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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT4G10930 AT4G10930 |
| fragaria_vesca | FvH4_5g04850 FvH4_5g04850 FvH4_5g04850 |
| malus_domestica | MD06G1109300.v1.1 MD12G1080900.v1.1 MD14G1130300.v1.1 |
| prunus_persica | Prupe.5G122300_v2.0.a1 Prupe.5G122300_v2.0.a1 Prupe.5G122300_v2.0.a1 |
| pyrus_communis | pycom06g10260 pycom06g10270 pycom06g10280 |
| rosa_chinensis | RchiOBHm_Chr7g0194241 |
| rosa_laevigata | RLG00000004207 |
| rosa_multiflora | Rmu_co8489007.1_g000001 Rmu_sc0000901.1_g000004 Rmu_sc0009433.1_g000017 |
| rosa_roxburghii | Rroxscaffold_3G00261470 |
| rosa_rugosa | Rorug07G0014700 |
| rosa_samantha | Rh7AG142000 Rh7BG142400 Rh7CG146100 Rh7DG143400 |
| rosa_wichuraiana | Rw7G012140 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 2 cut(s) | 1417, 3433 |
| Acc65I | GGTACC | 1 cut(s) | 495 |
| AccB1I | GGYRCC | 3 cut(s) | 495, 2683, 3265 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 847, 890 |
| AccBSI | CCGCTC | 1 cut(s) | 2125 |
| AccII | CGCG | 4 cut(s) | 648, 755, 1992, 2447 |
| AccIII | TCCGGA | 1 cut(s) | 1028 |
| AciI | CCGC | 5 cut(s) | 755, 2125, 2447, 2586, 3869 |
| AclWI | GGATC | 6 cut(s) | 515, 991, 2773, 2999, 3596, 4046 |
| AcsI | RAATTY | 7 cut(s) | 242, 417, 946, 1959, 2018, 2317, 2677 |
| AcuI | CTGAAG | 8 cut(s) | 1176, 1239, 1251, 2451, 2538, 2940, 3281, 3369 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 847, 890, 2402, 3260, 3982, 4039 |
| AflIII | ACRYGT | 2 cut(s) | 116, 257 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 805 |
| AjnI | CCWGG | 8 cut(s) | 801, 1312, 2162, 2564, 2886, 3168, 3544, 3751 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 3201, 3233 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 1562, 1594 |
| Alw21I | GWGCWC | 5 cut(s) | 898, 2628, 2751, 3445, 3861 |
| AlwI | GGATC | 6 cut(s) | 515, 991, 2773, 2999, 3596, 4046 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 1753 |
| Ama87I | CYCGRG | 1 cut(s) | 860 |
| Aor13HI | TCCGGA | 1 cut(s) | 1028 |
| AoxI | GGCC | 3 cut(s) | 628, 854, 3131 |
| ApoI | RAATTY | 7 cut(s) | 242, 417, 946, 1959, 2018, 2317, 2677 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 1219, 1251 |
| AseI | ATTAAT | 1 cut(s) | 2823 |
| Asp718I | GGTACC | 1 cut(s) | 495 |
| AspLEI | GCGC | 3 cut(s) | 958, 1808, 1994 |
| AspS9I | GGNCC | 9 cut(s) | 629, 799, 855, 1859, 1999, 2005, 3104, 3131, 3512 |
