pycom01g12910

Belongs to the peptidase S10 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0007698
Physical Location & Seq
Forward (+)
13522898 .. 13525724
2827 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1209 bp
ATGCCGCCAAGTGAGATGTTCTATCTCTTCTTCGAGTCTCGCACCAACAAGAAGGACCCTGTTGTTATTTGGTTGACTGGCGGTCCGGGGTGCAGCAGTGAATTGGCTGTATTTTACGAAAATGGTCCTTTCACTATCGCAAATAACTTGTCACTTGTGTGGAACGAGTATGGTTGGGATAAGGCATCTAATCTTCTGTATGTTGACCAGCCTATTGGGACTGGCTTCAGTTATAGTAGTGACAAACGTGACATTCGTCACTCTGAAGATGGTGTTAGCAATGACCTTTATGACTTCTTACAGGCCTTCTTTGCCGAGCACCCTGAGTTGGCAAAGAATGACTTTTTCATAACTGGAGAGTCATATGCTGGACACTATATTCCAGCTTTTGCTGCTCGAGTCCACAAAGGAAACAAAGCCAAAGAAGGAATCCATATAAATTTGAAGGGATTTGCCATTGGCAATGGGCTTACTGATCCTGCAGTCCAGTATAAGGCTTACACAGATTTTGCGTTGGACAATGGCTTAATCACAGCAACTGAATATAATCGTATTAACAAAGTGCTTCCAGTTTGTGAAATGGCTATAAAGCTCTGTGGCACTGATGGTACAGTCTCTTGCATGGCTTCATATTTTGTTTGCAATACCATATTCAGTAGCATCATGGCGAAGGCCGGAAACATAAATTACTATGACATCAGACAGAAGTGTGAGGGGAGCCTTTGCTATGACTTCTCAAACATGGAGAAATTCCTGAACCTGAAGCCTGTTAGAGAGTCACTTGGAGTTGGGGACATCGAATTTGTTTCATGCAGCTCTACAGTGTATGAAGCCATGCTGGTGGACTGGATGAGGAACCTCGAAGTGGGCATCCCCACTCTTCTCGAGGACGGAATCCAATTGCTTGTGTATGCAGGAGAATATGATCTAATCTGCAACTGGCTTGGTAACTCAAGATGGGTCCATGCCATGGAATGGTCTGGTCAGAAACAGTTTGTAACTTCTCCGGAAGTTCCTTTTGTTGTCGATAAGTCAGAAGCAGGAGTTCTCAAGAACCATGGGCCTCTTAGCTTCCTTAAGGTCCATGATGCCGGCCACATGGTTCCGATGGACCAGCCCAAGGCTGCACTGGAGATGTTGAGGAGGTGGACTCGGGGATCAATTGCTGAATCCGCAGGTGAACCAGAAAAACAGGTTGCTCAGATGTGA

Protein Analysis

403

Amino Acids

44.9

Weight (kDa)

5.08

Isoelectric Point (pI)

