Rh1DG301000

Belongs to the peptidase S10 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0007698
Physical Location & Seq
Forward (+)
52790363 .. 52794369
4007 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG301000.1

Sequence Viewer

Length: 1074 bp
ATGTTCCTTTGCAAGAGATGTAAAATTGCTCCTTCCTTTCTTTTGCAGGCATCTAATCTGTTGTATGTCGACCAGCCTATTGGTACTGGATTCAGTTATAGTTCTGACAGACGTGATATTCGTCACTCTGAAGATGGTGTTAGTAACGACCTGTATGACTTCTTACAGGCCTTCTTTGCTGAGCATCCTGAGTTGGCAAAGAATGACTTTTACATAACTGGAGAATCATATGCCGGGCACTATATTCCAGCTTTTGCTGCTCGAGTTCACCAAGGAAACAAAGCTAAAGAAGGAATTCATATAAATCTAAAGGGATTTGCCATTGGCAATGGGCTCACTGATCCTGCAATCCAGTATAAAGCATACACAGATTATGCATTGGACAATGGAGTAATCTCGAAAACTGACTACAACCATATTAACAAAGTTCTTCCTGTTTGCGAAATGGCTATAAAGCTGTGTGGTACTGATGGTACAGTCTCTTGCATGGCTTCATATTTTGTGTGCAATACCATATTCAGTAGCATCATCGCGCGAGCTGGAGATGTTAATTACTATGACATCAGAAAGAAGTGTGAGGGGAGCCTCTGCTATGACTTCTCAAACATGGAAAAATTCTTGAAGCAGAAATCTGTTAAACAGGCACTTGGAGTTGGGGACATAGATTTTGTGTCATGTAGCCCTACGGTATATCAAGCTATGCTCGTGGACTGGATGAGGAACCTTGAAGTGGGCATTCCTACTCTTCTTGAGGATGGAATTCAGCTGCTTGTGTATGCCGGAGAGTATGATTTAATCTGCAACTGGCTTGGTAACTCAAGATGGGTCCATGCCATGGAATGGTCTGGTCAGAACAAGTTTGTGAATTCTCCGGAAGTTCCTTTTGTTGTCGATGATTCCGAGGCAGGAGTTCTTAAGAACTATGGGCCTCTTAGTTTCCTGAAGGTCCATGATGCGGGTCACATGGTTCCGATGGACCAGCCCAAGGCCGCATTGGCAATGTTGAAGAGGTGGACTCAGGGCTCCCTTTCTGAAGCCACGGCTGAACCTGAGAATCTGGTTGCTCAGATGTGA

Protein Analysis

357

Amino Acids

39.76

Weight (kDa)

5.38

Isoelectric Point (pI)

