Rw5G013880

Ribulose-1,5 bisphosphate carboxylase oxygenase large subunit N-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0011165
Physical Location & Seq
Reverse (-)
16387240 .. 16391495
4256 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G013880.1

Sequence Viewer

Length: 1470 bp
ATGGCTTCTTCTCTTCCTCTTCATCACCCTTCCCACTGCCTCATCTCCAATCCTCAGTTGCAGGGCTTCAACTATGGAGGCTCACCAAGACCCAGATTCGCTTTCACCACTAAAGACACAGACAAAGGCATTCGCATTCAACCCATTAAAGCCAAAGCAGCCACCACCGAGGCCTCTCCGTTTCCTCTGCTTCAGTCTCCCCGAGCTGAAGACTCTCCCTCTGAGTTAGAGCCAGCAGATCCGGATTTCTACAAGATAGGGTTTGTTAGAAGTATGAGAGCTTATGGAATTGAATTTAAAGAAGGCCCAGATGGGTTTGGAGTTTATGCTTCCAAGGATGTTGAACCACTTCGCCGTGCTAGGGTGATAATGGAAATACCACTAGAACTGATGTTAACTATAAGCCAACAGCTCCCATGGATGTTTTTCCCAGATATAGTACCGGTGGGTCACCCAATATTTGATATCATCAATTCAACTGATCCAGAGACAGGTTGGGATCTAAGGTTAGCTTGCCTTCTTTTGTATGCATTTGATCGGGAGGATAACTTTTGGCAGTTGTATGGTGATTTTTTACCCAGTGCAGATGAGTGCCCCAGTCTGCTTTTAGCTAGCGAGGAGGAGCTTTCAGAACTACAGGATCCCAATCTTGCTTCAACAATGAGAGAACAGCAACGCCGAGCTCTGGAATTTTGGGAGAAAAATTGGCATTCTGGTGTACCCCTTAAGATTAAACGCCTCGCTCGTGATTCTGAGAGATTCATTTGGGCACTGAGTATGGCACAATCACGATGCATTGGCATGCAAATTAGAATTGGTGCACTTTTTCAAGACTCAAATATGCTAATTCCTTATGCAGACATGCTAAACCATTCCTTCCAGCCAAATTGTTTTCTCCATTGGCGGTTTAAGGATCGTATGCTTGAAGTAATGATAAATGCTGGACAACGGATTAAGAAAGGTGATGAGATGACTATCAATTACATGAGTGGACAACAGAACAACATGTTGATGCAAAGATATGGCTTTTCTTCATCTGTGAATCCATGGGATGTAATTCAATTCTCTGGAAATGCACGTATTCATTTGGATTCCTTCTTGTCAGTATTCAATATCTCAGGACTTCCCCAAGAATACTATCATAACACAGATCGCCTATCTAGTGGAGGGGATACTTTTGTTGACGGAGCTGTCATTGCAGCAGCAAGAACATTGCCTACTTGGTCAGATGGGGATGTGCCTCCAATCCCAAGCATGGAAAGACGAGCGATAAAGGAATTACAGGAAGAATGCAGGCAGATGCTTGCCGCATTTCCTACCAACTCTAAGGAAGACCAAAATATCCTAGATTCTATGCCAGACGCTGCTAGGACGCTTGAAGCTGTGGTCAAGTATAGGTTGCACCGGAAGTTGTTCATCGAGAAAGTCATGCAGGCACTGGATCTATATCAAGAACGAATATTATTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rubis-subs-bind SET

Protein Analysis

489

Amino Acids

55.95

Weight (kDa)

5.43

Isoelectric Point (pI)

53.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SET PF00856 105 - 328 3.8e-10 SET domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011165)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G20130 AT4G20130 AT4G20130
fragaria_vesca FvH4_3g13130 FvH4_3g13130 FvH4_3g13130 FvH4_3g13130 FvH4_3g13130
malus_domestica MD10G1226200.v1.1
prunus_persica Prupe.4G119100_v2.0.a1 Prupe.4G119100_v2.0.a1
pyrus_communis pycom10g19010
rosa_chinensis RchiOBHm_Chr5g0021381
rosa_laevigata RLG00000032608
rosa_multiflora Rmu_sc0004847.1_g000020
rosa_roxburghii Rroxscaffold_1G00057160
rosa_rugosa Rorug05G0066700 Rorug05G0066800
rosa_samantha Rh5CG169100
rosa_wichuraiana Rw5G013880

