Prupe.5G197400_v2.0.a1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0016849
Physical Location & Seq
Forward (+)
15960862 .. 15963047
2186 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G197400.1

Sequence Viewer

Length: 1671 bp
ATGTCGGTTATGGAAGCCATTCCCGCCAACAACCCTGCAACCTCTAGAGCTCTACAGCAGTACCTCTTCTCTCTATTACAGAGCTGCAACTCCATAAGAAAACTTACCCAAATTCATACCCAAATACTTGTTCACGGGTTCAACCAAAAAAGCTACATTCTTGTTAGATTATTATCATTCTATGTATCTTCTGGGTGTCTCCATCATGCCCTCAAAATTTTTGAAAATGTCGAAAACCCAAGTACTACTTTGTGGAACCAGATAATTAGAGGCCATTCCAGAAGTGAAACTCCCCGAAAATCTGTTCAACTATATAATCGGCTGGTGGGTGTGGGGGCTGAGCCTGATGGGTTTACTTATTTGTATGTTCTTAGTGCTTGTGCGAGGTCAGGGTTGGTGAGAGAAGGGAAGCAGGTGCATGCGAGAGTTTTGGCAAATGGGTTTGGTTCTAATGTGTTTGTCCAGACGAGTTTGGTTAATTTGTATGCAATTTGTGGAGGGTCAGGGTGTGGCGTTGAGTATGCACGCCGGCTGTTTGACGATATGGGTGAGAGGAGTGTTGTGAGTTGGAATTCACTGCTTGCGGGGTATATAAGATGTCGGAATGTTGATGGGGCGCGGCAAGTTTTTGACGAGATGCCAGAGAGGAATATTGTCTCGTGGACAACCATGATTGCGGGGTGTGCTCAAAACGGGAGGTGCAAGCAAGCTTTGTCTTTGTTTGGTCAGATGAGGAGGTCCAATGTGGAACTGGACCAGGTGGCGCTAGTGGCAGCGTTATCGGCCTGTGCTGAAATAGGGGACTTGAAATTGGGAAGGTGGATTCACTGGTACATTGAAGAGAGATTGTGGATGAAGTCCGAGCCACGGTTGGTGACTTTGTACAATGCACTCATACACATGTATGCCAGTTGCGGTTTAATTGAAGAAGCCTATAAATTGTTCAATCAGATGCCAAGAAGAAGCACTGTCTCGTGGACCAGCATCATCGTTGGGTTTGCAAAGCAGGGTCACGGGGAGGAAGCCCTTCATATCTTTCAATTGATGCTGAGCTCGGGAGTTGATGATGTAAGACCTGATGAGATAACTTTCGTTGGTGTCTTGTGTGCTTGTAGCCATGCTGGATTAGTTGACGAGGGCCGCCGAATTTTCAAGTTCATGATGCAAACTTGCGGACTTACCCCAAGGATTGAGCACTATGGGTGCATGGTTGATCTCTTGAGCCGAGCCGGTTTTCTAGAAGAAGCACATGTGCTTGTGGAGACTATGCCCATGAATCCGAACGAAGCTGTGTGGGGTGCTCTCCTTGGCGGTTGTAGGCTTCACAAGAATGCTGAGCTCGCATCACATGTGGCTAAAACATTGACAGTTGCACTTGACCCTGACCAAGCTGCAGGTTATCTTGTGCTCTTATCAAACGTGTATGCCTCTACTAAAAGATGGCTAGATGTTGCTTCTGTGAGGCAGAAAATGGTTAAGATGGGCGTGAGAAAGCCCCCAGGCCGAAGTTGGGTTCAAATCAATGGAGTTGTTCATGATTTTGTGGCAGGTGATAGGACCCATAAGCATGCAACTGTAATCTATGAGATGCTTGGTAAAATCACCAGGCCAGCACTGCAGGAGGGCTCCAAACCAGACATATCAGATATGCTTTCCCATGTTGCAGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2

Protein Analysis

557

Amino Acids

61.9

Weight (kDa)

8.72

Isoelectric Point (pI)

