Rh5BG119100

Protein kinase PINOID-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0012958
Physical Location & Seq
Reverse (-)
11195730 .. 11198557
2828 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG119100.1

Sequence Viewer

Length: 1380 bp
ATGTTAGAGCTTGGTCCGATTAGTACTACTCTCGCGCGTGATTCAGACAGTTCAATGAGTAGTGACACCTGTAGCAGTTTTAGCCGCCTCTCCTTCGACGCAACCGCCACCTTAACCGCCGCAACCGACTTCTTCGGCACCGAGAATCTCGCCCTCAAGCCGCACCGCTCCTCCGACTTCGCCTACTCCGTCATTCGCTCCAAGAAATCCGCCCTCACTTACCGCGACTTCCGCCTCCACCGCCGCATCGGCTCCGGCGACATCGGCACCGTCTACCTCTGCAGCCTCCGCAGTCCCGACTCCGACTCATCATCATCATCATCATCCTCCTCAACCTCATGCGCCTACGCCATGAAAGTCGTCGACAAGGAGGCCTTGGCCCTCAAGAAGAAGGAGCCCAGAGCCGAGATGGAGCGCAAGATCCTCAAAATGCTTGACCACCCTTTCCTCCCGACTCTCTACGCCGAGTTCGAGGCCTCGCATTTCTCCTGCATCGTCATGGAGTACTGCTCCGGCGGCGACTTGCACTCCCTCCGCCATAAACAGCCTAACCGCCGCTTCTCTCTCAGCTCCGCAAGGTTCTACGCTGCTGAGGTCCTGGTGGCTTTGGAGTACCTCCACATGTTGGGAATCATCTACAGAGACCTGAAGCCGGAGAACGTGCTCGTCAGATCCGACGGTCACATCATGCTCTCCGACTTTGACCTCTCTCTCTGCTCCGACGCAATCCCCGCCGTTGAATCACCCGACTCCTCTCTGGTTCTAGAGTCCATCTCAACTCCATCGTACGCCCGCCCCCACCCGAGCACTCTCACTCCCTTCTCCTGCCTCTCAAACCGGCTGTTCCGGTCCAGAAAGGTTCAGACTTTGACGCCGAACCGCCTCTTCGTGGCTGAGCCGGTCGCCGCCCGGTCATGCTCGTTCGTCGGGACCCACGAGTACGTCGCGCCGGAGGTTGCTTCCGGTAGGTCCCACGGCAACGCGGTTGACTGGTGGGCGTTTGGGATCTTCCTCTACGAGATGATCTACGGGCGGACGCCGTTTGCGGCTCCATCGAACGAGACTACACTGCGAAGCATCGTGAAAAGTCCCTTAACCTTCCCGACGTCCACGCCCTGCAGCGCGCTTGAGGGCCACGCGCGGGACCTGATATCCGGGTTTCTGAACAAGGACCCGGAGCGGCGGCTCGGGTCGAAGCGCGGCGCTGCTGACGTCAAGAAGCATCCTTTCTTCAAGGGGATCAACTTGGCGTTGATACGGTCGCTGCGGCCGCCGATGGTCCCGGGTCTGAGAAAGCAGAACACGACGCCGTATTACCACGCCAAGTCTGATGATCAGAACAAAAATAACGGACAGTCTGGGGCGTTCGATTACTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000902 GO:0000904 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008152 GO:0009605 GO:0009606 GO:0009628 GO:0009629 GO:0009630 GO:0009653 GO:0009719 GO:0009725 GO:0009733 GO:0009734 GO:0009755 GO:0009790 GO:0009791 GO:0009793 GO:0009888 GO:0009908 GO:0009914 GO:0009926 GO:0009958 GO:0009986 GO:0009987 GO:0010015 GO:0010033 GO:0010053 GO:0010054 GO:0010154 GO:0010817 GO:0016020 GO:0016043 GO:0016049 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0021700 GO:0022414 GO:0022622 GO:0023052 GO:0030154 GO:0032501 GO:0032502 GO:0032870 GO:0032879 GO:0032989 