40.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S10 PF00450 4 - 385 2.7e-122 Serine carboxypeptidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1166
Acc36I ACCTGC 1 cut(s) 1166
AccB7I CCANNNNNTGG 2 cut(s) 970, 975
AccIII TCCGGA 1 cut(s) 1006
AciI CCGC 3 cut(s) 5, 81, 1173
AclWI GGATC 2 cut(s) 470, 1165
AcoI YGGCCR 1 cut(s) 1093
AcsI RAATTY 3 cut(s) 439, 749, 800
AcuI CTGAAG 3 cut(s) 211, 285, 782
AfaI GTAC 1 cut(s) 610
AfiI CCNNNNNNNGG 6 cut(s) 328, 493, 865, 970, 975, 1120
AflII CTTAAG 1 cut(s) 1076
AgsI TTSAA 1 cut(s) 445
AhdI GACNNNNNGTC 1 cut(s) 81
AleI CACNNNNGTG 1 cut(s) 157
AluBI AGCT 4 cut(s) 386, 592, 816, 1071
AluI AGCT 4 cut(s) 386, 592, 816, 1071
Alw21I GWGCWC 1 cut(s) 321
Alw26I GTCTC 2 cut(s) 42, 619
AlwI GGATC 2 cut(s) 470, 1165
AlwNI CAGNNNCTG 1 cut(s) 539
Ama87I CYCGRG 3 cut(s) 396, 884, 1152
Aor13HI TCCGGA 1 cut(s) 1006
AoxI GGCC 4 cut(s) 303, 672, 1061, 1093
ApeKI GCWGC 4 cut(s) 93, 392, 813, 1124
ApoI RAATTY 3 cut(s) 439, 749, 800
ArsI GACNNNNNNTTYG 4 cut(s) 134, 166, 785, 817
AspS9I GGNCC 7 cut(s) 55, 83, 125, 961, 1061, 1081, 1111
AsuC2I CCSGG 1 cut(s) 87
AsuHPI GGTGA 1 cut(s) 1190
AvaI CYCGRG 3 cut(s) 396, 884, 1152
AvaII GGWCC 6 cut(s) 55, 83, 125, 961, 1081, 1111
Bbv12I GWGCWC 1 cut(s) 321
BbvI GCAGC 4 cut(s) 105, 379, 825, 1111
BccI CCATC 4 cut(s) 263, 599, 951, 1102
BcnI CCSGG 1 cut(s) 87
BcoDI GTCTC 2 cut(s) 42, 619
BfmI CTRYAG 2 cut(s) 480, 819
BfrI CTTAAG 1 cut(s) 1076
BfuAI ACCTGC 1 cut(s) 1166
BisI GCNGC 5 cut(s) 5, 94, 393, 814, 1125
BlsI GCNGC 5 cut(s) 6, 95, 394, 815, 1126
Bme1390I CCNGG 1 cut(s) 87
Bme18I GGWCC 6 cut(s) 55, 83, 125, 961, 1081, 1111
BmeRI GACNNNNNGTC 1 cut(s) 81
BmeT110I CYCGRG 3 cut(s) 396, 884, 1152
BmgT120I GGNCC 7 cut(s) 55, 83, 125, 961, 1061, 1081, 1111
BmiI GGNNCC 5 cut(s) 57, 719, 857, 962, 1104
BmrFI CCNGG 1 cut(s) 87
BmsI GCATC 4 cut(s) 194, 669, 879, 1078
BoxI GACNNNNGTC 1 cut(s) 255
BplI GAGNNNNNCTC 2 cut(s) 1135, 1167
BpmI CTGGAG 2 cut(s) 375, 1151
BpuEI CTTGAG 2 cut(s) 937, 1034
BpuMI CCSGG 1 cut(s) 87
BsaJI CCNNGG 4 cut(s) 86, 969, 1057, 1119
BsaWI WCCGGW 1 cut(s) 1006
Bsc4I CCNNNNNNNGG 6 cut(s) 328, 493, 865, 970, 975, 1120
Bse118I RCCGGY 1 cut(s) 1091
Bse1I ACTGG 8 cut(s) 82, 226, 358, 487, 569, 851, 944, 1134
Bse3DI GCAATG 2 cut(s) 286, 469
BseAI TCCGGA 1 cut(s) 1006
BseDI CCNNGG 4 cut(s) 86, 969, 1057, 1119
BseGI GGATG 2 cut(s) 855, 870
BseLI CCNNNNNNNGG 6 cut(s) 328, 493, 865, 970, 975, 1120
BseMI GCAATG 2 cut(s) 286, 469
BseMII CTCAG 1 cut(s) 315
