Rmu_sc0021327.1_g000007

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0021327.1
OG0016849
Physical Location & Seq
Forward (+)
23152 .. 24702
1551 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0021327.1_g000007.1.cds

Sequence Viewer

Length: 1551 bp
ctgacccaaattcatacacgggtagtagtccatggattcacccagaaaagctatattctttctaaggtactagcgttgtatgtaggttgtgggtgtcttgataatgcgctgagagttttcgggaatgtgaagagtccgagtactactgtgtggaaccagataatcagagggtatgctggcagcgaaactccggggaaggcggttgagttgtataacaggctggcggtgacggaagccgagccggatgggtatacgtattcgtatgttgttagtgcttgcgctaggtcagggatggttagagagggggagcagttgcatgggagagttttgggcggtgggtttggttctaatgtgtttgttcagaccagtttggttagtttgtatgcaggtggtggtgttgagtgcgcgcgcaaggtgtttgatgaaatgcgtgagaggagtgttgtcagttggaatacgctgctttcggggtatgtgaggagccgggattttgatggggcgcggcgagtttttgatgagatgccggagaggaatgtggtgtcgtggacgacaatgattgcggggtgtgctcagaatgggaggtgtaaggaagctttgagtttgtttgggcggatgaggaaggcggatgtggaaccggaccaggttgcgctagtggcggtgttatcggcctgtgctgaaattggggacttgagattgggaaggtgggttcactggtacgttgaagagggattgtgtgttagagctaagtcgcggttggtgactttgtacaatgcactcatacatatgtacgccagttgtggtttggttgatgaagcgtataaggtgttcaagcagatgcctagaaggagcactgtctcgtggacaagcatcatagttgggttcgcaaagcagggttgcggggaggaagctcttcgaatatttcaattgatgctgagctcggaacttgatgatgtaagacccgatgaaattactttccttggggtgttgtgtgcctgtagccatgctggattagttgatgagggcagaaggcttttcaagttcatgacgcagacttggggactcgaaccaaggattgagcactatggctgcatggtcgatctattaagccgtgctggttttttaaatgaagctcatgttcttgtggagactatgccaatggagccaaatgacgctgtgtggggtgctctcctcggcggttgcatgcttcacaagaattctgagcttgcatcacttgcagctcaaaatttaacagttgcacttgaccctgaccaagccgcagggtatcttgtgctcttatcgaatgtgtatgcttctgctaaaagatggctagatgttgcttatgtgagacagaaaatggttcacgtgggcgtgaagaagcctccaggtcgaagttgggttcaaatcaatggagctgttcatggttttgtggcaggtgatagaacccataaacatgaatctttgatctatgaaatggttggcgagatcacaaggcaagcccagcaagaaggccgcgaaccagacacattaaatacgctttcccttgaagactag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2

Protein Analysis

516

Amino Acids

57.08

Weight (kDa)

6.72

Isoelectric Point (pI)

