RLG00000032608

Ribulose-1,5 bisphosphate carboxylase oxygenase large subunit N-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0011165
Physical Location & Seq
Reverse (-)
16945375 .. 16949615
4241 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000032608

Sequence Viewer

Length: 1347 bp
ATGGCTTCTTCTCTTCCTCTTCATCATCCCTCCCACTGCCTCATCTCTAATCCACAGGGCTTCAACTATGGAGGCTCACCAAGACCCAGATTCTCTTTCACCACTAAAGACACAGACAAAGGCATTCGCATTCAACCCATTAAAGCCAAAGCAGCCACCGAGGCCTCTCCATTTCCTTTGCTTCAGTCTCCCCAAGCTGAAGACTCTCCCTCTGAGGATGTTGAACCACTTCGCCGTGCTAGGGTGATAATGGAAATACCACTAGAACTGATGTTAACTATAAGCCAAAAGCTCCCATGGATGTTTTTCCCAGATATAGTACCGGTGGGTCACCCAATATTTGATATCATCAATTCAACTGATCCAGAGACAGGTTGGGATCTAAGGTTAGCTTGCCTTCTTTTGTATGCATTTGATCGGGAGGATAACTTTTGGCAGTTGTATAGTGATTTTTTACCCAGTGCAGATGAGTGCCCCAGTCTGCTTTTAGCTAGCGAGGAGGAGCTTTCAGAACTACAGGATCCCAATCTTGCTTCAACAATGAGAGAACAGCAACGCCGAGCTCTGGAATTTTGGGAGAAAAATTGGCATTCTGGTGTACCCCTTAAGATTAAACGCCTCGCTCGAGATTCTGAGAGATTCATTTGGGCACTGAGTATGGCACAATCACGATGCATTGGCATGCAAATTAGAATTGGTGCACTTTTTCAAGACTCAAATATGCTAATTCCTTATGCAGACATGCTAAACCATTCCTTCCAGCCAAATTGTTTTCTCCATTGGCGGTTTAAGGATCGTATGCTTGAAGTAATGATAAATGCTGGACAACAGATTAAGAAAGGTGATGAGATGACTATCAATTACATGAGTGGACAACAGAACAACATGTTGATGCAAAGATATGGCTTTTCTTCATCTGTGAATCCATGGGATGTAATTCAATTCTCTGGAAATGCACGTATTCATTTGGATTCCTTCTTGTCAGTATTCAATATCTCAGGACTTCCCCAAGAATACTATCATAACAATCGCCTATCTAGTGGAGGGGATACTTTTGTTGACGGAGCTGTCATTGCAGCAGCAAGAACATTGCCTACTTGGTCAGATGGGGATGTGCCTCCAATCCCAAGCATGGAAAGACGAGCGATAAAGGAATTACAGGAAGAATGCAGACAGATGCTTGCCACATTTCCTACCAACTCTAAGGAAGACCAAAATATCCTAGATTCTATGCCAGATGCTACGAGGACGCTTGAAGCTGTGGTCAAGTATAGGTTGCACCGGAAGTTGTTTATCGAGAAAGTCATGCAGACACTGGATCTCTATCAAGAACGAATATTATTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rubis-subs-bind SET

Protein Analysis

449

Amino Acids

51.44

Weight (kDa)

5.48

Isoelectric Point (pI)

56.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SET PF00856 200 - 288 1e-09 SET domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011165)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G20130 AT4G20130 AT4G20130
fragaria_vesca FvH4_3g13130 FvH4_3g13130 FvH4_3g13130 FvH4_3g13130 FvH4_3g13130
malus_domestica MD10G1226200.v1.1
prunus_persica Prupe.4G119100_v2.0.a1 Prupe.4G119100_v2.0.a1
pyrus_communis pycom10g19010
rosa_chinensis RchiOBHm_Chr5g0021381
rosa_laevigata RLG00000032608
rosa_multiflora Rmu_sc0004847.1_g000020
rosa_roxburghii Rroxscaffold_1G00057160
rosa_rugosa Rorug05G0066700 Rorug05G0066800
rosa_samantha Rh5CG169100
rosa_wichuraiana Rw5G013880