BseNI ACTGG 8 cut(s) 82, 226, 358, 487, 569, 851, 944, 1134
BseRI GAGGAG 1 cut(s) 1156
BseXI GCAGC 4 cut(s) 105, 379, 825, 1111
BsgI GTGCAG 2 cut(s) 112, 1110
BshFI GGCC 4 cut(s) 305, 674, 1063, 1095
BsiHKAI GWGCWC 1 cut(s) 321
BsiHKCI CYCGRG 3 cut(s) 396, 884, 1152
BsiSI CCGG 4 cut(s) 86, 675, 1007, 1092
BslFI GGGAC 2 cut(s) 232, 806
BslI CCNNNNNNNGG 6 cut(s) 328, 493, 865, 970, 975, 1120
BsmAI GTCTC 2 cut(s) 42, 619
BsmFI GGGAC 2 cut(s) 232, 806
BsnI GGCC 4 cut(s) 305, 674, 1063, 1095
BsoBI CYCGRG 3 cut(s) 396, 884, 1152
Bsp1286I GDGCHC 1 cut(s) 321
Bsp13I TCCGGA 1 cut(s) 1006
Bsp143I GATC 3 cut(s) 475, 925, 1157
Bsp19I CCATGG 2 cut(s) 969, 1057
BspACI CCGC 3 cut(s) 5, 81, 1173
BspANI GGCC 4 cut(s) 305, 674, 1063, 1095
BspCNI CTCAG 1 cut(s) 316
BspEI TCCGGA 1 cut(s) 1006
BspLI GGNNCC 5 cut(s) 57, 719, 857, 962, 1104
BspMAI CTGCAG 1 cut(s) 484
BspMI ACCTGC 1 cut(s) 1166
BspPI GGATC 2 cut(s) 470, 1165
BspTI CTTAAG 1 cut(s) 1076
BsrDI GCAATG 2 cut(s) 286, 469
BsrFI RCCGGY 1 cut(s) 1091
BsrI ACTGG 8 cut(s) 82, 226, 358, 487, 569, 851, 944, 1134
BssAI RCCGGY 1 cut(s) 1091
BssECI CCNNGG 4 cut(s) 86, 969, 1057, 1119
BssMI GATC 3 cut(s) 475, 925, 1157
BssT1I CCWWGG 3 cut(s) 969, 1057, 1119
Bst4CI ACNGT 3 cut(s) 613, 823, 993
Bst6I CTCTTC 2 cut(s) 32, 885
BstAFI CTTAAG 1 cut(s) 1076
BstC8I GCNNGC 1 cut(s) 1093
BstDEI CTNAG 3 cut(s) 324, 1067, 1200
BstDSI CCRYGG 2 cut(s) 969, 1057
BstF5I GGATG 2 cut(s) 855, 870
BstKTI GATC 3 cut(s) 478, 928, 1160
BstMAI GTCTC 2 cut(s) 42, 619
BstMBI GATC 3 cut(s) 475, 925, 1157
BstMWI GCNNNNNNNGC 3 cut(s) 311, 392, 1172
BstPAI GACNNNNGTC 1 cut(s) 255
BstSCI CCNGG 1 cut(s) 85
BstSFI CTRYAG 2 cut(s) 480, 819
BstV1I GCAGC 4 cut(s) 105, 379, 825, 1111
BstXI CCANNNNNNTGG 2 cut(s) 215, 841
BsuRI GGCC 4 cut(s) 305, 674, 1063, 1095
BtgI CCRYGG 2 cut(s) 969, 1057
BtsCI GGATG 2 cut(s) 855, 870
BtsI GCAGTG 1 cut(s) 103
BtsIMutI CAGTG 4 cut(s) 103, 600, 828, 1127
BveI ACCTGC 1 cut(s) 1166
Cac8I GCNNGC 1 cut(s) 1093
CaiI CAGNNNCTG 1 cut(s) 539
Cfr10I RCCGGY 1 cut(s) 1091
Cfr13I GGNCC 7 cut(s) 55, 83, 125, 961, 1061, 1081, 1111
CpoI CGGWCCG 1 cut(s) 83
Csp6I GTAC 1 cut(s) 609
CspI CGGWCCG 1 cut(s) 83
CviQI GTAC 1 cut(s) 609
DdeI CTNAG 3 cut(s) 324, 1067, 1200
DpnI GATC 3 cut(s) 477, 927, 1159
DpnII GATC 3 cut(s) 475, 925, 1157
DriI GACNNNNNGTC 1 cut(s) 81
EaeI YGGCCR 1 cut(s) 1093
Eam1104I CTCTTC 2 cut(s) 32, 885
Eam1105I GACNNNNNGTC 1 cut(s) 81
EarI CTCTTC 2 cut(s) 32, 885
Eco130I CCWWGG 3 cut(s) 969, 1057, 1119
