Rh5CG169100

Ribulose-1,5 bisphosphate carboxylase oxygenase large subunit N-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0011165
Physical Location & Seq
Reverse (-)
15768402 .. 15771962
3561 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG169100.1

Sequence Viewer

Length: 1194 bp
ATGAGAGCTTATGGAATTGAATTTAAAGAAGGCCCAGATGGGTTTGGAGTTTATGCTTCCAAGGATGTTGAACCACTTCGCCGTGCTAGGGTGATAATGGAAATACCACTAGAACTGATGTTAACTATAAGCCAAAAGCTCCCATGGATGTTTTTCCCAGATATAGTACCAGTGGGTCACCCAATATTTGATATCATCAATTCAACTGATCCAGAGACAGGTTGGGATCTAAGGTTAGCTTGCCTTCTTTTGTATGCATTTGATCGGGAGGATAACTTTTGGCAGTTGTATAGTGATTTTTTACCCAGTGCAGATGAGTGCCCCAGTCTGCTTTTAGCTAGCGAGGAGGAGCTTTCAGAACTACAGGATCCCAATCTTGCTTCAACAATGAGAGAACAGCAATGCCGAGCTCTGGAATTTTGGGAGAAAAATTGGCATTCTGGTGTACCCCTTAAGATTAAACGCCTCGCTCGAGATTCTGAGAGATTCATCTGGGCACTGAGTATGGCACAATCACGATGCATTGGCATGCAAATTAGAATTGGTGCACTTTTTCAAGACTCAAATATGCTAATTCCTTATGCAGACATGCTAAACCATTCCTTCCAGCCAAATTGTTTTCTCCATTGGCGGTTTAAGGATCGTATGCTTGAAGTAATGATAAATGCTGGACAACGGATTAAGAAAGGTGATGAGATGACTATCAATTACATGAGTGGACAACAGAACAACATGTTGATGCAAAGATATGGCTTTTCTTCATCTGTGAATCCATGGGATGTAATTCAATTCTCTGGAAATGCACGTATTCATTTGGATTCCTTCTTGTCAGTATTCAATATCTCAGGACTTCCCCAAGAATACTATCATAACAATCGCCTATCTAGTGGAGGGGATACTTTTGTTGACGGAGCTGTCATCGCAGCAGCAAGAACATTGCCTACTTGGTCAGATGGGGATGTGCCTCCAATCCCAAGCATGGAAAGACGAGCGATAAAGGAATTACAGGAAGAATGCAGACAGATGCTTGTCAAATTTCCTACCAACTCTAAGGAAGACCAAAATATCCTAGATTCTATGCCAGACGCTACGAGGACGCTTGAAGCTGTGGTCAAGTATAGGTTGCACCGGAAGTTGTTCATCGAGAAAGTCATGCAGACACTGGATCTCTATCAAGAACGAATATTATTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rubis-subs-bind SET

Protein Analysis

397

Amino Acids

46.01

Weight (kDa)

5.26

Isoelectric Point (pI)

47.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SET PF00856 14 - 237 2e-10 SET domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011165)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G20130 AT4G20130 AT4G20130
fragaria_vesca FvH4_3g13130 FvH4_3g13130 FvH4_3g13130 FvH4_3g13130 FvH4_3g13130
malus_domestica MD10G1226200.v1.1
prunus_persica Prupe.4G119100_v2.0.a1 Prupe.4G119100_v2.0.a1
pyrus_communis pycom10g19010
rosa_chinensis RchiOBHm_Chr5g0021381
rosa_laevigata RLG00000032608
rosa_multiflora Rmu_sc0004847.1_g000020
rosa_roxburghii Rroxscaffold_1G00057160
rosa_rugosa Rorug05G0066700 Rorug05G0066800
rosa_samantha Rh5CG169100
rosa_wichuraiana Rw5G013880

