCACCGTCTACCTCTGCAGCCTCCGCAGTCCCGACTCCGACTCATCATCATCATCCTCAATCTCATGCGCCTACGCCATGAAAGTCGTCGACAAGGAGGCCTTGGCCCTCAAGAAGAAGGAGCCCAGAGCCGAGATGGAGCGCAAGATCCTCAAAATGCTTGACCACCCTTTCCTCCCGACTCTCTACGCCGAGTTCGAGGCCTCGCATTTCTCCTGCATCGTCATGGAGTACTGCTCCGGCGGCGACTTGCACTCCCTCCGCCATAAACAGCCCAACCGCCGCTTCTCTCTCAGCTCCGCAAGGTTCTACGCTGCTGAGGTCCTGGTGGCTTTGGAGTACCTCCACATGTTGGGAATAATCTACAGAGACCTGAAGCCGGAGAACGTGCTCGTCAGATCCGACGGTCACATCATGCTCTCCGACTTTGACCTCTCTCTCTGCTCCGACGCAATCCCCGCCGTTGAGTCACCCGACTCCTCTCCGGTTCTAGAGTCCATCTCAACTCCATCGTACGCCCGCCCCCACCCGAGCACTCTCACTCCCTTCTCCTGCCTCTCAAACCGGCTGTTCCGGTCCAGAAAGGTTCAGACTTTGTCGCCGAACCGCCTCTTCGTGGCTGAGCCGGTCGCCGCCCGTTCATGCTCGTTCGTCGGGACCCACGAGTACGTGGCGCCGGAGGTTGCTTCCGGTAGGTCCCACGGCAACGCGGTTGACTGGTGGGCGTTTGGGATCTTCCTCTACGAGATGATCTACGGGCGGACGCCGTTTGCGGCTCCATCGAACGAGACTACACTGCGAAGCATCGTGAAAAGCCCCTTAACCTTCCCGACCTCCACGCCCTGCAGCGCGCTTGAGGGCCACGCGCGGGACCTGATATCCGGGTTTCTGAACAAGGACCCGGAGCGGCGGCTCGGGTCGAAGCGCGGCGCTGCTGACGTCAAGAAGCATCCTTTCTTCAAGGGGATCAACTTGGCGTTGATACGGTCGCTGCGGCCGCCGATGGTCCCGGGTCTGAGAAGGCAGAACACGACGCCGTATTACCACGCCAAGTCTGATGATCAGAATAAAAATAACGGACAGTCTGGGGCGTTCGACTACTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000902 GO:0000904 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008152 GO:0009605 GO:0009606 GO:0009628 GO:0009629 GO:0009630 GO:0009653 GO:0009719 GO:0009725 GO:0009733 GO:0009734 GO:0009755 GO:0009790 GO:0009791 GO:0009793 GO:0009888 GO:0009908 GO:0009914 GO:0009926 GO:0009958 GO:0009986 GO:0009987 GO:0010015 GO:0010033 GO:0010053 GO:0010054 GO:0010154 GO:0010817 GO:0016020 GO:0016043 GO:0016049 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0021700 GO:0022414 GO:0022622 GO:0023052 GO:0030154 GO:0032501 GO:0032502 GO:0032870 GO:0032879 GO:0032989 GO:0035556 GO:0036211 GO:0040007 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0048316 GO:0048364 GO:0048366 GO:0048367 GO:0048468 GO:0048469 GO:0048588 GO:0048589 GO:0048608 GO:0048731 GO:0048764 GO:0048765 GO:0048766 GO:0048767 GO:0048825 GO:0048827 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051049 GO:0051179 GO:0051234 GO:0051716 GO:0060560 GO:0060918 GO:0061458 GO:0065007 GO:0065008 GO:0070887 GO:0071310 GO:0071365 GO:0071495 GO:0071695 GO:0071704 GO:0071840 GO:0071944 GO:0080147 GO:0080167 GO:0090558 GO:0090567 GO:0090627 GO:0099402 GO:0140096 GO:1901564 GO:1905392 GO:2000012
