Rroxscaffold_1G00057160

Ribulose-1,5 bisphosphate carboxylase oxygenase large subunit N-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0011165
Physical Location & Seq
Forward (+)
79154975 .. 79159907
4933 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00057160.1

Sequence Viewer

Length: 1449 bp
ATGGGGAATCATGAGAACGGAGCTGCAAAAGAGGAATACAAGCATCATGCTGTTAACTTGTTAAGGAAGGTCGAGTCACTCGAGTGTTGTATCCTAGTACTCTGTCTCTTCTCTCCAATGGCTTCTTCTCTTCCTCTTCATCACCCTTCCCACTGCCTCATCTCCAATCCTCAGGGCTTCAACTATGGAGGCTCACCAAGACCCAGATTCTCTTTCACCACTAAAGACACAGACAAAGGCATTCGCATTCAACCCATTAAAGCCAAAGCAGCCACCACCGAGGCCTCTCCATTTCCTCTTCTTCAGTCTCCCCGAGCTGAAGACTCTCCCTCTGAGTTGGAGCCAGCAGATCCTGATTTCTACAAGATAGGATTTGTTAGAAGTATGAGAGCTTATGGAATTGAATTTAAAGAAGGCCCAGATGGGTTAGGAGTTTATGCTTCCAAGGATGTTGAACCACTTCGCCGCGCTAGGGTGATAATGGAAATACCACTAGAACTGATGTTAACTATAAGCCAAAAGCTCCCATGGATGTTTTTCCCAGATATAGTACCGGTGGGTCACCCAATATTTGATATCATCAATTCAACTGATCCAGAGGAGGAGCTTTCAGAACTACAGGATCCCAATCTTGCTTCAACAATGAGAGAACAGCAACGCCGAGCTCTGGAATTTTGGGAGAAAAATTGGCATTCTGGTGTACCCCTTAAGATTAAACGCCTGGCTCGTGATTCTGAGAGATTCATTTGGGCACTGAGTATGGCACAATCACGATGCATTGGCATGCAAATTAGAATTGGTGCACTTTTTCAAGACTCAAATATGCTAATTCCTTATGCAGACATGCTAAACCATTCCTTCCAGCCAAATTGTTTTCTCCATTGGCGGTTTAAGGATCGTATGCTTGAAGTAATGATAAATGCTGGACAACGGATTAAGAAGGGTGATGAGATGACTATCAATTACATGAGTGGACAACAGAACAACATGTTGATGCAAAGATATGGCTTTTCTTCATCTGTGAATCCATGGGATGTAATTCAATTCTCTGGAAATGCACGTATTCATTTGGATTCCTTCTTGTCAGTATTCAATATCTCAGGACTTCCCCAAGAATACTATCATAACAATCGCCTATCTAGTGGAGGGGATACTTTTGTTGACGGAGCTGTCATTGCAGCAGCAAGAACATTGCCTACTTGGTCAGATGGGGATGTGCCTCCAATTCCAAGCATGGAAAGACGAGCGATAAAGGAATTACAGGAAGAATGCAGACAGATGCTTGCTGCATTTCCTACCAACTCCAAGGAAGACCAAAATATCCTAGATTCTATGCCAGACGCTACTAGGACGCTTGAAGCTGTGGTCAAGTATAGGTTGCACCGGAAGTTGTTCATCGAGAAAGTCATGCAGGCACTGGATCTATATCAAGAACGAATATTATTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rubis-subs-bind SET

Protein Analysis

482

Amino Acids

55.05

Weight (kDa)

6.26

Isoelectric Point (pI)

51.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SET PF00856 142 - 322 3.3e-10 SET domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011165)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G20130 AT4G20130 AT4G20130
fragaria_vesca FvH4_3g13130 FvH4_3g13130 FvH4_3g13130 FvH4_3g13130 FvH4_3g13130
malus_domestica MD10G1226200.v1.1
prunus_persica Prupe.4G119100_v2.0.a1 Prupe.4G119100_v2.0.a1
pyrus_communis pycom10g19010
rosa_chinensis RchiOBHm_Chr5g0021381
rosa_laevigata RLG00000032608
rosa_multiflora Rmu_sc0004847.1_g000020
rosa_roxburghii Rroxscaffold_1G00057160
rosa_rugosa Rorug05G0066700 Rorug05G0066800
rosa_samantha Rh5CG169100
rosa_wichuraiana Rw5G013880