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 914
AciI CCGC 1 cut(s) 631
AclWI GGATC 6 cut(s) 203, 234, 362, 375, 648, 1173
AcsI RAATTY 3 cut(s) 20, 416, 1034
AfaI GTAC 2 cut(s) 168, 447
AfiI CCNNNNNNNGG 4 cut(s) 88, 218, 412, 979
AflII CTTAAG 1 cut(s) 452
AflIII ACRYGT 1 cut(s) 732
AgsI TTSAA 9 cut(s) 20, 71, 204, 384, 557, 653, 788, 838, 1103
AluBI AGCT 8 cut(s) 8, 139, 239, 338, 352, 410, 914, 1106
AluI AGCT 8 cut(s) 8, 139, 239, 338, 352, 410, 914, 1106
Alw21I GWGCWC 2 cut(s) 412, 550
Alw26I GTCTC 1 cut(s) 209
Alw44I GTGCAC 1 cut(s) 546
AlwI GGATC 6 cut(s) 203, 234, 362, 375, 648, 1173
AlwNI CAGNNNCTG 1 cut(s) 1162
Ama87I CYCGRG 1 cut(s) 471
AoxI GGCC 1 cut(s) 31
ApaLI GTGCAC 1 cut(s) 546
ApeKI GCWGC 2 cut(s) 923, 926
ApoI RAATTY 3 cut(s) 20, 416, 1034
Asp700I GAANNNNTTC 2 cut(s) 75, 1136
AspS9I GGNCC 1 cut(s) 32
AsuHPI GGTGA 3 cut(s) 103, 170, 701
AsuNHI GCTAGC 1 cut(s) 338
AvaI CYCGRG 1 cut(s) 471
BaeGI GKGCMC 3 cut(s) 323, 499, 550
BaeI ACNNNNGTAYC 2 cut(s) 888, 921
BamHI GGATCC 1 cut(s) 367
BanII GRGCYC 1 cut(s) 412
BbsI GAAGAC 1 cut(s) 1062
Bbv12I GWGCWC 2 cut(s) 412, 550
BbvI GCAGC 2 cut(s) 935, 938
BccI CCATC 2 cut(s) 32, 947
BceAI ACGGC 1 cut(s) 66
BciVI GTATCC 1 cut(s) 889
BcoDI GTCTC 1 cut(s) 209
BfaI CTAG 5 cut(s) 87, 110, 339, 885, 1070
BfmI CTRYAG 1 cut(s) 362
BfrI CTTAAG 1 cut(s) 452
BfuI GTATCC 1 cut(s) 889
BisI GCNGC 2 cut(s) 924, 927
BlsI GCNGC 2 cut(s) 925, 928
BmeT110I CYCGRG 1 cut(s) 471
BmgT120I GGNCC 1 cut(s) 32
BmiI GGNNCC 1 cut(s) 369
BmrI ACTGGG 2 cut(s) 300, 318
BmsI GCATC 3 cut(s) 509, 729, 1014
BmtI GCTAGC 1 cut(s) 342
BmuI ACTGGG 2 cut(s) 300, 318
BpiI GAAGAC 1 cut(s) 1062
BsaAI YACGTR 1 cut(s) 806
BsaBI GATNNNNATC 3 cut(s) 372, 701, 1170
BsaJI CCNNGG 3 cut(s) 60, 143, 773
BsaWI WCCGGW 1 cut(s) 1128
Bsc4I CCNNNNNNNGG 4 cut(s) 88, 218, 412, 979
Bse1I ACTGG 4 cut(s) 170, 306, 324, 1167
Bse3DI GCAATG 2 cut(s) 407, 935
Bse8I GATNNNNATC 3 cut(s) 372, 701, 1170
BseDI CCNNGG 3 cut(s) 60, 143, 773
BseGI GGATG 4 cut(s) 70, 153, 784, 964
BseJI GATNNNNATC 3 cut(s) 372, 701, 1170
BseLI CCNNNNNNNGG 4 cut(s) 88, 218, 412, 979
BseMI GCAATG 2 cut(s) 407, 935
BseMII CTCAG 3 cut(s) 471, 491, 858
BseNI ACTGG 4 cut(s) 170, 306, 324, 1167
BseRI GAGGAG 2 cut(s) 359, 362
BseSI GKGCMC 3 cut(s) 323, 499, 550
BseXI GCAGC 2 cut(s) 935, 938
