Prupe.4G119100_v2.0.a1

Ribulose-1,5 bisphosphate carboxylase oxygenase large subunit N-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0011165
Physical Location & Seq
Forward (+)
6451028 .. 6455646
4619 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G119100.2

Sequence Viewer

Length: 1470 bp
ATGGCCTCTTCTTCTCTTCCTCTGCATCACCCCTCCCATTGCCTCATCTCCAATCGTCAGTTGAAAGGTTCCAACTATGGATGGTCACCACTACCCAGATATACTGTCCCTACTAAAGACGCAGTAAAAGGCATTCGAATTCAACCCATTAAAGCAAAAGCAGCCACCGAAGCTCCTCCGTTCTCTTTGTTTCAGCCTCCTGAAGCTGAAGGGTCTGCCTCTGAGTTGGAGCCAGCGGACCCTGATTTCTACAAGATAGGGTATGTTAGAAGTATGAGAGCTTATGGAATTGAATTTAAAGAAGGACCAGATGGGTTTGGAGTTTATGCTTCCAAGGATGTTGAACCACTTCGCCGGGCTAGAGTAATAATGGAAATTCCACTAGAACTGATGTTAACTGTAAGCCAAAAGCTCCCATGGATGTTTTTTCCAGATATAGTACCAGTGGGTCATCCAATATTTGATATCATTAACTCAACTGATCCAGAGACAGATTGGGATCTGAGGTTAGCATGCCTTCTTTTGTACGCATTTGATCGGGAAGATAACTTTTGGCAGTTGTATGGTGATTTTTTACCCAGTGCAGATGAGTGCCCCAGCTTGCTTCTAGCTAGTGAGGAGGAGCTTTCAGAACTGCAGAATCCCAATCTTGCTTCAACTATGAGAGAACAGCAACGTCGAGCCTTGAAATTTTGGGAAAAAAATTGGCACTCTGGTGTACCACTAAAAATTAAACGCCTTGCTCGTGATCCTGAGAGATTCATTTGGGCATTGAGTATGGCACAATCACGATGCATTGGCATGCAAATGAGAATTGGTGCACTTGTTCAAGATGCAAACATGCTAATTCCATATGCAGATATGCTAAACCATTCCTTTGAGCCAAATTGTTTTTTCCATTGGCGTTTTAAGGATCGCATGCTGGAAGTGATGATAAATGCTGGAAAACGGATTAAGAAAGGTGACGAGATGACTGTCAATTACATGAGTGGACAACAGAATGACCTGTTGATGCAAAGATATGGTTTCTCTTCACCTGTGAATCCTTGGGATGTGATTCAGTTCTCCGGAAATGCACGTATTCATTTGGATTCCTTCTTGTCAGTATTCAATATATCTGGACTTCCTGAAGAGTACTATCATAACACAGGTCGCCTATCTAATGATGGAGATACTTTTGTTGATGGAGCAATCATAGCAGCGGCAAGAACATTGTCCACTTGGTCAGATGGGGATGTGCCTCCAATCCCAAGCATGGAAAGAAGATCTGTAAAGGAGTTACAGGAAGAATGCCGACAGATGCTGGCAGTGTATCCTACTAACTCTAAGCAAGACCAAAAAATTCTAGATTCGATGTCAGATGCTAGTAGGACACTTGAAGCTGCAATCAAGTATAGGTTGCACAGGAAGTTGTTCGTAGAGAAGGTCATGCAAGCGCTGGATATATACCAAGAGCGGATGTTATTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rubis-subs-bind SET

Protein Analysis

490

Amino Acids

56.02

Weight (kDa)

5.79

Isoelectric Point (pI)

47.72

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011165)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G20130 AT4G20130 AT4G20130
fragaria_vesca FvH4_3g13130 FvH4_3g13130 FvH4_3g13130 FvH4_3g13130 FvH4_3g13130
malus_domestica MD10G1226200.v1.1
prunus_persica Prupe.4G119100_v2.0.a1 Prupe.4G119100_v2.0.a1
pyrus_communis pycom10g19010
rosa_chinensis RchiOBHm_Chr5g0021381
rosa_laevigata RLG00000032608
rosa_multiflora Rmu_sc0004847.1_g000020
rosa_roxburghii Rroxscaffold_1G00057160
rosa_rugosa Rorug05G0066700 Rorug05G0066800
rosa_samantha Rh5CG169100
rosa_wichuraiana Rw5G013880

