pycom02g12710

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0015376
Physical Location & Seq
Forward (+)
9281265 .. 9283641
2377 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1287 bp
ATGACTGTCTATGATTCTATGACTATAAATCTTACAAGTTTTCACCGATGGCTTCTTTTAGCTTCTCTCGTAGCAAGAAGCTGCTGCATATATAGTTACTTTTTAGGACTTAGTTTTCATATCATCGACTTTGTGAAGCTTCAAAACTGCATGGCCATGGAGTATTCTTACACTGTGGCCTGCGTAGGGTTTGTTCTTTTGATCGTTTTTAATCTGAGTTATTTGGGAAATGCTGCTGAGAATCAAGGGTTAGGAGCTTCTTTTATCTTTGGTGATTCTCTGGTTGATGCTGGAAACAACAACTATTTGCCCACGTTGTCGAAGGCCAATATGGTTCCGAACGGGATTGATTTCAAGGCGTCTGGAGGAAAACCAACTGGAAGGTTTACCAATGGCAGAACCATTGGTGATATTGTTGGAGAAGAATTGGGGCAACCAAAGTATGCACTCCCATATTTGTCACCAGATGCCAAAGGGAAAGCCCTATTGGTTGGGGTGAATTATGCATCAGGAGCAGCAGGGATTATGAATGCAACAGGAAGAATATTTGTTAATAGGGTGGGAATGGATATCCAAGTTGATTTTTTCAACCAGACAAGGATGCAGATCGACAACGTATTGGGTCCATCACAGGCAAAAGAGTACATTATGAAAAGATCTATTTTTTCAGTCACAATTGGGTCCAATGACTTTTTGAACAATTATCTACTACCAGTCCTCTACATTGGAGCAAGAATTTCTCAGAGCCCAGATGCTTTTATTGATGATTTGATCTCTCACTTCAGAGTCCAACTTACTAGACTTTATCAATTGGATGCTCGCAAATTTGTGATTGGAAATGTTGGACCTATTGGATGCATACCTTACCAAAAGACCATAAATCAACTGAATCCAGACCAGTGTGTGGATTTGCCAAATAAGCTAGCTCTTCAGTACAATGGGCGGTTGAAGGGCTTACTTGCCGAACTGAGTGAGAACCTTCCAGGATCAACATTTGTTTATGCAAACGTGTATGATGTAGTGATGGATGTCATTACAAATTATGACAAATATGGATTTACAACAGCAAGTGTAGCATGCTGTGGAATTGGTGGCCAATACGCAGGTATAATTCCATGTGGTCCACAATCCAGTCTCTGCAAGGATCGGTCGATGCATGTTTTCTGGGATCCCTACCATCCAAGTGAAGCTGCCAACCTTATACTTGCCAAAAAATTGCTTGATGGAGACACAAGAATCATTTCTCCAATGAATCTCAGACAACTCAGAGACCATGGATTAGGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

429

Amino Acids

46.98

Weight (kDa)

7.5

Isoelectric Point (pI)

29.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 88 - 406 1.7e-31 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015376)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G23540
fragaria_vesca FvH4_1g14780
malus_domestica MD02G1161000.v1.1 MD15G1275100.v1.1
prunus_persica Prupe.7G142600_v2.0.a1
pyrus_communis pycom02g12710 pycom15g23820
rosa_chinensis RchiOBHm_Chr2g0103741
rosa_laevigata RLG00000017253
rosa_multiflora Rmu_sc0001852.1_g000002
rosa_roxburghii Rroxscaffold_2G00139440
rosa_rugosa Rorug02G0117000
rosa_samantha Rh2AG165800 Rh2BG173100 Rh2CG171800
rosa_wichuraiana Rw2G012990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1094
AccB7I CCANNNNNTGG 1 cut(s) 904