GO:0035556 GO:0036211 GO:0040007 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0048316 GO:0048364 GO:0048366 GO:0048367 GO:0048468 GO:0048469 GO:0048588 GO:0048589 GO:0048608 GO:0048731 GO:0048764 GO:0048765 GO:0048766 GO:0048767 GO:0048825 GO:0048827 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051049 GO:0051179 GO:0051234 GO:0051716 GO:0060560 GO:0060918 GO:0061458 GO:0065007 GO:0065008 GO:0070887 GO:0071310 GO:0071365 GO:0071495 GO:0071695 GO:0071704 GO:0071840 GO:0071944 GO:0080147 GO:0080167 GO:0090558 GO:0090567 GO:0090627 GO:0099402 GO:0140096 GO:1901564 GO:1905392 GO:2000012
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

459

Amino Acids

50.84

Weight (kDa)

8.92

Isoelectric Point (pI)

52.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 78 - 237 5.9e-31 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 78 - 237 1e-19 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 305 - 411 2.5e-19 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 313 - 395 5.1e-06 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012958)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G34650
fragaria_vesca FvH4_3g09660
malus_domestica MD00G1109900.v1.1 MD05G1276300.v1.1
prunus_persica Prupe.4G088000_v2.0.a1 Prupe.4G088000_v2.0.a1
pyrus_communis pycom05g25730 pycom10g21130
rosa_chinensis RchiOBHm_Chr5g0015331
rosa_laevigata RLG00000032193
rosa_multiflora Rmu_co8298181.1_g000001
rosa_roxburghii Rroxscaffold_1G00061120
rosa_rugosa Rorug05G0026700
rosa_samantha Rh5AG120200 Rh5BG119100 Rh5CG129600 Rh5DG118000
rosa_wichuraiana Rw5G010390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 1109, 1215
AccB1I GGYRCC 2 cut(s) 137, 266
AccB7I CCANNNNNTGG 1 cut(s) 625
AccBSI CCGCTC 2 cut(s) 168, 1180
AccI GTMKAC 2 cut(s) 273, 363
AccII CGCG 9 cut(s) 35, 37, 225, 947, 983, 1124, 1139, 1141, 1200
AclWI GGATC 4 cut(s) 415, 666, 1013, 1247
AcoI YGGCCR 1 cut(s) 1268
AcuI CTGAAG 1 cut(s) 668
AcyI GRCGYC 5 cut(s) 872, 1037, 1106, 1212, 1307
AfaI GTAC 5 cut(s) 25, 506, 614, 788, 941
AfiI CCNNNNNNNGG 4 cut(s) 625, 652, 889, 1141
AflIII ACRYGT 1 cut(s) 621
AgsI TTSAA 3 cut(s) 54, 740, 1234
AjnI CCWGG 1 cut(s) 597
AluBI AGCT 2 cut(s) 10, 570
AluI AGCT 2 cut(s) 10, 570
Alw21I GWGCWC 2 cut(s) 666, 809
