RchiOBHm_Chr2g0085681

Methyladenine glycosylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0015324
Physical Location & Seq
Forward (+)
1006810 .. 1011079
4270 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ46123

Sequence Viewer

Length: 1131 bp
ATGTCAGGAGCTCCAAGAGTAAAGTCTATCAATGTGGCCAATTCTGAGTCACGGTCCGTGCTTGGACCTGCTGGGAACAAGGGAGGGGCATTCAGTGCACGAAAACCAGCTACAAAGCCTCTGAGAAAGACTGAGAAAATGGTTGAGGAGGTTACATCAGCTGAAGACAAGAAAACCCAGCAATCGCTTTCTACCTCTCCTCAGTTGCATTCTCTCAGTGTTCCTTCAGTACTTCGTCGGCACGAGCAGTTATTGCAGTCTAATTTTTCACTAAATGCATCATGTTCATCTGATGCCTCTACGGATTCATTCCATAGTAGAGCATCTACTGGTAGACTGATTCGATCAAATAGTGTAGGGAGTAGGAGGAAGCAGTATGTTTCAAAACCAAGAAGTGTTGTTTCTGATGGTGGATTGGACTCTCCTCCTGGTGGTTCACAGTCAAAGAAACGGTGTGCTTGGGTGACGCCAAATACTGATCCATGTTATGTTGCTTTTCATGATGAAGAATGGGGGGTTCCGGTACACGATGACAAGAGACTGTTTGAGCTTCTTGTCTTATCTGGTGCTTTGGCTGAACTTTCATGGCCTCTCATTCTAAGCAAAAAGCACATCTTTAGGGAAGTGTTTGCAGATTTTGATCCTGTTGCCGTCGCAAAGTTGAATGAGAAGAAGTTAATGGCACCGGGAAGTGTTGCAAGCTCCCTATTGTCTGAGTCAAAGTTGCGTGCTATCCTTGAAAATGCACGTCAAATGACCAAGGTCATAGATGAGTTTGGATCGTTCGACAAGTATATTTGGAGTTTTGTGAATAACAAGCCTATAGTTAGCAGATTCCGGTACCCTCGGCAGGTTCCAGCCAAAACCCCAAAAGCAGATGTGATGAGCAAAGATCTAGTGAGAAGGGGATTTCGCAGTGTGGGTCCCACTGTCATATACTCTTTCATGCAAGTCACCGGAATTACAAATGACCATCTGGTTAGTTGCTTCAGATTCCAGGAGTGTCTAAACGCCGCAGAAGGGAAGGAAGAGAATGCCACCAAGGACGAGGCTGGAAAGAAAACCGAGAACGGGATAGAATCAGACTTATCAGTTGCCCTGGACGAGTTGAGTTTCTCCTCAGACTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03410 map03410
Pfam Domains
Protein Families
Adenine_glyco

Protein Analysis

376

Amino Acids

41.33

Weight (kDa)

9.3

Isoelectric Point (pI)