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1067
AciI CCGC 1 cut(s) 784
AclWI GGATC 6 cut(s) 356, 387, 515, 528, 801, 1326
AcsI RAATTY 1 cut(s) 569
AcuI CTGAAG 2 cut(s) 167, 219
AfaI GTAC 2 cut(s) 321, 600
AfiI CCNNNNNNNGG 4 cut(s) 241, 371, 565, 1132
AflII CTTAAG 1 cut(s) 605
AflIII ACRYGT 1 cut(s) 885
AgeI ACCGGT 1 cut(s) 322
AluBI AGCT 8 cut(s) 197, 292, 392, 491, 505, 563, 1067, 1259
AluI AGCT 8 cut(s) 197, 292, 392, 491, 505, 563, 1067, 1259
Alw21I GWGCWC 2 cut(s) 565, 703
Alw26I GTCTC 2 cut(s) 192, 362
Alw44I GTGCAC 1 cut(s) 699
AlwI GGATC 6 cut(s) 356, 387, 515, 528, 801, 1326
AlwNI CAGNNNCTG 1 cut(s) 1315
Ama87I CYCGRG 1 cut(s) 624
AoxI GGCC 1 cut(s) 162
ApaLI GTGCAC 1 cut(s) 699
ApeKI GCWGC 3 cut(s) 152, 1076, 1079
ApoI RAATTY 1 cut(s) 569
AsiGI ACCGGT 1 cut(s) 322
Asp700I GAANNNNTTC 1 cut(s) 228
AsuHPI GGTGA 5 cut(s) 69, 91, 256, 323, 854
AsuNHI GCTAGC 1 cut(s) 491
AvaI CYCGRG 1 cut(s) 624
BaeGI GKGCMC 3 cut(s) 476, 652, 703
BaeI ACNNNNGTAYC 2 cut(s) 1041, 1074
BamHI GGATCC 1 cut(s) 520
BanII GRGCYC 1 cut(s) 565
BbsI GAAGAC 2 cut(s) 207, 1215
Bbv12I GWGCWC 2 cut(s) 565, 703
BbvI GCAGC 3 cut(s) 164, 1088, 1091
BccI CCATC 1 cut(s) 1100
BceAI ACGGC 1 cut(s) 219
BciVI GTATCC 1 cut(s) 1042
BcoDI GTCTC 2 cut(s) 192, 362
BfaI CTAG 5 cut(s) 240, 263, 492, 1038, 1223
BfmI CTRYAG 1 cut(s) 515
BfrI CTTAAG 1 cut(s) 605
BfuI GTATCC 1 cut(s) 1042
BglI GCCNNNNNGGC 1 cut(s) 161
BisI GCNGC 3 cut(s) 153, 1077, 1080
BlsI GCNGC 3 cut(s) 154, 1078, 1081
BmeT110I CYCGRG 1 cut(s) 624
BmiI GGNNCC 1 cut(s) 522
BmrI ACTGGG 2 cut(s) 453, 471
BmsI GCATC 4 cut(s) 662, 882, 1167, 1228
BmtI GCTAGC 1 cut(s) 495
BmuI ACTGGG 2 cut(s) 453, 471
BpiI GAAGAC 2 cut(s) 207, 1215
BsaAI YACGTR 1 cut(s) 959
BsaBI GATNNNNATC 3 cut(s) 525, 854, 1323
BsaJI CCNNGG 3 cut(s) 159, 296, 926
BsaWI WCCGGW 2 cut(s) 322, 1281
Bsc4I CCNNNNNNNGG 4 cut(s) 241, 371, 565, 1132
Bse118I RCCGGY 1 cut(s) 322
Bse1I ACTGG 3 cut(s) 459, 477, 1320
Bse3DI GCAATG 2 cut(s) 1071, 1088
Bse8I GATNNNNATC 3 cut(s) 525, 854, 1323
BseDI CCNNGG 3 cut(s) 159, 296, 926
BseGI GGATG 5 cut(s) 25, 223, 306, 937, 1117
BseJI GATNNNNATC 3 cut(s) 525, 854, 1323
BseLI CCNNNNNNNGG 4 cut(s) 241, 371, 565, 1132
BseMI GCAATG 2 cut(s) 1071, 1088
BseMII CTCAG 4 cut(s) 204, 624, 644, 1011
BseNI ACTGG 3 cut(s) 459, 477, 1320
BseRI GAGGAG 2 cut(s) 512, 515
BseSI GKGCMC 3 cut(s) 476, 652, 703
BseXI GCAGC 3 cut(s) 164, 1088, 1091
BsgI GTGCAG 1 cut(s) 483