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1190
AccIII TCCGGA 1 cut(s) 241
AciI CCGC 2 cut(s) 904, 1308
AclWI GGATC 7 cut(s) 233, 476, 507, 635, 648, 921, 1449
AcsI RAATTY 2 cut(s) 293, 689
AcuI CTGAAG 2 cut(s) 176, 228
AfaI GTAC 2 cut(s) 441, 720
AfiI CCNNNNNNNGG 4 cut(s) 361, 491, 685, 1255
AflII CTTAAG 1 cut(s) 725
AflIII ACRYGT 1 cut(s) 1005
AgeI ACCGGT 1 cut(s) 442
AluBI AGCT 9 cut(s) 206, 281, 412, 512, 611, 625, 683, 1190, 1382
AluI AGCT 9 cut(s) 206, 281, 412, 512, 611, 625, 683, 1190, 1382
Alw21I GWGCWC 2 cut(s) 685, 823
Alw26I GTCTC 2 cut(s) 201, 482
Alw44I GTGCAC 1 cut(s) 819
AlwI GGATC 7 cut(s) 233, 476, 507, 635, 648, 921, 1449
AlwNI CAGNNNCTG 2 cut(s) 1364, 1438
Ama87I CYCGRG 1 cut(s) 201
Aor13HI TCCGGA 1 cut(s) 241
AoxI GGCC 2 cut(s) 171, 304
ApaLI GTGCAC 1 cut(s) 819
ApeKI GCWGC 4 cut(s) 158, 1199, 1202, 1364
ApoI RAATTY 2 cut(s) 293, 689
AsiGI ACCGGT 1 cut(s) 442
Asp700I GAANNNNTTC 2 cut(s) 348, 1412
AspS9I GGNCC 1 cut(s) 305
AsuHPI GGTGA 7 cut(s) 17, 75, 97, 376, 443, 578, 974
AsuNHI GCTAGC 1 cut(s) 611
AvaI CYCGRG 1 cut(s) 201
BaeGI GKGCMC 3 cut(s) 596, 772, 823
BaeI ACNNNNGTAYC 2 cut(s) 1164, 1197
BamHI GGATCC 1 cut(s) 640
BanII GRGCYC 1 cut(s) 685
BauI CACGAG 1 cut(s) 744
BbsI GAAGAC 2 cut(s) 216, 1338
Bbv12I GWGCWC 2 cut(s) 685, 823
BbvI GCAGC 4 cut(s) 170, 1211, 1214, 1351
BccI CCATC 2 cut(s) 305, 1223
BceAI ACGGC 1 cut(s) 339
BciVI GTATCC 1 cut(s) 1165
BcoDI GTCTC 2 cut(s) 201, 482
BfaI CTAG 6 cut(s) 360, 383, 612, 1161, 1346, 1368
BfmI CTRYAG 1 cut(s) 635
BfrI CTTAAG 1 cut(s) 725
BfuI GTATCC 1 cut(s) 1165
BisI GCNGC 5 cut(s) 159, 1200, 1203, 1308, 1365
BlsI GCNGC 5 cut(s) 160, 1201, 1204, 1309, 1366
BmeT110I CYCGRG 1 cut(s) 201
BmgT120I GGNCC 1 cut(s) 305
BmiI GGNNCC 1 cut(s) 642
BmrI ACTGGG 2 cut(s) 573, 591
BmsI GCATC 3 cut(s) 782, 1002, 1290
BmtI GCTAGC 1 cut(s) 615
BmuI ACTGGG 2 cut(s) 573, 591
BpiI GAAGAC 2 cut(s) 216, 1338
BsaAI YACGTR 1 cut(s) 1079
BsaBI GATNNNNATC 3 cut(s) 645, 974, 1446
BsaJI CCNNGG 4 cut(s) 168, 333, 416, 1046
BsaWI WCCGGW 3 cut(s) 241, 442, 1404
Bsc4I CCNNNNNNNGG 4 cut(s) 361, 491, 685, 1255
Bse118I RCCGGY 1 cut(s) 442
