pycom02g00530

U1-like zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0008822
Physical Location & Seq
Forward (+)
312570 .. 314980
2411 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1239 bp
ATGCTCGAGCGGGAAATCGCCATGCGAAGACCCGCTGGCGAAGGGCTCAGTTTTGATGAGGACCGGTTCGGCGCGATGCGGTTCGACCACAGAGTCCGCCACCCCTTCGATGATGACCGGTTCGGATTCTTGGCTGCTCATGCGCCCGCCACATTCGATAGTTTCACGTGGCGGCAGCAACCGGAACCTCTGACCAATGGCTTCAAGTCGCATCCGGCTCCTCCTCCTGCTTTAGACCTCAATAACAAGGACAAGTTGATCATACTGGCGAAACCTGACCCAAATCTCTCCGGAGTCAAGCGGAAAACACCACCAACAGTGAGTGCAAGTGAGCTACCCCCATTTGGGTTGAAAAAGAAACCCAAGGAGGAATGGAGCTGCGCCCTGTGCCATGTTAGTGCAACAAGTGAGAAAGGCTTGATTCATCACATTAACGGTAAGAAGCACCAAGCCAAGGAAGCAAGACTGAGAGCTCAGAAACCGAGCTCCAGCAATGCACAATCATCAAAGAAAGCTGCAAACTTTTCTGAGCCTAAGAAGATCAACGGTAACAGAAGCACAGAATTTAGGGCCAAACAAGTTAGAAAATCGCTTGACCATAATGAAACCAGTGACGTTTCCAACCAGAAAATGCAGGAGCAAGGCACTTCAAAAAAGGAAAAAGAGGAGCCGCCAATGCTGAAAGACCAGTGTGGAGAGGTTCTCAAGAACAAGGACGAGGTTAAAATGGTGGGAGGAGTGGAGAGAAAAGAAGATGTAAAGAAACACAAGACATTTAAGTTTTGGTGTGAAAGTTGTAAAATTGGTGCCTATAATCCAAAGGTGTGGTCGTCTCATATGAGTGGGAAGAAGCACATTGCTAGGAGTCAGGAACTTACACAATATAATGTTCCGATTGCAAGTTTCATGGCATCATCGGAGGCTAGCAAGGACGCAGAAGATACTGATACGGCCAAAGAAGCTCAGGAGAAGATTCCAACAACCACTGCCATTGCATCATCAGAGGCTAGTATCGAAGCAGAAGATTCTGATGCGGCCAAAGAAGCGCACCAGAAGATTCCAACACCCACTGCCATACCATCAGTAGAGGCTAGTAAGGATGCAGAAGAGGTTGACGCGGCCAAAGAACAGGAGAAGTTCAATGGCGTCATGATCGAGCTAATGAGAATTCATAACACTGCTGTGGCAAAAGAAGCAAAGAACAGTGCGGAAATTGATGTTGCAGACACAGATAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-met

Protein Analysis

413

Amino Acids

45.77

Weight (kDa)

8.37

Isoelectric Point (pI)