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

456

Amino Acids

50.61

Weight (kDa)

8.93

Isoelectric Point (pI)

54.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 78 - 234 2.2e-31 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 78 - 234 8.1e-20 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 302 - 408 2.5e-19 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 310 - 392 5.1e-06 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012958)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G34650
fragaria_vesca FvH4_3g09660
malus_domestica MD00G1109900.v1.1 MD05G1276300.v1.1
prunus_persica Prupe.4G088000_v2.0.a1 Prupe.4G088000_v2.0.a1
pyrus_communis pycom05g25730 pycom10g21130
rosa_chinensis RchiOBHm_Chr5g0015331
rosa_laevigata RLG00000032193
rosa_multiflora Rmu_co8298181.1_g000001
rosa_roxburghii Rroxscaffold_1G00061120
rosa_rugosa Rorug05G0026700
rosa_samantha Rh5AG120200 Rh5BG119100 Rh5CG129600 Rh5DG118000
rosa_wichuraiana Rw5G010390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1206
AccB1I GGYRCC 3 cut(s) 137, 266, 937
AccB7I CCANNNNNTGG 1 cut(s) 616
AccBSI CCGCTC 2 cut(s) 168, 1171
AccI GTMKAC 2 cut(s) 273, 354
AccII CGCG 8 cut(s) 35, 37, 225, 974, 1115, 1130, 1132, 1191
AclWI GGATC 4 cut(s) 406, 657, 1004, 1238
AcoI YGGCCR 1 cut(s) 1259
AcuI CTGAAG 1 cut(s) 659
AcyI GRCGYC 4 cut(s) 938, 1028, 1203, 1298
AfaI GTAC 5 cut(s) 25, 497, 605, 779, 932
AfiI CCNNNNNNNGG 4 cut(s) 616, 643, 880, 1132
AflIII ACRYGT 1 cut(s) 612
AgsI TTSAA 2 cut(s) 54, 1225
AjnI CCWGG 1 cut(s) 588
AjuI GAANNNNNNNTTGG 2 cut(s) 533, 565
AluBI AGCT 2 cut(s) 10, 561
AluI AGCT 2 cut(s) 10, 561
Alw21I GWGCWC 2 cut(s) 657, 800
Alw26I GTCTC 2 cut(s) 627, 1046
AlwI GGATC 4 cut(s) 406, 657, 1004, 1238
Ama87I CYCGRG 3 cut(s) 793, 1178, 1273
AoxI GGCC 5 cut(s) 363, 369, 465, 1123, 1259
ApeKI GCWGC 5 cut(s) 282, 578, 1110, 1196, 1255
AspLEI GCGC 9 cut(s) 37, 335, 408, 940, 1115, 1117, 1132, 1191, 1196
AsuC2I CCSGG 4 cut(s) 1147, 1166, 1274, 1275
AsuHPI GGTGA 1 cut(s) 726
AvaI CYCGRG 3 cut(s) 793, 1178, 1273
AvaII GGWCC 8 cut(s) 14, 586, 840, 921, 960, 1135, 1162, 1270