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1169
AccII CGCG 1 cut(s) 468
AciI CCGC 2 cut(s) 466, 886
AclWI GGATC 6 cut(s) 344, 587, 617, 630, 903, 1428
AcsI RAATTY 2 cut(s) 404, 671
AcuI CTGAAG 2 cut(s) 287, 339
AfaI GTAC 3 cut(s) 99, 552, 702
AfiI CCNNNNNNNGG 2 cut(s) 472, 667
AflII CTTAAG 1 cut(s) 707
AflIII ACRYGT 1 cut(s) 987
AgeI ACCGGT 1 cut(s) 553
AjnI CCWGG 1 cut(s) 720
AjuI GAANNNNNNNTTGG 2 cut(s) 109, 141
AleI CACNNNNGTG 1 cut(s) 82
AluBI AGCT 8 cut(s) 23, 317, 392, 523, 607, 665, 1169, 1361
AluI AGCT 8 cut(s) 23, 317, 392, 523, 607, 665, 1169, 1361
Alw21I GWGCWC 2 cut(s) 667, 805
Alw26I GTCTC 2 cut(s) 110, 312
Alw44I GTGCAC 1 cut(s) 801
AlwI GGATC 6 cut(s) 344, 587, 617, 630, 903, 1428
AlwNI CAGNNNCTG 2 cut(s) 353, 1417
Ama87I CYCGRG 2 cut(s) 80, 312
AoxI GGCC 2 cut(s) 282, 415
ApaLI GTGCAC 1 cut(s) 801
ApeKI GCWGC 5 cut(s) 23, 269, 1178, 1181, 1286
ApoI RAATTY 2 cut(s) 404, 671
AsiGI ACCGGT 1 cut(s) 553
Asp700I GAANNNNTTC 2 cut(s) 459, 1391
AspLEI GCGC 1 cut(s) 470
AspS9I GGNCC 1 cut(s) 416
AsuHPI GGTGA 6 cut(s) 134, 186, 208, 487, 554, 956
AvaI CYCGRG 2 cut(s) 80, 312
AxyI CCTNAGG 1 cut(s) 171
BaeGI GKGCMC 2 cut(s) 754, 805
BaeI ACNNNNGTAYC 2 cut(s) 1143, 1176
BamHI GGATCC 1 cut(s) 622
BanII GRGCYC 1 cut(s) 667
BauI CACGAG 1 cut(s) 726
BbsI GAAGAC 2 cut(s) 327, 1317
Bbv12I GWGCWC 2 cut(s) 667, 805
BbvI GCAGC 5 cut(s) 10, 281, 1190, 1193, 1273
BccI CCATC 2 cut(s) 416, 1202
BciT130I CCWGG 1 cut(s) 722
BciVI GTATCC 2 cut(s) 101, 1144
BcoDI GTCTC 2 cut(s) 110, 312
BfaI CTAG 6 cut(s) 95, 471, 494, 1140, 1325, 1347
BfmI CTRYAG 1 cut(s) 617
BfrI CTTAAG 1 cut(s) 707
BfuI GTATCC 2 cut(s) 101, 1144
BisI GCNGC 6 cut(s) 24, 270, 466, 1179, 1182, 1287
BlsI GCNGC 6 cut(s) 25, 271, 467, 1180, 1183, 1288
BmcAI AGTACT 1 cut(s) 99
Bme1390I CCNGG 1 cut(s) 722
BmeT110I CYCGRG 2 cut(s) 80, 312
BmgT120I GGNCC 1 cut(s) 416
BmiI GGNNCC 2 cut(s) 342, 624
BmrFI CCNGG 1 cut(s) 722
BmsI GCATC 4 cut(s) 52, 764, 984, 1269
BpiI GAAGAC 2 cut(s) 327, 1317
BsaAI YACGTR 1 cut(s) 1061
BsaBI GATNNNNATC 3 cut(s) 627, 956, 1425
BsaJI CCNNGG 5 cut(s) 279, 444, 527, 1028, 1305
BsaWI WCCGGW 2 cut(s) 553, 1383
Bsc4I CCNNNNNNNGG 2 cut(s) 472, 667