45.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-met PF12874 261 - 285 8.8e-06 Zinc-finger of C2H2 type
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 92
AccB1I GGYRCC 1 cut(s) 806
AccBSI CCGCTC 1 cut(s) 10
AccII CGCG 2 cut(s) 74, 1118
AccIII TCCGGA 1 cut(s) 290
AcoI YGGCCR 3 cut(s) 951, 1035, 1119
AcsI RAATTY 2 cut(s) 563, 1167
AcvI CACGTG 1 cut(s) 168
AcyI GRCGYC 1 cut(s) 1146
AfiI CCNNNNNNNGG 3 cut(s) 344, 345, 454
AgeI ACCGGT 2 cut(s) 63, 117
AgsI TTSAA 4 cut(s) 205, 352, 651, 1141
AleI CACNNNNGTG 1 cut(s) 1181
AluBI AGCT 7 cut(s) 334, 378, 473, 486, 515, 962, 1159
AluI AGCT 7 cut(s) 334, 378, 473, 486, 515, 962, 1159
Alw21I GWGCWC 2 cut(s) 475, 488
Alw26I GTCTC 1 cut(s) 837
Ama87I CYCGRG 1 cut(s) 5
Aor13HI TCCGGA 1 cut(s) 290
AoxI GGCC 4 cut(s) 570, 951, 1035, 1119
ApeKI GCWGC 4 cut(s) 134, 175, 378, 515
ApoI RAATTY 2 cut(s) 563, 1167
ArsI GACNNNNNNTTYG 2 cut(s) 812, 844
AsiGI ACCGGT 2 cut(s) 63, 117
AspLEI GCGC 4 cut(s) 74, 145, 383, 1048
AspS9I GGNCC 2 cut(s) 61, 570
AsuNHI GCTAGC 1 cut(s) 923
AvaI CYCGRG 1 cut(s) 5
AvaII GGWCC 1 cut(s) 61
BanI GGYRCC 1 cut(s) 806
BanII GRGCYC 3 cut(s) 48, 475, 488
BbrPI CACGTG 1 cut(s) 168
BbsI GAAGAC 1 cut(s) 34
Bbv12I GWGCWC 2 cut(s) 475, 488
BbvI GCAGC 4 cut(s) 121, 187, 365, 502
BccI CCATC 1 cut(s) 1087
BceAI ACGGC 1 cut(s) 966
BclI TGATCA 1 cut(s) 258
BcoDI GTCTC 1 cut(s) 837
BfaI CTAG 4 cut(s) 861, 924, 1008, 1092
BisI GCNGC 8 cut(s) 135, 173, 176, 379, 516, 671, 1035, 1119
BlsI GCNGC 8 cut(s) 136, 174, 177, 380, 517, 672, 1036, 1120
Bme18I GGWCC 1 cut(s) 61
BmeT110I CYCGRG 1 cut(s) 5
BmgT120I GGNCC 2 cut(s) 61, 570
BmiI GGNNCC 4 cut(s) 186, 219, 669, 808
BmsI GCATC 6 cut(s) 66, 220, 920, 1004, 1021, 1090
BmtI GCTAGC 1 cut(s) 927
BpiI GAAGAC 1 cut(s) 34
BplI GAGNNNNNCTC 2 cut(s) 687, 719
BpmI CTGGAG 1 cut(s) 472
Bpu10I CCTNAGC 1 cut(s) 963
BpuEI CTTGAG 1 cut(s) 689
BsaAI YACGTR 1 cut(s) 168
BsaHI GRCGYC 1 cut(s) 1146
BsaJI CCNNGG 2 cut(s) 363, 453
BsaWI WCCGGW 4 cut(s) 63, 117, 181, 290
Bsc4I CCNNNNNNNGG 3 cut(s) 344, 345, 454
Bse118I RCCGGY 2 cut(s) 63, 117
Bse1I ACTGG 3 cut(s) 270, 609, 688
Bse3DI GCAATG 3 cut(s) 499, 855, 990
BseAI TCCGGA 1 cut(s) 290
BseDI CCNNGG 2 cut(s) 363, 453
BseGI GGATG 2 cut(s) 211, 1105
BseLI CCNNNNNNNGG 3 cut(s) 344, 345, 454
BseMI GCAATG 3 cut(s) 499, 855, 990
BseMII CTCAG 5 cut(s) 61, 458, 488, 519, 977
BseNI ACTGG 3 cut(s) 270, 609, 688
BseRI GAGGAG 4 cut(s) 210, 213, 680, 750
BseXI GCAGC 4 cut(s) 121, 187, 365, 502
Bsh1236I CGCG 2 cut(s) 74, 1118