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 1456
AccIII TCCGGA 1 cut(s) 1067
AciI CCGC 3 cut(s) 236, 1202, 1456
AclWI GGATC 4 cut(s) 476, 507, 743, 921
AcsI RAATTY 5 cut(s) 138, 293, 375, 689, 1341
AcuI CTGAAG 3 cut(s) 222, 228, 1149
AfaI GTAC 4 cut(s) 441, 527, 720, 1136
AfeI AGCGCT 1 cut(s) 1437
AfiI CCNNNNNNNGG 1 cut(s) 1255
AgsI TTSAA 9 cut(s) 64, 143, 293, 344, 657, 688, 830, 1111, 1379
AleI CACNNNNGTG 1 cut(s) 714
AluBI AGCT 8 cut(s) 173, 206, 281, 412, 600, 611, 625, 1382
AluI AGCT 8 cut(s) 173, 206, 281, 412, 600, 611, 625, 1382
Alw21I GWGCWC 1 cut(s) 823
Alw26I GTCTC 1 cut(s) 482
Alw44I GTGCAC 1 cut(s) 819
AlwI GGATC 4 cut(s) 476, 507, 743, 921
AlwNI CAGNNNCTG 1 cut(s) 1303
Aor13HI TCCGGA 1 cut(s) 1067
Aor51HI AGCGCT 1 cut(s) 1437
AoxI GGCC 1 cut(s) 3
ApaLI GTGCAC 1 cut(s) 819
ApeKI GCWGC 3 cut(s) 161, 1199, 1382
ApoI RAATTY 5 cut(s) 138, 293, 375, 689, 1341
Asp700I GAANNNNTTC 2 cut(s) 348, 1412
AspLEI GCGC 1 cut(s) 1438
AspS9I GGNCC 2 cut(s) 238, 305
AsuC2I CCSGG 1 cut(s) 356
AsuHPI GGTGA 5 cut(s) 20, 78, 578, 974, 1026
AsuII TTCGAA 1 cut(s) 136
AvaII GGWCC 2 cut(s) 238, 305
BaeGI GKGCMC 2 cut(s) 596, 823
BaeI ACNNNNGTAYC 2 cut(s) 1164, 1197
BauI CACGAG 1 cut(s) 744
Bbv12I GWGCWC 1 cut(s) 823
BbvI GCAGC 3 cut(s) 173, 1211, 1369
BccI CCATC 5 cut(s) 75, 305, 1160, 1178, 1223
BciVI GTATCC 1 cut(s) 1323
BcnI CCSGG 1 cut(s) 356
BcoDI GTCTC 1 cut(s) 482
BfaI CTAG 6 cut(s) 360, 383, 608, 612, 1346, 1365
BfmI CTRYAG 1 cut(s) 635
BfoI RGCGCY 1 cut(s) 1439
BfuI GTATCC 1 cut(s) 1323
BglII AGATCT 1 cut(s) 1265
BisI GCNGC 4 cut(s) 162, 1200, 1203, 1383
BlsI GCNGC 4 cut(s) 163, 1201, 1204, 1384
BmcAI AGTACT 1 cut(s) 1136
Bme1390I CCNGG 1 cut(s) 356
Bme18I GGWCC 2 cut(s) 238, 305
BmgT120I GGNCC 2 cut(s) 238, 305
BmiI GGNNCC 3 cut(s) 70, 231, 240
BmrFI CCNGG 1 cut(s) 356
BmrI ACTGGG 1 cut(s) 573
BmsI GCATC 6 cut(s) 34, 782, 823, 1002, 1290, 1351
BmuI ACTGGG 1 cut(s) 573
Bpu14I TTCGAA 1 cut(s) 136
BpuMI CCSGG 1 cut(s) 356
BsaAI YACGTR 1 cut(s) 1079
BsaBI GATNNNNATC 1 cut(s) 498
BsaJI CCNNGG 3 cut(s) 333, 416, 1046
BsaWI WCCGGW 1 cut(s) 1067
