pycom15g21300

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0008807
Physical Location & Seq
Forward (+)
15314616 .. 15316498
1883 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g21300.3

Sequence Viewer

Length: 786 bp
ATGGGGAAAGTGTCTCCAAAGGAGAGGAATTCGAAGAACCCGAAACAGAAGAAGCGGCGGGGTTCCGGCAACAAGTATCTGAAACCGGGTGCTCTTGCCCAGCTTAAGTACAATAAGGTGTCTTCAGCCAAGTCTTGTACTGATCTGGGGTGGAAAAGGGTGGCTGCCTTTGAGAGCAAGAGAGTTGAGGGCGATATGGTGATTGAAGATAAGGCTATCGATAAAAGCCCTTTGATGTTGTCCCCTGTGAATTTGGTTAGACAGAATAGTTTGTTGAAAACTCCAAAGACTCCTCGAGTTGAAGATTGCGAGTCGGAGTCCCGGCTTGAGTCCCTTCCCATGGATTTGCTGGTTAAATTATTGTGTCACCTGCATCACGACCAACTGAAGTCGGTTTTTCACGTTTCTCAGCGAGTTAGAAAAGCTGTTGTAATTGCAAGGCAGTTCTACTTCAATTATACAACTCCAGATCGCTCCAGACAGGAGATGCTGAATACAATGACACCCCATCCCGCAGAGCACTGGCCTTTTCTTAACAAGGGAGATGGGAAAGGTACATGGATTCGGAGCCCCCATACTCCAAAAGCACCAAAGCATGGTCCCCGGCCACCATCCCGCCTCAAAGTCTCAGAAATGAGGCAGGTTGCAGCAGTTCTCTTCCAGGAGTCAAATTTCCATCCAAGATGCATGATGCCATCTGCCTTACCAAAGCCCCTGTGCAAAAATCGAGTTCTGTTCTACGAGGACGAGTTATGTCAGGCTGTTGCTCAGAACAAACTTCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

262

Amino Acids

29.8

Weight (kDa)

9.99

Isoelectric Point (pI)