39.51

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016849)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g14600
malus_domestica MD06G1197100.v1.1
prunus_persica Prupe.5G197400_v2.0.a1
pyrus_communis pycom06g17390
rosa_chinensis RchiOBHm_Chr7g0181891
rosa_laevigata RLG00000005165
rosa_multiflora Rmu_co8431917.1_g000001 Rmu_sc0021327.1_g000007
rosa_roxburghii Rroxscaffold_3G00271660
rosa_rugosa Rorug06G0444800
rosa_samantha Rh7AG047100 Rh7BG046700 Rh7CG047200
rosa_wichuraiana Rw7G003900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 377, 1421
Acc36I ACCTGC 2 cut(s) 377, 1421
AccI GTMKAC 1 cut(s) 251
AccII CGCG 5 cut(s) 407, 409, 502, 751, 1512
AcsI RAATTY 3 cut(s) 9, 1213, 1243
AcvI CACGTG 1 cut(s) 1363
AfaI GTAC 5 cut(s) 69, 142, 716, 767, 788
AgsI TTSAA 6 cut(s) 722, 829, 923, 1036, 1400, 1544
AjnI CCWGG 2 cut(s) 639, 1381
AloI GAACNNNNNNTCC 2 cut(s) 690, 722
AluBI AGCT 9 cut(s) 51, 593, 743, 908, 936, 1130, 1222, 1238, 1412
AluI AGCT 9 cut(s) 51, 593, 743, 908, 936, 1130, 1222, 1238, 1412
Alw21I GWGCWC 6 cut(s) 571, 851, 938, 1080, 1186, 1293
Alw26I GTCTC 3 cut(s) 859, 1139, 1339
AoxI GGCC 2 cut(s) 666, 1507
ApeKI GCWGC 4 cut(s) 180, 460, 1086, 1235
ApoI RAATTY 3 cut(s) 9, 1213, 1243
Asp700I GAANNNNTTC 1 cut(s) 909
AspLEI GCGC 7 cut(s) 109, 281, 407, 409, 411, 502, 649
AspS9I GGNCC 1 cut(s) 637
AsuC2I CCSGG 2 cut(s) 192, 485
AsuHPI GGTGA 4 cut(s) 31, 238, 769, 1445
AsuII TTCGAA 1 cut(s) 913
AvaII GGWCC 1 cut(s) 637
BaeI ACNNNNGTAYC 2 cut(s) 59, 92
BanII GRGCYC 1 cut(s) 938
BauI CACGAG 1 cut(s) 856
BbrPI CACGTG 1 cut(s) 1363
Bbv12I GWGCWC 6 cut(s) 571, 851, 938, 1080, 1186, 1293
BbvI GCAGC 4 cut(s) 192, 447, 1073, 1247
BccI CCATC 4 cut(s) 239, 286, 488, 1317
BceAI ACGGC 1 cut(s) 1092
BciT130I CCWGG 2 cut(s) 641, 1383
BcnI CCSGG 2 cut(s) 192, 485
BcoDI GTCTC 3 cut(s) 859, 1139, 1339
BfaI CTAG 6 cut(s) 71, 282, 650, 840, 1328, 1549
BfmI CTRYAG 1 cut(s) 994
BfuAI ACCTGC 2 cut(s) 377, 1421
BisI GCNGC 7 cut(s) 181, 461, 503, 1087, 1236, 1275, 1510
BlpI GCTNAGC 1 cut(s) 932
BlsI GCNGC 7 cut(s) 182, 462, 504, 1088, 1237, 1276, 1511
BmcAI AGTACT 1 cut(s) 142
Bme1390I CCNGG 4 cut(s) 192, 485, 641, 1383
Bme18I GGWCC 1 cut(s) 637
BmgT120I GGNCC 1 cut(s) 637
BmiI GGNNCC 4 cut(s) 155, 482, 633, 1161
BmrFI CCNGG 4 cut(s) 192, 485, 641, 1383
BmsI GCATC 5 cut(s) 510, 825, 876, 918, 1235
BpmI CTGGAG 1 cut(s) 1365
Bpu1102I GCTNAGC 1 cut(s) 932
