Rmu_sc0008564.1_g000002

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0008564.1
OG0008807
Physical Location & Seq
Forward (+)
8565 .. 10164
1600 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0008564.1_g000002.1.cds

Sequence Viewer

Length: 918 bp
atggggaaggtttcgccgaaagagaggggtttgaagaattcgaagcagaagaagggttccaggggctcagggaacaagtatctgaagccgggtgctcttgcccagctaaagtacagcaaggccagttctgctaagccgtgtactgatctggggaggaagcgagtggctgtagtggagacgaagaaggatgatgttgatttggggattgaagatagggctagccctttgatgccgtcgcctgtgaatttggtcaagcagagtagtttgttgaaaacgccgaggactcctttgatgttatcgccggtgaattcggttaagcagactagtttgcttaaaacaccgaggacccctttgatgctgtctcctgtggattttgttaggcagaatagtctgatgaaaacaccaatgacccccagaatggaagattgtgagtccgagtccaggcttgagtcgcttcccatggatttactggttaaattattgtgtcacctgcaccatgaccaactgaagccggtgtttcatgtttctcaaagaattaggaaagctgttatacttgcaaggcagtatcactttaattatacaactccagatcgctctcgacaggagatgttgagaacaatgacaccccaccccactgaacactggcctttcaaaagcaagggagatggcaagggtacatggatacgaagcccgcatactccaaaagcaccaagacatggtcccaggccaccatctcgcctcaaagtttcagaaatgcggcaggttgctgcagttctctttcaggagtcaaatttcccttctagatgcatggtgccatctgccttaccaaagcccctgtgcaaatctttggcttccaatagagttttgttctatgaggaagagttatgtcaggctgtggctcagaataagcttctttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

305

Amino Acids

34.37

Weight (kDa)

10.18

Isoelectric Point (pI)

