pycom01g22700

Removes the phosphate from trehalose 6-phosphate to produce free trehalose

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0008155
Physical Location & Seq
Reverse (-)
20194184 .. 20196802
2619 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 828 bp
ATGGGACCCTTTCACCAATTGTTGATGATCGATCGTGCCTTCATGTCTGATGAGATGCGCGCAGCAGTACGGGAAGTTGCCAAGTATTTTCCAACGGCAGTAATTAGTGGAAGGAGTAGAGATAAGGTAAAAGAATTTGTGCAGTTAAGTAATGTATATTATGCTGGAAGCCATGGGATGGATATATTGGTCCCAACAGGGCCACTAAATCCCTGTGATGCCAAGAACCACACAGCAGCCATTGATAAAAAGGAGAGTGACGTTCTCTTTCAACCTGCTAAAAGATTTCTGCCTGCAATCCAAGAGATACGAACACTGTTAGAGGAGCTAACCAGGAAAGTAGAAGGTGCTAGGGTAGAGGACAACAGATTCTGCATTTCTGTTCATTTCCGCAAGGTCCGGGATGAGGATTATGGCATATTAGAAGCAAAGGTGAAGTACGTACTTGAAAACTATCCTGAATTTCATCTCACCTTGGGCAAAAAGGTCTTGGAAGTACGGCCATCCATAGAATGGAACAAAGGTCATGCCTTAGAATATATACTTGACACTCTTGGATTCAGCAGTTCCAGTGACGTCCTCCCGTTGTACATTGGGGACGACCGGACTGATGAAGATGCTTTCAAGATGATACGAAGTAGAGGACTAGGGTTTCCGATAATTGTGTCTTCAACCCCGAAGGATACCAAAGCTTGCTATTCCCTGCACGATCCGTCAGATGTTTTAACTTTCTTGTTGCGGCTTGCAAGGTGGAGAAAAGCGTCTTCTTCTAGCAGGTCACTTGCTCAAATTTGGGGTCCTGGAAATTTACCTAGATCAGTTAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
Trehalose_PPase

Protein Analysis

276

Amino Acids

31.16

Weight (kDa)

8.64

Isoelectric Point (pI)

