Rroxscaffold_2G00155130

Methyladenine glycosylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0015324
Physical Location & Seq
Reverse (-)
91521995 .. 91526406
4412 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00155130.1

Sequence Viewer

Length: 1281 bp
ATGTCAGGAGCTCCAAGAGTAAAGTCTATCAATGTGGCCAATTCTGAGTCACGGTCCGTGCTTGGACCTGCTGGGAACAAGGGAGGGGCATTCAGTGCACGAAAACCAGCTACAAAGCCTCTGAGAAAGACTGAGAAAATGGGTGAGAAGGTTACATCAGCTGAAGACAAGAAAACCCAGCAATCGCTTTCTACCTCCCCTCAGTTGCATTCTCTCAGTGTTCCTTCAGTACTTCGTCGGCACGAGCAGTTATTGCAGTCTAATTTTTCACTAAATGCATCGTGTTCATCTGATGCCTCTACGGATTCATTCCATAGTAGAGCATCTACTGGTAGACTGATTCGATCAAATAGTGTAGGGAGTAGGAGGAAGCAGTATGTTTCAAAACCAAGAAGTGTTGTTTCTGATGGTGGATTGGACTCTCCTCCTGGTGGTTCACAGTCAAAGAAACGGTGTGCTTGGGTGACGCCAAATACTGATCCATGTTATGTTGCTTTTCATGATGAAGAATGGGGGCTTCCGGTACATGATGACAAGGAAGTGTTTGCAGATTTTGATCCTGTTACCGTCGCAAAGTTGAATGAGAAGAAGTTAATGGCACCGGGAAGTGTTGCAAGCTCCCTATTGCCTGAGTCAAAGTTGCGTGCTATCCTTGAAAATGCACGTCAAATGACCAAGGTCATAGATGAGTTTGGATCGTTCGACAAGTATATTTGGAGTTTTGTGAATAACAAGCCTATAATTAGCAGATTCCGGTACCCTCGGCAGGTTCCAGCCAAAACCCCAAAAGCAGATGTGATAAGCAAAGATCTAGTGAGAAGGGGATTTCGTAGTGTGGGTCCCACTGTCATATACTCTTTCATGCAAGTCACCGGAATTACAAATGACCATCTGGTTAGTTGCTTCAGATTCCAGGAGTGTCTAAACGCCGCGGAAGGGAAGGAAGAGAATGCCACCAAGGACGAGGCTGAAAAGAAAACCGAGAACGGGATAGGATCAGACTTGTCAGTTGCCCTGGACGAGTTGAGTTTCTCCTCAGACCAGCCACAACTTAAACTGAAAGGTCTATTGGCTTTAAATGGATTGCTGATAATGAAGGATCTGTGCACAATGTCTCCATGTTCCCTTGGGACAGTCGATGCAGACTCCCGATTAGTAGACAATTTTCAGTGTGTGCTCAACATCACCATCAAAAATGTGGGGACATCAAGTATGGATCTAGATGCCGACTGTCTAGGAAGCCTTCTGGCATCTGTACCAAAAGCTTCTTTTTCAAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03410 map03410
Pfam Domains
Protein Families
Adenine_glyco

Protein Analysis

426

Amino Acids

46.32

Weight (kDa)

9.07

Isoelectric Point (pI)

