pycom02g05420

leucine-rich repeat extensin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0012081
Physical Location & Seq
Reverse (-)
3618566 .. 3619516
951 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g05420.1

Sequence Viewer

Length: 951 bp
ATGGGTCGGCCCGAATGTGTGTTCTTACACAGGGGTTTTCTGCTCGCCGGATTCTCAAGAAGTTGCAGGCATAGATCGATCAACCACGCCAATCTCAAGGGCAATCTCGTCCAAGAGCTCTCTTTTCTCTCTCAACTCTCTCTCCTCCACCTCAACAGCAATAGATTCTCCGGCCAAATCCCTGAAAGTCTCCGAGACCTCACCGCCCTCCAAGAACTCGACCTCAGCAACAACTTCTTCTCCGGCAGCTTCCCAACGGTCACATTACAAATTCCGAACCTCATCTACCTCGACCTCCGATTCAACAGCTTCTCCGGCACGATCCCGGACGAGCTCTTCAACCGGAAACTCGACGCCATCTTCCTCAACAACAACAATTTCGAAGGCCAAATCCCCGAAAGTTTAGGAAACTCTCAGGCCTCCGTCATCAATTTCGCAAACAACCAGTTCACCGGCAACCTCCCGGCCAGCTTCGGCTTCATGGGGACGACGCTGAAAGAGGTTTTGTTCCTTAACAACCGGTTAACCGGCTGCATTCCCGAAGGAGTCGGGATCTTCACAGACATTAAGGTTTTCGACGCGAGCCATAATTCCCTGATGGGTCATCTCCCCGACACGATTTCTTGCCTGGAAGACATCGAAGTTCTGAACTTGGCGCACAACAATCTGTCTGGGGTGCTGCCGGACTTGGTGTGTTCGTTGAAAAGTCTGGTGAATCTGACCGTTGCTTACAATTTCTTTTCCGGGTTCAGTCAGGAATGTACGAAGTTATTCTACCGGAATGTGGGGTTTGATTTCTCCCTGAATTGCATTCCCGGGTGGAACATGCAGCGGCCGGAGCCGGAATGCTCTCTGATTCCGGGCGGTGGGTTGAACTGCCTGAGAATTCCGGGGTCCAAGCCTCTGGTATGTGGGTCCCTCTACACATCGTCTTCTACTTCTTCTCCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8

Protein Analysis

317

Amino Acids

34.79

Weight (kDa)

5.79

Isoelectric Point (pI)