Bse1I ACTGG 3 cut(s) 579, 597, 1443
Bse3DI GCAATG 2 cut(s) 1194, 1211
Bse8I GATNNNNATC 3 cut(s) 645, 974, 1446
BseAI TCCGGA 1 cut(s) 241
BseDI CCNNGG 4 cut(s) 168, 333, 416, 1046
BseGI GGATG 4 cut(s) 343, 426, 1057, 1240
BseJI GATNNNNATC 3 cut(s) 645, 974, 1446
BseLI CCNNNNNNNGG 4 cut(s) 361, 491, 685, 1255
BseMI GCAATG 2 cut(s) 1194, 1211
BseMII CTCAG 5 cut(s) 68, 213, 744, 764, 1131
BseNI ACTGG 3 cut(s) 579, 597, 1443
BseRI GAGGAG 2 cut(s) 632, 635
BseSI GKGCMC 3 cut(s) 596, 772, 823
BseXI GCAGC 4 cut(s) 170, 1211, 1214, 1351
BsgI GTGCAG 1 cut(s) 603
BshFI GGCC 2 cut(s) 173, 306
BshTI ACCGGT 1 cut(s) 442
BsiHKAI GWGCWC 2 cut(s) 685, 823
BsiHKCI CYCGRG 1 cut(s) 201
BsiSI CCGG 3 cut(s) 242, 443, 1405
BslI CCNNNNNNNGG 4 cut(s) 361, 491, 685, 1255
BsmAI GTCTC 2 cut(s) 201, 482
BsmI GAATGC 4 cut(s) 129, 135, 709, 1295
BsnI GGCC 2 cut(s) 173, 306
BsoBI CYCGRG 1 cut(s) 201
Bsp1286I GDGCHC 4 cut(s) 596, 685, 772, 823
Bsp13I TCCGGA 1 cut(s) 241
Bsp143I GATC 8 cut(s) 238, 481, 499, 535, 640, 913, 1150, 1441
Bsp19I CCATGG 2 cut(s) 416, 1046
BspACI CCGC 2 cut(s) 904, 1308
BspANI GGCC 2 cut(s) 173, 306
BspCNI CTCAG 5 cut(s) 67, 214, 745, 765, 1130
BspEI TCCGGA 1 cut(s) 241
BspLI GGNNCC 1 cut(s) 642
BspOI GCTAGC 1 cut(s) 615
BspPI GGATC 7 cut(s) 233, 476, 507, 635, 648, 921, 1449
BspTI CTTAAG 1 cut(s) 725
BsrDI GCAATG 2 cut(s) 1194, 1211
BsrFI RCCGGY 1 cut(s) 442
BsrI ACTGG 3 cut(s) 579, 597, 1443
BssAI RCCGGY 1 cut(s) 442
BssECI CCNNGG 4 cut(s) 168, 333, 416, 1046
BssMI GATC 8 cut(s) 238, 481, 499, 535, 640, 913, 1150, 1441
BssSI CACGAG 1 cut(s) 744
BssT1I CCWWGG 3 cut(s) 333, 416, 1046
Bst2BI CACGAG 1 cut(s) 744
Bst6I CTCTTC 2 cut(s) 18, 24
BstAFI CTTAAG 1 cut(s) 725
BstBAI YACGTR 1 cut(s) 1079
BstC8I GCNNGC 7 cut(s) 234, 514, 613, 803, 1295, 1305, 1434
BstDEI CTNAG 7 cut(s) 54, 222, 503, 753, 773, 1117, 1326
BstDSI CCRYGG 2 cut(s) 416, 1046
BstEII GGTNACC 1 cut(s) 449
BstF5I GGATG 4 cut(s) 343, 426, 1057, 1240
BstKTI GATC 8 cut(s) 241, 484, 502, 538, 643, 916, 1153, 1444
BstMAI GTCTC 2 cut(s) 201, 482
BstMBI GATC 8 cut(s) 238, 481, 499, 535, 640, 913, 1150, 1441
BstMWI GCNNNNNNNGC 2 cut(s) 158, 1196