BshFI GGCC 1 cut(s) 164
BshTI ACCGGT 1 cut(s) 322
BsiHKAI GWGCWC 2 cut(s) 565, 703
BsiHKCI CYCGRG 1 cut(s) 624
BsiSI CCGG 2 cut(s) 323, 1282
BslI CCNNNNNNNGG 4 cut(s) 241, 371, 565, 1132
BsmAI GTCTC 2 cut(s) 192, 362
BsmI GAATGC 4 cut(s) 123, 129, 589, 1172
BsnI GGCC 1 cut(s) 164
BsoBI CYCGRG 1 cut(s) 624
Bsp1286I GDGCHC 4 cut(s) 476, 565, 652, 703
Bsp143I GATC 6 cut(s) 361, 379, 415, 520, 793, 1318
Bsp19I CCATGG 2 cut(s) 296, 926
BspACI CCGC 1 cut(s) 784
BspANI GGCC 1 cut(s) 164
BspCNI CTCAG 4 cut(s) 205, 625, 645, 1010
BspLI GGNNCC 1 cut(s) 522
BspOI GCTAGC 1 cut(s) 495
BspPI GGATC 6 cut(s) 356, 387, 515, 528, 801, 1326
BspTI CTTAAG 1 cut(s) 605
BsrDI GCAATG 2 cut(s) 1071, 1088
BsrFI RCCGGY 1 cut(s) 322
BsrI ACTGG 3 cut(s) 459, 477, 1320
BssAI RCCGGY 1 cut(s) 322
BssECI CCNNGG 3 cut(s) 159, 296, 926
BssMI GATC 6 cut(s) 361, 379, 415, 520, 793, 1318
BssT1I CCWWGG 2 cut(s) 296, 926
Bst6I CTCTTC 2 cut(s) 18, 24
BstAFI CTTAAG 1 cut(s) 605
BstBAI YACGTR 1 cut(s) 959
BstC8I GCNNGC 4 cut(s) 394, 493, 683, 1182
BstDEI CTNAG 6 cut(s) 213, 383, 633, 653, 997, 1203
BstDSI CCRYGG 2 cut(s) 296, 926
BstEII GGTNACC 1 cut(s) 329
BstF5I GGATG 5 cut(s) 25, 223, 306, 937, 1117
BstKTI GATC 6 cut(s) 364, 382, 418, 523, 796, 1321
BstMAI GTCTC 2 cut(s) 192, 362
BstMBI GATC 6 cut(s) 361, 379, 415, 520, 793, 1318
BstMWI GCNNNNNNNGC 3 cut(s) 152, 161, 1073
BstNSI RCATGY 3 cut(s) 685, 745, 889
BstPI GGTNACC 1 cut(s) 329
BstSFI CTRYAG 1 cut(s) 515
BstSLI GKGCMC 3 cut(s) 476, 652, 703
BstV1I GCAGC 3 cut(s) 164, 1088, 1091
BstV2I GAAGAC 2 cut(s) 207, 1215
BstX2I RGATCY 3 cut(s) 379, 520, 1318
BstYI RGATCY 3 cut(s) 379, 520, 1318
BsuI GTATCC 1 cut(s) 1042
BsuRI GGCC 1 cut(s) 164
BtgI CCRYGG 2 cut(s) 296, 926
BtsCI GGATG 5 cut(s) 25, 223, 306, 937, 1117
BtsI GCAGTG 1 cut(s) 34
BtsIMutI CAGTG 4 cut(s) 34, 466, 650, 1313
Cac8I GCNNGC 4 cut(s) 394, 493, 683, 1182
CaiI CAGNNNCTG 1 cut(s) 1315
Cfr10I RCCGGY 1 cut(s) 322
CseI GACGC 1 cut(s) 1258
Csp6I GTAC 2 cut(s) 320, 599
CspAI ACCGGT 1 cut(s) 322
CviAII CATG 8 cut(s) 297, 682, 742, 865, 886, 927, 1132, 1306
CviQI GTAC 2 cut(s) 320, 599
DdeI CTNAG 6 cut(s) 213, 383, 633, 653, 997, 1203
DpnI GATC 6 cut(s) 363, 381, 417, 522, 795, 1320
DpnII GATC 6 cut(s) 361, 379, 415, 520, 793, 1318
DrdI GACNNNNNNGTC 1 cut(s) 1067
DseDI GACNNNNNNGTC 1 cut(s) 1067
Eam1104I CTCTTC 2 cut(s) 18, 24
EarI CTCTTC 2 cut(s) 18, 24
Ecl136II GAGCTC 1 cut(s) 563
Eco130I CCWWGG 2 cut(s) 296, 926