38.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_8 PF13855 45 - 103 7.2e-06 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012081)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G19780 AT4G29240 AT4G29240
fragaria_vesca FvH4_1g06510
malus_domestica MD15G1200000.v1.1
prunus_persica Prupe.7G217200_v2.0.a1
pyrus_communis pycom02g05420 pycom15g17750
rosa_chinensis RchiOBHm_Chr2g0092261
rosa_laevigata RLG00000016291
rosa_multiflora Rmu_sc0000524.1_g000033 Rmu_sc0030540.1_g000002
rosa_roxburghii Rroxscaffold_2G00149320
rosa_rugosa Rorug02G0024100
rosa_samantha Rh2AG070000 Rh2BG069500 Rh2CG070700 Rh2DG068500
rosa_wichuraiana Rw2G006220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 581
AciI CCGC 3 cut(s) 204, 832, 864
AclWI GGATC 2 cut(s) 316, 560
AcoI YGGCCR 3 cut(s) 172, 465, 833
AcsI RAATTY 2 cut(s) 270, 885
AcyI GRCGYC 1 cut(s) 354
AfaI GTAC 1 cut(s) 763
AfiI CCNNNNNNNGG 2 cut(s) 784, 866
AgeI ACCGGT 1 cut(s) 519
AgsI TTSAA 4 cut(s) 304, 340, 703, 874
AjnI CCWGG 1 cut(s) 627
AluBI AGCT 5 cut(s) 118, 249, 309, 334, 471
AluI AGCT 5 cut(s) 118, 249, 309, 334, 471
Alw21I GWGCWC 2 cut(s) 120, 336
Alw26I GTCTC 2 cut(s) 189, 194
AlwI GGATC 2 cut(s) 316, 560
Ama87I CYCGRG 1 cut(s) 815
AoxI GGCC 6 cut(s) 8, 172, 385, 417, 465, 833
ApeKI GCWGC 4 cut(s) 246, 531, 679, 829
ApoI RAATTY 2 cut(s) 270, 885
AsiGI ACCGGT 1 cut(s) 519
Asp700I GAANNNNTTC 1 cut(s) 770
AspLEI GCGC 1 cut(s) 658
AspS9I GGNCC 3 cut(s) 9, 894, 915
AsuC2I CCSGG 7 cut(s) 326, 464, 745, 816, 817, 861, 891
AsuHPI GGTGA 3 cut(s) 193, 442, 724
AsuII TTCGAA 1 cut(s) 381
AvaI CYCGRG 1 cut(s) 815
AvaII GGWCC 2 cut(s) 894, 915
BanII GRGCYC 2 cut(s) 120, 336
BarI GAAGNNNNNNTAC 2 cut(s) 758, 790
BbsI GAAGAC 2 cut(s) 639, 924
Bbv12I GWGCWC 2 cut(s) 120, 336
BbvCI CCTCAGC 1 cut(s) 224
BbvI GCAGC 4 cut(s) 258, 518, 666, 841
BccI CCATC 2 cut(s) 365, 592
BciT130I CCWGG 1 cut(s) 629
BcnI CCSGG 7 cut(s) 326, 464, 745, 816, 817, 861, 891
BcoDI GTCTC 2 cut(s) 189, 194
BisI GCNGC 5 cut(s) 247, 532, 680, 830, 833
BlsI GCNGC 5 cut(s) 248, 533, 681, 831, 834
Bme1390I CCNGG 8 cut(s) 326, 464, 629, 745, 816, 817, 861, 891
Bme18I GGWCC 2 cut(s) 894, 915
BmeT110I CYCGRG 1 cut(s) 815
BmgT120I GGNCC 3 cut(s) 9, 894, 915
BmiI GGNNCC 4 cut(s) 840, 895, 916, 917
BmrFI CCNGG 8 cut(s) 326, 464, 629, 745, 816, 817, 861, 891
BpiI GAAGAC 2 cut(s) 639, 924
Bpu10I CCTNAGC 1 cut(s) 224
Bpu14I TTCGAA 1 cut(s) 381
BpuEI CTTGAG 2 cut(s) 40, 80
BpuMI CCSGG 7 cut(s) 326, 464, 745, 816, 817, 861, 891
Bsa29I ATCGAT 1 cut(s) 77
BsaHI GRCGYC 1 cut(s) 354