BstNSI RCATGY 3 cut(s) 805, 865, 1009
BstPI GGTNACC 1 cut(s) 449
BstSFI CTRYAG 1 cut(s) 635
BstSLI GKGCMC 3 cut(s) 596, 772, 823
BstV1I GCAGC 4 cut(s) 170, 1211, 1214, 1351
BstV2I GAAGAC 2 cut(s) 216, 1338
BstX2I RGATCY 4 cut(s) 238, 499, 640, 1441
BstYI RGATCY 4 cut(s) 238, 499, 640, 1441
BsuI GTATCC 1 cut(s) 1165
BsuRI GGCC 2 cut(s) 173, 306
BtgI CCRYGG 2 cut(s) 416, 1046
BtsCI GGATG 4 cut(s) 343, 426, 1057, 1240
BtsI GCAGTG 1 cut(s) 34
BtsIMutI CAGTG 4 cut(s) 34, 586, 770, 1436
Cac8I GCNNGC 7 cut(s) 234, 514, 613, 803, 1295, 1305, 1434
CaiI CAGNNNCTG 2 cut(s) 1364, 1438
Cfr10I RCCGGY 1 cut(s) 442
Cfr13I GGNCC 1 cut(s) 305
CseI GACGC 2 cut(s) 1370, 1381
Csp6I GTAC 2 cut(s) 440, 719
CspAI ACCGGT 1 cut(s) 442
CviAII CATG 8 cut(s) 417, 802, 862, 985, 1006, 1047, 1255, 1429
CviQI GTAC 2 cut(s) 440, 719
DdeI CTNAG 7 cut(s) 54, 222, 503, 753, 773, 1117, 1326
DpnI GATC 8 cut(s) 240, 483, 501, 537, 642, 915, 1152, 1443
DpnII GATC 8 cut(s) 238, 481, 499, 535, 640, 913, 1150, 1441
DraI TTTAAA 1 cut(s) 298
DrdI GACNNNNNNGTC 1 cut(s) 1190
DseDI GACNNNNNNGTC 1 cut(s) 1190
Eam1104I CTCTTC 2 cut(s) 18, 24
EarI CTCTTC 2 cut(s) 18, 24
Ecl136II GAGCTC 1 cut(s) 683
Eco130I CCWWGG 3 cut(s) 333, 416, 1046
Eco147I AGGCCT 1 cut(s) 173
Eco24I GRGCYC 1 cut(s) 685
Eco32I GATATC 1 cut(s) 466
Eco53kI GAGCTC 1 cut(s) 683
Eco57I CTGAAG 2 cut(s) 176, 228
Eco88I CYCGRG 1 cut(s) 201
Eco91I GGTNACC 1 cut(s) 449
EcoICRI GAGCTC 1 cut(s) 683
EcoO65I GGTNACC 1 cut(s) 449
EcoRV GATATC 1 cut(s) 466
EcoT14I CCWWGG 3 cut(s) 333, 416, 1046
EcoT22I ATGCAT 2 cut(s) 532, 797
EcoT38I GRGCYC 1 cut(s) 685
ErhI CCWWGG 3 cut(s) 333, 416, 1046
FaeI CATG 8 cut(s) 420, 805, 865, 988, 1009, 1050, 1258, 1432
FalI AAGNNNNNCTT 2 cut(s) 496, 528
FatI CATG 8 cut(s) 416, 801, 861, 984, 1005, 1046, 1254, 1428
Fnu4HI GCNGC 5 cut(s) 159, 1200, 1203, 1308, 1365
FokI GGATG 4 cut(s) 350, 433, 1064, 1247
FriOI GRGCYC 1 cut(s) 685
Fsp4HI GCNGC 5 cut(s) 159, 1200, 1203, 1308, 1365
FspBI CTAG 6 cut(s) 360, 383, 612, 1161, 1346, 1368
GluI GCNGC 5 cut(s) 159, 1200, 1203, 1308, 1365
HaeIII GGCC 2 cut(s) 173, 306
HapII CCGG 3 cut(s) 242, 443, 1405