BshFI GGCC 4 cut(s) 572, 953, 1037, 1121
BshNI GGYRCC 1 cut(s) 806
BshTI ACCGGT 2 cut(s) 63, 117
BsiHKAI GWGCWC 2 cut(s) 475, 488
BsiHKCI CYCGRG 1 cut(s) 5
BsiSI CCGG 5 cut(s) 64, 118, 182, 215, 291
BslI CCNNNNNNNGG 3 cut(s) 344, 345, 454
BsmAI GTCTC 1 cut(s) 837
BsmBI CGTCTC 1 cut(s) 837
BsnI GGCC 4 cut(s) 572, 953, 1037, 1121
BsoBI CYCGRG 1 cut(s) 5
Bsp1286I GDGCHC 3 cut(s) 48, 475, 488
Bsp13I TCCGGA 1 cut(s) 290
Bsp143I GATC 3 cut(s) 258, 540, 1152
BspANI GGCC 4 cut(s) 572, 953, 1037, 1121
BspCNI CTCAG 5 cut(s) 60, 459, 487, 520, 976
BspEI TCCGGA 1 cut(s) 290
BspFNI CGCG 2 cut(s) 74, 1118
BspHI TCATGA 1 cut(s) 1149
BspLI GGNNCC 4 cut(s) 186, 219, 669, 808
BspOI GCTAGC 1 cut(s) 927
BspT107I GGYRCC 1 cut(s) 806
BsrBI CCGCTC 1 cut(s) 10
BsrDI GCAATG 3 cut(s) 499, 855, 990
BsrFI RCCGGY 2 cut(s) 63, 117
BsrI ACTGG 3 cut(s) 270, 609, 688
BssAI RCCGGY 2 cut(s) 63, 117
BssECI CCNNGG 2 cut(s) 363, 453
BssMI GATC 3 cut(s) 258, 540, 1152
BssNI GRCGYC 1 cut(s) 1146
BssT1I CCWWGG 2 cut(s) 363, 453
Bst4CI ACNGT 4 cut(s) 319, 437, 548, 1205
Bst6I CTCTTC 1 cut(s) 1101
BstACI GRCGYC 1 cut(s) 1146
BstBAI YACGTR 1 cut(s) 168
BstC8I GCNNGC 3 cut(s) 37, 147, 925
BstDEI CTNAG 6 cut(s) 47, 467, 474, 528, 534, 963
BstF5I GGATG 2 cut(s) 211, 1105
BstFNI CGCG 2 cut(s) 74, 1118
BstHHI GCGC 4 cut(s) 74, 145, 383, 1048
BstKTI GATC 3 cut(s) 261, 543, 1155
BstMAI GTCTC 1 cut(s) 837
BstMBI GATC 3 cut(s) 258, 540, 1152
BstMWI GCNNNNNNNGC 7 cut(s) 140, 387, 458, 676, 959, 1043, 1193
BstUI CGCG 2 cut(s) 74, 1118
BstV1I GCAGC 4 cut(s) 121, 187, 365, 502
BstV2I GAAGAC 1 cut(s) 34
BstXI CCANNNNNNTGG 1 cut(s) 825
BsuRI GGCC 4 cut(s) 572, 953, 1037, 1121
BtgZI GCGATG 1 cut(s) 89
BtsCI GGATG 2 cut(s) 211, 1105
BtsI GCAGTG 3 cut(s) 984, 1068, 1176
BtsIMutI CAGTG 7 cut(s) 324, 616, 695, 984, 1068, 1176, 1210
Cac8I GCNNGC 3 cut(s) 37, 147, 925
CciI TCATGA 1 cut(s) 1149
CfoI GCGC 4 cut(s) 74, 145, 383, 1048
Cfr10I RCCGGY 2 cut(s) 63, 117
Cfr13I GGNCC 2 cut(s) 61, 570
CseI GACGC 3 cut(s) 941, 1124, 1135
CspAI ACCGGT 2 cut(s) 63, 117
CspCI CAANNNNNGTGG 2 cut(s) 973, 1008
CviAII CATG 5 cut(s) 22, 140, 392, 907, 1150
DdeI CTNAG 6 cut(s) 47, 467, 474, 528, 534, 963
DpnI GATC 3 cut(s) 260, 542, 1154
DpnII GATC 3 cut(s) 258, 540, 1152
DrdI GACNNNNNNGTC 1 cut(s) 92
DseDI GACNNNNNNGTC 1 cut(s) 92
EaeI YGGCCR 3 cut(s) 951, 1035, 1119
Eam1104I CTCTTC 1 cut(s) 1101
EarI CTCTTC 1 cut(s) 1101
EciI GGCGGA 1 cut(s) 86
Ecl136II GAGCTC 2 cut(s) 473, 486
Eco130I CCWWGG 2 cut(s) 363, 453