BsaI GGTCTC 1 cut(s) 189
BsaJI CCNNGG 2 cut(s) 815, 890
BsaWI WCCGGW 3 cut(s) 342, 519, 777
BsaXI ACNNNNNCTCC 7 cut(s) 126, 156, 224, 254, 296, 326, 928
Bsc4I CCNNNNNNNGG 2 cut(s) 784, 866
Bse118I RCCGGY 3 cut(s) 452, 519, 527
Bse1I ACTGG 1 cut(s) 445
BseBI CCWGG 1 cut(s) 629
BseCI ATCGAT 1 cut(s) 77
BseDI CCNNGG 2 cut(s) 815, 890
BseLI CCNNNNNNNGG 2 cut(s) 784, 866
BseMII CTCAG 3 cut(s) 238, 428, 872
BseNI ACTGG 1 cut(s) 445
BseRI GAGGAG 1 cut(s) 134
BseX3I CGGCCG 1 cut(s) 833
BseXI GCAGC 4 cut(s) 258, 518, 666, 841
Bsh1236I CGCG 1 cut(s) 581
Bsh1285I CGRYCG 1 cut(s) 836
BshFI GGCC 6 cut(s) 10, 174, 387, 419, 467, 835
BshTI ACCGGT 1 cut(s) 519
BshVI ATCGAT 1 cut(s) 77
BsiEI CGRYCG 1 cut(s) 836
BsiHKAI GWGCWC 2 cut(s) 120, 336
BsiHKCI CYCGRG 1 cut(s) 815
BslFI GGGAC 2 cut(s) 499, 901
BslI CCNNNNNNNGG 2 cut(s) 784, 866
BsmAI GTCTC 2 cut(s) 189, 194
BsmFI GGGAC 2 cut(s) 499, 901
BsmI GAATGC 3 cut(s) 534, 810, 851
BsnI GGCC 6 cut(s) 10, 174, 387, 419, 467, 835
Bso31I GGTCTC 1 cut(s) 189
BsoBI CYCGRG 1 cut(s) 815
Bsp119I TTCGAA 1 cut(s) 381
Bsp1286I GDGCHC 2 cut(s) 120, 336
Bsp143I GATC 4 cut(s) 74, 78, 321, 552
BspACI CCGC 3 cut(s) 204, 832, 864
BspANI GGCC 6 cut(s) 10, 174, 387, 419, 467, 835
BspCNI CTCAG 3 cut(s) 237, 427, 873
BspDI ATCGAT 1 cut(s) 77
BspFNI CGCG 1 cut(s) 581
BspLI GGNNCC 4 cut(s) 840, 895, 916, 917
BspPI GGATC 2 cut(s) 316, 560
BspQI GCTCTTC 1 cut(s) 341
BspT104I TTCGAA 1 cut(s) 381
BspTNI GGTCTC 1 cut(s) 189
BsrFI RCCGGY 3 cut(s) 452, 519, 527
BsrI ACTGG 1 cut(s) 445
BssAI RCCGGY 3 cut(s) 452, 519, 527
BssECI CCNNGG 2 cut(s) 815, 890
BssMI GATC 4 cut(s) 74, 78, 321, 552
BssNI GRCGYC 1 cut(s) 354
Bst2UI CCWGG 1 cut(s) 629
Bst4CI ACNGT 2 cut(s) 259, 724
Bst6I CTCTTC 1 cut(s) 341
BstACI GRCGYC 1 cut(s) 354
BstBI TTCGAA 1 cut(s) 381
BstC8I GCNNGC 4 cut(s) 45, 68, 469, 583
BstDEI CTNAG 3 cut(s) 224, 414, 881
BstFNI CGCG 1 cut(s) 581
BstHHI GCGC 1 cut(s) 658
BstKTI GATC 4 cut(s) 77, 81, 324, 555
BstMAI GTCTC 2 cut(s) 189, 194
BstMBI GATC 4 cut(s) 74, 78, 321, 552
BstMCI CGRYCG 1 cut(s) 836
BstMWI GCNNNNNNNGC 2 cut(s) 315, 838
BstNI CCWGG 1 cut(s) 629
BstNSI RCATGY 1 cut(s) 829
BstSCI CCNGG 8 cut(s) 324, 462, 627, 743, 814, 815, 859, 889
BstUI CGCG 1 cut(s) 581
BstV1I GCAGC 4 cut(s) 258, 518, 666, 841
BstV2I GAAGAC 2 cut(s) 639, 924
BstX2I RGATCY 1 cut(s) 552
BstXI CCANNNNNNTGG 1 cut(s) 904
BstYI RGATCY 1 cut(s) 552
BstZI CGGCCG 1 cut(s) 833
Bsu15I ATCGAT 1 cut(s) 77
BsuRI GGCC 6 cut(s) 10, 174, 387, 419, 467, 835
BsuTUI ATCGAT 1 cut(s) 77
