Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr1

Orthogroup ID

OG0008640

Physical Location & Seq

Genomic Coordinates Reverse (-)

20363329 .. 20367071

Gene Structure 3743 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2856 bp

ATGATGCGGAAGTGCCACAAGAACACATGTCCTGTTGGCATCGCAACTCAAGATCCTGTACTTCGTGAGAAGTTTGCTGGAGAACCTGAACATGTTATTAATTTTTTCTTTATGATAGCCGAGGAACTCAGAGAGATTATGGCGCACCTTGGTTTCCGTACTATAAATGAGATGGTTGGTCGTTCAGATATGCTTGAAGTTGATAGAGAAGTGACTAAAAACAATGAGAAGCTTGACACTATCGATCTATCTTTGTTACTTCGACCTGCAGCAGACCTTCGGCCTGGAGCTGCACAATATTGTGTTCAGAAACAAGACCATGGCTTGGATATGGCTCTTGATCACAAGTTAATCGCTATGTCTAAAGCTGCTTTGGAAAAGGCTCTTCCTGTATATTTTGAGACACCAATTTGCAATGTGAACCGTGCTGTTGGAACAATGCTTAGCCACGAGGTGACAAAGCGTTACAACAGGGCAGGGCTTCCTGCTGACACCATTCATATCAAATTTAGTGGAAGTGCAGGGCAGAGTCTTGGAGCATTTTTGTGTTCTGGAATCATGCTTGAGCTTGAGGGTGACAGTAATGATTATGTTGGTAAAGGTTTGTCTGGTGGAAAGATTGTAGTTTATCCTCCCAAGGGAAGCAAGTTTGACCCAAAAGAAAATATTGTCATCGGTAATGTAGCTTTATATGGTGCCACTAGTGGTGAGGCATACTTCAATGGAATGGCAGCAGAAAGATTTTGTGTTCGCAATTCAGGGGCTAAGGCAGTTGTCGAGGGTGTTGGTGATCATGGCTGTGAATATATGACAGGTGGCACTGTTGTTGTGCTCGGGAAAACTGGCAGGAACTTTGCTGCTGGTATGAGTGGTGGTGTTGCCTACGTTTATGATGTGGATGGACAATTTCGGTCTCGATGTAATCCTGAGCTTATAGATCTTGATACACTTGAAGAAGAAGATATTCTGACTCTTCAAATGATGATACAACAACATCAACGCCACACTAACAGCCTGCTGGCCGTTCAAGTACTGGCTGATTTTGAAAATCTTCTTCCTAAGTTTATCAAGGTTATTCCAAGAGAGTACAAGCGTGTTCTTGCAGATATGAAAGAGGAAACCAAACAGGTTATAGAGCACGAGGAGGAAGATGAGCCAGAGCTTGAAGAGAAAGATGCTTTTGAAGAACTTAAGAAGTTGGCGGCTGCATCTTTAAATGGGAAATCCAATCAGGTAGAAGATGCTGAAGCATTGAAGAGGCCTTCTCAGGTCACTGATGCTGTTAAACATCGCGGTTTTATTTCTTATGAGCGCGAGGGCATTCAATATAGGGATCTCAATGTTCGGATGAATGACTGGAAGGAAGTTATGGAGGAAACTAAACCTGGACCACTTGTGAAGACTCAGTCAGCCCGTTGCATGGATTGCGGGACTCCTTTCTGCCATCAGGAGAACACTGGATGCCCTCTTGGTAACAAAATACCAGAGTTCAATGAGTTGGTGTACCAGAATAGGTGGCGTGAAGCATTAGTGAGACTTCTTGAGACCAATAACTTCCCCGAGTTTACTGGTCGGGTTTGTCCGGCACCATGTGAAGGTTCTTGTGTTCTGGGTATTATCGAGAATCCTGTATCTATCAAAAGCATAGAATGTGCCATCATAGACAAGGCTTTTGAGGAAGGGTGGATGGTGCCACGACCTCCTGTAAAGAGAACTGGGAAAAGGGTTGCCATTGTAGGAAGTGGGCCAGCTGGTCTAGCTGCCGCTGATCAGCTAAACAGAATAGGCCACACAGTGACTGTGTATGAGCGTGCTGATAGAATCGGAGGGCTCATGATGTACGGAGTTCCTAATATGAAGACTGATAAAGTGGAGATAGTTCAACGGCGGGTTAACCTTATGGCTGAGGAAGGTGTCAACTTCGTGGTTAACGCTAATATTGGAAATGATCCCTTGTACTCACTTGATAGGCTTCGAGAGGAGAACAATGCCATTGTTTTAGCTGTAGGAGCAACGAAACCCAGGGATCTTCCTGTACCAGGGAGAGAGCTGTCCGGAGTACATTTTGCAATGGAATTTCTACGTGCAAACACCAAGAGCTTATTAGATAGCAATCTCGAGGACGGTAACTACATTTCTGCCAAGGGGAAGAAAGTTGTGGTAATTGGCGGGGGTGACACTGGCACGGATTGCATTGGAACATCCGTACGCCATGGCTGCACTAGCATCATAAATCTGGAGCTTCTCCCTGAGCCACCACGAACAAGGGCTCCAGGAAACCCTTGGCCACAGTGGCCTCGTGTATTCCGTGTGGATTATGGGCACCAAGAAGTTGCAGCTAAGTTTGGCAAAGATCGATCACGAACTTATGAGGTGCTGACAAAGAGATTTGTGGGAGATGAGAATGGGGCCGTGAAAGGACTTGAGGTGGTACGTGTCAAATGGGAGAAGGATGAAACTGGGCGGTTCCAATTCAAGGAAATCGAGGGATCTGAGGAGATCCTCGAGGCAGACCTCGTCCTACTTGCAATGGGGTTCCTTGGACCAGAGGCGACGGTAGCAGAGAAGTTGGGTTTGGAACGTGACCAACGCTCAAACTACAAGGCGGACTATGGTCGGTTCTCAACCAATGTGGATGGGGTGTTTGCCGCTGGGGATTGCCGGCGCGGTCAATCCCTTGTGGTGTGGGCAATCTCAGAGGGAAGGCAAGCCGCTGCACAGGTTGACAAATACCTCTTGAACGAGGAAGAAGACCGCACCATCAGCAACGGGAGCCATCCAGATCTTAGCAAGAGGCATCAAGACCTCAGCAAGAGAAACCCAACAGGTAGCAGTAAACACACAGTGATGACATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006520 GO:0006536 GO:0006537 GO:0006541 GO:0006807 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009064 GO:0009084 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009719 GO:0009725 GO:0009735 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0015930 GO:0016040 GO:0016053 GO:0016491 GO:0016638 GO:0016639 GO:0019676 GO:0019740 GO:0019752 GO:0032502 GO:0040007 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043648 GO:0043650 GO:0044237 GO:0044238 GO:0044249 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0045181 GO:0046394 GO:0046686 GO:0048589 GO:0050896 GO:0055114 GO:0060359 GO:0071704 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607 GO:1901698