BanI GGYRCC 3 cut(s) 137, 266, 937
BanII GRGCYC 1 cut(s) 390
BauI CACGAG 1 cut(s) 926
Bbv12I GWGCWC 2 cut(s) 657, 800
BbvCI CCTCAGC 1 cut(s) 582
BbvI GCAGC 5 cut(s) 294, 565, 1122, 1183, 1242
BccI CCATC 5 cut(s) 394, 770, 781, 1051, 1261
BceAI ACGGC 4 cut(s) 710, 982, 1015, 1285
BciT130I CCWGG 1 cut(s) 590
BclI TGATCA 1 cut(s) 1324
BcnI CCSGG 4 cut(s) 1147, 1166, 1274, 1275
BcoDI GTCTC 2 cut(s) 627, 1046
BfaI CTAG 1 cut(s) 755
BfmI CTRYAG 4 cut(s) 70, 280, 628, 1108
BfoI RGCGCY 2 cut(s) 941, 1197
BglI GCCNNNNNGGC 1 cut(s) 249
BlpI GCTNAGC 1 cut(s) 885
BmcAI AGTACT 2 cut(s) 25, 497
Bme1390I CCNGG 5 cut(s) 590, 1147, 1166, 1274, 1275
Bme18I GGWCC 8 cut(s) 14, 586, 840, 921, 960, 1135, 1162, 1270
BmeT110I CYCGRG 3 cut(s) 793, 1178, 1273
BmrFI CCNGG 5 cut(s) 590, 1147, 1166, 1274, 1275
BmsI GCATC 4 cut(s) 255, 492, 1077, 1222
BplI GAGNNNNNCTC 4 cut(s) 485, 517, 749, 781
Bpu10I CCTNAGC 1 cut(s) 582
Bpu1102I GCTNAGC 1 cut(s) 885
BpuEI CTTGAG 3 cut(s) 140, 359, 1139
BpuMI CCSGG 4 cut(s) 1147, 1166, 1274, 1275
BsaAI YACGTR 1 cut(s) 934
BsaHI GRCGYC 4 cut(s) 938, 1028, 1203, 1298
BsaI GGTCTC 1 cut(s) 627
BsaJI CCNNGG 3 cut(s) 366, 964, 1273
BsaWI WCCGGW 3 cut(s) 748, 837, 953
BsaXI ACNNNNNCTCC 6 cut(s) 155, 185, 503, 533, 790, 820
Bsc4I CCNNNNNNNGG 4 cut(s) 616, 643, 880, 1132
Bse118I RCCGGY 2 cut(s) 828, 889
Bse1I ACTGG 1 cut(s) 986
BseBI CCWGG 1 cut(s) 590
BseDI CCNNGG 3 cut(s) 366, 964, 1273
BseGI GGATG 2 cut(s) 317, 1213
BseLI CCNNNNNNNGG 4 cut(s) 616, 643, 880, 1132
BseMII CTCAG 4 cut(s) 571, 573, 876, 1271
BseNI ACTGG 1 cut(s) 986
BsePI GCGCGC 1 cut(s) 1113
BseRI GAGGAG 2 cut(s) 160, 733
BseX3I CGGCCG 1 cut(s) 1259
BseXI GCAGC 5 cut(s) 294, 565, 1122, 1183, 1242
Bsh1236I CGCG 8 cut(s) 35, 37, 225, 974, 1115, 1130, 1132, 1191
Bsh1285I CGRYCG 3 cut(s) 894, 1253, 1262
BshFI GGCC 5 cut(s) 365, 371, 467, 1125, 1261
BshNI GGYRCC 3 cut(s) 137, 266, 937
BsiEI CGRYCG 3 cut(s) 894, 1253, 1262
BsiHKAI GWGCWC 2 cut(s) 657, 800
BsiHKCI CYCGRG 3 cut(s) 793, 1178, 1273
BsiWI CGTACG 1 cut(s) 777
BslFI GGGAC 5 cut(s) 279, 934, 946, 1148, 1256
BslI CCNNNNNNNGG 4 cut(s) 616, 643, 880, 1132
BsmAI GTCTC 2 cut(s) 627, 1046
BsmFI GGGAC 5 cut(s) 279, 934, 946, 1148, 1256
BsnI GGCC 5 cut(s) 365, 371, 467, 1125, 1261
