pycom02g05300

pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0009451
Physical Location & Seq
Reverse (-)
3548710 .. 3549688
979 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 720 bp
ATGTCAACATTATTCCAACACATTTTCTATGTTCTTATTCTTCTTCTCCTCCTCTATTCAACCCCATCACAGTGCCATTCGAAGGGACTCCGACCGGGAAATCCGGCCGGAAACGTGCACTTAACGAAGAACATGACTCGAGCCCAATTATCAGAACAGCAATTCATGAAGTGGGTTAAGTTTGTTGGAAGCCTCAAACACTCTGTTTTCAAAGCAGCAAAGAACAAGCTCTCTCCTTCTTTCACTCTGCATGTGGACAAGAATCCTAATGCAGGAGACTTCACAAAAATCCAAGACGCCATTGATTCTCTCCCCTTCATCAATCTCGTGAGAGTTGTTATCAAAGTACATGCCGGAATATACACGGAGAAGGTAAATATACCTCCATTGAAATCATTTATAACCATAGAAGGAGCAGGGGCAGATAAAACCATTGTTCAATGGGGAGACACAGCTCAAACACCATCTGGTCCAAAAAAACAACCCATGGGGACTTTTAATTCTGCAACTTTTGCTGTCAATTCCCCTTATTTTATTGCCAGAAACATTACATTTAAGAACACTACACCAGTCCCCAAACCAGGAGCAATTGGGAAGCAGGCAGTGGCGTTTAGAATATCAGCTGATACAGCAGCTTTCTGGGGGTGTAGTTTTTTGGGGGCACAAGACACACTCTATGATCGATTTGGGAAGGCATTATTACAAGGATTGCTATATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0030312 GO:0044424 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Pectinesterase

Protein Analysis

240

Amino Acids

26.25

Weight (kDa)

9.88

Isoelectric Point (pI)