46.06

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016849)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g14600
malus_domestica MD06G1197100.v1.1
prunus_persica Prupe.5G197400_v2.0.a1
pyrus_communis pycom06g17390
rosa_chinensis RchiOBHm_Chr7g0181891
rosa_laevigata RLG00000005165
rosa_multiflora Rmu_co8431917.1_g000001 Rmu_sc0021327.1_g000007
rosa_roxburghii Rroxscaffold_3G00271660
rosa_rugosa Rorug06G0444800
rosa_samantha Rh7AG047100 Rh7BG046700 Rh7CG047200
rosa_wichuraiana Rw7G003900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 403, 1538
Acc36I ACCTGC 3 cut(s) 403, 1385, 1538
AccII CGCG 1 cut(s) 619
AciI CCGC 8 cut(s) 24, 584, 619, 677, 915, 1141, 1173, 1311
AcsI RAATTY 4 cut(s) 111, 216, 571, 1146
AfaI GTAC 4 cut(s) 62, 244, 833, 884
AfiI CCNNNNNNNGG 2 cut(s) 867, 1510
AflIII ACRYGT 4 cut(s) 900, 1249, 1348, 1419
AjnI CCWGG 3 cut(s) 756, 1498, 1604
AjuI GAANNNNNNNTTGG 2 cut(s) 114, 146
AluBI AGCT 8 cut(s) 50, 84, 153, 710, 1053, 1289, 1339, 1391
AluI AGCT 8 cut(s) 50, 84, 153, 710, 1053, 1289, 1339, 1391
Alw21I GWGCWC 7 cut(s) 52, 688, 1055, 1197, 1303, 1341, 1410
Alw26I GTCTC 4 cut(s) 203, 661, 976, 1256
Ama87I CYCGRG 1 cut(s) 1054
AoxI GGCC 5 cut(s) 271, 783, 1138, 1501, 1607
ApeKI GCWGC 3 cut(s) 84, 773, 1391
ApoI RAATTY 4 cut(s) 111, 216, 571, 1146
Asp700I GAANNNNTTC 2 cut(s) 18, 1026
AspLEI GCGC 2 cut(s) 619, 766
AspS9I GGNCC 5 cut(s) 738, 754, 978, 1138, 1557
AsuHPI GGTGA 5 cut(s) 409, 560, 886, 1562, 1594
AvaI CYCGRG 1 cut(s) 1054
AvaII GGWCC 4 cut(s) 738, 754, 978, 1557
BaeI ACNNNNGTAYC 2 cut(s) 44, 77
BanII GRGCYC 4 cut(s) 52, 1055, 1341, 1628
BauI CACGAG 2 cut(s) 658, 973
Bbv12I GWGCWC 7 cut(s) 52, 688, 1055, 1197, 1303, 1341, 1410
BbvI GCAGC 3 cut(s) 71, 785, 1378
BccI CCATC 5 cut(s) 210, 341, 605, 1434, 1474
BcgI CGANNNNNNTGC 2 cut(s) 762, 796
BciT130I CCWGG 3 cut(s) 758, 1500, 1606
BcoDI GTCTC 4 cut(s) 203, 661, 976, 1256
BfaI CTAG 4 cut(s) 45, 767, 1238, 1445
BfmI CTRYAG 3 cut(s) 53, 1392, 1616
BfoI RGCGCY 1 cut(s) 767
BfuAI ACCTGC 3 cut(s) 403, 1385, 1538
BisI GCNGC 5 cut(s) 85, 620, 774, 1141, 1392
BlpI GCTNAGC 3 cut(s) 339, 1049, 1335
BlsI GCNGC 5 cut(s) 86, 621, 775, 1142, 1393
BmcAI AGTACT 1 cut(s) 244
