Rw2G014340

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0010165
Physical Location & Seq
Forward (+)
16060802 .. 16062898
2097 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G014340.1

Sequence Viewer

Length: 546 bp
ATGGAGGGAGAGGCTGAGATTGATCGGTTGCCTCTAGACCTCTTGGCTAATATATTTGTTCTCATCACTTCCTTCACTGATTTGGCACAAGCAAGCGGTGTGTGTAGAAAATGGAAACAGGGGGTGAAGCAGAGTCTGGGACGCAGAGACAGTTTGAGCTTTGCTGGTTGGAAGATGGATGATGACTCCACAGCTCGTCTGCTTCGCTATGCTTATAGCCTCATAGACTTGGATATTTCAAGGAGCCGGTGGGGTTGTCAGATAACCGACACCGGATTGTACCGGATCTCTTTGGCAAAGTGTATCAGCAATTTAACATCCATATCGTTATGGGGTGTTACAGGGATCACGGATAAAGGTGTTGTTCATTTGATATCCAGAGCTAATTCCCTGCAGCACCTGAATATTGGTGGCACATTCATCACAGATGAATCACTGTATGTCATTGCTAATAGCTGTCCACATTTGAAGGTATGCTGTTCAAGGCGAATTAGGGCATGTTTTGAAGTGCTTTTGTTAAAAGCGCTTTTACTAGGTAGTACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box-like LRR_6

Protein Analysis

181

Amino Acids

20.04

Weight (kDa)

8.72

Isoelectric Point (pI)