| AsuC2I | CCSGG | 3 cut(s) | 861, 862, 2003 |
| AsuHPI | GGTGA | 7 cut(s) | 297, 719, 1628, 3485, 3737, 4001, 4009 |
| AsuII | TTCGAA | 2 cut(s) | 421, 1600 |
| AsuNHI | GCTAGC | 1 cut(s) | 2647 |
| AvaI | CYCGRG | 1 cut(s) | 860 |
| AvaII | GGWCC | 6 cut(s) | 799, 1859, 1999, 2005, 3104, 3512 |
| AxyI | CCTNAGG | 1 cut(s) | 525 |
| BaeGI | GKGCMC | 2 cut(s) | 533, 3268 |
| BanI | GGYRCC | 3 cut(s) | 495, 2683, 3265 |
| BanII | GRGCYC | 3 cut(s) | 898, 1118, 2932 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 1774, 1806 |
| BbsI | GAAGAC | 6 cut(s) | 1454, 1910, 3032, 3216, 3350, 3788 |
| Bbv12I | GWGCWC | 5 cut(s) | 898, 2628, 2751, 3445, 3861 |
| BbvCI | CCTCAGC | 1 cut(s) | 1719 |
| BccI | CCATC | 8 cut(s) | 370, 395, 401, 974, 1379, 3095, 3145, 3685 |
| BceAI | ACGGC | 2 cut(s) | 729, 1835 |
| BciT130I | CCWGG | 8 cut(s) | 803, 1314, 2164, 2566, 2888, 3170, 3546, 3753 |
| BclI | TGATCA | 1 cut(s) | 252 |
| BcnI | CCSGG | 3 cut(s) | 861, 862, 2003 |
| BfaI | CTAG | 7 cut(s) | 905, 2366, 2421, 2648, 2864, 2922, 3323 |
| BfmI | CTRYAG | 3 cut(s) | 687, 2790, 3915 |
| BglI | GCCNNNNNGGC | 2 cut(s) | 627, 1844 |
| BglII | AGATCT | 2 cut(s) | 3308, 3325 |
| BmcAI | AGTACT | 2 cut(s) | 976, 2937 |
| Bme18I | GGWCC | 6 cut(s) | 799, 1859, 1999, 2005, 3104, 3512 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 805 |
| BmeT110I | CYCGRG | 1 cut(s) | 860 |
| BmgT120I | GGNCC | 9 cut(s) | 629, 799, 855, 1859, 1999, 2005, 3104, 3131, 3512 |
| BmrI | ACTGGG | 2 cut(s) | 2682, 2927 |
| BmtI | GCTAGC | 1 cut(s) | 2651 |
| BmuI | ACTGGG | 2 cut(s) | 2682, 2927 |
| BpiI | GAAGAC | 6 cut(s) | 1454, 1910, 3032, 3216, 3350, 3788 |
| BpmI | CTGGAG | 1 cut(s) | 824 |
| Bpu10I | CCTNAGC | 3 cut(s) | 897, 1719, 2401 |
| Bpu14I | TTCGAA | 2 cut(s) | 421, 1600 |
| BpuEI | CTTGAG | 2 cut(s) | 2776, 3398 |
| BpuMI | CCSGG | 3 cut(s) | 861, 862, 2003 |
| Bsa29I | ATCGAT | 1 cut(s) | 144 |
| BsaAI | YACGTR | 1 cut(s) | 1940 |
| BsaBI | GATNNNNATC | 2 cut(s) | 453, 3159 |
| BsaI | GGTCTC | 2 cut(s) | 1403, 2665 |
| BsaJI | CCNNGG | 6 cut(s) | 859, 860, 861, 1623, 1737, 2886 |
| BsaWI | WCCGGW | 1 cut(s) | 1028 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 2919, 2949 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 847, 890, 2402, 3260, 3982, 4039 |
| Bse118I | RCCGGY | 1 cut(s) | 3035 |
| Bse21I | CCTNAGG | 1 cut(s) | 525 |
| Bse3DI | GCAATG | 3 cut(s) | 24, 94, 1923 |
| Bse8I | GATNNNNATC | 2 cut(s) | 453, 3159 |