BsgI GTGCAG 1 cut(s) 330
BshFI GGCC 1 cut(s) 33
BsiHKAI GWGCWC 2 cut(s) 412, 550
BsiHKCI CYCGRG 1 cut(s) 471
BsiSI CCGG 1 cut(s) 1129
BslI CCNNNNNNNGG 4 cut(s) 88, 218, 412, 979
BsmAI GTCTC 1 cut(s) 209
BsmI GAATGC 2 cut(s) 436, 1019
BsnI GGCC 1 cut(s) 33
BsoBI CYCGRG 1 cut(s) 471
Bsp1286I GDGCHC 4 cut(s) 323, 412, 499, 550
Bsp143I GATC 6 cut(s) 208, 226, 262, 367, 640, 1165
Bsp19I CCATGG 2 cut(s) 143, 773
BspACI CCGC 1 cut(s) 631
BspANI GGCC 1 cut(s) 33
BspCNI CTCAG 3 cut(s) 472, 492, 857
BspLI GGNNCC 1 cut(s) 369
BspOI GCTAGC 1 cut(s) 342
BspPI GGATC 6 cut(s) 203, 234, 362, 375, 648, 1173
BspTI CTTAAG 1 cut(s) 452
BsrDI GCAATG 2 cut(s) 407, 935
BsrI ACTGG 4 cut(s) 170, 306, 324, 1167
BssECI CCNNGG 3 cut(s) 60, 143, 773
BssMI GATC 6 cut(s) 208, 226, 262, 367, 640, 1165
BssT1I CCWWGG 3 cut(s) 60, 143, 773
BstAFI CTTAAG 1 cut(s) 452
BstBAI YACGTR 1 cut(s) 806
BstC8I GCNNGC 3 cut(s) 241, 340, 530
BstDEI CTNAG 5 cut(s) 230, 480, 500, 844, 1050
BstDSI CCRYGG 2 cut(s) 143, 773
BstEII GGTNACC 1 cut(s) 176
BstF5I GGATG 4 cut(s) 70, 153, 784, 964
BstKTI GATC 6 cut(s) 211, 229, 265, 370, 643, 1168
BstMAI GTCTC 1 cut(s) 209
BstMBI GATC 6 cut(s) 208, 226, 262, 367, 640, 1165
BstMWI GCNNNNNNNGC 1 cut(s) 920
BstNSI RCATGY 3 cut(s) 532, 592, 736
BstPI GGTNACC 1 cut(s) 176
BstSFI CTRYAG 1 cut(s) 362
BstSLI GKGCMC 3 cut(s) 323, 499, 550
BstV1I GCAGC 2 cut(s) 935, 938
BstV2I GAAGAC 1 cut(s) 1062
BstX2I RGATCY 3 cut(s) 226, 367, 1165
BstYI RGATCY 3 cut(s) 226, 367, 1165
BsuI GTATCC 1 cut(s) 889
BsuRI GGCC 1 cut(s) 33
BtgI CCRYGG 2 cut(s) 143, 773
BtgZI GCGATG 1 cut(s) 904
BtsCI GGATG 4 cut(s) 70, 153, 784, 964
BtsIMutI CAGTG 4 cut(s) 177, 313, 497, 1160
Cac8I GCNNGC 3 cut(s) 241, 340, 530
CaiI CAGNNNCTG 1 cut(s) 1162
Cfr13I GGNCC 1 cut(s) 32
CseI GACGC 2 cut(s) 1094, 1105
Csp6I GTAC 2 cut(s) 167, 446
CviAII CATG 8 cut(s) 144, 529, 589, 712, 733, 774, 979, 1153
CviQI GTAC 2 cut(s) 167, 446
DdeI CTNAG 5 cut(s) 230, 480, 500, 844, 1050
DpnI GATC 6 cut(s) 210, 228, 264, 369, 642, 1167
DpnII GATC 6 cut(s) 208, 226, 262, 367, 640, 1165
DraI TTTAAA 1 cut(s) 25
DrdI GACNNNNNNGTC 1 cut(s) 914
DseDI GACNNNNNNGTC 1 cut(s) 914
Ecl136II GAGCTC 1 cut(s) 410
Eco130I CCWWGG 3 cut(s) 60, 143, 773
Eco24I GRGCYC 1 cut(s) 412
Eco32I GATATC 1 cut(s) 193