AciI CCGC 1 cut(s) 943
AclWI GGATC 4 cut(s) 994, 1152, 1163, 1176
AcoI YGGCCR 2 cut(s) 153, 1093
AcsI RAATTY 2 cut(s) 735, 824
AcuI CTGAAG 2 cut(s) 766, 914
AcyI GRCGYC 1 cut(s) 359
AfaI GTAC 2 cut(s) 644, 935
AfiI CCNNNNNNNGG 2 cut(s) 186, 904
AflIII ACRYGT 1 cut(s) 1008
AgsI TTSAA 5 cut(s) 143, 355, 589, 697, 949
AjnI CCWGG 1 cut(s) 982
AjuI GAANNNNNNNTTGG 2 cut(s) 470, 502
AluBI AGCT 7 cut(s) 62, 81, 139, 257, 922, 926, 1190
AluI AGCT 7 cut(s) 62, 81, 139, 257, 922, 926, 1190
Alw26I GTCTC 3 cut(s) 1139, 1221, 1263
AlwI GGATC 4 cut(s) 994, 1152, 1163, 1176
AlwNI CAGNNNCTG 1 cut(s) 1137
AoxI GGCC 4 cut(s) 153, 177, 324, 1093
ApeKI GCWGC 5 cut(s) 81, 84, 233, 515, 1190
ApoI RAATTY 2 cut(s) 735, 824
Asp700I GAANNNNTTC 1 cut(s) 1240
AspS9I GGNCC 4 cut(s) 623, 681, 845, 1121
AsuHPI GGTGA 5 cut(s) 35, 284, 419, 453, 508
AsuNHI GCTAGC 1 cut(s) 922
AvaII GGWCC 4 cut(s) 623, 681, 845, 1121
BalI TGGCCA 2 cut(s) 155, 1095
BamHI GGATCC 1 cut(s) 1168
BanII GRGCYC 1 cut(s) 749
BbvI GCAGC 5 cut(s) 68, 71, 220, 527, 1177
BccI CCATC 5 cut(s) 42, 634, 1018, 1185, 1217
BciT130I CCWGG 1 cut(s) 984
BcoDI GTCTC 3 cut(s) 1139, 1221, 1263
BfaI CTAG 2 cut(s) 798, 923
BfuAI ACCTGC 1 cut(s) 1094
BglII AGATCT 1 cut(s) 656
BisI GCNGC 5 cut(s) 82, 85, 234, 516, 1191
BlsI GCNGC 5 cut(s) 83, 86, 235, 517, 1192
Bme1390I CCNGG 1 cut(s) 984
Bme18I GGWCC 4 cut(s) 623, 681, 845, 1121
BmgT120I GGNCC 4 cut(s) 623, 681, 845, 1121
BmiI GGNNCC 4 cut(s) 336, 624, 682, 1170
BmrFI CCNGG 1 cut(s) 984
BmsI GCATC 8 cut(s) 277, 457, 515, 591, 742, 805, 845, 1143
BmtI GCTAGC 1 cut(s) 926
BpmI CTGGAG 1 cut(s) 384
BsaBI GATNNNNATC 1 cut(s) 605
BsaHI GRCGYC 1 cut(s) 359
BsaI GGTCTC 1 cut(s) 1263
BsaJI CCNNGG 2 cut(s) 156, 1273
Bsc4I CCNNNNNNNGG 2 cut(s) 186, 904
Bse1I ACTGG 4 cut(s) 382, 713, 898, 1131
Bse8I GATNNNNATC 1 cut(s) 605
BseBI CCWGG 1 cut(s) 984
BseDI CCNNGG 2 cut(s) 156, 1273
BseGI GGATG 5 cut(s) 606, 820, 860, 1033, 1177
BseJI GATNNNNATC 1 cut(s) 605
BseLI CCNNNNNNNGG 2 cut(s) 186, 904
BseMII CTCAG 6 cut(s) 206, 228, 755, 959, 1270, 1279
BseNI ACTGG 4 cut(s) 382, 713, 898, 1131
BseXI GCAGC 5 cut(s) 68, 71, 220, 527, 1177
Bsh1285I CGRYCG 1 cut(s) 1151
BshFI GGCC 4 cut(s) 155, 179, 326, 1095
BsiEI CGRYCG 1 cut(s) 1151
BslI CCNNNNNNNGG 2 cut(s) 186, 904
BsmAI GTCTC 3 cut(s) 1139, 1221, 1263
BsmI GAATGC 1 cut(s) 535