Bse118I RCCGGY 1 cut(s) 553
Bse1I ACTGG 1 cut(s) 1422
Bse21I CCTNAGG 1 cut(s) 171
Bse3DI GCAATG 2 cut(s) 1173, 1190
Bse8I GATNNNNATC 3 cut(s) 627, 956, 1425
BseBI CCWGG 1 cut(s) 722
BseDI CCNNGG 5 cut(s) 279, 444, 527, 1028, 1305
BseGI GGATG 4 cut(s) 454, 537, 1039, 1219
BseJI GATNNNNATC 3 cut(s) 627, 956, 1425
BseLI CCNNNNNNNGG 2 cut(s) 472, 667
BseMI GCAATG 2 cut(s) 1173, 1190
BseMII CTCAG 5 cut(s) 185, 324, 726, 746, 1113
BseNI ACTGG 1 cut(s) 1422
BseRI GAGGAG 2 cut(s) 614, 617
BseSI GKGCMC 2 cut(s) 754, 805
BseXI GCAGC 5 cut(s) 10, 281, 1190, 1193, 1273
Bsh1236I CGCG 1 cut(s) 468
BshFI GGCC 2 cut(s) 284, 417
BshTI ACCGGT 1 cut(s) 553
BsiHKAI GWGCWC 2 cut(s) 667, 805
BsiHKCI CYCGRG 2 cut(s) 80, 312
BsiSI CCGG 2 cut(s) 554, 1384
BslI CCNNNNNNNGG 2 cut(s) 472, 667
BsmAI GTCTC 2 cut(s) 110, 312
BsmI GAATGC 4 cut(s) 240, 246, 691, 1274
BsnI GGCC 2 cut(s) 284, 417
BsoBI CYCGRG 2 cut(s) 80, 312
Bsp1286I GDGCHC 3 cut(s) 667, 754, 805
Bsp143I GATC 5 cut(s) 349, 592, 622, 895, 1420
Bsp19I CCATGG 2 cut(s) 527, 1028
BspACI CCGC 2 cut(s) 466, 886
BspANI GGCC 2 cut(s) 284, 417
BspCNI CTCAG 5 cut(s) 184, 325, 727, 747, 1112
BspFNI CGCG 1 cut(s) 468
BspHI TCATGA 1 cut(s) 10
BspLI GGNNCC 2 cut(s) 342, 624
BspPI GGATC 6 cut(s) 344, 587, 617, 630, 903, 1428
BspTI CTTAAG 1 cut(s) 707
BsrDI GCAATG 2 cut(s) 1173, 1190
BsrFI RCCGGY 1 cut(s) 553
BsrI ACTGG 1 cut(s) 1422
BssAI RCCGGY 1 cut(s) 553
BssECI CCNNGG 5 cut(s) 279, 444, 527, 1028, 1305
BssMI GATC 5 cut(s) 349, 592, 622, 895, 1420
BssSI CACGAG 1 cut(s) 726
BssT1I CCWWGG 4 cut(s) 444, 527, 1028, 1305
Bst2BI CACGAG 1 cut(s) 726
Bst2UI CCWGG 1 cut(s) 722
Bst6I CTCTTC 4 cut(s) 113, 135, 141, 303
BstAFI CTTAAG 1 cut(s) 707
BstBAI YACGTR 1 cut(s) 1061
BstC8I GCNNGC 4 cut(s) 345, 785, 1284, 1413
BstDEI CTNAG 5 cut(s) 171, 333, 735, 755, 1099
BstDSI CCRYGG 2 cut(s) 527, 1028
BstEII GGTNACC 1 cut(s) 560
BstF5I GGATG 4 cut(s) 454, 537, 1039, 1219
BstFNI CGCG 1 cut(s) 468
BstHHI GCGC 1 cut(s) 470
BstKTI GATC 5 cut(s) 352, 595, 625, 898, 1423
BstMAI GTCTC 2 cut(s) 110, 312
BstMBI GATC 5 cut(s) 349, 592, 622, 895, 1420
BstMWI GCNNNNNNNGC 2 cut(s) 269, 1175
BstNI CCWGG 1 cut(s) 722