39.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S10 PF00450 16 - 341 4.7e-93 Serine carboxypeptidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 835, 840
AccI GTMKAC 1 cut(s) 69
AccII CGCG 2 cut(s) 533, 535
AccIII TCCGGA 1 cut(s) 871
AciI CCGC 2 cut(s) 956, 990
AclWI GGATC 1 cut(s) 335
AcsI RAATTY 4 cut(s) 294, 614, 759, 865
AcuI CTGAAG 3 cut(s) 150, 962, 1053
AfaI GTAC 3 cut(s) 85, 466, 475
AfiI CCNNNNNNNGG 5 cut(s) 730, 835, 840, 955, 985
AflII CTTAAG 1 cut(s) 914
AgsI TTSAA 3 cut(s) 622, 728, 1006
AjiI CACGTC 1 cut(s) 113
AluBI AGCT 6 cut(s) 251, 284, 457, 539, 698, 766
AluI AGCT 6 cut(s) 251, 284, 457, 539, 698, 766
Alw26I GTCTC 1 cut(s) 484
AlwI GGATC 1 cut(s) 335
Ama87I CYCGRG 1 cut(s) 261
Aor13HI TCCGGA 1 cut(s) 871
AoxI GGCC 3 cut(s) 168, 926, 987
ApeKI GCWGC 2 cut(s) 257, 766
ApoI RAATTY 4 cut(s) 294, 614, 759, 865
ArsI GACNNNNNNTTYG 4 cut(s) 102, 134, 650, 682
Asp700I GAANNNNTTC 1 cut(s) 294
AspLEI GCGC 1 cut(s) 535
AspS9I GGNCC 4 cut(s) 826, 926, 946, 976
AsuC2I CCSGG 1 cut(s) 235
AsuHPI GGTGA 1 cut(s) 260
AvaI CYCGRG 1 cut(s) 261
AvaII GGWCC 3 cut(s) 826, 946, 976
BaeGI GKGCMC 1 cut(s) 240
BanII GRGCYC 2 cut(s) 336, 1025
BauI CACGAG 1 cut(s) 704
BbvI GCAGC 2 cut(s) 244, 753
BccI CCATC 5 cut(s) 128, 464, 749, 816, 967
BceAI ACGGC 1 cut(s) 1056
BcnI CCSGG 1 cut(s) 235
BcoDI GTCTC 1 cut(s) 484
BfrI CTTAAG 1 cut(s) 914
BglI GCCNNNNNGGC 1 cut(s) 995
BisI GCNGC 3 cut(s) 258, 767, 990
BlpI GCTNAGC 1 cut(s) 180
BlsI GCNGC 3 cut(s) 259, 768, 991
Bme1390I CCNGG 1 cut(s) 235
Bme18I GGWCC 3 cut(s) 826, 946, 976
BmeT110I CYCGRG 1 cut(s) 261
BmgBI CACGTC 1 cut(s) 113
BmgT120I GGNCC 4 cut(s) 826, 926, 946, 976
BmiI GGNNCC 5 cut(s) 584, 722, 827, 969, 1024
BmrFI CCNGG 1 cut(s) 235
BmsI GCATC 4 cut(s) 59, 193, 534, 943
BplI GAGNNNNNCTC 2 cut(s) 1000, 1032
BpmI CTGGAG 2 cut(s) 240, 561
Bpu1102I GCTNAGC 1 cut(s) 180
BpuEI CTTGAG 2 cut(s) 770, 802
BpuMI CCSGG 1 cut(s) 235
BsaJI CCNNGG 5 cut(s) 271, 834, 900, 984, 1038
BsaWI WCCGGW 1 cut(s) 871
Bsc4I CCNNNNNNNGG 5 cut(s) 730, 835, 840, 955, 985
Bse1I ACTGG 5 cut(s) 91, 223, 352, 716, 809
Bse3DI GCAATG 2 cut(s) 334, 1005
BseAI TCCGGA 1 cut(s) 871
BseDI CCNNGG 5 cut(s) 271, 834, 900, 984, 1038
BseGI GGATG 3 cut(s) 184, 720, 760
BseLI CCNNNNNNNGG 5 cut(s) 730, 835, 840, 955, 985
BseMI GCAATG 2 cut(s) 334, 1005
BseMII CTCAG 4 cut(s) 171, 180, 1031, 1041