KEGG Pathways

Metabolic & Signaling

ko00250 ko00910 ko01100 ko01110 ko01120 ko01130 ko01230 map00250 map00910 map01100 map01110 map01120 map01130 map01230

Pfam Domains

Protein Families

Fer4_20 GATase_2 GXGXG Glu_syn_central Glu_synthase Pyr_redox_2

Protein Analysis

952

Amino Acids

105.34

Weight (kDa)

5.69

Isoelectric Point (pI)

33.63

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glu_synthase	PF01645	1 - 52	1.4e-15	Conserved region in glutamate synthase
GXGXG	PF01493	136 - 321	1.3e-88	GXGXG motif
Fer4_20	PF14691	450 - 561	7.4e-22	Dihydroprymidine dehydrogenase domain II, 4Fe-4S cluster
Pyr_redox_2	PF07992	575 - 901	5.2e-26	Pyridine nucleotide-disulphide oxidoreductase
NAD_binding_8	PF13450	578 - 613	6.8e-09	NAD(P)-binding Rossmann-like domain
Amino_oxidase	PF01593	584 - 612	3.6e-06	Flavin containing amine oxidoreductase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0008640)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G53460 AT5G53460 AT5G53460
fragaria_vesca	FvH4_7g31770 FvH4_7g31770 FvH4_7g31770
malus_domestica	MD01G1219500.v1.1 MD07G1290500.v1.1
prunus_persica	Prupe.2G311700_v2.0.a1 Prupe.2G311700_v2.0.a1 Prupe.2G311700_v2.0.a1
pyrus_communis	pycom01g22930 pycom07g26320
rosa_chinensis	RchiOBHm_Chr1g0381001
rosa_laevigata	RLG00000026253
rosa_multiflora	Rmu_ssc0000032.1_g000001
rosa_roxburghii	Rroxscaffold_4G00278870
rosa_rugosa	Rorug01G0426400
rosa_samantha	Rh1AG450100 Rh1BG406100 Rh1CG420100 Rh1DG436600
rosa_wichuraiana	Rw1G039220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AbsI	CCTCGAGG	1 cut(s)	2504
Acc36I	ACCTGC	1 cut(s)	274
AccB1I	GGYRCC	4 cut(s)	695, 1585, 1690, 2322
AccB7I	CCANNNNNTGG	1 cut(s)	325
AccII	CGCG	3 cut(s)	1293, 1314, 2667
AccIII	TCCGGA	1 cut(s)	2054
AclWI	GGATC	6 cut(s)	47, 1341, 1943, 2034, 2494, 2497
AcoI	YGGCCR	2 cut(s)	1020, 2285
AcsI	RAATTY	2 cut(s)	506, 2075
AcuI	CTGAAG	1 cut(s)	1266
AdeI	CACNNNGTG	4 cut(s)	454, 1592, 1795, 2845
AfiI	CCNNNNNNNGG	6 cut(s)	325, 638, 1420, 1595, 2039, 2476
AflII	CTTAAG	1 cut(s)	1190
AflIII	ACRYGT	3 cut(s)	26, 91, 2434
AhlI	ACTAGT	1 cut(s)	701
AjnI	CCWGG	5 cut(s)	283, 1384, 2021, 2038, 2272
AjuI	GAANNNNNNNTTGG	2 cut(s)	2558, 2590
AloI	GAACNNNNNNTCC	2 cut(s)	2254, 2286
Alw21I	GWGCWC	2 cut(s)	834, 1140
Alw26I	GTCTC	4 cut(s)	395, 918, 1528, 1538
AlwI	GGATC	6 cut(s)	47, 1341, 1943, 2034, 2494, 2497
AlwNI	CAGNNNCTG	1 cut(s)	1799
Ama87I	CYCGRG	4 cut(s)	833, 1559, 2117, 2504
Aor13HI	TCCGGA	1 cut(s)	2054
AoxI	GGCC	8 cut(s)	281, 1020, 1259, 1745, 1786, 2285, 2294, 2409
ApoI	RAATTY	2 cut(s)	506, 2075
ArsI	GACNNNNNNTTYG	2 cut(s)	1655, 1687
AseI	ATTAAT	1 cut(s)	99
Asp700I	GAANNNNTTC	2 cut(s)	963, 1050
AspLEI	GCGC	3 cut(s)	145, 1314, 2667