Bso31I GGTCTC 1 cut(s) 627
BsoBI CYCGRG 3 cut(s) 793, 1178, 1273
Bsp1286I GDGCHC 3 cut(s) 390, 657, 800
Bsp143I GATC 6 cut(s) 411, 662, 996, 1014, 1230, 1324
Bsp1720I GCTNAGC 1 cut(s) 885
BspANI GGCC 5 cut(s) 365, 371, 467, 1125, 1261
BspCNI CTCAG 4 cut(s) 570, 574, 877, 1272
BspFNI CGCG 8 cut(s) 35, 37, 225, 974, 1115, 1130, 1132, 1191
BspMAI CTGCAG 2 cut(s) 284, 1112
BspPI GGATC 4 cut(s) 406, 657, 1004, 1238
BspT107I GGYRCC 3 cut(s) 137, 266, 937
BspTNI GGTCTC 1 cut(s) 627
BsrBI CCGCTC 2 cut(s) 168, 1171
BsrFI RCCGGY 2 cut(s) 828, 889
BsrI ACTGG 1 cut(s) 986
BssAI RCCGGY 2 cut(s) 828, 889
BssECI CCNNGG 3 cut(s) 366, 964, 1273
BssHII GCGCGC 1 cut(s) 1113
BssMI GATC 6 cut(s) 411, 662, 996, 1014, 1230, 1324
BssNI GRCGYC 4 cut(s) 938, 1028, 1203, 1298
BssSI CACGAG 1 cut(s) 926
BssT1I CCWWGG 1 cut(s) 366
Bst2BI CACGAG 1 cut(s) 926
Bst2UI CCWGG 1 cut(s) 590
Bst4CI ACNGT 5 cut(s) 50, 271, 671, 1251, 1347
Bst6I CTCTTC 1 cut(s) 881
BstACI GRCGYC 4 cut(s) 938, 1028, 1203, 1298
BstBAI YACGTR 1 cut(s) 934
BstC8I GCNNGC 2 cut(s) 784, 1115
BstDEI CTNAG 4 cut(s) 557, 582, 885, 1280
BstDSI CCRYGG 1 cut(s) 964
BstF5I GGATG 2 cut(s) 317, 1213
BstFNI CGCG 8 cut(s) 35, 37, 225, 974, 1115, 1130, 1132, 1191
BstH2I RGCGCY 2 cut(s) 941, 1197
BstHHI GCGC 9 cut(s) 37, 335, 408, 940, 1115, 1117, 1132, 1191, 1196
BstKTI GATC 6 cut(s) 414, 665, 999, 1017, 1233, 1327
BstMAI GTCTC 2 cut(s) 627, 1046
BstMBI GATC 6 cut(s) 411, 662, 996, 1014, 1230, 1324
BstMCI CGRYCG 3 cut(s) 894, 1253, 1262
BstMWI GCNNNNNNNGC 9 cut(s) 81, 231, 240, 249, 264, 288, 507, 722, 1261
BstNI CCWGG 1 cut(s) 590
BstNSI RCATGY 1 cut(s) 616
BstSCI CCNGG 5 cut(s) 588, 1145, 1164, 1272, 1273
BstSFI CTRYAG 4 cut(s) 70, 280, 628, 1108
BstUI CGCG 8 cut(s) 35, 37, 225, 974, 1115, 1130, 1132, 1191
BstV1I GCAGC 5 cut(s) 294, 565, 1122, 1183, 1242
BstX2I RGATCY 3 cut(s) 411, 662, 996
BstYI RGATCY 3 cut(s) 411, 662, 996
BstZI CGGCCG 1 cut(s) 1259
BsuRI GGCC 5 cut(s) 365, 371, 467, 1125, 1261
BtgI CCRYGG 1 cut(s) 964
BtsCI GGATG 2 cut(s) 317, 1213
BtsI GCAGTG 1 cut(s) 1058
BtsIMutI CAGTG 1 cut(s) 1058
Cac8I GCNNGC 2 cut(s) 784, 1115
CciNI GCGGCCGC 1 cut(s) 1259
CfoI GCGC 9 cut(s) 37, 335, 408, 940, 1115, 1117, 1132, 1191, 1196