51.41

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 378
Acc36I ACCTGC 2 cut(s) 378, 631
AccB7I CCANNNNNTGG 1 cut(s) 596
AciI CCGC 4 cut(s) 55, 58, 513, 616
AcoI YGGCCR 1 cut(s) 605
AcsI RAATTY 3 cut(s) 28, 250, 670
AcuI CTGAAG 2 cut(s) 108, 407
AfaI GTAC 3 cut(s) 110, 139, 556
AfiI CCNNNNNNNGG 2 cut(s) 340, 596
AflII CTTAAG 1 cut(s) 104
AgsI TTSAA 4 cut(s) 206, 277, 302, 454
AjnI CCWGG 1 cut(s) 660
AluBI AGCT 2 cut(s) 103, 425
AluI AGCT 2 cut(s) 103, 425
Alw21I GWGCWC 2 cut(s) 94, 522
Alw26I GTCTC 2 cut(s) 18, 631
Ama87I CYCGRG 1 cut(s) 294
AoxI GGCC 2 cut(s) 524, 605
ApeKI GCWGC 2 cut(s) 164, 647
ApoI RAATTY 3 cut(s) 28, 250, 670
AspS9I GGNCC 1 cut(s) 599
AsuC2I CCSGG 3 cut(s) 87, 322, 604
AsuHPI GGTGA 2 cut(s) 211, 359
AsuII TTCGAA 1 cut(s) 32
AvaI CYCGRG 1 cut(s) 294
AvaII GGWCC 1 cut(s) 599
BanII GRGCYC 1 cut(s) 572
BbsI GAAGAC 1 cut(s) 114
Bbv12I GWGCWC 2 cut(s) 94, 522
BbvI GCAGC 2 cut(s) 151, 659
BccI CCATC 5 cut(s) 516, 539, 619, 684, 703
BciT130I CCWGG 1 cut(s) 662
BcnI CCSGG 3 cut(s) 87, 322, 604
BcoDI GTCTC 2 cut(s) 18, 631
BfrI CTTAAG 1 cut(s) 104
BfuAI ACCTGC 2 cut(s) 378, 631
BisI GCNGC 3 cut(s) 56, 165, 648
BlsI GCNGC 3 cut(s) 57, 166, 649
Bme1390I CCNGG 4 cut(s) 87, 322, 604, 662
Bme18I GGWCC 1 cut(s) 599
BmeT110I CYCGRG 1 cut(s) 294
BmgT120I GGNCC 1 cut(s) 599
BmiI GGNNCC 3 cut(s) 64, 569, 601
BmrFI CCNGG 4 cut(s) 87, 322, 604, 662
BmsI GCATC 4 cut(s) 382, 477, 674, 681
BpiI GAAGAC 1 cut(s) 114
BpmI CTGGAG 2 cut(s) 450, 460
Bpu14I TTCGAA 1 cut(s) 32
BpuEI CTTGAG 1 cut(s) 347
BpuMI CCSGG 3 cut(s) 87, 322, 604
Bsa29I ATCGAT 1 cut(s) 219
BsaJI CCNNGG 2 cut(s) 339, 602
Bsc4I CCNNNNNNNGG 2 cut(s) 340, 596
Bse1I ACTGG 1 cut(s) 527
BseBI CCWGG 1 cut(s) 662
BseCI ATCGAT 1 cut(s) 219
BseDI CCNNGG 2 cut(s) 339, 602
BseGI GGATG 3 cut(s) 508, 611, 676
BseLI CCNNNNNNNGG 2 cut(s) 340, 596
BseMII CTCAG 3 cut(s) 422, 642, 782
BseNI ACTGG 1 cut(s) 527
BseRI GAGGAG 1 cut(s) 282
BseXI GCAGC 2 cut(s) 151, 659
BseYI CCCAGC 1 cut(s) 99
BshFI GGCC 2 cut(s) 526, 607
BshVI ATCGAT 1 cut(s) 219
BsiHKAI GWGCWC 2 cut(s) 94, 522
BsiHKCI CYCGRG 1 cut(s) 294
BsiSI CCGG 4 cut(s) 66, 86, 322, 604
BslFI GGGAC 4 cut(s) 226, 304, 316, 585
BslI CCNNNNNNNGG 2 cut(s) 340, 596
BsmAI GTCTC 2 cut(s) 18, 631
BsmFI GGGAC 4 cut(s) 226, 304, 316, 585
BsnI GGCC 2 cut(s) 526, 607
BsoBI CYCGRG 1 cut(s) 294
Bsp119I TTCGAA 1 cut(s) 32
Bsp1286I GDGCHC 3 cut(s) 94, 522, 572
Bsp143I GATC 2 cut(s) 142, 469
Bsp19I CCATGG 1 cut(s) 339
BspACI CCGC 4 cut(s) 55, 58, 513, 616
BspANI GGCC 2 cut(s) 526, 607
BspCNI CTCAG 3 cut(s) 421, 641, 781
BspDI ATCGAT 1 cut(s) 219