AspS9I	GGNCC	4 cut(s)	1388, 1745, 2409, 2543
AsuHPI	GGTGA	5 cut(s)	466, 587, 719, 800, 2186
AvaI	CYCGRG	4 cut(s)	833, 1559, 2117, 2504
AvaII	GGWCC	2 cut(s)	1388, 2543
BaeGI	GKGCMC	1 cut(s)	2325
BalI	TGGCCA	1 cut(s)	2287
BanI	GGYRCC	4 cut(s)	695, 1585, 1690, 2322
BanII	GRGCYC	2 cut(s)	1833, 2272
BauI	CACGAG	3 cut(s)	449, 1139, 2298
BbsI	GAAGAC	3 cut(s)	1406, 1865, 2757
Bbv12I	GWGCWC	2 cut(s)	834, 1140
BbvCI	CCTCAGC	2 cut(s)	1905, 2807
BccI	CCATC	8 cut(s)	166, 893, 1452, 1664, 1681, 2630, 2768, 2784
BceAI	ACGGC	3 cut(s)	1007, 1901, 2396
BciT130I	CCWGG	5 cut(s)	285, 1386, 2023, 2040, 2274
BclI	TGATCA	3 cut(s)	340, 790, 1768
BcoDI	GTCTC	4 cut(s)	395, 918, 1528, 1538
BcuI	ACTAGT	1 cut(s)	701
BfaI	CTAG	3 cut(s)	702, 1757, 2223
BfmI	CTRYAG	2 cut(s)	267, 2004
BfrI	CTTAAG	1 cut(s)	1190
BfuAI	ACCTGC	1 cut(s)	274
BglI	GCCNNNNNGGC	1 cut(s)	2293
BglII	AGATCT	2 cut(s)	937, 2782
BlpI	GCTNAGC	1 cut(s)	443
BmcAI	AGTACT	1 cut(s)	1032
Bme1390I	CCNGG	5 cut(s)	285, 1386, 2023, 2040, 2274
Bme18I	GGWCC	2 cut(s)	1388, 2543
BmeT110I	CYCGRG	4 cut(s)	833, 1559, 2117, 2504
BmgT120I	GGNCC	4 cut(s)	1388, 1745, 2409, 2543
BmiI	GGNNCC	9 cut(s)	697, 1587, 1692, 2271, 2324, 2410, 2468, 2537, 2774
BmrFI	CCNGG	5 cut(s)	285, 1386, 2023, 2040, 2274
BmrI	ACTGGG	2 cut(s)	1725, 2469
BmsI	GCATC	8 cut(s)	48, 1165, 1217, 1231, 1267, 1451, 2235, 2806
BmuI	ACTGGG	2 cut(s)	1725, 2469
BoxI	GACNNNNGTC	1 cut(s)	2613
BpiI	GAAGAC	3 cut(s)	1406, 1865, 2757
BplI	GAGNNNNNCTC	2 cut(s)	1249, 1281
BpmI	CTGGAG	4 cut(s)	99, 306, 2256, 2258
Bpu10I	CCTNAGC	5 cut(s)	765, 927, 1905, 2250, 2807
Bpu1102I	GCTNAGC	1 cut(s)	443
BpuEI	CTTGAG	5 cut(s)	33, 584, 590, 1562, 2444
Bsa29I	ATCGAT	2 cut(s)	243, 2356
BsaAI	YACGTR	2 cut(s)	2084, 2435
BsaBI	GATNNNNATC	1 cut(s)	2112
BsaI	GGTCTC	2 cut(s)	918, 1538
BsaWI	WCCGGW	1 cut(s)	2054
BsaXI	ACNNNNNCTCC	2 cut(s)	2254, 2284
Bsc4I	CCNNNNNNNGG	6 cut(s)	325, 638, 1420, 1595, 2039, 2476
Bse118I	RCCGGY	1 cut(s)	2661
Bse1I	ACTGG	8 cut(s)	847, 1038, 1361, 1462, 1573, 1720, 2185, 2464
Bse3DI	GCAATG	3 cut(s)	421, 2076, 2535
Bse8I	GATNNNNATC	1 cut(s)	2112
BseAI	TCCGGA	1 cut(s)	2054
BseBI	CCWGG	5 cut(s)	285, 1386, 2023, 2040, 2274
BseCI	ATCGAT	2 cut(s)	243, 2356
BseGI	GGATG	8 cut(s)	904, 1353, 1466, 1692, 2201, 2458, 2641, 2776
BseJI	GATNNNNATC	1 cut(s)	2112
BseLI	CCNNNNNNNGG	6 cut(s)	325, 638, 1420, 1595, 2039, 2476
BseMI	GCAATG	3 cut(s)	421, 2076, 2535
BseMII	CTCAG	9 cut(s)	142, 918, 1280, 1418, 1896, 2241, 2484, 2709, 2821
BseNI	ACTGG	8 cut(s)	847, 1038, 1361, 1462, 1573, 1720, 2185, 2464
BseRI	GAGGAG	3 cut(s)	1157, 1994, 2510
BseSI	GKGCMC	1 cut(s)	2325
BseYI	CCCAGC	1 cut(s)	2651
BsgI	GTGCAG	4 cut(s)	276, 540, 2203, 2700
Bsh1236I	CGCG	3 cut(s)	1293, 1314, 2667