Cfr10I RCCGGY 2 cut(s) 828, 889
Cfr9I CCCGGG 1 cut(s) 1273
CseI GACGC 4 cut(s) 107, 722, 1036, 1306
Csp6I GTAC 5 cut(s) 24, 496, 604, 778, 931
CviAII CATG 6 cut(s) 330, 343, 490, 613, 679, 906
CviQI GTAC 5 cut(s) 24, 496, 604, 778, 931
DdeI CTNAG 4 cut(s) 557, 582, 885, 1280
DinI GGCGCC 1 cut(s) 939
DpnI GATC 6 cut(s) 413, 664, 998, 1016, 1232, 1326
DpnII GATC 6 cut(s) 411, 662, 996, 1014, 1230, 1324
EaeI YGGCCR 1 cut(s) 1259
EagI CGGCCG 1 cut(s) 1259
Eam1104I CTCTTC 1 cut(s) 881
EarI CTCTTC 1 cut(s) 881
EciI GGCGGA 4 cut(s) 199, 221, 515, 1039
EclXI CGGCCG 1 cut(s) 1259
Eco130I CCWWGG 1 cut(s) 366
Eco147I AGGCCT 2 cut(s) 365, 467
Eco24I GRGCYC 1 cut(s) 390
Eco31I GGTCTC 1 cut(s) 627
Eco32I GATATC 1 cut(s) 1143
Eco47I GGWCC 8 cut(s) 14, 586, 840, 921, 960, 1135, 1162, 1270
Eco52I CGGCCG 1 cut(s) 1259
Eco57I CTGAAG 1 cut(s) 659
Eco88I CYCGRG 3 cut(s) 793, 1178, 1273
EcoO109I RGGNCCY 5 cut(s) 586, 921, 960, 1135, 1162
EcoRII CCWGG 1 cut(s) 588
EcoRV GATATC 1 cut(s) 1143
EcoT14I CCWWGG 1 cut(s) 366
EcoT38I GRGCYC 1 cut(s) 390
EgeI GGCGCC 1 cut(s) 939
EheI GGCGCC 1 cut(s) 939
ErhI CCWWGG 1 cut(s) 366
FaeI CATG 6 cut(s) 333, 346, 493, 616, 682, 909
FaiI YATR 7 cut(s) 331, 344, 491, 531, 614, 680, 907
FalI AAGNNNNNCTT 2 cut(s) 350, 382
FaqI GGGAC 5 cut(s) 279, 934, 946, 1148, 1256
FatI CATG 6 cut(s) 329, 342, 489, 612, 678, 905
FauI CCCGC 3 cut(s) 730, 791, 1125
FbaI TGATCA 1 cut(s) 1324
FblI GTMKAC 2 cut(s) 273, 354
FokI GGATG 2 cut(s) 304, 1200
FriOI GRGCYC 1 cut(s) 390
FspBI CTAG 1 cut(s) 755
GlaI GCGC 9 cut(s) 36, 334, 407, 939, 1114, 1116, 1131, 1190, 1195
HaeII RGCGCY 2 cut(s) 941, 1197
HaeIII GGCC 5 cut(s) 365, 371, 467, 1125, 1261
HgaI GACGC 4 cut(s) 107, 722, 1036, 1306
HhaI GCGC 9 cut(s) 37, 335, 408, 940, 1115, 1117, 1132, 1191, 1196
Hin1I GRCGYC 4 cut(s) 938, 1028, 1203, 1298
Hin1II CATG 6 cut(s) 333, 346, 493, 616, 682, 909
Hin6I GCGC 9 cut(s) 35, 333, 406, 938, 1113, 1115, 1130, 1189, 1194
HinP1I GCGC 9 cut(s) 35, 333, 406, 938, 1113, 1115, 1130, 1189, 1194
HincII GTYRAC 2 cut(s) 355, 979
HindII GTYRAC 2 cut(s) 355, 979
HinfI GANTC 8 cut(s) 41, 145, 299, 305, 445, 731, 740, 758
HphI GGTGA 1 cut(s) 726
Hpy166II GTNNAC 3 cut(s) 274, 355, 979
