Rh2BG011900

Methyladenine glycosylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0015324
Physical Location & Seq
Forward (+)
917259 .. 921329
4071 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG011900.1

Sequence Viewer

Length: 1134 bp
ATGTCAGGAGCTCCAAGAGTAAAGTCTATCAATGTGGCCAATTCTGAGTCACGGTCCGTGCTTGGACCTGCTGGGAACAAAGGAGGGGCATTCAGTGCACGAAAACCAGCTACAAAGCCTCTGAGAAAGACTGAGAAAATGGTTGAGAAGGTTACATCAGCTGAAGACAAGAAAACCCAGCAATCGCTTTCTACCTCTCCTCAGTTGCATTCTCTCAGTGTTCCTTCAGTACTTCGTCGGCACGAGCAGTTATTGCAGTCTAATTTTTCACTAAATGCATCATGTTCATCTGATGCCTCTACGGATTCATTCCATAGTAGAGCATCTACTGGTAGACTGATTCGATCAAATAGTGTAGGGAGTAGGAGGAAGCAGTATGTTTCAAAACCAAGAAGTGTTGTTTCTGATGGTGGATTGGACTCTCCTCCTGGTGGTTCACAGTCAAAGAAACGGTGTGCTTGGGTGACGCCAAATACTGATCCATGTTATGTTGCTTTTCATGATGAAGAATGGGGGCTTCCGGTACATGATGACAAGAGACTGTTTGAGCTTCTTGTCTTATCTGGTGCTTTGGCTGAACTTTCATGGCCTCTCATTCTAAGCAAAAGGCACATCTTTAGGGAAGTGTTTGCAGATTTTGATCCTGTTGCCGTCGCAAAGTTGAATGAGAAGAAGTTAATGGCACCGGGAAGTGTTGCAAGCTCCTCCCTATTGTCTGAGTCAAAGTTGCGTGCTATCCTTGAAAATGCACGTCAAATGACCAAGGTCATAGATGAGTTTGGATCGTTCGACAAGTATATTTGGAGTTTTGTGAATAACAAGCCTATAGTTAGCAGATTCCGGTACCCTCGGCAGGTTCCGGCCAAAACCCCAAAAGCAGATGTGATAAGCAAAGATCTAGTGAGAAGGGGATTTCGTAGTGTGGGTCCCACTGTCATATACTCTTTCATGCAAGTGGCCGGAATTACAAATGACCATCTGGTTAGTTGCTTCAGATTCCAGGAGTGTCTAAACGCCGCAGAAGGGAAGGAAGAGAATGCCACCAAGGACGAGGCTGGAAAGAAAACCGAGAACGGGATAGAATCAGACTTGTCAGTTGCCCTGGACGAGTTGAGTTTCTCCTCAGACTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03410 map03410
Pfam Domains
Protein Families
Adenine_glyco

Protein Analysis

377

Amino Acids

41.41

Weight (kDa)

9.44

Isoelectric Point (pI)

