pycom02g00670

Methyladenine glycosylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0015324
Physical Location & Seq
Forward (+)
365075 .. 368210
3136 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g00670.3

Sequence Viewer

Length: 1137 bp
ATGTCAGGAGCTCCAAGAGTAAGGTCTATCAATGTGGCTGATTCAGAGTCAAGGCCAGTGCTTGGACCTGCTGGGAACAAGGCAGGGACTTTCAGTTCGAGAAAACCGGCTTCAAAGCCACTGAGAAAGGCTGAGAAATTGGGTAAGGAGGTTAAATCAGCTGAAGAGAAGAAAACCCGTCAATCTTCAATGCATACTACCTCTCCTCAACCGCATTCTCCCAAGGTTCATTCAGTGCTTCGTCGGCACGAGCAGCTATTGCACTCTAATCTTTCGCTAAATGCATCTTGTTCATCGGATGCCTCTACGGATTCGTTTCATAGTAGATCATCCACGGGTAGGGTGATTCGGTCAAATAGTGTAGGGAGTGGGAGGAAGCAGTATGTTTCAAAGCCGAGAAGTGTTGTTTCTGATGGTGGACTGGACTCTCCTCTTGACGGTTCACAGTCTAAGAAGAGATGCACTTGGGTGACGCCAAATACTGATCCATGTTATGCTGCTTTTCATGATGAAGAATGGGGGCTTCCAGTACACGATGATAAGAAACTGTTTGAGCTTCTGGTCCTATCAGGTGCTTTGGCTGAGCTTTCATGGCCTGCCATTTTAAGCAAAAAGCACATCTTCAGGGAAGTTTTTGCACATTTTGATCCCATTGCCGTTTCAAAATTGAATGAGAAGAAGCTAATAGCACCAGGAAGTGCTGCAAGTTCCCTATTGTCTGAACCGAAGTTGCGTGCTGTCATTGAGAATGCACGTCAAACAACCAAGGTCATAGAGGAGTTTGGGTCATTTGACAAGTATATCTGGAGCTTTGTGAATAACAAGCCCATCGTCAGCCGATTCCGGTATCCTCGGCAGGTTCCGGCAAAAACTCCAAAAGCAGACGTCATAAGCAAGGACCTTGTGAGAAGAGGGTTCCGGAGTGTGGGGCCCACTGTCGTTTACTCTTTCATGCAGGTTGCCGGAATCACAAACGACCATCTCATTGGTTGCTTCAGATTCCAGGAGTGCGTAAACGCTGCGGAAGGGAAGGGAGAGAACGGCACCAAGGATGAGGCTGGAAAGAAAACGGAGAACGGGATAGAATCGGAGTTATCCGTCGCCATAGACAAGTTGAGTTTCTCGTCGGACTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03410 map03410
Pfam Domains
Protein Families
Adenine_glyco

Protein Analysis

379

Amino Acids

41.42

Weight (kDa)

9.6

Isoelectric Point (pI)

