pycom01g22970

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0007346
Physical Location & Seq
Forward (+)
20403908 .. 20406773
2866 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g22970.1

Sequence Viewer

Length: 663 bp
ATGACGAGGAGTTCAAGGCACAAATCAAGCAAGCATAGCTCGAGGGATACCAGGGAGTATTCGGATTCGGAGAAAGATTCGAGCTTAAAGGATCGAAGGGGCAAGGAAGAGAGTGGTGTAGTTAGGGTTTCAAAGGATTCAAGTTCGAGTGAGAAGCGGAAGCTTGATTTAAAGGATGGGAAGGACTCGGGTGGCGGCTCCGGGAATGGGGACTATTCGGTGGATTTAGTATCGACTAAGCGGCGCAAGGAGAGGCTTGATGATGGAGGGAGTGATAGGTGGAATGGAGGCGATGATGATCACCGAAGAAGCAGTGAGGGGTCGAAGAAGTCGTTGAAGGCATCAGGGGAGTCTAAGAGTAAGAAAAGAGATGAGAGTGTGGAATTGTATGCAGAGGGTGGCGAAGTGAAGAAGAATTCTAGTAGTAGTGGAAAGGGTGAAGGGAGGCATAGGGATAAGGATAAGGAGTCAATTCGAAAGGAGGGCAAGGAAGGTGGTGGAGCAGAGAGGGAGAAGGAAAGGGACAGGGAAAAGGAGAGGGACAGGGAGAGAGAAAGGGAAAAGAAAGGTAAAGATGGGAGAACCGAGCGATTGGTCAGTAGCGACGAACAACGTGTCACTGCAAAGCAAGTCAATGAAAAAACTGTTAAATGCATGGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

221

Amino Acids

24.67

Weight (kDa)

9.53

Isoelectric Point (pI)