46.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Adenine_glyco PF03352 180 - 303 5e-40 Methyladenine glycosylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015324)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G57970 AT5G57970
fragaria_vesca FvH4_1g00950 FvH4_1g00950
malus_domestica MD02G1007800.v1.1
prunus_persica Prupe.7G262700_v2.0.a1
pyrus_communis pycom02g00670 pycom15g13660
rosa_chinensis RchiOBHm_Chr2g0085681
rosa_laevigata RLG00000015711
rosa_multiflora Rmu_sc0007631.1_g000006
rosa_roxburghii Rroxscaffold_2G00155130
rosa_rugosa Rorug01G0462200
rosa_samantha Rh2BG011900 Rh2CG012700
rosa_wichuraiana Rw2G001010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 76, 757
Acc65I GGTACC 1 cut(s) 756
AccB1I GGYRCC 2 cut(s) 598, 756
AccI GTMKAC 2 cut(s) 334, 1158
AccII CGCG 1 cut(s) 932
AciI CCGC 2 cut(s) 930, 932
AclWI GGATC 6 cut(s) 473, 551, 703, 1003, 1107, 1224
AcoI YGGCCR 1 cut(s) 36
AcuI CTGAAG 3 cut(s) 183, 210, 889
AcyI GRCGYC 1 cut(s) 467
AfaI GTAC 4 cut(s) 231, 525, 758, 1257
AfiI CCNNNNNNNGG 4 cut(s) 431, 766, 936, 987
AgsI TTSAA 4 cut(s) 384, 580, 656, 1275
AjiI CACGTC 1 cut(s) 665
AjnI CCWGG 3 cut(s) 427, 912, 1014
AjuI GAANNNNNNNTTGG 2 cut(s) 1052, 1084
AluBI AGCT 5 cut(s) 11, 110, 161, 618, 1265
AluI AGCT 5 cut(s) 11, 110, 161, 618, 1265
Alw21I GWGCWC 4 cut(s) 13, 100, 1109, 1179
Alw26I GTCTC 1 cut(s) 1119
Alw44I GTGCAC 2 cut(s) 96, 1105
AlwI GGATC 6 cut(s) 473, 551, 703, 1003, 1107, 1224
AoxI GGCC 1 cut(s) 36
ApaLI GTGCAC 2 cut(s) 96, 1105
Asp718I GGTACC 1 cut(s) 756
AspS9I GGNCC 3 cut(s) 54, 65, 839
AsuC2I CCSGG 1 cut(s) 603
AsuHPI GGTGA 4 cut(s) 155, 475, 862, 1177
AvaII GGWCC 3 cut(s) 54, 65, 839
BaeGI GKGCMC 2 cut(s) 100, 1109
BalI TGGCCA 1 cut(s) 38
BanI GGYRCC 2 cut(s) 598, 756
BanII GRGCYC 1 cut(s) 13
BauI CACGAG 1 cut(s) 242
BbsI GAAGAC 1 cut(s) 171
Bbv12I GWGCWC 4 cut(s) 13, 100, 1109, 1179
BccI CCATC 3 cut(s) 401, 897, 1196
BciT130I CCWGG 3 cut(s) 429, 914, 1016
BcnI CCSGG 1 cut(s) 603
BcoDI GTCTC 1 cut(s) 1119
BfaI CTAG 3 cut(s) 812, 1220, 1235
BfuAI ACCTGC 2 cut(s) 76, 757
BglII AGATCT 1 cut(s) 808
BisI GCNGC 1 cut(s) 930
BlsI GCNGC 1 cut(s) 931
BmcAI AGTACT 1 cut(s) 231
Bme1390I CCNGG 4 cut(s) 429, 603, 914, 1016