Eco53kI GAGCTC 1 cut(s) 410
Eco88I CYCGRG 1 cut(s) 471
Eco91I GGTNACC 1 cut(s) 176
EcoICRI GAGCTC 1 cut(s) 410
EcoO65I GGTNACC 1 cut(s) 176
EcoRV GATATC 1 cut(s) 193
EcoT14I CCWWGG 3 cut(s) 60, 143, 773
EcoT22I ATGCAT 2 cut(s) 259, 524
EcoT38I GRGCYC 1 cut(s) 412
ErhI CCWWGG 3 cut(s) 60, 143, 773
FaeI CATG 8 cut(s) 147, 532, 592, 715, 736, 777, 982, 1156
FalI AAGNNNNNCTT 2 cut(s) 223, 255
FatI CATG 8 cut(s) 143, 528, 588, 711, 732, 773, 978, 1152
Fnu4HI GCNGC 2 cut(s) 924, 927
FokI GGATG 4 cut(s) 77, 160, 791, 971
FriOI GRGCYC 1 cut(s) 412
Fsp4HI GCNGC 2 cut(s) 924, 927
FspBI CTAG 5 cut(s) 87, 110, 339, 885, 1070
GluI GCNGC 2 cut(s) 924, 927
HaeIII GGCC 1 cut(s) 33
HapII CCGG 1 cut(s) 1129
HgaI GACGC 2 cut(s) 1094, 1105
Hin1II CATG 8 cut(s) 147, 532, 592, 715, 736, 777, 982, 1156
HincII GTYRAC 2 cut(s) 123, 907
HindII GTYRAC 2 cut(s) 123, 907
HinfI GANTC 6 cut(s) 476, 486, 560, 769, 818, 1073
HpaI GTTAAC 1 cut(s) 123
HpaII CCGG 1 cut(s) 1129
HphI GGTGA 3 cut(s) 103, 170, 701
Hpy166II GTNNAC 5 cut(s) 123, 446, 548, 719, 907
Hpy188I TCNGA 4 cut(s) 358, 481, 952, 1193
Hpy8I GTNNAC 5 cut(s) 123, 446, 548, 719, 907
HpyAV CCTTC 4 cut(s) 23, 254, 613, 832
HpyCH4IV ACGT 1 cut(s) 805
HpyF10VI GCNNNNNNNGC 1 cut(s) 920
HpyF3I CTNAG 5 cut(s) 230, 480, 500, 844, 1050
HpySE526I ACGT 1 cut(s) 805
Hsp92II CATG 8 cut(s) 147, 532, 592, 715, 736, 777, 982, 1156
KspAI GTTAAC 1 cut(s) 123
Kzo9I GATC 6 cut(s) 208, 226, 262, 367, 640, 1165
LmnI GCTCC 3 cut(s) 144, 349, 911
Lsp1109I GCAGC 2 cut(s) 935, 938
LweI GCATC 3 cut(s) 509, 729, 1014
MaeI CTAG 5 cut(s) 87, 110, 339, 885, 1070
MaeII ACGT 1 cut(s) 805
MaeIII GTNAC 1 cut(s) 176
MalI GATC 6 cut(s) 210, 228, 264, 369, 642, 1167
MboI GATC 6 cut(s) 208, 226, 262, 367, 640, 1165
MboII GAAGA 3 cut(s) 750, 1022, 1067
MflI RGATCY 3 cut(s) 226, 367, 1165
MhlI GDGCHC 4 cut(s) 323, 412, 499, 550
MlyI GAGTC 1 cut(s) 554
MnlI CCTC 7 cut(s) 262, 337, 340, 476, 884, 975, 1086
Mph1103I ATGCAT 2 cut(s) 259, 524
MroXI GAANNNNTTC 2 cut(s) 75, 1136
MseI TTAA 6 cut(s) 24, 122, 453, 459, 636, 681
MslI CAYNNNNRTG 3 cut(s) 441, 527, 737
MspCI CTTAAG 1 cut(s) 452
MspI CCGG 1 cut(s) 1129
Mva1269I GAATGC 2 cut(s) 436, 1019
MwoI GCNNNNNNNGC 1 cut(s) 920
NcoI CCATGG 2 cut(s) 143, 773
NdeII GATC 6 cut(s) 208, 226, 262, 367, 640, 1165
NheI GCTAGC 1 cut(s) 338