BsaXI ACNNNNNCTCC 2 cut(s) 157, 187
Bsc4I CCNNNNNNNGG 1 cut(s) 1255
Bse1I ACTGG 2 cut(s) 443, 579
Bse3DI GCAATG 1 cut(s) 37
Bse8I GATNNNNATC 1 cut(s) 498
BseAI TCCGGA 1 cut(s) 1067
BseDI CCNNGG 3 cut(s) 333, 416, 1046
BseGI GGATG 7 cut(s) 86, 343, 426, 451, 1057, 1240, 1464
BseJI GATNNNNATC 1 cut(s) 498
BseLI CCNNNNNNNGG 1 cut(s) 1255
BseMI GCAATG 1 cut(s) 37
BseMII CTCAG 3 cut(s) 213, 494, 744
BseNI ACTGG 2 cut(s) 443, 579
BseRI GAGGAG 3 cut(s) 165, 632, 635
BseSI GKGCMC 2 cut(s) 596, 823
BseXI GCAGC 3 cut(s) 173, 1211, 1369
BseYI CCCAGC 1 cut(s) 596
BsgI GTGCAG 1 cut(s) 603
BshFI GGCC 1 cut(s) 5
BsiHKAI GWGCWC 1 cut(s) 823
BsiSI CCGG 2 cut(s) 355, 1068
BslFI GGGAC 1 cut(s) 92
BslI CCNNNNNNNGG 1 cut(s) 1255
BsmAI GTCTC 1 cut(s) 482
BsmFI GGGAC 1 cut(s) 92
BsmI GAATGC 2 cut(s) 132, 1295
BsnI GGCC 1 cut(s) 5
Bsp119I TTCGAA 1 cut(s) 136
Bsp1286I GDGCHC 2 cut(s) 596, 823
Bsp13I TCCGGA 1 cut(s) 1067
Bsp143I GATC 6 cut(s) 481, 499, 535, 748, 913, 1265
Bsp19I CCATGG 1 cut(s) 416
BspACI CCGC 3 cut(s) 236, 1202, 1456
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 3 cut(s) 214, 495, 745
BspEI TCCGGA 1 cut(s) 1067
BspLI GGNNCC 3 cut(s) 70, 231, 240
BspMAI CTGCAG 1 cut(s) 639
BspPI GGATC 4 cut(s) 476, 507, 743, 921
BspT104I TTCGAA 1 cut(s) 136
BsrBI CCGCTC 1 cut(s) 1456
BsrDI GCAATG 1 cut(s) 37
BsrI ACTGG 2 cut(s) 443, 579
BssECI CCNNGG 3 cut(s) 333, 416, 1046
BssMI GATC 6 cut(s) 481, 499, 535, 748, 913, 1265
BssSI CACGAG 1 cut(s) 744
BssT1I CCWWGG 3 cut(s) 333, 416, 1046
Bst2BI CACGAG 1 cut(s) 744
Bst4CI ACNGT 3 cut(s) 106, 400, 976
Bst6I CTCTTC 4 cut(s) 13, 21, 1036, 1125
BstBAI YACGTR 1 cut(s) 1079
BstBI TTCGAA 1 cut(s) 136
BstC8I GCNNGC 7 cut(s) 234, 514, 602, 803, 920, 1305, 1434
BstDEI CTNAG 4 cut(s) 222, 503, 753, 1326
BstDSI CCRYGG 1 cut(s) 416
BstEII GGTNACC 1 cut(s) 84
BstF5I GGATG 7 cut(s) 86, 343, 426, 451, 1057, 1240, 1464
BstH2I RGCGCY 1 cut(s) 1439
BstHHI GCGC 1 cut(s) 1438
BstKTI GATC 6 cut(s) 484, 502, 538, 751, 916, 1268
BstMAI GTCTC 1 cut(s) 482
BstMBI GATC 6 cut(s) 481, 499, 535, 748, 913, 1265
BstMWI GCNNNNNNNGC 3 cut(s) 161, 170, 1196