BseNI ACTGG 5 cut(s) 91, 223, 352, 716, 809
BseSI GKGCMC 1 cut(s) 240
BseXI GCAGC 2 cut(s) 244, 753
Bsh1236I CGCG 2 cut(s) 533, 535
BshFI GGCC 3 cut(s) 170, 928, 989
BsiHKCI CYCGRG 1 cut(s) 261
BsiSI CCGG 3 cut(s) 234, 780, 872
BslFI GGGAC 1 cut(s) 671
BslI CCNNNNNNNGG 5 cut(s) 730, 835, 840, 955, 985
BsmAI GTCTC 1 cut(s) 484
BsmFI GGGAC 1 cut(s) 671
BsmI GAATGC 1 cut(s) 735
BsnI GGCC 3 cut(s) 170, 928, 989
BsoBI CYCGRG 1 cut(s) 261
Bsp1286I GDGCHC 3 cut(s) 240, 336, 1025
Bsp13I TCCGGA 1 cut(s) 871
Bsp143I GATC 1 cut(s) 340
Bsp1720I GCTNAGC 1 cut(s) 180
Bsp19I CCATGG 1 cut(s) 834
BspACI CCGC 2 cut(s) 956, 990
BspANI GGCC 3 cut(s) 170, 928, 989
BspCNI CTCAG 4 cut(s) 172, 181, 1030, 1042
BspEI TCCGGA 1 cut(s) 871
BspFNI CGCG 2 cut(s) 533, 535
BspLI GGNNCC 5 cut(s) 584, 722, 827, 969, 1024
BspPI GGATC 1 cut(s) 335
BspTI CTTAAG 1 cut(s) 914
BsrDI GCAATG 2 cut(s) 334, 1005
BsrI ACTGG 5 cut(s) 91, 223, 352, 716, 809
BssECI CCNNGG 5 cut(s) 271, 834, 900, 984, 1038
BssMI GATC 1 cut(s) 340
BssSI CACGAG 1 cut(s) 704
BssT1I CCWWGG 3 cut(s) 271, 834, 984
Bst2BI CACGAG 1 cut(s) 704
Bst4CI ACNGT 2 cut(s) 478, 688
Bst6I CTCTTC 2 cut(s) 750, 1001
BstAFI CTTAAG 1 cut(s) 914
BstC8I GCNNGC 2 cut(s) 48, 537
BstDEI CTNAG 6 cut(s) 180, 189, 932, 1017, 1050, 1065
BstDSI CCRYGG 2 cut(s) 834, 1038
BstF5I GGATG 3 cut(s) 184, 720, 760
BstFNI CGCG 2 cut(s) 533, 535
BstHHI GCGC 1 cut(s) 535
BstKTI GATC 1 cut(s) 343
BstMAI GTCTC 1 cut(s) 484
BstMBI GATC 1 cut(s) 340
BstMWI GCNNNNNNNGC 3 cut(s) 176, 257, 995
BstSCI CCNGG 1 cut(s) 233
BstSLI GKGCMC 1 cut(s) 240
BstUI CGCG 2 cut(s) 533, 535
BstV1I GCAGC 2 cut(s) 244, 753
BstXI CCANNNNNNTGG 1 cut(s) 80
BsuRI GGCC 3 cut(s) 170, 928, 989
BtgI CCRYGG 2 cut(s) 834, 1038
BtgZI GCGATG 1 cut(s) 514
BtrI CACGTC 1 cut(s) 113
BtsCI GGATG 3 cut(s) 184, 720, 760
BtsIMutI CAGTG 1 cut(s) 336
Cac8I GCNNGC 2 cut(s) 48, 537
CfoI GCGC 1 cut(s) 535
Cfr13I GGNCC 4 cut(s) 826, 926, 946, 976
Csp6I GTAC 3 cut(s) 84, 465, 474
CviAII CATG 7 cut(s) 487, 607, 675, 830, 835, 950, 964
CviQI GTAC 3 cut(s) 84, 465, 474
DdeI CTNAG 6 cut(s) 180, 189, 932, 1017, 1050, 1065
DpnI GATC 1 cut(s) 342
DpnII GATC 1 cut(s) 340
Eam1104I CTCTTC 2 cut(s) 750, 1001
EarI CTCTTC 2 cut(s) 750, 1001
Eco130I CCWWGG 3 cut(s) 271, 834, 984
Eco147I AGGCCT 1 cut(s) 170
Eco24I GRGCYC 2 cut(s) 336, 1025
