pycom02g06820

Hydroxyproline-rich glycoprotein family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0012501
Physical Location & Seq
Forward (+)
4726794 .. 4728524
1731 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1626 bp
ATGCCTCCCCGCCGCTCACCATCGCCACTTCTAAGTTCACCGTTGCTCATCATCCTGTTCCCCATTATCATCTCCATCATCCTCTTCTACGCCATCCCTCAATTCCTCTCCCTCACCTCTCAACTCCTCCGCCCCACCATTTCCGTCAAGAAAAGTTGGGACTCCCTCAACGTGCTTCTCGTCATTTTCGCAATCCTATGCGGCGTTTTCGCCAAGCTAAATGACGACGCATCATCCTCTGAAGAAGATCGCATTCCCGACACTAATATTCCGCACCCTTTGGACAATATTCGTACAAACAGAGCCAACATATCGGAATCGGAATCAGCTGCATCCCTACTTCCGCAACAATGGTTTGGATATTCAGATAGGAGGACGCCTGAATCCGGCGGCGACCGGTTGAGGAGGAGCAGCAGTTCCTACCCGGATCTGAGGCCACTCGGGCAGGACTCGGTGTGGGAAACTCGGGATCACAACAAGTCACAGTTTCACTTTTTCGACGACTTCGAAATTGGTGATTACCGTCGTGTCCCGCCAGTTTCACACCAACGGCGACCCAAGGAGTACTCTGATGTAATTAAGGAGATTCCGGTGGATACTTTTGTGCTTCACTCTTCTCCTCCGCTGCCATCCAAGCCTTCGACACCACCACCTCCGCCACCTCCTCCGCCCCGTAATCCGAAACCGAGAAGGACGTATCAGACTGTTGGACGGAGAGATCAAAAGACGGAGAAAGTAGTTAATAAAAATGTTATTAATGAGGCTGAAGAGTTGGAGAAAGTTCGAACTCCGCCACCGACGCCGCCGCATCCACTGCCACGTCCGGTCGTGCCGCCGTCACCAATTCGGATTAGATCAGAGTCGAAGCGTAGAAAGAGCAATGTGAAGAAGGAGATAGCGATGGTTTGGGCTTCGGTTCTGTCTAACCAGAGAAAGAGGAAGAAGCAGCACAAGGTCACCGGTACCAGAAACATTTACGAAACCGCTGCTTCTCTGCATGAACACGAACAACAAGACAGAGAAGGGGAATATTTTTTCACAAGACAGCCACCATCGCCACCTCCTCCACCGCCACCTCCGCCGCCGCCACATTCTGTATTTCACAATCTATTCAAAAAGGGAATGAGCAAAACTAAGAAAGCTCACTCCGTCTTGACGCCTCCGACTCCGCCACCACCGCCACCGCAGCCGGCATTGCGAAAACACAAGAGTTGGAATACTAGTTTCCCGCTGGCACCACCAACTCCGCCGAAACAACCGCCTCCGGCTACTTCCCGGCCGCGACACTCCCCCTCCAGTGGCCGGCCGCCGTTGCCAACAAAGGCGAACAGTTATTTATACGATGGGAAAATACAGAGCGCGCAGAGCTCGCGCTGTGGATCTCCTGAACTGGAGGACGTTGACGACGTTGACGACGTTGATGCATCTCCACGTAAGGAAGAAGACATGAAGTCAAAGTCCGAAGTACAGAGTAAAGTCAACGCGATGGACGGCGGCAGCGGATCCTCCGTGTTCTGCCCTAGCCCAGATGTTAATACGAAAGCTGATAACTTCATTGCTAGGCTGAAAGATGAGTGGAGGCTTGAGAAGATGAACTCAATGAGGCAGAAAATGAAGATGCCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04062 ko04144 ko04520 ko04530 ko04666 ko04810 ko05100 ko05130 ko05131 ko05132 ko05231 map04062 map04144 map04520 map04530 map04666 map04810 map05100 map05130 map05131 map05132 map05231
Pfam Domains
Protein Families
DUF761

Protein Analysis

542

Amino Acids

60.95

Weight (kDa)

9.81

Isoelectric Point (pI)

