Rorug05G0066800

Ribulose-1,5 bisphosphate carboxylase oxygenase large subunit N-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0011165
Physical Location & Seq
Reverse (-)
5741772 .. 5743773
2002 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0066800.1

Sequence Viewer

Length: 1086 bp
ATGGTTTTGATTCATATTCTTTCTTTGCAGGTGAGACTAGGTGAGATCTTGGAGGATGGTCAAGTTGTTGTAAAAAGCTTGGCAACAAACTGTCAGTGTGTGTATAGGCTTGCTGTCAAGCCTGATATTCCCCACATACTGTATAGCTGTAGTGAGGATGGTTTGATTCGACAATTTGATCTGAGAAGCAACTCTGTTATGGAGCTACTTCGTTGCACCCCATTATTGACAAAACAGAAGCACATCAAAGAGAGGGTAGAGGTGTATGCCATGGTATTTGACCCAAGTGATCCAAATTACTTTTGCATCGGAGCTGATGATGTCTATGCAATTGTCTATGACATCAGAAATTTGCAGCGCGATGCGCCAAGGAATTTAGATAAACCAGTGGACACATTTTGCCCTAACCACTTGATTGAGAGAAATGGTGTTCACATCACAGGGTTGGCTTACGCTAATTCAAACTCAAGTGAGCTACTTGTGACTTATTATAATGAGCATATATATCTATTCCAGAAGAATATGGGGTTGGGTCCCTCGCCATCTTCTGCGTTATCCAAAGACATACTGAAGCTTGAGAAGCCACAAGTTTTCTTGGGAAATAGAAATGCATGGACACCCAGAGCAGTGAGCTTCTTTGGCCCCAATGATGATTATGTCTTGAGTGGGAGTGATTGTGGTCATATATATATATATTTGGAAGAAGAAAGGAGGTGTGTAAAGCTCTGGGGTCCTATGGCAAATGATGCTCTACCACCGCCTTACCCACACAACATAGAAAAGAGTTCGCTCGTTGCTCCCATTCGTAGTGTAGAGCTTTGTTTCCTGATGTCACTCTTGAAGATGGAAGACAATTTGCCGGAAATTCGAAGAACTGTAACTGAGATTCAACCAAGTAGTTATGTTCGATTCCCTGATTGTGGGGCTAATATGGGATTTTCGCTTCCAGAGGTTGTACGCCGTGTGCTTATTTGTGACATTGTTTTTGTGATATTATTTGAACAAGCTGAGAGTGTGCGAGCTCTGCCCCTGAATGATGTAACAATAAATTTCTTAGATCATGGTTGCAATATTCACAGGTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rubis-subs-bind SET

Protein Analysis

361

Amino Acids

41.04

Weight (kDa)

5.71

Isoelectric Point (pI)

57.12

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011165)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G20130 AT4G20130 AT4G20130
fragaria_vesca FvH4_3g13130 FvH4_3g13130 FvH4_3g13130 FvH4_3g13130 FvH4_3g13130
malus_domestica MD10G1226200.v1.1
prunus_persica Prupe.4G119100_v2.0.a1 Prupe.4G119100_v2.0.a1
pyrus_communis pycom10g19010
rosa_chinensis RchiOBHm_Chr5g0021381
rosa_laevigata RLG00000032608
rosa_multiflora Rmu_sc0004847.1_g000020
rosa_roxburghii Rroxscaffold_1G00057160
rosa_rugosa Rorug05G0066700 Rorug05G0066800
rosa_samantha Rh5CG169100
rosa_wichuraiana Rw5G013880

