pycom02g17040

Involved in transport from the ER to the Golgi apparatus as well as in intra-Golgi transport. It belongs to a super-family of proteins called t-SNAREs or soluble NSF (N-ethylmaleimide- sensitive factor) attachment protein receptor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0011092
Physical Location & Seq
Forward (+)
14510853 .. 14515561
4709 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 717 bp
ATGACGGATTCGAATCTGGAGTTGCAGGAATCGGGTTGGGAGGAGCTGAGGAGAGAGGCCAGGAAGATTGAGGGAGATCTTGATGTGAATCTCTCTTCTTACGCTAAGCTTGGTGCTAGGTTCACACAAGGTTATGTGGAATCTGGGTCACCATCTGTTAGTTCTAGCAGGTCATGGAAGTCCATGGAAATGGAGATCCAGTCGTTGCTTGAGAAACTTTTGGAAACAAATGATTCAATGAGTCGATGTGCTGCATCTGCTACACCAGCTACTTCTGTAACTCAGAAGCTAGCAAGGCACAGAGACATACTTCATGAGTTTACCCAGGAGTTTAGAAGAATCAAGGGGAACATAAATTCATTGAGGGAACATGCGGAGCTTCTGACTTCAGTCAGGGACGATATCAGTGAGTACAAAGCATCTGGGATTATGTCACCAAGAATGCAGATACTACGGGAAAGAGCTGCCATCCATGGAAGTGTATCGCATATAGATGAGGTGATTAGTCAAGCTCAAACAACACGAGCAGTCTTGGGCTCTCAAAGGGCTCTGTTTGGAGATGTTCAAGGGAAAGTGAAAAATCTAAGTGACAAGTTCCCTATAGTCCGTGGCCTACTCGGTTCAATTCGAAGGCGGCGATCAAGAGACACACTTATTTTGTCTGCAGTCATTGCAGCTTGTAGCTTGTTTCTTATTATCTACTGGCTTTCAAAATAA

Protein Analysis

239

Amino Acids

26.71

Weight (kDa)

9.32

Isoelectric Point (pI)

