RchiOBHm_Chr5g0021381

Ribulose-1,5 bisphosphate carboxylase oxygenase large subunit N-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0011165
Physical Location & Seq
Reverse (-)
15596762 .. 15601428
4667 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ30144

Sequence Viewer

Length: 1467 bp
ATGACTTCTTCTCTTCCTCTTCATCACCCTTCCCACTGCCTCATCTCCAATCCTCAGTTGCAGGGCTTCAGCTATGGAGGCTCACCAAGACCCAGATTCTCTTTCACCACTAAAGACACAGACAAAGGCATTCGTATTCAACCCATTAAAGCCAAAGCAGCCACCACCGAGGCCTCTCCGTTTCCTCTGCTTCAGTCTCCCCGAGCTGAAGACTCTCCCTCTGAGTTGGAGCCAACAGATCCTGATTTCTACAAGATAGGGTTTGTTAGAAGTATGAGAGCTTATGGAATTGAATTTAAAGAAGGCCCAGATGGGTTTGGAGTTTATGCTTCCAAGGATGTTGAACCACTTCGCCGTGCTAGGGTGATAATGGAAATACCACTAGAACTGATGTTAACTATAAGCCAAAAGCTCCCATGGATGTTTTTCCCAGATATAGTACCAGTGGGTCACCCAATATTTGATATCATCAATTCAACTGATCCAGAGACAGGTTGGGATCTAAGGTTAGCTTGCCTTCTTTTGTATGCATTTGATCGGGAGGATAACTTTTGGCAGTTGTATAGTGATTTTTTACCCAGTGCAGATGAGTGCCCCAGTCTGCTTTTAGCTAGCGAGGAGGAGCTTTCAGAACTACAGGATCCCAATCTTGCTTCAACAATGAGAGAACAGCAATGCCGAGCTCTGGAATTTTGGGAGAAAAATTGGCATTCTGGTGTACCCCTTAAGATTAAACGCCTCGCTCGAGATTCTGAGAGATTCATCTGGGCACTGAGTATGGCACAATCACGATGCATTGGCATGCAAATTAGAATTGGTGCACTTTTTCAAGACTCAAATATGCTAATTCCTTATGCAGACATGCTAAACCATTCCTTCCAGCCAAATTGTTTTCTCCATTGGCGGTTTAAGGATCGTATGCTTGAAGTAATGATAAATGCTGGACAACGGATTAAGAAAGGTGATGAGATGACTATCAATTACATGAGTGGACAACAGAACAACATGTTGATGCAAAGATATGGCTTTTCTTCATCTGTGAATCCATGGGATGTAATTCAATTCTCTGGAAATGCACGTATTCATTTGGATTCCTTCTTGTCAGTATTCAATATCTCAGGACTTCCCCAAGAATACTATCATAACAATCGCCTATCTAGTGGAGGGGATACTTTTGTTGACGGAGCTGTCATCGCAGCAGCAAGAACATTGCCTACTTGGTCAGATGGGGATGTGCCTCCAATCCCAAGCATGGAAAGACGAGCGATAAAGGAATTACAGGAAGAATGCAGACAGATGCTTGTCAAATTTCCTACCAACTCTAAGGAAGACCAAAATATCCTAGATTCTATGCCAGACGCTACGAGGACGCTTGAAGCTGTGGTCAAGTATAGGTTGCACCGGAAGTTGTTCATCGAGAAAGTCATGCAGACACTGGATCTCTATCAAGAACGAATATTATTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rubis-subs-bind SET

Protein Analysis

488

Amino Acids

56.02

Weight (kDa)

5.59

Isoelectric Point (pI)