| BseAI | TCCGGA | 1 cut(s) | 1028 |
| BseBI | CCWGG | 8 cut(s) | 803, 1314, 2164, 2566, 2888, 3170, 3546, 3753 |
| BseCI | ATCGAT | 1 cut(s) | 144 |
| BseDI | CCNNGG | 6 cut(s) | 859, 860, 861, 1623, 1737, 2886 |
| BseJI | GATNNNNATC | 2 cut(s) | 453, 3159 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 847, 890, 2402, 3260, 3982, 4039 |
| BseMI | GCAATG | 3 cut(s) | 24, 94, 1923 |
| BseRI | GAGGAG | 5 cut(s) | 1457, 1580, 1723, 3045, 3706 |
| BseSI | GKGCMC | 2 cut(s) | 533, 3268 |
| BseYI | CCCAGC | 1 cut(s) | 879 |
| BsgI | GTGCAG | 2 cut(s) | 2133, 2601 |
| Bsh1236I | CGCG | 4 cut(s) | 648, 755, 1992, 2447 |
| Bsh1285I | CGRYCG | 1 cut(s) | 3795 |
| BshFI | GGCC | 3 cut(s) | 630, 856, 3133 |
| BshNI | GGYRCC | 3 cut(s) | 495, 2683, 3265 |
| BshVI | ATCGAT | 1 cut(s) | 144 |
| BsiEI | CGRYCG | 1 cut(s) | 3795 |
| BsiHKAI | GWGCWC | 5 cut(s) | 898, 2628, 2751, 3445, 3861 |
| BsiHKCI | CYCGRG | 1 cut(s) | 860 |
| BsiSI | CCGG | 4 cut(s) | 861, 1029, 2002, 3036 |
| BslFI | GGGAC | 5 cut(s) | 1981, 2012, 2018, 3090, 3439 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 847, 890, 2402, 3260, 3982, 4039 |
| BsmBI | CGTCTC | 4 cut(s) | 6, 309, 704, 2301 |
| BsmFI | GGGAC | 5 cut(s) | 1981, 2012, 2018, 3090, 3439 |
| BsnI | GGCC | 3 cut(s) | 630, 856, 3133 |
| Bso31I | GGTCTC | 2 cut(s) | 1403, 2665 |
| BsoBI | CYCGRG | 1 cut(s) | 860 |
| Bsp119I | TTCGAA | 2 cut(s) | 421, 1600 |
| Bsp1286I | GDGCHC | 9 cut(s) | 533, 898, 1118, 2628, 2751, 2932, 3268, 3445, 3861 |
| Bsp13I | TCCGGA | 1 cut(s) | 1028 |
| Bsp19I | CCATGG | 1 cut(s) | 1737 |
| Bsp68I | TCGCGA | 1 cut(s) | 648 |
| BspACI | CCGC | 5 cut(s) | 755, 2125, 2447, 2586, 3869 |
| BspANI | GGCC | 3 cut(s) | 630, 856, 3133 |
| BspDI | ATCGAT | 1 cut(s) | 144 |
| BspEI | TCCGGA | 1 cut(s) | 1028 |
| BspFNI | CGCG | 4 cut(s) | 648, 755, 1992, 2447 |
| BspHI | TCATGA | 1 cut(s) | 1704 |
| BspMAI | CTGCAG | 2 cut(s) | 2794, 3919 |
| BspOI | GCTAGC | 1 cut(s) | 2651 |
| BspPI | GGATC | 6 cut(s) | 515, 991, 2773, 2999, 3596, 4046 |
| BspQI | GCTCTTC | 1 cut(s) | 3471 |
| BspT104I | TTCGAA | 2 cut(s) | 421, 1600 |
| BspT107I | GGYRCC | 3 cut(s) | 495, 2683, 3265 |
| BspTNI | GGTCTC | 2 cut(s) | 1403, 2665 |
| BsrBI | CCGCTC | 1 cut(s) | 2125 |
| BsrDI | GCAATG | 3 cut(s) | 24, 94, 1923 |
| BsrFI | RCCGGY | 1 cut(s) | 3035 |
| BssAI | RCCGGY | 1 cut(s) | 3035 |
| BssECI | CCNNGG | 6 cut(s) | 859, 860, 861, 1623, 1737, 2886 |
| BssT1I | CCWWGG | 2 cut(s) | 1623, 1737 |
