Prupe.4G271100_v2.0.a1

Presenilin enhancer-2 subunit of gamma secretase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0010505
Physical Location & Seq
Forward (+)
22060302 .. 22065739
5438 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G271100.1

Sequence Viewer

Length: 426 bp
ATGGAAGCCTCACTGGGTAATGGCGAAATGCCAAACCAGAACCCTATCAATCCAAGAAGTAACGTGACTCTGGTTCCGTCGTCTTCATCGCCGGTGTGGCCCACCATTGACGGCCCACTGGGGCTCTCTGAGGAAGACTCTGTGAGTTACGCGCGTAGATTCTACAAGTTCGGGTTCGCTCTGCTCCCCTGGCTTTGGGCCCTCAACTGCTTCTACTTCTGGCCCGCTCTCCGCCACTCCCGCTCCTTCCCTCGCATTCACCACTATGTTTTGAGATCAGCAGTTGGGCTCACAGTATTTACTGTTCTTCTCGCATCATGGGCATTTACGTTTGTCATCGGAGGGGAGCGCCTGTTCGGACATGTTTGGGATCAATTGGTGATGTACAATCTTGCTGACAGACTAGGCTTGACAGGTTGGAGCTAA

Protein Analysis

142

Amino Acids

15.99

Weight (kDa)

7.97

Isoelectric Point (pI)