52.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SET PF00856 105 - 328 3.3e-10 SET domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011165)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G20130 AT4G20130 AT4G20130
fragaria_vesca FvH4_3g13130 FvH4_3g13130 FvH4_3g13130 FvH4_3g13130 FvH4_3g13130
malus_domestica MD10G1226200.v1.1
prunus_persica Prupe.4G119100_v2.0.a1 Prupe.4G119100_v2.0.a1
pyrus_communis pycom10g19010
rosa_chinensis RchiOBHm_Chr5g0021381
rosa_laevigata RLG00000032608
rosa_multiflora Rmu_sc0004847.1_g000020
rosa_roxburghii Rroxscaffold_1G00057160
rosa_rugosa Rorug05G0066700 Rorug05G0066800
rosa_samantha Rh5CG169100
rosa_wichuraiana Rw5G013880

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1187
AciI CCGC 1 cut(s) 904
AclWI GGATC 7 cut(s) 233, 476, 507, 635, 648, 921, 1446
AcsI RAATTY 3 cut(s) 293, 689, 1307
AcuI CTGAAG 3 cut(s) 52, 176, 228
AfaI GTAC 2 cut(s) 441, 720
AfiI CCNNNNNNNGG 4 cut(s) 361, 491, 685, 1252
AflII CTTAAG 1 cut(s) 725
AflIII ACRYGT 1 cut(s) 1005
Alw21I GWGCWC 2 cut(s) 685, 823
Alw26I GTCTC 2 cut(s) 201, 482
Alw44I GTGCAC 1 cut(s) 819
AlwI GGATC 7 cut(s) 233, 476, 507, 635, 648, 921, 1446
AlwNI CAGNNNCTG 2 cut(s) 242, 1435
Ama87I CYCGRG 2 cut(s) 201, 744
AoxI GGCC 2 cut(s) 171, 304
ApaLI GTGCAC 1 cut(s) 819
ApeKI GCWGC 3 cut(s) 158, 1196, 1199
ApoI RAATTY 3 cut(s) 293, 689, 1307
Asp700I GAANNNNTTC 2 cut(s) 348, 1409
AspS9I GGNCC 1 cut(s) 305
AsuHPI GGTGA 6 cut(s) 17, 75, 97, 376, 443, 974
AsuNHI GCTAGC 1 cut(s) 611
AvaI CYCGRG 2 cut(s) 201, 744
BaeGI GKGCMC 3 cut(s) 596, 772, 823
BaeI ACNNNNGTAYC 2 cut(s) 1161, 1194
BamHI GGATCC 1 cut(s) 640
BanII GRGCYC 1 cut(s) 685
BbsI GAAGAC 2 cut(s) 216, 1335
Bbv12I GWGCWC 2 cut(s) 685, 823
BbvI GCAGC 3 cut(s) 170, 1208, 1211
BccI CCATC 2 cut(s) 305, 1220
BceAI ACGGC 1 cut(s) 339
BciVI GTATCC 1 cut(s) 1162
BcoDI GTCTC 2 cut(s) 201, 482
BfaI CTAG 5 cut(s) 360, 383, 612, 1158, 1343
BfmI CTRYAG 1 cut(s) 635
BfrI CTTAAG 1 cut(s) 725
BfuI GTATCC 1 cut(s) 1162
BisI GCNGC 3 cut(s) 159, 1197, 1200
BlsI GCNGC 3 cut(s) 160, 1198, 1201
BmeT110I CYCGRG 2 cut(s) 201, 744
BmgT120I GGNCC 1 cut(s) 305