Bpu14I TTCGAA 1 cut(s) 913
BpuEI CTTGAG 1 cut(s) 709
BpuMI CCSGG 2 cut(s) 192, 485
BsaAI YACGTR 2 cut(s) 255, 1363
BsaJI CCNNGG 5 cut(s) 31, 191, 976, 1067, 1189
BsaWI WCCGGW 1 cut(s) 634
Bse1I ACTGG 3 cut(s) 366, 716, 792
BseBI CCWGG 2 cut(s) 641, 1383
BseDI CCNNGG 5 cut(s) 31, 191, 976, 1067, 1189
BseGI GGATG 4 cut(s) 250, 297, 618, 631
BseMII CTCAG 4 cut(s) 101, 584, 923, 1209
BseNI ACTGG 3 cut(s) 366, 716, 792
BsePI GCGCGC 2 cut(s) 405, 407
BseRI GAGGAG 3 cut(s) 451, 493, 1178
BseXI GCAGC 4 cut(s) 192, 447, 1073, 1247
BseYI CCCAGC 1 cut(s) 1497
Bsh1236I CGCG 5 cut(s) 407, 409, 502, 751, 1512
BshFI GGCC 2 cut(s) 668, 1509
BsiHKAI GWGCWC 6 cut(s) 571, 851, 938, 1080, 1186, 1293
BsiSI CCGG 5 cut(s) 191, 242, 484, 524, 635
BslFI GGGAC 2 cut(s) 698, 1071
BsmAI GTCTC 3 cut(s) 859, 1139, 1339
BsmFI GGGAC 2 cut(s) 698, 1071
BsnI GGCC 2 cut(s) 668, 1509
Bsp119I TTCGAA 1 cut(s) 913
Bsp1286I GDGCHC 6 cut(s) 571, 851, 938, 1080, 1186, 1293
Bsp1407I TGTACA 1 cut(s) 765
Bsp143I GATC 3 cut(s) 1096, 1461, 1482
Bsp1720I GCTNAGC 1 cut(s) 932
Bsp19I CCATGG 1 cut(s) 31
BspANI GGCC 2 cut(s) 668, 1509
BspCNI CTCAG 4 cut(s) 102, 583, 924, 1210
BspFNI CGCG 5 cut(s) 407, 409, 502, 751, 1512
BspHI TCATGA 1 cut(s) 1041
BspLI GGNNCC 4 cut(s) 155, 482, 633, 1161
BspMI ACCTGC 2 cut(s) 377, 1421
BspQI GCTCTTC 1 cut(s) 915
BspT104I TTCGAA 1 cut(s) 913
BsrGI TGTACA 1 cut(s) 765
BsrI ACTGG 3 cut(s) 366, 716, 792
BssECI CCNNGG 5 cut(s) 31, 191, 976, 1067, 1189
BssHII GCGCGC 2 cut(s) 405, 407
BssMI GATC 3 cut(s) 1096, 1461, 1482
BssNAI GTATAC 1 cut(s) 252
BssSI CACGAG 1 cut(s) 856
BssT1I CCWWGG 3 cut(s) 31, 976, 1067
Bst1107I GTATAC 1 cut(s) 252
Bst2BI CACGAG 1 cut(s) 856
Bst2UI CCWGG 2 cut(s) 641, 1383
Bst4CI ACNGT 3 cut(s) 148, 853, 1252
Bst6I CTCTTC 3 cut(s) 125, 717, 915
BstAPI GCANNNNNTGC 1 cut(s) 1232
BstAUI TGTACA 1 cut(s) 765
BstBAI YACGTR 2 cut(s) 255, 1363
BstBI TTCGAA 1 cut(s) 913
BstC8I GCNNGC 8 cut(s) 178, 222, 277, 407, 409, 1202, 1224, 1494
BstDEI CTNAG 6 cut(s) 63, 110, 570, 744, 932, 1218
BstDSI CCRYGG 1 cut(s) 31
BstF5I GGATG 4 cut(s) 250, 297, 618, 631
BstFNI CGCG 5 cut(s) 407, 409, 502, 751, 1512
BstHHI GCGC 7 cut(s) 109, 281, 407, 409, 411, 502, 649
BstKTI GATC 3 cut(s) 1099, 1464, 1485
BstMAI GTCTC 3 cut(s) 859, 1139, 1339
BstMBI GATC 3 cut(s) 1096, 1461, 1482
BstMWI GCNNNNNNNGC 5 cut(s) 566, 653, 1159, 1232, 1498
BstNI CCWGG 2 cut(s) 641, 1383