Alw26I GTCTC 2 cut(s) 636, 1055
AlwI GGATC 4 cut(s) 415, 666, 1013, 1247
Ama87I CYCGRG 3 cut(s) 802, 1187, 1282
AoxI GGCC 5 cut(s) 372, 378, 474, 1132, 1268
ApeKI GCWGC 5 cut(s) 282, 587, 1119, 1205, 1264
AspLEI GCGC 9 cut(s) 37, 344, 417, 949, 1124, 1126, 1141, 1200, 1205
AsuC2I CCSGG 5 cut(s) 910, 1156, 1175, 1283, 1284
AsuHPI GGTGA 1 cut(s) 735
AvaI CYCGRG 3 cut(s) 802, 1187, 1282
AvaII GGWCC 8 cut(s) 14, 595, 849, 930, 969, 1144, 1171, 1279
BanI GGYRCC 2 cut(s) 137, 266
BanII GRGCYC 1 cut(s) 399
BauI CACGAG 1 cut(s) 935
Bbv12I GWGCWC 2 cut(s) 666, 809
BbvCI CCTCAGC 1 cut(s) 591
BbvI GCAGC 5 cut(s) 294, 574, 1131, 1192, 1251
BccI CCATC 5 cut(s) 403, 779, 790, 1060, 1270
BceAI ACGGC 4 cut(s) 719, 991, 1024, 1294
BciT130I CCWGG 1 cut(s) 599
BclI TGATCA 1 cut(s) 1333
BcnI CCSGG 5 cut(s) 910, 1156, 1175, 1283, 1284
BcoDI GTCTC 2 cut(s) 636, 1055
BfaI CTAG 1 cut(s) 764
BfmI CTRYAG 4 cut(s) 70, 280, 637, 1117
BfoI RGCGCY 1 cut(s) 1206
BglI GCCNNNNNGGC 1 cut(s) 249
BlpI GCTNAGC 1 cut(s) 894
BmcAI AGTACT 2 cut(s) 25, 506
Bme1390I CCNGG 6 cut(s) 599, 910, 1156, 1175, 1283, 1284
Bme18I GGWCC 8 cut(s) 14, 595, 849, 930, 969, 1144, 1171, 1279
BmeT110I CYCGRG 3 cut(s) 802, 1187, 1282
BmrFI CCNGG 6 cut(s) 599, 910, 1156, 1175, 1283, 1284
BmsI GCATC 4 cut(s) 255, 501, 1086, 1231
BplI GAGNNNNNCTC 4 cut(s) 494, 526, 758, 790
Bpu10I CCTNAGC 1 cut(s) 591
Bpu1102I GCTNAGC 1 cut(s) 894
BpuEI CTTGAG 3 cut(s) 140, 368, 1148
BpuMI CCSGG 5 cut(s) 910, 1156, 1175, 1283, 1284
BsaHI GRCGYC 5 cut(s) 872, 1037, 1106, 1212, 1307
BsaI GGTCTC 1 cut(s) 636
BsaJI CCNNGG 3 cut(s) 375, 973, 1282
BsaWI WCCGGW 2 cut(s) 846, 962
BsaXI ACNNNNNCTCC 6 cut(s) 155, 185, 512, 542, 799, 829
Bsc4I CCNNNNNNNGG 4 cut(s) 625, 652, 889, 1141
Bse118I RCCGGY 2 cut(s) 837, 898
Bse1I ACTGG 1 cut(s) 995
BseBI CCWGG 1 cut(s) 599
BseDI CCNNGG 3 cut(s) 375, 973, 1282
BseGI GGATG 2 cut(s) 323, 1222
BseLI CCNNNNNNNGG 4 cut(s) 625, 652, 889, 1141
BseMII CTCAG 4 cut(s) 580, 582, 885, 1280
BseNI ACTGG 1 cut(s) 995
BsePI GCGCGC 1 cut(s) 1122
BseRI GAGGAG 3 cut(s) 160, 319, 742
BseX3I CGGCCG 1 cut(s) 1268
BseXI GCAGC 5 cut(s) 294, 574, 1131, 1192, 1251
Bsh1236I CGCG 9 cut(s) 35, 37, 225, 947, 983, 1124, 1139, 1141, 1200
Bsh1285I CGRYCG 3 cut(s) 903, 1262, 1271
BshFI GGCC 5 cut(s) 374, 380, 476, 1134, 1270