53.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Adenine_glyco PF03352 158 - 332 9.4e-66 Methyladenine glycosylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015324)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G57970 AT5G57970
fragaria_vesca FvH4_1g00950 FvH4_1g00950
malus_domestica MD02G1007800.v1.1
prunus_persica Prupe.7G262700_v2.0.a1
pyrus_communis pycom02g00670 pycom15g13660
rosa_chinensis RchiOBHm_Chr2g0085681
rosa_laevigata RLG00000015711
rosa_multiflora Rmu_sc0007631.1_g000006
rosa_roxburghii Rroxscaffold_2G00155130
rosa_rugosa Rorug01G0462200
rosa_samantha Rh2BG011900 Rh2CG012700
rosa_wichuraiana Rw2G001010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1132
Acc36I ACCTGC 2 cut(s) 76, 844
Acc65I GGTACC 1 cut(s) 843
AccB1I GGYRCC 2 cut(s) 682, 843
AccI GTMKAC 1 cut(s) 334
AciI CCGC 1 cut(s) 1017
AclWI GGATC 3 cut(s) 473, 635, 790
AcoI YGGCCR 3 cut(s) 36, 861, 957
AcuI CTGAAG 3 cut(s) 183, 210, 976
AcyI GRCGYC 1 cut(s) 467
AfaI GTAC 3 cut(s) 231, 525, 845
AfiI CCNNNNNNNGG 4 cut(s) 431, 853, 1023, 1074
AgsI TTSAA 3 cut(s) 384, 664, 743
AjiI CACGTC 1 cut(s) 752
AjnI CCWGG 3 cut(s) 427, 999, 1101
AluBI AGCT 5 cut(s) 11, 110, 161, 550, 702
AluI AGCT 5 cut(s) 11, 110, 161, 550, 702
Alw21I GWGCWC 2 cut(s) 13, 100
Alw26I GTCTC 1 cut(s) 532
Alw44I GTGCAC 1 cut(s) 96
AlwI GGATC 3 cut(s) 473, 635, 790
AoxI GGCC 4 cut(s) 36, 587, 861, 957
ApaLI GTGCAC 1 cut(s) 96
Asp718I GGTACC 1 cut(s) 843
AspS9I GGNCC 3 cut(s) 54, 65, 926
AsuC2I CCSGG 1 cut(s) 687
AsuHPI GGTGA 1 cut(s) 475
AvaII GGWCC 3 cut(s) 54, 65, 926
BaeGI GKGCMC 1 cut(s) 100
BalI TGGCCA 1 cut(s) 38
BanI GGYRCC 2 cut(s) 682, 843
BanII GRGCYC 1 cut(s) 13
BauI CACGAG 1 cut(s) 242
BbsI GAAGAC 1 cut(s) 171
Bbv12I GWGCWC 2 cut(s) 13, 100
BccI CCATC 2 cut(s) 401, 984
BceAI ACGGC 1 cut(s) 635
BciT130I CCWGG 3 cut(s) 429, 1001, 1103
BcnI CCSGG 1 cut(s) 687
BcoDI GTCTC 1 cut(s) 532
BfaI CTAG 1 cut(s) 899
BfmI CTRYAG 1 cut(s) 825
BfuAI ACCTGC 2 cut(s) 76, 844
BglII AGATCT 1 cut(s) 895
BisI GCNGC 1 cut(s) 1017
BlsI GCNGC 1 cut(s) 1018
BmcAI AGTACT 1 cut(s) 231
Bme1390I CCNGG 4 cut(s) 429, 687, 1001, 1103
Bme18I GGWCC 3 cut(s) 54, 65, 926
BmgBI CACGTC 1 cut(s) 752
BmgT120I GGNCC 3 cut(s) 54, 65, 926
BmiI GGNNCC 5 cut(s) 684, 845, 858, 927, 928
BmrFI CCNGG 4 cut(s) 429, 687, 1001, 1103
BmsI GCATC 3 cut(s) 283, 287, 332
BoxI GACNNNNGTC 1 cut(s) 764
BpiI GAAGAC 1 cut(s) 171
BpuMI CCSGG 1 cut(s) 687
BsaBI GATNNNNATC 1 cut(s) 639
BsaHI GRCGYC 1 cut(s) 467
BsaJI CCNNGG 4 cut(s) 762, 848, 1044, 1101