BstNSI RCATGY 3 cut(s) 787, 847, 991
BstPI GGTNACC 1 cut(s) 560
BstSCI CCNGG 1 cut(s) 720
BstSFI CTRYAG 1 cut(s) 617
BstSLI GKGCMC 2 cut(s) 754, 805
BstUI CGCG 1 cut(s) 468
BstV1I GCAGC 5 cut(s) 10, 281, 1190, 1193, 1273
BstV2I GAAGAC 2 cut(s) 327, 1317
BstX2I RGATCY 3 cut(s) 349, 622, 1420
BstYI RGATCY 3 cut(s) 349, 622, 1420
Bsu36I CCTNAGG 1 cut(s) 171
BsuI GTATCC 2 cut(s) 101, 1144
BsuRI GGCC 2 cut(s) 284, 417
BtgI CCRYGG 2 cut(s) 527, 1028
BtsCI GGATG 4 cut(s) 454, 537, 1039, 1219
BtsI GCAGTG 1 cut(s) 151
BtsIMutI CAGTG 3 cut(s) 151, 752, 1415
Cac8I GCNNGC 4 cut(s) 345, 785, 1284, 1413
CaiI CAGNNNCTG 2 cut(s) 353, 1417
CciI TCATGA 1 cut(s) 10
CfoI GCGC 1 cut(s) 470
Cfr10I RCCGGY 1 cut(s) 553
Cfr13I GGNCC 1 cut(s) 416
CseI GACGC 2 cut(s) 1349, 1360
Csp6I GTAC 3 cut(s) 98, 551, 701
CspAI ACCGGT 1 cut(s) 553
CviQI GTAC 3 cut(s) 98, 551, 701
DdeI CTNAG 5 cut(s) 171, 333, 735, 755, 1099
DpnI GATC 5 cut(s) 351, 594, 624, 897, 1422
DpnII GATC 5 cut(s) 349, 592, 622, 895, 1420
DraI TTTAAA 1 cut(s) 409
DrdI GACNNNNNNGTC 1 cut(s) 1169
DseDI GACNNNNNNGTC 1 cut(s) 1169
Eam1104I CTCTTC 4 cut(s) 113, 135, 141, 303
EarI CTCTTC 4 cut(s) 113, 135, 141, 303
Ecl136II GAGCTC 1 cut(s) 665
Eco130I CCWWGG 4 cut(s) 444, 527, 1028, 1305
Eco147I AGGCCT 1 cut(s) 284
Eco24I GRGCYC 1 cut(s) 667
Eco32I GATATC 1 cut(s) 577
Eco53kI GAGCTC 1 cut(s) 665
Eco57I CTGAAG 2 cut(s) 287, 339
Eco81I CCTNAGG 1 cut(s) 171
Eco88I CYCGRG 2 cut(s) 80, 312
Eco91I GGTNACC 1 cut(s) 560
EcoICRI GAGCTC 1 cut(s) 665
EcoO65I GGTNACC 1 cut(s) 560
EcoRII CCWGG 1 cut(s) 720
EcoRV GATATC 1 cut(s) 577
EcoT14I CCWWGG 4 cut(s) 444, 527, 1028, 1305
EcoT22I ATGCAT 1 cut(s) 779
EcoT38I GRGCYC 1 cut(s) 667
ErhI CCWWGG 4 cut(s) 444, 527, 1028, 1305
Fnu4HI GCNGC 6 cut(s) 24, 270, 466, 1179, 1182, 1287
FokI GGATG 4 cut(s) 461, 544, 1046, 1226
FriOI GRGCYC 1 cut(s) 667
Fsp4HI GCNGC 6 cut(s) 24, 270, 466, 1179, 1182, 1287
FspBI CTAG 6 cut(s) 95, 471, 494, 1140, 1325, 1347
GlaI GCGC 1 cut(s) 469
GluI GCNGC 6 cut(s) 24, 270, 466, 1179, 1182, 1287
HaeIII GGCC 2 cut(s) 284, 417
HapII CCGG 2 cut(s) 554, 1384
HgaI GACGC 2 cut(s) 1349, 1360
HhaI GCGC 1 cut(s) 470
Hin6I GCGC 1 cut(s) 468
HinP1I GCGC 1 cut(s) 468