BshNI GGYRCC 2 cut(s) 137, 266
BsiEI CGRYCG 3 cut(s) 903, 1262, 1271
BsiHKAI GWGCWC 2 cut(s) 666, 809
BsiHKCI CYCGRG 3 cut(s) 802, 1187, 1282
BsiWI CGTACG 1 cut(s) 786
BslFI GGGAC 6 cut(s) 279, 943, 955, 1074, 1157, 1265
BslI CCNNNNNNNGG 4 cut(s) 625, 652, 889, 1141
BsmAI GTCTC 2 cut(s) 636, 1055
BsmFI GGGAC 6 cut(s) 279, 943, 955, 1074, 1157, 1265
BsnI GGCC 5 cut(s) 374, 380, 476, 1134, 1270
Bso31I GGTCTC 1 cut(s) 636
BsoBI CYCGRG 3 cut(s) 802, 1187, 1282
Bsp1286I GDGCHC 3 cut(s) 399, 666, 809
Bsp143I GATC 6 cut(s) 420, 671, 1005, 1023, 1239, 1333
Bsp1720I GCTNAGC 1 cut(s) 894
BspANI GGCC 5 cut(s) 374, 380, 476, 1134, 1270
BspCNI CTCAG 4 cut(s) 579, 583, 886, 1281
BspFNI CGCG 9 cut(s) 35, 37, 225, 947, 983, 1124, 1139, 1141, 1200
BspMAI CTGCAG 2 cut(s) 284, 1121
BspPI GGATC 4 cut(s) 415, 666, 1013, 1247
BspT107I GGYRCC 2 cut(s) 137, 266
BspTNI GGTCTC 1 cut(s) 636
BsrBI CCGCTC 2 cut(s) 168, 1180
BsrFI RCCGGY 2 cut(s) 837, 898
BsrI ACTGG 1 cut(s) 995
BssAI RCCGGY 2 cut(s) 837, 898
BssECI CCNNGG 3 cut(s) 375, 973, 1282
BssHII GCGCGC 1 cut(s) 1122
BssMI GATC 6 cut(s) 420, 671, 1005, 1023, 1239, 1333
BssNI GRCGYC 5 cut(s) 872, 1037, 1106, 1212, 1307
BssSI CACGAG 1 cut(s) 935
BssT1I CCWWGG 1 cut(s) 375
Bst2BI CACGAG 1 cut(s) 935
Bst2UI CCWGG 1 cut(s) 599
Bst4CI ACNGT 5 cut(s) 50, 271, 680, 1260, 1356
Bst6I CTCTTC 1 cut(s) 890
BstACI GRCGYC 5 cut(s) 872, 1037, 1106, 1212, 1307
BstC8I GCNNGC 2 cut(s) 793, 1124
BstDEI CTNAG 4 cut(s) 566, 591, 894, 1289
BstDSI CCRYGG 1 cut(s) 973
BstF5I GGATG 2 cut(s) 323, 1222
BstFNI CGCG 9 cut(s) 35, 37, 225, 947, 983, 1124, 1139, 1141, 1200
BstH2I RGCGCY 1 cut(s) 1206
BstHHI GCGC 9 cut(s) 37, 344, 417, 949, 1124, 1126, 1141, 1200, 1205
BstKTI GATC 6 cut(s) 423, 674, 1008, 1026, 1242, 1336
BstMAI GTCTC 2 cut(s) 636, 1055
BstMBI GATC 6 cut(s) 420, 671, 1005, 1023, 1239, 1333
BstMCI CGRYCG 3 cut(s) 903, 1262, 1271
BstMWI GCNNNNNNNGC 9 cut(s) 81, 231, 240, 249, 264, 288, 516, 731, 1270
BstNI CCWGG 1 cut(s) 599
BstNSI RCATGY 1 cut(s) 625
BstSCI CCNGG 6 cut(s) 597, 908, 1154, 1173, 1281, 1282
BstSFI CTRYAG 4 cut(s) 70, 280, 637, 1117
BstUI CGCG 9 cut(s) 35, 37, 225, 947, 983, 1124, 1139, 1141, 1200
BstV1I GCAGC 5 cut(s) 294, 574, 1131, 1192, 1251
BstX2I RGATCY 3 cut(s) 420, 671, 1005
