pycom02g14110

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0010165
Physical Location & Seq
Forward (+)
10702748 .. 10704904
2157 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g14110.1

Sequence Viewer

Length: 681 bp
ATGGAGGGAGAGGCGGAGATTGATCTGTTGCCAATAGACCTCTTGGCTCATATATTTGTTCTCATCACCTCCTTCACTGATTTAGCCCAGGCAAGCGGTGTTTGCCGGAAATGGAAACAGGCGGTGAAGCAGTCTTTGGGGCGGAGGGAGAGTTTGAGCTTTGCTGGTTGGAAGATGGACGATGACTCCACCGCCCGTTTGCTCCGCTCTGCTTACAGCCTCAAAGAGCTCGACATTTCAAGGCGTCGCTGGGGTTGCCAGATAACCGACACTGGATTGTACAGAATATCTATGGCAAAGTGTATCAGCAACTTAACATCCATATCGCTATGGGGCATCACAGGGATCACAGATAAAGGTGTTGTTCAACTGATATCAAGAGCTAATTCCTTGCACCACCTGAATATCGGTGGCACATTTATCACAGATGACTCGTTATGTGCCATTGCTCATAGCTGTCCGCATTTGAAGACTATCATCCTGTGGAGCTGCCGTCATGTAACCGAGAACGGCCTCTTTGTTCTAGTAAACTGTTGCCGCAAGCTTGAATCCATCAATGTATGGGGGACGAGAGTTCCTGTAGACTGCTTCATTGCTTTGCTTACTATCAGTCCGGCACTAAAGATAAAACCCAGAGGACTGTCGTTAAATGTGGACGCTTCGATGTTGCCCATCGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box-like LRR_6

Protein Analysis

227

Amino Acids

24.98

Weight (kDa)

8.76

Isoelectric Point (pI)