BsnI GGCC 4 cut(s) 155, 179, 326, 1095
Bso31I GGTCTC 1 cut(s) 1263
Bsp1286I GDGCHC 1 cut(s) 749
Bsp143I GATC 7 cut(s) 201, 606, 656, 771, 986, 1144, 1168
Bsp19I CCATGG 2 cut(s) 156, 1273
BspACI CCGC 1 cut(s) 943
BspANI GGCC 4 cut(s) 155, 179, 326, 1095
BspCNI CTCAG 6 cut(s) 207, 229, 754, 960, 1269, 1278
BspLI GGNNCC 4 cut(s) 336, 624, 682, 1170
BspMI ACCTGC 1 cut(s) 1094
BspOI GCTAGC 1 cut(s) 926
BspPI GGATC 4 cut(s) 994, 1152, 1163, 1176
BspQI GCTCTTC 1 cut(s) 933
BspTNI GGTCTC 1 cut(s) 1263
BsrI ACTGG 4 cut(s) 382, 713, 898, 1131
BssECI CCNNGG 2 cut(s) 156, 1273
BssMI GATC 7 cut(s) 201, 606, 656, 771, 986, 1144, 1168
BssNI GRCGYC 1 cut(s) 359
BssT1I CCWWGG 2 cut(s) 156, 1273
Bst2UI CCWGG 1 cut(s) 984
Bst4CI ACNGT 2 cut(s) 7, 175
Bst6I CTCTTC 1 cut(s) 933
BstACI GRCGYC 1 cut(s) 359
BstC8I GCNNGC 4 cut(s) 181, 820, 924, 1078
BstDEI CTNAG 7 cut(s) 110, 215, 237, 741, 968, 1256, 1265
BstDSI CCRYGG 2 cut(s) 156, 1273
BstF5I GGATG 5 cut(s) 606, 820, 860, 1033, 1177
BstKTI GATC 7 cut(s) 204, 609, 659, 774, 989, 1147, 1171
BstMAI GTCTC 3 cut(s) 1139, 1221, 1263
BstMBI GATC 7 cut(s) 201, 606, 656, 771, 986, 1144, 1168
BstMCI CGRYCG 1 cut(s) 1151
BstMWI GCNNNNNNNGC 3 cut(s) 512, 919, 1073
BstNI CCWGG 1 cut(s) 984
BstNSI RCATGY 2 cut(s) 1080, 1160
BstSCI CCNGG 1 cut(s) 982
BstV1I GCAGC 5 cut(s) 68, 71, 220, 527, 1177
BstX2I RGATCY 2 cut(s) 656, 1168
BstYI RGATCY 2 cut(s) 656, 1168
BsuRI GGCC 4 cut(s) 155, 179, 326, 1095
BtgI CCRYGG 2 cut(s) 156, 1273
BtsCI GGATG 5 cut(s) 606, 820, 860, 1033, 1177
BtsIMutI CAGTG 2 cut(s) 171, 905
BveI ACCTGC 1 cut(s) 1094
Cac8I GCNNGC 4 cut(s) 181, 820, 924, 1078
CaiI CAGNNNCTG 1 cut(s) 1137
Cfr13I GGNCC 4 cut(s) 623, 681, 845, 1121
CseI GACGC 1 cut(s) 348
Csp6I GTAC 2 cut(s) 643, 934
CviAII CATG 6 cut(s) 151, 157, 1077, 1116, 1157, 1274
CviQI GTAC 2 cut(s) 643, 934
DdeI CTNAG 7 cut(s) 110, 215, 237, 741, 968, 1256, 1265
DpnI GATC 7 cut(s) 203, 608, 658, 773, 988, 1146, 1170
DpnII GATC 7 cut(s) 201, 606, 656, 771, 986, 1144, 1168
EaeI YGGCCR 2 cut(s) 153, 1093
Eam1104I CTCTTC 1 cut(s) 933
EarI CTCTTC 1 cut(s) 933
Eco130I CCWWGG 2 cut(s) 156, 1273
Eco24I GRGCYC 1 cut(s) 749
Eco31I GGTCTC 1 cut(s) 1263
Eco32I GATATC 1 cut(s) 571
Eco47I GGWCC 4 cut(s) 623, 681, 845, 1121
Eco57I CTGAAG 2 cut(s) 766, 914
EcoRII CCWGG 1 cut(s) 982
EcoRV GATATC 1 cut(s) 571