44.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Trehalose_PPase PF02358 11 - 239 3.5e-51 Trehalose-phosphatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 579
Acc36I ACCTGC 2 cut(s) 283, 765
AccB7I CCANNNNNTGG 2 cut(s) 178, 513
AccII CGCG 1 cut(s) 60
AciI CCGC 2 cut(s) 391, 739
AclWI GGATC 1 cut(s) 704
AcoI YGGCCR 1 cut(s) 500
AcsI RAATTY 4 cut(s) 134, 461, 789, 805
AcyI GRCGYC 1 cut(s) 576
AfaI GTAC 5 cut(s) 69, 440, 444, 498, 590
AfiI CCNNNNNNNGG 3 cut(s) 178, 406, 513
AgsI TTSAA 4 cut(s) 272, 449, 625, 672
AjnI CCWGG 2 cut(s) 332, 799
AluBI AGCT 2 cut(s) 328, 692
AluI AGCT 2 cut(s) 328, 692
AlwI GGATC 1 cut(s) 704
AlwNI CAGNNNCTG 1 cut(s) 372
AoxI GGCC 2 cut(s) 200, 500
ApeKI GCWGC 2 cut(s) 62, 236
ApoI RAATTY 4 cut(s) 134, 461, 789, 805
ArsI GACNNNNNNTTYG 2 cut(s) 781, 813
AspLEI GCGC 2 cut(s) 60, 62
AspS9I GGNCC 5 cut(s) 5, 190, 200, 397, 797
AsuC2I CCSGG 1 cut(s) 401
AsuHPI GGTGA 3 cut(s) 5, 445, 463
AvaII GGWCC 4 cut(s) 5, 190, 397, 797
BbsI GAAGAC 2 cut(s) 660, 756
BbvI GCAGC 2 cut(s) 74, 248
BccI CCATC 2 cut(s) 172, 511
BceAI ACGGC 2 cut(s) 111, 515
BciT130I CCWGG 2 cut(s) 334, 801
BciVI GTATCC 1 cut(s) 676
BcnI CCSGG 1 cut(s) 401
BfaI CTAG 4 cut(s) 351, 647, 771, 813
BfuAI ACCTGC 2 cut(s) 283, 765
BfuI GTATCC 1 cut(s) 676
BisI GCNGC 3 cut(s) 63, 237, 740
BlsI GCNGC 3 cut(s) 64, 238, 741
Bme1390I CCNGG 3 cut(s) 334, 401, 801
Bme18I GGWCC 4 cut(s) 5, 190, 397, 797
BmgT120I GGNCC 5 cut(s) 5, 190, 200, 397, 797
BmiI GGNNCC 4 cut(s) 6, 7, 192, 798
BmrFI CCNGG 3 cut(s) 334, 401, 801
BmsI GCATC 3 cut(s) 45, 208, 607
BpiI GAAGAC 2 cut(s) 660, 756
BpuMI CCSGG 1 cut(s) 401
Bsa29I ATCGAT 1 cut(s) 30
BsaAI YACGTR 1 cut(s) 442
BsaHI GRCGYC 1 cut(s) 576
BsaJI CCNNGG 2 cut(s) 172, 474
BsaWI WCCGGW 1 cut(s) 603
Bsc4I CCNNNNNNNGG 3 cut(s) 178, 406, 513
Bse1I ACTGG 1 cut(s) 570
BseBI CCWGG 2 cut(s) 334, 801
BseCI ATCGAT 1 cut(s) 30
BseDI CCNNGG 2 cut(s) 172, 474
BseGI GGATG 3 cut(s) 183, 409, 503
BseLI CCNNNNNNNGG 3 cut(s) 178, 406, 513
BseNI ACTGG 1 cut(s) 570
BsePI GCGCGC 1 cut(s) 58
BseRI GAGGAG 1 cut(s) 338
BseXI GCAGC 2 cut(s) 74, 248
BsgI GTGCAG 2 cut(s) 161, 689
Bsh1236I CGCG 1 cut(s) 60
Bsh1285I CGRYCG 2 cut(s) 34, 604
BshFI GGCC 2 cut(s) 202, 502
BshVI ATCGAT 1 cut(s) 30
BsiEI CGRYCG 2 cut(s) 34, 604
BsiSI CCGG 2 cut(s) 400, 604
BslFI GGGAC 3 cut(s) 18, 176, 611
BslI CCNNNNNNNGG 3 cut(s) 178, 406, 513
BsmFI GGGAC 3 cut(s) 18, 176, 611
BsnI GGCC 2 cut(s) 202, 502
Bsp1407I TGTACA 1 cut(s) 588
Bsp143I GATC 4 cut(s) 27, 31, 709, 815
Bsp19I CCATGG 1 cut(s) 172
BspACI CCGC 2 cut(s) 391, 739
BspANI GGCC 2 cut(s) 202, 502
BspDI ATCGAT 1 cut(s) 30
BspFNI CGCG 1 cut(s) 60
BspLI GGNNCC 4 cut(s) 6, 7, 192, 798
BspMI ACCTGC 2 cut(s) 283, 765
BspPI GGATC 1 cut(s) 704
BsrGI TGTACA 1 cut(s) 588
BsrI ACTGG 1 cut(s) 570