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 492
AarI CACCTGC 1 cut(s) 19
Acc36I ACCTGC 1 cut(s) 19
AccII CGCG 1 cut(s) 360
AciI CCGC 1 cut(s) 758
AclWI GGATC 1 cut(s) 284
AcsI RAATTY 4 cut(s) 349, 373, 864, 1048
AcuI CTGAAG 1 cut(s) 590
AfaI GTAC 1 cut(s) 957
AfiI CCNNNNNNNGG 1 cut(s) 920
AgsI TTSAA 4 cut(s) 462, 841, 890, 1001
AjuI GAANNNNNNNTTGG 2 cut(s) 512, 544
Alw21I GWGCWC 1 cut(s) 1024
Alw26I GTCTC 1 cut(s) 28
AlwI GGATC 1 cut(s) 284
AoxI GGCC 1 cut(s) 640
ApeKI GCWGC 1 cut(s) 355
ApoI RAATTY 4 cut(s) 349, 373, 864, 1048
ArsI GACNNNNNNTTYG 2 cut(s) 968, 1000
AspLEI GCGC 2 cut(s) 360, 367
AspS9I GGNCC 3 cut(s) 533, 641, 731
AsuHPI GGTGA 2 cut(s) 43, 53
AsuII TTCGAA 1 cut(s) 868
AvaII GGWCC 2 cut(s) 533, 731
BanII GRGCYC 1 cut(s) 1024
BbsI GAAGAC 1 cut(s) 855
Bbv12I GWGCWC 1 cut(s) 1024
BbvI GCAGC 1 cut(s) 367
BccI CCATC 4 cut(s) 50, 152, 550, 838
BceAI ACGGC 1 cut(s) 945
BcoDI GTCTC 1 cut(s) 28
BfaI CTAG 1 cut(s) 38
BfmI CTRYAG 1 cut(s) 148
BfuAI ACCTGC 1 cut(s) 19
BglII AGATCT 1 cut(s) 45
BisI GCNGC 1 cut(s) 356
BlsI GCNGC 1 cut(s) 357
Bme18I GGWCC 2 cut(s) 533, 731
BmgT120I GGNCC 3 cut(s) 533, 641, 731
BmiI GGNNCC 4 cut(s) 534, 535, 643, 732
BmsI GCATC 3 cut(s) 315, 352, 736
BpiI GAAGAC 1 cut(s) 855
Bpu14I TTCGAA 1 cut(s) 868
BpuEI CTTGAG 3 cut(s) 451, 596, 682
BsaJI CCNNGG 2 cut(s) 270, 368
BsaXI ACNNNNNCTCC 2 cut(s) 661, 691
Bsc4I CCNNNNNNNGG 1 cut(s) 920
Bse1I ACTGG 1 cut(s) 386
BseDI CCNNGG 2 cut(s) 270, 368
BseGI GGATG 2 cut(s) 61, 163
BseLI CCNNNNNNNGG 1 cut(s) 920
BseMII CTCAG 3 cut(s) 173, 873, 999
BseNI ACTGG 1 cut(s) 386
BseXI GCAGC 1 cut(s) 367
Bsh1236I CGCG 1 cut(s) 360
BshFI GGCC 1 cut(s) 642
BsiHKAI GWGCWC 1 cut(s) 1024
BsiSI CCGG 1 cut(s) 860
BslFI GGGAC 1 cut(s) 519
BslI CCNNNNNNNGG 1 cut(s) 920
BsmAI GTCTC 1 cut(s) 28
BsmFI GGGAC 1 cut(s) 519
BsnI GGCC 1 cut(s) 642
Bsp119I TTCGAA 1 cut(s) 868
Bsp1286I GDGCHC 1 cut(s) 1024
Bsp143I GATC 4 cut(s) 45, 178, 289, 1057
Bsp19I CCATGG 1 cut(s) 270
BspACI CCGC 1 cut(s) 758
BspANI GGCC 1 cut(s) 642
BspCNI CTCAG 3 cut(s) 174, 874, 1000
BspFNI CGCG 1 cut(s) 360
BspLI GGNNCC 4 cut(s) 534, 535, 643, 732
BspMI ACCTGC 1 cut(s) 19
BspPI GGATC 1 cut(s) 284
BspT104I TTCGAA 1 cut(s) 868
BsrI ACTGG 1 cut(s) 386
BssECI CCNNGG 2 cut(s) 270, 368
BssMI GATC 4 cut(s) 45, 178, 289, 1057
BssT1I CCWWGG 2 cut(s) 270, 368
Bst4CI ACNGT 3 cut(s) 92, 141, 877