80.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF761 PF05553 509 - 540 4.8e-06 Cotton fibre expressed protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 962
AccB1I GGYRCC 2 cut(s) 962, 1234
AccBSI CCGCTC 1 cut(s) 15
AccII CGCG 4 cut(s) 1282, 1361, 1372, 1484
AclWI GGATC 5 cut(s) 435, 477, 1387, 1497, 1510
AcoI YGGCCR 3 cut(s) 1277, 1300, 1304
AcuI CTGAAG 2 cut(s) 261, 786
AcyI GRCGYC 3 cut(s) 377, 800, 1157
AfaI GTAC 4 cut(s) 295, 566, 964, 1467
AfiI CCNNNNNNNGG 3 cut(s) 386, 1298, 1302
AgeI ACCGGT 2 cut(s) 396, 959
AgsI TTSAA 1 cut(s) 1114
AhdI GACNNNNNGTC 1 cut(s) 1450
AhlI ACTAGT 1 cut(s) 1220
AjiI CACGTC 1 cut(s) 821
AloI GAACNNNNNNTCC 2 cut(s) 400, 432
AluBI AGCT 5 cut(s) 217, 329, 1142, 1368, 1544
AluI AGCT 5 cut(s) 217, 329, 1142, 1368, 1544
Alw21I GWGCWC 1 cut(s) 1370
AlwI GGATC 5 cut(s) 435, 477, 1387, 1497, 1510
Ama87I CYCGRG 2 cut(s) 440, 465
AoxI GGCC 4 cut(s) 434, 1277, 1300, 1304
ApeKI GCWGC 7 cut(s) 329, 411, 625, 946, 986, 1186, 1497
AseI ATTAAT 1 cut(s) 756
AsiGI ACCGGT 2 cut(s) 396, 959
Asp718I GGTACC 1 cut(s) 962
AspLEI GCGC 3 cut(s) 1361, 1363, 1374
AsuC2I CCSGG 2 cut(s) 425, 1276
AsuHPI GGTGA 6 cut(s) 9, 30, 106, 527, 831, 949
AsuII TTCGAA 2 cut(s) 507, 784
AvaI CYCGRG 2 cut(s) 440, 465
BaeI ACNNNNGTAYC 2 cut(s) 588, 621
BamHI GGATCC 1 cut(s) 1502
BanI GGYRCC 2 cut(s) 962, 1234
BanII GRGCYC 1 cut(s) 1370
BbsI GAAGAC 1 cut(s) 1449
Bbv12I GWGCWC 1 cut(s) 1370
BbvI GCAGC 7 cut(s) 316, 423, 612, 958, 973, 1198, 1509
BccI CCATC 8 cut(s) 28, 83, 101, 637, 895, 1060, 1337, 1480
BceAI ACGGC 4 cut(s) 566, 820, 1294, 1507
BcgI CGANNNNNNTGC 4 cut(s) 968, 1002, 1403, 1437
BciVI GTATCC 1 cut(s) 589
BcnI CCSGG 2 cut(s) 425, 1276
BcuI ACTAGT 1 cut(s) 1220
BfaI CTAG 3 cut(s) 1221, 1521, 1560
BfuI GTATCC 1 cut(s) 589
BglI GCCNNNNNGGC 1 cut(s) 442
BmcAI AGTACT 1 cut(s) 566
Bme1390I CCNGG 2 cut(s) 425, 1276
BmeRI GACNNNNNGTC 1 cut(s) 1450
BmeT110I CYCGRG 2 cut(s) 440, 465
BmgBI CACGTC 1 cut(s) 821
BmiI GGNNCC 3 cut(s) 964, 1236, 1504
BmrFI CCNGG 2 cut(s) 425, 1276
BmsI GCATC 6 cut(s) 239, 341, 817, 1411, 1433, 1608
BpiI GAAGAC 1 cut(s) 1449
BpmI CTGGAG 2 cut(s) 1279, 1412
Bpu14I TTCGAA 2 cut(s) 507, 784
BpuEI CTTGAG 1 cut(s) 1604
BpuMI CCSGG 2 cut(s) 425, 1276
BsaAI YACGTR 1 cut(s) 1433
BsaHI GRCGYC 3 cut(s) 377, 800, 1157