HgaI GACGC 2 cut(s) 1370, 1381
Hin1II CATG 8 cut(s) 420, 805, 865, 988, 1009, 1050, 1258, 1432
HincII GTYRAC 2 cut(s) 396, 1183
HindII GTYRAC 2 cut(s) 396, 1183
HinfI GANTC 8 cut(s) 96, 212, 749, 759, 833, 1042, 1091, 1349
HpaI GTTAAC 1 cut(s) 396
HpaII CCGG 3 cut(s) 242, 443, 1405
HphI GGTGA 7 cut(s) 17, 75, 97, 376, 443, 578, 974
Hpy166II GTNNAC 5 cut(s) 396, 719, 821, 992, 1183
Hpy188I TCNGA 5 cut(s) 223, 631, 754, 1228, 1469
Hpy8I GTNNAC 5 cut(s) 396, 719, 821, 992, 1183
HpyAV CCTTC 5 cut(s) 39, 296, 527, 886, 1105
HpyCH4IV ACGT 1 cut(s) 1078
HpyF10VI GCNNNNNNNGC 2 cut(s) 158, 1196
HpyF3I CTNAG 7 cut(s) 54, 222, 503, 753, 773, 1117, 1326
HpySE526I ACGT 1 cut(s) 1078
Hsp92II CATG 8 cut(s) 420, 805, 865, 988, 1009, 1050, 1258, 1432
Kpn2I TCCGGA 1 cut(s) 241
KspAI GTTAAC 1 cut(s) 396
Kzo9I GATC 8 cut(s) 238, 481, 499, 535, 640, 913, 1150, 1441
LmnI GCTCC 3 cut(s) 417, 622, 1187
Lsp1109I GCAGC 4 cut(s) 170, 1211, 1214, 1351
LweI GCATC 3 cut(s) 782, 1002, 1290
MaeI CTAG 6 cut(s) 360, 383, 612, 1161, 1346, 1368
MaeII ACGT 1 cut(s) 1078
MaeIII GTNAC 1 cut(s) 449
MalI GATC 8 cut(s) 240, 483, 501, 537, 642, 915, 1152, 1443
MboI GATC 8 cut(s) 238, 481, 499, 535, 640, 913, 1150, 1441
MboII GAAGA 6 cut(s) 5, 11, 221, 1023, 1298, 1343
MflI RGATCY 4 cut(s) 238, 499, 640, 1441
MhlI GDGCHC 4 cut(s) 596, 685, 772, 823
MlyI GAGTC 2 cut(s) 206, 827
Mph1103I ATGCAT 2 cut(s) 532, 797
MroI TCCGGA 1 cut(s) 241
MroXI GAANNNNTTC 2 cut(s) 348, 1412
MseI TTAA 7 cut(s) 147, 297, 395, 726, 732, 909, 954
MslI CAYNNNNRTG 3 cut(s) 714, 800, 1010
MspCI CTTAAG 1 cut(s) 725
MspI CCGG 3 cut(s) 242, 443, 1405
Mva1269I GAATGC 4 cut(s) 129, 135, 709, 1295
MwoI GCNNNNNNNGC 2 cut(s) 158, 1196
NcoI CCATGG 2 cut(s) 416, 1046
NdeII GATC 8 cut(s) 238, 481, 499, 535, 640, 913, 1150, 1441
NheI GCTAGC 1 cut(s) 611
NlaIII CATG 8 cut(s) 420, 805, 865, 988, 1009, 1050, 1258, 1432
NlaIV GGNNCC 1 cut(s) 642
NmeAIII GCCGAG 1 cut(s) 704
NmuCI GTSAC 1 cut(s) 449
NsiI ATGCAT 2 cut(s) 532, 797
NspI RCATGY 3 cut(s) 805, 865, 1009
PaeI GCATGC 1 cut(s) 805
PceI AGGCCT 1 cut(s) 173
PciI ACATGT 1 cut(s) 1005
PcsI WCGNNNNNNNCGW 1 cut(s) 742