HincII GTYRAC 3 cut(s) 55, 507, 1162
HindII GTYRAC 3 cut(s) 55, 507, 1162
HpaI GTTAAC 2 cut(s) 55, 507
HpaII CCGG 2 cut(s) 554, 1384
HphI GGTGA 6 cut(s) 134, 186, 208, 487, 554, 956
Hpy166II GTNNAC 6 cut(s) 55, 507, 701, 803, 974, 1162
Hpy188I TCNGA 5 cut(s) 334, 613, 736, 1207, 1448
Hpy8I GTNNAC 6 cut(s) 55, 507, 701, 803, 974, 1162
HpyAV CCTTC 6 cut(s) 61, 156, 407, 868, 934, 1087
HpyCH4IV ACGT 1 cut(s) 1060
HpyF10VI GCNNNNNNNGC 2 cut(s) 269, 1175
HpyF3I CTNAG 5 cut(s) 171, 333, 735, 755, 1099
HpySE526I ACGT 1 cut(s) 1060
HspAI GCGC 1 cut(s) 468
KspAI GTTAAC 2 cut(s) 55, 507
Kzo9I GATC 5 cut(s) 349, 592, 622, 895, 1420
LmnI GCTCC 5 cut(s) 20, 340, 528, 604, 1166
Lsp1109I GCAGC 5 cut(s) 10, 281, 1190, 1193, 1273
LweI GCATC 4 cut(s) 52, 764, 984, 1269
MaeI CTAG 6 cut(s) 95, 471, 494, 1140, 1325, 1347
MaeII ACGT 1 cut(s) 1060
MaeIII GTNAC 2 cut(s) 75, 560
MalI GATC 5 cut(s) 351, 594, 624, 897, 1422
MboI GATC 5 cut(s) 349, 592, 622, 895, 1420
MflI RGATCY 3 cut(s) 349, 622, 1420
MhlI GDGCHC 3 cut(s) 667, 754, 805
MlyI GAGTC 3 cut(s) 83, 317, 809
MmeI TCCRAC 1 cut(s) 318
Mph1103I ATGCAT 1 cut(s) 779
MroXI GAANNNNTTC 2 cut(s) 459, 1391
MseI TTAA 9 cut(s) 54, 62, 258, 408, 506, 708, 714, 891, 936
MslI CAYNNNNRTG 4 cut(s) 82, 696, 782, 992
MspCI CTTAAG 1 cut(s) 707
MspI CCGG 2 cut(s) 554, 1384
MspR9I CCNGG 1 cut(s) 722
Mva1269I GAATGC 4 cut(s) 240, 246, 691, 1274
MvaI CCWGG 1 cut(s) 722
MvnI CGCG 1 cut(s) 468
MwoI GCNNNNNNNGC 2 cut(s) 269, 1175
NcoI CCATGG 2 cut(s) 527, 1028
NdeII GATC 5 cut(s) 349, 592, 622, 895, 1420
NlaIV GGNNCC 2 cut(s) 342, 624
NmeAIII GCCGAG 1 cut(s) 686
NmuCI GTSAC 2 cut(s) 75, 560
NsiI ATGCAT 1 cut(s) 779
NspI RCATGY 3 cut(s) 787, 847, 991
OliI CACNNNNGTG 1 cut(s) 82
PaeI GCATGC 1 cut(s) 787
PaeR7I CTCGAG 1 cut(s) 80
PagI TCATGA 1 cut(s) 10
PceI AGGCCT 1 cut(s) 284
PciI ACATGT 1 cut(s) 987
PcsI WCGNNNNNNNCGW 2 cut(s) 78, 724
PctI GAATGC 4 cut(s) 240, 246, 691, 1274
PdmI GAANNNNTTC 2 cut(s) 459, 1391
PfeI GAWTC 7 cut(s) 7, 207, 731, 741, 1024, 1073, 1328
PinAI ACCGGT 1 cut(s) 553
PkrI GCNGC 6 cut(s) 25, 271, 467, 1180, 1183, 1288
PleI GAGTC 3 cut(s) 82, 317, 809
PpsI GAGTC 3 cut(s) 82, 317, 809