| Bst2UI | CCWGG | 8 cut(s) | 803, 1314, 2164, 2566, 2888, 3170, 3546, 3753 |
| Bst4CI | ACNGT | 9 cut(s) | 784, 1076, 1525, 1613, 2009, 2623, 2738, 3422, 3793 |
| Bst6I | CTCTTC | 2 cut(s) | 1795, 3471 |
| BstBAI | YACGTR | 1 cut(s) | 1940 |
| BstBI | TTCGAA | 2 cut(s) | 421, 1600 |
| BstC8I | GCNNGC | 8 cut(s) | 170, 190, 1632, 2584, 2649, 2861, 3374, 4008 |
| BstDSI | CCRYGG | 1 cut(s) | 1737 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 3980 |
| BstFNI | CGCG | 4 cut(s) | 648, 755, 1992, 2447 |
| BstHHI | GCGC | 3 cut(s) | 958, 1808, 1994 |
| BstMCI | CGRYCG | 1 cut(s) | 3795 |
| BstMWI | GCNNNNNNNGC | 8 cut(s) | 231, 627, 1844, 1853, 2728, 3882, 3965, 4012 |
| BstNI | CCWGG | 8 cut(s) | 803, 1314, 2164, 2566, 2888, 3170, 3546, 3753 |
| BstNSI | RCATGY | 4 cut(s) | 120, 192, 261, 3376 |
| BstSFI | CTRYAG | 3 cut(s) | 687, 2790, 3915 |
| BstSLI | GKGCMC | 2 cut(s) | 533, 3268 |
| BstUI | CGCG | 4 cut(s) | 648, 755, 1992, 2447 |
| BstV2I | GAAGAC | 6 cut(s) | 1454, 1910, 3032, 3216, 3350, 3788 |
| BstX2I | RGATCY | 3 cut(s) | 3308, 3325, 3601 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 3755 |
| BstYI | RGATCY | 3 cut(s) | 3308, 3325, 3601 |
| Bsu15I | ATCGAT | 1 cut(s) | 144 |
| Bsu36I | CCTNAGG | 1 cut(s) | 525 |
| BsuRI | GGCC | 3 cut(s) | 630, 856, 3133 |
| BsuTUI | ATCGAT | 1 cut(s) | 144 |
| BtgI | CCRYGG | 1 cut(s) | 1737 |
| BtgZI | GCGATG | 2 cut(s) | 736, 2015 |
| BtsI | GCAGTG | 2 cut(s) | 1360, 1905 |
| BtuMI | TCGCGA | 1 cut(s) | 648 |
| Cac8I | GCNNGC | 8 cut(s) | 170, 190, 1632, 2584, 2649, 2861, 3374, 4008 |
| CaiI | CAGNNNCTG | 1 cut(s) | 1753 |
| CciI | TCATGA | 1 cut(s) | 1704 |
| CfoI | GCGC | 3 cut(s) | 958, 1808, 1994 |
| Cfr10I | RCCGGY | 1 cut(s) | 3035 |
| Cfr13I | GGNCC | 9 cut(s) | 629, 799, 855, 1859, 1999, 2005, 3104, 3131, 3512 |
| Cfr9I | CCCGGG | 1 cut(s) | 860 |
| ClaI | ATCGAT | 1 cut(s) | 144 |
| CseI | GACGC | 1 cut(s) | 23 |
| CsiI | ACCWGGT | 1 cut(s) | 3751 |
| CspCI | CAANNNNNGTGG | 6 cut(s) | 6, 41, 2257, 2292, 3890, 3925 |
| DrdI | GACNNNNNNGTC | 2 cut(s) | 1417, 3433 |
| DriI | GACNNNNNGTC | 1 cut(s) | 805 |
| DseDI | GACNNNNNNGTC | 2 cut(s) | 1417, 3433 |
| Eam1104I | CTCTTC | 2 cut(s) | 1795, 3471 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 805 |
| EarI | CTCTTC | 2 cut(s) | 1795, 3471 |
| Ecl136II | GAGCTC | 1 cut(s) | 896 |
| Eco130I | CCWWGG | 2 cut(s) | 1623, 1737 |
| Eco24I | GRGCYC | 3 cut(s) | 898, 1118, 2932 |