Bme18I GGWCC 3 cut(s) 54, 65, 839
BmgBI CACGTC 1 cut(s) 665
BmgT120I GGNCC 3 cut(s) 54, 65, 839
BmiI GGNNCC 5 cut(s) 600, 758, 771, 840, 841
BmrFI CCNGG 4 cut(s) 429, 603, 914, 1016
BmsI GCATC 6 cut(s) 283, 287, 332, 1129, 1213, 1259
BoxI GACNNNNGTC 1 cut(s) 677
BpiI GAAGAC 1 cut(s) 171
BpuMI CCSGG 1 cut(s) 603
BsaBI GATNNNNATC 1 cut(s) 555
BsaHI GRCGYC 1 cut(s) 467
BsaJI CCNNGG 6 cut(s) 675, 761, 930, 957, 1014, 1126
BsaWI WCCGGW 3 cut(s) 520, 753, 872
BsaXI ACNNNNNCTCC 2 cut(s) 1099, 1129
Bsc4I CCNNNNNNNGG 4 cut(s) 431, 766, 936, 987
Bse1I ACTGG 1 cut(s) 334
Bse8I GATNNNNATC 1 cut(s) 555
BseBI CCWGG 3 cut(s) 429, 914, 1016
BseDI CCNNGG 6 cut(s) 675, 761, 930, 957, 1014, 1126
BseJI GATNNNNATC 1 cut(s) 555
BseLI CCNNNNNNNGG 4 cut(s) 431, 766, 936, 987
BseMII CTCAG 7 cut(s) 36, 113, 123, 215, 229, 621, 1050
BseNI ACTGG 1 cut(s) 334
BseRI GAGGAG 2 cut(s) 414, 1024
BseSI GKGCMC 2 cut(s) 100, 1109
BseYI CCCAGC 2 cut(s) 71, 177
Bsh1236I CGCG 1 cut(s) 932
BshFI GGCC 1 cut(s) 38
BshNI GGYRCC 2 cut(s) 598, 756
BsiHKAI GWGCWC 4 cut(s) 13, 100, 1109, 1179
BsiSI CCGG 4 cut(s) 521, 602, 754, 873
BslFI GGGAC 3 cut(s) 825, 1144, 1216
BslI CCNNNNNNNGG 4 cut(s) 431, 766, 936, 987
BsmAI GTCTC 1 cut(s) 1119
BsmFI GGGAC 3 cut(s) 825, 1144, 1216
BsmI GAATGC 3 cut(s) 89, 208, 955
BsnI GGCC 1 cut(s) 38
Bsp1286I GDGCHC 4 cut(s) 13, 100, 1109, 1179
Bsp143I GATC 8 cut(s) 344, 478, 556, 695, 808, 995, 1099, 1216
BspACI CCGC 2 cut(s) 930, 932
BspANI GGCC 1 cut(s) 38
BspCNI CTCAG 7 cut(s) 37, 114, 124, 214, 228, 622, 1049
BspFNI CGCG 1 cut(s) 932
BspHI TCATGA 1 cut(s) 499
BspLI GGNNCC 5 cut(s) 600, 758, 771, 840, 841
BspMI ACCTGC 2 cut(s) 76, 757
BspPI GGATC 6 cut(s) 473, 551, 703, 1003, 1107, 1224
BspT107I GGYRCC 2 cut(s) 598, 756
BsrI ACTGG 1 cut(s) 334
BssECI CCNNGG 6 cut(s) 675, 761, 930, 957, 1014, 1126
BssMI GATC 8 cut(s) 344, 478, 556, 695, 808, 995, 1099, 1216
BssNI GRCGYC 1 cut(s) 467
BssSI CACGAG 1 cut(s) 242
BssT1I CCWWGG 3 cut(s) 675, 957, 1126
Bst2BI CACGAG 1 cut(s) 242
Bst2UI CCWGG 3 cut(s) 429, 914, 1016
Bst4CI ACNGT 7 cut(s) 54, 441, 453, 568, 847, 1135, 1232
Bst6I CTCTTC 1 cut(s) 939
BstACI GRCGYC 1 cut(s) 467
BstAPI GCANNNNNTGC 2 cut(s) 95, 253
BstC8I GCNNGC 2 cut(s) 616, 645