BshFI	GGCC	8 cut(s)	283, 1022, 1261, 1747, 1788, 2287, 2296, 2411
BshNI	GGYRCC	4 cut(s)	695, 1585, 1690, 2322
BshVI	ATCGAT	2 cut(s)	243, 2356
BsiHKAI	GWGCWC	2 cut(s)	834, 1140
BsiHKCI	CYCGRG	4 cut(s)	833, 1559, 2117, 2504
BsiSI	CCGG	3 cut(s)	1583, 2055, 2662
BsiWI	CGTACG	1 cut(s)	2206
BslFI	GGGAC	1 cut(s)	1444
BslI	CCNNNNNNNGG	6 cut(s)	325, 638, 1420, 1595, 2039, 2476
BsmAI	GTCTC	4 cut(s)	395, 918, 1528, 1538
BsmFI	GGGAC	1 cut(s)	1444
BsmI	GAATGC	1 cut(s)	1320
BsnI	GGCC	8 cut(s)	283, 1022, 1261, 1747, 1788, 2287, 2296, 2411
Bso31I	GGTCTC	2 cut(s)	918, 1538
BsoBI	CYCGRG	4 cut(s)	833, 1559, 2117, 2504
Bsp1286I	GDGCHC	5 cut(s)	834, 1140, 1833, 2272, 2325
Bsp13I	TCCGGA	1 cut(s)	2054
Bsp1720I	GCTNAGC	1 cut(s)	443
Bsp19I	CCATGG	2 cut(s)	319, 2212
BspANI	GGCC	8 cut(s)	283, 1022, 1261, 1747, 1788, 2287, 2296, 2411
BspCNI	CTCAG	9 cut(s)	141, 919, 1279, 1417, 1897, 2242, 2485, 2708, 2820
BspDI	ATCGAT	2 cut(s)	243, 2356
BspEI	TCCGGA	1 cut(s)	2054
BspFNI	CGCG	3 cut(s)	1293, 1314, 2667
BspHI	TCATGA	1 cut(s)	1833
BspLI	GGNNCC	9 cut(s)	697, 1587, 1692, 2271, 2324, 2410, 2468, 2537, 2774
BspMAI	CTGCAG	1 cut(s)	271
BspMI	ACCTGC	1 cut(s)	274
BspPI	GGATC	6 cut(s)	47, 1341, 1943, 2034, 2494, 2497
BspQI	GCTCTTC	1 cut(s)	390
BspT107I	GGYRCC	4 cut(s)	695, 1585, 1690, 2322
BspTI	CTTAAG	1 cut(s)	1190
BspTNI	GGTCTC	2 cut(s)	918, 1538
BsrDI	GCAATG	3 cut(s)	421, 2076, 2535
BsrFI	RCCGGY	1 cut(s)	2661
BsrI	ACTGG	8 cut(s)	847, 1038, 1361, 1462, 1573, 1720, 2185, 2464
BssAI	RCCGGY	1 cut(s)	2661
BssSI	CACGAG	3 cut(s)	449, 1139, 2298
BssT1I	CCWWGG	7 cut(s)	148, 319, 636, 2142, 2212, 2282, 2539
Bst2BI	CACGAG	3 cut(s)	449, 1139, 2298
Bst2UI	CCWGG	5 cut(s)	285, 1386, 2023, 2040, 2274
Bst4CI	ACNGT	9 cut(s)	425, 581, 823, 1795, 1801, 2126, 2292, 2557, 2845
Bst6I	CTCTTC	4 cut(s)	390, 978, 1161, 1250
BstAFI	CTTAAG	1 cut(s)	1190
BstAPI	GCANNNNNTGC	2 cut(s)	1425, 2190
BstBAI	YACGTR	2 cut(s)	2084, 2435
BstC8I	GCNNGC	6 cut(s)	1016, 1020, 1749, 1812, 2663, 2709
BstDSI	CCRYGG	2 cut(s)	319, 2212
BstENI	CCTNNNNNAGG	1 cut(s)	2037
BstF5I	GGATG	8 cut(s)	904, 1353, 1466, 1692, 2201, 2458, 2641, 2776
BstFNI	CGCG	3 cut(s)	1293, 1314, 2667
BstHHI	GCGC	3 cut(s)	145, 1314, 2667
BstMAI	GTCTC	4 cut(s)	395, 918, 1528, 1538
BstNI	CCWGG	5 cut(s)	285, 1386, 2023, 2040, 2274
BstNSI	RCATGY	2 cut(s)	30, 95
BstPAI	GACNNNNGTC	1 cut(s)	2613
BstSCI	CCNGG	5 cut(s)	283, 1384, 2021, 2038, 2272
BstSFI	CTRYAG	2 cut(s)	267, 2004
BstSLI	GKGCMC	1 cut(s)	2325
BstUI	CGCG	3 cut(s)	1293, 1314, 2667
BstV2I	GAAGAC	3 cut(s)	1406, 1865, 2757
BstX2I	RGATCY	7 cut(s)	52, 937, 1333, 2026, 2489, 2499, 2782
BstYI	RGATCY	7 cut(s)	52, 937, 1333, 2026, 2489, 2499, 2782
Bsu15I	ATCGAT	2 cut(s)	243, 2356