33.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 90 - 227 4.2e-29 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 401
AcoI YGGCCR 1 cut(s) 105
AcyI GRCGYC 1 cut(s) 297
AfaI GTAC 1 cut(s) 348
AfiI CCNNNNNNNGG 3 cut(s) 82, 272, 581
AgsI TTSAA 4 cut(s) 60, 211, 391, 440
AjnI CCWGG 1 cut(s) 580
AluBI AGCT 4 cut(s) 229, 455, 623, 635
AluI AGCT 4 cut(s) 229, 455, 623, 635
Alw21I GWGCWC 1 cut(s) 120
Alw26I GTCTC 2 cut(s) 270, 441
Alw44I GTGCAC 1 cut(s) 116
Ama87I CYCGRG 1 cut(s) 138
AoxI GGCC 1 cut(s) 105
ApaLI GTGCAC 1 cut(s) 116
ApeKI GCWGC 2 cut(s) 215, 632
AspS9I GGNCC 1 cut(s) 470
AsuC2I CCSGG 1 cut(s) 96
AsuII TTCGAA 1 cut(s) 80
AvaI CYCGRG 1 cut(s) 138
AvaII GGWCC 1 cut(s) 470
BaeGI GKGCMC 2 cut(s) 120, 664
BanII GRGCYC 1 cut(s) 145
BauI CACGAG 1 cut(s) 326
Bbv12I GWGCWC 1 cut(s) 120
BbvI GCAGC 2 cut(s) 227, 644
BccI CCATC 2 cut(s) 73, 472
BciT130I CCWGG 1 cut(s) 582
BcnI CCSGG 1 cut(s) 96
BcoDI GTCTC 2 cut(s) 270, 441
BisI GCNGC 2 cut(s) 216, 633
BlsI GCNGC 2 cut(s) 217, 634
Bme1390I CCNGG 2 cut(s) 96, 582
Bme18I GGWCC 1 cut(s) 470
BmeT110I CYCGRG 1 cut(s) 138
BmgT120I GGNCC 1 cut(s) 470
BmrFI CCNGG 2 cut(s) 96, 582
Bpu14I TTCGAA 1 cut(s) 80
BpuMI CCSGG 1 cut(s) 96
Bsa29I ATCGAT 1 cut(s) 682
BsaHI GRCGYC 1 cut(s) 297
BsaJI CCNNGG 1 cut(s) 486
Bsc4I CCNNNNNNNGG 3 cut(s) 82, 272, 581
Bse1I ACTGG 1 cut(s) 569
BseBI CCWGG 1 cut(s) 582
BseCI ATCGAT 1 cut(s) 682
BseDI CCNNGG 1 cut(s) 486
BseLI CCNNNNNNNGG 3 cut(s) 82, 272, 581
BseNI ACTGG 1 cut(s) 569
BseRI GAGGAG 2 cut(s) 38, 41
BseSI GKGCMC 2 cut(s) 120, 664
BseX3I CGGCCG 1 cut(s) 105
BseXI GCAGC 2 cut(s) 227, 644
Bsh1285I CGRYCG 2 cut(s) 95, 108
BshFI GGCC 1 cut(s) 107
BshVI ATCGAT 1 cut(s) 682
BsiEI CGRYCG 2 cut(s) 95, 108
BsiHKAI GWGCWC 1 cut(s) 120
BsiHKCI CYCGRG 1 cut(s) 138
BsiSI CCGG 4 cut(s) 95, 104, 108, 354
BslFI GGGAC 3 cut(s) 99, 505, 557
BslI CCNNNNNNNGG 3 cut(s) 82, 272, 581
BsmAI GTCTC 2 cut(s) 270, 441
BsmFI GGGAC 3 cut(s) 99, 505, 557
BsnI GGCC 1 cut(s) 107
BsoBI CYCGRG 1 cut(s) 138
Bsp119I TTCGAA 1 cut(s) 80
Bsp1286I GDGCHC 3 cut(s) 120, 145, 664
Bsp143I GATC 1 cut(s) 679
Bsp19I CCATGG 1 cut(s) 486
BspANI GGCC 1 cut(s) 107
BspDI ATCGAT 1 cut(s) 682
BspHI TCATGA 1 cut(s) 165
BspT104I TTCGAA 1 cut(s) 80
BsrI ACTGG 1 cut(s) 569
BssECI CCNNGG 1 cut(s) 486
BssMI GATC 1 cut(s) 679
BssNI GRCGYC 1 cut(s) 297
BssSI CACGAG 1 cut(s) 326
BssT1I CCWWGG 1 cut(s) 486