BsaJI CCNNGG 1 cut(s) 558
BsaWI WCCGGW 4 cut(s) 396, 589, 823, 959
BsaXI ACNNNNNCTCC 4 cut(s) 400, 430, 1277, 1307
Bsc4I CCNNNNNNNGG 3 cut(s) 386, 1298, 1302
Bse118I RCCGGY 4 cut(s) 396, 959, 1189, 1302
Bse1I ACTGG 3 cut(s) 536, 1296, 1395
Bse3DI GCAATG 3 cut(s) 886, 1193, 1554
BseDI CCNNGG 1 cut(s) 558
BseGI GGATG 7 cut(s) 51, 78, 93, 233, 332, 629, 808
BseLI CCNNNNNNNGG 3 cut(s) 386, 1298, 1302
BseMI GCAATG 3 cut(s) 886, 1193, 1554
BseMII CTCAG 1 cut(s) 422
BseNI ACTGG 3 cut(s) 536, 1296, 1395
BsePI GCGCGC 1 cut(s) 1359
BseRI GAGGAG 6 cut(s) 116, 418, 421, 609, 654, 1053
BseX3I CGGCCG 2 cut(s) 1277, 1304
BseXI GCAGC 7 cut(s) 316, 423, 612, 958, 973, 1198, 1509
Bsh1236I CGCG 4 cut(s) 1282, 1361, 1372, 1484
Bsh1285I CGRYCG 4 cut(s) 397, 828, 1280, 1307
BshFI GGCC 4 cut(s) 436, 1279, 1302, 1306
BshNI GGYRCC 2 cut(s) 962, 1234
BshTI ACCGGT 2 cut(s) 396, 959
BsiEI CGRYCG 4 cut(s) 397, 828, 1280, 1307
BsiHKAI GWGCWC 1 cut(s) 1370
BsiHKCI CYCGRG 2 cut(s) 440, 465
BslFI GGGAC 2 cut(s) 173, 515
BslI CCNNNNNNNGG 3 cut(s) 386, 1298, 1302
BsmFI GGGAC 2 cut(s) 173, 515
BsmI GAATGC 1 cut(s) 252
BsnI GGCC 4 cut(s) 436, 1279, 1302, 1306
BsoBI CYCGRG 2 cut(s) 440, 465
Bsp119I TTCGAA 2 cut(s) 507, 784
Bsp1286I GDGCHC 1 cut(s) 1370
Bsp143I GATC 7 cut(s) 247, 427, 469, 718, 854, 1379, 1502
BspANI GGCC 4 cut(s) 436, 1279, 1302, 1306
BspCNI CTCAG 1 cut(s) 423
BspFNI CGCG 4 cut(s) 1282, 1361, 1372, 1484
BspLI GGNNCC 3 cut(s) 964, 1236, 1504
BspPI GGATC 5 cut(s) 435, 477, 1387, 1497, 1510
BspT104I TTCGAA 2 cut(s) 507, 784
BspT107I GGYRCC 2 cut(s) 962, 1234
BsrBI CCGCTC 1 cut(s) 15
BsrDI GCAATG 3 cut(s) 886, 1193, 1554
BsrFI RCCGGY 4 cut(s) 396, 959, 1189, 1302
BsrI ACTGG 3 cut(s) 536, 1296, 1395
BssAI RCCGGY 4 cut(s) 396, 959, 1189, 1302
BssECI CCNNGG 1 cut(s) 558
BssHII GCGCGC 1 cut(s) 1359
BssMI GATC 7 cut(s) 247, 427, 469, 718, 854, 1379, 1502
BssNI GRCGYC 3 cut(s) 377, 800, 1157
BssT1I CCWWGG 1 cut(s) 558
Bst4CI ACNGT 5 cut(s) 42, 486, 524, 706, 1331
Bst6I CTCTTC 3 cut(s) 89, 619, 762
BstACI GRCGYC 3 cut(s) 377, 800, 1157
BstAPI GCANNNNNTGC 1 cut(s) 814
BstBAI YACGTR 1 cut(s) 1433
BstBI TTCGAA 2 cut(s) 507, 784
BstC8I GCNNGC 5 cut(s) 1191, 1233, 1304, 1361, 1370
BstDEI CTNAG 3 cut(s) 32, 431, 1134
BstEII GGTNACC 1 cut(s) 955