48.21

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 498
Acc36I ACCTGC 2 cut(s) 498, 751
AccB1I GGYRCC 1 cut(s) 811
AccB7I CCANNNNNTGG 1 cut(s) 716
AciI CCGC 2 cut(s) 692, 757
AcsI RAATTY 4 cut(s) 37, 244, 307, 790
AcuI CTGAAG 2 cut(s) 104, 527
AfaI GTAC 3 cut(s) 113, 142, 676
AfiI CCNNNNNNNGG 3 cut(s) 418, 441, 716
AgsI TTSAA 4 cut(s) 34, 209, 271, 652
AhlI ACTAGT 1 cut(s) 323
AjnI CCWGG 3 cut(s) 59, 440, 722
AluBI AGCT 3 cut(s) 106, 545, 910
AluI AGCT 3 cut(s) 106, 545, 910
Alw21I GWGCWC 1 cut(s) 97
Alw26I GTCTC 2 cut(s) 170, 366
AoxI GGCC 3 cut(s) 120, 644, 725
ApeKI GCWGC 1 cut(s) 767
ApoI RAATTY 4 cut(s) 37, 244, 307, 790
AspS9I GGNCC 2 cut(s) 345, 719
AsuC2I CCSGG 1 cut(s) 90
AsuHPI GGTGA 2 cut(s) 316, 479
AsuII TTCGAA 1 cut(s) 41
AsuNHI GCTAGC 1 cut(s) 218
AvaII GGWCC 2 cut(s) 345, 719
BanI GGYRCC 1 cut(s) 811
BanII GRGCYC 1 cut(s) 68
BarI GAAGNNNNNNTAC 2 cut(s) 679, 711
Bbv12I GWGCWC 1 cut(s) 97
BbvI GCAGC 1 cut(s) 754
BccI CCATC 3 cut(s) 659, 739, 823
BceAI ACGGC 2 cut(s) 121, 217
BciT130I CCWGG 3 cut(s) 61, 442, 724
BciVI GTATCC 1 cut(s) 675
BcnI CCSGG 1 cut(s) 90
BcoDI GTCTC 2 cut(s) 170, 366
BcuI ACTAGT 1 cut(s) 323
BfaI CTAG 3 cut(s) 219, 324, 801
BfmI CTRYAG 2 cut(s) 168, 768
BfuAI ACCTGC 2 cut(s) 498, 751
BfuI GTATCC 1 cut(s) 675
BisI GCNGC 2 cut(s) 758, 768
BlpI GCTNAGC 1 cut(s) 132
BlsI GCNGC 2 cut(s) 759, 769
Bme1390I CCNGG 4 cut(s) 61, 90, 442, 724
Bme18I GGWCC 2 cut(s) 345, 719
BmgT120I GGNCC 2 cut(s) 345, 719
BmiI GGNNCC 4 cut(s) 58, 347, 721, 813
BmrFI CCNGG 4 cut(s) 61, 90, 442, 724
BmsI GCATC 3 cut(s) 219, 345, 794
BmtI GCTAGC 1 cut(s) 222
BpmI CTGGAG 1 cut(s) 570
Bpu10I CCTNAGC 1 cut(s) 67
Bpu1102I GCTNAGC 1 cut(s) 132
Bpu14I TTCGAA 1 cut(s) 41
BpuEI CTTGAG 1 cut(s) 467
BpuMI CCSGG 1 cut(s) 90
BsaJI CCNNGG 5 cut(s) 60, 278, 341, 459, 722
Bsc4I CCNNNNNNNGG 3 cut(s) 418, 441, 716
Bse118I RCCGGY 2 cut(s) 301, 511
Bse1I ACTGG 3 cut(s) 123, 474, 647
BseBI CCWGG 3 cut(s) 61, 442, 724
BseDI CCNNGG 5 cut(s) 60, 278, 341, 459, 722
BseGI GGATG 1 cut(s) 193
BseLI CCNNNNNNNGG 3 cut(s) 418, 441, 716
BseMII CTCAG 2 cut(s) 81, 914
BseNI ACTGG 3 cut(s) 123, 474, 647
BseXI GCAGC 1 cut(s) 754
BseYI CCCAGC 1 cut(s) 102
BsgI GTGCAG 1 cut(s) 476
BshFI GGCC 3 cut(s) 122, 646, 727
BshNI GGYRCC 1 cut(s) 811
BsiHKAI GWGCWC 1 cut(s) 97
BsiSI CCGG 3 cut(s) 89, 302, 512
BslFI GGGAC 1 cut(s) 705
BslI CCNNNNNNNGG 3 cut(s) 418, 441, 716
BsmAI GTCTC 2 cut(s) 170, 366
BsmBI CGTCTC 1 cut(s) 170
BsmFI GGGAC 1 cut(s) 705
BsnI GGCC 3 cut(s) 122, 646, 727
Bsp119I TTCGAA 1 cut(s) 41
Bsp1286I GDGCHC 2 cut(s) 68, 97
Bsp143I GATC 2 cut(s) 145, 589
Bsp1720I GCTNAGC 1 cut(s) 132
Bsp19I CCATGG 1 cut(s) 459
BspACI CCGC 2 cut(s) 692, 757
BspANI GGCC 3 cut(s) 122, 646, 727
BspCNI CTCAG 2 cut(s) 80, 913
BspLI GGNNCC 4 cut(s) 58, 347, 721, 813