BstAPI GCANNNNNTGC 1 cut(s) 746
BstBI TTCGAA 1 cut(s) 868
BstC8I GCNNGC 2 cut(s) 111, 1020
BstDEI CTNAG 4 cut(s) 182, 882, 1008, 1054
BstDSI CCRYGG 1 cut(s) 270
BstF5I GGATG 2 cut(s) 61, 163
BstFNI CGCG 1 cut(s) 360
BstHHI GCGC 2 cut(s) 360, 367
BstKTI GATC 4 cut(s) 48, 181, 292, 1060
BstMAI GTCTC 1 cut(s) 28
BstMBI GATC 4 cut(s) 45, 178, 289, 1057
BstMWI GCNNNNNNNGC 5 cut(s) 364, 580, 639, 746, 1024
BstSFI CTRYAG 1 cut(s) 148
BstUI CGCG 1 cut(s) 360
BstV1I GCAGC 1 cut(s) 367
BstV2I GAAGAC 1 cut(s) 855
BstX2I RGATCY 1 cut(s) 45
BstYI RGATCY 1 cut(s) 45
BsuRI GGCC 1 cut(s) 642
BtgI CCRYGG 1 cut(s) 270
BtgZI GCGATG 1 cut(s) 375
BtsCI GGATG 2 cut(s) 61, 163
BtsI GCAGTG 1 cut(s) 633
BtsIMutI CAGTG 3 cut(s) 101, 393, 633
BveI ACCTGC 1 cut(s) 19
Cac8I GCNNGC 2 cut(s) 111, 1020
CfoI GCGC 2 cut(s) 360, 367
Cfr13I GGNCC 3 cut(s) 533, 641, 731
Csp6I GTAC 1 cut(s) 956
CviAII CATG 3 cut(s) 271, 612, 1061
CviQI GTAC 1 cut(s) 956
DdeI CTNAG 4 cut(s) 182, 882, 1008, 1054
DpnI GATC 4 cut(s) 47, 180, 291, 1059
DpnII GATC 4 cut(s) 45, 178, 289, 1057
Ecl136II GAGCTC 1 cut(s) 1022
Eco130I CCWWGG 2 cut(s) 270, 368
Eco24I GRGCYC 1 cut(s) 1024
Eco47I GGWCC 2 cut(s) 533, 731
Eco53kI GAGCTC 1 cut(s) 1022
Eco57I CTGAAG 1 cut(s) 590
EcoICRI GAGCTC 1 cut(s) 1022
EcoO109I RGGNCCY 2 cut(s) 533, 731
EcoT14I CCWWGG 2 cut(s) 270, 368
EcoT22I ATGCAT 1 cut(s) 613
EcoT38I GRGCYC 1 cut(s) 1024
ErhI CCWWGG 2 cut(s) 270, 368
FaeI CATG 3 cut(s) 274, 615, 1064
FaqI GGGAC 1 cut(s) 519
FatI CATG 3 cut(s) 270, 611, 1060
Fnu4HI GCNGC 1 cut(s) 356
FokI GGATG 2 cut(s) 68, 170
FriOI GRGCYC 1 cut(s) 1024
Fsp4HI GCNGC 1 cut(s) 356
FspBI CTAG 1 cut(s) 38
GlaI GCGC 2 cut(s) 359, 366
GluI GCNGC 1 cut(s) 356
HaeIII GGCC 1 cut(s) 642
HapII CCGG 1 cut(s) 860
HhaI GCGC 2 cut(s) 360, 367
Hin1II CATG 3 cut(s) 274, 615, 1064
Hin6I GCGC 2 cut(s) 358, 365
HinP1I GCGC 2 cut(s) 358, 365
HindIII AAGCTT 2 cut(s) 76, 572
HinfI GANTC 4 cut(s) 10, 166, 886, 909
HpaII CCGG 1 cut(s) 860
HphI GGTGA 2 cut(s) 43, 53
Hpy166II GTNNAC 2 cut(s) 391, 433
Hpy188I TCNGA 3 cut(s) 183, 311, 347
Hpy188III TCNNGA 5 cut(s) 514, 661, 826, 838, 947
Hpy8I GTNNAC 2 cut(s) 391, 433
HpyCH4III ACNGT 3 cut(s) 92, 141, 877
HpyCH4V TGCA 7 cut(s) 28, 216, 306, 329, 355, 611, 1068
HpyF10VI GCNNNNNNNGC 5 cut(s) 364, 580, 639, 746, 1024
HpyF3I CTNAG 4 cut(s) 182, 882, 1008, 1054
Hsp92II CATG 3 cut(s) 274, 615, 1064
HspAI GCGC 2 cut(s) 358, 365