| Eco31I | GGTCTC | 2 cut(s) | 1403, 2665 |
| Eco32I | GATATC | 1 cut(s) | 3118 |
| Eco47I | GGWCC | 6 cut(s) | 799, 1859, 1999, 2005, 3104, 3512 |
| Eco53kI | GAGCTC | 1 cut(s) | 896 |
| Eco57I | CTGAAG | 8 cut(s) | 1176, 1239, 1251, 2451, 2538, 2940, 3281, 3369 |
| Eco81I | CCTNAGG | 1 cut(s) | 525 |
| Eco88I | CYCGRG | 1 cut(s) | 860 |
| EcoICRI | GAGCTC | 1 cut(s) | 896 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 3980 |
| EcoO109I | RGGNCCY | 4 cut(s) | 799, 855, 1859, 3131 |
| EcoRII | CCWGG | 8 cut(s) | 801, 1312, 2162, 2564, 2886, 3168, 3544, 3751 |
| EcoRV | GATATC | 1 cut(s) | 3118 |
| EcoT14I | CCWWGG | 2 cut(s) | 1623, 1737 |
| EcoT22I | ATGCAT | 2 cut(s) | 131, 194 |
| EcoT38I | GRGCYC | 3 cut(s) | 898, 1118, 2932 |
| ErhI | CCWWGG | 2 cut(s) | 1623, 1737 |
| Esp3I | CGTCTC | 4 cut(s) | 6, 309, 704, 2301 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 2204, 2236, 2754, 2786 |
| FaqI | GGGAC | 5 cut(s) | 1981, 2012, 2018, 3090, 3439 |
| FauI | CCCGC | 2 cut(s) | 2118, 2579 |
| FbaI | TGATCA | 1 cut(s) | 252 |
| FriOI | GRGCYC | 3 cut(s) | 898, 1118, 2932 |
| FspBI | CTAG | 7 cut(s) | 905, 2366, 2421, 2648, 2864, 2922, 3323 |
| GlaI | GCGC | 3 cut(s) | 957, 1807, 1993 |
| GsaI | CCCAGC | 1 cut(s) | 883 |
| GsuI | CTGGAG | 1 cut(s) | 824 |
| HaeIII | GGCC | 3 cut(s) | 630, 856, 3133 |
| HapII | CCGG | 4 cut(s) | 861, 1029, 2002, 3036 |
| HgaI | GACGC | 1 cut(s) | 23 |
| HhaI | GCGC | 3 cut(s) | 958, 1808, 1994 |
| Hin6I | GCGC | 3 cut(s) | 956, 1806, 1992 |
| HinP1I | GCGC | 3 cut(s) | 956, 1806, 1992 |
| HincII | GTYRAC | 2 cut(s) | 1135, 1548 |
| HindII | GTYRAC | 2 cut(s) | 1135, 1548 |
| HindIII | AAGCTT | 1 cut(s) | 1826 |
| HpaI | GTTAAC | 1 cut(s) | 1548 |
| HpaII | CCGG | 4 cut(s) | 861, 1029, 2002, 3036 |
| HphI | GGTGA | 7 cut(s) | 297, 719, 1628, 3485, 3737, 4001, 4009 |
| Hpy166II | GTNNAC | 5 cut(s) | 295, 1135, 1548, 2145, 3068 |
| Hpy8I | GTNNAC | 5 cut(s) | 295, 1135, 1548, 2145, 3068 |
| Hpy99I | CGWCG | 1 cut(s) | 323 |
| HpyCH4III | ACNGT | 9 cut(s) | 784, 1076, 1525, 1613, 2009, 2623, 2738, 3422, 3793 |
| HpyCH4IV | ACGT | 4 cut(s) | 1362, 1544, 1939, 4020 |
| HpyF10VI | GCNNNNNNNGC | 8 cut(s) | 231, 627, 1844, 1853, 2728, 3882, 3965, 4012 |
| HpySE526I | ACGT | 4 cut(s) | 1362, 1544, 1939, 4020 |
| HspAI | GCGC | 3 cut(s) | 956, 1806, 1992 |
| Kpn2I | TCCGGA | 1 cut(s) | 1028 |
| KpnI | GGTACC | 1 cut(s) | 499 |
| Ksp22I | TGATCA | 1 cut(s) | 252 |