Bst2BI CACGAG 1 cut(s) 326
Bst2UI CCWGG 1 cut(s) 582
Bst4CI ACNGT 1 cut(s) 72
BstACI GRCGYC 1 cut(s) 297
BstAPI GCANNNNNTGC 1 cut(s) 512
BstBI TTCGAA 1 cut(s) 80
BstC8I GCNNGC 1 cut(s) 600
BstDSI CCRYGG 1 cut(s) 486
BstENI CCTNNNNNAGG 1 cut(s) 270
BstKTI GATC 1 cut(s) 682
BstMAI GTCTC 2 cut(s) 270, 441
BstMBI GATC 1 cut(s) 679
BstMCI CGRYCG 2 cut(s) 95, 108
BstMWI GCNNNNNNNGC 2 cut(s) 512, 629
BstNI CCWGG 1 cut(s) 582
BstNSI RCATGY 2 cut(s) 254, 353
BstSCI CCNGG 2 cut(s) 94, 580
BstSLI GKGCMC 2 cut(s) 120, 664
BstV1I GCAGC 2 cut(s) 227, 644
BstZI CGGCCG 1 cut(s) 105
Bsu15I ATCGAT 1 cut(s) 682
BsuRI GGCC 1 cut(s) 107
BsuTUI ATCGAT 1 cut(s) 682
BtgI CCRYGG 1 cut(s) 486
BtsI GCAGTG 1 cut(s) 609
BtsIMutI CAGTG 2 cut(s) 77, 609
Cac8I GCNNGC 1 cut(s) 600
CciI TCATGA 1 cut(s) 165
Cfr13I GGNCC 1 cut(s) 470
ClaI ATCGAT 1 cut(s) 682
CseI GACGC 1 cut(s) 305
Csp6I GTAC 1 cut(s) 347
CviAII CATG 5 cut(s) 133, 166, 251, 350, 487
CviJI RGCY 7 cut(s) 107, 143, 192, 229, 455, 623, 635
CviKI_1 RGCY 7 cut(s) 107, 143, 192, 229, 455, 623, 635
CviQI GTAC 1 cut(s) 347
DpnI GATC 1 cut(s) 681
DpnII GATC 1 cut(s) 679
EaeI YGGCCR 1 cut(s) 105
EagI CGGCCG 1 cut(s) 105
EclXI CGGCCG 1 cut(s) 105
Eco130I CCWWGG 1 cut(s) 486
Eco24I GRGCYC 1 cut(s) 145
Eco47I GGWCC 1 cut(s) 470
Eco52I CGGCCG 1 cut(s) 105
Eco88I CYCGRG 1 cut(s) 138
EcoNI CCTNNNNNAGG 1 cut(s) 270
EcoRII CCWGG 1 cut(s) 580
EcoT14I CCWWGG 1 cut(s) 486
EcoT38I GRGCYC 1 cut(s) 145
ErhI CCWWGG 1 cut(s) 486
FaeI CATG 5 cut(s) 136, 169, 254, 353, 490
FaqI GGGAC 3 cut(s) 99, 505, 557
FatI CATG 5 cut(s) 132, 165, 250, 349, 486
Fnu4HI GCNGC 2 cut(s) 216, 633
FriOI GRGCYC 1 cut(s) 145
Fsp4HI GCNGC 2 cut(s) 216, 633
GluI GCNGC 2 cut(s) 216, 633
HaeIII GGCC 1 cut(s) 107
HapII CCGG 4 cut(s) 95, 104, 108, 354
HgaI GACGC 1 cut(s) 305
Hin1I GRCGYC 1 cut(s) 297
Hin1II CATG 5 cut(s) 136, 169, 254, 353, 490
HincII GTYRAC 1 cut(s) 6
HindII GTYRAC 1 cut(s) 6
HinfI GANTC 4 cut(s) 87, 136, 262, 305
HpaII CCGG 4 cut(s) 95, 104, 108, 354
Hpy166II GTNNAC 3 cut(s) 6, 118, 256
Hpy188I TCNGA 2 cut(s) 92, 154
Hpy188III TCNNGA 2 cut(s) 166, 328
Hpy8I GTNNAC 3 cut(s) 6, 118, 256
HpyAV CCTTC 6 cut(s) 76, 246, 325, 364, 404, 685
HpyCH4III ACNGT 1 cut(s) 72
HpyCH4IV ACGT 1 cut(s) 114
HpyCH4V TGCA 4 cut(s) 118, 250, 272, 506
HpyF10VI GCNNNNNNNGC 2 cut(s) 512, 629
HpySE526I ACGT 1 cut(s) 114
Hsp92I GRCGYC 1 cut(s) 297