BstF5I GGATG 7 cut(s) 51, 78, 93, 233, 332, 629, 808
BstFNI CGCG 4 cut(s) 1282, 1361, 1372, 1484
BstHHI GCGC 3 cut(s) 1361, 1363, 1374
BstKTI GATC 7 cut(s) 250, 430, 472, 721, 857, 1382, 1505
BstMBI GATC 7 cut(s) 247, 427, 469, 718, 854, 1379, 1502
BstMCI CGRYCG 4 cut(s) 397, 828, 1280, 1307
BstPI GGTNACC 1 cut(s) 955
BstSCI CCNGG 2 cut(s) 423, 1274
BstUI CGCG 4 cut(s) 1282, 1361, 1372, 1484
BstV1I GCAGC 7 cut(s) 316, 423, 612, 958, 973, 1198, 1509
BstV2I GAAGAC 1 cut(s) 1449
BstX2I RGATCY 3 cut(s) 427, 1379, 1502
BstYI RGATCY 3 cut(s) 427, 1379, 1502
BstZI CGGCCG 2 cut(s) 1277, 1304
BsuI GTATCC 1 cut(s) 589
BsuRI GGCC 4 cut(s) 436, 1279, 1302, 1306
BtgZI GCGATG 4 cut(s) 6, 914, 1038, 1499
BtrI CACGTC 1 cut(s) 821
BtsCI GGATG 7 cut(s) 51, 78, 93, 233, 332, 629, 808
BtsI GCAGTG 1 cut(s) 812
BtsIMutI CAGTG 2 cut(s) 812, 1303
Cac8I GCNNGC 5 cut(s) 1191, 1233, 1304, 1361, 1370
CfoI GCGC 3 cut(s) 1361, 1363, 1374
Cfr10I RCCGGY 4 cut(s) 396, 959, 1189, 1302
CseI GACGC 4 cut(s) 236, 385, 808, 1165
Csp6I GTAC 4 cut(s) 294, 565, 963, 1466
CspAI ACCGGT 2 cut(s) 396, 959
CviAII CATG 2 cut(s) 998, 1447
CviQI GTAC 4 cut(s) 294, 565, 963, 1466
DdeI CTNAG 3 cut(s) 32, 431, 1134
DpnI GATC 7 cut(s) 249, 429, 471, 720, 856, 1381, 1504
DpnII GATC 7 cut(s) 247, 427, 469, 718, 854, 1379, 1502
DriI GACNNNNNGTC 1 cut(s) 1450
EaeI YGGCCR 3 cut(s) 1277, 1300, 1304
EagI CGGCCG 2 cut(s) 1277, 1304
Eam1104I CTCTTC 3 cut(s) 89, 619, 762
Eam1105I GACNNNNNGTC 1 cut(s) 1450
EarI CTCTTC 3 cut(s) 89, 619, 762
EciI GGCGGA 7 cut(s) 119, 645, 657, 780, 1068, 1158, 1236
Ecl136II GAGCTC 1 cut(s) 1368
EclXI CGGCCG 2 cut(s) 1277, 1304
Eco130I CCWWGG 1 cut(s) 558
Eco24I GRGCYC 1 cut(s) 1370
Eco52I CGGCCG 2 cut(s) 1277, 1304
Eco53kI GAGCTC 1 cut(s) 1368
Eco57I CTGAAG 2 cut(s) 261, 786
Eco88I CYCGRG 2 cut(s) 440, 465
Eco91I GGTNACC 1 cut(s) 955
EcoICRI GAGCTC 1 cut(s) 1368
EcoO65I GGTNACC 1 cut(s) 955
EcoT14I CCWWGG 1 cut(s) 558
EcoT22I ATGCAT 1 cut(s) 1426
EcoT38I GRGCYC 1 cut(s) 1370
ErhI CCWWGG 1 cut(s) 558
FaeI CATG 2 cut(s) 1001, 1450
FaiI YATR 5 cut(s) 199, 311, 999, 1339, 1448
FaqI GGGAC 2 cut(s) 173, 515
FatI CATG 2 cut(s) 997, 1446
FauI CCCGC 3 cut(s) 17, 540, 1236
FokI GGATG 7 cut(s) 38, 65, 80, 220, 319, 616, 795
FriOI GRGCYC 1 cut(s) 1370