BstDEI CTNAG 7 cut(s) 45, 122, 132, 201, 215, 630, 1036
BstDSI CCRYGG 1 cut(s) 930
BstFNI CGCG 1 cut(s) 932
BstKTI GATC 8 cut(s) 347, 481, 559, 698, 811, 998, 1102, 1219
BstMAI GTCTC 1 cut(s) 1119
BstMBI GATC 8 cut(s) 344, 478, 556, 695, 808, 995, 1099, 1216
BstMWI GCNNNNNNNGC 2 cut(s) 95, 253
BstNI CCWGG 3 cut(s) 429, 914, 1016
BstPAI GACNNNNGTC 1 cut(s) 677
BstSCI CCNGG 4 cut(s) 427, 601, 912, 1014
BstSLI GKGCMC 2 cut(s) 100, 1109
BstUI CGCG 1 cut(s) 932
BstV2I GAAGAC 1 cut(s) 171
BstX2I RGATCY 3 cut(s) 808, 1099, 1216
BstYI RGATCY 3 cut(s) 808, 1099, 1216
BsuRI GGCC 1 cut(s) 38
BtgI CCRYGG 1 cut(s) 930
BtrI CACGTC 1 cut(s) 665
BtsIMutI CAGTG 4 cut(s) 100, 223, 843, 1175
BveI ACCTGC 2 cut(s) 76, 757
Cac8I GCNNGC 2 cut(s) 616, 645
CciI TCATGA 1 cut(s) 499
Cfr13I GGNCC 3 cut(s) 54, 65, 839
Cfr42I CCGCGG 1 cut(s) 933
CpoI CGGWCCG 1 cut(s) 54
CseI GACGC 1 cut(s) 475
Csp6I GTAC 4 cut(s) 230, 524, 757, 1256
CspI CGGWCCG 1 cut(s) 54
CviAII CATG 5 cut(s) 483, 500, 527, 862, 1119
CviQI GTAC 4 cut(s) 230, 524, 757, 1256
DdeI CTNAG 7 cut(s) 45, 122, 132, 201, 215, 630, 1036
DpnI GATC 8 cut(s) 346, 480, 558, 697, 810, 997, 1101, 1218
DpnII GATC 8 cut(s) 344, 478, 556, 695, 808, 995, 1099, 1216
DraI TTTAAA 1 cut(s) 1077
EaeI YGGCCR 1 cut(s) 36
Eam1104I CTCTTC 1 cut(s) 939
EarI CTCTTC 1 cut(s) 939
Ecl136II GAGCTC 1 cut(s) 11
Eco130I CCWWGG 3 cut(s) 675, 957, 1126
Eco24I GRGCYC 1 cut(s) 13
Eco47I GGWCC 3 cut(s) 54, 65, 839
Eco53kI GAGCTC 1 cut(s) 11
Eco57I CTGAAG 3 cut(s) 183, 210, 889
EcoICRI GAGCTC 1 cut(s) 11
EcoO109I RGGNCCY 1 cut(s) 839
EcoRII CCWGG 3 cut(s) 427, 912, 1014
EcoT14I CCWWGG 3 cut(s) 675, 957, 1126
EcoT22I ATGCAT 1 cut(s) 280
EcoT38I GRGCYC 1 cut(s) 13
ErhI CCWWGG 3 cut(s) 675, 957, 1126
FaeI CATG 5 cut(s) 486, 503, 530, 865, 1122
FaqI GGGAC 3 cut(s) 825, 1144, 1216
FatI CATG 5 cut(s) 482, 499, 526, 861, 1118
FblI GTMKAC 2 cut(s) 334, 1158
Fnu4HI GCNGC 1 cut(s) 930
FriOI GRGCYC 1 cut(s) 13
Fsp4HI GCNGC 1 cut(s) 930
FspBI CTAG 3 cut(s) 812, 1220, 1235
GluI GCNGC 1 cut(s) 930
GsaI CCCAGC 2 cut(s) 75, 181
HaeIII GGCC 1 cut(s) 38
HapII CCGG 4 cut(s) 521, 602, 754, 873
HgaI GACGC 1 cut(s) 475
Hin1I GRCGYC 1 cut(s) 467
Hin1II CATG 5 cut(s) 486, 503, 530, 865, 1122
