pycom02g09940

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0008807
Physical Location & Seq
Reverse (-)
6867956 .. 6869757
1802 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g09940.4

Sequence Viewer

Length: 786 bp
ATGGGGAAAGTGTCTCCAAAGGAGAGGAATTCGAAGAATCCGAAACAGAAGAAGCGGCGGGGTTCCAGCAGTAAGTATCTGAAGCCGGGTGCTCTTGCCCAGCCTAAGTACAATAAGGTGTCTTCAGGTAAGTCTTGTACTGATCTGGGGAGGAAAAGGGTGGCTGTGTTTGAGAGCAAGAAGGTTGAGGGTGACATGGTGCTCGAAGATAAGGCTATTGATAAGAGCCCATTGATGTTATCCCTTGTGAATTTGGCTCGGCAGAATAGTTTGTTGAAAACTCCGAGGACTCCTCGCTTTGAAGACTGCAAGTCAGAGTCCCGGCTTGAGTCCCTTCCCATGGATTTGCTGGTTAAGTTATTGTGTCACCTGCACCATGACCAACTGAAGGCACTTTTTCACGTTTCTCAGCGGGTTAGAAAAGCTGTTGTAATTGCGAGGCAGTTCTACTTTAATTATACAACTCCAGATCGCTCTAGACAGGAGATGTTGAATACAATGACACCCCATCCCGCAGAGCACTGGCCTTTTCTAAACAAGGGAAATGGGAATGGTGTATGGATTCGGAGCCCCCATACCCCAAAAGCACCGAAGCATGGTCCCCGACCACCATCCCGCCTCAAAGTCTCTGAAATGAGGCAGGTTGCTGCAGTTCTCTTCCAGGAGTCAAATTTCGCCTCAAGATGCATGGTGCCATCTGCCATACCAAAGCCCCTGTGCAAAAATCGAGTTCTGTTCTACGAGGACGAGTTATGCCAGGCTGTTGCTCAGAACAAACTTCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

262

Amino Acids

29.68

Weight (kDa)

10.14

Isoelectric Point (pI)

