pycom02g23680

Protein of unknown function, DUF617

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0015467
Physical Location & Seq
Reverse (-)
21835927 .. 21837144
1218 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g23680.1

Sequence Viewer

Length: 870 bp
ATGACAAATGACATGAATGATCGAACACCACTCTCAACCTATTCGATCGATTCTGTTATGGATATGCTCTCAAACATTGCCGTCCCCAGTGATTCAACAATCCACCTACCACCAGTTTTTCTTATGGCAGCAGCGGAAACACAGCCGCCACCGCCACCGCTACCAGCATCGTCACCACGATCTCAAAACCCCAAACCAGCTACACCAACGGTGAATACACCTCCGCCTATCTCCCTCCAACCATCCACGAAAAAAGGTCCATCAAAACCTACAAAACTATTCCGGCGCTTTCGCGCTGTTTTCCGGTCATTTCCAATCATATCTCCTTCATGCAAGATTCCTGTTTCACTCCACGGAAACCGTATTGGAGACGGACACCTCCATGGAGGGACACGGATGACCGGAACCCTATTTGGGTGCCGTAAAGCTAGGGTTAACCTCGTTATCCAGGAAAGCCCTAGATGTCTTCCCATGCTTGTCCTTGAGCTTGGGATACCCACAGGAAAGCTCCTCCAAGATATGGGAATGGGGCTTGTTAGAATAGCCCTAGAGTGCGAAAAGAAACCGGGCCAGAAGATGAAGGTCGTTGATGAGCCGATATGGGTGTTATATTGCAATGGGAAGAAATCAGGGTACGGAGTTAGAAGGGAGCCGACGGAGGATGACTTGAGTGTGATGCAAGTGTTGCATGCAGTGTCCATGGGAGCTGGGGTGCTACCAAATGAAACTGAGTTGCCAGATGGTGACCTAATGTACATGCGAGCACAGTTCGAACGTGTTGTTGGGTCTAGGGATTCGGAGACATATTATATGATGAATCCGGATGGAAACAATGGCCCTGAGCTCAGTGTGTTCTTCGTCAGGGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF617

Protein Analysis

290

Amino Acids

31.71

Weight (kDa)

8.95

Isoelectric Point (pI)