BmiI GGNNCC 2 cut(s) 231, 642
BmrI ACTGGG 2 cut(s) 573, 591
BmsI GCATC 3 cut(s) 782, 1002, 1287
BmtI GCTAGC 1 cut(s) 615
BmuI ACTGGG 2 cut(s) 573, 591
BpiI GAAGAC 2 cut(s) 216, 1335
BsaAI YACGTR 1 cut(s) 1079
BsaBI GATNNNNATC 3 cut(s) 645, 974, 1443
BsaJI CCNNGG 4 cut(s) 168, 333, 416, 1046
BsaWI WCCGGW 1 cut(s) 1401
Bsc4I CCNNNNNNNGG 4 cut(s) 361, 491, 685, 1252
Bse1I ACTGG 4 cut(s) 443, 579, 597, 1440
Bse3DI GCAATG 2 cut(s) 680, 1208
Bse8I GATNNNNATC 3 cut(s) 645, 974, 1443
BseDI CCNNGG 4 cut(s) 168, 333, 416, 1046
BseGI GGATG 4 cut(s) 343, 426, 1057, 1237
BseJI GATNNNNATC 3 cut(s) 645, 974, 1443
BseLI CCNNNNNNNGG 4 cut(s) 361, 491, 685, 1252
BseMI GCAATG 2 cut(s) 680, 1208
BseMII CTCAG 5 cut(s) 68, 213, 744, 764, 1131
BseNI ACTGG 4 cut(s) 443, 579, 597, 1440
BseRI GAGGAG 2 cut(s) 632, 635
BseSI GKGCMC 3 cut(s) 596, 772, 823
BseXI GCAGC 3 cut(s) 170, 1208, 1211
BsgI GTGCAG 1 cut(s) 603
BshFI GGCC 2 cut(s) 173, 306
BsiHKAI GWGCWC 2 cut(s) 685, 823
BsiHKCI CYCGRG 2 cut(s) 201, 744
BsiSI CCGG 1 cut(s) 1402
BslI CCNNNNNNNGG 4 cut(s) 361, 491, 685, 1252
BsmAI GTCTC 2 cut(s) 201, 482
BsmI GAATGC 3 cut(s) 129, 709, 1292
BsnI GGCC 2 cut(s) 173, 306
BsoBI CYCGRG 2 cut(s) 201, 744
Bsp1286I GDGCHC 4 cut(s) 596, 685, 772, 823
Bsp143I GATC 7 cut(s) 238, 481, 499, 535, 640, 913, 1438
Bsp19I CCATGG 2 cut(s) 416, 1046
BspACI CCGC 1 cut(s) 904
BspANI GGCC 2 cut(s) 173, 306
BspCNI CTCAG 5 cut(s) 67, 214, 745, 765, 1130
BspLI GGNNCC 2 cut(s) 231, 642
BspOI GCTAGC 1 cut(s) 615
BspPI GGATC 7 cut(s) 233, 476, 507, 635, 648, 921, 1446
BspTI CTTAAG 1 cut(s) 725
BsrDI GCAATG 2 cut(s) 680, 1208
BsrI ACTGG 4 cut(s) 443, 579, 597, 1440
BssECI CCNNGG 4 cut(s) 168, 333, 416, 1046
BssMI GATC 7 cut(s) 238, 481, 499, 535, 640, 913, 1438
BssT1I CCWWGG 3 cut(s) 333, 416, 1046
Bst6I CTCTTC 2 cut(s) 18, 24
BstAFI CTTAAG 1 cut(s) 725
BstBAI YACGTR 1 cut(s) 1079
BstC8I GCNNGC 3 cut(s) 514, 613, 803
BstDEI CTNAG 7 cut(s) 54, 222, 503, 753, 773, 1117, 1323
BstDSI CCRYGG 2 cut(s) 416, 1046
BstEII GGTNACC 1 cut(s) 449
BstF5I GGATG 4 cut(s) 343, 426, 1057, 1237
BstKTI GATC 7 cut(s) 241, 484, 502, 538, 643, 916, 1441