53.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Adenine_glyco PF03352 158 - 331 6.8e-67 Methyladenine glycosylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015324)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G57970 AT5G57970
fragaria_vesca FvH4_1g00950 FvH4_1g00950
malus_domestica MD02G1007800.v1.1
prunus_persica Prupe.7G262700_v2.0.a1
pyrus_communis pycom02g00670 pycom15g13660
rosa_chinensis RchiOBHm_Chr2g0085681
rosa_laevigata RLG00000015711
rosa_multiflora Rmu_sc0007631.1_g000006
rosa_roxburghii Rroxscaffold_2G00155130
rosa_rugosa Rorug01G0462200
rosa_samantha Rh2BG011900 Rh2CG012700
rosa_wichuraiana Rw2G001010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1129
Acc36I ACCTGC 2 cut(s) 76, 841
Acc65I GGTACC 1 cut(s) 840
AccB1I GGYRCC 2 cut(s) 682, 840
AccI GTMKAC 1 cut(s) 334
AciI CCGC 1 cut(s) 1014
AclWI GGATC 3 cut(s) 473, 635, 787
AcoI YGGCCR 1 cut(s) 36
AcuI CTGAAG 3 cut(s) 183, 210, 973
AcyI GRCGYC 1 cut(s) 467
AfaI GTAC 3 cut(s) 231, 525, 842
AfiI CCNNNNNNNGG 4 cut(s) 431, 850, 1020, 1071
AgsI TTSAA 3 cut(s) 384, 664, 740
AjiI CACGTC 1 cut(s) 749
AjnI CCWGG 3 cut(s) 427, 996, 1098
AluBI AGCT 5 cut(s) 11, 110, 161, 550, 702
AluI AGCT 5 cut(s) 11, 110, 161, 550, 702
Alw21I GWGCWC 2 cut(s) 13, 100
Alw26I GTCTC 1 cut(s) 532
Alw44I GTGCAC 1 cut(s) 96
AlwI GGATC 3 cut(s) 473, 635, 787
AoxI GGCC 2 cut(s) 36, 587
ApaLI GTGCAC 1 cut(s) 96
Asp718I GGTACC 1 cut(s) 840
AspS9I GGNCC 3 cut(s) 54, 65, 923
AsuC2I CCSGG 1 cut(s) 687
AsuHPI GGTGA 2 cut(s) 475, 946
AvaII GGWCC 3 cut(s) 54, 65, 923
BaeGI GKGCMC 1 cut(s) 100
BalI TGGCCA 1 cut(s) 38
BanI GGYRCC 2 cut(s) 682, 840
BanII GRGCYC 1 cut(s) 13
BauI CACGAG 1 cut(s) 242
BbsI GAAGAC 1 cut(s) 171
Bbv12I GWGCWC 2 cut(s) 13, 100
BccI CCATC 2 cut(s) 401, 981
BceAI ACGGC 1 cut(s) 635
BciT130I CCWGG 3 cut(s) 429, 998, 1100
BcnI CCSGG 1 cut(s) 687
BcoDI GTCTC 1 cut(s) 532
BfaI CTAG 1 cut(s) 896
BfmI CTRYAG 1 cut(s) 822
BfuAI ACCTGC 2 cut(s) 76, 841
BglII AGATCT 1 cut(s) 892
BisI GCNGC 1 cut(s) 1014
BlsI GCNGC 1 cut(s) 1015
BmcAI AGTACT 1 cut(s) 231
Bme1390I CCNGG 4 cut(s) 429, 687, 998, 1100
Bme18I GGWCC 3 cut(s) 54, 65, 923
BmgBI CACGTC 1 cut(s) 749
BmgT120I GGNCC 3 cut(s) 54, 65, 923
BmiI GGNNCC 6 cut(s) 519, 684, 842, 855, 924, 925
BmrFI CCNGG 4 cut(s) 429, 687, 998, 1100
BmsI GCATC 3 cut(s) 283, 287, 332
BoxI GACNNNNGTC 1 cut(s) 761
BpiI GAAGAC 1 cut(s) 171
BpuMI CCSGG 1 cut(s) 687
BsaBI GATNNNNATC 1 cut(s) 639
BsaHI GRCGYC 1 cut(s) 467
BsaJI CCNNGG 4 cut(s) 759, 845, 1041, 1098