Cac8I GCNNGC 4 cut(s) 45, 68, 469, 583
CfoI GCGC 1 cut(s) 658
Cfr10I RCCGGY 3 cut(s) 452, 519, 527
Cfr13I GGNCC 3 cut(s) 9, 894, 915
Cfr9I CCCGGG 1 cut(s) 815
ClaI ATCGAT 1 cut(s) 77
CseI GACGC 3 cut(s) 362, 499, 587
Csp6I GTAC 1 cut(s) 762
CspAI ACCGGT 1 cut(s) 519
CviAII CATG 2 cut(s) 481, 826
CviQI GTAC 1 cut(s) 762
DdeI CTNAG 3 cut(s) 224, 414, 881
DpnI GATC 4 cut(s) 76, 80, 323, 554
DpnII GATC 4 cut(s) 74, 78, 321, 552
EaeI YGGCCR 3 cut(s) 172, 465, 833
EagI CGGCCG 1 cut(s) 833
Eam1104I CTCTTC 1 cut(s) 341
EarI CTCTTC 1 cut(s) 341
Ecl136II GAGCTC 2 cut(s) 118, 334
EclXI CGGCCG 1 cut(s) 833
Eco147I AGGCCT 1 cut(s) 419
Eco24I GRGCYC 2 cut(s) 120, 336
Eco31I GGTCTC 1 cut(s) 189
Eco47I GGWCC 2 cut(s) 894, 915
Eco52I CGGCCG 1 cut(s) 833
Eco53kI GAGCTC 2 cut(s) 118, 334
Eco88I CYCGRG 1 cut(s) 815
EcoICRI GAGCTC 2 cut(s) 118, 334
EcoO109I RGGNCCY 1 cut(s) 915
EcoRI GAATTC 1 cut(s) 885
EcoRII CCWGG 1 cut(s) 627
EcoT38I GRGCYC 2 cut(s) 120, 336
FaeI CATG 2 cut(s) 484, 829
FaiI YATR 6 cut(s) 72, 482, 588, 827, 910, 949
FaqI GGGAC 2 cut(s) 499, 901
FatI CATG 2 cut(s) 480, 825
Fnu4HI GCNGC 5 cut(s) 247, 532, 680, 830, 833
FriOI GRGCYC 2 cut(s) 120, 336
Fsp4HI GCNGC 5 cut(s) 247, 532, 680, 830, 833
GlaI GCGC 1 cut(s) 657
GluI GCNGC 5 cut(s) 247, 532, 680, 830, 833
HaeIII GGCC 6 cut(s) 10, 174, 387, 419, 467, 835
HgaI GACGC 3 cut(s) 362, 499, 587
HhaI GCGC 1 cut(s) 658
Hin1I GRCGYC 1 cut(s) 354
Hin1II CATG 2 cut(s) 484, 829
Hin6I GCGC 1 cut(s) 656
HinP1I GCGC 1 cut(s) 656
HincII GTYRAC 1 cut(s) 525
HindII GTYRAC 1 cut(s) 525
HinfI GANTC 6 cut(s) 51, 165, 300, 546, 715, 856
HpaI GTTAAC 1 cut(s) 525
HphI GGTGA 3 cut(s) 193, 442, 724
Hpy166II GTNNAC 2 cut(s) 450, 525
Hpy188I TCNGA 6 cut(s) 194, 276, 299, 648, 720, 855
Hpy188III TCNNGA 4 cut(s) 57, 539, 550, 755
Hpy8I GTNNAC 2 cut(s) 450, 525
Hpy99I CGWCG 3 cut(s) 356, 493, 581
HpyAV CCTTC 2 cut(s) 377, 536
HpyCH4III ACNGT 2 cut(s) 259, 724
HpyCH4V TGCA 4 cut(s) 66, 534, 810, 829
HpyF10VI GCNNNNNNNGC 2 cut(s) 315, 838
HpyF3I CTNAG 3 cut(s) 224, 414, 881
Hsp92I GRCGYC 1 cut(s) 354
Hsp92II CATG 2 cut(s) 484, 829
HspAI GCGC 1 cut(s) 656
KflI GGGWCCC 1 cut(s) 915
KspAI GTTAAC 1 cut(s) 525
Kzo9I GATC 4 cut(s) 74, 78, 321, 552
LguI GCTCTTC 1 cut(s) 341
LmnI GCTCC 1 cut(s) 838
Lsp1109I GCAGC 4 cut(s) 258, 518, 666, 841
MaeIII GTNAC 1 cut(s) 259
MalI GATC 4 cut(s) 76, 80, 323, 554
MboI GATC 4 cut(s) 74, 78, 321, 552
MboII GAAGA 7 cut(s) 229, 328, 352, 547, 644, 924, 933
MflI RGATCY 1 cut(s) 552
MhlI GDGCHC 2 cut(s) 120, 336
MluCI AATT 7 cut(s) 270, 376, 430, 589, 733, 805, 885