BstMAI GTCTC 2 cut(s) 201, 482
BstMBI GATC 7 cut(s) 238, 481, 499, 535, 640, 913, 1438
BstMWI GCNNNNNNNGC 3 cut(s) 78, 158, 1193
BstNSI RCATGY 3 cut(s) 805, 865, 1009
BstPI GGTNACC 1 cut(s) 449
BstSFI CTRYAG 1 cut(s) 635
BstSLI GKGCMC 3 cut(s) 596, 772, 823
BstV1I GCAGC 3 cut(s) 170, 1208, 1211
BstV2I GAAGAC 2 cut(s) 216, 1335
BstX2I RGATCY 4 cut(s) 238, 499, 640, 1438
BstYI RGATCY 4 cut(s) 238, 499, 640, 1438
BsuI GTATCC 1 cut(s) 1162
BsuRI GGCC 2 cut(s) 173, 306
BtgI CCRYGG 2 cut(s) 416, 1046
BtgZI GCGATG 1 cut(s) 1177
BtsCI GGATG 4 cut(s) 343, 426, 1057, 1237
BtsI GCAGTG 1 cut(s) 34
BtsIMutI CAGTG 5 cut(s) 34, 450, 586, 770, 1433
Cac8I GCNNGC 3 cut(s) 514, 613, 803
CaiI CAGNNNCTG 2 cut(s) 242, 1435
Cfr13I GGNCC 1 cut(s) 305
CseI GACGC 2 cut(s) 1367, 1378
Csp6I GTAC 2 cut(s) 440, 719
CviAII CATG 8 cut(s) 417, 802, 862, 985, 1006, 1047, 1252, 1426
CviQI GTAC 2 cut(s) 440, 719
DdeI CTNAG 7 cut(s) 54, 222, 503, 753, 773, 1117, 1323
DpnI GATC 7 cut(s) 240, 483, 501, 537, 642, 915, 1440
DpnII GATC 7 cut(s) 238, 481, 499, 535, 640, 913, 1438
DraI TTTAAA 1 cut(s) 298
DrdI GACNNNNNNGTC 1 cut(s) 1187
DseDI GACNNNNNNGTC 1 cut(s) 1187
Eam1104I CTCTTC 2 cut(s) 18, 24
EarI CTCTTC 2 cut(s) 18, 24
Ecl136II GAGCTC 1 cut(s) 683
Eco130I CCWWGG 3 cut(s) 333, 416, 1046
Eco147I AGGCCT 1 cut(s) 173
Eco24I GRGCYC 1 cut(s) 685
Eco32I GATATC 1 cut(s) 466
Eco53kI GAGCTC 1 cut(s) 683
Eco57I CTGAAG 3 cut(s) 52, 176, 228
Eco88I CYCGRG 2 cut(s) 201, 744
Eco91I GGTNACC 1 cut(s) 449
EcoICRI GAGCTC 1 cut(s) 683
EcoO65I GGTNACC 1 cut(s) 449
EcoRV GATATC 1 cut(s) 466
EcoT14I CCWWGG 3 cut(s) 333, 416, 1046
EcoT22I ATGCAT 2 cut(s) 532, 797
EcoT38I GRGCYC 1 cut(s) 685
ErhI CCWWGG 3 cut(s) 333, 416, 1046
FaeI CATG 8 cut(s) 420, 805, 865, 988, 1009, 1050, 1255, 1429
FalI AAGNNNNNCTT 2 cut(s) 496, 528
FatI CATG 8 cut(s) 416, 801, 861, 984, 1005, 1046, 1251, 1425
Fnu4HI GCNGC 3 cut(s) 159, 1197, 1200
FokI GGATG 4 cut(s) 350, 433, 1064, 1244
FriOI GRGCYC 1 cut(s) 685
Fsp4HI GCNGC 3 cut(s) 159, 1197, 1200
FspBI CTAG 5 cut(s) 360, 383, 612, 1158, 1343
GluI GCNGC 3 cut(s) 159, 1197, 1200
HaeIII GGCC 2 cut(s) 173, 306