BsuRI	GGCC	8 cut(s)	283, 1022, 1261, 1747, 1788, 2287, 2296, 2411
BsuTUI	ATCGAT	2 cut(s)	243, 2356
BtgI	CCRYGG	2 cut(s)	319, 2212
BtgZI	GCGATG	2 cut(s)	25, 1274
BtsCI	GGATG	8 cut(s)	904, 1353, 1466, 1692, 2201, 2458, 2641, 2776
BtsIMutI	CAGTG	7 cut(s)	819, 1272, 1455, 1800, 2178, 2297, 2850
BveI	ACCTGC	1 cut(s)	274
Cac8I	GCNNGC	6 cut(s)	1016, 1020, 1749, 1812, 2663, 2709
CaiI	CAGNNNCTG	1 cut(s)	1799
CciI	TCATGA	1 cut(s)	1833
CfoI	GCGC	3 cut(s)	145, 1314, 2667
Cfr10I	RCCGGY	1 cut(s)	2661
Cfr13I	GGNCC	4 cut(s)	1388, 1745, 2409, 2543
ClaI	ATCGAT	2 cut(s)	243, 2356
CspCI	CAANNNNNGTGG	4 cut(s)	493, 528, 2613, 2648
CviAII	CATG	9 cut(s)	27, 92, 320, 559, 794, 1420, 1590, 1834, 2213
DraI	TTTAAA	1 cut(s)	1215
DraIII	CACNNNGTG	4 cut(s)	454, 1592, 1795, 2845
EaeI	YGGCCR	2 cut(s)	1020, 2285
Eam1104I	CTCTTC	4 cut(s)	390, 978, 1161, 1250
EarI	CTCTTC	4 cut(s)	390, 978, 1161, 1250
EciI	GGCGGA	1 cut(s)	2621
Eco130I	CCWWGG	7 cut(s)	148, 319, 636, 2142, 2212, 2282, 2539
Eco147I	AGGCCT	1 cut(s)	1261
Eco24I	GRGCYC	2 cut(s)	1833, 2272
Eco31I	GGTCTC	2 cut(s)	918, 1538
Eco47I	GGWCC	2 cut(s)	1388, 2543
Eco57I	CTGAAG	1 cut(s)	1266
Eco88I	CYCGRG	4 cut(s)	833, 1559, 2117, 2504
EcoNI	CCTNNNNNAGG	1 cut(s)	2037
EcoRII	CCWGG	5 cut(s)	283, 1384, 2021, 2038, 2272
EcoT14I	CCWWGG	7 cut(s)	148, 319, 636, 2142, 2212, 2282, 2539
EcoT38I	GRGCYC	2 cut(s)	1833, 2272
ErhI	CCWWGG	7 cut(s)	148, 319, 636, 2142, 2212, 2282, 2539
FaeI	CATG	9 cut(s)	30, 95, 323, 562, 797, 1423, 1593, 1837, 2216
FaqI	GGGAC	1 cut(s)	1444
FatI	CATG	9 cut(s)	26, 91, 319, 558, 793, 1419, 1589, 1833, 2212
FauI	CCCGC	3 cut(s)	1421, 1881, 2162
FbaI	TGATCA	3 cut(s)	340, 790, 1768
FokI	GGATG	8 cut(s)	911, 1360, 1473, 1699, 2188, 2465, 2648, 2763
FriOI	GRGCYC	2 cut(s)	1833, 2272
FspBI	CTAG	3 cut(s)	702, 1757, 2223
GlaI	GCGC	3 cut(s)	144, 1313, 2666
GsaI	CCCAGC	1 cut(s)	2655
GsuI	CTGGAG	4 cut(s)	99, 306, 2256, 2258
HaeIII	GGCC	8 cut(s)	283, 1022, 1261, 1747, 1788, 2287, 2296, 2411
HapII	CCGG	3 cut(s)	1583, 2055, 2662
HhaI	GCGC	3 cut(s)	145, 1314, 2667
Hin1II	CATG	9 cut(s)	30, 95, 323, 562, 797, 1423, 1593, 1837, 2216
Hin6I	GCGC	3 cut(s)	143, 1312, 2665
HinP1I	GCGC	3 cut(s)	143, 1312, 2665
HincII	GTYRAC	4 cut(s)	1894, 1918, 1930, 2725
HindII	GTYRAC	4 cut(s)	1894, 1918, 1930, 2725
HindIII	AAGCTT	1 cut(s)	230
HinfI	GANTC	7 cut(s)	529, 555, 970, 1402, 1432, 1624, 1821
HpaI	GTTAAC	2 cut(s)	1894, 1930
HpaII	CCGG	3 cut(s)	1583, 2055, 2662
HphI	GGTGA	5 cut(s)	466, 587, 719, 800, 2186
Hpy166II	GTNNAC	8 cut(s)	421, 1504, 1566, 1894, 1918, 1930, 2725, 2837
Hpy188I	TCNGA	8 cut(s)	131, 187, 309, 969, 1347, 1826, 2494, 2698
Hpy8I	GTNNAC	8 cut(s)	421, 1504, 1566, 1894, 1918, 1930, 2725, 2837
Hpy99I	CGWCG	1 cut(s)	2557