45.07

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 378
Acc36I ACCTGC 2 cut(s) 378, 631
AccB1I GGYRCC 1 cut(s) 691
AciI CCGC 5 cut(s) 55, 58, 412, 513, 616
AcsI RAATTY 3 cut(s) 28, 250, 670
AcuI CTGAAG 3 cut(s) 101, 108, 407
AfaI GTAC 2 cut(s) 110, 139
AfiI CCNNNNNNNGG 3 cut(s) 340, 388, 596
AgsI TTSAA 3 cut(s) 277, 302, 493
AhdI GACNNNNNGTC 1 cut(s) 310
AjnI CCWGG 2 cut(s) 660, 756
AluBI AGCT 1 cut(s) 425
AluI AGCT 1 cut(s) 425
Alw21I GWGCWC 3 cut(s) 94, 204, 522
Alw26I GTCTC 2 cut(s) 18, 631
AoxI GGCC 1 cut(s) 524
ApeKI GCWGC 1 cut(s) 647
ApoI RAATTY 3 cut(s) 28, 250, 670
AspS9I GGNCC 1 cut(s) 599
AsuC2I CCSGG 2 cut(s) 87, 322
AsuHPI GGTGA 2 cut(s) 203, 359
AsuII TTCGAA 1 cut(s) 32
AvaII GGWCC 1 cut(s) 599
BanI GGYRCC 1 cut(s) 691
BanII GRGCYC 2 cut(s) 230, 572
BbsI GAAGAC 2 cut(s) 114, 309
Bbv12I GWGCWC 3 cut(s) 94, 204, 522
BbvI GCAGC 1 cut(s) 634
BccI CCATC 3 cut(s) 516, 619, 703
BciT130I CCWGG 2 cut(s) 662, 758
BcnI CCSGG 2 cut(s) 87, 322
BcoDI GTCTC 2 cut(s) 18, 631
BfaI CTAG 1 cut(s) 477
BfmI CTRYAG 1 cut(s) 648
BfuAI ACCTGC 2 cut(s) 378, 631
BisI GCNGC 2 cut(s) 56, 648
BlsI GCNGC 2 cut(s) 57, 649
Bme1390I CCNGG 4 cut(s) 87, 322, 662, 758
Bme18I GGWCC 1 cut(s) 599
BmeRI GACNNNNNGTC 1 cut(s) 310
BmgT120I GGNCC 1 cut(s) 599
BmiI GGNNCC 4 cut(s) 64, 569, 601, 693
BmrFI CCNGG 4 cut(s) 87, 322, 662, 758
BmsI GCATC 1 cut(s) 674
BpiI GAAGAC 2 cut(s) 114, 309
BplI GAGNNNNNCTC 2 cut(s) 277, 309
BpmI CTGGAG 1 cut(s) 450
Bpu14I TTCGAA 1 cut(s) 32
BpuEI CTTGAG 2 cut(s) 347, 664
BpuMI CCSGG 2 cut(s) 87, 322
BsaJI CCNNGG 2 cut(s) 284, 339
Bsc4I CCNNNNNNNGG 3 cut(s) 340, 388, 596
Bse1I ACTGG 1 cut(s) 527
BseBI CCWGG 2 cut(s) 662, 758
BseDI CCNNGG 2 cut(s) 284, 339
BseGI GGATG 2 cut(s) 508, 611
BseLI CCNNNNNNNGG 3 cut(s) 340, 388, 596
BseMII CTCAG 2 cut(s) 422, 782
BseNI ACTGG 1 cut(s) 527
BseRI GAGGAG 1 cut(s) 282
BseXI GCAGC 1 cut(s) 634
BseYI CCCAGC 1 cut(s) 99
BsgI GTGCAG 1 cut(s) 356
BshFI GGCC 1 cut(s) 526
BshNI GGYRCC 1 cut(s) 691
BsiHKAI GWGCWC 3 cut(s) 94, 204, 522
BsiSI CCGG 2 cut(s) 86, 322
BslFI GGGAC 3 cut(s) 304, 316, 585
BslI CCNNNNNNNGG 3 cut(s) 340, 388, 596
BsmAI GTCTC 2 cut(s) 18, 631
BsmFI GGGAC 3 cut(s) 304, 316, 585
BsnI GGCC 1 cut(s) 526
Bsp119I TTCGAA 1 cut(s) 32
Bsp1286I GDGCHC 5 cut(s) 94, 204, 230, 522, 572
Bsp143I GATC 2 cut(s) 142, 469
Bsp19I CCATGG 1 cut(s) 339
BspACI CCGC 5 cut(s) 55, 58, 412, 513, 616
BspANI GGCC 1 cut(s) 526
BspCNI CTCAG 2 cut(s) 421, 781