EcoT14I CCWWGG 2 cut(s) 156, 1273
EcoT22I ATGCAT 3 cut(s) 508, 860, 1158
EcoT38I GRGCYC 1 cut(s) 749
ErhI CCWWGG 2 cut(s) 156, 1273
FaeI CATG 6 cut(s) 154, 160, 1080, 1119, 1160, 1277
FatI CATG 6 cut(s) 150, 156, 1076, 1115, 1156, 1273
Fnu4HI GCNGC 5 cut(s) 82, 85, 234, 516, 1191
FokI GGATG 5 cut(s) 613, 827, 867, 1040, 1164
FriOI GRGCYC 1 cut(s) 749
Fsp4HI GCNGC 5 cut(s) 82, 85, 234, 516, 1191
FspBI CTAG 2 cut(s) 798, 923
GluI GCNGC 5 cut(s) 82, 85, 234, 516, 1191
GsuI CTGGAG 1 cut(s) 384
HaeIII GGCC 4 cut(s) 155, 179, 326, 1095
HgaI GACGC 1 cut(s) 348
Hin1I GRCGYC 1 cut(s) 359
Hin1II CATG 6 cut(s) 154, 160, 1080, 1119, 1160, 1277
HindIII AAGCTT 1 cut(s) 137
HinfI GANTC 7 cut(s) 14, 241, 275, 786, 889, 1236, 1252
HphI GGTGA 5 cut(s) 35, 284, 419, 453, 508
Hpy166II GTNNAC 2 cut(s) 387, 1124
Hpy188I TCNGA 6 cut(s) 216, 339, 744, 785, 1259, 1268
Hpy188III TCNNGA 3 cut(s) 363, 510, 893
Hpy8I GTNNAC 2 cut(s) 387, 1124
HpyAV CCTTC 4 cut(s) 316, 375, 943, 989
HpyCH4III ACNGT 2 cut(s) 7, 175
HpyCH4IV ACGT 3 cut(s) 314, 615, 1008
HpyF10VI GCNNNNNNNGC 3 cut(s) 512, 919, 1073
HpyF3I CTNAG 7 cut(s) 110, 215, 237, 741, 968, 1256, 1265
HpySE526I ACGT 3 cut(s) 314, 615, 1008
Hsp92I GRCGYC 1 cut(s) 359
Hsp92II CATG 6 cut(s) 154, 160, 1080, 1119, 1160, 1277
Kzo9I GATC 7 cut(s) 201, 606, 656, 771, 986, 1144, 1168
LguI GCTCTTC 1 cut(s) 933
LmnI GCTCC 3 cut(s) 254, 512, 728
Lsp1109I GCAGC 5 cut(s) 68, 71, 220, 527, 1177
LweI GCATC 8 cut(s) 277, 457, 515, 591, 742, 805, 845, 1143
MaeI CTAG 2 cut(s) 798, 923
MaeII ACGT 3 cut(s) 314, 615, 1008
MaeIII GTNAC 3 cut(s) 95, 459, 670
MalI GATC 7 cut(s) 203, 608, 658, 773, 988, 1146, 1170
MboI GATC 7 cut(s) 201, 606, 656, 771, 986, 1144, 1168
MboII GAAGA 3 cut(s) 434, 552, 920
MfeI CAATTG 2 cut(s) 675, 809
MflI RGATCY 2 cut(s) 656, 1168
MhlI GDGCHC 1 cut(s) 749
MlsI TGGCCA 2 cut(s) 155, 1095
MluNI TGGCCA 2 cut(s) 155, 1095
MlyI GAGTC 1 cut(s) 795
MmeI TCCRAC 3 cut(s) 397, 814, 823
MnlI CCTC 2 cut(s) 359, 728
Mox20I TGGCCA 2 cut(s) 155, 1095
Mph1103I ATGCAT 3 cut(s) 508, 860, 1158
MroXI GAANNNNTTC 1 cut(s) 1240
MscI TGGCCA 2 cut(s) 155, 1095
MseI TTAA 2 cut(s) 210, 552
MslI CAYNNNNRTG 2 cut(s) 155, 1182
Msp20I TGGCCA 2 cut(s) 155, 1095
MspR9I CCNGG 1 cut(s) 984
MunI CAATTG 2 cut(s) 675, 809
Mva1269I GAATGC 1 cut(s) 535
MvaI CCWGG 1 cut(s) 984