46.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 7 - 40 6.1e-07 F-box-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 96
AclWI GGATC 2 cut(s) 293, 353
AfaI GTAC 2 cut(s) 281, 541
AfeI AGCGCT 1 cut(s) 525
AgsI TTSAA 4 cut(s) 240, 469, 483, 506
AluBI AGCT 4 cut(s) 159, 194, 383, 456
AluI AGCT 4 cut(s) 159, 194, 383, 456
Alw26I GTCTC 1 cut(s) 141
AlwI GGATC 2 cut(s) 293, 353
AlwNI CAGNNNCTG 2 cut(s) 136, 400
Aor51HI AGCGCT 1 cut(s) 525
ApeKI GCWGC 1 cut(s) 394
AspLEI GCGC 1 cut(s) 526
AsuHPI GGTGA 1 cut(s) 136
BbvI GCAGC 1 cut(s) 406
BccI CCATC 1 cut(s) 169
BcoDI GTCTC 1 cut(s) 141
BfaI CTAG 2 cut(s) 35, 533
BfmI CTRYAG 1 cut(s) 392
BfoI RGCGCY 1 cut(s) 527
BisI GCNGC 1 cut(s) 395
BlsI GCNGC 1 cut(s) 396
BmcAI AGTACT 1 cut(s) 541
BmiI GGNNCC 1 cut(s) 245
BsaWI WCCGGW 2 cut(s) 272, 282
Bse118I RCCGGY 1 cut(s) 246
Bse3DI GCAATG 1 cut(s) 444
BseGI GGATG 2 cut(s) 184, 317
BseMI GCAATG 1 cut(s) 444
BseMII CTCAG 1 cut(s) 6
BseXI GCAGC 1 cut(s) 406
BsiSI CCGG 3 cut(s) 247, 273, 283
BslFI GGGAC 1 cut(s) 153
BsmAI GTCTC 1 cut(s) 141
BsmFI GGGAC 1 cut(s) 153
Bsp143I GATC 3 cut(s) 22, 285, 345
BspACI CCGC 1 cut(s) 96
BspCNI CTCAG 1 cut(s) 7
BspLI GGNNCC 1 cut(s) 245
BspMAI CTGCAG 1 cut(s) 396
BspPI GGATC 2 cut(s) 293, 353
BsrDI GCAATG 1 cut(s) 444
BsrFI RCCGGY 1 cut(s) 246
BssAI RCCGGY 1 cut(s) 246
BssMI GATC 3 cut(s) 22, 285, 345
Bst4CI ACNGT 2 cut(s) 152, 438
BstC8I GCNNGC 1 cut(s) 94
BstDEI CTNAG 1 cut(s) 15
BstF5I GGATG 2 cut(s) 184, 317
BstH2I RGCGCY 1 cut(s) 527
BstHHI GCGC 1 cut(s) 526
BstKTI GATC 3 cut(s) 25, 288, 348
BstMAI GTCTC 1 cut(s) 141
BstMBI GATC 3 cut(s) 22, 285, 345
BstNSI RCATGY 1 cut(s) 501
BstSFI CTRYAG 1 cut(s) 392
BstV1I GCAGC 1 cut(s) 406
BstX2I RGATCY 1 cut(s) 285
BstYI RGATCY 1 cut(s) 285
BtsCI GGATG 2 cut(s) 184, 317
BtsIMutI CAGTG 2 cut(s) 75, 434
Cac8I GCNNGC 1 cut(s) 94
CaiI CAGNNNCTG 2 cut(s) 136, 400
CfoI GCGC 1 cut(s) 526
Cfr10I RCCGGY 1 cut(s) 246
CseI GACGC 1 cut(s) 150
Csp6I GTAC 2 cut(s) 280, 540
CviAII CATG 1 cut(s) 498
CviJI RGCY 8 cut(s) 14, 47, 159, 194, 219, 246, 383, 456
CviKI_1 RGCY 8 cut(s) 14, 47, 159, 194, 219, 246, 383, 456
CviQI GTAC 2 cut(s) 280, 540
DdeI CTNAG 1 cut(s) 15
DpnI GATC 3 cut(s) 24, 287, 347
DpnII GATC 3 cut(s) 22, 285, 345
Eco32I GATATC 1 cut(s) 375
Eco47III AGCGCT 1 cut(s) 525
EcoRV GATATC 1 cut(s) 375
FaeI CATG 1 cut(s) 501
FaiI YATR 9 cut(s) 53, 210, 216, 224, 323, 331, 441, 475, 499
FaqI GGGAC 1 cut(s) 153
FatI CATG 1 cut(s) 497
Fnu4HI GCNGC 1 cut(s) 395
FokI GGATG 2 cut(s) 191, 304
Fsp4HI GCNGC 1 cut(s) 395
FspBI CTAG 2 cut(s) 35, 533
GlaI GCGC 1 cut(s) 525
GluI GCNGC 1 cut(s) 395
HaeII RGCGCY 1 cut(s) 527
HapII CCGG 3 cut(s) 247, 273, 283
HgaI GACGC 1 cut(s) 150
HhaI GCGC 1 cut(s) 526
Hin1II CATG 1 cut(s) 501
Hin6I GCGC 1 cut(s) 524
HinP1I GCGC 1 cut(s) 524
HinfI GANTC 3 cut(s) 133, 185, 431
HpaII CCGG 3 cut(s) 247, 273, 283
HphI GGTGA 1 cut(s) 136
Hpy166II GTNNAC 1 cut(s) 461
Hpy188I TCNGA 1 cut(s) 261
Hpy188III TCNNGA 2 cut(s) 35, 378
Hpy8I GTNNAC 1 cut(s) 461
HpyAV CCTTC 2 cut(s) 82, 463
HpyCH4III ACNGT 2 cut(s) 152, 438
HpyCH4V TGCA 1 cut(s) 394
HpyF3I CTNAG 1 cut(s) 15
Hsp92II CATG 1 cut(s) 501
HspAI GCGC 1 cut(s) 524
Kzo9I GATC 3 cut(s) 22, 285, 345
LmnI GCTCC 1 cut(s) 243
Lsp1109I GCAGC 1 cut(s) 406
MaeI CTAG 2 cut(s) 35, 533
MaeIII GTNAC 1 cut(s) 337
MalI GATC 3 cut(s) 24, 287, 347
MboI GATC 3 cut(s) 22, 285, 345
MboII GAAGA 1 cut(s) 184
MflI RGATCY 1 cut(s) 285
MluCI AATT 3 cut(s) 310, 385, 489
MlyI GAGTC 2 cut(s) 142, 179
MmeI TCCRAC 1 cut(s) 149
MnlI CCTC 4 cut(s) 4, 42, 50, 230
MseI TTAA 3 cut(s) 314, 518, 544
MspI CCGG 3 cut(s) 247, 273, 283
NdeII GATC 3 cut(s) 22, 285, 345
NlaIII CATG 1 cut(s) 501
NlaIV GGNNCC 1 cut(s) 245
NspI RCATGY 1 cut(s) 501
PfeI GAWTC 1 cut(s) 431
PkrI GCNGC 1 cut(s) 396
PleI GAGTC 2 cut(s) 141, 179
PpsI GAGTC 2 cut(s) 141, 179
PspN4I GGNNCC 1 cut(s) 245
PstI CTGCAG 1 cut(s) 396
PstNI CAGNNNCTG 2 cut(s) 136, 400
PsuI RGATCY 1 cut(s) 285
RsaI GTAC 2 cut(s) 281, 541
RsaNI GTAC 2 cut(s) 280, 540
SaqAI TTAA 3 cut(s) 314, 518, 544
SatI GCNGC 1 cut(s) 395
Sau3AI GATC 3 cut(s) 22, 285, 345
ScaI AGTACT 1 cut(s) 541
SchI GAGTC 2 cut(s) 142, 179
SetI ASST 9 cut(s) 42, 161, 196, 361, 385, 402, 458, 474, 538
SfcI CTRYAG 1 cut(s) 392
Sse9I AATT 3 cut(s) 310, 385, 489
SsiI CCGC 1 cut(s) 96
SspI AATATT 1 cut(s) 406
SspMI CTAG 2 cut(s) 35, 533
TaaI ACNGT 2 cut(s) 152, 438
TasI AATT 3 cut(s) 310, 385, 489
TatI WGTACW 1 cut(s) 539
TfiI GAWTC 1 cut(s) 431
Tru1I TTAA 3 cut(s) 314, 518, 544
Tru9I TTAA 3 cut(s) 314, 518, 544
TscAI CASTG 2 cut(s) 82, 441
TseI GCWGC 1 cut(s) 394
TspDTI ATGAA 3 cut(s) 356, 409, 444
TspGWI ACGGA 1 cut(s) 365
TspRI CASTG 2 cut(s) 82, 441
XbaI TCTAGA 1 cut(s) 34
XceI RCATGY 1 cut(s) 501
XspI CTAG 2 cut(s) 35, 533
ZrmI AGTACT 1 cut(s) 541
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.