Hpy188III TCNNGA 9 cut(s) 296, 376, 442, 755, 843, 919, 1072, 1093, 1207
Hpy8I GTNNAC 3 cut(s) 274, 355, 979
Hpy99I CGWCG 6 cut(s) 101, 356, 671, 716, 920, 1300
HpyAV CCTTC 5 cut(s) 103, 376, 820, 1099, 1278
HpyCH4III ACNGT 5 cut(s) 50, 271, 671, 1251, 1347
HpyCH4IV ACGT 3 cut(s) 651, 933, 1203
HpyCH4V TGCA 4 cut(s) 282, 483, 517, 1110
HpyF10VI GCNNNNNNNGC 9 cut(s) 81, 231, 240, 249, 264, 288, 507, 722, 1261
HpyF3I CTNAG 4 cut(s) 557, 582, 885, 1280
HpySE526I ACGT 3 cut(s) 651, 933, 1203
Hsp92I GRCGYC 4 cut(s) 938, 1028, 1203, 1298
Hsp92II CATG 6 cut(s) 333, 346, 493, 616, 682, 909
HspAI GCGC 9 cut(s) 35, 333, 406, 938, 1113, 1115, 1130, 1189, 1194
KasI GGCGCC 1 cut(s) 937
KflI GGGWCCC 1 cut(s) 921
Ksp22I TGATCA 1 cut(s) 1324
Kzo9I GATC 6 cut(s) 411, 662, 996, 1014, 1230, 1324
Lsp1109I GCAGC 5 cut(s) 294, 565, 1122, 1183, 1242
LweI GCATC 4 cut(s) 255, 492, 1077, 1222
MaeI CTAG 1 cut(s) 755
MaeII ACGT 3 cut(s) 651, 933, 1203
MaeIII GTNAC 3 cut(s) 62, 671, 732
MalI GATC 6 cut(s) 413, 664, 998, 1016, 1232, 1326
MbiI CCGCTC 2 cut(s) 168, 1171
MboI GATC 6 cut(s) 411, 662, 996, 1014, 1230, 1324
MboII GAAGA 5 cut(s) 124, 391, 868, 991, 1213
MflI RGATCY 3 cut(s) 411, 662, 996
MhlI GDGCHC 3 cut(s) 390, 657, 800
Mly113I GGCGCC 1 cut(s) 938
MlyI GAGTC 6 cut(s) 293, 299, 439, 734, 740, 767
MmeI TCCRAC 5 cut(s) 198, 327, 690, 711, 735
MseI TTAA 2 cut(s) 113, 1085
MslI CAYNNNNRTG 1 cut(s) 488
MspR9I CCNGG 5 cut(s) 590, 1147, 1166, 1274, 1275
MteI GCGCNGCGC 1 cut(s) 1192
MvaI CCWGG 1 cut(s) 590
MvnI CGCG 8 cut(s) 35, 37, 225, 974, 1115, 1130, 1132, 1191
MwoI GCNNNNNNNGC 9 cut(s) 81, 231, 240, 249, 264, 288, 507, 722, 1261
NarI GGCGCC 1 cut(s) 938
NciI CCSGG 4 cut(s) 1147, 1166, 1274, 1275
NdeII GATC 6 cut(s) 411, 662, 996, 1014, 1230, 1324
NlaIII CATG 6 cut(s) 333, 346, 493, 616, 682, 909
NmeAIII GCCGAG 2 cut(s) 421, 481
NmuCI GTSAC 3 cut(s) 62, 671, 732
NotI GCGGCCGC 1 cut(s) 1259
NspI RCATGY 1 cut(s) 616
PauI GCGCGC 1 cut(s) 1113
PceI AGGCCT 2 cut(s) 365, 467
PciI ACATGT 1 cut(s) 612
PcsI WCGNNNNNNNCGW 5 cut(s) 186, 255, 459, 663, 924
PfeI GAWTC 2 cut(s) 41, 145
Pfl23II CGTACG 1 cut(s) 777
PflFI GACNNNGTC 1 cut(s) 859
PflMI CCANNNNNTGG 1 cut(s) 616
PleI GAGTC 6 cut(s) 293, 299, 439, 734, 739, 766
PluTI GGCGCC 1 cut(s) 941