60.66

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010505)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G09310
fragaria_vesca FvH4_2g08820 FvH4_2g08820 FvH4_2g08820 FvH4_2g08820 FvH4_2g08820 FvH4_3g04510
malus_domestica MD02G1262600.v1.1 MD07G1057500.v1.1
prunus_persica Prupe.4G271100_v2.0.a1
pyrus_communis pycom02g22520
rosa_chinensis RchiOBHm_Chr6g0264391
rosa_laevigata RLG00000014257
rosa_multiflora Rmu_sc0004470.1_g000020
rosa_roxburghii Rroxscaffold_176G00431770
rosa_rugosa Rorug06G0012000 Rorug06G0012100
rosa_samantha Rh6BG129000 Rh6CG127000 Rh6DG114500
rosa_wichuraiana Rw6G011520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 2 cut(s) 227, 243
AccII CGCG 2 cut(s) 152, 154
AciI CCGC 3 cut(s) 225, 232, 241
AclWI GGATC 1 cut(s) 378
AfaI GTAC 1 cut(s) 386
AfiI CCNNNNNNNGG 1 cut(s) 195
AflIII ACRYGT 1 cut(s) 361
AjnI CCWGG 1 cut(s) 188
AloI GAACNNNNNNTCC 2 cut(s) 338, 370
AluBI AGCT 1 cut(s) 423
AluI AGCT 1 cut(s) 423
AlwI GGATC 1 cut(s) 378
AoxI GGCC 4 cut(s) 98, 112, 198, 221
ApaI GGGCCC 1 cut(s) 202
AspLEI GCGC 2 cut(s) 154, 351
AspS9I GGNCC 5 cut(s) 99, 113, 198, 199, 222
AsuHPI GGTGA 2 cut(s) 251, 391
BaeGI GKGCMC 1 cut(s) 202
BanII GRGCYC 3 cut(s) 126, 202, 291
BbsI GAAGAC 2 cut(s) 75, 141
BceAI ACGGC 1 cut(s) 127
BciT130I CCWGG 1 cut(s) 190
BfaI CTAG 1 cut(s) 404
BfoI RGCGCY 1 cut(s) 352
BglI GCCNNNNNGGC 1 cut(s) 97
Bme1390I CCNGG 1 cut(s) 190
BmgT120I GGNCC 5 cut(s) 99, 113, 198, 199, 222
BmiI GGNNCC 2 cut(s) 75, 200
BmrFI CCNGG 1 cut(s) 190
BmrI ACTGGG 2 cut(s) 23, 128
BmsI GCATC 1 cut(s) 323
BmuI ACTGGG 2 cut(s) 23, 128
BpiI GAAGAC 2 cut(s) 75, 141
BplI GAGNNNNNCTC 2 cut(s) 122, 154
BsaJI CCNNGG 1 cut(s) 188
BsaXI ACNNNNNCTCC 4 cut(s) 227, 257, 338, 368
Bsc4I CCNNNNNNNGG 1 cut(s) 195
Bse118I RCCGGY 1 cut(s) 91
Bse1I ACTGG 2 cut(s) 18, 123
BseBI CCWGG 1 cut(s) 190
BseDI CCNNGG 1 cut(s) 188
BseLI CCNNNNNNNGG 1 cut(s) 195
BseMII CTCAG 1 cut(s) 120
BseNI ACTGG 2 cut(s) 18, 123
BseSI GKGCMC 1 cut(s) 202
Bsh1236I CGCG 2 cut(s) 152, 154
BshFI GGCC 4 cut(s) 100, 114, 200, 223
BsiSI CCGG 1 cut(s) 92
BslI CCNNNNNNNGG 1 cut(s) 195
BsmI GAATGC 1 cut(s) 255
BsnI GGCC 4 cut(s) 100, 114, 200, 223
Bsp120I GGGCCC 1 cut(s) 198
Bsp1286I GDGCHC 3 cut(s) 126, 202, 291
Bsp1407I TGTACA 1 cut(s) 384
Bsp143I GATC 2 cut(s) 275, 370
BspACI CCGC 3 cut(s) 225, 232, 241
BspANI GGCC 4 cut(s) 100, 114, 200, 223
BspCNI CTCAG 1 cut(s) 121
BspFNI CGCG 2 cut(s) 152, 154
BspLI GGNNCC 2 cut(s) 75, 200
BspPI GGATC 1 cut(s) 378
BsrBI CCGCTC 2 cut(s) 227, 243
BsrFI RCCGGY 1 cut(s) 91
BsrGI TGTACA 1 cut(s) 384
BsrI ACTGG 2 cut(s) 18, 123
BssAI RCCGGY 1 cut(s) 91
BssECI CCNNGG 1 cut(s) 188
BssMI GATC 2 cut(s) 275, 370
Bst2UI CCWGG 1 cut(s) 190
Bst4CI ACNGT 2 cut(s) 295, 304
BstAUI TGTACA 1 cut(s) 384
BstC8I GCNNGC 1 cut(s) 225
BstDEI CTNAG 1 cut(s) 129
BstFNI CGCG 2 cut(s) 152, 154
BstH2I RGCGCY 1 cut(s) 352
BstHHI GCGC 2 cut(s) 154, 351
BstKTI GATC 2 cut(s) 278, 373
BstMBI GATC 2 cut(s) 275, 370
BstMWI GCNNNNNNNGC 4 cut(s) 97, 190, 240, 320
BstNI CCWGG 1 cut(s) 190
BstNSI RCATGY 1 cut(s) 365
BstSCI CCNGG 1 cut(s) 188
BstSLI GKGCMC 1 cut(s) 202
BstUI CGCG 2 cut(s) 152, 154
BstV2I GAAGAC 2 cut(s) 75, 141
BsuRI GGCC 4 cut(s) 100, 114, 200, 223
BtgZI GCGATG 1 cut(s) 72
BtsIMutI CAGTG 2 cut(s) 11, 116
Cac8I GCNNGC 1 cut(s) 225
CfoI GCGC 2 cut(s) 154, 351