BstNSI RCATGY 1 cut(s) 1204
BstSCI CCNGG 4 cut(s) 190, 483, 639, 1381
BstSFI CTRYAG 1 cut(s) 994
BstSNI TACGTA 1 cut(s) 255
BstUI CGCG 5 cut(s) 407, 409, 502, 751, 1512
BstV1I GCAGC 4 cut(s) 192, 447, 1073, 1247
BstZ17I GTATAC 1 cut(s) 252
BsuRI GGCC 2 cut(s) 668, 1509
BtgI CCRYGG 1 cut(s) 31
BtsCI GGATG 4 cut(s) 250, 297, 618, 631
BtsIMutI CAGTG 2 cut(s) 709, 849
BveI ACCTGC 2 cut(s) 377, 1421
Cac8I GCNNGC 8 cut(s) 178, 222, 277, 407, 409, 1202, 1224, 1494
CciI TCATGA 1 cut(s) 1041
CfoI GCGC 7 cut(s) 109, 281, 407, 409, 411, 502, 649
Cfr13I GGNCC 1 cut(s) 637
CseI GACGC 2 cut(s) 1054, 1178
CsiI ACCWGGT 1 cut(s) 639
Csp6I GTAC 5 cut(s) 68, 141, 715, 766, 787
CviAII CATG 9 cut(s) 32, 317, 1001, 1042, 1090, 1133, 1201, 1418, 1451
CviQI GTAC 5 cut(s) 68, 141, 715, 766, 787
DdeI CTNAG 6 cut(s) 63, 110, 570, 744, 932, 1218
DpnI GATC 3 cut(s) 1098, 1463, 1484
DpnII GATC 3 cut(s) 1096, 1461, 1482
DraI TTTAAA 1 cut(s) 1122
Eam1104I CTCTTC 3 cut(s) 125, 717, 915
EarI CTCTTC 3 cut(s) 125, 717, 915
EciI GGCGGA 2 cut(s) 625, 638
Ecl136II GAGCTC 1 cut(s) 936
Eco105I TACGTA 1 cut(s) 255
Eco130I CCWWGG 3 cut(s) 31, 976, 1067
Eco24I GRGCYC 1 cut(s) 938
Eco47I GGWCC 1 cut(s) 637
Eco53kI GAGCTC 1 cut(s) 936
Eco72I CACGTG 1 cut(s) 1363
EcoICRI GAGCTC 1 cut(s) 936
EcoRI GAATTC 1 cut(s) 1213
EcoRII CCWGG 2 cut(s) 639, 1381
EcoT14I CCWWGG 3 cut(s) 31, 976, 1067
EcoT38I GRGCYC 1 cut(s) 938
ErhI CCWWGG 3 cut(s) 31, 976, 1067
FaeI CATG 9 cut(s) 35, 320, 1004, 1045, 1093, 1136, 1204, 1421, 1454
FaqI GGGAC 2 cut(s) 698, 1071
FatI CATG 9 cut(s) 31, 316, 1000, 1041, 1089, 1132, 1200, 1417, 1450
FauI CCCGC 2 cut(s) 553, 890
FauNDI CATATG 1 cut(s) 783
FblI GTMKAC 1 cut(s) 251
Fnu4HI GCNGC 7 cut(s) 181, 461, 503, 1087, 1236, 1275, 1510
FokI GGATG 4 cut(s) 257, 304, 625, 638
FriOI GRGCYC 1 cut(s) 938
Fsp4HI GCNGC 7 cut(s) 181, 461, 503, 1087, 1236, 1275, 1510
FspBI CTAG 6 cut(s) 71, 282, 650, 840, 1328, 1549
GlaI GCGC 7 cut(s) 108, 280, 406, 408, 410, 501, 648
GluI GCNGC 7 cut(s) 181, 461, 503, 1087, 1236, 1275, 1510
GsaI CCCAGC 1 cut(s) 1501
GsuI CTGGAG 1 cut(s) 1365
HaeIII GGCC 2 cut(s) 668, 1509
HapII CCGG 5 cut(s) 191, 242, 484, 524, 635
HgaI GACGC 2 cut(s) 1054, 1178
HhaI GCGC 7 cut(s) 109, 281, 407, 409, 411, 502, 649
Hin1II CATG 9 cut(s) 35, 320, 1004, 1045, 1093, 1136, 1204, 1421, 1454
Hin6I GCGC 7 cut(s) 107, 279, 405, 407, 409, 500, 647