BstYI RGATCY 3 cut(s) 420, 671, 1005
BstZI CGGCCG 1 cut(s) 1268
BsuRI GGCC 5 cut(s) 374, 380, 476, 1134, 1270
BtgI CCRYGG 1 cut(s) 973
BtsCI GGATG 2 cut(s) 323, 1222
BtsI GCAGTG 1 cut(s) 1067
BtsIMutI CAGTG 1 cut(s) 1067
Cac8I GCNNGC 2 cut(s) 793, 1124
CciNI GCGGCCGC 1 cut(s) 1268
CfoI GCGC 9 cut(s) 37, 344, 417, 949, 1124, 1126, 1141, 1200, 1205
Cfr10I RCCGGY 2 cut(s) 837, 898
Cfr9I CCCGGG 1 cut(s) 1282
CseI GACGC 5 cut(s) 107, 731, 880, 1045, 1315
Csp6I GTAC 5 cut(s) 24, 505, 613, 787, 940
CviAII CATG 6 cut(s) 339, 352, 499, 622, 688, 915
CviQI GTAC 5 cut(s) 24, 505, 613, 787, 940
DdeI CTNAG 4 cut(s) 566, 591, 894, 1289
DpnI GATC 6 cut(s) 422, 673, 1007, 1025, 1241, 1335
DpnII GATC 6 cut(s) 420, 671, 1005, 1023, 1239, 1333
EaeI YGGCCR 1 cut(s) 1268
EagI CGGCCG 1 cut(s) 1268
Eam1104I CTCTTC 1 cut(s) 890
EarI CTCTTC 1 cut(s) 890
EciI GGCGGA 4 cut(s) 199, 221, 524, 1048
EclXI CGGCCG 1 cut(s) 1268
Eco130I CCWWGG 1 cut(s) 375
Eco147I AGGCCT 2 cut(s) 374, 476
Eco24I GRGCYC 1 cut(s) 399
Eco31I GGTCTC 1 cut(s) 636
Eco32I GATATC 1 cut(s) 1152
Eco47I GGWCC 8 cut(s) 14, 595, 849, 930, 969, 1144, 1171, 1279
Eco52I CGGCCG 1 cut(s) 1268
Eco57I CTGAAG 1 cut(s) 668
Eco88I CYCGRG 3 cut(s) 802, 1187, 1282
EcoO109I RGGNCCY 5 cut(s) 595, 930, 969, 1144, 1171
EcoRII CCWGG 1 cut(s) 597
EcoRV GATATC 1 cut(s) 1152
EcoT14I CCWWGG 1 cut(s) 375
EcoT38I GRGCYC 1 cut(s) 399
ErhI CCWWGG 1 cut(s) 375
FaeI CATG 6 cut(s) 342, 355, 502, 625, 691, 918
FaiI YATR 7 cut(s) 340, 353, 500, 540, 623, 689, 916
FalI AAGNNNNNCTT 2 cut(s) 359, 391
FaqI GGGAC 6 cut(s) 279, 943, 955, 1074, 1157, 1265
FatI CATG 6 cut(s) 338, 351, 498, 621, 687, 914
FauI CCCGC 3 cut(s) 739, 800, 1134
FbaI TGATCA 1 cut(s) 1333
FblI GTMKAC 2 cut(s) 273, 363
FokI GGATG 2 cut(s) 310, 1209
FriOI GRGCYC 1 cut(s) 399
FspBI CTAG 1 cut(s) 764
GlaI GCGC 9 cut(s) 36, 343, 416, 948, 1123, 1125, 1140, 1199, 1204
HaeII RGCGCY 1 cut(s) 1206
HaeIII GGCC 5 cut(s) 374, 380, 476, 1134, 1270
HgaI GACGC 5 cut(s) 107, 731, 880, 1045, 1315
HhaI GCGC 9 cut(s) 37, 344, 417, 949, 1124, 1126, 1141, 1200, 1205
Hin1I GRCGYC 5 cut(s) 872, 1037, 1106, 1212, 1307
Hin1II CATG 6 cut(s) 342, 355, 502, 625, 691, 918
Hin6I GCGC 9 cut(s) 35, 342, 415, 947, 1122, 1124, 1139, 1198, 1203