MwoI GCNNNNNNNGC 3 cut(s) 512, 919, 1073
NcoI CCATGG 2 cut(s) 156, 1273
NdeII GATC 7 cut(s) 201, 606, 656, 771, 986, 1144, 1168
NheI GCTAGC 1 cut(s) 922
NlaIII CATG 6 cut(s) 154, 160, 1080, 1119, 1160, 1277
NlaIV GGNNCC 4 cut(s) 336, 624, 682, 1170
NmuCI GTSAC 2 cut(s) 459, 670
NsiI ATGCAT 3 cut(s) 508, 860, 1158
NspI RCATGY 2 cut(s) 1080, 1160
PaeI GCATGC 1 cut(s) 1080
PciSI GCTCTTC 1 cut(s) 933
PctI GAATGC 1 cut(s) 535
PdmI GAANNNNTTC 1 cut(s) 1240
PfeI GAWTC 6 cut(s) 14, 241, 275, 889, 1236, 1252
PflMI CCANNNNNTGG 1 cut(s) 904
PfoI TCCNGGA 1 cut(s) 982
PkrI GCNGC 5 cut(s) 83, 86, 235, 517, 1192
PleI GAGTC 1 cut(s) 794
PpsI GAGTC 1 cut(s) 794
Psp6I CCWGG 1 cut(s) 982
PspGI CCWGG 1 cut(s) 982
PspN4I GGNNCC 4 cut(s) 336, 624, 682, 1170
PspPI GGNCC 4 cut(s) 623, 681, 845, 1121
PsrI GAACNNNNNNTAC 2 cut(s) 177, 209
PstNI CAGNNNCTG 1 cut(s) 1137
PsuI RGATCY 2 cut(s) 656, 1168
RsaI GTAC 2 cut(s) 644, 935
RsaNI GTAC 2 cut(s) 643, 934
RseI CAYNNNNRTG 2 cut(s) 155, 1182
SapI GCTCTTC 1 cut(s) 933
SaqAI TTAA 2 cut(s) 210, 552
SatI GCNGC 5 cut(s) 82, 85, 234, 516, 1191
Sau3AI GATC 7 cut(s) 201, 606, 656, 771, 986, 1144, 1168
Sau96I GGNCC 4 cut(s) 623, 681, 845, 1121
SchI GAGTC 1 cut(s) 795
ScrFI CCNGG 1 cut(s) 984
SduI GDGCHC 1 cut(s) 749
SfaNI GCATC 8 cut(s) 277, 457, 515, 591, 742, 805, 845, 1143
SinI GGWCC 4 cut(s) 623, 681, 845, 1121
SmiMI CAYNNNNRTG 2 cut(s) 155, 1182
SphI GCATGC 1 cut(s) 1080
SsiI CCGC 1 cut(s) 943
SspI AATATT 1 cut(s) 546
SspMI CTAG 2 cut(s) 798, 923
StyD4I CCNGG 1 cut(s) 982
StyI CCWWGG 2 cut(s) 156, 1273
TaaI ACNGT 2 cut(s) 7, 175
TaiI ACGT 3 cut(s) 317, 618, 1011
TaqI TCGA 4 cut(s) 126, 320, 609, 1151
TaqII GACCGA 1 cut(s) 1137
TatI WGTACW 2 cut(s) 642, 933
TfiI GAWTC 6 cut(s) 14, 241, 275, 889, 1236, 1252
Tru1I TTAA 2 cut(s) 210, 552
Tru9I TTAA 2 cut(s) 210, 552
TscAI CASTG 2 cut(s) 178, 905
TseFI GTSAC 2 cut(s) 459, 670
TseI GCWGC 5 cut(s) 81, 84, 233, 515, 1190
Tsp45I GTSAC 2 cut(s) 459, 670
TspDTI ATGAA 4 cut(s) 107, 542, 665, 1265
TspRI CASTG 2 cut(s) 178, 905
Van91I CCANNNNNTGG 1 cut(s) 904
VpaK11BI GGWCC 4 cut(s) 623, 681, 845, 1121
XapI RAATTY 2 cut(s) 735, 824
XceI RCATGY 2 cut(s) 1080, 1160
XmnI GAANNNNTTC 1 cut(s) 1240
XspI CTAG 2 cut(s) 798, 923
Zsp2I ATGCAT 3 cut(s) 508, 860, 1158
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.