pycom01g05700

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0010203
Physical Location & Seq
Reverse (-)
5564488 .. 5566388
1901 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g05700.1

Sequence Viewer

Length: 684 bp
ATGACTCAAGGTGCGATTCAGGTTTTGGGGCACATGAGGGTTCATGGGTTTAAGCCCCACTTGCACGCTTGTACTGTCTTATTGAGTTCTTTGGTTAAACATAGGTTGGACCAACATGGTGTGGAAAGTTTACAAGAATATGATCAACGCCGGGATGTTCATTTGTACACTGTGTTGATTCATGCTTGTTGCAAGTCTGGGGGTGTAGAGAAGGCAGAGAGTTTGCTGAGTGAGATGGAATTCAAGTGTGCCTTTCCTGATCTTTTCACATACAATACGTTGATATCGCTGTATTCTAAAAGGGGTATGCACTACGAAGCTTTGTCTGTTCGAGATAGAATGGAAAGAGCAGGAGTTACCCCTGATATGGTGACGTACAATTCTCTTATGTATGCGTTTTGTAGAGAAGATATACTGATTTTGATCGCGGTTTTGGTGGCGGCAACACAATTAATCAGAATGTGTTGTTTGGGGGGGGGGGGGGGGGGGGGGGTGGTGCTGGGGTTGGTTTTAAATTATACTTGGAAAGCATCTGTTTGGGCTGTTCTGTGGAGATGTGCATCCTTCCATGAGTGGGACAGTTGGCTTGCATTTTCCAAAGCATCAAGTCGAGGTGTAGGCCGAACAGAAAAATATGTGGAATGGGGGTCTTCAAAATCATATGTGACAGATGATTTGTATTAA

Protein Analysis

228

Amino Acids

25.54

Weight (kDa)

7.63

Isoelectric Point (pI)