| KspAI | GTTAAC | 1 cut(s) | 1548 |
| LguI | GCTCTTC | 1 cut(s) | 3471 |
| LmnI | GCTCC | 8 cut(s) | 285, 757, 893, 1315, 1870, 2927, 3448, 3548 |
| MabI | ACCWGGT | 1 cut(s) | 3751 |
| MaeI | CTAG | 7 cut(s) | 905, 2366, 2421, 2648, 2864, 2922, 3323 |
| MaeII | ACGT | 4 cut(s) | 1362, 1544, 1939, 4020 |
| MaeIII | GTNAC | 6 cut(s) | 34, 70, 485, 1058, 1781, 2531 |
| MbiI | CCGCTC | 1 cut(s) | 2125 |
| MfeI | CAATTG | 4 cut(s) | 199, 248, 468, 1458 |
| MflI | RGATCY | 3 cut(s) | 3308, 3325, 3601 |
| MhlI | GDGCHC | 9 cut(s) | 533, 898, 1118, 2628, 2751, 2932, 3268, 3445, 3861 |
| MlyI | GAGTC | 8 cut(s) | 296, 815, 1427, 1444, 1493, 1724, 2138, 3443 |
| MmeI | TCCRAC | 1 cut(s) | 715 |
| Mph1103I | ATGCAT | 2 cut(s) | 131, 194 |
| MroI | TCCGGA | 1 cut(s) | 1028 |
| MslI | CAYNNNNRTG | 1 cut(s) | 550 |
| MspA1I | CMGCKG | 1 cut(s) | 2471 |
| MspI | CCGG | 4 cut(s) | 861, 1029, 2002, 3036 |
| MunI | CAATTG | 4 cut(s) | 199, 248, 468, 1458 |
| MvaI | CCWGG | 8 cut(s) | 803, 1314, 2164, 2566, 2888, 3170, 3546, 3753 |
| MvnI | CGCG | 4 cut(s) | 648, 755, 1992, 2447 |
| MwoI | GCNNNNNNNGC | 8 cut(s) | 231, 627, 1844, 1853, 2728, 3882, 3965, 4012 |
| NciI | CCSGG | 3 cut(s) | 861, 862, 2003 |
| NcoI | CCATGG | 1 cut(s) | 1737 |
| NheI | GCTAGC | 1 cut(s) | 2647 |
| NmuCI | GTSAC | 2 cut(s) | 485, 2531 |
| NruI | TCGCGA | 1 cut(s) | 648 |
| NsiI | ATGCAT | 2 cut(s) | 131, 194 |
| NspI | RCATGY | 4 cut(s) | 120, 192, 261, 3376 |
| NspV | TTCGAA | 2 cut(s) | 421, 1600 |
| PaeI | GCATGC | 2 cut(s) | 192, 3376 |
| PagI | TCATGA | 1 cut(s) | 1704 |
| PciI | ACATGT | 2 cut(s) | 116, 257 |
| PciSI | GCTCTTC | 1 cut(s) | 3471 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 645 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 847, 890 |
| PfoI | TCCNGGA | 2 cut(s) | 1312, 3544 |
| PleI | GAGTC | 8 cut(s) | 296, 814, 1426, 1444, 1493, 1724, 2137, 3442 |
| PpsI | GAGTC | 8 cut(s) | 296, 814, 1426, 1444, 1493, 1724, 2137, 3442 |
| Ppu21I | YACGTR | 1 cut(s) | 1940 |
| PpuMI | RGGWCCY | 2 cut(s) | 799, 1859 |
| PscI | ACATGT | 2 cut(s) | 116, 257 |
| PshBI | ATTAAT | 1 cut(s) | 2823 |
| Psp124BI | GAGCTC | 1 cut(s) | 898 |
| Psp5II | RGGWCCY | 2 cut(s) | 799, 1859 |
| Psp6I | CCWGG | 8 cut(s) | 801, 1312, 2162, 2564, 2886, 3168, 3544, 3751 |
| PspFI | CCCAGC | 1 cut(s) | 879 |
| PspGI | CCWGG | 8 cut(s) | 801, 1312, 2162, 2564, 2886, 3168, 3544, 3751 |
| PspPI | GGNCC | 9 cut(s) | 629, 799, 855, 1859, 1999, 2005, 3104, 3131, 3512 |
