pycom01g14090

Nuclear transcription factor Y subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0003710
Physical Location & Seq
Reverse (-)
14361322 .. 14363786
2465 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 537 bp
ATGGCGGAGGCACCGACGAGTCCGGCCGGCGGGAGCCACGAGAGCGGCGGCGAGCAGAGCCCCCAGGGAGGAGGAGGAGGAGGCGGCGGCGGCGTCAGGGAGCAGGATAGGTATTTGCCAATCGCCAACATCAGTCGCATCATGAAGAAGGCTCTGCCGACTAACGGCAAGATCGCCAAGGACGCCAAGGACACCGTCCAGGAATGCGTCTCCGAATTCATCAGCTTCGTCACCAGCGAGGCCAGCGATAAGTGCCAGAAGGAGAAGCGGAAGACCATCAACGGCGACGATTTGTTGTGGGCGATGGCCACGTTAGGGTTTGAGGACTATATCGAGCCCCTCAGGATTTACTTGGCTAGGTACAGGGAGGGTGATGCAAAGGGGTCTGCTAGGGGTGGAGATGGTTCTTCAAAAGGGAATGCTGTTCCAGCAATGCCTGGCCCAAGTGCACAGTTTGTTCATCAGGGAGCATTGAATTATGGCAACCCTCAAGAGGATTATTATGTGCCATTGAACAATATGGGTAATGGAAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko04612 ko05152 ko05166 map04612 map05152 map05166
Pfam Domains
Protein Families
CBFD_NFYB_HMF

Protein Analysis

179

Amino Acids

18.74

Weight (kDa)

5.45

Isoelectric Point (pI)