HinP1I GCGC 7 cut(s) 107, 279, 405, 407, 409, 500, 647
HindIII AAGCTT 1 cut(s) 591
HinfI GANTC 4 cut(s) 36, 133, 1059, 1454
HpaII CCGG 5 cut(s) 191, 242, 484, 524, 635
HphI GGTGA 4 cut(s) 31, 238, 769, 1445
Hpy166II GTNNAC 5 cut(s) 252, 546, 709, 861, 1360
Hpy188I TCNGA 6 cut(s) 138, 167, 363, 573, 940, 1219
Hpy188III TCNNGA 3 cut(s) 98, 121, 1042
Hpy8I GTNNAC 5 cut(s) 252, 546, 709, 861, 1360
HpyAV CCTTC 6 cut(s) 190, 613, 693, 837, 1020, 1499
HpyCH4III ACNGT 3 cut(s) 148, 853, 1252
HpyCH4IV ACGT 3 cut(s) 254, 717, 1362
HpyCH4V TGCA 8 cut(s) 316, 386, 773, 1089, 1200, 1226, 1235, 1256
HpyF10VI GCNNNNNNNGC 5 cut(s) 566, 653, 1159, 1232, 1498
HpyF3I CTNAG 6 cut(s) 63, 110, 570, 744, 932, 1218
HpySE526I ACGT 3 cut(s) 254, 717, 1362
Hsp92II CATG 9 cut(s) 35, 320, 1004, 1045, 1093, 1136, 1204, 1421, 1454
HspAI GCGC 7 cut(s) 107, 279, 405, 407, 409, 500, 647
Kzo9I GATC 3 cut(s) 1096, 1461, 1482
LguI GCTCTTC 1 cut(s) 915
LmnI GCTCC 5 cut(s) 307, 480, 846, 1159, 1409
Lsp1109I GCAGC 4 cut(s) 192, 447, 1073, 1247
LweI GCATC 5 cut(s) 510, 825, 876, 918, 1235
MabI ACCWGGT 1 cut(s) 639
MaeI CTAG 6 cut(s) 71, 282, 650, 840, 1328, 1549
MaeII ACGT 3 cut(s) 254, 717, 1362
MaeIII GTNAC 2 cut(s) 226, 757
MalI GATC 3 cut(s) 1098, 1463, 1484
MboI GATC 3 cut(s) 1096, 1461, 1482
MboII GAAGA 4 cut(s) 142, 734, 902, 1384
MfeI CAATTG 1 cut(s) 923
MhlI GDGCHC 6 cut(s) 571, 851, 938, 1080, 1186, 1293
MluCI AATT 6 cut(s) 9, 678, 923, 966, 1213, 1243
MlyI GAGTC 2 cut(s) 142, 1053
MmeI TCCRAC 1 cut(s) 431
MroXI GAANNNNTTC 1 cut(s) 909
MseI TTAA 4 cut(s) 1103, 1121, 1247, 1526
MslI CAYNNNNRTG 2 cut(s) 782, 1449
MspI CCGG 5 cut(s) 191, 242, 484, 524, 635
MspR9I CCNGG 4 cut(s) 192, 485, 641, 1383
MunI CAATTG 1 cut(s) 923
MvaI CCWGG 2 cut(s) 641, 1383
MvnI CGCG 5 cut(s) 407, 409, 502, 751, 1512
MwoI GCNNNNNNNGC 5 cut(s) 566, 653, 1159, 1232, 1498
NciI CCSGG 2 cut(s) 192, 485
NcoI CCATGG 1 cut(s) 31
NdeI CATATG 1 cut(s) 783
NdeII GATC 3 cut(s) 1096, 1461, 1482
NlaIII CATG 9 cut(s) 35, 320, 1004, 1045, 1093, 1136, 1204, 1421, 1454
NlaIV GGNNCC 4 cut(s) 155, 482, 633, 1161
NmeAIII GCCGAG 2 cut(s) 262, 1170
NmuCI GTSAC 2 cut(s) 226, 757
NspI RCATGY 1 cut(s) 1204
NspV TTCGAA 1 cut(s) 913
PaeI GCATGC 1 cut(s) 1204
PagI TCATGA 1 cut(s) 1041
PaqCI CACCTGC 2 cut(s) 377, 1421
PauI GCGCGC 2 cut(s) 405, 407
PciSI GCTCTTC 1 cut(s) 915
PdmI GAANNNNTTC 1 cut(s) 909
PfeI GAWTC 2 cut(s) 36, 1454