41.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 7 - 44 1.1e-07 F-box-like
DUF7885 PF25372 95 - 176 1e-08 Leucine Rich Repeat Domain of unknown function (DUF7885)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 207
AccI GTMKAC 1 cut(s) 582
AciI CCGC 8 cut(s) 14, 96, 122, 142, 192, 205, 461, 538
AclWI GGATC 1 cut(s) 353
AcyI GRCGYC 1 cut(s) 244
AfaI GTAC 1 cut(s) 281
AgsI TTSAA 4 cut(s) 240, 368, 469, 548
AjnI CCWGG 1 cut(s) 87
AjuI GAANNNNNNNTTGG 2 cut(s) 119, 151
AluBI AGCT 6 cut(s) 159, 229, 383, 456, 489, 544
AluI AGCT 6 cut(s) 159, 229, 383, 456, 489, 544
Alw21I GWGCWC 1 cut(s) 231
AlwI GGATC 1 cut(s) 353
AoxI GGCC 1 cut(s) 511
ApeKI GCWGC 1 cut(s) 489
AsuHPI GGTGA 2 cut(s) 58, 136
BanII GRGCYC 1 cut(s) 231
BbsI GAAGAC 1 cut(s) 476
Bbv12I GWGCWC 1 cut(s) 231
BbvI GCAGC 1 cut(s) 476
BccI CCATC 3 cut(s) 169, 560, 680
BceAI ACGGC 2 cut(s) 477, 526
BciT130I CCWGG 1 cut(s) 89
BfaI CTAG 1 cut(s) 524
BfmI CTRYAG 1 cut(s) 579
BisI GCNGC 2 cut(s) 490, 538
BlsI GCNGC 2 cut(s) 491, 539
Bme1390I CCNGG 1 cut(s) 89
BmrFI CCNGG 1 cut(s) 89
BmsI GCATC 1 cut(s) 345
BpiI GAAGAC 1 cut(s) 476
BsaHI GRCGYC 1 cut(s) 244
BsaJI CCNNGG 1 cut(s) 87
BsaXI ACNNNNNCTCC 6 cut(s) 136, 166, 170, 200, 478, 508
Bse1I ACTGG 1 cut(s) 277
Bse3DI GCAATG 2 cut(s) 444, 591
BseBI CCWGG 1 cut(s) 89
BseDI CCNNGG 1 cut(s) 87
BseGI GGATG 2 cut(s) 317, 477
BseMI GCAATG 2 cut(s) 444, 591
BseNI ACTGG 1 cut(s) 277
BseXI GCAGC 1 cut(s) 476
BseYI CCCAGC 1 cut(s) 249
BshFI GGCC 1 cut(s) 513
BsiHKAI GWGCWC 1 cut(s) 231
BsiSI CCGG 2 cut(s) 106, 614
BslFI GGGAC 1 cut(s) 580
BsmFI GGGAC 1 cut(s) 580
BsnI GGCC 1 cut(s) 513
Bsp1286I GDGCHC 1 cut(s) 231
Bsp1407I TGTACA 1 cut(s) 279
Bsp143I GATC 2 cut(s) 22, 345
BspACI CCGC 8 cut(s) 14, 96, 122, 142, 192, 205, 461, 538
BspANI GGCC 1 cut(s) 513
BspPI GGATC 1 cut(s) 353
BsrBI CCGCTC 1 cut(s) 207
BsrDI GCAATG 2 cut(s) 444, 591
BsrGI TGTACA 1 cut(s) 279
BsrI ACTGG 1 cut(s) 277
BssECI CCNNGG 1 cut(s) 87
BssMI GATC 2 cut(s) 22, 345
BssNI GRCGYC 1 cut(s) 244
Bst2UI CCWGG 1 cut(s) 89
Bst4CI ACNGT 2 cut(s) 533, 642
BstACI GRCGYC 1 cut(s) 244
BstAUI TGTACA 1 cut(s) 279
BstC8I GCNNGC 2 cut(s) 94, 542
BstF5I GGATG 2 cut(s) 317, 477
BstKTI GATC 2 cut(s) 25, 348
BstMBI GATC 2 cut(s) 22, 345
BstMWI GCNNNNNNNGC 2 cut(s) 102, 255
BstNI CCWGG 1 cut(s) 89
BstSCI CCNGG 1 cut(s) 87
BstSFI CTRYAG 1 cut(s) 579
BstV1I GCAGC 1 cut(s) 476
BstV2I GAAGAC 1 cut(s) 476
BsuRI GGCC 1 cut(s) 513
BtgZI GCGATG 1 cut(s) 658
BtsCI GGATG 2 cut(s) 317, 477
BtsIMutI CAGTG 2 cut(s) 75, 270
Cac8I GCNNGC 2 cut(s) 94, 542
CseI GACGC 2 cut(s) 233, 665
Csp6I GTAC 1 cut(s) 280
CviAII CATG 1 cut(s) 497
CviQI GTAC 1 cut(s) 280
DpnI GATC 2 cut(s) 24, 347
DpnII GATC 2 cut(s) 22, 345
EciI GGCGGA 2 cut(s) 29, 157
Ecl136II GAGCTC 1 cut(s) 229