BstNSI RCATGY 4 cut(s) 516, 805, 844, 922
BstPI GGTNACC 1 cut(s) 84
BstSCI CCNGG 1 cut(s) 354
BstSFI CTRYAG 1 cut(s) 635
BstSLI GKGCMC 2 cut(s) 596, 823
BstV1I GCAGC 3 cut(s) 173, 1211, 1369
BstX2I RGATCY 2 cut(s) 499, 1265
BstYI RGATCY 2 cut(s) 499, 1265
BsuI GTATCC 1 cut(s) 1323
BsuRI GGCC 1 cut(s) 5
BtgI CCRYGG 1 cut(s) 416
BtsCI GGATG 7 cut(s) 86, 343, 426, 451, 1057, 1240, 1464
BtsI GCAGTG 1 cut(s) 1314
BtsIMutI CAGTG 3 cut(s) 450, 586, 1314
Cac8I GCNNGC 7 cut(s) 234, 514, 602, 803, 920, 1305, 1434
CaiI CAGNNNCTG 1 cut(s) 1303
CfoI GCGC 1 cut(s) 1438
Cfr13I GGNCC 2 cut(s) 238, 305
CseI GACGC 1 cut(s) 128
Csp6I GTAC 4 cut(s) 440, 526, 719, 1135
CviAII CATG 8 cut(s) 417, 513, 802, 841, 919, 985, 1255, 1429
CviQI GTAC 4 cut(s) 440, 526, 719, 1135
DdeI CTNAG 4 cut(s) 222, 503, 753, 1326
DpnI GATC 6 cut(s) 483, 501, 537, 750, 915, 1267
DpnII GATC 6 cut(s) 481, 499, 535, 748, 913, 1265
DraI TTTAAA 1 cut(s) 298
Eam1104I CTCTTC 4 cut(s) 13, 21, 1036, 1125
EarI CTCTTC 4 cut(s) 13, 21, 1036, 1125
Eco130I CCWWGG 3 cut(s) 333, 416, 1046
Eco32I GATATC 1 cut(s) 466
Eco47I GGWCC 2 cut(s) 238, 305
Eco47III AGCGCT 1 cut(s) 1437
Eco57I CTGAAG 3 cut(s) 222, 228, 1149
Eco91I GGTNACC 1 cut(s) 84
EcoO65I GGTNACC 1 cut(s) 84
EcoRI GAATTC 1 cut(s) 138
EcoRV GATATC 1 cut(s) 466
EcoT14I CCWWGG 3 cut(s) 333, 416, 1046
EcoT22I ATGCAT 1 cut(s) 797
ErhI CCWWGG 3 cut(s) 333, 416, 1046
FaeI CATG 8 cut(s) 420, 516, 805, 844, 922, 988, 1258, 1432
FaqI GGGAC 1 cut(s) 92
FatI CATG 8 cut(s) 416, 512, 801, 840, 918, 984, 1254, 1428
FauNDI CATATG 1 cut(s) 853
Fnu4HI GCNGC 4 cut(s) 162, 1200, 1203, 1383
FokI GGATG 6 cut(s) 93, 350, 433, 438, 1064, 1247
Fsp4HI GCNGC 4 cut(s) 162, 1200, 1203, 1383
FspBI CTAG 6 cut(s) 360, 383, 608, 612, 1346, 1365
GlaI GCGC 1 cut(s) 1437
GluI GCNGC 4 cut(s) 162, 1200, 1203, 1383
GsaI CCCAGC 1 cut(s) 600
HaeII RGCGCY 1 cut(s) 1439
HaeIII GGCC 1 cut(s) 5
HapII CCGG 2 cut(s) 355, 1068
HgaI GACGC 1 cut(s) 128
HhaI GCGC 1 cut(s) 1438
Hin1II CATG 8 cut(s) 420, 516, 805, 844, 922, 988, 1258, 1432
Hin6I GCGC 1 cut(s) 1436
HinP1I GCGC 1 cut(s) 1436
HincII GTYRAC 1 cut(s) 396