Eco147I AGGCCT 1 cut(s) 164
Eco24I GRGCYC 1 cut(s) 565
Eco32I GATATC 1 cut(s) 346
Eco53kI GAGCTC 1 cut(s) 563
Eco57I CTGAAG 2 cut(s) 167, 219
Eco88I CYCGRG 1 cut(s) 624
Eco91I GGTNACC 1 cut(s) 329
EcoICRI GAGCTC 1 cut(s) 563
EcoO65I GGTNACC 1 cut(s) 329
EcoRV GATATC 1 cut(s) 346
EcoT14I CCWWGG 2 cut(s) 296, 926
EcoT22I ATGCAT 2 cut(s) 412, 677
EcoT38I GRGCYC 1 cut(s) 565
ErhI CCWWGG 2 cut(s) 296, 926
FaeI CATG 8 cut(s) 300, 685, 745, 868, 889, 930, 1135, 1309
FalI AAGNNNNNCTT 2 cut(s) 376, 408
FatI CATG 8 cut(s) 296, 681, 741, 864, 885, 926, 1131, 1305
Fnu4HI GCNGC 3 cut(s) 153, 1077, 1080
FokI GGATG 5 cut(s) 12, 230, 313, 944, 1124
FriOI GRGCYC 1 cut(s) 565
Fsp4HI GCNGC 3 cut(s) 153, 1077, 1080
FspBI CTAG 5 cut(s) 240, 263, 492, 1038, 1223
GluI GCNGC 3 cut(s) 153, 1077, 1080
HaeIII GGCC 1 cut(s) 164
HapII CCGG 2 cut(s) 323, 1282
HgaI GACGC 1 cut(s) 1258
Hin1II CATG 8 cut(s) 300, 685, 745, 868, 889, 930, 1135, 1309
HincII GTYRAC 2 cut(s) 276, 1060
HindII GTYRAC 2 cut(s) 276, 1060
HinfI GANTC 8 cut(s) 90, 203, 629, 639, 713, 922, 971, 1226
HpaI GTTAAC 1 cut(s) 276
HpaII CCGG 2 cut(s) 323, 1282
HphI GGTGA 5 cut(s) 69, 91, 256, 323, 854
Hpy166II GTNNAC 5 cut(s) 276, 599, 701, 872, 1060
Hpy188I TCNGA 5 cut(s) 214, 511, 634, 1105, 1346
Hpy8I GTNNAC 5 cut(s) 276, 599, 701, 872, 1060
HpyAV CCTTC 3 cut(s) 407, 766, 985
HpyCH4IV ACGT 1 cut(s) 958
HpyF10VI GCNNNNNNNGC 3 cut(s) 152, 161, 1073
HpyF3I CTNAG 6 cut(s) 213, 383, 633, 653, 997, 1203
HpySE526I ACGT 1 cut(s) 958
Hsp92II CATG 8 cut(s) 300, 685, 745, 868, 889, 930, 1135, 1309
KspAI GTTAAC 1 cut(s) 276
Kzo9I GATC 6 cut(s) 361, 379, 415, 520, 793, 1318
LmnI GCTCC 3 cut(s) 297, 502, 1064
Lsp1109I GCAGC 3 cut(s) 164, 1088, 1091
LweI GCATC 4 cut(s) 662, 882, 1167, 1228
MaeI CTAG 5 cut(s) 240, 263, 492, 1038, 1223
MaeII ACGT 1 cut(s) 958
MaeIII GTNAC 1 cut(s) 329
MalI GATC 6 cut(s) 363, 381, 417, 522, 795, 1320
MboI GATC 6 cut(s) 361, 379, 415, 520, 793, 1318
MboII GAAGA 6 cut(s) 5, 11, 212, 903, 1175, 1220
MflI RGATCY 3 cut(s) 379, 520, 1318
MhlI GDGCHC 4 cut(s) 476, 565, 652, 703
MlyI GAGTC 2 cut(s) 197, 707
Mph1103I ATGCAT 2 cut(s) 412, 677
MroXI GAANNNNTTC 1 cut(s) 228
MseI TTAA 6 cut(s) 141, 275, 606, 612, 789, 834
MslI CAYNNNNRTG 3 cut(s) 594, 680, 890
MspCI CTTAAG 1 cut(s) 605
MspI CCGG 2 cut(s) 323, 1282
Mva1269I GAATGC 4 cut(s) 123, 129, 589, 1172
MwoI GCNNNNNNNGC 3 cut(s) 152, 161, 1073
NcoI CCATGG 2 cut(s) 296, 926