NlaIII CATG 8 cut(s) 147, 532, 592, 715, 736, 777, 982, 1156
NlaIV GGNNCC 1 cut(s) 369
NmeAIII GCCGAG 1 cut(s) 431
NmuCI GTSAC 1 cut(s) 176
NsiI ATGCAT 2 cut(s) 259, 524
NspI RCATGY 3 cut(s) 532, 592, 736
PaeI GCATGC 1 cut(s) 532
PaeR7I CTCGAG 1 cut(s) 471
PciI ACATGT 1 cut(s) 732
PcsI WCGNNNNNNNCGW 1 cut(s) 469
PctI GAATGC 2 cut(s) 436, 1019
PdmI GAANNNNTTC 2 cut(s) 75, 1136
PfeI GAWTC 5 cut(s) 476, 486, 769, 818, 1073
PkrI GCNGC 2 cut(s) 925, 928
PleI GAGTC 1 cut(s) 554
PpsI GAGTC 1 cut(s) 554
Ppu21I YACGTR 1 cut(s) 806
PscI ACATGT 1 cut(s) 732
Psp124BI GAGCTC 1 cut(s) 412
PspEI GGTNACC 1 cut(s) 176
PspN4I GGNNCC 1 cut(s) 369
PspPI GGNCC 1 cut(s) 32
PsrI GAACNNNNNNTAC 2 cut(s) 925, 957
PstNI CAGNNNCTG 1 cut(s) 1162
PsuI RGATCY 3 cut(s) 226, 367, 1165
RsaI GTAC 2 cut(s) 168, 447
RsaNI GTAC 2 cut(s) 167, 446
RseI CAYNNNNRTG 3 cut(s) 441, 527, 737
SacI GAGCTC 1 cut(s) 412
SaqAI TTAA 6 cut(s) 24, 122, 453, 459, 636, 681
SatI GCNGC 2 cut(s) 924, 927
Sau3AI GATC 6 cut(s) 208, 226, 262, 367, 640, 1165
Sau96I GGNCC 1 cut(s) 32
SchI GAGTC 1 cut(s) 554
SduI GDGCHC 4 cut(s) 323, 412, 499, 550
SfaNI GCATC 3 cut(s) 509, 729, 1014
SfcI CTRYAG 1 cut(s) 362
Sfr274I CTCGAG 1 cut(s) 471
SlaI CTCGAG 1 cut(s) 471
SmiMI CAYNNNNRTG 3 cut(s) 441, 527, 737
SmlI CTYRAG 2 cut(s) 452, 471
SmoI CTYRAG 2 cut(s) 452, 471
SphI GCATGC 1 cut(s) 532
SsiI CCGC 1 cut(s) 631
SspI AATATT 2 cut(s) 186, 1185
SspMI CTAG 5 cut(s) 87, 110, 339, 885, 1070
SstI GAGCTC 1 cut(s) 412
StyI CCWWGG 3 cut(s) 60, 143, 773
TaiI ACGT 1 cut(s) 808
TaqI TCGA 2 cut(s) 472, 1143
TfiI GAWTC 5 cut(s) 476, 486, 769, 818, 1073
Tru1I TTAA 6 cut(s) 24, 122, 453, 459, 636, 681
Tru9I TTAA 6 cut(s) 24, 122, 453, 459, 636, 681
TscAI CASTG 4 cut(s) 177, 313, 504, 1167
TseFI GTSAC 1 cut(s) 176
TseI GCWGC 2 cut(s) 923, 926
Tsp45I GTSAC 1 cut(s) 176
TspDTI ATGAA 4 cut(s) 478, 750, 800, 1129
TspGWI ACGGA 2 cut(s) 691, 924
TspRI CASTG 4 cut(s) 177, 313, 504, 1167
Vha464I CTTAAG 1 cut(s) 452
VneI GTGCAC 1 cut(s) 546
XapI RAATTY 3 cut(s) 20, 416, 1034
XceI RCATGY 3 cut(s) 532, 592, 736
XcmI CCANNNNNNNNNTGG 1 cut(s) 219
XhoI CTCGAG 1 cut(s) 471
XmnI GAANNNNTTC 2 cut(s) 75, 1136
XspI CTAG 5 cut(s) 87, 110, 339, 885, 1070
Zsp2I ATGCAT 2 cut(s) 259, 524
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.