Hsp92II CATG 5 cut(s) 136, 169, 254, 353, 490
Kzo9I GATC 1 cut(s) 679
LmnI GCTCC 2 cut(s) 413, 584
Lsp1109I GCAGC 2 cut(s) 227, 644
MaeII ACGT 1 cut(s) 114
MalI GATC 1 cut(s) 681
MboI GATC 1 cut(s) 679
MboII GAAGA 3 cut(s) 32, 35, 139
MfeI CAATTG 1 cut(s) 588
MhlI GDGCHC 3 cut(s) 120, 145, 664
MluCI AATT 5 cut(s) 146, 161, 499, 520, 588
MlyI GAGTC 2 cut(s) 81, 130
MmeI TCCRAC 3 cut(s) 40, 115, 166
MnlI CCTC 4 cut(s) 59, 62, 203, 393
MseI TTAA 4 cut(s) 122, 177, 498, 555
MslI CAYNNNNRTG 1 cut(s) 70
MspA1I CMGCKG 1 cut(s) 623
MspI CCGG 4 cut(s) 95, 104, 108, 354
MspR9I CCNGG 2 cut(s) 96, 582
MunI CAATTG 1 cut(s) 588
MvaI CCWGG 1 cut(s) 582
MwoI GCNNNNNNNGC 2 cut(s) 512, 629
NciI CCSGG 1 cut(s) 96
NcoI CCATGG 1 cut(s) 486
NdeII GATC 1 cut(s) 679
NlaIII CATG 5 cut(s) 136, 169, 254, 353, 490
NspI RCATGY 2 cut(s) 254, 353
NspV TTCGAA 1 cut(s) 80
PaeR7I CTCGAG 1 cut(s) 138
PagI TCATGA 1 cut(s) 165
PfeI GAWTC 2 cut(s) 262, 305
PkrI GCNGC 2 cut(s) 217, 634
PleI GAGTC 2 cut(s) 81, 130
PpsI GAGTC 2 cut(s) 81, 130
PsiI TTATAA 1 cut(s) 401
Psp6I CCWGG 1 cut(s) 580
PspGI CCWGG 1 cut(s) 580
PspPI GGNCC 1 cut(s) 470
PspXI VCTCGAGB 1 cut(s) 138
PvuII CAGCTG 1 cut(s) 623
RsaI GTAC 1 cut(s) 348
RsaNI GTAC 1 cut(s) 347
RseI CAYNNNNRTG 1 cut(s) 70
SaqAI TTAA 4 cut(s) 122, 177, 498, 555
SatI GCNGC 2 cut(s) 216, 633
Sau3AI GATC 1 cut(s) 679
Sau96I GGNCC 1 cut(s) 470
SchI GAGTC 2 cut(s) 81, 130
ScrFI CCNGG 2 cut(s) 96, 582
SduI GDGCHC 3 cut(s) 120, 145, 664
SetI ASST 7 cut(s) 117, 231, 375, 385, 457, 625, 637
Sfr274I CTCGAG 1 cut(s) 138
SfuI TTCGAA 1 cut(s) 80
SinI GGWCC 1 cut(s) 470
SlaI CTCGAG 1 cut(s) 138
SmiMI CAYNNNNRTG 1 cut(s) 70
SmlI CTYRAG 1 cut(s) 138
SmoI CTYRAG 1 cut(s) 138
Sse9I AATT 5 cut(s) 146, 161, 499, 520, 588
StyD4I CCNGG 2 cut(s) 94, 580
StyI CCWWGG 1 cut(s) 486
TaaI ACNGT 1 cut(s) 72
TaiI ACGT 1 cut(s) 117
TaqI TCGA 3 cut(s) 80, 139, 682
TasI AATT 5 cut(s) 146, 161, 499, 520, 588
TatI WGTACW 1 cut(s) 346
TfiI GAWTC 2 cut(s) 262, 305
Tru1I TTAA 4 cut(s) 122, 177, 498, 555
Tru9I TTAA 4 cut(s) 122, 177, 498, 555
TscAI CASTG 2 cut(s) 77, 609
TseI GCWGC 2 cut(s) 215, 632
TspDTI ATGAA 3 cut(s) 154, 182, 307
TspGWI ACGGA 1 cut(s) 380
TspRI CASTG 2 cut(s) 77, 609
VneI GTGCAC 1 cut(s) 116
VpaK11BI GGWCC 1 cut(s) 470
XagI CCTNNNNNAGG 1 cut(s) 270
XceI RCATGY 2 cut(s) 254, 353
XhoI CTCGAG 1 cut(s) 138
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.