| PspPPI | RGGWCCY | 2 cut(s) | 799, 1859 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 2779, 2811 |
| PstI | CTGCAG | 2 cut(s) | 2794, 3919 |
| PstNI | CAGNNNCTG | 1 cut(s) | 1753 |
| PsuI | RGATCY | 3 cut(s) | 3308, 3325, 3601 |
| PvuII | CAGCTG | 1 cut(s) | 2471 |
| RruI | TCGCGA | 1 cut(s) | 648 |
| RseI | CAYNNNNRTG | 1 cut(s) | 550 |
| SacI | GAGCTC | 1 cut(s) | 898 |
| SapI | GCTCTTC | 1 cut(s) | 3471 |
| Sau96I | GGNCC | 9 cut(s) | 629, 799, 855, 1859, 1999, 2005, 3104, 3131, 3512 |
| ScaI | AGTACT | 2 cut(s) | 976, 2937 |
| SchI | GAGTC | 8 cut(s) | 296, 815, 1427, 1444, 1493, 1724, 2138, 3443 |
| SduI | GDGCHC | 9 cut(s) | 533, 898, 1118, 2628, 2751, 2932, 3268, 3445, 3861 |
| SexAI | ACCWGGT | 1 cut(s) | 3751 |
| SfcI | CTRYAG | 3 cut(s) | 687, 2790, 3915 |
| SfuI | TTCGAA | 2 cut(s) | 421, 1600 |
| SinI | GGWCC | 6 cut(s) | 799, 1859, 1999, 2005, 3104, 3512 |
| SmaI | CCCGGG | 1 cut(s) | 862 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 550 |
| SmlI | CTYRAG | 2 cut(s) | 2755, 3377 |
| SmoI | CTYRAG | 2 cut(s) | 2755, 3377 |
| SphI | GCATGC | 2 cut(s) | 192, 3376 |
| SsiI | CCGC | 5 cut(s) | 755, 2125, 2447, 2586, 3869 |
| SspI | AATATT | 4 cut(s) | 670, 1190, 1265, 2392 |
| SspMI | CTAG | 7 cut(s) | 905, 2366, 2421, 2648, 2864, 2922, 3323 |
| SstI | GAGCTC | 1 cut(s) | 898 |
| StyI | CCWWGG | 2 cut(s) | 1623, 1737 |
| TaaI | ACNGT | 9 cut(s) | 784, 1076, 1525, 1613, 2009, 2623, 2738, 3422, 3793 |
| TaiI | ACGT | 4 cut(s) | 1365, 1547, 1942, 4023 |
| TaqI | TCGA | 4 cut(s) | 144, 421, 1041, 1600 |
| TatI | WGTACW | 7 cut(s) | 542, 604, 974, 2309, 2428, 2935, 3352 |
| TseFI | GTSAC | 2 cut(s) | 485, 2531 |
| Tsp45I | GTSAC | 2 cut(s) | 485, 2531 |
| TspGWI | ACGGA | 3 cut(s) | 1883, 2771, 2813 |
| TspMI | CCCGGG | 1 cut(s) | 860 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 847, 890 |
| VpaK11BI | GGWCC | 6 cut(s) | 799, 1859, 1999, 2005, 3104, 3512 |
| VspI | ATTAAT | 1 cut(s) | 2823 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 3980 |
| XapI | RAATTY | 7 cut(s) | 242, 417, 946, 1959, 2018, 2317, 2677 |
| XbaI | TCTAGA | 1 cut(s) | 3322 |
| XceI | RCATGY | 4 cut(s) | 120, 192, 261, 3376 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 887, 2500 |
| XmaI | CCCGGG | 1 cut(s) | 860 |
| XspI | CTAG | 7 cut(s) | 905, 2366, 2421, 2648, 2864, 2922, 3323 |
| ZrmI | AGTACT | 2 cut(s) | 976, 2937 |
| Zsp2I | ATGCAT | 2 cut(s) | 131, 194 |