58.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
V-SNARE_C PF12352 149 - 213 4.9e-16 Snare region anchored in the vesicle membrane C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011092)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G45200 AT2G45200
fragaria_vesca FvH4_7g11270
malus_domestica MD02G1205800.v1.1 MD07G1117300.v1.1
prunus_persica Prupe.2G140500_v2.0.a1 Prupe.2G140500_v2.0.a1
pyrus_communis pycom02g17040 pycom07g10740
rosa_chinensis RchiOBHm_Chr1g0349411
rosa_laevigata RLG00000028551
rosa_multiflora Rmu_sc0002495.1_g000004
rosa_roxburghii Rroxscaffold_4G00305560
rosa_rugosa Rorug01G0203500 Rorug01G0203600.1
rosa_samantha Rh1AG220900 Rh1BG187000 Rh1CG205900 Rh1DG216300
rosa_wichuraiana Rw1G018770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 159
AciI CCGC 2 cut(s) 374, 634
AclWI GGATC 1 cut(s) 190
AcsI RAATTY 1 cut(s) 355
AcuI CTGAAG 1 cut(s) 372
AfaI GTAC 1 cut(s) 413
AgsI TTSAA 4 cut(s) 237, 566, 624, 711
AjnI CCWGG 2 cut(s) 59, 324
AluBI AGCT 9 cut(s) 46, 109, 269, 289, 379, 464, 512, 677, 684
AluI AGCT 9 cut(s) 46, 109, 269, 289, 379, 464, 512, 677, 684
Alw26I GTCTC 2 cut(s) 297, 639
AlwI GGATC 1 cut(s) 190
AoxI GGCC 2 cut(s) 57, 610
ApeKI GCWGC 3 cut(s) 251, 464, 674
ApoI RAATTY 1 cut(s) 355
AsuHPI GGTGA 3 cut(s) 141, 426, 511
AsuII TTCGAA 2 cut(s) 11, 628
AsuNHI GCTAGC 1 cut(s) 289
BanII GRGCYC 2 cut(s) 539, 550
BauI CACGAG 1 cut(s) 522
BbvCI CCTCAGC 1 cut(s) 47
BbvI GCAGC 3 cut(s) 238, 451, 686
BccI CCATC 2 cut(s) 160, 476
BciT130I CCWGG 2 cut(s) 61, 326
BcoDI GTCTC 2 cut(s) 297, 639
BfaI CTAG 3 cut(s) 117, 165, 290
BfmI CTRYAG 2 cut(s) 600, 663
BfuAI ACCTGC 1 cut(s) 159
BglII AGATCT 1 cut(s) 76
BisI GCNGC 4 cut(s) 252, 465, 635, 675
BlpI GCTNAGC 1 cut(s) 105
BlsI GCNGC 4 cut(s) 253, 466, 636, 676
Bme1390I CCNGG 2 cut(s) 61, 326
BmrFI CCNGG 2 cut(s) 61, 326
BmsI GCATC 2 cut(s) 263, 428
BmtI GCTAGC 1 cut(s) 293
BoxI GACNNNNGTC 1 cut(s) 389
BpmI CTGGAG 1 cut(s) 38
Bpu10I CCTNAGC 1 cut(s) 47
Bpu1102I GCTNAGC 1 cut(s) 105
Bpu14I TTCGAA 2 cut(s) 11, 628
BpuEI CTTGAG 1 cut(s) 230
BsaBI GATNNNNATC 2 cut(s) 12, 87
BsaJI CCNNGG 4 cut(s) 183, 324, 472, 607
BsaXI ACNNNNNCTCC 2 cut(s) 185, 215
Bse1I ACTGG 2 cut(s) 199, 707
Bse3DI GCAATG 1 cut(s) 669
Bse8I GATNNNNATC 2 cut(s) 12, 87
BseBI CCWGG 2 cut(s) 61, 326
BseDI CCNNGG 4 cut(s) 183, 324, 472, 607
BseGI GGATG 1 cut(s) 468
BseJI GATNNNNATC 2 cut(s) 12, 87
BseMI GCAATG 1 cut(s) 669
BseMII CTCAG 2 cut(s) 38, 296
BseNI ACTGG 2 cut(s) 199, 707
BseRI GAGGAG 2 cut(s) 56, 64
BseXI GCAGC 3 cut(s) 238, 451, 686
BshFI GGCC 2 cut(s) 59, 612
BslFI GGGAC 1 cut(s) 410
BsmAI GTCTC 2 cut(s) 297, 639
BsmFI GGGAC 1 cut(s) 410
BsmI GAATGC 1 cut(s) 447