HinP1I GCGC 9 cut(s) 35, 342, 415, 947, 1122, 1124, 1139, 1198, 1203
HincII GTYRAC 2 cut(s) 364, 988
HindII GTYRAC 2 cut(s) 364, 988
HinfI GANTC 9 cut(s) 41, 145, 299, 305, 454, 630, 740, 749, 767
HphI GGTGA 1 cut(s) 735
Hpy166II GTNNAC 4 cut(s) 274, 364, 988, 1110
Hpy188III TCNNGA 9 cut(s) 296, 385, 451, 764, 852, 928, 1081, 1102, 1216
Hpy8I GTNNAC 4 cut(s) 274, 364, 988, 1110
Hpy99I CGWCG 8 cut(s) 101, 365, 680, 725, 929, 947, 1108, 1309
HpyAV CCTTC 4 cut(s) 103, 385, 829, 1108
HpyCH4III ACNGT 5 cut(s) 50, 271, 680, 1260, 1356
HpyCH4IV ACGT 4 cut(s) 660, 942, 1106, 1212
HpyCH4V TGCA 4 cut(s) 282, 492, 526, 1119
HpyF10VI GCNNNNNNNGC 9 cut(s) 81, 231, 240, 249, 264, 288, 516, 731, 1270
HpyF3I CTNAG 4 cut(s) 566, 591, 894, 1289
HpySE526I ACGT 4 cut(s) 660, 942, 1106, 1212
Hsp92I GRCGYC 5 cut(s) 872, 1037, 1106, 1212, 1307
Hsp92II CATG 6 cut(s) 342, 355, 502, 625, 691, 918
HspAI GCGC 9 cut(s) 35, 342, 415, 947, 1122, 1124, 1139, 1198, 1203
KflI GGGWCCC 1 cut(s) 930
Ksp22I TGATCA 1 cut(s) 1333
Kzo9I GATC 6 cut(s) 420, 671, 1005, 1023, 1239, 1333
Lsp1109I GCAGC 5 cut(s) 294, 574, 1131, 1192, 1251
LweI GCATC 4 cut(s) 255, 501, 1086, 1231
MaeI CTAG 1 cut(s) 764
MaeII ACGT 4 cut(s) 660, 942, 1106, 1212
MaeIII GTNAC 2 cut(s) 62, 680
MalI GATC 6 cut(s) 422, 673, 1007, 1025, 1241, 1335
MbiI CCGCTC 2 cut(s) 168, 1180
MboI GATC 6 cut(s) 420, 671, 1005, 1023, 1239, 1333
MboII GAAGA 5 cut(s) 124, 400, 877, 1000, 1222
MflI RGATCY 3 cut(s) 420, 671, 1005
MhlI GDGCHC 3 cut(s) 399, 666, 809
MlyI GAGTC 5 cut(s) 293, 299, 448, 743, 776
MmeI TCCRAC 5 cut(s) 198, 327, 699, 720, 744
MseI TTAA 2 cut(s) 113, 1094
MslI CAYNNNNRTG 1 cut(s) 497
MspR9I CCNGG 6 cut(s) 599, 910, 1156, 1175, 1283, 1284
MteI GCGCNGCGC 1 cut(s) 1201
MvaI CCWGG 1 cut(s) 599
MvnI CGCG 9 cut(s) 35, 37, 225, 947, 983, 1124, 1139, 1141, 1200
MwoI GCNNNNNNNGC 9 cut(s) 81, 231, 240, 249, 264, 288, 516, 731, 1270
NciI CCSGG 5 cut(s) 910, 1156, 1175, 1283, 1284
NdeII GATC 6 cut(s) 420, 671, 1005, 1023, 1239, 1333
NlaIII CATG 6 cut(s) 342, 355, 502, 625, 691, 918
NmeAIII GCCGAG 2 cut(s) 430, 490
NmuCI GTSAC 2 cut(s) 62, 680
NotI GCGGCCGC 1 cut(s) 1268
NspI RCATGY 1 cut(s) 625
PauI GCGCGC 1 cut(s) 1122
PceI AGGCCT 2 cut(s) 374, 476
PciI ACATGT 1 cut(s) 621
PcsI WCGNNNNNNNCGW 5 cut(s) 186, 255, 468, 672, 933