BsaWI WCCGGW 2 cut(s) 520, 840
Bsc4I CCNNNNNNNGG 4 cut(s) 431, 853, 1023, 1074
Bse1I ACTGG 1 cut(s) 334
Bse8I GATNNNNATC 1 cut(s) 639
BseBI CCWGG 3 cut(s) 429, 1001, 1103
BseDI CCNNGG 4 cut(s) 762, 848, 1044, 1101
BseJI GATNNNNATC 1 cut(s) 639
BseLI CCNNNNNNNGG 4 cut(s) 431, 853, 1023, 1074
BseMII CTCAG 6 cut(s) 36, 113, 123, 215, 229, 708
BseNI ACTGG 1 cut(s) 334
BseRI GAGGAG 4 cut(s) 189, 414, 694, 1111
BseSI GKGCMC 1 cut(s) 100
BseYI CCCAGC 2 cut(s) 71, 177
BshFI GGCC 4 cut(s) 38, 589, 863, 959
BshNI GGYRCC 2 cut(s) 682, 843
BsiHKAI GWGCWC 2 cut(s) 13, 100
BsiSI CCGG 5 cut(s) 521, 686, 841, 860, 960
BslFI GGGAC 1 cut(s) 912
BslI CCNNNNNNNGG 4 cut(s) 431, 853, 1023, 1074
BsmAI GTCTC 1 cut(s) 532
BsmFI GGGAC 1 cut(s) 912
BsmI GAATGC 3 cut(s) 89, 208, 1042
BsnI GGCC 4 cut(s) 38, 589, 863, 959
Bsp1286I GDGCHC 2 cut(s) 13, 100
Bsp143I GATC 5 cut(s) 344, 478, 640, 782, 895
BspACI CCGC 1 cut(s) 1017
BspANI GGCC 4 cut(s) 38, 589, 863, 959
BspCNI CTCAG 6 cut(s) 37, 114, 124, 214, 228, 709
BspHI TCATGA 1 cut(s) 499
BspLI GGNNCC 5 cut(s) 684, 845, 858, 927, 928
BspMI ACCTGC 2 cut(s) 76, 844
BspPI GGATC 3 cut(s) 473, 635, 790
BspT107I GGYRCC 2 cut(s) 682, 843
BsrI ACTGG 1 cut(s) 334
BssECI CCNNGG 4 cut(s) 762, 848, 1044, 1101
BssMI GATC 5 cut(s) 344, 478, 640, 782, 895
BssNI GRCGYC 1 cut(s) 467
BssSI CACGAG 1 cut(s) 242
BssT1I CCWWGG 2 cut(s) 762, 1044
Bst2BI CACGAG 1 cut(s) 242
Bst2UI CCWGG 3 cut(s) 429, 1001, 1103
Bst4CI ACNGT 5 cut(s) 54, 441, 453, 543, 934
Bst6I CTCTTC 1 cut(s) 1026
BstACI GRCGYC 1 cut(s) 467
BstAPI GCANNNNNTGC 2 cut(s) 95, 253
BstC8I GCNNGC 2 cut(s) 700, 732
BstDEI CTNAG 8 cut(s) 45, 122, 132, 201, 215, 599, 717, 1123
BstKTI GATC 5 cut(s) 347, 481, 643, 785, 898
BstMAI GTCTC 1 cut(s) 532
BstMBI GATC 5 cut(s) 344, 478, 640, 782, 895
BstMWI GCNNNNNNNGC 2 cut(s) 95, 253
BstNI CCWGG 3 cut(s) 429, 1001, 1103
BstPAI GACNNNNGTC 1 cut(s) 764
BstSCI CCNGG 4 cut(s) 427, 685, 999, 1101
BstSFI CTRYAG 1 cut(s) 825
BstSLI GKGCMC 1 cut(s) 100
BstV2I GAAGAC 1 cut(s) 171
BstX2I RGATCY 1 cut(s) 895
BstYI RGATCY 1 cut(s) 895
BsuRI GGCC 4 cut(s) 38, 589, 863, 959
BtrI CACGTC 1 cut(s) 752
BtsIMutI CAGTG 3 cut(s) 100, 223, 930
BveI ACCTGC 2 cut(s) 76, 844
Cac8I GCNNGC 2 cut(s) 700, 732
CciI TCATGA 1 cut(s) 499
Cfr13I GGNCC 3 cut(s) 54, 65, 926
CpoI CGGWCCG 1 cut(s) 54
CseI GACGC 1 cut(s) 475
Csp6I GTAC 3 cut(s) 230, 524, 844
CspI CGGWCCG 1 cut(s) 54
CviAII CATG 6 cut(s) 282, 483, 500, 527, 585, 949
CviQI GTAC 3 cut(s) 230, 524, 844
DdeI CTNAG 8 cut(s) 45, 122, 132, 201, 215, 599, 717, 1123