HindII GTYRAC 1 cut(s) 396
HinfI GANTC 6 cut(s) 640, 759, 1042, 1057, 1091, 1349
HpaI GTTAAC 1 cut(s) 396
HpaII CCGG 2 cut(s) 355, 1068
HphI GGTGA 5 cut(s) 20, 78, 578, 974, 1026
Hpy166II GTNNAC 5 cut(s) 396, 719, 821, 992, 1218
Hpy188I TCNGA 6 cut(s) 223, 504, 631, 1228, 1360, 1469
Hpy8I GTNNAC 5 cut(s) 396, 719, 821, 992, 1218
Hpy99I CGWCG 1 cut(s) 681
HpyAV CCTTC 5 cut(s) 203, 296, 527, 1105, 1417
HpyCH4III ACNGT 3 cut(s) 106, 400, 976
HpyCH4IV ACGT 2 cut(s) 676, 1078
HpyF10VI GCNNNNNNNGC 3 cut(s) 161, 170, 1196
HpyF3I CTNAG 4 cut(s) 222, 503, 753, 1326
HpySE526I ACGT 2 cut(s) 676, 1078
Hsp92II CATG 8 cut(s) 420, 516, 805, 844, 922, 988, 1258, 1432
HspAI GCGC 1 cut(s) 1436
Kpn2I TCCGGA 1 cut(s) 1067
KspAI GTTAAC 1 cut(s) 396
Kzo9I GATC 6 cut(s) 481, 499, 535, 748, 913, 1265
LmnI GCTCC 5 cut(s) 178, 229, 417, 622, 1187
Lsp1109I GCAGC 3 cut(s) 173, 1211, 1369
LweI GCATC 6 cut(s) 34, 782, 823, 1002, 1290, 1351
MaeI CTAG 6 cut(s) 360, 383, 608, 612, 1346, 1365
MaeII ACGT 2 cut(s) 676, 1078
MaeIII GTNAC 3 cut(s) 84, 962, 1278
MalI GATC 6 cut(s) 483, 501, 537, 750, 915, 1267
MbiI CCGCTC 1 cut(s) 1456
MboI GATC 6 cut(s) 481, 499, 535, 748, 913, 1265
MboII GAAGA 7 cut(s) 3, 8, 554, 1023, 1142, 1275, 1298
MflI RGATCY 2 cut(s) 499, 1265
MhlI GDGCHC 2 cut(s) 596, 823
MmeI TCCRAC 2 cut(s) 96, 207
Mph1103I ATGCAT 1 cut(s) 797
MroI TCCGGA 1 cut(s) 1067
MroXI GAANNNNTTC 2 cut(s) 348, 1412
MseI TTAA 7 cut(s) 150, 297, 395, 471, 732, 909, 954
MslI CAYNNNNRTG 3 cut(s) 714, 800, 806
MspA1I CMGCKG 2 cut(s) 236, 1202
MspI CCGG 2 cut(s) 355, 1068
MspR9I CCNGG 1 cut(s) 356
Mva1269I GAATGC 2 cut(s) 132, 1295
MwoI GCNNNNNNNGC 3 cut(s) 161, 170, 1196
NciI CCSGG 1 cut(s) 356
NcoI CCATGG 1 cut(s) 416
NdeI CATATG 1 cut(s) 853
NdeII GATC 6 cut(s) 481, 499, 535, 748, 913, 1265
NlaIII CATG 8 cut(s) 420, 516, 805, 844, 922, 988, 1258, 1432
NlaIV GGNNCC 3 cut(s) 70, 231, 240
NmuCI GTSAC 2 cut(s) 84, 962
NsiI ATGCAT 1 cut(s) 797
NspI RCATGY 4 cut(s) 516, 805, 844, 922
NspV TTCGAA 1 cut(s) 136
OliI CACNNNNGTG 1 cut(s) 714
PaeI GCATGC 3 cut(s) 516, 805, 922
PcsI WCGNNNNNNNCGW 1 cut(s) 742