51.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CBFD_NFYB_HMF PF00808 38 - 102 5e-28 Histone-like transcription factor (CBF/NF-Y) and archaeal histone
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 10
AccBSI CCGCTC 1 cut(s) 45
AciI CCGC 8 cut(s) 5, 30, 45, 48, 84, 87, 90, 268
AcoI YGGCCR 2 cut(s) 24, 306
AcsI RAATTY 1 cut(s) 215
AcyI GRCGYC 2 cut(s) 93, 183
AfaI GTAC 1 cut(s) 362
AfiI CCNNNNNNNGG 5 cut(s) 29, 68, 164, 315, 493
AgsI TTSAA 3 cut(s) 411, 475, 514
AjnI CCWGG 3 cut(s) 63, 198, 436
AluBI AGCT 1 cut(s) 225
AluI AGCT 1 cut(s) 225
Alw21I GWGCWC 1 cut(s) 451
Alw26I GTCTC 1 cut(s) 214
Alw44I GTGCAC 1 cut(s) 447
AoxI GGCC 4 cut(s) 24, 240, 306, 439
ApaLI GTGCAC 1 cut(s) 447
ApoI RAATTY 1 cut(s) 215
AspS9I GGNCC 1 cut(s) 440
AsuHPI GGTGA 2 cut(s) 223, 383
AxyI CCTNAGG 1 cut(s) 341
BaeGI GKGCMC 1 cut(s) 451
BalI TGGCCA 1 cut(s) 308
BanI GGYRCC 1 cut(s) 10
BanII GRGCYC 2 cut(s) 62, 339
BauI CACGAG 1 cut(s) 38
BbsI GAAGAC 1 cut(s) 278
Bbv12I GWGCWC 1 cut(s) 451
BccI CCATC 3 cut(s) 284, 298, 395
BceAI ACGGC 2 cut(s) 181, 298
BciT130I CCWGG 3 cut(s) 65, 200, 438
BcoDI GTCTC 1 cut(s) 214
BfaI CTAG 2 cut(s) 357, 390
BisI GCNGC 5 cut(s) 46, 49, 85, 88, 91
BlsI GCNGC 5 cut(s) 47, 50, 86, 89, 92
Bme1390I CCNGG 3 cut(s) 65, 200, 438
BmgT120I GGNCC 1 cut(s) 440
BmiI GGNNCC 2 cut(s) 12, 35
BmrFI CCNGG 3 cut(s) 65, 200, 438
BmsI GCATC 2 cut(s) 147, 364
BpiI GAAGAC 1 cut(s) 278
BpuEI CTTGAG 1 cut(s) 474
BsaHI GRCGYC 2 cut(s) 93, 183
BsaJI CCNNGG 4 cut(s) 63, 64, 177, 186
Bsc4I CCNNNNNNNGG 5 cut(s) 29, 68, 164, 315, 493
Bse118I RCCGGY 1 cut(s) 26
Bse21I CCTNAGG 1 cut(s) 341
Bse3DI GCAATG 1 cut(s) 438
BseBI CCWGG 3 cut(s) 65, 200, 438
BseDI CCNNGG 4 cut(s) 63, 64, 177, 186
BseLI CCNNNNNNNGG 5 cut(s) 29, 68, 164, 315, 493
BseMI GCAATG 1 cut(s) 438
BseMII CTCAG 1 cut(s) 355
BseRI GAGGAG 4 cut(s) 84, 87, 90, 93
BseSI GKGCMC 1 cut(s) 451
BseX3I CGGCCG 1 cut(s) 24
Bsh1285I CGRYCG 1 cut(s) 27
BshFI GGCC 4 cut(s) 26, 242, 308, 441
BshNI GGYRCC 1 cut(s) 10
BsiEI CGRYCG 1 cut(s) 27
BsiHKAI GWGCWC 1 cut(s) 451
BsiSI CCGG 2 cut(s) 23, 27
BslI CCNNNNNNNGG 5 cut(s) 29, 68, 164, 315, 493
BsmAI GTCTC 1 cut(s) 214
BsmBI CGTCTC 1 cut(s) 214
BsmI GAATGC 2 cut(s) 209, 424
BsnI GGCC 4 cut(s) 26, 242, 308, 441
Bsp1286I GDGCHC 3 cut(s) 62, 339, 451
Bsp143I GATC 1 cut(s) 171
BspACI CCGC 8 cut(s) 5, 30, 45, 48, 84, 87, 90, 268
BspANI GGCC 4 cut(s) 26, 242, 308, 441
BspCNI CTCAG 1 cut(s) 354