Eco47I GGWCC 3 cut(s) 826, 946, 976
Eco57I CTGAAG 3 cut(s) 150, 962, 1053
Eco88I CYCGRG 1 cut(s) 261
EcoRI GAATTC 3 cut(s) 294, 759, 865
EcoT14I CCWWGG 3 cut(s) 271, 834, 984
EcoT22I ATGCAT 1 cut(s) 379
EcoT38I GRGCYC 2 cut(s) 336, 1025
ErhI CCWWGG 3 cut(s) 271, 834, 984
FaeI CATG 7 cut(s) 490, 610, 678, 833, 838, 953, 967
FalI AAGNNNNNCTT 2 cut(s) 191, 223
FaqI GGGAC 1 cut(s) 671
FatI CATG 7 cut(s) 486, 606, 674, 829, 834, 949, 963
FauI CCCGC 1 cut(s) 949
FauNDI CATATG 1 cut(s) 229
FblI GTMKAC 1 cut(s) 69
Fnu4HI GCNGC 3 cut(s) 258, 767, 990
FokI GGATG 3 cut(s) 171, 727, 767
FriOI GRGCYC 2 cut(s) 336, 1025
Fsp4HI GCNGC 3 cut(s) 258, 767, 990
GlaI GCGC 1 cut(s) 534
GluI GCNGC 3 cut(s) 258, 767, 990
GsuI CTGGAG 2 cut(s) 240, 561
HaeIII GGCC 3 cut(s) 170, 928, 989
HapII CCGG 3 cut(s) 234, 780, 872
HhaI GCGC 1 cut(s) 535
Hin1II CATG 7 cut(s) 490, 610, 678, 833, 838, 953, 967
Hin6I GCGC 1 cut(s) 533
HinP1I GCGC 1 cut(s) 533
HincII GTYRAC 1 cut(s) 70
HindII GTYRAC 1 cut(s) 70
HinfI GANTC 5 cut(s) 90, 224, 896, 1015, 1054
HpaII CCGG 3 cut(s) 234, 780, 872
HphI GGTGA 1 cut(s) 260
Hpy166II GTNNAC 4 cut(s) 70, 268, 709, 1014
Hpy188I TCNGA 8 cut(s) 106, 130, 566, 852, 901, 972, 1033, 1068
Hpy188III TCNNGA 7 cut(s) 188, 397, 619, 749, 819, 872, 940
Hpy8I GTNNAC 4 cut(s) 70, 268, 709, 1014
HpyAV CCTTC 4 cut(s) 42, 181, 284, 937
HpyCH4III ACNGT 2 cut(s) 478, 688
HpyCH4IV ACGT 1 cut(s) 112
HpyCH4V TGCA 7 cut(s) 12, 46, 347, 377, 486, 507, 801
HpyF10VI GCNNNNNNNGC 3 cut(s) 176, 257, 995
HpyF3I CTNAG 6 cut(s) 180, 189, 932, 1017, 1050, 1065
HpySE526I ACGT 1 cut(s) 112
Hsp92II CATG 7 cut(s) 490, 610, 678, 833, 838, 953, 967
HspAI GCGC 1 cut(s) 533
Kpn2I TCCGGA 1 cut(s) 871
Kzo9I GATC 1 cut(s) 340
LmnI GCTCC 3 cut(s) 34, 582, 1028
Lsp1109I GCAGC 2 cut(s) 244, 753
LweI GCATC 4 cut(s) 59, 193, 534, 943
MaeII ACGT 1 cut(s) 112
MaeIII GTNAC 4 cut(s) 122, 143, 812, 959
MalI GATC 1 cut(s) 342
MboI GATC 1 cut(s) 340
MboII GAAGA 4 cut(s) 143, 422, 737, 1018
MhlI GDGCHC 3 cut(s) 240, 336, 1025
MluCI AATT 6 cut(s) 24, 294, 550, 614, 759, 865
MlyI GAGTC 1 cut(s) 1009
MnlI CCTC 7 cut(s) 571, 596, 711, 745, 895, 939, 1002
Mph1103I ATGCAT 1 cut(s) 379
MroI TCCGGA 1 cut(s) 871
MroXI GAANNNNTTC 1 cut(s) 294
MseI TTAA 5 cut(s) 420, 549, 636, 794, 915
MspA1I CMGCKG 1 cut(s) 766
MspCI CTTAAG 1 cut(s) 914
MspI CCGG 3 cut(s) 234, 780, 872