DpnI GATC 5 cut(s) 346, 480, 642, 784, 897
DpnII GATC 5 cut(s) 344, 478, 640, 782, 895
EaeI YGGCCR 3 cut(s) 36, 861, 957
Eam1104I CTCTTC 1 cut(s) 1026
EarI CTCTTC 1 cut(s) 1026
Ecl136II GAGCTC 1 cut(s) 11
Eco130I CCWWGG 2 cut(s) 762, 1044
Eco24I GRGCYC 1 cut(s) 13
Eco47I GGWCC 3 cut(s) 54, 65, 926
Eco53kI GAGCTC 1 cut(s) 11
Eco57I CTGAAG 3 cut(s) 183, 210, 976
EcoICRI GAGCTC 1 cut(s) 11
EcoO109I RGGNCCY 1 cut(s) 926
EcoRII CCWGG 3 cut(s) 427, 999, 1101
EcoT14I CCWWGG 2 cut(s) 762, 1044
EcoT22I ATGCAT 1 cut(s) 280
EcoT38I GRGCYC 1 cut(s) 13
ErhI CCWWGG 2 cut(s) 762, 1044
FaeI CATG 6 cut(s) 285, 486, 503, 530, 588, 952
FaqI GGGAC 1 cut(s) 912
FatI CATG 6 cut(s) 281, 482, 499, 526, 584, 948
FblI GTMKAC 1 cut(s) 334
Fnu4HI GCNGC 1 cut(s) 1017
FriOI GRGCYC 1 cut(s) 13
Fsp4HI GCNGC 1 cut(s) 1017
FspBI CTAG 1 cut(s) 899
GluI GCNGC 1 cut(s) 1017
GsaI CCCAGC 2 cut(s) 75, 181
HaeIII GGCC 4 cut(s) 38, 589, 863, 959
HapII CCGG 5 cut(s) 521, 686, 841, 860, 960
HgaI GACGC 1 cut(s) 475
Hin1I GRCGYC 1 cut(s) 467
Hin1II CATG 6 cut(s) 285, 486, 503, 530, 588, 952
HinfI GANTC 8 cut(s) 47, 305, 340, 419, 719, 837, 996, 1082
HpaII CCGG 5 cut(s) 521, 686, 841, 860, 960
HphI GGTGA 1 cut(s) 475
Hpy166II GTNNAC 3 cut(s) 98, 335, 437
Hpy188I TCNGA 8 cut(s) 46, 123, 292, 406, 718, 995, 1087, 1126
Hpy188III TCNNGA 2 cut(s) 6, 500
Hpy8I GTNNAC 3 cut(s) 98, 335, 437
Hpy99I CGWCG 2 cut(s) 240, 656
HpyAV CCTTC 5 cut(s) 142, 234, 900, 1016, 1021
HpyCH4III ACNGT 5 cut(s) 54, 441, 453, 543, 934
HpyCH4IV ACGT 1 cut(s) 751
HpyCH4V TGCA 8 cut(s) 98, 208, 256, 278, 632, 698, 749, 952
HpyF10VI GCNNNNNNNGC 2 cut(s) 95, 253
HpyF3I CTNAG 8 cut(s) 45, 122, 132, 201, 215, 599, 717, 1123
HpySE526I ACGT 1 cut(s) 751
Hsp92I GRCGYC 1 cut(s) 467
Hsp92II CATG 6 cut(s) 285, 486, 503, 530, 588, 952
KflI GGGWCCC 1 cut(s) 926
KpnI GGTACC 1 cut(s) 847
Kzo9I GATC 5 cut(s) 344, 478, 640, 782, 895
LmnI GCTCC 3 cut(s) 8, 16, 707
LweI GCATC 3 cut(s) 283, 287, 332
MaeI CTAG 1 cut(s) 899
MaeII ACGT 1 cut(s) 751
MaeIII GTNAC 3 cut(s) 48, 151, 463
MalI GATC 5 cut(s) 346, 480, 642, 784, 897
MboI GATC 5 cut(s) 344, 478, 640, 782, 895
MboII GAAGA 4 cut(s) 176, 518, 682, 1043
MflI RGATCY 1 cut(s) 895
MhlI GDGCHC 2 cut(s) 13, 100
MlsI TGGCCA 1 cut(s) 38
MluCI AATT 3 cut(s) 40, 262, 963
MluNI TGGCCA 1 cut(s) 38
MlyI GAGTC 3 cut(s) 56, 413, 728
Mox20I TGGCCA 1 cut(s) 38
Mph1103I ATGCAT 1 cut(s) 280
MscI TGGCCA 1 cut(s) 38
MseI TTAA 1 cut(s) 677
MslI CAYNNNNRTG 1 cut(s) 953
Msp20I TGGCCA 1 cut(s) 38