BspLI GGNNCC 4 cut(s) 64, 569, 601, 693
BspMAI CTGCAG 1 cut(s) 652
BspMI ACCTGC 2 cut(s) 378, 631
BspT104I TTCGAA 1 cut(s) 32
BspT107I GGYRCC 1 cut(s) 691
BsrI ACTGG 1 cut(s) 527
BssECI CCNNGG 2 cut(s) 284, 339
BssMI GATC 2 cut(s) 142, 469
BssT1I CCWWGG 1 cut(s) 339
Bst2UI CCWGG 2 cut(s) 662, 758
Bst6I CTCTTC 1 cut(s) 662
BstBI TTCGAA 1 cut(s) 32
BstDEI CTNAG 3 cut(s) 105, 408, 768
BstDSI CCRYGG 1 cut(s) 339
BstF5I GGATG 2 cut(s) 508, 611
BstKTI GATC 2 cut(s) 145, 472
BstMAI GTCTC 2 cut(s) 18, 631
BstMBI GATC 2 cut(s) 142, 469
BstNI CCWGG 2 cut(s) 662, 758
BstSCI CCNGG 4 cut(s) 85, 320, 660, 756
BstSFI CTRYAG 1 cut(s) 648
BstV1I GCAGC 1 cut(s) 634
BstV2I GAAGAC 2 cut(s) 114, 309
BsuRI GGCC 1 cut(s) 526
BtgI CCRYGG 1 cut(s) 339
BtsCI GGATG 2 cut(s) 508, 611
BtsIMutI CAGTG 1 cut(s) 520
BveI ACCTGC 2 cut(s) 378, 631
Cfr13I GGNCC 1 cut(s) 599
Csp6I GTAC 2 cut(s) 109, 138
CviAII CATG 5 cut(s) 196, 340, 377, 596, 688
CviQI GTAC 2 cut(s) 109, 138
DdeI CTNAG 3 cut(s) 105, 408, 768
DpnI GATC 2 cut(s) 144, 471
DpnII GATC 2 cut(s) 142, 469
DriI GACNNNNNGTC 1 cut(s) 310
Eam1104I CTCTTC 1 cut(s) 662
Eam1105I GACNNNNNGTC 1 cut(s) 310
EarI CTCTTC 1 cut(s) 662
Eco130I CCWWGG 1 cut(s) 339
Eco24I GRGCYC 2 cut(s) 230, 572
Eco47I GGWCC 1 cut(s) 599
Eco57I CTGAAG 3 cut(s) 101, 108, 407
EcoRI GAATTC 1 cut(s) 28
EcoRII CCWGG 2 cut(s) 660, 756
EcoT14I CCWWGG 1 cut(s) 339
EcoT22I ATGCAT 1 cut(s) 689
EcoT38I GRGCYC 2 cut(s) 230, 572
ErhI CCWWGG 1 cut(s) 339
FaeI CATG 5 cut(s) 199, 343, 380, 599, 691
FaqI GGGAC 3 cut(s) 304, 316, 585
FatI CATG 5 cut(s) 195, 339, 376, 595, 687
FauI CCCGC 4 cut(s) 51, 405, 520, 623
Fnu4HI GCNGC 2 cut(s) 56, 648
FokI GGATG 2 cut(s) 495, 598
FriOI GRGCYC 2 cut(s) 230, 572
Fsp4HI GCNGC 2 cut(s) 56, 648
FspBI CTAG 1 cut(s) 477
GluI GCNGC 2 cut(s) 56, 648
GsaI CCCAGC 1 cut(s) 103
GsuI CTGGAG 1 cut(s) 450
HaeIII GGCC 1 cut(s) 526
HapII CCGG 2 cut(s) 86, 322
Hin1II CATG 5 cut(s) 199, 343, 380, 599, 691
HinfI GANTC 6 cut(s) 37, 289, 317, 329, 562, 665
HpaII CCGG 2 cut(s) 86, 322
HphI GGTGA 2 cut(s) 203, 359
Hpy188I TCNGA 7 cut(s) 42, 81, 285, 316, 567, 631, 771
Hpy188III TCNNGA 3 cut(s) 467, 477, 681
HpyAV CCTTC 3 cut(s) 175, 344, 382
HpyCH4IV ACGT 1 cut(s) 402
HpyCH4V TGCA 5 cut(s) 309, 373, 650, 687, 720
HpyF3I CTNAG 3 cut(s) 105, 408, 768
HpySE526I ACGT 1 cut(s) 402
Hsp92II CATG 5 cut(s) 199, 343, 380, 599, 691
Kzo9I GATC 2 cut(s) 142, 469
LmnI GCTCC 1 cut(s) 567
Lsp1109I GCAGC 1 cut(s) 634