Eco24I GRGCYC 1 cut(s) 231
Eco32I GATATC 1 cut(s) 375
Eco53kI GAGCTC 1 cut(s) 229
EcoICRI GAGCTC 1 cut(s) 229
EcoRII CCWGG 1 cut(s) 87
EcoRV GATATC 1 cut(s) 375
EcoT38I GRGCYC 1 cut(s) 231
FaeI CATG 1 cut(s) 500
FaqI GGGAC 1 cut(s) 580
FatI CATG 1 cut(s) 496
FblI GTMKAC 1 cut(s) 582
Fnu4HI GCNGC 2 cut(s) 490, 538
FokI GGATG 2 cut(s) 304, 464
FriOI GRGCYC 1 cut(s) 231
Fsp4HI GCNGC 2 cut(s) 490, 538
FspBI CTAG 1 cut(s) 524
GluI GCNGC 2 cut(s) 490, 538
GsaI CCCAGC 1 cut(s) 253
HaeIII GGCC 1 cut(s) 513
HapII CCGG 2 cut(s) 106, 614
HgaI GACGC 2 cut(s) 233, 665
Hin1I GRCGYC 1 cut(s) 244
Hin1II CATG 1 cut(s) 500
HindIII AAGCTT 1 cut(s) 542
HinfI GANTC 3 cut(s) 185, 431, 548
HpaII CCGG 2 cut(s) 106, 614
HphI GGTGA 2 cut(s) 58, 136
Hpy166II GTNNAC 3 cut(s) 529, 583, 655
Hpy188III TCNNGA 1 cut(s) 378
Hpy8I GTNNAC 3 cut(s) 529, 583, 655
Hpy99I CGWCG 1 cut(s) 249
HpyAV CCTTC 1 cut(s) 82
HpyCH4III ACNGT 2 cut(s) 533, 642
HpyCH4V TGCA 1 cut(s) 394
HpyF10VI GCNNNNNNNGC 2 cut(s) 102, 255
Hsp92I GRCGYC 1 cut(s) 244
Hsp92II CATG 1 cut(s) 500
Kzo9I GATC 2 cut(s) 22, 345
LmnI GCTCC 2 cut(s) 207, 486
Lsp1109I GCAGC 1 cut(s) 476
LweI GCATC 1 cut(s) 345
MaeI CTAG 1 cut(s) 524
MaeIII GTNAC 1 cut(s) 499
MalI GATC 2 cut(s) 24, 347
MbiI CCGCTC 1 cut(s) 207
MboI GATC 2 cut(s) 22, 345
MboII GAAGA 2 cut(s) 184, 481
MhlI GDGCHC 1 cut(s) 231
MluCI AATT 1 cut(s) 385
MlyI GAGTC 2 cut(s) 179, 425
MmeI TCCRAC 1 cut(s) 149
MnlI CCTC 7 cut(s) 4, 50, 79, 138, 230, 524, 629
MseI TTAA 2 cut(s) 314, 647
MspI CCGG 2 cut(s) 106, 614
MspR9I CCNGG 1 cut(s) 89
MvaI CCWGG 1 cut(s) 89
MwoI GCNNNNNNNGC 2 cut(s) 102, 255
NdeII GATC 2 cut(s) 22, 345
NlaIII CATG 1 cut(s) 500
PfeI GAWTC 1 cut(s) 548
PkrI GCNGC 2 cut(s) 491, 539
PleI GAGTC 2 cut(s) 179, 425
PpsI GAGTC 2 cut(s) 179, 425
Psp124BI GAGCTC 1 cut(s) 231
Psp6I CCWGG 1 cut(s) 87
PspFI CCCAGC 1 cut(s) 249
PspGI CCWGG 1 cut(s) 87
RsaI GTAC 1 cut(s) 281
RsaNI GTAC 1 cut(s) 280
SacI GAGCTC 1 cut(s) 231
SaqAI TTAA 2 cut(s) 314, 647
SatI GCNGC 2 cut(s) 490, 538
Sau3AI GATC 2 cut(s) 22, 345
SchI GAGTC 2 cut(s) 179, 425
ScrFI CCNGG 1 cut(s) 89
SduI GDGCHC 1 cut(s) 231
SfaNI GCATC 1 cut(s) 345
SfcI CTRYAG 1 cut(s) 579
Sse9I AATT 1 cut(s) 385
SsiI CCGC 8 cut(s) 14, 96, 122, 142, 192, 205, 461, 538
SspMI CTAG 1 cut(s) 524
SstI GAGCTC 1 cut(s) 231
StyD4I CCNGG 1 cut(s) 87
TaaI ACNGT 2 cut(s) 533, 642
TaqI TCGA 2 cut(s) 231, 662
TasI AATT 1 cut(s) 385
TatI WGTACW 1 cut(s) 279
TauI GCSGC 1 cut(s) 540
TfiI GAWTC 1 cut(s) 548
Tru1I TTAA 2 cut(s) 314, 647
Tru9I TTAA 2 cut(s) 314, 647
TscAI CASTG 2 cut(s) 82, 277
TseI GCWGC 1 cut(s) 489
TspDTI ATGAA 1 cut(s) 580
TspRI CASTG 2 cut(s) 82, 277
XmiI GTMKAC 1 cut(s) 582
XspI CTAG 1 cut(s) 524
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.