Bme1390I CCNGG 3 cut(s) 758, 1500, 1606
Bme18I GGWCC 4 cut(s) 738, 754, 978, 1557
BmeT110I CYCGRG 1 cut(s) 1054
BmgT120I GGNCC 5 cut(s) 738, 754, 978, 1138, 1557
BmiI GGNNCC 3 cut(s) 257, 1559, 1627
BmrFI CCNGG 3 cut(s) 758, 1500, 1606
BmsI GCATC 7 cut(s) 627, 942, 993, 1035, 1152, 1352, 1578
Bpu1102I GCTNAGC 3 cut(s) 339, 1049, 1335
BpuEI CTTGAG 1 cut(s) 1240
BsaBI GATNNNNATC 1 cut(s) 172
BsaJI CCNNGG 4 cut(s) 866, 1184, 1306, 1498
Bsc4I CCNNNNNNNGG 2 cut(s) 867, 1510
Bse118I RCCGGY 2 cut(s) 528, 1229
Bse1I ACTGG 3 cut(s) 756, 833, 909
Bse8I GATNNNNATC 1 cut(s) 172
BseBI CCWGG 3 cut(s) 758, 1500, 1606
BseDI CCNNGG 4 cut(s) 866, 1184, 1306, 1498
BseGI GGATG 1 cut(s) 858
BseJI GATNNNNATC 1 cut(s) 172
BseLI CCNNNNNNNGG 2 cut(s) 867, 1510
BseMII CTCAG 3 cut(s) 330, 1040, 1326
BseNI ACTGG 3 cut(s) 756, 833, 909
BseRI GAGGAG 2 cut(s) 568, 748
BseXI GCAGC 3 cut(s) 71, 785, 1378
Bsh1236I CGCG 1 cut(s) 619
BshFI GGCC 5 cut(s) 273, 785, 1140, 1503, 1609
BsiHKAI GWGCWC 7 cut(s) 52, 688, 1055, 1197, 1303, 1341, 1410
BsiHKCI CYCGRG 1 cut(s) 1054
BsiSI CCGG 2 cut(s) 529, 1230
BslFI GGGAC 1 cut(s) 815
BslI CCNNNNNNNGG 2 cut(s) 867, 1510
BsmAI GTCTC 4 cut(s) 203, 661, 976, 1256
BsmFI GGGAC 1 cut(s) 815
BsmI GAATGC 1 cut(s) 1336
BsnI GGCC 5 cut(s) 273, 785, 1140, 1503, 1609
BsoBI CYCGRG 1 cut(s) 1054
Bsp1286I GDGCHC 8 cut(s) 52, 688, 1055, 1197, 1303, 1341, 1410, 1628
Bsp1407I TGTACA 1 cut(s) 882
Bsp143I GATC 1 cut(s) 1213
Bsp1720I GCTNAGC 3 cut(s) 339, 1049, 1335
BspACI CCGC 8 cut(s) 24, 584, 619, 677, 915, 1141, 1173, 1311
BspANI GGCC 5 cut(s) 273, 785, 1140, 1503, 1609
BspCNI CTCAG 3 cut(s) 331, 1041, 1327
BspFNI CGCG 1 cut(s) 619
BspHI TCATGA 2 cut(s) 1158, 1534
BspLI GGNNCC 3 cut(s) 257, 1559, 1627
BspMAI CTGCAG 2 cut(s) 1396, 1620
BspMI ACCTGC 3 cut(s) 403, 1385, 1538
BsrFI RCCGGY 2 cut(s) 528, 1229
BsrGI TGTACA 1 cut(s) 882
BsrI ACTGG 3 cut(s) 756, 833, 909
BssAI RCCGGY 2 cut(s) 528, 1229
BssECI CCNNGG 4 cut(s) 866, 1184, 1306, 1498
BssMI GATC 1 cut(s) 1213
BssSI CACGAG 2 cut(s) 658, 973
BssT1I CCWWGG 2 cut(s) 1184, 1306
Bst2BI CACGAG 2 cut(s) 658, 973
Bst2UI CCWGG 3 cut(s) 758, 1500, 1606
Bst4CI ACNGT 4 cut(s) 870, 970, 1369, 1576
Bst6I CTCTTC 2 cut(s) 71, 834
BstAUI TGTACA 1 cut(s) 882