HindIII AAGCTT 1 cut(s) 1263
HinfI GANTC 8 cut(s) 47, 305, 340, 419, 632, 750, 909, 1145
HpaII CCGG 4 cut(s) 521, 602, 754, 873
HphI GGTGA 4 cut(s) 155, 475, 862, 1177
Hpy166II GTNNAC 5 cut(s) 98, 335, 437, 1107, 1159
Hpy188I TCNGA 7 cut(s) 46, 123, 292, 406, 908, 1000, 1039
Hpy188III TCNNGA 4 cut(s) 6, 500, 1149, 1220
Hpy8I GTNNAC 5 cut(s) 98, 335, 437, 1107, 1159
Hpy99I CGWCG 2 cut(s) 240, 572
HpyAV CCTTC 7 cut(s) 142, 234, 813, 929, 934, 1090, 1253
HpyCH4III ACNGT 7 cut(s) 54, 441, 453, 568, 847, 1135, 1232
HpyCH4IV ACGT 1 cut(s) 664
HpyF10VI GCNNNNNNNGC 2 cut(s) 95, 253
HpyF3I CTNAG 7 cut(s) 45, 122, 132, 201, 215, 630, 1036
HpySE526I ACGT 1 cut(s) 664
Hsp92I GRCGYC 1 cut(s) 467
Hsp92II CATG 5 cut(s) 486, 503, 530, 865, 1122
KflI GGGWCCC 1 cut(s) 839
KpnI GGTACC 1 cut(s) 760
KspI CCGCGG 1 cut(s) 933
Kzo9I GATC 8 cut(s) 344, 478, 556, 695, 808, 995, 1099, 1216
LmnI GCTCC 3 cut(s) 8, 16, 623
LweI GCATC 6 cut(s) 283, 287, 332, 1129, 1213, 1259
MaeI CTAG 3 cut(s) 812, 1220, 1235
MaeII ACGT 1 cut(s) 664
MaeIII GTNAC 5 cut(s) 48, 151, 463, 562, 868
MalI GATC 8 cut(s) 346, 480, 558, 697, 810, 997, 1101, 1218
MboI GATC 8 cut(s) 344, 478, 556, 695, 808, 995, 1099, 1216
MboII GAAGA 4 cut(s) 176, 518, 598, 956
MflI RGATCY 3 cut(s) 808, 1099, 1216
MhlI GDGCHC 4 cut(s) 13, 100, 1109, 1179
MlsI TGGCCA 1 cut(s) 38
MluCI AATT 5 cut(s) 40, 262, 741, 876, 1162
MluNI TGGCCA 1 cut(s) 38
MlyI GAGTC 4 cut(s) 56, 413, 641, 1139
Mox20I TGGCCA 1 cut(s) 38
Mph1103I ATGCAT 1 cut(s) 280
MscI TGGCCA 1 cut(s) 38
MseI TTAA 3 cut(s) 593, 1053, 1076
Msp20I TGGCCA 1 cut(s) 38
MspA1I CMGCKG 2 cut(s) 161, 932
MspI CCGG 4 cut(s) 521, 602, 754, 873
MspR9I CCNGG 4 cut(s) 429, 603, 914, 1016
Mva1269I GAATGC 3 cut(s) 89, 208, 955
MvaI CCWGG 3 cut(s) 429, 914, 1016
MvnI CGCG 1 cut(s) 932
MwoI GCNNNNNNNGC 2 cut(s) 95, 253
NciI CCSGG 1 cut(s) 603
NdeII GATC 8 cut(s) 344, 478, 556, 695, 808, 995, 1099, 1216
NlaIII CATG 5 cut(s) 486, 503, 530, 865, 1122
NlaIV GGNNCC 5 cut(s) 600, 758, 771, 840, 841
NmeAIII GCCGAG 1 cut(s) 742
NmuCI GTSAC 3 cut(s) 48, 463, 868
NsiI ATGCAT 1 cut(s) 280
PagI TCATGA 1 cut(s) 499
PctI GAATGC 3 cut(s) 89, 208, 955
PfeI GAWTC 4 cut(s) 305, 340, 750, 909