Eco147I AGGCCT 1 cut(s) 305
Eco47I GGWCC 6 cut(s) 55, 83, 125, 961, 1081, 1111
Eco57I CTGAAG 3 cut(s) 211, 285, 782
Eco88I CYCGRG 3 cut(s) 396, 884, 1152
EcoO109I RGGNCCY 1 cut(s) 55
EcoT14I CCWWGG 3 cut(s) 969, 1057, 1119
ErhI CCWWGG 3 cut(s) 969, 1057, 1119
FalI AAGNNNNNCTT 2 cut(s) 326, 358
FaqI GGGAC 2 cut(s) 232, 806
FauNDI CATATG 1 cut(s) 364
Fnu4HI GCNGC 5 cut(s) 5, 94, 393, 814, 1125
FokI GGATG 2 cut(s) 857, 862
Fsp4HI GCNGC 5 cut(s) 5, 94, 393, 814, 1125
GluI GCNGC 5 cut(s) 5, 94, 393, 814, 1125
GsuI CTGGAG 2 cut(s) 375, 1151
HaeIII GGCC 4 cut(s) 305, 674, 1063, 1095
HapII CCGG 4 cut(s) 86, 675, 1007, 1092
HincII GTYRAC 2 cut(s) 75, 205
HindII GTYRAC 2 cut(s) 75, 205
HinfI GANTC 8 cut(s) 35, 359, 399, 429, 776, 894, 1150, 1169
HpaII CCGG 4 cut(s) 86, 675, 1007, 1092
HphI GGTGA 1 cut(s) 1190
Hpy166II GTNNAC 6 cut(s) 75, 205, 403, 844, 1149, 1181
Hpy188I TCNGA 6 cut(s) 265, 701, 987, 1036, 1107, 1203
Hpy188III TCNNGA 5 cut(s) 754, 884, 954, 1007, 1051
Hpy8I GTNNAC 6 cut(s) 75, 205, 403, 844, 1149, 1181
HpyAV CCTTC 5 cut(s) 46, 316, 419, 439, 664
HpyCH4III ACNGT 3 cut(s) 613, 823, 993
HpyCH4IV ACGT 1 cut(s) 247
HpyCH4V TGCA 8 cut(s) 93, 482, 621, 642, 813, 914, 936, 1127
HpyF10VI GCNNNNNNNGC 3 cut(s) 311, 392, 1172
HpyF3I CTNAG 3 cut(s) 324, 1067, 1200
HpySE526I ACGT 1 cut(s) 247
Kpn2I TCCGGA 1 cut(s) 1006
KroI GCCGGC 1 cut(s) 1091
KroNI GCCGGC 1 cut(s) 1093
Kzo9I GATC 3 cut(s) 475, 925, 1157
LmnI GCTCC 1 cut(s) 717
Lsp1109I GCAGC 4 cut(s) 105, 379, 825, 1111
LweI GCATC 4 cut(s) 194, 669, 879, 1078
MaeII ACGT 1 cut(s) 247
MaeIII GTNAC 7 cut(s) 150, 239, 248, 257, 777, 947, 997
MalI GATC 3 cut(s) 477, 927, 1159
MboI GATC 3 cut(s) 475, 925, 1157
MboII GAAGA 5 cut(s) 19, 22, 185, 278, 872
MfeI CAATTG 2 cut(s) 899, 1161
MhlI GDGCHC 1 cut(s) 321
MluCI AATT 7 cut(s) 101, 439, 685, 749, 800, 899, 1161
MlyI GAGTC 5 cut(s) 44, 368, 408, 785, 1144
MmeI TCCRAC 1 cut(s) 495
MnlI CCTC 7 cut(s) 706, 846, 869, 880, 1074, 1134, 1137
MroI TCCGGA 1 cut(s) 1006
MroNI GCCGGC 1 cut(s) 1091
MseI TTAA 3 cut(s) 527, 555, 1077
MslI CAYNNNNRTG 2 cut(s) 157, 839
MspCI CTTAAG 1 cut(s) 1076
MspI CCGG 4 cut(s) 86, 675, 1007, 1092
MspR9I CCNGG 1 cut(s) 87
MunI CAATTG 2 cut(s) 899, 1161
MwoI GCNNNNNNNGC 3 cut(s) 311, 392, 1172
NaeI GCCGGC 1 cut(s) 1093
NciI CCSGG 1 cut(s) 87
NcoI CCATGG 2 cut(s) 969, 1057
NdeI CATATG 1 cut(s) 364
NdeII GATC 3 cut(s) 475, 925, 1157
NgoMIV GCCGGC 1 cut(s) 1091
NlaIV GGNNCC 5 cut(s) 57, 719, 857, 962, 1104
NmeAIII GCCGAG 1 cut(s) 340