BspMAI CTGCAG 1 cut(s) 772
BspMI ACCTGC 2 cut(s) 498, 751
BspOI GCTAGC 1 cut(s) 222
BspT104I TTCGAA 1 cut(s) 41
BspT107I GGYRCC 1 cut(s) 811
BsrFI RCCGGY 2 cut(s) 301, 511
BsrI ACTGG 3 cut(s) 123, 474, 647
BssAI RCCGGY 2 cut(s) 301, 511
BssECI CCNNGG 5 cut(s) 60, 278, 341, 459, 722
BssMI GATC 2 cut(s) 145, 589
BssT1I CCWWGG 1 cut(s) 459
Bst2UI CCWGG 3 cut(s) 61, 442, 724
Bst6I CTCTTC 1 cut(s) 873
BstBI TTCGAA 1 cut(s) 41
BstC8I GCNNGC 2 cut(s) 220, 692
BstDEI CTNAG 3 cut(s) 67, 132, 900
BstDSI CCRYGG 1 cut(s) 459
BstF5I GGATG 1 cut(s) 193
BstKTI GATC 2 cut(s) 148, 592
BstMAI GTCTC 2 cut(s) 170, 366
BstMBI GATC 2 cut(s) 145, 589
BstMWI GCNNNNNNNGC 2 cut(s) 128, 451
BstNI CCWGG 3 cut(s) 61, 442, 724
BstSCI CCNGG 4 cut(s) 59, 88, 440, 722
BstSFI CTRYAG 2 cut(s) 168, 768
BstV1I GCAGC 1 cut(s) 754
BsuI GTATCC 1 cut(s) 675
BsuRI GGCC 3 cut(s) 122, 646, 727
BtgI CCRYGG 1 cut(s) 459
BtsCI GGATG 1 cut(s) 193
BtsIMutI CAGTG 2 cut(s) 633, 640
BveI ACCTGC 2 cut(s) 498, 751
Cac8I GCNNGC 2 cut(s) 220, 692
Cfr10I RCCGGY 2 cut(s) 301, 511
Cfr13I GGNCC 2 cut(s) 345, 719
Csp6I GTAC 3 cut(s) 112, 141, 675
CviAII CATG 6 cut(s) 460, 497, 521, 678, 716, 808
CviQI GTAC 3 cut(s) 112, 141, 675
DdeI CTNAG 3 cut(s) 67, 132, 900
DpnI GATC 2 cut(s) 147, 591
DpnII GATC 2 cut(s) 145, 589
Eam1104I CTCTTC 1 cut(s) 873
EarI CTCTTC 1 cut(s) 873
Eco130I CCWWGG 1 cut(s) 459
Eco24I GRGCYC 1 cut(s) 68
Eco47I GGWCC 2 cut(s) 345, 719
Eco57I CTGAAG 2 cut(s) 104, 527
EcoO109I RGGNCCY 1 cut(s) 345
EcoRI GAATTC 2 cut(s) 37, 307
EcoRII CCWGG 3 cut(s) 59, 440, 722
EcoT14I CCWWGG 1 cut(s) 459
EcoT22I ATGCAT 1 cut(s) 809
EcoT38I GRGCYC 1 cut(s) 68
ErhI CCWWGG 1 cut(s) 459
Esp3I CGTCTC 1 cut(s) 170
FaeI CATG 6 cut(s) 463, 500, 524, 681, 719, 811
FaqI GGGAC 1 cut(s) 705
FatI CATG 6 cut(s) 459, 496, 520, 677, 715, 807
FauI CCCGC 1 cut(s) 699
Fnu4HI GCNGC 2 cut(s) 758, 768
FokI GGATG 1 cut(s) 200
FriOI GRGCYC 1 cut(s) 68
Fsp4HI GCNGC 2 cut(s) 758, 768
FspBI CTAG 3 cut(s) 219, 324, 801
GluI GCNGC 2 cut(s) 758, 768
GsaI CCCAGC 1 cut(s) 106
GsuI CTGGAG 1 cut(s) 570
HaeIII GGCC 3 cut(s) 122, 646, 727
HapII CCGG 3 cut(s) 89, 302, 512
Hin1II CATG 6 cut(s) 463, 500, 524, 681, 719, 811
HindIII AAGCTT 1 cut(s) 908
HinfI GANTC 5 cut(s) 283, 431, 437, 449, 785
HpaII CCGG 3 cut(s) 89, 302, 512
HphI GGTGA 2 cut(s) 316, 479
Hpy166II GTNNAC 1 cut(s) 141
Hpy188I TCNGA 5 cut(s) 84, 393, 436, 751, 903
Hpy188III TCNNGA 4 cut(s) 587, 597, 782, 801
Hpy8I GTNNAC 1 cut(s) 141
Hpy99I CGWCG 1 cut(s) 238
HpyAV CCTTC 3 cut(s) 46, 178, 807
HpyCH4V TGCA 5 cut(s) 493, 557, 770, 807, 840
HpyF10VI GCNNNNNNNGC 2 cut(s) 128, 451
HpyF3I CTNAG 3 cut(s) 67, 132, 900
Hsp92II CATG 6 cut(s) 463, 500, 524, 681, 719, 811
Kzo9I GATC 2 cut(s) 145, 589
Lsp1109I GCAGC 1 cut(s) 754
LweI GCATC 3 cut(s) 219, 345, 794
MaeI CTAG 3 cut(s) 219, 324, 801