PfoI TCCNGGA 1 cut(s) 912
PkrI GCNGC 1 cut(s) 931
PleI GAGTC 4 cut(s) 55, 413, 640, 1139
PpsI GAGTC 4 cut(s) 55, 413, 640, 1139
PpuMI RGGWCCY 1 cut(s) 839
PshAI GACNNNNGTC 1 cut(s) 677
Psp124BI GAGCTC 1 cut(s) 13
Psp5II RGGWCCY 1 cut(s) 839
Psp6I CCWGG 3 cut(s) 427, 912, 1014
PspFI CCCAGC 2 cut(s) 71, 177
PspGI CCWGG 3 cut(s) 427, 912, 1014
PspN4I GGNNCC 5 cut(s) 600, 758, 771, 840, 841
PspPI GGNCC 3 cut(s) 54, 65, 839
PspPPI RGGWCCY 1 cut(s) 839
PsuI RGATCY 3 cut(s) 808, 1099, 1216
PvuII CAGCTG 1 cut(s) 161
RsaI GTAC 4 cut(s) 231, 525, 758, 1257
RsaNI GTAC 4 cut(s) 230, 524, 757, 1256
Rsr2I CGGWCCG 1 cut(s) 54
RsrII CGGWCCG 1 cut(s) 54
SacI GAGCTC 1 cut(s) 13
SacII CCGCGG 1 cut(s) 933
SaqAI TTAA 3 cut(s) 593, 1053, 1076
SatI GCNGC 1 cut(s) 930
Sau3AI GATC 8 cut(s) 344, 478, 556, 695, 808, 995, 1099, 1216
Sau96I GGNCC 3 cut(s) 54, 65, 839
ScaI AGTACT 1 cut(s) 231
SchI GAGTC 4 cut(s) 56, 413, 641, 1139
ScrFI CCNGG 4 cut(s) 429, 603, 914, 1016
SduI GDGCHC 4 cut(s) 13, 100, 1109, 1179
SfaNI GCATC 6 cut(s) 283, 287, 332, 1129, 1213, 1259
Sfr303I CCGCGG 1 cut(s) 933
SgrBI CCGCGG 1 cut(s) 933
SinI GGWCC 3 cut(s) 54, 65, 839
Sse9I AATT 5 cut(s) 40, 262, 741, 876, 1162
SsiI CCGC 2 cut(s) 930, 932
SspMI CTAG 3 cut(s) 812, 1220, 1235
SstI GAGCTC 1 cut(s) 13
StyD4I CCNGG 4 cut(s) 427, 601, 912, 1014
StyI CCWWGG 3 cut(s) 675, 957, 1126
TaaI ACNGT 7 cut(s) 54, 441, 453, 568, 847, 1135, 1232
TaiI ACGT 1 cut(s) 667
TaqI TCGA 3 cut(s) 343, 702, 1137
TasI AATT 5 cut(s) 40, 262, 741, 876, 1162
TatI WGTACW 1 cut(s) 229
TauI GCSGC 1 cut(s) 932
TfiI GAWTC 4 cut(s) 305, 340, 750, 909
Tru1I TTAA 3 cut(s) 593, 1053, 1076
Tru9I TTAA 3 cut(s) 593, 1053, 1076
TscAI CASTG 4 cut(s) 100, 223, 850, 1175
TseFI GTSAC 3 cut(s) 48, 463, 868
Tsp45I GTSAC 3 cut(s) 48, 463, 868
TspDTI ATGAA 6 cut(s) 276, 297, 488, 519, 850, 1109
TspGWI ACGGA 2 cut(s) 46, 317
TspRI CASTG 4 cut(s) 100, 223, 850, 1175
VneI GTGCAC 2 cut(s) 96, 1105
VpaK11BI GGWCC 3 cut(s) 54, 65, 839
XbaI TCTAGA 1 cut(s) 1219
XcmI CCANNNNNNNNNTGG 1 cut(s) 1195
XmiI GTMKAC 2 cut(s) 334, 1158
XspI CTAG 3 cut(s) 812, 1220, 1235
ZrmI AGTACT 1 cut(s) 231
Zsp2I ATGCAT 1 cut(s) 280
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.