BspHI TCATGA 1 cut(s) 141
BspLI GGNNCC 2 cut(s) 12, 35
BspT107I GGYRCC 1 cut(s) 10
BsrBI CCGCTC 1 cut(s) 45
BsrDI GCAATG 1 cut(s) 438
BsrFI RCCGGY 1 cut(s) 26
BssAI RCCGGY 1 cut(s) 26
BssECI CCNNGG 4 cut(s) 63, 64, 177, 186
BssMI GATC 1 cut(s) 171
BssNI GRCGYC 2 cut(s) 93, 183
BssSI CACGAG 1 cut(s) 38
BssT1I CCWWGG 2 cut(s) 177, 186
Bst2BI CACGAG 1 cut(s) 38
Bst2UI CCWGG 3 cut(s) 65, 200, 438
Bst4CI ACNGT 2 cut(s) 196, 453
BstACI GRCGYC 2 cut(s) 93, 183
BstC8I GCNNGC 3 cut(s) 28, 53, 244
BstDEI CTNAG 1 cut(s) 341
BstKTI GATC 1 cut(s) 174
BstMAI GTCTC 1 cut(s) 214
BstMBI GATC 1 cut(s) 171
BstMCI CGRYCG 1 cut(s) 27
BstMWI GCNNNNNNNGC 7 cut(s) 42, 57, 90, 182, 243, 252, 428
BstNI CCWGG 3 cut(s) 65, 200, 438
BstSCI CCNGG 3 cut(s) 63, 198, 436
BstSLI GKGCMC 1 cut(s) 451
BstV2I GAAGAC 1 cut(s) 278
BstZI CGGCCG 1 cut(s) 24
Bsu36I CCTNAGG 1 cut(s) 341
BsuRI GGCC 4 cut(s) 26, 242, 308, 441
BtgZI GCGATG 1 cut(s) 317
Cac8I GCNNGC 3 cut(s) 28, 53, 244
CciI TCATGA 1 cut(s) 141
Cfr10I RCCGGY 1 cut(s) 26
Cfr13I GGNCC 1 cut(s) 440
CseI GACGC 3 cut(s) 82, 191, 196
Csp6I GTAC 1 cut(s) 361
CviAII CATG 1 cut(s) 142
CviQI GTAC 1 cut(s) 361
DdeI CTNAG 1 cut(s) 341
DpnI GATC 1 cut(s) 173
DpnII GATC 1 cut(s) 171
EaeI YGGCCR 2 cut(s) 24, 306
EagI CGGCCG 1 cut(s) 24
EciI GGCGGA 1 cut(s) 20
EclXI CGGCCG 1 cut(s) 24
Eco130I CCWWGG 2 cut(s) 177, 186
Eco24I GRGCYC 2 cut(s) 62, 339
Eco52I CGGCCG 1 cut(s) 24
Eco81I CCTNAGG 1 cut(s) 341
EcoRI GAATTC 1 cut(s) 215
EcoRII CCWGG 3 cut(s) 63, 198, 436
EcoT14I CCWWGG 2 cut(s) 177, 186
EcoT38I GRGCYC 2 cut(s) 62, 339
ErhI CCWWGG 2 cut(s) 177, 186
Esp3I CGTCTC 1 cut(s) 214
FaeI CATG 1 cut(s) 145
FaiI YATR 5 cut(s) 143, 330, 480, 504, 521
FatI CATG 1 cut(s) 141
FauI CCCGC 1 cut(s) 23
Fnu4HI GCNGC 5 cut(s) 46, 49, 85, 88, 91
FriOI GRGCYC 2 cut(s) 62, 339
Fsp4HI GCNGC 5 cut(s) 46, 49, 85, 88, 91
FspBI CTAG 2 cut(s) 357, 390
GluI GCNGC 5 cut(s) 46, 49, 85, 88, 91
HaeIII GGCC 4 cut(s) 26, 242, 308, 441
HapII CCGG 2 cut(s) 23, 27
HgaI GACGC 3 cut(s) 82, 191, 196
Hin1I GRCGYC 2 cut(s) 93, 183
Hin1II CATG 1 cut(s) 145
HinfI GANTC 1 cut(s) 19
HpaII CCGG 2 cut(s) 23, 27
HphI GGTGA 2 cut(s) 223, 383
Hpy166II GTNNAC 1 cut(s) 449
Hpy188I TCNGA 1 cut(s) 214
Hpy188III TCNNGA 3 cut(s) 142, 343, 491
Hpy8I GTNNAC 1 cut(s) 449
Hpy99I CGWCG 2 cut(s) 19, 290
HpyAV CCTTC 2 cut(s) 142, 253
HpyCH4III ACNGT 2 cut(s) 196, 453
HpyCH4IV ACGT 1 cut(s) 311
HpyCH4V TGCA 2 cut(s) 377, 449