FseI GGCCGGCC 1 cut(s) 1306
FspBI CTAG 3 cut(s) 1221, 1521, 1560
GlaI GCGC 3 cut(s) 1360, 1362, 1373
GsuI CTGGAG 2 cut(s) 1279, 1412
HaeIII GGCC 4 cut(s) 436, 1279, 1302, 1306
HgaI GACGC 4 cut(s) 236, 385, 808, 1165
HhaI GCGC 3 cut(s) 1361, 1363, 1374
Hin1I GRCGYC 3 cut(s) 377, 800, 1157
Hin1II CATG 2 cut(s) 1001, 1450
Hin6I GCGC 3 cut(s) 1359, 1361, 1372
HinP1I GCGC 3 cut(s) 1359, 1361, 1372
HincII GTYRAC 3 cut(s) 1402, 1411, 1480
HindII GTYRAC 3 cut(s) 1402, 1411, 1480
HinfI GANTC 8 cut(s) 161, 317, 323, 383, 449, 586, 860, 1165
HphI GGTGA 6 cut(s) 9, 30, 106, 527, 831, 949
Hpy166II GTNNAC 4 cut(s) 38, 1402, 1411, 1480
Hpy188III TCNNGA 5 cut(s) 148, 257, 467, 1153, 1385
Hpy8I GTNNAC 4 cut(s) 38, 1402, 1411, 1480
Hpy99I CGWCG 6 cut(s) 230, 503, 528, 802, 1409, 1418
HpyAV CCTTC 4 cut(s) 648, 684, 883, 1016
HpyCH4III ACNGT 5 cut(s) 42, 486, 524, 706, 1331
HpyCH4IV ACGT 7 cut(s) 171, 695, 820, 1398, 1407, 1416, 1432
HpyCH4V TGCA 3 cut(s) 332, 997, 1424
HpyF3I CTNAG 3 cut(s) 32, 431, 1134
HpySE526I ACGT 7 cut(s) 171, 695, 820, 1398, 1407, 1416, 1432
Hsp92I GRCGYC 3 cut(s) 377, 800, 1157
Hsp92II CATG 2 cut(s) 1001, 1450
HspAI GCGC 3 cut(s) 1359, 1361, 1372
KpnI GGTACC 1 cut(s) 966
KroI GCCGGC 2 cut(s) 1189, 1302
KroNI GCCGGC 2 cut(s) 1191, 1304
Kzo9I GATC 7 cut(s) 247, 427, 469, 718, 854, 1379, 1502
LmnI GCTCC 1 cut(s) 408
Lsp1109I GCAGC 7 cut(s) 316, 423, 612, 958, 973, 1198, 1509
LweI GCATC 6 cut(s) 239, 341, 817, 1411, 1433, 1608
MaeI CTAG 3 cut(s) 1221, 1521, 1560
MaeII ACGT 7 cut(s) 171, 695, 820, 1398, 1407, 1416, 1432
MaeIII GTNAC 3 cut(s) 480, 837, 955
MalI GATC 7 cut(s) 249, 429, 471, 720, 856, 1381, 1504
MbiI CCGCTC 1 cut(s) 15
MboI GATC 7 cut(s) 247, 427, 469, 718, 854, 1379, 1502
MflI RGATCY 3 cut(s) 427, 1379, 1502
MhlI GDGCHC 1 cut(s) 1370
MluCI AATT 4 cut(s) 101, 510, 576, 843
MlyI GAGTC 4 cut(s) 155, 443, 869, 1159
MmeI TCCRAC 4 cut(s) 688, 753, 1187, 1193
Mph1103I ATGCAT 1 cut(s) 1426
MroNI GCCGGC 2 cut(s) 1189, 1302
MseI TTAA 4 cut(s) 579, 741, 756, 1533
MspA1I CMGCKG 5 cut(s) 329, 625, 986, 1231, 1500
MspR9I CCNGG 2 cut(s) 425, 1276
Mva1269I GAATGC 1 cut(s) 252
MvnI CGCG 4 cut(s) 1282, 1361, 1372, 1484
NaeI GCCGGC 2 cut(s) 1191, 1304
NciI CCSGG 2 cut(s) 425, 1276