HpyAV	CCTTC	8 cut(s)	287, 1272, 1354, 1589, 1673, 1904, 2443, 2697
HpyCH4III	ACNGT	9 cut(s)	425, 581, 823, 1795, 1801, 2126, 2292, 2557, 2845
HpyCH4IV	ACGT	4 cut(s)	885, 2083, 2434, 2581
HpySE526I	ACGT	4 cut(s)	885, 2083, 2434, 2581
Hsp92II	CATG	9 cut(s)	30, 95, 323, 562, 797, 1423, 1593, 1837, 2216
HspAI	GCGC	3 cut(s)	143, 1312, 2665
Kpn2I	TCCGGA	1 cut(s)	2054
KroI	GCCGGC	1 cut(s)	2661
KroNI	GCCGGC	1 cut(s)	2663
Ksp22I	TGATCA	3 cut(s)	340, 790, 1768
KspAI	GTTAAC	2 cut(s)	1894, 1930
LguI	GCTCTTC	1 cut(s)	390
LmnI	GCTCC	6 cut(s)	287, 536, 2009, 2239, 2275, 2772
LweI	GCATC	8 cut(s)	48, 1165, 1217, 1231, 1267, 1451, 2235, 2806
MaeI	CTAG	3 cut(s)	702, 1757, 2223
MaeII	ACGT	4 cut(s)	885, 2083, 2434, 2581
MflI	RGATCY	7 cut(s)	52, 937, 1333, 2026, 2489, 2499, 2782
MhlI	GDGCHC	5 cut(s)	834, 1140, 1833, 2272, 2325
MlsI	TGGCCA	1 cut(s)	2287
MluCI	AATT	8 cut(s)	100, 408, 506, 754, 905, 2075, 2163, 2471
MluNI	TGGCCA	1 cut(s)	2287
MlyI	GAGTC	4 cut(s)	538, 964, 1396, 1426
MmeI	TCCRAC	1 cut(s)	412
Mox20I	TGGCCA	1 cut(s)	2287
MroI	TCCGGA	1 cut(s)	2054
MroNI	GCCGGC	1 cut(s)	2661
MroXI	GAANNNNTTC	2 cut(s)	963, 1050
MscI	TGGCCA	1 cut(s)	2287
MseI	TTAA	7 cut(s)	99, 350, 1191, 1214, 1284, 1893, 1929
MslI	CAYNNNNRTG	3 cut(s)	544, 798, 2846
Msp20I	TGGCCA	1 cut(s)	2287
MspA1I	CMGCKG	4 cut(s)	1751, 1766, 2651, 2714
MspCI	CTTAAG	1 cut(s)	1190
MspI	CCGG	3 cut(s)	1583, 2055, 2662
MspR9I	CCNGG	5 cut(s)	285, 1386, 2023, 2040, 2274
Mva1269I	GAATGC	1 cut(s)	1320
MvaI	CCWGG	5 cut(s)	285, 1386, 2023, 2040, 2274
MvnI	CGCG	3 cut(s)	1293, 1314, 2667
NaeI	GCCGGC	1 cut(s)	2663
NcoI	CCATGG	2 cut(s)	319, 2212
NgoMIV	GCCGGC	1 cut(s)	2661
NlaIII	CATG	9 cut(s)	30, 95, 323, 562, 797, 1423, 1593, 1837, 2216
NlaIV	GGNNCC	9 cut(s)	697, 1587, 1692, 2271, 2324, 2410, 2468, 2537, 2774
NmeAIII	GCCGAG	1 cut(s)	145
NmuCI	GTSAC	7 cut(s)	211, 454, 575, 1270, 1795, 2174, 2582
NspI	RCATGY	2 cut(s)	30, 95
PaeR7I	CTCGAG	2 cut(s)	2117, 2504
PagI	TCATGA	1 cut(s)	1833
PasI	CCCWGGG	1 cut(s)	2022
PceI	AGGCCT	1 cut(s)	1261
PciI	ACATGT	2 cut(s)	26, 91
PciSI	GCTCTTC	1 cut(s)	390
PcsI	WCGNNNNNNNCGW	1 cut(s)	2305
PctI	GAATGC	1 cut(s)	1320
PdiI	GCCGGC	1 cut(s)	2663
PdmI	GAANNNNTTC	2 cut(s)	963, 1050
PfeI	GAWTC	3 cut(s)	555, 1624, 1821
Pfl23II	CGTACG	1 cut(s)	2206
PflFI	GACNNNGTC	2 cut(s)	1405, 2516
PflMI	CCANNNNNTGG	1 cut(s)	325
PfoI	TCCNGGA	1 cut(s)	2272
PleI	GAGTC	4 cut(s)	537, 964, 1396, 1426
PpsI	GAGTC	4 cut(s)	537, 964, 1396, 1426
Ppu21I	YACGTR	2 cut(s)	2084, 2435
PscI	ACATGT	2 cut(s)	26, 91
PshAI	GACNNNNGTC	1 cut(s)	2613
PshBI	ATTAAT	1 cut(s)	99
Psp6I	CCWGG	5 cut(s)	283, 1384, 2021, 2038, 2272
PspFI	CCCAGC	1 cut(s)	2651
PspGI	CCWGG	5 cut(s)	283, 1384, 2021, 2038, 2272