34.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_3 PF13812 46 - 101 1.5e-06 Pentatricopeptide repeat domain
PPR_2 PF13041 52 - 92 2.3e-09 PPR repeat family
PPR_1 PF12854 52 - 80 9.5e-07 PPR repeat
PPR_long PF17177 55 - 130 2.8e-06 Pentacotripeptide-repeat region of PRORP
PPR PF01535 56 - 80 1.2e-06 PPR repeat
PPR_3 PF13812 74 - 133 9e-10 Pentatricopeptide repeat domain
PPR_2 PF13041 86 - 135 4.2e-15 PPR repeat family
PPR PF01535 89 - 119 5e-08 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 428
AciI CCGC 2 cut(s) 428, 440
AcsI RAATTY 1 cut(s) 239
AfaI GTAC 3 cut(s) 73, 167, 377
AfiI CCNNNNNNNGG 2 cut(s) 367, 574
AgsI TTSAA 2 cut(s) 244, 654
AluBI AGCT 1 cut(s) 320
AluI AGCT 1 cut(s) 320
AoxI GGCC 1 cut(s) 619
ApoI RAATTY 1 cut(s) 239
ArsI GACNNNNNNTTYG 1 cut(s) 658
AseI ATTAAT 1 cut(s) 452
AspS9I GGNCC 1 cut(s) 109
AsuC2I CCSGG 1 cut(s) 152
AsuHPI GGTGA 1 cut(s) 382
AvaII GGWCC 1 cut(s) 109
BaeGI GKGCMC 1 cut(s) 33
BbsI GAAGAC 1 cut(s) 642
BccI CCATC 1 cut(s) 229
BclI TGATCA 1 cut(s) 142
BcnI CCSGG 1 cut(s) 152
BisI GCNGC 1 cut(s) 441
BlsI GCNGC 1 cut(s) 442
Bme1390I CCNGG 1 cut(s) 152
Bme18I GGWCC 1 cut(s) 109
BmgT120I GGNCC 1 cut(s) 109
BmrFI CCNGG 1 cut(s) 152
BmsI GCATC 3 cut(s) 539, 569, 611
BpiI GAAGAC 1 cut(s) 642
BpuMI CCSGG 1 cut(s) 152
BsaBI GATNNNNATC 2 cut(s) 422, 559
Bsc4I CCNNNNNNNGG 2 cut(s) 367, 574
Bse8I GATNNNNATC 2 cut(s) 422, 559
BseGI GGATG 2 cut(s) 160, 560
BseJI GATNNNNATC 2 cut(s) 422, 559
BseLI CCNNNNNNNGG 2 cut(s) 367, 574
BseMII CTCAG 1 cut(s) 218
BseSI GKGCMC 1 cut(s) 33
BseYI CCCAGC 1 cut(s) 499
Bsh1236I CGCG 1 cut(s) 428
BshFI GGCC 1 cut(s) 621
BsiSI CCGG 1 cut(s) 151
BslFI GGGAC 1 cut(s) 590
BslI CCNNNNNNNGG 2 cut(s) 367, 574
BsmFI GGGAC 1 cut(s) 590
BsnI GGCC 1 cut(s) 621
Bsp1286I GDGCHC 1 cut(s) 33
Bsp1407I TGTACA 1 cut(s) 165
Bsp143I GATC 3 cut(s) 142, 259, 423
BspACI CCGC 2 cut(s) 428, 440
BspANI GGCC 1 cut(s) 621
BspCNI CTCAG 1 cut(s) 219
BspFNI CGCG 1 cut(s) 428
BsrGI TGTACA 1 cut(s) 165
BssMI GATC 3 cut(s) 142, 259, 423
Bst4CI ACNGT 3 cut(s) 76, 172, 581
BstAUI TGTACA 1 cut(s) 165
BstC8I GCNNGC 2 cut(s) 66, 588
BstDEI CTNAG 1 cut(s) 227
BstF5I GGATG 2 cut(s) 160, 560
BstFNI CGCG 1 cut(s) 428
BstKTI GATC 3 cut(s) 145, 262, 426
BstMBI GATC 3 cut(s) 142, 259, 423
BstMWI GCNNNNNNNGC 1 cut(s) 61
BstSCI CCNGG 1 cut(s) 150
BstSLI GKGCMC 1 cut(s) 33
BstUI CGCG 1 cut(s) 428
BstV2I GAAGAC 1 cut(s) 642
BsuRI GGCC 1 cut(s) 621
BtsCI GGATG 2 cut(s) 160, 560
BtsIMutI CAGTG 1 cut(s) 168
Cac8I GCNNGC 2 cut(s) 66, 588
Cfr13I GGNCC 1 cut(s) 109
Csp6I GTAC 3 cut(s) 72, 166, 376
CviAII CATG 5 cut(s) 34, 44, 116, 182, 569
CviJI RGCY 5 cut(s) 55, 320, 542, 586, 621
CviKI_1 RGCY 5 cut(s) 55, 320, 542, 586, 621
CviQI GTAC 3 cut(s) 72, 166, 376
DdeI CTNAG 1 cut(s) 227
DpnI GATC 3 cut(s) 144, 261, 425
DpnII GATC 3 cut(s) 142, 259, 423
DraI TTTAAA 1 cut(s) 513
Eco32I GATATC 1 cut(s) 285
Eco47I GGWCC 1 cut(s) 109