Cfr10I RCCGGY 1 cut(s) 91
Cfr13I GGNCC 5 cut(s) 99, 113, 198, 199, 222
Csp6I GTAC 1 cut(s) 385
CviAII CATG 2 cut(s) 318, 362
CviQI GTAC 1 cut(s) 385
DdeI CTNAG 1 cut(s) 129
DpnI GATC 2 cut(s) 277, 372
DpnII GATC 2 cut(s) 275, 370
EciI GGCGGA 1 cut(s) 221
Eco24I GRGCYC 3 cut(s) 126, 202, 291
EcoO109I RGGNCCY 1 cut(s) 199
EcoRII CCWGG 1 cut(s) 188
EcoT38I GRGCYC 3 cut(s) 126, 202, 291
FaeI CATG 2 cut(s) 321, 365
FaiI YATR 3 cut(s) 267, 319, 363
FatI CATG 2 cut(s) 317, 361
FauI CCCGC 2 cut(s) 232, 248
FriOI GRGCYC 3 cut(s) 126, 202, 291
FspBI CTAG 1 cut(s) 404
GlaI GCGC 2 cut(s) 153, 350
HaeII RGCGCY 1 cut(s) 352
HaeIII GGCC 4 cut(s) 100, 114, 200, 223
HapII CCGG 1 cut(s) 92
HhaI GCGC 2 cut(s) 154, 351
Hin1II CATG 2 cut(s) 321, 365
Hin6I GCGC 2 cut(s) 152, 349
HinP1I GCGC 2 cut(s) 152, 349
HinfI GANTC 3 cut(s) 67, 137, 159
HpaII CCGG 1 cut(s) 92
HphI GGTGA 2 cut(s) 251, 391
Hpy188I TCNGA 3 cut(s) 130, 341, 359
Hpy99I CGWCG 1 cut(s) 82
HpyAV CCTTC 1 cut(s) 256
HpyCH4III ACNGT 2 cut(s) 295, 304
HpyCH4IV ACGT 2 cut(s) 63, 329
HpyF10VI GCNNNNNNNGC 4 cut(s) 97, 190, 240, 320
HpyF3I CTNAG 1 cut(s) 129
HpySE526I ACGT 2 cut(s) 63, 329
Hsp92II CATG 2 cut(s) 321, 365
HspAI GCGC 2 cut(s) 152, 349
Kzo9I GATC 2 cut(s) 275, 370
LmnI GCTCC 4 cut(s) 189, 248, 346, 420
LpnPI CCDG 9 cut(s) 50, 56, 104, 105, 175, 202, 205, 365, 399
LweI GCATC 1 cut(s) 323
MaeI CTAG 1 cut(s) 404
MaeII ACGT 2 cut(s) 63, 329
MaeIII GTNAC 3 cut(s) 59, 64, 146
MalI GATC 2 cut(s) 277, 372
MbiI CCGCTC 2 cut(s) 227, 243
MboI GATC 2 cut(s) 275, 370
MboII GAAGA 3 cut(s) 75, 146, 299
MfeI CAATTG 1 cut(s) 374
MhlI GDGCHC 3 cut(s) 126, 202, 291
MluCI AATT 1 cut(s) 374
MlyI GAGTC 2 cut(s) 61, 131
MmeI TCCRAC 1 cut(s) 398
MnlI CCTC 5 cut(s) 19, 124, 212, 261, 335
MslI CAYNNNNRTG 1 cut(s) 264
MspI CCGG 1 cut(s) 92
MspR9I CCNGG 1 cut(s) 190
MunI CAATTG 1 cut(s) 374
Mva1269I GAATGC 1 cut(s) 255
MvaI CCWGG 1 cut(s) 190
MvnI CGCG 2 cut(s) 152, 154
MwoI GCNNNNNNNGC 4 cut(s) 97, 190, 240, 320
NdeII GATC 2 cut(s) 275, 370
NlaIII CATG 2 cut(s) 321, 365
NlaIV GGNNCC 2 cut(s) 75, 200
NmuCI GTSAC 1 cut(s) 64
NspI RCATGY 1 cut(s) 365
PciI ACATGT 1 cut(s) 361
PctI GAATGC 1 cut(s) 255
PfeI GAWTC 1 cut(s) 159
PleI GAGTC 2 cut(s) 61, 131
PpsI GAGTC 2 cut(s) 61, 131
PscI ACATGT 1 cut(s) 361
Psp6I CCWGG 1 cut(s) 188
PspGI CCWGG 1 cut(s) 188
PspN4I GGNNCC 2 cut(s) 75, 200
PspOMI GGGCCC 1 cut(s) 198
PspPI GGNCC 5 cut(s) 99, 113, 198, 199, 222
PsrI GAACNNNNNNTAC 2 cut(s) 288, 320
RsaI GTAC 1 cut(s) 386
RsaNI GTAC 1 cut(s) 385
RseI CAYNNNNRTG 1 cut(s) 264
Sau3AI GATC 2 cut(s) 275, 370
Sau96I GGNCC 5 cut(s) 99, 113, 198, 199, 222
SchI GAGTC 2 cut(s) 61, 131
ScrFI CCNGG 1 cut(s) 190
SduI GDGCHC 3 cut(s) 126, 202, 291
SetI ASST 4 cut(s) 66, 332, 418, 425
SfaNI GCATC 1 cut(s) 323
SgrAI CRCCGGYG 1 cut(s) 91
SmiMI CAYNNNNRTG 1 cut(s) 264
Sse9I AATT 1 cut(s) 374
SsiI CCGC 3 cut(s) 225, 232, 241
SspMI CTAG 1 cut(s) 404
StyD4I CCNGG 1 cut(s) 188
TaaI ACNGT 2 cut(s) 295, 304
TaiI ACGT 2 cut(s) 66, 332
TasI AATT 1 cut(s) 374
TatI WGTACW 1 cut(s) 384
TfiI GAWTC 1 cut(s) 159
TscAI CASTG 2 cut(s) 18, 123
TseFI GTSAC 1 cut(s) 64
Tsp45I GTSAC 1 cut(s) 64
TspDTI ATGAA 1 cut(s) 75
TspGWI ACGGA 1 cut(s) 66
TspRI CASTG 2 cut(s) 18, 123
XceI RCATGY 1 cut(s) 365
XspI CTAG 1 cut(s) 404
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.