BstC8I GCNNGC 9 cut(s) 420, 526, 530, 582, 704, 708, 1341, 1569, 1611
BstDEI CTNAG 4 cut(s) 339, 371, 1049, 1335
BstDSI CCRYGG 1 cut(s) 866
BstF5I GGATG 1 cut(s) 858
BstFNI CGCG 1 cut(s) 619
BstH2I RGCGCY 1 cut(s) 767
BstHHI GCGC 2 cut(s) 619, 766
BstKTI GATC 1 cut(s) 1216
BstMAI GTCTC 4 cut(s) 203, 661, 976, 1256
BstMBI GATC 1 cut(s) 1213
BstMWI GCNNNNNNNGC 6 cut(s) 23, 683, 770, 782, 1340, 1615
BstNI CCWGG 3 cut(s) 758, 1500, 1606
BstNSI RCATGY 5 cut(s) 422, 904, 1253, 1352, 1571
BstSCI CCNGG 3 cut(s) 756, 1498, 1604
BstSFI CTRYAG 3 cut(s) 53, 1392, 1616
BstUI CGCG 1 cut(s) 619
BstV1I GCAGC 3 cut(s) 71, 785, 1378
BsuRI GGCC 5 cut(s) 273, 785, 1140, 1503, 1609
BtgI CCRYGG 1 cut(s) 866
BtsCI GGATG 1 cut(s) 858
BtsI GCAGTG 2 cut(s) 575, 1613
BtsIMutI CAGTG 4 cut(s) 575, 826, 966, 1613
BveI ACCTGC 3 cut(s) 403, 1385, 1538
Cac8I GCNNGC 9 cut(s) 420, 526, 530, 582, 704, 708, 1341, 1569, 1611
CciI TCATGA 2 cut(s) 1158, 1534
CfoI GCGC 2 cut(s) 619, 766
Cfr10I RCCGGY 2 cut(s) 528, 1229
Cfr13I GGNCC 5 cut(s) 738, 754, 978, 1138, 1557
CsiI ACCWGGT 1 cut(s) 756
Csp6I GTAC 4 cut(s) 61, 243, 832, 883
CviQI GTAC 4 cut(s) 61, 243, 832, 883
DdeI CTNAG 4 cut(s) 339, 371, 1049, 1335
DpnI GATC 1 cut(s) 1215
DpnII GATC 1 cut(s) 1213
Eam1104I CTCTTC 2 cut(s) 71, 834
EarI CTCTTC 2 cut(s) 71, 834
Ecl136II GAGCTC 3 cut(s) 50, 1053, 1339
Eco130I CCWWGG 2 cut(s) 1184, 1306
Eco24I GRGCYC 4 cut(s) 52, 1055, 1341, 1628
Eco47I GGWCC 4 cut(s) 738, 754, 978, 1557
Eco53kI GAGCTC 3 cut(s) 50, 1053, 1339
Eco88I CYCGRG 1 cut(s) 1054
EcoICRI GAGCTC 3 cut(s) 50, 1053, 1339
EcoO109I RGGNCCY 1 cut(s) 1557
EcoRI GAATTC 1 cut(s) 571
EcoRII CCWGG 3 cut(s) 756, 1498, 1604
EcoT14I CCWWGG 2 cut(s) 1184, 1306
EcoT38I GRGCYC 4 cut(s) 52, 1055, 1341, 1628
ErhI CCWWGG 2 cut(s) 1184, 1306
FalI AAGNNNNNCTT 2 cut(s) 232, 264
FaqI GGGAC 1 cut(s) 815
FauI CCCGC 3 cut(s) 31, 577, 670
Fnu4HI GCNGC 5 cut(s) 85, 620, 774, 1141, 1392
FokI GGATG 1 cut(s) 865
FriOI GRGCYC 4 cut(s) 52, 1055, 1341, 1628
Fsp4HI GCNGC 5 cut(s) 85, 620, 774, 1141, 1392
FspBI CTAG 4 cut(s) 45, 767, 1238, 1445
GlaI GCGC 2 cut(s) 618, 765
GluI GCNGC 5 cut(s) 85, 620, 774, 1141, 1392
HaeII RGCGCY 1 cut(s) 767
HaeIII GGCC 5 cut(s) 273, 785, 1140, 1503, 1609