MspA1I CMGCKG 1 cut(s) 161
MspI CCGG 5 cut(s) 521, 686, 841, 860, 960
MspR9I CCNGG 4 cut(s) 429, 687, 1001, 1103
Mva1269I GAATGC 3 cut(s) 89, 208, 1042
MvaI CCWGG 3 cut(s) 429, 1001, 1103
MwoI GCNNNNNNNGC 2 cut(s) 95, 253
NciI CCSGG 1 cut(s) 687
NdeII GATC 5 cut(s) 344, 478, 640, 782, 895
NlaIII CATG 6 cut(s) 285, 486, 503, 530, 588, 952
NlaIV GGNNCC 5 cut(s) 684, 845, 858, 927, 928
NmeAIII GCCGAG 1 cut(s) 829
NmuCI GTSAC 2 cut(s) 48, 463
NsiI ATGCAT 1 cut(s) 280
PagI TCATGA 1 cut(s) 499
PctI GAATGC 3 cut(s) 89, 208, 1042
PfeI GAWTC 5 cut(s) 305, 340, 837, 996, 1082
PfoI TCCNGGA 1 cut(s) 999
PkrI GCNGC 1 cut(s) 1018
PleI GAGTC 3 cut(s) 55, 413, 727
PpsI GAGTC 3 cut(s) 55, 413, 727
PpuMI RGGWCCY 1 cut(s) 926
PshAI GACNNNNGTC 1 cut(s) 764
PsiI TTATAA 1 cut(s) 1132
Psp124BI GAGCTC 1 cut(s) 13
Psp5II RGGWCCY 1 cut(s) 926
Psp6I CCWGG 3 cut(s) 427, 999, 1101
PspFI CCCAGC 2 cut(s) 71, 177
PspGI CCWGG 3 cut(s) 427, 999, 1101
PspN4I GGNNCC 5 cut(s) 684, 845, 858, 927, 928
PspPI GGNCC 3 cut(s) 54, 65, 926
PspPPI RGGWCCY 1 cut(s) 926
PsuI RGATCY 1 cut(s) 895
PvuII CAGCTG 1 cut(s) 161
RsaI GTAC 3 cut(s) 231, 525, 845
RsaNI GTAC 3 cut(s) 230, 524, 844
RseI CAYNNNNRTG 1 cut(s) 953
Rsr2I CGGWCCG 1 cut(s) 54
RsrII CGGWCCG 1 cut(s) 54
SacI GAGCTC 1 cut(s) 13
SaqAI TTAA 1 cut(s) 677
SatI GCNGC 1 cut(s) 1017
Sau3AI GATC 5 cut(s) 344, 478, 640, 782, 895
Sau96I GGNCC 3 cut(s) 54, 65, 926
ScaI AGTACT 1 cut(s) 231
SchI GAGTC 3 cut(s) 56, 413, 728
ScrFI CCNGG 4 cut(s) 429, 687, 1001, 1103
SduI GDGCHC 2 cut(s) 13, 100
SfaNI GCATC 3 cut(s) 283, 287, 332
SfcI CTRYAG 1 cut(s) 825
SinI GGWCC 3 cut(s) 54, 65, 926
SmiMI CAYNNNNRTG 1 cut(s) 953
Sse9I AATT 3 cut(s) 40, 262, 963
SsiI CCGC 1 cut(s) 1017
SspMI CTAG 1 cut(s) 899
SstI GAGCTC 1 cut(s) 13
StyD4I CCNGG 4 cut(s) 427, 685, 999, 1101
StyI CCWWGG 2 cut(s) 762, 1044
TaaI ACNGT 5 cut(s) 54, 441, 453, 543, 934
TaiI ACGT 1 cut(s) 754
TaqI TCGA 2 cut(s) 343, 789
TasI AATT 3 cut(s) 40, 262, 963
TatI WGTACW 1 cut(s) 229
TauI GCSGC 1 cut(s) 1019
TfiI GAWTC 5 cut(s) 305, 340, 837, 996, 1082
Tru1I TTAA 1 cut(s) 677
Tru9I TTAA 1 cut(s) 677
TscAI CASTG 3 cut(s) 100, 223, 937
TseFI GTSAC 2 cut(s) 48, 463
Tsp45I GTSAC 2 cut(s) 48, 463
TspDTI ATGAA 6 cut(s) 276, 297, 488, 519, 573, 937
TspGWI ACGGA 2 cut(s) 46, 317
TspRI CASTG 3 cut(s) 100, 223, 937
VneI GTGCAC 1 cut(s) 96
VpaK11BI GGWCC 3 cut(s) 54, 65, 926
XmiI GTMKAC 1 cut(s) 334
XspI CTAG 1 cut(s) 899
ZrmI AGTACT 1 cut(s) 231
Zsp2I ATGCAT 1 cut(s) 280
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.