HpyF10VI GCNNNNNNNGC 7 cut(s) 42, 57, 90, 182, 243, 252, 428
HpyF3I CTNAG 1 cut(s) 341
HpySE526I ACGT 1 cut(s) 311
Hsp92I GRCGYC 2 cut(s) 93, 183
Hsp92II CATG 1 cut(s) 145
KroI GCCGGC 1 cut(s) 26
KroNI GCCGGC 1 cut(s) 28
Kzo9I GATC 1 cut(s) 171
LmnI GCTCC 3 cut(s) 33, 100, 467
LweI GCATC 2 cut(s) 147, 364
MaeI CTAG 2 cut(s) 357, 390
MaeII ACGT 1 cut(s) 311
MaeIII GTNAC 1 cut(s) 229
MalI GATC 1 cut(s) 173
MbiI CCGCTC 1 cut(s) 45
MboI GATC 1 cut(s) 171
MboII GAAGA 3 cut(s) 157, 283, 399
MhlI GDGCHC 3 cut(s) 62, 339, 451
MlsI TGGCCA 1 cut(s) 308
MluCI AATT 2 cut(s) 215, 475
MluNI TGGCCA 1 cut(s) 308
MlyI GAGTC 1 cut(s) 28
Mox20I TGGCCA 1 cut(s) 308
MroNI GCCGGC 1 cut(s) 26
MscI TGGCCA 1 cut(s) 308
Msp20I TGGCCA 1 cut(s) 308
MspI CCGG 2 cut(s) 23, 27
MspR9I CCNGG 3 cut(s) 65, 200, 438
Mva1269I GAATGC 2 cut(s) 209, 424
MvaI CCWGG 3 cut(s) 65, 200, 438
MwoI GCNNNNNNNGC 7 cut(s) 42, 57, 90, 182, 243, 252, 428
NaeI GCCGGC 1 cut(s) 28
NdeII GATC 1 cut(s) 171
NgoMIV GCCGGC 1 cut(s) 26
NlaIII CATG 1 cut(s) 145
NlaIV GGNNCC 2 cut(s) 12, 35
NmuCI GTSAC 1 cut(s) 229
PagI TCATGA 1 cut(s) 141
PasI CCCWGGG 1 cut(s) 64
PcsI WCGNNNNNNNCGW 1 cut(s) 234
PctI GAATGC 2 cut(s) 209, 424
PdiI GCCGGC 1 cut(s) 28
PflFI GACNNNGTC 1 cut(s) 194
PfoI TCCNGGA 1 cut(s) 198
PkrI GCNGC 5 cut(s) 47, 50, 86, 89, 92
PleI GAGTC 1 cut(s) 27
PpsI GAGTC 1 cut(s) 27
Psp6I CCWGG 3 cut(s) 63, 198, 436
PspGI CCWGG 3 cut(s) 63, 198, 436
PspN4I GGNNCC 2 cut(s) 12, 35
PspPI GGNCC 1 cut(s) 440
PsyI GACNNNGTC 1 cut(s) 194
RsaI GTAC 1 cut(s) 362
RsaNI GTAC 1 cut(s) 361
SatI GCNGC 5 cut(s) 46, 49, 85, 88, 91
Sau3AI GATC 1 cut(s) 171
Sau96I GGNCC 1 cut(s) 440
SchI GAGTC 1 cut(s) 28
ScrFI CCNGG 3 cut(s) 65, 200, 438
SduI GDGCHC 3 cut(s) 62, 339, 451
SetI ASST 4 cut(s) 113, 227, 314, 362
SfaNI GCATC 2 cut(s) 147, 364
SmlI CTYRAG 1 cut(s) 489
SmoI CTYRAG 1 cut(s) 489
Sse9I AATT 2 cut(s) 215, 475
SsiI CCGC 8 cut(s) 5, 30, 45, 48, 84, 87, 90, 268
SspMI CTAG 2 cut(s) 357, 390
StyD4I CCNGG 3 cut(s) 63, 198, 436
StyI CCWWGG 2 cut(s) 177, 186
TaaI ACNGT 2 cut(s) 196, 453
TaiI ACGT 1 cut(s) 314
TaqI TCGA 1 cut(s) 333
TasI AATT 2 cut(s) 215, 475
TauI GCSGC 5 cut(s) 48, 51, 87, 90, 93
TseFI GTSAC 1 cut(s) 229
Tsp45I GTSAC 1 cut(s) 229
TspDTI ATGAA 3 cut(s) 158, 208, 449
Tth111I GACNNNGTC 1 cut(s) 194
VneI GTGCAC 1 cut(s) 447
XapI RAATTY 1 cut(s) 215
XspI CTAG 2 cut(s) 357, 390
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.