Eco24I GRGCYC 3 cut(s) 48, 475, 488
Eco47I GGWCC 1 cut(s) 61
Eco53kI GAGCTC 2 cut(s) 473, 486
Eco72I CACGTG 1 cut(s) 168
Eco88I CYCGRG 1 cut(s) 5
EcoICRI GAGCTC 2 cut(s) 473, 486
EcoRI GAATTC 1 cut(s) 1167
EcoT14I CCWWGG 2 cut(s) 363, 453
EcoT38I GRGCYC 3 cut(s) 48, 475, 488
ErhI CCWWGG 2 cut(s) 363, 453
Esp3I CGTCTC 1 cut(s) 837
FaeI CATG 5 cut(s) 25, 143, 395, 910, 1153
FatI CATG 5 cut(s) 21, 139, 391, 906, 1149
FauI CCCGC 3 cut(s) 3, 40, 154
FauNDI CATATG 1 cut(s) 837
FbaI TGATCA 1 cut(s) 258
Fnu4HI GCNGC 8 cut(s) 135, 173, 176, 379, 516, 671, 1035, 1119
FokI GGATG 2 cut(s) 198, 1112
FriOI GRGCYC 3 cut(s) 48, 475, 488
Fsp4HI GCNGC 8 cut(s) 135, 173, 176, 379, 516, 671, 1035, 1119
FspBI CTAG 4 cut(s) 861, 924, 1008, 1092
GlaI GCGC 4 cut(s) 73, 144, 382, 1047
GluI GCNGC 8 cut(s) 135, 173, 176, 379, 516, 671, 1035, 1119
GsuI CTGGAG 1 cut(s) 472
HaeIII GGCC 4 cut(s) 572, 953, 1037, 1121
HapII CCGG 5 cut(s) 64, 118, 182, 215, 291
HgaI GACGC 3 cut(s) 941, 1124, 1135
HhaI GCGC 4 cut(s) 74, 145, 383, 1048
Hin1I GRCGYC 1 cut(s) 1146
Hin1II CATG 5 cut(s) 25, 143, 395, 910, 1153
Hin6I GCGC 4 cut(s) 72, 143, 381, 1046
HinP1I GCGC 4 cut(s) 72, 143, 381, 1046
HincII GTYRAC 1 cut(s) 1114
HindII GTYRAC 1 cut(s) 1114
HinfI GANTC 8 cut(s) 93, 126, 294, 421, 865, 973, 1025, 1057
HpaII CCGG 5 cut(s) 64, 118, 182, 215, 291
Hpy166II GTNNAC 1 cut(s) 1114
Hpy188I TCNGA 8 cut(s) 125, 192, 477, 529, 894, 919, 1003, 1030
Hpy188III TCNNGA 5 cut(s) 291, 706, 869, 965, 1150
Hpy8I GTNNAC 1 cut(s) 1114
HpyAV CCTTC 2 cut(s) 35, 115
HpyCH4III ACNGT 4 cut(s) 319, 437, 548, 1205
HpyCH4IV ACGT 2 cut(s) 167, 615
HpyCH4V TGCA 9 cut(s) 326, 401, 497, 518, 634, 899, 995, 1103, 1223
HpyF10VI GCNNNNNNNGC 7 cut(s) 140, 387, 458, 676, 959, 1043, 1193
HpyF3I CTNAG 6 cut(s) 47, 467, 474, 528, 534, 963
HpySE526I ACGT 2 cut(s) 167, 615
Hsp92I GRCGYC 1 cut(s) 1146
Hsp92II CATG 5 cut(s) 25, 143, 395, 910, 1153
HspAI GCGC 4 cut(s) 72, 143, 381, 1046
Kpn2I TCCGGA 1 cut(s) 290
Ksp22I TGATCA 1 cut(s) 258
Kzo9I GATC 3 cut(s) 258, 540, 1152
LmnI GCTCC 5 cut(s) 223, 375, 491, 637, 667
Lsp1109I GCAGC 4 cut(s) 121, 187, 365, 502
LweI GCATC 6 cut(s) 66, 220, 920, 1004, 1021, 1090
MaeI CTAG 4 cut(s) 861, 924, 1008, 1092
MaeII ACGT 2 cut(s) 167, 615
MaeIII GTNAC 2 cut(s) 548, 611
MalI GATC 3 cut(s) 260, 542, 1154
MbiI CCGCTC 1 cut(s) 10
MboI GATC 3 cut(s) 258, 540, 1152
MboII GAAGA 9 cut(s) 39, 550, 764, 859, 950, 982, 1034, 1066, 1118
MhlI GDGCHC 3 cut(s) 48, 475, 488