Ppu21I YACGTR 1 cut(s) 1061
PscI ACATGT 1 cut(s) 987
Psp124BI GAGCTC 1 cut(s) 667
Psp6I CCWGG 1 cut(s) 720
PspEI GGTNACC 1 cut(s) 560
PspGI CCWGG 1 cut(s) 720
PspN4I GGNNCC 2 cut(s) 342, 624
PspPI GGNCC 1 cut(s) 416
PspXI VCTCGAGB 1 cut(s) 80
PsrI GAACNNNNNNTAC 2 cut(s) 1180, 1212
PstNI CAGNNNCTG 2 cut(s) 353, 1417
PsuI RGATCY 3 cut(s) 349, 622, 1420
RsaI GTAC 3 cut(s) 99, 552, 702
RsaNI GTAC 3 cut(s) 98, 551, 701
RseI CAYNNNNRTG 4 cut(s) 82, 696, 782, 992
SacI GAGCTC 1 cut(s) 667
SaqAI TTAA 9 cut(s) 54, 62, 258, 408, 506, 708, 714, 891, 936
SatI GCNGC 6 cut(s) 24, 270, 466, 1179, 1182, 1287
Sau3AI GATC 5 cut(s) 349, 592, 622, 895, 1420
Sau96I GGNCC 1 cut(s) 416
ScaI AGTACT 1 cut(s) 99
SchI GAGTC 3 cut(s) 83, 317, 809
ScrFI CCNGG 1 cut(s) 722
SduI GDGCHC 3 cut(s) 667, 754, 805
SfaNI GCATC 4 cut(s) 52, 764, 984, 1269
SfcI CTRYAG 1 cut(s) 617
Sfr274I CTCGAG 1 cut(s) 80
SlaI CTCGAG 1 cut(s) 80
SmiMI CAYNNNNRTG 4 cut(s) 82, 696, 782, 992
SmlI CTYRAG 2 cut(s) 80, 707
SmoI CTYRAG 2 cut(s) 80, 707
SphI GCATGC 1 cut(s) 787
SseBI AGGCCT 1 cut(s) 284
SsiI CCGC 2 cut(s) 466, 886
SspI AATATT 2 cut(s) 570, 1440
SspMI CTAG 6 cut(s) 95, 471, 494, 1140, 1325, 1347
SstI GAGCTC 1 cut(s) 667
StuI AGGCCT 1 cut(s) 284
StyD4I CCNGG 1 cut(s) 720
StyI CCWWGG 4 cut(s) 444, 527, 1028, 1305
TaiI ACGT 1 cut(s) 1063
TaqI TCGA 3 cut(s) 72, 81, 1398
TatI WGTACW 1 cut(s) 97
TauI GCSGC 1 cut(s) 468
TfiI GAWTC 7 cut(s) 7, 207, 731, 741, 1024, 1073, 1328
Tru1I TTAA 9 cut(s) 54, 62, 258, 408, 506, 708, 714, 891, 936
Tru9I TTAA 9 cut(s) 54, 62, 258, 408, 506, 708, 714, 891, 936
TscAI CASTG 3 cut(s) 158, 759, 1422
TseFI GTSAC 2 cut(s) 75, 560
TseI GCWGC 5 cut(s) 23, 269, 1178, 1181, 1286
Tsp45I GTSAC 2 cut(s) 75, 560
TspDTI ATGAA 5 cut(s) 128, 733, 1005, 1055, 1384
TspGWI ACGGA 3 cut(s) 33, 946, 1179
TspRI CASTG 3 cut(s) 158, 759, 1422
Vha464I CTTAAG 1 cut(s) 707
VneI GTGCAC 1 cut(s) 801
XapI RAATTY 2 cut(s) 404, 671
XceI RCATGY 3 cut(s) 787, 847, 991
XhoI CTCGAG 1 cut(s) 80
XmnI GAANNNNTTC 2 cut(s) 459, 1391
XspI CTAG 6 cut(s) 95, 471, 494, 1140, 1325, 1347
ZrmI AGTACT 1 cut(s) 99
Zsp2I ATGCAT 1 cut(s) 779
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.