PpsI GAGTC 6 cut(s) 293, 299, 439, 734, 739, 766
Ppu21I YACGTR 1 cut(s) 934
PpuMI RGGWCCY 5 cut(s) 586, 921, 960, 1135, 1162
PscI ACATGT 1 cut(s) 612
Psp5II RGGWCCY 5 cut(s) 586, 921, 960, 1135, 1162
Psp6I CCWGG 1 cut(s) 588
PspGI CCWGG 1 cut(s) 588
PspLI CGTACG 1 cut(s) 777
PspPPI RGGWCCY 5 cut(s) 586, 921, 960, 1135, 1162
PstI CTGCAG 2 cut(s) 284, 1112
PsuI RGATCY 3 cut(s) 411, 662, 996
PsyI GACNNNGTC 1 cut(s) 859
PteI GCGCGC 1 cut(s) 1113
RsaI GTAC 5 cut(s) 25, 497, 605, 779, 932
RsaNI GTAC 5 cut(s) 24, 496, 604, 778, 931
RseI CAYNNNNRTG 1 cut(s) 488
SalI GTCGAC 1 cut(s) 353
SaqAI TTAA 2 cut(s) 113, 1085
Sau3AI GATC 6 cut(s) 411, 662, 996, 1014, 1230, 1324
ScaI AGTACT 2 cut(s) 25, 497
SchI GAGTC 6 cut(s) 293, 299, 439, 734, 740, 767
ScrFI CCNGG 5 cut(s) 590, 1147, 1166, 1274, 1275
SduI GDGCHC 3 cut(s) 390, 657, 800
SfaNI GCATC 4 cut(s) 255, 492, 1077, 1222
SfcI CTRYAG 4 cut(s) 70, 280, 628, 1108
SfoI GGCGCC 1 cut(s) 939
SinI GGWCC 8 cut(s) 14, 586, 840, 921, 960, 1135, 1162, 1270
SmaI CCCGGG 1 cut(s) 1275
SmiMI CAYNNNNRTG 1 cut(s) 488
SmlI CTYRAG 3 cut(s) 155, 374, 1118
SmoI CTYRAG 3 cut(s) 155, 374, 1118
SseBI AGGCCT 2 cut(s) 365, 467
SspDI GGCGCC 1 cut(s) 937
SspMI CTAG 1 cut(s) 755
StuI AGGCCT 2 cut(s) 365, 467
StyD4I CCNGG 5 cut(s) 588, 1145, 1164, 1272, 1273
StyI CCWWGG 1 cut(s) 366
TaaI ACNGT 5 cut(s) 50, 271, 671, 1251, 1347
TaiI ACGT 3 cut(s) 654, 936, 1206
TaqI TCGA 6 cut(s) 96, 354, 462, 1046, 1184, 1359
TatI WGTACW 2 cut(s) 23, 495
TfiI GAWTC 2 cut(s) 41, 145
Tru1I TTAA 2 cut(s) 113, 1085
Tru9I TTAA 2 cut(s) 113, 1085
TscAI CASTG 1 cut(s) 1065
TseFI GTSAC 3 cut(s) 62, 671, 732
TseI GCWGC 5 cut(s) 282, 578, 1110, 1196, 1255
Tsp45I GTSAC 3 cut(s) 62, 671, 732
TspDTI ATGAA 2 cut(s) 359, 894
TspGWI ACGGA 2 cut(s) 178, 1356
TspMI CCCGGG 1 cut(s) 1273
TspRI CASTG 1 cut(s) 1065
Tth111I GACNNNGTC 1 cut(s) 859
Van91I CCANNNNNTGG 1 cut(s) 616
VpaK11BI GGWCC 8 cut(s) 14, 586, 840, 921, 960, 1135, 1162, 1270
XbaI TCTAGA 1 cut(s) 754
XceI RCATGY 1 cut(s) 616
XcmI CCANNNNNNNNNTGG 1 cut(s) 397
XmaI CCCGGG 1 cut(s) 1273
XmiI GTMKAC 2 cut(s) 273, 354
XspI CTAG 1 cut(s) 755
ZraI GACGTC 1 cut(s) 1204
ZrmI AGTACT 2 cut(s) 25, 497
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.