HapII CCGG 1 cut(s) 1402
HgaI GACGC 2 cut(s) 1367, 1378
Hin1II CATG 8 cut(s) 420, 805, 865, 988, 1009, 1050, 1255, 1429
HincII GTYRAC 2 cut(s) 396, 1180
HindII GTYRAC 2 cut(s) 396, 1180
HinfI GANTC 8 cut(s) 96, 212, 749, 759, 833, 1042, 1091, 1346
HpaI GTTAAC 1 cut(s) 396
HpaII CCGG 1 cut(s) 1402
HphI GGTGA 6 cut(s) 17, 75, 97, 376, 443, 974
Hpy166II GTNNAC 5 cut(s) 396, 719, 821, 992, 1180
Hpy188I TCNGA 5 cut(s) 223, 631, 754, 1225, 1466
Hpy8I GTNNAC 5 cut(s) 396, 719, 821, 992, 1180
HpyAV CCTTC 5 cut(s) 39, 296, 527, 886, 1105
HpyCH4IV ACGT 1 cut(s) 1078
HpyF10VI GCNNNNNNNGC 3 cut(s) 78, 158, 1193
HpyF3I CTNAG 7 cut(s) 54, 222, 503, 753, 773, 1117, 1323
HpySE526I ACGT 1 cut(s) 1078
Hsp92II CATG 8 cut(s) 420, 805, 865, 988, 1009, 1050, 1255, 1429
KspAI GTTAAC 1 cut(s) 396
Kzo9I GATC 7 cut(s) 238, 481, 499, 535, 640, 913, 1438
LmnI GCTCC 4 cut(s) 229, 417, 622, 1184
Lsp1109I GCAGC 3 cut(s) 170, 1208, 1211
LweI GCATC 3 cut(s) 782, 1002, 1287
MaeI CTAG 5 cut(s) 360, 383, 612, 1158, 1343
MaeII ACGT 1 cut(s) 1078
MaeIII GTNAC 1 cut(s) 449
MalI GATC 7 cut(s) 240, 483, 501, 537, 642, 915, 1440
MboI GATC 7 cut(s) 238, 481, 499, 535, 640, 913, 1438
MboII GAAGA 6 cut(s) 5, 11, 221, 1023, 1295, 1340
MflI RGATCY 4 cut(s) 238, 499, 640, 1438
MhlI GDGCHC 4 cut(s) 596, 685, 772, 823
MlyI GAGTC 2 cut(s) 206, 827
MmeI TCCRAC 1 cut(s) 207
Mph1103I ATGCAT 2 cut(s) 532, 797
MroXI GAANNNNTTC 2 cut(s) 348, 1409
MseI TTAA 7 cut(s) 147, 297, 395, 726, 732, 909, 954
MslI CAYNNNNRTG 3 cut(s) 714, 800, 1010
MspCI CTTAAG 1 cut(s) 725
MspI CCGG 1 cut(s) 1402
Mva1269I GAATGC 3 cut(s) 129, 709, 1292
MwoI GCNNNNNNNGC 3 cut(s) 78, 158, 1193
NcoI CCATGG 2 cut(s) 416, 1046
NdeII GATC 7 cut(s) 238, 481, 499, 535, 640, 913, 1438
NheI GCTAGC 1 cut(s) 611
NlaIII CATG 8 cut(s) 420, 805, 865, 988, 1009, 1050, 1255, 1429
NlaIV GGNNCC 2 cut(s) 231, 642
NmeAIII GCCGAG 1 cut(s) 704
NmuCI GTSAC 1 cut(s) 449
NsiI ATGCAT 2 cut(s) 532, 797
NspI RCATGY 3 cut(s) 805, 865, 1009
PaeI GCATGC 1 cut(s) 805
PaeR7I CTCGAG 1 cut(s) 744
PceI AGGCCT 1 cut(s) 173
PciI ACATGT 1 cut(s) 1005
PcsI WCGNNNNNNNCGW 1 cut(s) 742
PctI GAATGC 3 cut(s) 129, 709, 1292