51.43

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015324)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G57970 AT5G57970
fragaria_vesca FvH4_1g00950 FvH4_1g00950
malus_domestica MD02G1007800.v1.1
prunus_persica Prupe.7G262700_v2.0.a1
pyrus_communis pycom02g00670 pycom15g13660
rosa_chinensis RchiOBHm_Chr2g0085681
rosa_laevigata RLG00000015711
rosa_multiflora Rmu_sc0007631.1_g000006
rosa_roxburghii Rroxscaffold_2G00155130
rosa_rugosa Rorug01G0462200
rosa_samantha Rh2BG011900 Rh2CG012700
rosa_wichuraiana Rw2G001010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 888
Acc36I ACCTGC 3 cut(s) 76, 847, 946
AccB1I GGYRCC 1 cut(s) 1043
AccB7I CCANNNNNTGG 1 cut(s) 62
AccIII TCCGGA 1 cut(s) 918
AciI CCGC 2 cut(s) 212, 1022
AclWI GGATC 2 cut(s) 479, 641
AcuI CTGAAG 3 cut(s) 183, 607, 979
AcyI GRCGYC 2 cut(s) 473, 885
AfaI GTAC 1 cut(s) 531
AfiI CCNNNNNNNGG 4 cut(s) 62, 339, 437, 925
AgsI TTSAA 5 cut(s) 114, 189, 390, 663, 670
AjiI CACGTC 1 cut(s) 755
AjnI CCWGG 2 cut(s) 691, 1002
AleI CACNNNNGTG 1 cut(s) 467
AloI GAACNNNNNNTCC 2 cut(s) 79, 111
AluBI AGCT 7 cut(s) 11, 161, 256, 556, 586, 682, 810
AluI AGCT 7 cut(s) 11, 161, 256, 556, 586, 682, 810
Alw21I GWGCWC 1 cut(s) 13
AlwI GGATC 2 cut(s) 479, 641
Aor13HI TCCGGA 1 cut(s) 918
AoxI GGCC 3 cut(s) 53, 593, 929
ApaI GGGCCC 1 cut(s) 933
ApeKI GCWGC 4 cut(s) 253, 497, 701, 1019
ArsI GACNNNNNNTTYG 2 cut(s) 79, 111
AspS9I GGNCC 5 cut(s) 65, 562, 898, 929, 930
AsuHPI GGTGA 2 cut(s) 355, 481
AvaII GGWCC 3 cut(s) 65, 562, 898
BaeGI GKGCMC 1 cut(s) 933
BanI GGYRCC 1 cut(s) 1043
BanII GRGCYC 2 cut(s) 13, 933
BauI CACGAG 1 cut(s) 248
Bbv12I GWGCWC 1 cut(s) 13
BbvI GCAGC 4 cut(s) 265, 484, 688, 1006
BccI CCATC 3 cut(s) 407, 836, 987
BceAI ACGGC 2 cut(s) 641, 1057
BciT130I CCWGG 2 cut(s) 693, 1004
BciVI GTATCC 1 cut(s) 858
BfuAI ACCTGC 3 cut(s) 76, 847, 946
BfuI GTATCC 1 cut(s) 858
BisI GCNGC 4 cut(s) 254, 498, 702, 1020
BlpI GCTNAGC 1 cut(s) 582
BlsI GCNGC 4 cut(s) 255, 499, 703, 1021
Bme1390I CCNGG 2 cut(s) 693, 1004
Bme18I GGWCC 3 cut(s) 65, 562, 898
BmgBI CACGTC 1 cut(s) 755
BmgT120I GGNCC 5 cut(s) 65, 562, 898, 929, 930
BmiI GGNNCC 5 cut(s) 861, 917, 930, 931, 1045
BmrFI CCNGG 2 cut(s) 693, 1004
BmsI GCATC 3 cut(s) 289, 293, 449
BpmI CTGGAG 1 cut(s) 826
Bpu1102I GCTNAGC 1 cut(s) 582
BsaHI GRCGYC 2 cut(s) 473, 885
BsaJI CCNNGG 5 cut(s) 222, 333, 765, 851, 1047
BsaWI WCCGGW 2 cut(s) 843, 918
BsaXI ACNNNNNCTCC 4 cut(s) 187, 217, 770, 800
Bsc4I CCNNNNNNNGG 4 cut(s) 62, 339, 437, 925