KflI GGGWCCC 1 cut(s) 533
Kzo9I GATC 4 cut(s) 45, 178, 289, 1057
LmnI GCTCC 3 cut(s) 202, 311, 802
Lsp1109I GCAGC 1 cut(s) 367
LweI GCATC 3 cut(s) 315, 352, 736
MaeI CTAG 1 cut(s) 38
MaeIII GTNAC 5 cut(s) 481, 831, 877, 974, 1039
MalI GATC 4 cut(s) 47, 180, 291, 1059
MboI GATC 4 cut(s) 45, 178, 289, 1057
MboII GAAGA 7 cut(s) 529, 537, 713, 716, 853, 860, 882
MfeI CAATTG 1 cut(s) 330
MflI RGATCY 1 cut(s) 45
MhlI GDGCHC 1 cut(s) 1024
MluCI AATT 9 cut(s) 173, 295, 330, 349, 373, 457, 853, 864, 1048
MnlI CCTC 7 cut(s) 46, 148, 246, 253, 547, 705, 943
Mph1103I ATGCAT 1 cut(s) 613
MspI CCGG 1 cut(s) 860
MunI CAATTG 1 cut(s) 330
MvnI CGCG 1 cut(s) 360
MwoI GCNNNNNNNGC 5 cut(s) 364, 580, 639, 746, 1024
NcoI CCATGG 1 cut(s) 270
NdeII GATC 4 cut(s) 45, 178, 289, 1057
NlaIII CATG 3 cut(s) 274, 615, 1064
NlaIV GGNNCC 4 cut(s) 534, 535, 643, 732
NmuCI GTSAC 3 cut(s) 481, 831, 974
NsiI ATGCAT 1 cut(s) 613
NspV TTCGAA 1 cut(s) 868
PaqCI CACCTGC 1 cut(s) 19
PfeI GAWTC 4 cut(s) 10, 166, 886, 909
PkrI GCNGC 1 cut(s) 357
PpuMI RGGWCCY 2 cut(s) 533, 731
PsiI TTATAA 1 cut(s) 492
Psp124BI GAGCTC 1 cut(s) 1024
Psp5II RGGWCCY 2 cut(s) 533, 731
PspN4I GGNNCC 4 cut(s) 534, 535, 643, 732
PspPI GGNCC 3 cut(s) 533, 641, 731
PspPPI RGGWCCY 2 cut(s) 533, 731
PsuI RGATCY 1 cut(s) 45
RsaI GTAC 1 cut(s) 957
RsaNI GTAC 1 cut(s) 956
SacI GAGCTC 1 cut(s) 1024
SatI GCNGC 1 cut(s) 356
Sau3AI GATC 4 cut(s) 45, 178, 289, 1057
Sau96I GGNCC 3 cut(s) 533, 641, 731
SduI GDGCHC 1 cut(s) 1024
SfaNI GCATC 3 cut(s) 315, 352, 736
SfcI CTRYAG 1 cut(s) 148
SfuI TTCGAA 1 cut(s) 868
SinI GGWCC 2 cut(s) 533, 731
SmlI CTYRAG 3 cut(s) 466, 575, 661
SmoI CTYRAG 3 cut(s) 466, 575, 661
Sse9I AATT 9 cut(s) 173, 295, 330, 349, 373, 457, 853, 864, 1048
SsiI CCGC 1 cut(s) 758
SspI AATATT 1 cut(s) 1072
SspMI CTAG 1 cut(s) 38
SstI GAGCTC 1 cut(s) 1024
StyI CCWWGG 2 cut(s) 270, 368
TaaI ACNGT 3 cut(s) 92, 141, 877
TaqI TCGA 3 cut(s) 169, 868, 907
TasI AATT 9 cut(s) 173, 295, 330, 349, 373, 457, 853, 864, 1048
TfiI GAWTC 4 cut(s) 10, 166, 886, 909
TscAI CASTG 3 cut(s) 101, 393, 633
TseFI GTSAC 3 cut(s) 481, 831, 974
TseI GCWGC 1 cut(s) 355
Tsp45I GTSAC 3 cut(s) 481, 831, 974
TspRI CASTG 3 cut(s) 101, 393, 633
VpaK11BI GGWCC 2 cut(s) 533, 731
XapI RAATTY 4 cut(s) 349, 373, 864, 1048
XspI CTAG 1 cut(s) 38
Zsp2I ATGCAT 1 cut(s) 613
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.