HapII CCGG 2 cut(s) 529, 1230
HhaI GCGC 2 cut(s) 619, 766
Hin6I GCGC 2 cut(s) 617, 764
HinP1I GCGC 2 cut(s) 617, 764
HincII GTYRAC 1 cut(s) 1132
HindII GTYRAC 1 cut(s) 1132
HindIII AAGCTT 1 cut(s) 708
HinfI GANTC 2 cut(s) 823, 1276
HpaII CCGG 2 cut(s) 529, 1230
HphI GGTGA 5 cut(s) 409, 560, 886, 1562, 1594
Hpy166II GTNNAC 5 cut(s) 133, 354, 663, 978, 1132
Hpy188I TCNGA 6 cut(s) 603, 729, 862, 951, 1281, 1645
Hpy188III TCNNGA 8 cut(s) 45, 279, 463, 1056, 1159, 1219, 1238, 1535
Hpy8I GTNNAC 5 cut(s) 133, 354, 663, 978, 1132
HpyAV CCTTC 3 cut(s) 398, 810, 1037
HpyCH4III ACNGT 4 cut(s) 870, 970, 1369, 1576
HpyCH4IV ACGT 1 cut(s) 1419
HpyF10VI GCNNNNNNNGC 6 cut(s) 23, 683, 770, 782, 1340, 1615
HpyF3I CTNAG 4 cut(s) 339, 371, 1049, 1335
HpySE526I ACGT 1 cut(s) 1419
HspAI GCGC 2 cut(s) 617, 764
KroI GCCGGC 1 cut(s) 528
KroNI GCCGGC 1 cut(s) 530
Kzo9I GATC 1 cut(s) 1213
LmnI GCTCC 1 cut(s) 1631
Lsp1109I GCAGC 3 cut(s) 71, 785, 1378
LweI GCATC 7 cut(s) 627, 942, 993, 1035, 1152, 1352, 1578
MabI ACCWGGT 1 cut(s) 756
MaeI CTAG 4 cut(s) 45, 767, 1238, 1445
MaeII ACGT 1 cut(s) 1419
MaeIII GTNAC 2 cut(s) 874, 1010
MalI GATC 1 cut(s) 1215
MboI GATC 1 cut(s) 1213
MboII GAAGA 6 cut(s) 58, 180, 851, 938, 972, 1253
MfeI CAATTG 1 cut(s) 1040
MhlI GDGCHC 8 cut(s) 52, 688, 1055, 1197, 1303, 1341, 1410, 1628
MmeI TCCRAC 2 cut(s) 548, 581
MroNI GCCGGC 1 cut(s) 528
MroXI GAANNNNTTC 2 cut(s) 18, 1026
MseI TTAA 3 cut(s) 477, 920, 1476
MslI CAYNNNNRTG 4 cut(s) 899, 903, 1329, 1566
MspI CCGG 2 cut(s) 529, 1230
MspR9I CCNGG 3 cut(s) 758, 1500, 1606
MunI CAATTG 1 cut(s) 1040
Mva1269I GAATGC 1 cut(s) 1336
MvaI CCWGG 3 cut(s) 758, 1500, 1606
MvnI CGCG 1 cut(s) 619
MwoI GCNNNNNNNGC 6 cut(s) 23, 683, 770, 782, 1340, 1615
NaeI GCCGGC 1 cut(s) 530
NdeII GATC 1 cut(s) 1213
NgoMIV GCCGGC 1 cut(s) 528
NlaIV GGNNCC 3 cut(s) 257, 1559, 1627
NmeAIII GCCGAG 1 cut(s) 1250
NmuCI GTSAC 2 cut(s) 874, 1010
NspI RCATGY 5 cut(s) 422, 904, 1253, 1352, 1571
PaeI GCATGC 2 cut(s) 422, 1571
PagI TCATGA 2 cut(s) 1158, 1534
PaqCI CACCTGC 2 cut(s) 403, 1538
PciI ACATGT 3 cut(s) 900, 1249, 1348
PctI GAATGC 1 cut(s) 1336
PdiI GCCGGC 1 cut(s) 530
PdmI GAANNNNTTC 2 cut(s) 18, 1026
PfeI GAWTC 2 cut(s) 823, 1276