BspLI GGNNCC 3 cut(s) 64, 569, 601
BspMI ACCTGC 2 cut(s) 378, 631
BspT104I TTCGAA 1 cut(s) 32
BspTI CTTAAG 1 cut(s) 104
BsrI ACTGG 1 cut(s) 527
BssECI CCNNGG 2 cut(s) 339, 602
BssMI GATC 2 cut(s) 142, 469
BssT1I CCWWGG 1 cut(s) 339
Bst2UI CCWGG 1 cut(s) 662
Bst6I CTCTTC 1 cut(s) 662
BstAFI CTTAAG 1 cut(s) 104
BstBI TTCGAA 1 cut(s) 32
BstDEI CTNAG 3 cut(s) 408, 628, 768
BstDSI CCRYGG 1 cut(s) 339
BstF5I GGATG 3 cut(s) 508, 611, 676
BstKTI GATC 2 cut(s) 145, 472
BstMAI GTCTC 2 cut(s) 18, 631
BstMBI GATC 2 cut(s) 142, 469
BstNI CCWGG 1 cut(s) 662
BstSCI CCNGG 4 cut(s) 85, 320, 602, 660
BstV1I GCAGC 2 cut(s) 151, 659
BstV2I GAAGAC 1 cut(s) 114
Bsu15I ATCGAT 1 cut(s) 219
BsuRI GGCC 2 cut(s) 526, 607
BsuTUI ATCGAT 1 cut(s) 219
BtgI CCRYGG 1 cut(s) 339
BtsCI GGATG 3 cut(s) 508, 611, 676
BtsIMutI CAGTG 1 cut(s) 520
BveI ACCTGC 2 cut(s) 378, 631
Cfr13I GGNCC 1 cut(s) 599
ClaI ATCGAT 1 cut(s) 219
Csp6I GTAC 3 cut(s) 109, 138, 555
CviAII CATG 4 cut(s) 340, 558, 596, 688
CviQI GTAC 3 cut(s) 109, 138, 555
DdeI CTNAG 3 cut(s) 408, 628, 768
DpnI GATC 2 cut(s) 144, 471
DpnII GATC 2 cut(s) 142, 469
EaeI YGGCCR 1 cut(s) 605
Eam1104I CTCTTC 1 cut(s) 662
EarI CTCTTC 1 cut(s) 662
Eco130I CCWWGG 1 cut(s) 339
Eco24I GRGCYC 1 cut(s) 572
Eco47I GGWCC 1 cut(s) 599
Eco57I CTGAAG 2 cut(s) 108, 407
Eco88I CYCGRG 1 cut(s) 294
EcoRI GAATTC 1 cut(s) 28
EcoRII CCWGG 1 cut(s) 660
EcoT14I CCWWGG 1 cut(s) 339
EcoT22I ATGCAT 1 cut(s) 689
EcoT38I GRGCYC 1 cut(s) 572
ErhI CCWWGG 1 cut(s) 339
FaeI CATG 4 cut(s) 343, 561, 599, 691
FaiI YATR 8 cut(s) 197, 341, 459, 559, 576, 597, 689, 754
FaqI GGGAC 4 cut(s) 226, 304, 316, 585
FatI CATG 4 cut(s) 339, 557, 595, 687
FauI CCCGC 3 cut(s) 51, 520, 623
Fnu4HI GCNGC 3 cut(s) 56, 165, 648
FokI GGATG 3 cut(s) 495, 598, 663
FriOI GRGCYC 1 cut(s) 572
Fsp4HI GCNGC 3 cut(s) 56, 165, 648
GluI GCNGC 3 cut(s) 56, 165, 648
GsaI CCCAGC 1 cut(s) 103
GsuI CTGGAG 2 cut(s) 450, 460
HaeIII GGCC 2 cut(s) 526, 607
HapII CCGG 4 cut(s) 66, 86, 322, 604
Hin1II CATG 4 cut(s) 343, 561, 599, 691
HinfI GANTC 6 cut(s) 289, 311, 317, 329, 562, 665
HpaII CCGG 4 cut(s) 66, 86, 322, 604
HphI GGTGA 2 cut(s) 211, 359
Hpy188I TCNGA 5 cut(s) 81, 316, 567, 631, 771
Hpy188III TCNNGA 3 cut(s) 377, 467, 477
HpyAV CCTTC 1 cut(s) 344
HpyCH4IV ACGT 1 cut(s) 402
HpyCH4V TGCA 5 cut(s) 373, 437, 647, 687, 720
HpyF3I CTNAG 3 cut(s) 408, 628, 768
HpySE526I ACGT 1 cut(s) 402
Hsp92II CATG 4 cut(s) 343, 561, 599, 691
Kzo9I GATC 2 cut(s) 142, 469
LmnI GCTCC 2 cut(s) 479, 567
Lsp1109I GCAGC 2 cut(s) 151, 659
LweI GCATC 4 cut(s) 382, 477, 674, 681
MaeII ACGT 1 cut(s) 402