MlyI GAGTC 1 cut(s) 555
MroXI GAANNNNTTC 1 cut(s) 770
MseI TTAA 3 cut(s) 513, 524, 567
MspA1I CMGCKG 1 cut(s) 832
MspR9I CCNGG 8 cut(s) 326, 464, 629, 745, 816, 817, 861, 891
Mva1269I GAATGC 3 cut(s) 534, 810, 851
MvaI CCWGG 1 cut(s) 629
MvnI CGCG 1 cut(s) 581
MwoI GCNNNNNNNGC 2 cut(s) 315, 838
NciI CCSGG 7 cut(s) 326, 464, 745, 816, 817, 861, 891
NdeII GATC 4 cut(s) 74, 78, 321, 552
NlaIII CATG 2 cut(s) 484, 829
NlaIV GGNNCC 4 cut(s) 840, 895, 916, 917
NmuCI GTSAC 1 cut(s) 259
NspI RCATGY 1 cut(s) 829
NspV TTCGAA 1 cut(s) 381
PceI AGGCCT 1 cut(s) 419
PciSI GCTCTTC 1 cut(s) 341
PctI GAATGC 3 cut(s) 534, 810, 851
PdmI GAANNNNTTC 1 cut(s) 770
PfeI GAWTC 5 cut(s) 51, 165, 300, 715, 856
PfoI TCCNGGA 1 cut(s) 324
PinAI ACCGGT 1 cut(s) 519
PkrI GCNGC 5 cut(s) 248, 533, 681, 831, 834
PleI GAGTC 1 cut(s) 554
PpsI GAGTC 1 cut(s) 554
PpuMI RGGWCCY 1 cut(s) 915
Psp124BI GAGCTC 2 cut(s) 120, 336
Psp5II RGGWCCY 1 cut(s) 915
Psp6I CCWGG 1 cut(s) 627
PspGI CCWGG 1 cut(s) 627
PspN4I GGNNCC 4 cut(s) 840, 895, 916, 917
PspPI GGNCC 3 cut(s) 9, 894, 915
PspPPI RGGWCCY 1 cut(s) 915
PsrI GAACNNNNNNTAC 2 cut(s) 269, 301
PsuI RGATCY 1 cut(s) 552
RsaI GTAC 1 cut(s) 763
RsaNI GTAC 1 cut(s) 762
SacI GAGCTC 2 cut(s) 120, 336
SapI GCTCTTC 1 cut(s) 341
SaqAI TTAA 3 cut(s) 513, 524, 567
SatI GCNGC 5 cut(s) 247, 532, 680, 830, 833
Sau3AI GATC 4 cut(s) 74, 78, 321, 552
Sau96I GGNCC 3 cut(s) 9, 894, 915
SchI GAGTC 1 cut(s) 555
ScrFI CCNGG 8 cut(s) 326, 464, 629, 745, 816, 817, 861, 891
SduI GDGCHC 2 cut(s) 120, 336
SfuI TTCGAA 1 cut(s) 381
SinI GGWCC 2 cut(s) 894, 915
SmaI CCCGGG 1 cut(s) 817
SmlI CTYRAG 2 cut(s) 55, 95
SmoI CTYRAG 2 cut(s) 55, 95
Sse9I AATT 7 cut(s) 270, 376, 430, 589, 733, 805, 885
SseBI AGGCCT 1 cut(s) 419
SsiI CCGC 3 cut(s) 204, 832, 864
SstI GAGCTC 2 cut(s) 120, 336
StuI AGGCCT 1 cut(s) 419
StyD4I CCNGG 8 cut(s) 324, 462, 627, 743, 814, 815, 859, 889
TaaI ACNGT 2 cut(s) 259, 724
TaqI TCGA 7 cut(s) 77, 219, 291, 351, 381, 576, 639
TasI AATT 7 cut(s) 270, 376, 430, 589, 733, 805, 885
TauI GCSGC 1 cut(s) 835
TfiI GAWTC 5 cut(s) 51, 165, 300, 715, 856
Tru1I TTAA 3 cut(s) 513, 524, 567
Tru9I TTAA 3 cut(s) 513, 524, 567
TseFI GTSAC 1 cut(s) 259
TseI GCWGC 4 cut(s) 246, 531, 679, 829
Tsp45I GTSAC 1 cut(s) 259
TspDTI ATGAA 1 cut(s) 469
TspGWI ACGGA 1 cut(s) 412
TspMI CCCGGG 1 cut(s) 815
VpaK11BI GGWCC 2 cut(s) 894, 915
XapI RAATTY 2 cut(s) 270, 885
XceI RCATGY 1 cut(s) 829
XmaI CCCGGG 1 cut(s) 815
XmnI GAANNNNTTC 1 cut(s) 770
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.