LweI GCATC 1 cut(s) 674
MaeI CTAG 1 cut(s) 477
MaeII ACGT 1 cut(s) 402
MaeIII GTNAC 2 cut(s) 191, 365
MalI GATC 2 cut(s) 144, 471
MboI GATC 2 cut(s) 142, 469
MboII GAAGA 6 cut(s) 46, 61, 114, 218, 314, 649
MhlI GDGCHC 5 cut(s) 94, 204, 230, 522, 572
MluCI AATT 5 cut(s) 28, 250, 432, 454, 670
MlyI GAGTC 4 cut(s) 283, 326, 338, 674
Mph1103I ATGCAT 1 cut(s) 689
MseI TTAA 2 cut(s) 354, 453
MspA1I CMGCKG 1 cut(s) 412
MspI CCGG 2 cut(s) 86, 322
MspR9I CCNGG 4 cut(s) 87, 322, 662, 758
MvaI CCWGG 2 cut(s) 662, 758
NciI CCSGG 2 cut(s) 87, 322
NcoI CCATGG 1 cut(s) 339
NdeII GATC 2 cut(s) 142, 469
NlaIII CATG 5 cut(s) 199, 343, 380, 599, 691
NlaIV GGNNCC 4 cut(s) 64, 569, 601, 693
NmeAIII GCCGAG 1 cut(s) 238
NmuCI GTSAC 2 cut(s) 191, 365
NsiI ATGCAT 1 cut(s) 689
NspV TTCGAA 1 cut(s) 32
PaqCI CACCTGC 1 cut(s) 378
PcsI WCGNNNNNNNCGW 1 cut(s) 38
PfeI GAWTC 2 cut(s) 37, 562
PfoI TCCNGGA 1 cut(s) 660
PkrI GCNGC 2 cut(s) 57, 649
PleI GAGTC 4 cut(s) 283, 325, 337, 673
PpsI GAGTC 4 cut(s) 283, 325, 337, 673
Psp6I CCWGG 2 cut(s) 660, 756
PspFI CCCAGC 1 cut(s) 99
PspGI CCWGG 2 cut(s) 660, 756
PspN4I GGNNCC 4 cut(s) 64, 569, 601, 693
PspPI GGNCC 1 cut(s) 599
PstI CTGCAG 1 cut(s) 652
RsaI GTAC 2 cut(s) 110, 139
RsaNI GTAC 2 cut(s) 109, 138
SaqAI TTAA 2 cut(s) 354, 453
SatI GCNGC 2 cut(s) 56, 648
Sau3AI GATC 2 cut(s) 142, 469
Sau96I GGNCC 1 cut(s) 599
SchI GAGTC 4 cut(s) 283, 326, 338, 674
ScrFI CCNGG 4 cut(s) 87, 322, 662, 758
SduI GDGCHC 5 cut(s) 94, 204, 230, 522, 572
SetI ASST 7 cut(s) 120, 130, 186, 372, 405, 427, 645
SfaNI GCATC 1 cut(s) 674
SfcI CTRYAG 1 cut(s) 648
SfuI TTCGAA 1 cut(s) 32
SinI GGWCC 1 cut(s) 599
SmlI CTYRAG 2 cut(s) 326, 679
SmoI CTYRAG 2 cut(s) 326, 679
Sse9I AATT 5 cut(s) 28, 250, 432, 454, 670
SsiI CCGC 5 cut(s) 55, 58, 412, 513, 616
SspMI CTAG 1 cut(s) 477
StyD4I CCNGG 4 cut(s) 85, 320, 660, 756
StyI CCWWGG 1 cut(s) 339
TaiI ACGT 1 cut(s) 405
TaqI TCGA 3 cut(s) 32, 204, 727
TasI AATT 5 cut(s) 28, 250, 432, 454, 670
TatI WGTACW 2 cut(s) 108, 137
TauI GCSGC 1 cut(s) 58
TfiI GAWTC 2 cut(s) 37, 562
Tru1I TTAA 2 cut(s) 354, 453
Tru9I TTAA 2 cut(s) 354, 453
TscAI CASTG 1 cut(s) 527
TseFI GTSAC 2 cut(s) 191, 365
TseI GCWGC 1 cut(s) 647
Tsp45I GTSAC 2 cut(s) 191, 365
TspRI CASTG 1 cut(s) 527
VpaK11BI GGWCC 1 cut(s) 599
XapI RAATTY 3 cut(s) 28, 250, 670
XbaI TCTAGA 1 cut(s) 476
XcmI CCANNNNNNNNNTGG 1 cut(s) 346
XspI CTAG 1 cut(s) 477
Zsp2I ATGCAT 1 cut(s) 689
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.