PkrI GCNGC 7 cut(s) 182, 462, 504, 1088, 1237, 1276, 1511
PleI GAGTC 2 cut(s) 141, 1053
PmaCI CACGTG 1 cut(s) 1363
PmlI CACGTG 1 cut(s) 1363
PpsI GAGTC 2 cut(s) 141, 1053
Ppu21I YACGTR 2 cut(s) 255, 1363
Psp124BI GAGCTC 1 cut(s) 938
Psp6I CCWGG 2 cut(s) 639, 1381
PspCI CACGTG 1 cut(s) 1363
PspFI CCCAGC 1 cut(s) 1497
PspGI CCWGG 2 cut(s) 639, 1381
PspN4I GGNNCC 4 cut(s) 155, 482, 633, 1161
PspPI GGNCC 1 cut(s) 637
PsrI GAACNNNNNNTAC 2 cut(s) 809, 841
PteI GCGCGC 2 cut(s) 405, 407
RsaI GTAC 5 cut(s) 69, 142, 716, 767, 788
RsaNI GTAC 5 cut(s) 68, 141, 715, 766, 787
RseI CAYNNNNRTG 2 cut(s) 782, 1449
SacI GAGCTC 1 cut(s) 938
SapI GCTCTTC 1 cut(s) 915
SaqAI TTAA 4 cut(s) 1103, 1121, 1247, 1526
SatI GCNGC 7 cut(s) 181, 461, 503, 1087, 1236, 1275, 1510
Sau3AI GATC 3 cut(s) 1096, 1461, 1482
Sau96I GGNCC 1 cut(s) 637
ScaI AGTACT 1 cut(s) 142
SchI GAGTC 2 cut(s) 142, 1053
ScrFI CCNGG 4 cut(s) 192, 485, 641, 1383
SduI GDGCHC 6 cut(s) 571, 851, 938, 1080, 1186, 1293
SexAI ACCWGGT 1 cut(s) 639
SfaNI GCATC 5 cut(s) 510, 825, 876, 918, 1235
SfcI CTRYAG 1 cut(s) 994
SfuI TTCGAA 1 cut(s) 913
SinI GGWCC 1 cut(s) 637
SmiMI CAYNNNNRTG 2 cut(s) 782, 1449
SmlI CTYRAG 1 cut(s) 688
SmoI CTYRAG 1 cut(s) 688
SnaBI TACGTA 1 cut(s) 255
SphI GCATGC 1 cut(s) 1204
Sse9I AATT 6 cut(s) 9, 678, 923, 966, 1213, 1243
SspI AATATT 1 cut(s) 918
SspMI CTAG 6 cut(s) 71, 282, 650, 840, 1328, 1549
SstI GAGCTC 1 cut(s) 938
StyD4I CCNGG 4 cut(s) 190, 483, 639, 1381
StyI CCWWGG 3 cut(s) 31, 976, 1067
TaaI ACNGT 3 cut(s) 148, 853, 1252
TaiI ACGT 3 cut(s) 257, 720, 1365
TaqI TCGA 5 cut(s) 913, 1062, 1095, 1298, 1387
TasI AATT 6 cut(s) 9, 678, 923, 966, 1213, 1243
TatI WGTACW 2 cut(s) 140, 765
TauI GCSGC 3 cut(s) 505, 1277, 1512
TfiI GAWTC 2 cut(s) 36, 1454
Tru1I TTAA 4 cut(s) 1103, 1121, 1247, 1526
Tru9I TTAA 4 cut(s) 1103, 1121, 1247, 1526
TscAI CASTG 2 cut(s) 716, 856
TseFI GTSAC 2 cut(s) 226, 757
TseI GCWGC 4 cut(s) 180, 460, 1086, 1235
Tsp45I GTSAC 2 cut(s) 226, 757
TspDTI ATGAA 8 cut(s) 438, 825, 978, 1030, 1140, 1406, 1467, 1482
TspGWI ACGGA 1 cut(s) 245
TspRI CASTG 2 cut(s) 716, 856
VpaK11BI GGWCC 1 cut(s) 637
XapI RAATTY 3 cut(s) 9, 1213, 1243
XceI RCATGY 1 cut(s) 1204
XcmI CCANNNNNNNNNTGG 2 cut(s) 799, 1389
XmiI GTMKAC 1 cut(s) 251
XmnI GAANNNNTTC 1 cut(s) 909
XspI CTAG 6 cut(s) 71, 282, 650, 840, 1328, 1549
ZrmI AGTACT 1 cut(s) 142
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.