NmuCI GTSAC 5 cut(s) 150, 239, 248, 257, 777
OliI CACNNNNGTG 1 cut(s) 157
PaeR7I CTCGAG 2 cut(s) 396, 884
PaqCI CACCTGC 1 cut(s) 1166
PceI AGGCCT 1 cut(s) 305
PcsI WCGNNNNNNNCGW 1 cut(s) 253
PdiI GCCGGC 1 cut(s) 1093
PfeI GAWTC 3 cut(s) 429, 894, 1169
PflMI CCANNNNNTGG 2 cut(s) 970, 975
PkrI GCNGC 5 cut(s) 6, 95, 394, 815, 1126
PleI GAGTC 5 cut(s) 43, 367, 407, 784, 1144
PpsI GAGTC 5 cut(s) 43, 367, 407, 784, 1144
PpuMI RGGWCCY 1 cut(s) 55
PshAI GACNNNNGTC 1 cut(s) 255
Psp5II RGGWCCY 1 cut(s) 55
PspN4I GGNNCC 5 cut(s) 57, 719, 857, 962, 1104
PspPI GGNCC 7 cut(s) 55, 83, 125, 961, 1061, 1081, 1111
PspPPI RGGWCCY 1 cut(s) 55
PspXI VCTCGAGB 1 cut(s) 396
PstI CTGCAG 1 cut(s) 484
PstNI CAGNNNCTG 1 cut(s) 539
RsaI GTAC 1 cut(s) 610
RsaNI GTAC 1 cut(s) 609
RseI CAYNNNNRTG 2 cut(s) 157, 839
Rsr2I CGGWCCG 1 cut(s) 83
RsrII CGGWCCG 1 cut(s) 83
SaqAI TTAA 3 cut(s) 527, 555, 1077
SatI GCNGC 5 cut(s) 5, 94, 393, 814, 1125
Sau3AI GATC 3 cut(s) 475, 925, 1157
Sau96I GGNCC 7 cut(s) 55, 83, 125, 961, 1061, 1081, 1111
SchI GAGTC 5 cut(s) 44, 368, 408, 785, 1144
ScrFI CCNGG 1 cut(s) 87
SduI GDGCHC 1 cut(s) 321
SfaNI GCATC 4 cut(s) 194, 669, 879, 1078
SfcI CTRYAG 2 cut(s) 480, 819
Sfr274I CTCGAG 2 cut(s) 396, 884
SinI GGWCC 6 cut(s) 55, 83, 125, 961, 1081, 1111
SlaI CTCGAG 2 cut(s) 396, 884
SmiMI CAYNNNNRTG 2 cut(s) 157, 839
SmlI CTYRAG 5 cut(s) 396, 884, 952, 1049, 1076
SmoI CTYRAG 5 cut(s) 396, 884, 952, 1049, 1076
Sse9I AATT 7 cut(s) 101, 439, 685, 749, 800, 899, 1161
SseBI AGGCCT 1 cut(s) 305
SsiI CCGC 3 cut(s) 5, 81, 1173
StuI AGGCCT 1 cut(s) 305
StyD4I CCNGG 1 cut(s) 85
StyI CCWWGG 3 cut(s) 969, 1057, 1119
TaaI ACNGT 3 cut(s) 613, 823, 993
TaiI ACGT 1 cut(s) 250
TaqI TCGA 6 cut(s) 33, 397, 798, 861, 885, 1026
TasI AATT 7 cut(s) 101, 439, 685, 749, 800, 899, 1161
TauI GCSGC 1 cut(s) 7
TfiI GAWTC 3 cut(s) 429, 894, 1169
Tru1I TTAA 3 cut(s) 527, 555, 1077
Tru9I TTAA 3 cut(s) 527, 555, 1077
TscAI CASTG 4 cut(s) 103, 607, 828, 1134
TseFI GTSAC 5 cut(s) 150, 239, 248, 257, 777
TseI GCWGC 4 cut(s) 93, 392, 813, 1124
Tsp45I GTSAC 5 cut(s) 150, 239, 248, 257, 777
TspDTI ATGAA 4 cut(s) 337, 618, 798, 843
TspGWI ACGGA 1 cut(s) 906
TspRI CASTG 4 cut(s) 103, 607, 828, 1134
Van91I CCANNNNNTGG 2 cut(s) 970, 975
Vha464I CTTAAG 1 cut(s) 1076
VpaK11BI GGWCC 6 cut(s) 55, 83, 125, 961, 1081, 1111
XapI RAATTY 3 cut(s) 439, 749, 800
XcmI CCANNNNNNNNNTGG 1 cut(s) 1126
XhoI CTCGAG 2 cut(s) 396, 884
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.