MspR9I CCNGG 1 cut(s) 235
Mva1269I GAATGC 1 cut(s) 735
MvnI CGCG 2 cut(s) 533, 535
MwoI GCNNNNNNNGC 3 cut(s) 176, 257, 995
NciI CCSGG 1 cut(s) 235
NcoI CCATGG 1 cut(s) 834
NdeI CATATG 1 cut(s) 229
NdeII GATC 1 cut(s) 340
NlaIII CATG 7 cut(s) 490, 610, 678, 833, 838, 953, 967
NlaIV GGNNCC 5 cut(s) 584, 722, 827, 969, 1024
NmuCI GTSAC 2 cut(s) 122, 959
NsiI ATGCAT 1 cut(s) 379
PaeR7I CTCGAG 1 cut(s) 261
PceI AGGCCT 1 cut(s) 170
PcsI WCGNNNNNNNCGW 2 cut(s) 118, 897
PctI GAATGC 1 cut(s) 735
PdmI GAANNNNTTC 1 cut(s) 294
PfeI GAWTC 4 cut(s) 90, 224, 896, 1054
PflMI CCANNNNNTGG 2 cut(s) 835, 840
PkrI GCNGC 3 cut(s) 259, 768, 991
PleI GAGTC 1 cut(s) 1009
PpsI GAGTC 1 cut(s) 1009
PspN4I GGNNCC 5 cut(s) 584, 722, 827, 969, 1024
PspPI GGNCC 4 cut(s) 826, 926, 946, 976
PspXI VCTCGAGB 1 cut(s) 261
PvuII CAGCTG 1 cut(s) 766
RsaI GTAC 3 cut(s) 85, 466, 475
RsaNI GTAC 3 cut(s) 84, 465, 474
SalI GTCGAC 1 cut(s) 68
SaqAI TTAA 5 cut(s) 420, 549, 636, 794, 915
SatI GCNGC 3 cut(s) 258, 767, 990
Sau3AI GATC 1 cut(s) 340
Sau96I GGNCC 4 cut(s) 826, 926, 946, 976
SchI GAGTC 1 cut(s) 1009
ScrFI CCNGG 1 cut(s) 235
SduI GDGCHC 3 cut(s) 240, 336, 1025
SfaNI GCATC 4 cut(s) 59, 193, 534, 943
Sfr274I CTCGAG 1 cut(s) 261
SinI GGWCC 3 cut(s) 826, 946, 976
SlaI CTCGAG 1 cut(s) 261
SmlI CTYRAG 4 cut(s) 261, 749, 817, 914
SmoI CTYRAG 4 cut(s) 261, 749, 817, 914
Sse9I AATT 6 cut(s) 24, 294, 550, 614, 759, 865
SseBI AGGCCT 1 cut(s) 170
SsiI CCGC 2 cut(s) 956, 990
StuI AGGCCT 1 cut(s) 170
StyD4I CCNGG 1 cut(s) 233
StyI CCWWGG 3 cut(s) 271, 834, 984
TaaI ACNGT 2 cut(s) 478, 688
TaiI ACGT 1 cut(s) 115
TaqI TCGA 4 cut(s) 69, 262, 398, 891
TasI AATT 6 cut(s) 24, 294, 550, 614, 759, 865
TauI GCSGC 1 cut(s) 992
TfiI GAWTC 4 cut(s) 90, 224, 896, 1054
Tru1I TTAA 5 cut(s) 420, 549, 636, 794, 915
Tru9I TTAA 5 cut(s) 420, 549, 636, 794, 915
TscAI CASTG 1 cut(s) 343
TseFI GTSAC 2 cut(s) 122, 959
TseI GCWGC 2 cut(s) 257, 766
Tsp45I GTSAC 2 cut(s) 122, 959
TspDTI ATGAA 2 cut(s) 287, 483
TspRI CASTG 1 cut(s) 343
Van91I CCANNNNNTGG 2 cut(s) 835, 840
Vha464I CTTAAG 1 cut(s) 914
VpaK11BI GGWCC 3 cut(s) 826, 946, 976
XapI RAATTY 4 cut(s) 294, 614, 759, 865
XcmI CCANNNNNNNNNTGG 1 cut(s) 991
XhoI CTCGAG 1 cut(s) 261
XmiI GTMKAC 1 cut(s) 69
XmnI GAANNNNTTC 1 cut(s) 294
Zsp2I ATGCAT 1 cut(s) 379
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.