PctI GAATGC 4 cut(s) 129, 135, 709, 1295
PdmI GAANNNNTTC 2 cut(s) 348, 1412
PfeI GAWTC 6 cut(s) 96, 749, 759, 1042, 1091, 1349
PinAI ACCGGT 1 cut(s) 442
PkrI GCNGC 5 cut(s) 160, 1201, 1204, 1309, 1366
PleI GAGTC 2 cut(s) 206, 827
PpsI GAGTC 2 cut(s) 206, 827
Ppu21I YACGTR 1 cut(s) 1079
PscI ACATGT 1 cut(s) 1005
Psp124BI GAGCTC 1 cut(s) 685
PspEI GGTNACC 1 cut(s) 449
PspN4I GGNNCC 1 cut(s) 642
PspPI GGNCC 1 cut(s) 305
PsrI GAACNNNNNNTAC 2 cut(s) 1201, 1233
PstNI CAGNNNCTG 2 cut(s) 1364, 1438
PsuI RGATCY 4 cut(s) 238, 499, 640, 1441
RsaI GTAC 2 cut(s) 441, 720
RsaNI GTAC 2 cut(s) 440, 719
RseI CAYNNNNRTG 3 cut(s) 714, 800, 1010
SacI GAGCTC 1 cut(s) 685
SaqAI TTAA 7 cut(s) 147, 297, 395, 726, 732, 909, 954
SatI GCNGC 5 cut(s) 159, 1200, 1203, 1308, 1365
Sau3AI GATC 8 cut(s) 238, 481, 499, 535, 640, 913, 1150, 1441
Sau96I GGNCC 1 cut(s) 305
SchI GAGTC 2 cut(s) 206, 827
SduI GDGCHC 4 cut(s) 596, 685, 772, 823
SfaNI GCATC 3 cut(s) 782, 1002, 1290
SfcI CTRYAG 1 cut(s) 635
SmiMI CAYNNNNRTG 3 cut(s) 714, 800, 1010
SmlI CTYRAG 1 cut(s) 725
SmoI CTYRAG 1 cut(s) 725
SphI GCATGC 1 cut(s) 805
SseBI AGGCCT 1 cut(s) 173
SsiI CCGC 2 cut(s) 904, 1308
SspI AATATT 2 cut(s) 459, 1461
SspMI CTAG 6 cut(s) 360, 383, 612, 1161, 1346, 1368
SstI GAGCTC 1 cut(s) 685
StuI AGGCCT 1 cut(s) 173
StyI CCWWGG 3 cut(s) 333, 416, 1046
TaiI ACGT 1 cut(s) 1081
TaqI TCGA 1 cut(s) 1419
TauI GCSGC 1 cut(s) 1310
TfiI GAWTC 6 cut(s) 96, 749, 759, 1042, 1091, 1349
Tru1I TTAA 7 cut(s) 147, 297, 395, 726, 732, 909, 954
Tru9I TTAA 7 cut(s) 147, 297, 395, 726, 732, 909, 954
TscAI CASTG 4 cut(s) 41, 586, 777, 1443
TseFI GTSAC 1 cut(s) 449
TseI GCWGC 4 cut(s) 158, 1199, 1202, 1364
Tsp45I GTSAC 1 cut(s) 449
TspDTI ATGAA 5 cut(s) 11, 751, 1023, 1073, 1405
TspGWI ACGGA 3 cut(s) 168, 964, 1200
TspRI CASTG 4 cut(s) 41, 586, 777, 1443
Vha464I CTTAAG 1 cut(s) 725
VneI GTGCAC 1 cut(s) 819
XapI RAATTY 2 cut(s) 293, 689
XceI RCATGY 3 cut(s) 805, 865, 1009
XcmI CCANNNNNNNNNTGG 1 cut(s) 492
XmnI GAANNNNTTC 2 cut(s) 348, 1412
XspI CTAG 6 cut(s) 360, 383, 612, 1161, 1346, 1368
Zsp2I ATGCAT 2 cut(s) 532, 797
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.