BsaWI WCCGGW 3 cut(s) 520, 837, 956
Bsc4I CCNNNNNNNGG 4 cut(s) 431, 850, 1020, 1071
Bse1I ACTGG 1 cut(s) 334
Bse8I GATNNNNATC 1 cut(s) 639
BseBI CCWGG 3 cut(s) 429, 998, 1100
BseDI CCNNGG 4 cut(s) 759, 845, 1041, 1098
BseJI GATNNNNATC 1 cut(s) 639
BseLI CCNNNNNNNGG 4 cut(s) 431, 850, 1020, 1071
BseMII CTCAG 6 cut(s) 36, 113, 123, 215, 229, 705
BseNI ACTGG 1 cut(s) 334
BseRI GAGGAG 4 cut(s) 161, 189, 414, 1108
BseSI GKGCMC 1 cut(s) 100
BseYI CCCAGC 2 cut(s) 71, 177
BshFI GGCC 2 cut(s) 38, 589
BshNI GGYRCC 2 cut(s) 682, 840
BsiHKAI GWGCWC 2 cut(s) 13, 100
BsiSI CCGG 4 cut(s) 521, 686, 838, 957
BslFI GGGAC 1 cut(s) 909
BslI CCNNNNNNNGG 4 cut(s) 431, 850, 1020, 1071
BsmAI GTCTC 1 cut(s) 532
BsmFI GGGAC 1 cut(s) 909
BsmI GAATGC 3 cut(s) 89, 208, 1039
BsnI GGCC 2 cut(s) 38, 589
Bsp1286I GDGCHC 2 cut(s) 13, 100
Bsp143I GATC 5 cut(s) 344, 478, 640, 779, 892
BspACI CCGC 1 cut(s) 1014
BspANI GGCC 2 cut(s) 38, 589
BspCNI CTCAG 6 cut(s) 37, 114, 124, 214, 228, 706
BspHI TCATGA 1 cut(s) 499
BspLI GGNNCC 6 cut(s) 519, 684, 842, 855, 924, 925
BspMI ACCTGC 2 cut(s) 76, 841
BspPI GGATC 3 cut(s) 473, 635, 787
BspT107I GGYRCC 2 cut(s) 682, 840
BsrI ACTGG 1 cut(s) 334
BssECI CCNNGG 4 cut(s) 759, 845, 1041, 1098
BssMI GATC 5 cut(s) 344, 478, 640, 779, 892
BssNI GRCGYC 1 cut(s) 467
BssSI CACGAG 1 cut(s) 242
BssT1I CCWWGG 2 cut(s) 759, 1041
Bst2BI CACGAG 1 cut(s) 242
Bst2UI CCWGG 3 cut(s) 429, 998, 1100
Bst4CI ACNGT 5 cut(s) 54, 441, 453, 543, 931
Bst6I CTCTTC 1 cut(s) 1023
BstACI GRCGYC 1 cut(s) 467
BstAPI GCANNNNNTGC 2 cut(s) 95, 253
BstC8I GCNNGC 2 cut(s) 700, 729
BstDEI CTNAG 8 cut(s) 45, 122, 132, 201, 215, 599, 714, 1120
BstKTI GATC 5 cut(s) 347, 481, 643, 782, 895
BstMAI GTCTC 1 cut(s) 532
BstMBI GATC 5 cut(s) 344, 478, 640, 779, 892
BstMWI GCNNNNNNNGC 2 cut(s) 95, 253
BstNI CCWGG 3 cut(s) 429, 998, 1100
BstPAI GACNNNNGTC 1 cut(s) 761
BstSCI CCNGG 4 cut(s) 427, 685, 996, 1098
BstSFI CTRYAG 1 cut(s) 822
BstSLI GKGCMC 1 cut(s) 100
BstV2I GAAGAC 1 cut(s) 171
BstX2I RGATCY 1 cut(s) 892
BstYI RGATCY 1 cut(s) 892
BsuRI GGCC 2 cut(s) 38, 589
BtrI CACGTC 1 cut(s) 749
BtsI GCAGTG 1 cut(s) 922
BtsIMutI CAGTG 4 cut(s) 100, 223, 922, 927
BveI ACCTGC 2 cut(s) 76, 841
Cac8I GCNNGC 2 cut(s) 700, 729
CciI TCATGA 1 cut(s) 499
Cfr13I GGNCC 3 cut(s) 54, 65, 923
CpoI CGGWCCG 1 cut(s) 54
CseI GACGC 1 cut(s) 475
Csp6I GTAC 3 cut(s) 230, 524, 841
CspI CGGWCCG 1 cut(s) 54
CviAII CATG 5 cut(s) 282, 483, 500, 585, 946
CviQI GTAC 3 cut(s) 230, 524, 841