NdeII GATC 6 cut(s) 361, 379, 415, 520, 793, 1318
NheI GCTAGC 1 cut(s) 491
NlaIII CATG 8 cut(s) 300, 685, 745, 868, 889, 930, 1135, 1309
NlaIV GGNNCC 1 cut(s) 522
NmeAIII GCCGAG 1 cut(s) 584
NmuCI GTSAC 1 cut(s) 329
NsiI ATGCAT 2 cut(s) 412, 677
NspI RCATGY 3 cut(s) 685, 745, 889
PaeI GCATGC 1 cut(s) 685
PaeR7I CTCGAG 1 cut(s) 624
PceI AGGCCT 1 cut(s) 164
PciI ACATGT 1 cut(s) 885
PcsI WCGNNNNNNNCGW 1 cut(s) 622
PctI GAATGC 4 cut(s) 123, 129, 589, 1172
PdmI GAANNNNTTC 1 cut(s) 228
PfeI GAWTC 6 cut(s) 90, 629, 639, 922, 971, 1226
PinAI ACCGGT 1 cut(s) 322
PkrI GCNGC 3 cut(s) 154, 1078, 1081
PleI GAGTC 2 cut(s) 197, 707
PpsI GAGTC 2 cut(s) 197, 707
Ppu21I YACGTR 1 cut(s) 959
PscI ACATGT 1 cut(s) 885
Psp124BI GAGCTC 1 cut(s) 565
PspEI GGTNACC 1 cut(s) 329
PspN4I GGNNCC 1 cut(s) 522
PsrI GAACNNNNNNTAC 2 cut(s) 1078, 1110
PstNI CAGNNNCTG 1 cut(s) 1315
PsuI RGATCY 3 cut(s) 379, 520, 1318
RsaI GTAC 2 cut(s) 321, 600
RsaNI GTAC 2 cut(s) 320, 599
RseI CAYNNNNRTG 3 cut(s) 594, 680, 890
SacI GAGCTC 1 cut(s) 565
SaqAI TTAA 6 cut(s) 141, 275, 606, 612, 789, 834
SatI GCNGC 3 cut(s) 153, 1077, 1080
Sau3AI GATC 6 cut(s) 361, 379, 415, 520, 793, 1318
SchI GAGTC 2 cut(s) 197, 707
SduI GDGCHC 4 cut(s) 476, 565, 652, 703
SfaNI GCATC 4 cut(s) 662, 882, 1167, 1228
SfcI CTRYAG 1 cut(s) 515
Sfr274I CTCGAG 1 cut(s) 624
SlaI CTCGAG 1 cut(s) 624
SmiMI CAYNNNNRTG 3 cut(s) 594, 680, 890
SmlI CTYRAG 2 cut(s) 605, 624
SmoI CTYRAG 2 cut(s) 605, 624
SphI GCATGC 1 cut(s) 685
SseBI AGGCCT 1 cut(s) 164
SsiI CCGC 1 cut(s) 784
SspI AATATT 2 cut(s) 339, 1338
SspMI CTAG 5 cut(s) 240, 263, 492, 1038, 1223
SstI GAGCTC 1 cut(s) 565
StuI AGGCCT 1 cut(s) 164
StyI CCWWGG 2 cut(s) 296, 926
TaiI ACGT 1 cut(s) 961
TaqI TCGA 2 cut(s) 625, 1296
TfiI GAWTC 6 cut(s) 90, 629, 639, 922, 971, 1226
Tru1I TTAA 6 cut(s) 141, 275, 606, 612, 789, 834
Tru9I TTAA 6 cut(s) 141, 275, 606, 612, 789, 834
TscAI CASTG 4 cut(s) 41, 466, 657, 1320
TseFI GTSAC 1 cut(s) 329
TseI GCWGC 3 cut(s) 152, 1076, 1079
Tsp45I GTSAC 1 cut(s) 329
TspDTI ATGAA 4 cut(s) 11, 631, 903, 953
TspGWI ACGGA 1 cut(s) 1077
TspRI CASTG 4 cut(s) 41, 466, 657, 1320
Vha464I CTTAAG 1 cut(s) 605
VneI GTGCAC 1 cut(s) 699
XapI RAATTY 1 cut(s) 569
XceI RCATGY 3 cut(s) 685, 745, 889
XcmI CCANNNNNNNNNTGG 1 cut(s) 372
XhoI CTCGAG 1 cut(s) 624
XmnI GAANNNNTTC 1 cut(s) 228
XspI CTAG 5 cut(s) 240, 263, 492, 1038, 1223
Zsp2I ATGCAT 2 cut(s) 412, 677
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.