PdmI GAANNNNTTC 2 cut(s) 348, 1409
PfeI GAWTC 6 cut(s) 96, 749, 759, 1042, 1091, 1346
PkrI GCNGC 3 cut(s) 160, 1198, 1201
PleI GAGTC 2 cut(s) 206, 827
PpsI GAGTC 2 cut(s) 206, 827
Ppu21I YACGTR 1 cut(s) 1079
PscI ACATGT 1 cut(s) 1005
Psp124BI GAGCTC 1 cut(s) 685
PspEI GGTNACC 1 cut(s) 449
PspN4I GGNNCC 2 cut(s) 231, 642
PspPI GGNCC 1 cut(s) 305
PsrI GAACNNNNNNTAC 2 cut(s) 1198, 1230
PstNI CAGNNNCTG 2 cut(s) 242, 1435
PsuI RGATCY 4 cut(s) 238, 499, 640, 1438
RsaI GTAC 2 cut(s) 441, 720
RsaNI GTAC 2 cut(s) 440, 719
RseI CAYNNNNRTG 3 cut(s) 714, 800, 1010
SacI GAGCTC 1 cut(s) 685
SaqAI TTAA 7 cut(s) 147, 297, 395, 726, 732, 909, 954
SatI GCNGC 3 cut(s) 159, 1197, 1200
Sau3AI GATC 7 cut(s) 238, 481, 499, 535, 640, 913, 1438
Sau96I GGNCC 1 cut(s) 305
SchI GAGTC 2 cut(s) 206, 827
SduI GDGCHC 4 cut(s) 596, 685, 772, 823
SfaNI GCATC 3 cut(s) 782, 1002, 1287
SfcI CTRYAG 1 cut(s) 635
Sfr274I CTCGAG 1 cut(s) 744
SlaI CTCGAG 1 cut(s) 744
SmiMI CAYNNNNRTG 3 cut(s) 714, 800, 1010
SmlI CTYRAG 2 cut(s) 725, 744
SmoI CTYRAG 2 cut(s) 725, 744
SphI GCATGC 1 cut(s) 805
SseBI AGGCCT 1 cut(s) 173
SsiI CCGC 1 cut(s) 904
SspI AATATT 2 cut(s) 459, 1458
SspMI CTAG 5 cut(s) 360, 383, 612, 1158, 1343
SstI GAGCTC 1 cut(s) 685
StuI AGGCCT 1 cut(s) 173
StyI CCWWGG 3 cut(s) 333, 416, 1046
TaiI ACGT 1 cut(s) 1081
TaqI TCGA 2 cut(s) 745, 1416
TfiI GAWTC 6 cut(s) 96, 749, 759, 1042, 1091, 1346
Tru1I TTAA 7 cut(s) 147, 297, 395, 726, 732, 909, 954
Tru9I TTAA 7 cut(s) 147, 297, 395, 726, 732, 909, 954
TscAI CASTG 5 cut(s) 41, 450, 586, 777, 1440
TseFI GTSAC 1 cut(s) 449
TseI GCWGC 3 cut(s) 158, 1196, 1199
Tsp45I GTSAC 1 cut(s) 449
TspDTI ATGAA 5 cut(s) 11, 751, 1023, 1073, 1402
TspGWI ACGGA 3 cut(s) 168, 964, 1197
TspRI CASTG 5 cut(s) 41, 450, 586, 777, 1440
Vha464I CTTAAG 1 cut(s) 725
VneI GTGCAC 1 cut(s) 819
XapI RAATTY 3 cut(s) 293, 689, 1307
XceI RCATGY 3 cut(s) 805, 865, 1009
XcmI CCANNNNNNNNNTGG 1 cut(s) 492
XhoI CTCGAG 1 cut(s) 744
XmnI GAANNNNTTC 2 cut(s) 348, 1409
XspI CTAG 5 cut(s) 360, 383, 612, 1158, 1343
Zsp2I ATGCAT 2 cut(s) 532, 797
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.