52.45

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 157, 195, 241
AclWI GGATC 1 cut(s) 99
AcsI RAATTY 1 cut(s) 415
AfiI CCNNNNNNNGG 1 cut(s) 207
AflIII ACRYGT 1 cut(s) 613
AgsI TTSAA 4 cut(s) 15, 132, 141, 337
AjnI CCWGG 1 cut(s) 50
AluBI AGCT 3 cut(s) 39, 84, 163
AluI AGCT 3 cut(s) 39, 84, 163
AlwI GGATC 1 cut(s) 99
Ama87I CYCGRG 2 cut(s) 40, 187
ApoI RAATTY 1 cut(s) 415
ArsI GACNNNNNNTTYG 2 cut(s) 600, 632
AspLEI GCGC 1 cut(s) 246
AsuC2I CCSGG 1 cut(s) 202
AsuHPI GGTGA 2 cut(s) 293, 449
AsuII TTCGAA 1 cut(s) 475
AvaI CYCGRG 2 cut(s) 40, 187
BccI CCATC 3 cut(s) 170, 257, 569
BciT130I CCWGG 1 cut(s) 52
BciVI GTATCC 1 cut(s) 40
BclI TGATCA 1 cut(s) 298
BcnI CCSGG 1 cut(s) 202
BfaI CTAG 1 cut(s) 420
BfuI GTATCC 1 cut(s) 40
BisI GCNGC 2 cut(s) 196, 242
BlsI GCNGC 2 cut(s) 197, 243
Bme1390I CCNGG 2 cut(s) 52, 202
BmeT110I CYCGRG 2 cut(s) 40, 187
BmiI GGNNCC 1 cut(s) 199
BmrFI CCNGG 2 cut(s) 52, 202
BmsI GCATC 1 cut(s) 350
Bpu14I TTCGAA 1 cut(s) 475
BpuMI CCSGG 1 cut(s) 202
BsaBI GATNNNNATC 1 cut(s) 297
BsaJI CCNNGG 1 cut(s) 51
Bsc4I CCNNNNNNNGG 1 cut(s) 207
Bse8I GATNNNNATC 1 cut(s) 297
BseBI CCWGG 1 cut(s) 52
BseDI CCNNGG 1 cut(s) 51
BseGI GGATG 1 cut(s) 181
BseJI GATNNNNATC 1 cut(s) 297
BseLI CCNNNNNNNGG 1 cut(s) 207
BseRI GAGGAG 1 cut(s) 22
BsiHKCI CYCGRG 2 cut(s) 40, 187
BsiSI CCGG 1 cut(s) 201
BslFI GGGAC 3 cut(s) 224, 536, 554
BslI CCNNNNNNNGG 1 cut(s) 207
BsmFI GGGAC 3 cut(s) 224, 536, 554
BsoBI CYCGRG 2 cut(s) 40, 187
Bsp119I TTCGAA 1 cut(s) 475
Bsp143I GATC 2 cut(s) 91, 298
BspACI CCGC 3 cut(s) 157, 195, 241
BspLI GGNNCC 1 cut(s) 199
BspPI GGATC 1 cut(s) 99
BspT104I TTCGAA 1 cut(s) 475
BssECI CCNNGG 1 cut(s) 51
BssMI GATC 2 cut(s) 91, 298
Bst2UI CCWGG 1 cut(s) 52
Bst4CI ACNGT 1 cut(s) 646
Bst6I CTCTTC 1 cut(s) 102
BstBI TTCGAA 1 cut(s) 475
BstC8I GCNNGC 1 cut(s) 32
BstDEI CTNAG 2 cut(s) 237, 354
BstF5I GGATG 1 cut(s) 181
BstHHI GCGC 1 cut(s) 246
BstKTI GATC 2 cut(s) 94, 301
BstMBI GATC 2 cut(s) 91, 298
BstMWI GCNNNNNNNGC 1 cut(s) 36
BstNI CCWGG 1 cut(s) 52
BstSCI CCNGG 2 cut(s) 50, 200
BsuI GTATCC 1 cut(s) 40
BtgZI GCGATG 1 cut(s) 306
BtsCI GGATG 1 cut(s) 181
BtsI GCAGTG 2 cut(s) 319, 618
BtsIMutI CAGTG 2 cut(s) 319, 618
Cac8I GCNNGC 1 cut(s) 32
CfoI GCGC 1 cut(s) 246
CspCI CAANNNNNGTGG 2 cut(s) 475, 510
CviAII CATG 1 cut(s) 655
CviJI RGCY 5 cut(s) 39, 84, 163, 198, 256
CviKI_1 RGCY 5 cut(s) 39, 84, 163, 198, 256
DdeI CTNAG 2 cut(s) 237, 354
DpnI GATC 2 cut(s) 93, 300
DpnII GATC 2 cut(s) 91, 298
DraI TTTAAA 1 cut(s) 171
Eam1104I CTCTTC 1 cut(s) 102
EarI CTCTTC 1 cut(s) 102
Eco88I CYCGRG 2 cut(s) 40, 187
EcoRI GAATTC 1 cut(s) 415
EcoRII CCWGG 1 cut(s) 50
EcoT22I ATGCAT 1 cut(s) 656
FaeI CATG 1 cut(s) 658
FaiI YATR 4 cut(s) 36, 390, 450, 656
FaqI GGGAC 3 cut(s) 224, 536, 554
FatI CATG 1 cut(s) 654
FbaI TGATCA 1 cut(s) 298
Fnu4HI GCNGC 2 cut(s) 196, 242