BssECI CCNNGG 2 cut(s) 172, 474
BssHII GCGCGC 1 cut(s) 58
BssMI GATC 4 cut(s) 27, 31, 709, 815
BssNI GRCGYC 1 cut(s) 576
BssT1I CCWWGG 2 cut(s) 172, 474
Bst2UI CCWGG 2 cut(s) 334, 801
Bst4CI ACNGT 1 cut(s) 318
BstACI GRCGYC 1 cut(s) 576
BstAUI TGTACA 1 cut(s) 588
BstBAI YACGTR 1 cut(s) 442
BstC8I GCNNGC 4 cut(s) 60, 294, 694, 744
BstDEI CTNAG 1 cut(s) 532
BstDSI CCRYGG 1 cut(s) 172
BstF5I GGATG 3 cut(s) 183, 409, 503
BstFNI CGCG 1 cut(s) 60
BstHHI GCGC 2 cut(s) 60, 62
BstKTI GATC 4 cut(s) 30, 34, 712, 818
BstMBI GATC 4 cut(s) 27, 31, 709, 815
BstMCI CGRYCG 2 cut(s) 34, 604
BstNI CCWGG 2 cut(s) 334, 801
BstSCI CCNGG 3 cut(s) 332, 399, 799
BstSNI TACGTA 1 cut(s) 442
BstUI CGCG 1 cut(s) 60
BstV1I GCAGC 2 cut(s) 74, 248
BstV2I GAAGAC 2 cut(s) 660, 756
Bsu15I ATCGAT 1 cut(s) 30
BsuI GTATCC 1 cut(s) 676
BsuRI GGCC 2 cut(s) 202, 502
BsuTUI ATCGAT 1 cut(s) 30
BtgI CCRYGG 1 cut(s) 172
BtsCI GGATG 3 cut(s) 183, 409, 503
BtsIMutI CAGTG 2 cut(s) 314, 577
BveI ACCTGC 2 cut(s) 283, 765
Cac8I GCNNGC 4 cut(s) 60, 294, 694, 744
CaiI CAGNNNCTG 1 cut(s) 372
CfoI GCGC 2 cut(s) 60, 62
Cfr13I GGNCC 5 cut(s) 5, 190, 200, 397, 797
ClaI ATCGAT 1 cut(s) 30
CseI GACGC 1 cut(s) 750
Csp6I GTAC 5 cut(s) 68, 439, 443, 497, 589
CviAII CATG 3 cut(s) 43, 173, 527
CviJI RGCY 7 cut(s) 171, 202, 239, 328, 502, 692, 742
CviKI_1 RGCY 7 cut(s) 171, 202, 239, 328, 502, 692, 742
CviQI GTAC 5 cut(s) 68, 439, 443, 497, 589
DdeI CTNAG 1 cut(s) 532
DpnI GATC 4 cut(s) 29, 33, 711, 817
DpnII GATC 4 cut(s) 27, 31, 709, 815
EaeI YGGCCR 1 cut(s) 500
Eco105I TACGTA 1 cut(s) 442
Eco130I CCWWGG 2 cut(s) 172, 474
Eco47I GGWCC 4 cut(s) 5, 190, 397, 797
EcoO109I RGGNCCY 2 cut(s) 5, 797
EcoRII CCWGG 2 cut(s) 332, 799
EcoT14I CCWWGG 2 cut(s) 172, 474
ErhI CCWWGG 2 cut(s) 172, 474
FaeI CATG 3 cut(s) 46, 176, 530
FaqI GGGAC 3 cut(s) 18, 176, 611
FatI CATG 3 cut(s) 42, 172, 526
Fnu4HI GCNGC 3 cut(s) 63, 237, 740
FokI GGATG 3 cut(s) 190, 416, 490
Fsp4HI GCNGC 3 cut(s) 63, 237, 740
FspBI CTAG 4 cut(s) 351, 647, 771, 813
GlaI GCGC 2 cut(s) 59, 61
GluI GCNGC 3 cut(s) 63, 237, 740
HaeIII GGCC 2 cut(s) 202, 502
HapII CCGG 2 cut(s) 400, 604
HgaI GACGC 1 cut(s) 750
HhaI GCGC 2 cut(s) 60, 62
Hin1I GRCGYC 1 cut(s) 576
Hin1II CATG 3 cut(s) 46, 176, 530
Hin6I GCGC 2 cut(s) 58, 60
HinP1I GCGC 2 cut(s) 58, 60
HindIII AAGCTT 1 cut(s) 690
HinfI GANTC 2 cut(s) 369, 558
HpaII CCGG 2 cut(s) 400, 604
HphI GGTGA 3 cut(s) 5, 445, 463
Hpy188I TCNGA 3 cut(s) 49, 657, 718
Hpy188III TCNNGA 2 cut(s) 458, 625
HpyAV CCTTC 4 cut(s) 49, 105, 338, 673
HpyCH4III ACNGT 1 cut(s) 318
HpyCH4IV ACGT 3 cut(s) 261, 441, 576
HpyCH4V TGCA 5 cut(s) 142, 296, 375, 706, 746
HpyF3I CTNAG 1 cut(s) 532
HpySE526I ACGT 3 cut(s) 261, 441, 576
Hsp92I GRCGYC 1 cut(s) 576
Hsp92II CATG 3 cut(s) 46, 176, 530
HspAI GCGC 2 cut(s) 58, 60