PctI GAATGC 2 cut(s) 132, 1295
PdmI GAANNNNTTC 2 cut(s) 348, 1412
PfeI GAWTC 6 cut(s) 640, 759, 1042, 1057, 1091, 1349
PkrI GCNGC 4 cut(s) 163, 1201, 1204, 1384
Ppu21I YACGTR 1 cut(s) 1079
PspEI GGTNACC 1 cut(s) 84
PspFI CCCAGC 1 cut(s) 596
PspN4I GGNNCC 3 cut(s) 70, 231, 240
PspPI GGNCC 2 cut(s) 238, 305
PstI CTGCAG 1 cut(s) 639
PstNI CAGNNNCTG 1 cut(s) 1303
PsuI RGATCY 2 cut(s) 499, 1265
RsaI GTAC 4 cut(s) 441, 527, 720, 1136
RsaNI GTAC 4 cut(s) 440, 526, 719, 1135
RseI CAYNNNNRTG 3 cut(s) 714, 800, 806
SaqAI TTAA 7 cut(s) 150, 297, 395, 471, 732, 909, 954
SatI GCNGC 4 cut(s) 162, 1200, 1203, 1383
Sau3AI GATC 6 cut(s) 481, 499, 535, 748, 913, 1265
Sau96I GGNCC 2 cut(s) 238, 305
ScaI AGTACT 1 cut(s) 1136
ScrFI CCNGG 1 cut(s) 356
SduI GDGCHC 2 cut(s) 596, 823
SfaNI GCATC 6 cut(s) 34, 782, 823, 1002, 1290, 1351
SfcI CTRYAG 1 cut(s) 635
SfuI TTCGAA 1 cut(s) 136
SinI GGWCC 2 cut(s) 238, 305
SmiMI CAYNNNNRTG 3 cut(s) 714, 800, 806
SphI GCATGC 3 cut(s) 516, 805, 922
SsiI CCGC 3 cut(s) 236, 1202, 1456
SspI AATATT 1 cut(s) 459
SspMI CTAG 6 cut(s) 360, 383, 608, 612, 1346, 1365
StyD4I CCNGG 1 cut(s) 354
StyI CCWWGG 3 cut(s) 333, 416, 1046
TaaI ACNGT 3 cut(s) 106, 400, 976
TaiI ACGT 2 cut(s) 679, 1081
TaqI TCGA 3 cut(s) 136, 679, 1352
TatI WGTACW 1 cut(s) 1134
TauI GCSGC 1 cut(s) 1205
TfiI GAWTC 6 cut(s) 640, 759, 1042, 1057, 1091, 1349
Tru1I TTAA 7 cut(s) 150, 297, 395, 471, 732, 909, 954
Tru9I TTAA 7 cut(s) 150, 297, 395, 471, 732, 909, 954
TscAI CASTG 3 cut(s) 450, 586, 1314
TseFI GTSAC 2 cut(s) 84, 962
TseI GCWGC 3 cut(s) 161, 1199, 1382
Tsp45I GTSAC 2 cut(s) 84, 962
TspDTI ATGAA 2 cut(s) 751, 1073
TspGWI ACGGA 2 cut(s) 168, 964
TspRI CASTG 3 cut(s) 450, 586, 1314
VneI GTGCAC 1 cut(s) 819
VpaK11BI GGWCC 2 cut(s) 238, 305
XapI RAATTY 5 cut(s) 138, 293, 375, 689, 1341
XbaI TCTAGA 1 cut(s) 1345
XceI RCATGY 4 cut(s) 516, 805, 844, 922
XcmI CCANNNNNNNNNTGG 1 cut(s) 492
XmnI GAANNNNTTC 2 cut(s) 348, 1412
XspI CTAG 6 cut(s) 360, 383, 608, 612, 1346, 1365
ZrmI AGTACT 1 cut(s) 1136
Zsp2I ATGCAT 1 cut(s) 797
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.