FokI GGATG 1 cut(s) 188
Fsp4HI GCNGC 2 cut(s) 196, 242
FspBI CTAG 1 cut(s) 420
GlaI GCGC 1 cut(s) 245
GluI GCNGC 2 cut(s) 196, 242
HapII CCGG 1 cut(s) 201
HhaI GCGC 1 cut(s) 246
Hin1II CATG 1 cut(s) 658
Hin6I GCGC 1 cut(s) 244
HinP1I GCGC 1 cut(s) 244
HindIII AAGCTT 1 cut(s) 161
HinfI GANTC 6 cut(s) 65, 77, 137, 185, 350, 467
HpaII CCGG 1 cut(s) 201
HphI GGTGA 2 cut(s) 293, 449
Hpy188I TCNGA 2 cut(s) 64, 70
Hpy99I CGWCG 1 cut(s) 608
HpyAV CCTTC 6 cut(s) 90, 175, 331, 434, 485, 508
HpyCH4III ACNGT 1 cut(s) 646
HpyCH4IV ACGT 1 cut(s) 613
HpyCH4V TGCA 3 cut(s) 392, 623, 654
HpyF10VI GCNNNNNNNGC 1 cut(s) 36
HpyF3I CTNAG 2 cut(s) 237, 354
HpySE526I ACGT 1 cut(s) 613
Hsp92II CATG 1 cut(s) 658
HspAI GCGC 1 cut(s) 244
Ksp22I TGATCA 1 cut(s) 298
Kzo9I GATC 2 cut(s) 91, 298
LmnI GCTCC 2 cut(s) 203, 500
LpnPI CCDG 6 cut(s) 37, 64, 214, 330, 511, 529
LweI GCATC 1 cut(s) 350
MaeI CTAG 1 cut(s) 420
MaeII ACGT 1 cut(s) 613
MaeIII GTNAC 1 cut(s) 616
MalI GATC 2 cut(s) 93, 300
MboI GATC 2 cut(s) 91, 298
MboII GAAGA 5 cut(s) 119, 318, 337, 421, 424
MluCI AATT 3 cut(s) 383, 415, 471
MlyI GAGTC 3 cut(s) 179, 359, 476
Mph1103I ATGCAT 1 cut(s) 656
MseI TTAA 3 cut(s) 86, 170, 648
MspI CCGG 1 cut(s) 201
MspR9I CCNGG 2 cut(s) 52, 202
MvaI CCWGG 1 cut(s) 52
MwoI GCNNNNNNNGC 1 cut(s) 36
NciI CCSGG 1 cut(s) 202
NdeII GATC 2 cut(s) 91, 298
NlaIII CATG 1 cut(s) 658
NlaIV GGNNCC 1 cut(s) 199
NmuCI GTSAC 1 cut(s) 616
NsiI ATGCAT 1 cut(s) 656
NspV TTCGAA 1 cut(s) 475
PaeR7I CTCGAG 1 cut(s) 40
PcsI WCGNNNNNNNCGW 1 cut(s) 329
PfeI GAWTC 3 cut(s) 65, 77, 137
PfoI TCCNGGA 1 cut(s) 200
PkrI GCNGC 2 cut(s) 197, 243
PleI GAGTC 3 cut(s) 179, 358, 475
PpsI GAGTC 3 cut(s) 179, 358, 475
Psp6I CCWGG 1 cut(s) 50
PspGI CCWGG 1 cut(s) 50
PspN4I GGNNCC 1 cut(s) 199
PspXI VCTCGAGB 1 cut(s) 40
SaqAI TTAA 3 cut(s) 86, 170, 648
SatI GCNGC 2 cut(s) 196, 242
Sau3AI GATC 2 cut(s) 91, 298
SchI GAGTC 3 cut(s) 179, 359, 476
ScrFI CCNGG 2 cut(s) 52, 202
SetI ASST 7 cut(s) 41, 86, 165, 281, 496, 571, 616
SfaNI GCATC 1 cut(s) 350
Sfr274I CTCGAG 1 cut(s) 40
SfuI TTCGAA 1 cut(s) 475
SlaI CTCGAG 1 cut(s) 40
SmlI CTYRAG 1 cut(s) 40
SmoI CTYRAG 1 cut(s) 40
Sse9I AATT 3 cut(s) 383, 415, 471
SsiI CCGC 3 cut(s) 157, 195, 241
SspMI CTAG 1 cut(s) 420
StyD4I CCNGG 2 cut(s) 50, 200
TaaI ACNGT 1 cut(s) 646
TaiI ACGT 1 cut(s) 616
TaqI TCGA 7 cut(s) 41, 80, 94, 146, 233, 323, 475
TasI AATT 3 cut(s) 383, 415, 471
TauI GCSGC 2 cut(s) 198, 244
TfiI GAWTC 3 cut(s) 65, 77, 137
Tru1I TTAA 3 cut(s) 86, 170, 648
Tru9I TTAA 3 cut(s) 86, 170, 648
TscAI CASTG 2 cut(s) 319, 625
TseFI GTSAC 1 cut(s) 616
Tsp45I GTSAC 1 cut(s) 616
TspDTI ATGAA 1 cut(s) 651
TspRI CASTG 2 cut(s) 319, 625
XapI RAATTY 1 cut(s) 415
XhoI CTCGAG 1 cut(s) 40
XspI CTAG 1 cut(s) 420
Zsp2I ATGCAT 1 cut(s) 656
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.