PkrI GCNGC 5 cut(s) 86, 621, 775, 1142, 1393
PpuMI RGGWCCY 1 cut(s) 1557
PscI ACATGT 3 cut(s) 900, 1249, 1348
Psp124BI GAGCTC 3 cut(s) 52, 1055, 1341
Psp5II RGGWCCY 1 cut(s) 1557
Psp6I CCWGG 3 cut(s) 756, 1498, 1604
PspGI CCWGG 3 cut(s) 756, 1498, 1604
PspN4I GGNNCC 3 cut(s) 257, 1559, 1627
PspPI GGNCC 5 cut(s) 738, 754, 978, 1138, 1557
PspPPI RGGWCCY 1 cut(s) 1557
PstI CTGCAG 2 cut(s) 1396, 1620
RsaI GTAC 4 cut(s) 62, 244, 833, 884
RsaNI GTAC 4 cut(s) 61, 243, 832, 883
RseI CAYNNNNRTG 4 cut(s) 899, 903, 1329, 1566
SacI GAGCTC 3 cut(s) 52, 1055, 1341
SaqAI TTAA 3 cut(s) 477, 920, 1476
SatI GCNGC 5 cut(s) 85, 620, 774, 1141, 1392
Sau3AI GATC 1 cut(s) 1213
Sau96I GGNCC 5 cut(s) 738, 754, 978, 1138, 1557
ScaI AGTACT 1 cut(s) 244
ScrFI CCNGG 3 cut(s) 758, 1500, 1606
SduI GDGCHC 8 cut(s) 52, 688, 1055, 1197, 1303, 1341, 1410, 1628
SexAI ACCWGGT 1 cut(s) 756
SfaNI GCATC 7 cut(s) 627, 942, 993, 1035, 1152, 1352, 1578
SfcI CTRYAG 3 cut(s) 53, 1392, 1616
SinI GGWCC 4 cut(s) 738, 754, 978, 1557
SmiMI CAYNNNNRTG 4 cut(s) 899, 903, 1329, 1566
SmlI CTYRAG 1 cut(s) 1219
SmoI CTYRAG 1 cut(s) 1219
SphI GCATGC 2 cut(s) 422, 1571
SsiI CCGC 8 cut(s) 24, 584, 619, 677, 915, 1141, 1173, 1311
SspI AATATT 1 cut(s) 652
SspMI CTAG 4 cut(s) 45, 767, 1238, 1445
SstI GAGCTC 3 cut(s) 52, 1055, 1341
StyD4I CCNGG 3 cut(s) 756, 1498, 1604
StyI CCWWGG 2 cut(s) 1184, 1306
TaaI ACNGT 4 cut(s) 870, 970, 1369, 1576
TaiI ACGT 1 cut(s) 1422
TaqI TCGA 1 cut(s) 231
TatI WGTACW 2 cut(s) 242, 882
TauI GCSGC 2 cut(s) 622, 1143
TfiI GAWTC 2 cut(s) 823, 1276
Tru1I TTAA 3 cut(s) 477, 920, 1476
Tru9I TTAA 3 cut(s) 477, 920, 1476
TscAI CASTG 4 cut(s) 582, 833, 973, 1620
TseFI GTSAC 2 cut(s) 874, 1010
TseI GCWGC 3 cut(s) 84, 773, 1391
Tsp45I GTSAC 2 cut(s) 874, 1010
TspDTI ATGAA 6 cut(s) 104, 869, 1019, 1147, 1289, 1523
TspRI CASTG 4 cut(s) 582, 833, 973, 1620
VpaK11BI GGWCC 4 cut(s) 738, 754, 978, 1557
XapI RAATTY 4 cut(s) 111, 216, 571, 1146
XbaI TCTAGA 2 cut(s) 44, 1237
XceI RCATGY 5 cut(s) 422, 904, 1253, 1352, 1571
XcmI CCANNNNNNNNNTGG 2 cut(s) 748, 1506
XmnI GAANNNNTTC 2 cut(s) 18, 1026
XspI CTAG 4 cut(s) 45, 767, 1238, 1445
ZrmI AGTACT 1 cut(s) 244
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.