EcoRI GAATTC 1 cut(s) 239
EcoRV GATATC 1 cut(s) 285
FaeI CATG 5 cut(s) 37, 47, 119, 185, 572
FalI AAGNNNNNCTT 4 cut(s) 44, 76, 236, 268
FaqI GGGAC 1 cut(s) 590
FatI CATG 5 cut(s) 33, 43, 115, 181, 568
FauNDI CATATG 1 cut(s) 661
FbaI TGATCA 1 cut(s) 142
Fnu4HI GCNGC 1 cut(s) 441
FokI GGATG 2 cut(s) 167, 547
Fsp4HI GCNGC 1 cut(s) 441
GluI GCNGC 1 cut(s) 441
GsaI CCCAGC 1 cut(s) 503
HaeIII GGCC 1 cut(s) 621
HapII CCGG 1 cut(s) 151
Hin1II CATG 5 cut(s) 37, 47, 119, 185, 572
HindIII AAGCTT 1 cut(s) 318
HinfI GANTC 3 cut(s) 4, 16, 178
HpaII CCGG 1 cut(s) 151
HphI GGTGA 1 cut(s) 382
Hpy166II GTNNAC 2 cut(s) 131, 168
Hpy188I TCNGA 1 cut(s) 458
Hpy188III TCNNGA 2 cut(s) 257, 332
Hpy8I GTNNAC 2 cut(s) 131, 168
HpyAV CCTTC 2 cut(s) 205, 574
HpyCH4III ACNGT 3 cut(s) 76, 172, 581
HpyCH4IV ACGT 2 cut(s) 278, 374
HpyCH4V TGCA 5 cut(s) 64, 192, 310, 560, 590
HpyF10VI GCNNNNNNNGC 1 cut(s) 61
HpyF3I CTNAG 1 cut(s) 227
HpySE526I ACGT 2 cut(s) 278, 374
Hsp92II CATG 5 cut(s) 37, 47, 119, 185, 572
Ksp22I TGATCA 1 cut(s) 142
Kzo9I GATC 3 cut(s) 142, 259, 423
LpnPI CCDG 7 cut(s) 5, 164, 183, 270, 336, 375, 485
LweI GCATC 3 cut(s) 539, 569, 611
MaeII ACGT 2 cut(s) 278, 374
MaeIII GTNAC 3 cut(s) 355, 370, 664
MalI GATC 3 cut(s) 144, 261, 425
MboI GATC 3 cut(s) 142, 259, 423
MboII GAAGA 2 cut(s) 419, 642
MhlI GDGCHC 1 cut(s) 33
MluCI AATT 4 cut(s) 239, 379, 449, 514
MmeI TCCRAC 1 cut(s) 87
MnlI CCTC 2 cut(s) 30, 605
MseI TTAA 5 cut(s) 51, 96, 452, 512, 682
MspI CCGG 1 cut(s) 151
MspR9I CCNGG 1 cut(s) 152
MvnI CGCG 1 cut(s) 428
MwoI GCNNNNNNNGC 1 cut(s) 61
NciI CCSGG 1 cut(s) 152
NdeI CATATG 1 cut(s) 661
NdeII GATC 3 cut(s) 142, 259, 423
NlaIII CATG 5 cut(s) 37, 47, 119, 185, 572
NmuCI GTSAC 2 cut(s) 370, 664
PfeI GAWTC 2 cut(s) 16, 178
PkrI GCNGC 1 cut(s) 442
PshBI ATTAAT 1 cut(s) 452
PspFI CCCAGC 1 cut(s) 499
PspPI GGNCC 1 cut(s) 109
RsaI GTAC 3 cut(s) 73, 167, 377
RsaNI GTAC 3 cut(s) 72, 166, 376
SaqAI TTAA 5 cut(s) 51, 96, 452, 512, 682
SatI GCNGC 1 cut(s) 441
Sau3AI GATC 3 cut(s) 142, 259, 423
Sau96I GGNCC 1 cut(s) 109
ScrFI CCNGG 1 cut(s) 152
SduI GDGCHC 1 cut(s) 33
SetI ASST 7 cut(s) 13, 24, 107, 281, 322, 377, 616
SfaNI GCATC 3 cut(s) 539, 569, 611
SinI GGWCC 1 cut(s) 109
SmlI CTYRAG 1 cut(s) 6
SmoI CTYRAG 1 cut(s) 6
Sse9I AATT 4 cut(s) 239, 379, 449, 514
SsiI CCGC 2 cut(s) 428, 440
StyD4I CCNGG 1 cut(s) 150
TaaI ACNGT 3 cut(s) 76, 172, 581
TaiI ACGT 2 cut(s) 281, 377
TaqI TCGA 2 cut(s) 331, 610
TasI AATT 4 cut(s) 239, 379, 449, 514
TatI WGTACW 2 cut(s) 71, 165
TauI GCSGC 1 cut(s) 443
TfiI GAWTC 2 cut(s) 16, 178
Tru1I TTAA 5 cut(s) 51, 96, 452, 512, 682
Tru9I TTAA 5 cut(s) 51, 96, 452, 512, 682
TscAI CASTG 1 cut(s) 175
TseFI GTSAC 2 cut(s) 370, 664
Tsp45I GTSAC 2 cut(s) 370, 664
TspDTI ATGAA 3 cut(s) 32, 149, 170
TspRI CASTG 1 cut(s) 175
VpaK11BI GGWCC 1 cut(s) 109
VspI ATTAAT 1 cut(s) 452
XapI RAATTY 1 cut(s) 239
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.