BsnI GGCC 2 cut(s) 59, 612
Bsp119I TTCGAA 2 cut(s) 11, 628
Bsp1286I GDGCHC 2 cut(s) 539, 550
Bsp143I GATC 3 cut(s) 76, 195, 638
Bsp1720I GCTNAGC 1 cut(s) 105
Bsp19I CCATGG 2 cut(s) 183, 472
BspACI CCGC 2 cut(s) 374, 634
BspANI GGCC 2 cut(s) 59, 612
BspCNI CTCAG 2 cut(s) 39, 295
BspHI TCATGA 1 cut(s) 313
BspMAI CTGCAG 1 cut(s) 667
BspMI ACCTGC 1 cut(s) 159
BspOI GCTAGC 1 cut(s) 293
BspPI GGATC 1 cut(s) 190
BspT104I TTCGAA 2 cut(s) 11, 628
BsrDI GCAATG 1 cut(s) 669
BsrI ACTGG 2 cut(s) 199, 707
BssECI CCNNGG 4 cut(s) 183, 324, 472, 607
BssMI GATC 3 cut(s) 76, 195, 638
BssSI CACGAG 1 cut(s) 522
BssT1I CCWWGG 2 cut(s) 183, 472
Bst2BI CACGAG 1 cut(s) 522
Bst2UI CCWGG 2 cut(s) 61, 326
Bst6I CTCTTC 1 cut(s) 100
BstAPI GCANNNNNTGC 1 cut(s) 671
BstBI TTCGAA 2 cut(s) 11, 628
BstC8I GCNNGC 1 cut(s) 291
BstDEI CTNAG 4 cut(s) 47, 105, 282, 584
BstDSI CCRYGG 3 cut(s) 183, 472, 607
BstEII GGTNACC 1 cut(s) 147
BstF5I GGATG 1 cut(s) 468
BstKTI GATC 3 cut(s) 79, 198, 641
BstMAI GTCTC 2 cut(s) 297, 639
BstMBI GATC 3 cut(s) 76, 195, 638
BstMWI GCNNNNNNNGC 4 cut(s) 257, 266, 295, 671
BstNI CCWGG 2 cut(s) 61, 326
BstNSI RCATGY 1 cut(s) 374
BstPAI GACNNNNGTC 1 cut(s) 389
BstPI GGTNACC 1 cut(s) 147
BstSCI CCNGG 2 cut(s) 59, 324
BstSFI CTRYAG 2 cut(s) 600, 663
BstV1I GCAGC 3 cut(s) 238, 451, 686
BstX2I RGATCY 2 cut(s) 76, 195
BstXI CCANNNNNNTGG 1 cut(s) 190
BstYI RGATCY 2 cut(s) 76, 195
BsuRI GGCC 2 cut(s) 59, 612
BtgI CCRYGG 3 cut(s) 183, 472, 607
BtsCI GGATG 1 cut(s) 468
BtsIMutI CAGTG 1 cut(s) 412
BveI ACCTGC 1 cut(s) 159
Cac8I GCNNGC 1 cut(s) 291
CciI TCATGA 1 cut(s) 313
Csp6I GTAC 1 cut(s) 412
CviAII CATG 5 cut(s) 174, 184, 314, 371, 473
CviQI GTAC 1 cut(s) 412
DdeI CTNAG 4 cut(s) 47, 105, 282, 584
DpnI GATC 3 cut(s) 78, 197, 640
DpnII GATC 3 cut(s) 76, 195, 638
Eam1104I CTCTTC 1 cut(s) 100
EarI CTCTTC 1 cut(s) 100
Eco130I CCWWGG 2 cut(s) 183, 472
Eco24I GRGCYC 2 cut(s) 539, 550
Eco32I GATATC 1 cut(s) 403
Eco57I CTGAAG 1 cut(s) 372
Eco91I GGTNACC 1 cut(s) 147
EcoO65I GGTNACC 1 cut(s) 147
EcoRII CCWGG 2 cut(s) 59, 324
EcoRV GATATC 1 cut(s) 403
EcoT14I CCWWGG 2 cut(s) 183, 472
EcoT38I GRGCYC 2 cut(s) 539, 550
ErhI CCWWGG 2 cut(s) 183, 472
FaeI CATG 5 cut(s) 177, 187, 317, 374, 476
FaqI GGGAC 1 cut(s) 410
FatI CATG 5 cut(s) 173, 183, 313, 370, 472
Fnu4HI GCNGC 4 cut(s) 252, 465, 635, 675
FokI GGATG 1 cut(s) 455
FriOI GRGCYC 2 cut(s) 539, 550
Fsp4HI GCNGC 4 cut(s) 252, 465, 635, 675
FspBI CTAG 3 cut(s) 117, 165, 290
GluI GCNGC 4 cut(s) 252, 465, 635, 675
GsuI CTGGAG 1 cut(s) 38
HaeIII GGCC 2 cut(s) 59, 612
Hin1II CATG 5 cut(s) 177, 187, 317, 374, 476
HindIII AAGCTT 1 cut(s) 107
HinfI GANTC 8 cut(s) 8, 13, 29, 88, 140, 233, 241, 339
HphI GGTGA 3 cut(s) 141, 426, 511