DdeI CTNAG 8 cut(s) 45, 122, 132, 201, 215, 599, 714, 1120
DpnI GATC 5 cut(s) 346, 480, 642, 781, 894
DpnII GATC 5 cut(s) 344, 478, 640, 779, 892
EaeI YGGCCR 1 cut(s) 36
Eam1104I CTCTTC 1 cut(s) 1023
EarI CTCTTC 1 cut(s) 1023
Ecl136II GAGCTC 1 cut(s) 11
Eco130I CCWWGG 2 cut(s) 759, 1041
Eco24I GRGCYC 1 cut(s) 13
Eco47I GGWCC 3 cut(s) 54, 65, 923
Eco53kI GAGCTC 1 cut(s) 11
Eco57I CTGAAG 3 cut(s) 183, 210, 973
EcoICRI GAGCTC 1 cut(s) 11
EcoO109I RGGNCCY 1 cut(s) 923
EcoRII CCWGG 3 cut(s) 427, 996, 1098
EcoT14I CCWWGG 2 cut(s) 759, 1041
EcoT22I ATGCAT 1 cut(s) 280
EcoT38I GRGCYC 1 cut(s) 13
ErhI CCWWGG 2 cut(s) 759, 1041
FaeI CATG 5 cut(s) 285, 486, 503, 588, 949
FalI AAGNNNNNCTT 2 cut(s) 599, 631
FaqI GGGAC 1 cut(s) 909
FatI CATG 5 cut(s) 281, 482, 499, 584, 945
FblI GTMKAC 1 cut(s) 334
Fnu4HI GCNGC 1 cut(s) 1014
FriOI GRGCYC 1 cut(s) 13
Fsp4HI GCNGC 1 cut(s) 1014
FspBI CTAG 1 cut(s) 896
GluI GCNGC 1 cut(s) 1014
GsaI CCCAGC 2 cut(s) 75, 181
HaeIII GGCC 2 cut(s) 38, 589
HapII CCGG 4 cut(s) 521, 686, 838, 957
HgaI GACGC 1 cut(s) 475
Hin1I GRCGYC 1 cut(s) 467
Hin1II CATG 5 cut(s) 285, 486, 503, 588, 949
HinfI GANTC 8 cut(s) 47, 305, 340, 419, 716, 834, 993, 1079
HpaII CCGG 4 cut(s) 521, 686, 838, 957
HphI GGTGA 2 cut(s) 475, 946
Hpy166II GTNNAC 4 cut(s) 98, 335, 437, 526
Hpy188I TCNGA 8 cut(s) 46, 123, 292, 406, 715, 992, 1084, 1123
Hpy188III TCNNGA 2 cut(s) 6, 500
Hpy8I GTNNAC 4 cut(s) 98, 335, 437, 526
Hpy99I CGWCG 2 cut(s) 240, 656
HpyAV CCTTC 4 cut(s) 234, 897, 1013, 1018
HpyCH4III ACNGT 5 cut(s) 54, 441, 453, 543, 931
HpyCH4IV ACGT 1 cut(s) 748
HpyCH4V TGCA 8 cut(s) 98, 208, 256, 278, 632, 698, 746, 949
HpyF10VI GCNNNNNNNGC 2 cut(s) 95, 253
HpyF3I CTNAG 8 cut(s) 45, 122, 132, 201, 215, 599, 714, 1120
HpySE526I ACGT 1 cut(s) 748
Hsp92I GRCGYC 1 cut(s) 467
Hsp92II CATG 5 cut(s) 285, 486, 503, 588, 949
KflI GGGWCCC 1 cut(s) 923
KpnI GGTACC 1 cut(s) 844
Kzo9I GATC 5 cut(s) 344, 478, 640, 779, 892
LmnI GCTCC 3 cut(s) 8, 16, 707
LweI GCATC 3 cut(s) 283, 287, 332
MaeI CTAG 1 cut(s) 896
MaeII ACGT 1 cut(s) 748
MaeIII GTNAC 4 cut(s) 48, 151, 463, 952
MalI GATC 5 cut(s) 346, 480, 642, 781, 894
MboI GATC 5 cut(s) 344, 478, 640, 779, 892
MboII GAAGA 4 cut(s) 176, 518, 682, 1040
MflI RGATCY 1 cut(s) 892
MhlI GDGCHC 2 cut(s) 13, 100
MlsI TGGCCA 1 cut(s) 38
MluCI AATT 3 cut(s) 40, 262, 960
MluNI TGGCCA 1 cut(s) 38
MlyI GAGTC 3 cut(s) 56, 413, 725
Mox20I TGGCCA 1 cut(s) 38
Mph1103I ATGCAT 1 cut(s) 280
MscI TGGCCA 1 cut(s) 38
MseI TTAA 1 cut(s) 677
Msp20I TGGCCA 1 cut(s) 38