Bse118I RCCGGY 1 cut(s) 106
Bse1I ACTGG 3 cut(s) 56, 426, 527
Bse3DI GCAATG 1 cut(s) 651
BseAI TCCGGA 1 cut(s) 918
BseBI CCWGG 2 cut(s) 693, 1004
BseDI CCNNGG 5 cut(s) 222, 333, 765, 851, 1047
BseGI GGATG 3 cut(s) 304, 329, 1057
BseLI CCNNNNNNNGG 4 cut(s) 62, 339, 437, 925
BseMI GCAATG 1 cut(s) 651
BseMII CTCAG 3 cut(s) 113, 123, 573
BseNI ACTGG 3 cut(s) 56, 426, 527
BseRI GAGGAG 3 cut(s) 195, 420, 791
BseSI GKGCMC 1 cut(s) 933
BseXI GCAGC 4 cut(s) 265, 484, 688, 1006
BseYI CCCAGC 1 cut(s) 71
BshFI GGCC 3 cut(s) 55, 595, 931
BshNI GGYRCC 1 cut(s) 1043
BsiHKAI GWGCWC 1 cut(s) 13
BsiSI CCGG 5 cut(s) 107, 844, 863, 919, 963
BslFI GGGAC 1 cut(s) 100
BslI CCNNNNNNNGG 4 cut(s) 62, 339, 437, 925
BsmFI GGGAC 1 cut(s) 100
BsmI GAATGC 2 cut(s) 214, 754
BsnI GGCC 3 cut(s) 55, 595, 931
Bsp120I GGGCCC 1 cut(s) 929
Bsp1286I GDGCHC 2 cut(s) 13, 933
Bsp13I TCCGGA 1 cut(s) 918
Bsp143I GATC 3 cut(s) 326, 484, 646
Bsp1720I GCTNAGC 1 cut(s) 582
BspACI CCGC 2 cut(s) 212, 1022
BspANI GGCC 3 cut(s) 55, 595, 931
BspCNI CTCAG 3 cut(s) 114, 124, 574
BspEI TCCGGA 1 cut(s) 918
BspHI TCATGA 1 cut(s) 505
BspLI GGNNCC 5 cut(s) 861, 917, 930, 931, 1045
BspMI ACCTGC 3 cut(s) 76, 847, 946
BspPI GGATC 2 cut(s) 479, 641
BspT107I GGYRCC 1 cut(s) 1043
BsrDI GCAATG 1 cut(s) 651
BsrFI RCCGGY 1 cut(s) 106
BsrI ACTGG 3 cut(s) 56, 426, 527
BssAI RCCGGY 1 cut(s) 106
BssECI CCNNGG 5 cut(s) 222, 333, 765, 851, 1047
BssMI GATC 3 cut(s) 326, 484, 646
BssNI GRCGYC 2 cut(s) 473, 885
BssSI CACGAG 1 cut(s) 248
BssT1I CCWWGG 3 cut(s) 222, 765, 1047
Bst2BI CACGAG 1 cut(s) 248
Bst2UI CCWGG 2 cut(s) 693, 1004
Bst4CI ACNGT 4 cut(s) 440, 447, 549, 937
Bst6I CTCTTC 3 cut(s) 159, 449, 904
BstACI GRCGYC 2 cut(s) 473, 885
BstAPI GCANNNNNTGC 1 cut(s) 259
BstC8I GCNNGC 2 cut(s) 597, 735
BstDEI CTNAG 4 cut(s) 122, 132, 450, 582
BstDSI CCRYGG 1 cut(s) 333
BstF5I GGATG 3 cut(s) 304, 329, 1057
BstKTI GATC 3 cut(s) 329, 487, 649
BstMBI GATC 3 cut(s) 326, 484, 646
BstMWI GCNNNNNNNGC 4 cut(s) 244, 253, 259, 592
BstNI CCWGG 2 cut(s) 693, 1004
BstSCI CCNGG 2 cut(s) 691, 1002
BstSLI GKGCMC 1 cut(s) 933
BstV1I GCAGC 4 cut(s) 265, 484, 688, 1006
BstXI CCANNNNNNTGG 1 cut(s) 986
BsuI GTATCC 1 cut(s) 858
BsuRI GGCC 3 cut(s) 55, 595, 931
BtgI CCRYGG 1 cut(s) 333
BtrI CACGTC 1 cut(s) 755
BtsCI GGATG 3 cut(s) 304, 329, 1057
BtsIMutI CAGTG 4 cut(s) 63, 119, 240, 933
BveI ACCTGC 3 cut(s) 76, 847, 946
Cac8I GCNNGC 2 cut(s) 597, 735
CciI TCATGA 1 cut(s) 505
Cfr10I RCCGGY 1 cut(s) 106
Cfr13I GGNCC 5 cut(s) 65, 562, 898, 929, 930