NdeII GATC 7 cut(s) 247, 427, 469, 718, 854, 1379, 1502
NgoMIV GCCGGC 2 cut(s) 1189, 1302
NlaIII CATG 2 cut(s) 1001, 1450
NlaIV GGNNCC 3 cut(s) 964, 1236, 1504
NmuCI GTSAC 3 cut(s) 480, 837, 955
NsiI ATGCAT 1 cut(s) 1426
NspV TTCGAA 2 cut(s) 507, 784
PauI GCGCGC 1 cut(s) 1359
PcsI WCGNNNNNNNCGW 5 cut(s) 177, 504, 1404, 1410, 1413
PctI GAATGC 1 cut(s) 252
PdiI GCCGGC 2 cut(s) 1191, 1304
PfeI GAWTC 4 cut(s) 317, 323, 383, 586
PinAI ACCGGT 2 cut(s) 396, 959
PleI GAGTC 4 cut(s) 155, 443, 868, 1159
PpsI GAGTC 4 cut(s) 155, 443, 868, 1159
Ppu21I YACGTR 1 cut(s) 1433
PshBI ATTAAT 1 cut(s) 756
Psp124BI GAGCTC 1 cut(s) 1370
PspEI GGTNACC 1 cut(s) 955
PspN4I GGNNCC 3 cut(s) 964, 1236, 1504
PsuI RGATCY 3 cut(s) 427, 1379, 1502
PteI GCGCGC 1 cut(s) 1359
PvuII CAGCTG 1 cut(s) 329
RigI GGCCGGCC 1 cut(s) 1306
RsaI GTAC 4 cut(s) 295, 566, 964, 1467
RsaNI GTAC 4 cut(s) 294, 565, 963, 1466
SacI GAGCTC 1 cut(s) 1370
SaqAI TTAA 4 cut(s) 579, 741, 756, 1533
Sau3AI GATC 7 cut(s) 247, 427, 469, 718, 854, 1379, 1502
ScaI AGTACT 1 cut(s) 566
SchI GAGTC 4 cut(s) 155, 443, 869, 1159
ScrFI CCNGG 2 cut(s) 425, 1276
SduI GDGCHC 1 cut(s) 1370
SfaNI GCATC 6 cut(s) 239, 341, 817, 1411, 1433, 1608
SfuI TTCGAA 2 cut(s) 507, 784
SmlI CTYRAG 1 cut(s) 1583
SmoI CTYRAG 1 cut(s) 1583
SpeI ACTAGT 1 cut(s) 1220
Sse9I AATT 4 cut(s) 101, 510, 576, 843
SspI AATATT 3 cut(s) 268, 289, 1031
SspMI CTAG 3 cut(s) 1221, 1521, 1560
SstI GAGCTC 1 cut(s) 1370
StyD4I CCNGG 2 cut(s) 423, 1274
StyI CCWWGG 1 cut(s) 558
TaaI ACNGT 5 cut(s) 42, 486, 524, 706, 1331
TaiI ACGT 7 cut(s) 174, 698, 823, 1401, 1410, 1419, 1435
TaqI TCGA 5 cut(s) 498, 507, 641, 784, 863
TasI AATT 4 cut(s) 101, 510, 576, 843
TatI WGTACW 2 cut(s) 564, 1465
TfiI GAWTC 4 cut(s) 317, 323, 383, 586
Tru1I TTAA 4 cut(s) 579, 741, 756, 1533
Tru9I TTAA 4 cut(s) 579, 741, 756, 1533
TscAI CASTG 2 cut(s) 819, 1303
TseFI GTSAC 3 cut(s) 480, 837, 955
TseI GCWGC 7 cut(s) 329, 411, 625, 946, 986, 1186, 1497
Tsp45I GTSAC 3 cut(s) 480, 837, 955
TspDTI ATGAA 4 cut(s) 1014, 1463, 1543, 1607
TspGWI ACGGA 5 cut(s) 133, 727, 743, 1138, 1498
TspRI CASTG 2 cut(s) 819, 1303
VspI ATTAAT 1 cut(s) 756
XspI CTAG 3 cut(s) 1221, 1521, 1560
ZrmI AGTACT 1 cut(s) 566
Zsp2I ATGCAT 1 cut(s) 1426
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.