KflI GGGWCCC 1 cut(s) 5
Kzo9I GATC 4 cut(s) 27, 31, 709, 815
LmnI GCTCC 1 cut(s) 325
Lsp1109I GCAGC 2 cut(s) 74, 248
LweI GCATC 3 cut(s) 45, 208, 607
MaeI CTAG 4 cut(s) 351, 647, 771, 813
MaeII ACGT 3 cut(s) 261, 441, 576
MaeIII GTNAC 3 cut(s) 257, 572, 777
MalI GATC 4 cut(s) 29, 33, 711, 817
MboI GATC 4 cut(s) 27, 31, 709, 815
MboII GAAGA 4 cut(s) 626, 660, 756, 759
MfeI CAATTG 1 cut(s) 17
MluCI AATT 8 cut(s) 17, 102, 134, 461, 660, 789, 805, 823
MmeI TCCRAC 1 cut(s) 116
MnlI CCTC 5 cut(s) 316, 352, 400, 590, 635
MseI TTAA 4 cut(s) 146, 725, 822, 826
MspI CCGG 2 cut(s) 400, 604
MspR9I CCNGG 3 cut(s) 334, 401, 801
MunI CAATTG 1 cut(s) 17
MvaI CCWGG 2 cut(s) 334, 801
MvnI CGCG 1 cut(s) 60
NciI CCSGG 1 cut(s) 401
NcoI CCATGG 1 cut(s) 172
NdeII GATC 4 cut(s) 27, 31, 709, 815
NlaIII CATG 3 cut(s) 46, 176, 530
NlaIV GGNNCC 4 cut(s) 6, 7, 192, 798
NmuCI GTSAC 3 cut(s) 257, 572, 777
PacI TTAATTAA 1 cut(s) 826
PauI GCGCGC 1 cut(s) 58
PfeI GAWTC 2 cut(s) 369, 558
PflMI CCANNNNNTGG 2 cut(s) 178, 513
PfoI TCCNGGA 2 cut(s) 399, 799
PkrI GCNGC 3 cut(s) 64, 238, 741
Ple19I CGATCG 1 cut(s) 34
Ppu21I YACGTR 1 cut(s) 442
PpuMI RGGWCCY 2 cut(s) 5, 797
Psp5II RGGWCCY 2 cut(s) 5, 797
Psp6I CCWGG 2 cut(s) 332, 799
PspGI CCWGG 2 cut(s) 332, 799
PspN4I GGNNCC 4 cut(s) 6, 7, 192, 798
PspPI GGNCC 5 cut(s) 5, 190, 200, 397, 797
PspPPI RGGWCCY 2 cut(s) 5, 797
PstNI CAGNNNCTG 1 cut(s) 372
PteI GCGCGC 1 cut(s) 58
PvuI CGATCG 1 cut(s) 34
RsaI GTAC 5 cut(s) 69, 440, 444, 498, 590
RsaNI GTAC 5 cut(s) 68, 439, 443, 497, 589
SaqAI TTAA 4 cut(s) 146, 725, 822, 826
SatI GCNGC 3 cut(s) 63, 237, 740
Sau3AI GATC 4 cut(s) 27, 31, 709, 815
Sau96I GGNCC 5 cut(s) 5, 190, 200, 397, 797
ScrFI CCNGG 3 cut(s) 334, 401, 801
SfaNI GCATC 3 cut(s) 45, 208, 607
SinI GGWCC 4 cut(s) 5, 190, 397, 797
SnaBI TACGTA 1 cut(s) 442
Sse9I AATT 8 cut(s) 17, 102, 134, 461, 660, 789, 805, 823
SsiI CCGC 2 cut(s) 391, 739
SspMI CTAG 4 cut(s) 351, 647, 771, 813
StyD4I CCNGG 3 cut(s) 332, 399, 799
StyI CCWWGG 2 cut(s) 172, 474
TaaI ACNGT 1 cut(s) 318
TaiI ACGT 3 cut(s) 264, 444, 579
TaqI TCGA 1 cut(s) 30
TasI AATT 8 cut(s) 17, 102, 134, 461, 660, 789, 805, 823
TatI WGTACW 1 cut(s) 588
TauI GCSGC 1 cut(s) 742
TfiI GAWTC 2 cut(s) 369, 558
Tru1I TTAA 4 cut(s) 146, 725, 822, 826
Tru9I TTAA 4 cut(s) 146, 725, 822, 826
TscAI CASTG 2 cut(s) 321, 577
TseFI GTSAC 3 cut(s) 257, 572, 777
TseI GCWGC 2 cut(s) 62, 236
Tsp45I GTSAC 3 cut(s) 257, 572, 777
TspDTI ATGAA 4 cut(s) 31, 374, 455, 627
TspGWI ACGGA 1 cut(s) 702
TspRI CASTG 2 cut(s) 321, 577
Van91I CCANNNNNTGG 2 cut(s) 178, 513
VpaK11BI GGWCC 4 cut(s) 5, 190, 397, 797
XapI RAATTY 4 cut(s) 134, 461, 789, 805
XcmI CCANNNNNNNNNTGG 1 cut(s) 510
XspI CTAG 4 cut(s) 351, 647, 771, 813
ZraI GACGTC 1 cut(s) 577
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.