CseI GACGC 1 cut(s) 481
Csp6I GTAC 1 cut(s) 530
CviAII CATG 4 cut(s) 489, 506, 591, 952
CviQI GTAC 1 cut(s) 530
DdeI CTNAG 4 cut(s) 122, 132, 450, 582
DpnI GATC 3 cut(s) 328, 486, 648
DpnII GATC 3 cut(s) 326, 484, 646
Eam1104I CTCTTC 3 cut(s) 159, 449, 904
EarI CTCTTC 3 cut(s) 159, 449, 904
Ecl136II GAGCTC 1 cut(s) 11
Eco130I CCWWGG 3 cut(s) 222, 765, 1047
Eco24I GRGCYC 2 cut(s) 13, 933
Eco47I GGWCC 3 cut(s) 65, 562, 898
Eco53kI GAGCTC 1 cut(s) 11
Eco57I CTGAAG 3 cut(s) 183, 607, 979
EcoICRI GAGCTC 1 cut(s) 11
EcoO109I RGGNCCY 2 cut(s) 898, 929
EcoRII CCWGG 2 cut(s) 691, 1002
EcoT14I CCWWGG 3 cut(s) 222, 765, 1047
EcoT22I ATGCAT 2 cut(s) 195, 286
EcoT38I GRGCYC 2 cut(s) 13, 933
ErhI CCWWGG 3 cut(s) 222, 765, 1047
FaeI CATG 4 cut(s) 492, 509, 594, 955
FalI AAGNNNNNCTT 2 cut(s) 605, 637
FaqI GGGAC 1 cut(s) 100
FatI CATG 4 cut(s) 488, 505, 590, 951
Fnu4HI GCNGC 4 cut(s) 254, 498, 702, 1020
FokI GGATG 3 cut(s) 311, 316, 1064
FriOI GRGCYC 2 cut(s) 13, 933
Fsp4HI GCNGC 4 cut(s) 254, 498, 702, 1020
GluI GCNGC 4 cut(s) 254, 498, 702, 1020
GsaI CCCAGC 1 cut(s) 75
GsuI CTGGAG 1 cut(s) 826
HaeIII GGCC 3 cut(s) 55, 595, 931
HapII CCGG 5 cut(s) 107, 844, 863, 919, 963
HgaI GACGC 1 cut(s) 481
Hin1I GRCGYC 2 cut(s) 473, 885
Hin1II CATG 4 cut(s) 492, 509, 594, 955
HinfI GANTC 9 cut(s) 41, 47, 311, 346, 425, 840, 966, 999, 1085
HpaII CCGG 5 cut(s) 107, 844, 863, 919, 963
HphI GGTGA 2 cut(s) 355, 481
Hpy166II GTNNAC 5 cut(s) 419, 443, 532, 943, 1015
Hpy188I TCNGA 7 cut(s) 46, 298, 412, 721, 998, 1090, 1129
Hpy188III TCNNGA 6 cut(s) 6, 99, 434, 506, 805, 919
Hpy8I GTNNAC 5 cut(s) 419, 443, 532, 943, 1015
Hpy99I CGWCG 3 cut(s) 246, 1103, 1129
HpyAV CCTTC 2 cut(s) 1019, 1024
HpyCH4III ACNGT 4 cut(s) 440, 447, 549, 937
HpyCH4IV ACGT 2 cut(s) 754, 885
HpyCH4V TGCA 8 cut(s) 193, 262, 284, 462, 638, 704, 752, 955
HpyF10VI GCNNNNNNNGC 4 cut(s) 244, 253, 259, 592
HpyF3I CTNAG 4 cut(s) 122, 132, 450, 582
HpySE526I ACGT 2 cut(s) 754, 885
Hsp92I GRCGYC 2 cut(s) 473, 885
Hsp92II CATG 4 cut(s) 492, 509, 594, 955
Kpn2I TCCGGA 1 cut(s) 918
Kzo9I GATC 3 cut(s) 326, 484, 646
LmnI GCTCC 3 cut(s) 8, 16, 807
Lsp1109I GCAGC 4 cut(s) 265, 484, 688, 1006
LweI GCATC 3 cut(s) 289, 293, 449
MaeII ACGT 2 cut(s) 754, 885
MaeIII GTNAC 1 cut(s) 469
MalI GATC 3 cut(s) 328, 486, 648
MboI GATC 3 cut(s) 326, 484, 646
MboII GAAGA 8 cut(s) 176, 177, 181, 466, 524, 613, 688, 921
MhlI GDGCHC 2 cut(s) 13, 933
MluCI AATT 2 cut(s) 137, 665
MlyI GAGTC 2 cut(s) 56, 419
MmeI TCCRAC 1 cut(s) 1107