MaeIII GTNAC 1 cut(s) 365
MalI GATC 2 cut(s) 144, 471
MboI GATC 2 cut(s) 142, 469
MboII GAAGA 6 cut(s) 46, 61, 114, 218, 314, 649
MhlI GDGCHC 3 cut(s) 94, 522, 572
MluCI AATT 6 cut(s) 28, 250, 356, 432, 454, 670
MlyI GAGTC 5 cut(s) 283, 320, 326, 338, 674
MmeI TCCRAC 1 cut(s) 294
MnlI CCTC 6 cut(s) 18, 181, 303, 629, 630, 736
Mph1103I ATGCAT 1 cut(s) 689
MseI TTAA 3 cut(s) 105, 354, 534
MspCI CTTAAG 1 cut(s) 104
MspI CCGG 4 cut(s) 66, 86, 322, 604
MspR9I CCNGG 4 cut(s) 87, 322, 604, 662
MvaI CCWGG 1 cut(s) 662
NciI CCSGG 3 cut(s) 87, 322, 604
NcoI CCATGG 1 cut(s) 339
NdeII GATC 2 cut(s) 142, 469
NlaIII CATG 4 cut(s) 343, 561, 599, 691
NlaIV GGNNCC 3 cut(s) 64, 569, 601
NmuCI GTSAC 1 cut(s) 365
NsiI ATGCAT 1 cut(s) 689
NspV TTCGAA 1 cut(s) 32
PaeR7I CTCGAG 1 cut(s) 294
PaqCI CACCTGC 1 cut(s) 378
PcsI WCGNNNNNNNCGW 1 cut(s) 38
PfeI GAWTC 1 cut(s) 562
PflMI CCANNNNNTGG 1 cut(s) 596
PfoI TCCNGGA 1 cut(s) 660
PkrI GCNGC 3 cut(s) 57, 166, 649
PleI GAGTC 5 cut(s) 283, 319, 325, 337, 673
PpsI GAGTC 5 cut(s) 283, 319, 325, 337, 673
Psp6I CCWGG 1 cut(s) 660
PspFI CCCAGC 1 cut(s) 99
PspGI CCWGG 1 cut(s) 660
PspN4I GGNNCC 3 cut(s) 64, 569, 601
PspPI GGNCC 1 cut(s) 599
PspXI VCTCGAGB 1 cut(s) 294
RsaI GTAC 3 cut(s) 110, 139, 556
RsaNI GTAC 3 cut(s) 109, 138, 555
SaqAI TTAA 3 cut(s) 105, 354, 534
SatI GCNGC 3 cut(s) 56, 165, 648
Sau3AI GATC 2 cut(s) 142, 469
Sau96I GGNCC 1 cut(s) 599
SchI GAGTC 5 cut(s) 283, 320, 326, 338, 674
ScrFI CCNGG 4 cut(s) 87, 322, 604, 662
SduI GDGCHC 3 cut(s) 94, 522, 572
SetI ASST 7 cut(s) 105, 120, 372, 405, 427, 556, 645
SfaNI GCATC 4 cut(s) 382, 477, 674, 681
Sfr274I CTCGAG 1 cut(s) 294
SfuI TTCGAA 1 cut(s) 32
SinI GGWCC 1 cut(s) 599
SlaI CTCGAG 1 cut(s) 294
SmlI CTYRAG 3 cut(s) 104, 294, 326
SmoI CTYRAG 3 cut(s) 104, 294, 326
Sse9I AATT 6 cut(s) 28, 250, 356, 432, 454, 670
SsiI CCGC 4 cut(s) 55, 58, 513, 616
StyD4I CCNGG 4 cut(s) 85, 320, 602, 660
StyI CCWWGG 1 cut(s) 339
TaiI ACGT 1 cut(s) 405
TaqI TCGA 4 cut(s) 32, 219, 295, 727
TasI AATT 6 cut(s) 28, 250, 356, 432, 454, 670
TatI WGTACW 2 cut(s) 108, 137
TauI GCSGC 1 cut(s) 58
TfiI GAWTC 1 cut(s) 562
Tru1I TTAA 3 cut(s) 105, 354, 534
Tru9I TTAA 3 cut(s) 105, 354, 534
TscAI CASTG 1 cut(s) 527
TseFI GTSAC 1 cut(s) 365
TseI GCWGC 2 cut(s) 164, 647
Tsp45I GTSAC 1 cut(s) 365
TspRI CASTG 1 cut(s) 527
Van91I CCANNNNNTGG 1 cut(s) 596
Vha464I CTTAAG 1 cut(s) 104
VpaK11BI GGWCC 1 cut(s) 599
XapI RAATTY 3 cut(s) 28, 250, 670
XcmI CCANNNNNNNNNTGG 1 cut(s) 346
XhoI CTCGAG 1 cut(s) 294
Zsp2I ATGCAT 1 cut(s) 689
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.