PfeI GAWTC 4 cut(s) 41, 145, 630, 740
Pfl23II CGTACG 1 cut(s) 786
PflMI CCANNNNNTGG 1 cut(s) 625
PleI GAGTC 5 cut(s) 293, 299, 448, 743, 775
PpsI GAGTC 5 cut(s) 293, 299, 448, 743, 775
PpuMI RGGWCCY 5 cut(s) 595, 930, 969, 1144, 1171
PscI ACATGT 1 cut(s) 621
Psp5II RGGWCCY 5 cut(s) 595, 930, 969, 1144, 1171
Psp6I CCWGG 1 cut(s) 597
PspGI CCWGG 1 cut(s) 597
PspLI CGTACG 1 cut(s) 786
PspPPI RGGWCCY 5 cut(s) 595, 930, 969, 1144, 1171
PstI CTGCAG 2 cut(s) 284, 1121
PsuI RGATCY 3 cut(s) 420, 671, 1005
PteI GCGCGC 1 cut(s) 1122
RsaI GTAC 5 cut(s) 25, 506, 614, 788, 941
RsaNI GTAC 5 cut(s) 24, 505, 613, 787, 940
RseI CAYNNNNRTG 1 cut(s) 497
SalI GTCGAC 1 cut(s) 362
SaqAI TTAA 2 cut(s) 113, 1094
Sau3AI GATC 6 cut(s) 420, 671, 1005, 1023, 1239, 1333
ScaI AGTACT 2 cut(s) 25, 506
SchI GAGTC 5 cut(s) 293, 299, 448, 743, 776
ScrFI CCNGG 6 cut(s) 599, 910, 1156, 1175, 1283, 1284
SduI GDGCHC 3 cut(s) 399, 666, 809
SfaNI GCATC 4 cut(s) 255, 501, 1086, 1231
SfcI CTRYAG 4 cut(s) 70, 280, 637, 1117
SinI GGWCC 8 cut(s) 14, 595, 849, 930, 969, 1144, 1171, 1279
SmaI CCCGGG 1 cut(s) 1284
SmiMI CAYNNNNRTG 1 cut(s) 497
SmlI CTYRAG 3 cut(s) 155, 383, 1127
SmoI CTYRAG 3 cut(s) 155, 383, 1127
SseBI AGGCCT 2 cut(s) 374, 476
SspMI CTAG 1 cut(s) 764
StuI AGGCCT 2 cut(s) 374, 476
StyD4I CCNGG 6 cut(s) 597, 908, 1154, 1173, 1281, 1282
StyI CCWWGG 1 cut(s) 375
TaaI ACNGT 5 cut(s) 50, 271, 680, 1260, 1356
TaiI ACGT 4 cut(s) 663, 945, 1109, 1215
TaqI TCGA 6 cut(s) 96, 363, 471, 1055, 1193, 1368
TatI WGTACW 2 cut(s) 23, 504
TfiI GAWTC 4 cut(s) 41, 145, 630, 740
Tru1I TTAA 2 cut(s) 113, 1094
Tru9I TTAA 2 cut(s) 113, 1094
TscAI CASTG 1 cut(s) 1074
TseFI GTSAC 2 cut(s) 62, 680
TseI GCWGC 5 cut(s) 282, 587, 1119, 1205, 1264
Tsp45I GTSAC 2 cut(s) 62, 680
TspDTI ATGAA 1 cut(s) 368
TspGWI ACGGA 2 cut(s) 178, 1365
TspMI CCCGGG 1 cut(s) 1282
TspRI CASTG 1 cut(s) 1074
Van91I CCANNNNNTGG 1 cut(s) 625
VpaK11BI GGWCC 8 cut(s) 14, 595, 849, 930, 969, 1144, 1171, 1279
XbaI TCTAGA 1 cut(s) 763
XceI RCATGY 1 cut(s) 625
XcmI CCANNNNNNNNNTGG 1 cut(s) 406
XmaI CCCGGG 1 cut(s) 1282
XmiI GTMKAC 2 cut(s) 273, 363
XspI CTAG 1 cut(s) 764
ZraI GACGTC 2 cut(s) 1107, 1213
ZrmI AGTACT 2 cut(s) 25, 506
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.