MspA1I CMGCKG 1 cut(s) 161
MspI CCGG 4 cut(s) 521, 686, 838, 957
MspR9I CCNGG 4 cut(s) 429, 687, 998, 1100
Mva1269I GAATGC 3 cut(s) 89, 208, 1039
MvaI CCWGG 3 cut(s) 429, 998, 1100
MwoI GCNNNNNNNGC 2 cut(s) 95, 253
NciI CCSGG 1 cut(s) 687
NdeII GATC 5 cut(s) 344, 478, 640, 779, 892
NlaIII CATG 5 cut(s) 285, 486, 503, 588, 949
NlaIV GGNNCC 6 cut(s) 519, 684, 842, 855, 924, 925
NmeAIII GCCGAG 1 cut(s) 826
NmuCI GTSAC 3 cut(s) 48, 463, 952
NsiI ATGCAT 1 cut(s) 280
PagI TCATGA 1 cut(s) 499
PctI GAATGC 3 cut(s) 89, 208, 1039
PfeI GAWTC 5 cut(s) 305, 340, 834, 993, 1079
PfoI TCCNGGA 1 cut(s) 996
PkrI GCNGC 1 cut(s) 1015
PleI GAGTC 3 cut(s) 55, 413, 724
PpsI GAGTC 3 cut(s) 55, 413, 724
PpuMI RGGWCCY 1 cut(s) 923
PshAI GACNNNNGTC 1 cut(s) 761
PsiI TTATAA 1 cut(s) 1129
Psp124BI GAGCTC 1 cut(s) 13
Psp5II RGGWCCY 1 cut(s) 923
Psp6I CCWGG 3 cut(s) 427, 996, 1098
PspFI CCCAGC 2 cut(s) 71, 177
PspGI CCWGG 3 cut(s) 427, 996, 1098
PspN4I GGNNCC 6 cut(s) 519, 684, 842, 855, 924, 925
PspPI GGNCC 3 cut(s) 54, 65, 923
PspPPI RGGWCCY 1 cut(s) 923
PsuI RGATCY 1 cut(s) 892
PvuII CAGCTG 1 cut(s) 161
RsaI GTAC 3 cut(s) 231, 525, 842
RsaNI GTAC 3 cut(s) 230, 524, 841
Rsr2I CGGWCCG 1 cut(s) 54
RsrII CGGWCCG 1 cut(s) 54
SacI GAGCTC 1 cut(s) 13
SaqAI TTAA 1 cut(s) 677
SatI GCNGC 1 cut(s) 1014
Sau3AI GATC 5 cut(s) 344, 478, 640, 779, 892
Sau96I GGNCC 3 cut(s) 54, 65, 923
ScaI AGTACT 1 cut(s) 231
SchI GAGTC 3 cut(s) 56, 413, 725
ScrFI CCNGG 4 cut(s) 429, 687, 998, 1100
SduI GDGCHC 2 cut(s) 13, 100
SfaNI GCATC 3 cut(s) 283, 287, 332
SfcI CTRYAG 1 cut(s) 822
SinI GGWCC 3 cut(s) 54, 65, 923
Sse9I AATT 3 cut(s) 40, 262, 960
SsiI CCGC 1 cut(s) 1014
SspMI CTAG 1 cut(s) 896
SstI GAGCTC 1 cut(s) 13
StyD4I CCNGG 4 cut(s) 427, 685, 996, 1098
StyI CCWWGG 2 cut(s) 759, 1041
TaaI ACNGT 5 cut(s) 54, 441, 453, 543, 931
TaiI ACGT 1 cut(s) 751
TaqI TCGA 2 cut(s) 343, 786
TasI AATT 3 cut(s) 40, 262, 960
TatI WGTACW 1 cut(s) 229
TauI GCSGC 1 cut(s) 1016
TfiI GAWTC 5 cut(s) 305, 340, 834, 993, 1079
Tru1I TTAA 1 cut(s) 677
Tru9I TTAA 1 cut(s) 677
TscAI CASTG 4 cut(s) 100, 223, 922, 934
TseFI GTSAC 3 cut(s) 48, 463, 952
Tsp45I GTSAC 3 cut(s) 48, 463, 952
TspDTI ATGAA 6 cut(s) 276, 297, 488, 519, 573, 934
TspGWI ACGGA 2 cut(s) 46, 317
TspRI CASTG 4 cut(s) 100, 223, 922, 934
VneI GTGCAC 1 cut(s) 96
VpaK11BI GGWCC 3 cut(s) 54, 65, 923
XmiI GTMKAC 1 cut(s) 334
XspI CTAG 1 cut(s) 896
ZrmI AGTACT 1 cut(s) 231
Zsp2I ATGCAT 1 cut(s) 280
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.