PspLI	CGTACG	1 cut(s)	2206
PspN4I	GGNNCC	9 cut(s)	697, 1587, 1692, 2271, 2324, 2410, 2468, 2537, 2774
PspPI	GGNCC	4 cut(s)	1388, 1745, 2409, 2543
PspXI	VCTCGAGB	1 cut(s)	2504
PstI	CTGCAG	1 cut(s)	271
PstNI	CAGNNNCTG	1 cut(s)	1799
PsuI	RGATCY	7 cut(s)	52, 937, 1333, 2026, 2489, 2499, 2782
PsyI	GACNNNGTC	2 cut(s)	1405, 2516
PvuII	CAGCTG	1 cut(s)	1751
RseI	CAYNNNNRTG	3 cut(s)	544, 798, 2846
SapI	GCTCTTC	1 cut(s)	390
SaqAI	TTAA	7 cut(s)	99, 350, 1191, 1214, 1284, 1893, 1929
Sau96I	GGNCC	4 cut(s)	1388, 1745, 2409, 2543
ScaI	AGTACT	1 cut(s)	1032
SchI	GAGTC	4 cut(s)	538, 964, 1396, 1426
ScrFI	CCNGG	5 cut(s)	285, 1386, 2023, 2040, 2274
SduI	GDGCHC	5 cut(s)	834, 1140, 1833, 2272, 2325
SfaNI	GCATC	8 cut(s)	48, 1165, 1217, 1231, 1267, 1451, 2235, 2806
SfcI	CTRYAG	2 cut(s)	267, 2004
SfiI	GGCCNNNNNGGCC	1 cut(s)	2293
Sfr274I	CTCGAG	2 cut(s)	2117, 2504
SinI	GGWCC	2 cut(s)	1388, 2543
SlaI	CTCGAG	2 cut(s)	2117, 2504
SmiMI	CAYNNNNRTG	3 cut(s)	544, 798, 2846
SmlI	CTYRAG	8 cut(s)	48, 563, 569, 1190, 1541, 2117, 2423, 2504
SmoI	CTYRAG	8 cut(s)	48, 563, 569, 1190, 1541, 2117, 2423, 2504
SpeI	ACTAGT	1 cut(s)	701
Sse9I	AATT	8 cut(s)	100, 408, 506, 754, 905, 2075, 2163, 2471
SseBI	AGGCCT	1 cut(s)	1261
SspI	AATATT	3 cut(s)	299, 667, 1939
SspMI	CTAG	3 cut(s)	702, 1757, 2223
StuI	AGGCCT	1 cut(s)	1261
StyD4I	CCNGG	5 cut(s)	283, 1384, 2021, 2038, 2272
StyI	CCWWGG	7 cut(s)	148, 319, 636, 2142, 2212, 2282, 2539
TaaI	ACNGT	9 cut(s)	425, 581, 823, 1795, 1801, 2126, 2292, 2557, 2845
TaiI	ACGT	4 cut(s)	888, 2086, 2437, 2584
TaqII	GACCGA	1 cut(s)	900
TasI	AATT	8 cut(s)	100, 408, 506, 754, 905, 2075, 2163, 2471
TatI	WGTACW	5 cut(s)	58, 1030, 1086, 1956, 2059
TauI	GCSGC	4 cut(s)	1205, 1766, 2651, 2714
TfiI	GAWTC	3 cut(s)	555, 1624, 1821
Tru1I	TTAA	7 cut(s)	99, 350, 1191, 1214, 1284, 1893, 1929
Tru9I	TTAA	7 cut(s)	99, 350, 1191, 1214, 1284, 1893, 1929
TscAI	CASTG	7 cut(s)	826, 1279, 1462, 1800, 2185, 2297, 2850
TseFI	GTSAC	7 cut(s)	211, 454, 575, 1270, 1795, 2174, 2582
Tsp45I	GTSAC	7 cut(s)	211, 454, 575, 1270, 1795, 2174, 2582
TspDTI	ATGAA	5 cut(s)	488, 1124, 1364, 1871, 2469
TspGWI	ACGGA	5 cut(s)	146, 1857, 2194, 2201, 2297
TspRI	CASTG	7 cut(s)	826, 1279, 1462, 1800, 2185, 2297, 2850
Tth111I	GACNNNGTC	2 cut(s)	1405, 2516
Van91I	CCANNNNNTGG	1 cut(s)	325
Vha464I	CTTAAG	1 cut(s)	1190
VpaK11BI	GGWCC	2 cut(s)	1388, 2543
VspI	ATTAAT	1 cut(s)	99
XagI	CCTNNNNNAGG	1 cut(s)	2037
XapI	RAATTY	2 cut(s)	506, 2075
XceI	RCATGY	2 cut(s)	30, 95
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2280
XhoI	CTCGAG	2 cut(s)	2117, 2504
XmnI	GAANNNNTTC	2 cut(s)	963, 1050
XspI	CTAG	3 cut(s)	702, 1757, 2223
ZrmI	AGTACT	1 cut(s)	1032

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.