Hpy166II GTNNAC 2 cut(s) 123, 321
Hpy188I TCNGA 2 cut(s) 285, 384
Hpy188III TCNNGA 4 cut(s) 17, 80, 314, 642
Hpy8I GTNNAC 2 cut(s) 123, 321
HpyAV CCTTC 1 cut(s) 624
HpyCH4V TGCA 5 cut(s) 25, 254, 445, 665, 674
HpyF10VI GCNNNNNNNGC 4 cut(s) 257, 266, 295, 671
HpyF3I CTNAG 4 cut(s) 47, 105, 282, 584
Hsp92II CATG 5 cut(s) 177, 187, 317, 374, 476
Kzo9I GATC 3 cut(s) 76, 195, 638
LmnI GCTCC 2 cut(s) 43, 376
Lsp1109I GCAGC 3 cut(s) 238, 451, 686
LweI GCATC 2 cut(s) 263, 428
MaeI CTAG 3 cut(s) 117, 165, 290
MaeIII GTNAC 4 cut(s) 147, 277, 432, 587
MalI GATC 3 cut(s) 78, 197, 640
MboI GATC 3 cut(s) 76, 195, 638
MboII GAAGA 3 cut(s) 76, 87, 348
MflI RGATCY 2 cut(s) 76, 195
MhlI GDGCHC 2 cut(s) 539, 550
MluCI AATT 2 cut(s) 355, 624
MlyI GAGTC 1 cut(s) 250
MnlI CCTC 6 cut(s) 34, 42, 49, 64, 357, 490
MslI CAYNNNNRTG 3 cut(s) 188, 477, 492
MspR9I CCNGG 2 cut(s) 61, 326
Mva1269I GAATGC 1 cut(s) 447
MvaI CCWGG 2 cut(s) 61, 326
MwoI GCNNNNNNNGC 4 cut(s) 257, 266, 295, 671
NcoI CCATGG 2 cut(s) 183, 472
NdeII GATC 3 cut(s) 76, 195, 638
NheI GCTAGC 1 cut(s) 289
NlaIII CATG 5 cut(s) 177, 187, 317, 374, 476
NmuCI GTSAC 3 cut(s) 147, 432, 587
NspI RCATGY 1 cut(s) 374
NspV TTCGAA 2 cut(s) 11, 628
PagI TCATGA 1 cut(s) 313
PcsI WCGNNNNNNNCGW 1 cut(s) 634
PctI GAATGC 1 cut(s) 447
PfeI GAWTC 7 cut(s) 8, 13, 29, 88, 140, 233, 339
PkrI GCNGC 4 cut(s) 253, 466, 636, 676
PleI GAGTC 1 cut(s) 249
PpsI GAGTC 1 cut(s) 249
PshAI GACNNNNGTC 1 cut(s) 389
Psp6I CCWGG 2 cut(s) 59, 324
PspEI GGTNACC 1 cut(s) 147
PspGI CCWGG 2 cut(s) 59, 324
PstI CTGCAG 1 cut(s) 667
PsuI RGATCY 2 cut(s) 76, 195
RsaI GTAC 1 cut(s) 413
RsaNI GTAC 1 cut(s) 412
RseI CAYNNNNRTG 3 cut(s) 188, 477, 492
SatI GCNGC 4 cut(s) 252, 465, 635, 675
Sau3AI GATC 3 cut(s) 76, 195, 638
SchI GAGTC 1 cut(s) 250
ScrFI CCNGG 2 cut(s) 61, 326
SduI GDGCHC 2 cut(s) 539, 550
SfaNI GCATC 2 cut(s) 263, 428
SfcI CTRYAG 2 cut(s) 600, 663
SfuI TTCGAA 2 cut(s) 11, 628
SmiMI CAYNNNNRTG 3 cut(s) 188, 477, 492
SmlI CTYRAG 1 cut(s) 209
SmoI CTYRAG 1 cut(s) 209
Sse9I AATT 2 cut(s) 355, 624
SsiI CCGC 2 cut(s) 374, 634
SspMI CTAG 3 cut(s) 117, 165, 290
StyD4I CCNGG 2 cut(s) 59, 324
StyI CCWWGG 2 cut(s) 183, 472
TaqI TCGA 3 cut(s) 11, 244, 628
TasI AATT 2 cut(s) 355, 624
TatI WGTACW 1 cut(s) 411
TauI GCSGC 1 cut(s) 637
TfiI GAWTC 7 cut(s) 8, 13, 29, 88, 140, 233, 339
TscAI CASTG 1 cut(s) 412
TseFI GTSAC 3 cut(s) 147, 432, 587
TseI GCWGC 3 cut(s) 251, 464, 674
Tsp45I GTSAC 3 cut(s) 147, 432, 587
TspDTI ATGAA 2 cut(s) 302, 348
TspGWI ACGGA 2 cut(s) 20, 596
TspRI CASTG 1 cut(s) 412
XapI RAATTY 1 cut(s) 355
XceI RCATGY 1 cut(s) 374
XspI CTAG 3 cut(s) 117, 165, 290
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.