MaeIII GTNAC 1 cut(s) 485
MalI GATC 2 cut(s) 147, 591
MboI GATC 2 cut(s) 145, 589
MboII GAAGA 6 cut(s) 46, 61, 193, 221, 434, 890
MhlI GDGCHC 2 cut(s) 68, 97
MluCI AATT 7 cut(s) 37, 244, 307, 476, 534, 574, 790
MlyI GAGTC 5 cut(s) 277, 440, 446, 458, 794
MnlI CCTC 6 cut(s) 18, 147, 273, 336, 749, 868
Mph1103I ATGCAT 1 cut(s) 809
MseI TTAA 5 cut(s) 315, 333, 474, 573, 916
MspI CCGG 3 cut(s) 89, 302, 512
MspR9I CCNGG 4 cut(s) 61, 90, 442, 724
MvaI CCWGG 3 cut(s) 61, 442, 724
MwoI GCNNNNNNNGC 2 cut(s) 128, 451
NciI CCSGG 1 cut(s) 90
NcoI CCATGG 1 cut(s) 459
NdeII GATC 2 cut(s) 145, 589
NheI GCTAGC 1 cut(s) 218
NlaIII CATG 6 cut(s) 463, 500, 524, 681, 719, 811
NlaIV GGNNCC 4 cut(s) 58, 347, 721, 813
NmeAIII GCCGAG 1 cut(s) 303
NmuCI GTSAC 1 cut(s) 485
NsiI ATGCAT 1 cut(s) 809
NspV TTCGAA 1 cut(s) 41
PaqCI CACCTGC 1 cut(s) 498
PflFI GACNNNGTC 1 cut(s) 717
PflMI CCANNNNNTGG 1 cut(s) 716
PkrI GCNGC 2 cut(s) 759, 769
PleI GAGTC 5 cut(s) 277, 439, 445, 457, 793
PpsI GAGTC 5 cut(s) 277, 439, 445, 457, 793
PpuMI RGGWCCY 1 cut(s) 345
Psp5II RGGWCCY 1 cut(s) 345
Psp6I CCWGG 3 cut(s) 59, 440, 722
PspFI CCCAGC 1 cut(s) 102
PspGI CCWGG 3 cut(s) 59, 440, 722
PspN4I GGNNCC 4 cut(s) 58, 347, 721, 813
PspPI GGNCC 2 cut(s) 345, 719
PspPPI RGGWCCY 1 cut(s) 345
PstI CTGCAG 1 cut(s) 772
PsyI GACNNNGTC 1 cut(s) 717
RsaI GTAC 3 cut(s) 113, 142, 676
RsaNI GTAC 3 cut(s) 112, 141, 675
SaqAI TTAA 5 cut(s) 315, 333, 474, 573, 916
SatI GCNGC 2 cut(s) 758, 768
Sau3AI GATC 2 cut(s) 145, 589
Sau96I GGNCC 2 cut(s) 345, 719
SchI GAGTC 5 cut(s) 277, 440, 446, 458, 794
ScrFI CCNGG 4 cut(s) 61, 90, 442, 724
SduI GDGCHC 2 cut(s) 68, 97
SetI ASST 6 cut(s) 12, 108, 492, 547, 765, 912
SfaNI GCATC 3 cut(s) 219, 345, 794
SfcI CTRYAG 2 cut(s) 168, 768
SfuI TTCGAA 1 cut(s) 41
SgrAI CRCCGGYG 1 cut(s) 301
SinI GGWCC 2 cut(s) 345, 719
SmlI CTYRAG 1 cut(s) 446
SmoI CTYRAG 1 cut(s) 446
SpeI ACTAGT 1 cut(s) 323
Sse9I AATT 7 cut(s) 37, 244, 307, 476, 534, 574, 790
SsiI CCGC 2 cut(s) 692, 757
SspMI CTAG 3 cut(s) 219, 324, 801
StyD4I CCNGG 4 cut(s) 59, 88, 440, 722
StyI CCWWGG 1 cut(s) 459
TaqI TCGA 2 cut(s) 41, 598
TasI AATT 7 cut(s) 37, 244, 307, 476, 534, 574, 790
TatI WGTACW 2 cut(s) 111, 140
TauI GCSGC 1 cut(s) 760
Tru1I TTAA 5 cut(s) 315, 333, 474, 573, 916
Tru9I TTAA 5 cut(s) 315, 333, 474, 573, 916
TscAI CASTG 2 cut(s) 640, 647
TseFI GTSAC 1 cut(s) 485
TseI GCWGC 1 cut(s) 767
Tsp45I GTSAC 1 cut(s) 485
TspDTI ATGAA 2 cut(s) 410, 509
TspRI CASTG 2 cut(s) 640, 647
Tth111I GACNNNGTC 1 cut(s) 717
Van91I CCANNNNNTGG 1 cut(s) 716
VpaK11BI GGWCC 2 cut(s) 345, 719
XapI RAATTY 4 cut(s) 37, 244, 307, 790
XbaI TCTAGA 1 cut(s) 800
XcmI CCANNNNNNNNNTGG 1 cut(s) 466
XspI CTAG 3 cut(s) 219, 324, 801
Zsp2I ATGCAT 1 cut(s) 809
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.