MluCI AATT 4 cut(s) 563, 801, 1167, 1212
MlyI GAGTC 3 cut(s) 102, 303, 874
MmeI TCCRAC 3 cut(s) 645, 1001, 1085
MroI TCCGGA 1 cut(s) 290
MseI TTAA 3 cut(s) 432, 723, 777
MslI CAYNNNNRTG 3 cut(s) 396, 840, 1181
MspA1I CMGCKG 1 cut(s) 35
MspI CCGG 5 cut(s) 64, 118, 182, 215, 291
MvnI CGCG 2 cut(s) 74, 1118
MwoI GCNNNNNNNGC 7 cut(s) 140, 387, 458, 676, 959, 1043, 1193
NdeI CATATG 1 cut(s) 837
NdeII GATC 3 cut(s) 258, 540, 1152
NheI GCTAGC 1 cut(s) 923
NlaIII CATG 5 cut(s) 25, 143, 395, 910, 1153
NlaIV GGNNCC 4 cut(s) 186, 219, 669, 808
NmuCI GTSAC 1 cut(s) 611
OliI CACNNNNGTG 1 cut(s) 1181
PaeR7I CTCGAG 1 cut(s) 5
PagI TCATGA 1 cut(s) 1149
PfeI GAWTC 5 cut(s) 126, 421, 973, 1025, 1057
PinAI ACCGGT 2 cut(s) 63, 117
PkrI GCNGC 8 cut(s) 136, 174, 177, 380, 517, 672, 1036, 1120
PleI GAGTC 3 cut(s) 101, 302, 873
PmaCI CACGTG 1 cut(s) 168
PmlI CACGTG 1 cut(s) 168
PpsI GAGTC 3 cut(s) 101, 302, 873
Ppu21I YACGTR 1 cut(s) 168
Psp124BI GAGCTC 2 cut(s) 475, 488
PspCI CACGTG 1 cut(s) 168
PspN4I GGNNCC 4 cut(s) 186, 219, 669, 808
PspPI GGNCC 2 cut(s) 61, 570
PspXI VCTCGAGB 1 cut(s) 5
RseI CAYNNNNRTG 3 cut(s) 396, 840, 1181
SacI GAGCTC 2 cut(s) 475, 488
SaqAI TTAA 3 cut(s) 432, 723, 777
SatI GCNGC 8 cut(s) 135, 173, 176, 379, 516, 671, 1035, 1119
Sau3AI GATC 3 cut(s) 258, 540, 1152
Sau96I GGNCC 2 cut(s) 61, 570
SchI GAGTC 3 cut(s) 102, 303, 874
SduI GDGCHC 3 cut(s) 48, 475, 488
SfaNI GCATC 6 cut(s) 66, 220, 920, 1004, 1021, 1090
Sfr274I CTCGAG 1 cut(s) 5
SinI GGWCC 1 cut(s) 61
SlaI CTCGAG 1 cut(s) 5
SmiMI CAYNNNNRTG 3 cut(s) 396, 840, 1181
SmlI CTYRAG 2 cut(s) 5, 704
SmoI CTYRAG 2 cut(s) 5, 704
Sse9I AATT 4 cut(s) 563, 801, 1167, 1212
SspMI CTAG 4 cut(s) 861, 924, 1008, 1092
SstI GAGCTC 2 cut(s) 475, 488
StyI CCWWGG 2 cut(s) 363, 453
TaaI ACNGT 4 cut(s) 319, 437, 548, 1205
TaiI ACGT 2 cut(s) 170, 618
TaqI TCGA 6 cut(s) 6, 84, 108, 156, 1014, 1155
TasI AATT 4 cut(s) 563, 801, 1167, 1212
TauI GCSGC 4 cut(s) 175, 673, 1037, 1121
TfiI GAWTC 5 cut(s) 126, 421, 973, 1025, 1057
Tru1I TTAA 3 cut(s) 432, 723, 777
Tru9I TTAA 3 cut(s) 432, 723, 777
TscAI CASTG 7 cut(s) 324, 616, 695, 991, 1075, 1183, 1210
TseFI GTSAC 1 cut(s) 611
TseI GCWGC 4 cut(s) 134, 175, 378, 515
Tsp45I GTSAC 1 cut(s) 611
TspDTI ATGAA 4 cut(s) 413, 618, 895, 1160
TspRI CASTG 7 cut(s) 324, 616, 695, 991, 1075, 1183, 1210
VpaK11BI GGWCC 1 cut(s) 61
XapI RAATTY 2 cut(s) 563, 1167
XhoI CTCGAG 1 cut(s) 5
XspI CTAG 4 cut(s) 861, 924, 1008, 1092
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.