Mph1103I ATGCAT 2 cut(s) 195, 286
MroI TCCGGA 1 cut(s) 918
MseI TTAA 2 cut(s) 153, 605
MslI CAYNNNNRTG 1 cut(s) 467
MspA1I CMGCKG 1 cut(s) 161
MspI CCGG 5 cut(s) 107, 844, 863, 919, 963
MspR9I CCNGG 2 cut(s) 693, 1004
Mva1269I GAATGC 2 cut(s) 214, 754
MvaI CCWGG 2 cut(s) 693, 1004
MwoI GCNNNNNNNGC 4 cut(s) 244, 253, 259, 592
NdeII GATC 3 cut(s) 326, 484, 646
NlaIII CATG 4 cut(s) 492, 509, 594, 955
NlaIV GGNNCC 5 cut(s) 861, 917, 930, 931, 1045
NmeAIII GCCGAG 2 cut(s) 420, 832
NmuCI GTSAC 1 cut(s) 469
NsiI ATGCAT 2 cut(s) 195, 286
OliI CACNNNNGTG 1 cut(s) 467
PagI TCATGA 1 cut(s) 505
PctI GAATGC 2 cut(s) 214, 754
PfeI GAWTC 7 cut(s) 41, 311, 346, 840, 966, 999, 1085
PflMI CCANNNNNTGG 1 cut(s) 62
PfoI TCCNGGA 1 cut(s) 1002
PkrI GCNGC 4 cut(s) 255, 499, 703, 1021
PleI GAGTC 2 cut(s) 55, 419
PpsI GAGTC 2 cut(s) 55, 419
PpuMI RGGWCCY 1 cut(s) 898
Psp124BI GAGCTC 1 cut(s) 13
Psp5II RGGWCCY 1 cut(s) 898
Psp6I CCWGG 2 cut(s) 691, 1002
PspFI CCCAGC 1 cut(s) 71
PspGI CCWGG 2 cut(s) 691, 1002
PspN4I GGNNCC 5 cut(s) 861, 917, 930, 931, 1045
PspOMI GGGCCC 1 cut(s) 929
PspPI GGNCC 5 cut(s) 65, 562, 898, 929, 930
PspPPI RGGWCCY 1 cut(s) 898
PvuII CAGCTG 1 cut(s) 161
RsaI GTAC 1 cut(s) 531
RsaNI GTAC 1 cut(s) 530
RseI CAYNNNNRTG 1 cut(s) 467
SacI GAGCTC 1 cut(s) 13
SaqAI TTAA 2 cut(s) 153, 605
SatI GCNGC 4 cut(s) 254, 498, 702, 1020
Sau3AI GATC 3 cut(s) 326, 484, 646
Sau96I GGNCC 5 cut(s) 65, 562, 898, 929, 930
SchI GAGTC 2 cut(s) 56, 419
ScrFI CCNGG 2 cut(s) 693, 1004
SduI GDGCHC 2 cut(s) 13, 933
SfaNI GCATC 3 cut(s) 289, 293, 449
SinI GGWCC 3 cut(s) 65, 562, 898
SmiMI CAYNNNNRTG 1 cut(s) 467
Sse9I AATT 2 cut(s) 137, 665
SsiI CCGC 2 cut(s) 212, 1022
SstI GAGCTC 1 cut(s) 13
StyD4I CCNGG 2 cut(s) 691, 1002
StyI CCWWGG 3 cut(s) 222, 765, 1047
TaaI ACNGT 4 cut(s) 440, 447, 549, 937
TaiI ACGT 2 cut(s) 757, 888
TaqI TCGA 1 cut(s) 98
TaqII GACCGA 1 cut(s) 339
TasI AATT 2 cut(s) 137, 665
TatI WGTACW 1 cut(s) 529
TfiI GAWTC 7 cut(s) 41, 311, 346, 840, 966, 999, 1085
Tru1I TTAA 2 cut(s) 153, 605
Tru9I TTAA 2 cut(s) 153, 605
TscAI CASTG 4 cut(s) 63, 126, 240, 940
TseFI GTSAC 1 cut(s) 469
TseI GCWGC 4 cut(s) 253, 497, 701, 1019
Tsp45I GTSAC 1 cut(s) 469
TspDTI ATGAA 7 cut(s) 218, 282, 308, 494, 525, 579, 940
TspGWI ACGGA 3 cut(s) 323, 1085, 1087
TspRI CASTG 4 cut(s) 63, 126, 240, 940
Van91I CCANNNNNTGG 1 cut(s) 62
VpaK11BI GGWCC 3 cut(s) 65, 562, 898
ZraI GACGTC 1 cut(s) 886
Zsp2I ATGCAT 2 cut(s) 195, 286
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.