48.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF617 PF04759 133 - 288 3e-68 Protein of unknown function, DUF617
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015467)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G06990
fragaria_vesca FvH4_7g03890
malus_domestica MD07G1043800.v1.1
prunus_persica Prupe.2G043700_v2.0.a1
pyrus_communis pycom02g23680 pycom07g03140
rosa_chinensis RchiOBHm_Chr1g0327341
rosa_laevigata RLG00000029997
rosa_multiflora Rmu_sc0003600.1_g000036
rosa_roxburghii Rroxscaffold_4G00322730
rosa_rugosa Rorug01G0066800
rosa_samantha Rh1AG083500 Rh1BG066400 Rh1CG081400 Rh1DG087800
rosa_wichuraiana Rw1G006560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 417
AccB7I CCANNNNNTGG 1 cut(s) 520
AccII CGCG 1 cut(s) 294
AccIII TCCGGA 1 cut(s) 820
AciI CCGC 5 cut(s) 134, 146, 152, 158, 224
AfaI GTAC 2 cut(s) 635, 755
AfiI CCNNNNNNNGG 2 cut(s) 414, 520
AflIII ACRYGT 1 cut(s) 775
AgsI TTSAA 1 cut(s) 96
AjnI CCWGG 1 cut(s) 447
AjuI GAANNNNNNNTTGG 2 cut(s) 765, 797
AluBI AGCT 6 cut(s) 200, 428, 487, 508, 707, 844
AluI AGCT 6 cut(s) 200, 428, 487, 508, 707, 844
Alw21I GWGCWC 2 cut(s) 766, 846
Alw26I GTCTC 2 cut(s) 363, 794
Aor13HI TCCGGA 1 cut(s) 820
AoxI GGCC 2 cut(s) 568, 835
ApeKI GCWGC 2 cut(s) 128, 131
AspLEI GCGC 2 cut(s) 288, 296
AspS9I GGNCC 3 cut(s) 257, 568, 836
AsuC2I CCSGG 1 cut(s) 567
AsuHPI GGTGA 3 cut(s) 165, 223, 755
AsuII TTCGAA 1 cut(s) 771
AvaII GGWCC 1 cut(s) 257
BanI GGYRCC 1 cut(s) 417
BanII GRGCYC 1 cut(s) 846
BbsI GAAGAC 1 cut(s) 458
Bbv12I GWGCWC 2 cut(s) 766, 846
BbvI GCAGC 2 cut(s) 140, 143
BccI CCATC 4 cut(s) 250, 268, 734, 818
BceAI ACGGC 2 cut(s) 65, 405
BciT130I CCWGG 1 cut(s) 449
BciVI GTATCC 1 cut(s) 486
BcnI CCSGG 1 cut(s) 567
BcoDI GTCTC 2 cut(s) 363, 794
BfaI CTAG 4 cut(s) 429, 459, 548, 789
BfoI RGCGCY 1 cut(s) 289
BfuI GTATCC 1 cut(s) 486
BisI GCNGC 3 cut(s) 129, 132, 146
BlsI GCNGC 3 cut(s) 130, 133, 147
Bme1390I CCNGG 2 cut(s) 449, 567
Bme18I GGWCC 1 cut(s) 257
BmgT120I GGNCC 3 cut(s) 257, 568, 836
BmiI GGNNCC 3 cut(s) 406, 419, 651
BmrFI CCNGG 2 cut(s) 449, 567
BmrI ACTGGG 1 cut(s) 81
BmsI GCATC 2 cut(s) 176, 666
BmuI ACTGGG 1 cut(s) 81
BpiI GAAGAC 1 cut(s) 458
Bpu10I CCTNAGC 1 cut(s) 840
Bpu14I TTCGAA 1 cut(s) 771
BpuEI CTTGAG 2 cut(s) 503, 688
BpuMI CCSGG 1 cut(s) 567
Bsa29I ATCGAT 1 cut(s) 48
BsaJI CCNNGG 3 cut(s) 352, 382, 699
BsaWI WCCGGW 3 cut(s) 303, 401, 820
BsaXI ACNNNNNCTCC 2 cut(s) 696, 726
Bsc4I CCNNNNNNNGG 2 cut(s) 414, 520
Bse1I ACTGG 2 cut(s) 87, 113
Bse3DI GCAATG 2 cut(s) 75, 622
BseAI TCCGGA 1 cut(s) 820
BseBI CCWGG 1 cut(s) 449
BseCI ATCGAT 1 cut(s) 48
BseDI CCNNGG 3 cut(s) 352, 382, 699
BseGI GGATG 4 cut(s) 242, 402, 667, 829
BseLI CCNNNNNNNGG 2 cut(s) 414, 520
BseMI GCAATG 2 cut(s) 75, 622
BseMII CTCAG 3 cut(s) 720, 831, 859
BseNI ACTGG 2 cut(s) 87, 113
BseRI GAGGAG 1 cut(s) 500
BseXI GCAGC 2 cut(s) 140, 143
BseYI CCCAGC 1 cut(s) 707
Bsh1236I CGCG 1 cut(s) 294
Bsh1285I CGRYCG 1 cut(s) 48
BshFI GGCC 2 cut(s) 570, 837
BshNI GGYRCC 1 cut(s) 417
BshVI ATCGAT 1 cut(s) 48
BsiEI CGRYCG 1 cut(s) 48
BsiHKAI GWGCWC 2 cut(s) 766, 846
BsiSI CCGG 5 cut(s) 283, 304, 402, 566, 821
BslFI GGGAC 2 cut(s) 68, 403
BslI CCNNNNNNNGG 2 cut(s) 414, 520
BsmAI GTCTC 2 cut(s) 363, 794
BsmBI CGTCTC 1 cut(s) 363
BsmFI GGGAC 2 cut(s) 68, 403
BsnI GGCC 2 cut(s) 570, 837
Bsp119I TTCGAA 1 cut(s) 771
Bsp1286I GDGCHC 2 cut(s) 766, 846
Bsp13I TCCGGA 1 cut(s) 820
Bsp1407I TGTACA 1 cut(s) 753
Bsp143I GATC 3 cut(s) 19, 45, 179
Bsp19I CCATGG 2 cut(s) 382, 699
BspACI CCGC 5 cut(s) 134, 146, 152, 158, 224
BspANI GGCC 2 cut(s) 570, 837
BspCNI CTCAG 3 cut(s) 721, 832, 858
BspDI ATCGAT 1 cut(s) 48
BspEI TCCGGA 1 cut(s) 820
BspFNI CGCG 1 cut(s) 294
BspLI GGNNCC 3 cut(s) 406, 419, 651
BspT104I TTCGAA 1 cut(s) 771
BspT107I GGYRCC 1 cut(s) 417
BsrDI GCAATG 2 cut(s) 75, 622
BsrGI TGTACA 1 cut(s) 753
BsrI ACTGG 2 cut(s) 87, 113
BssECI CCNNGG 3 cut(s) 352, 382, 699
BssMI GATC 3 cut(s) 19, 45, 179
BssT1I CCWWGG 2 cut(s) 382, 699
Bst2UI CCWGG 1 cut(s) 449
Bst4CI ACNGT 3 cut(s) 211, 362, 768
BstAPI GCANNNNNTGC 1 cut(s) 685
BstAUI TGTACA 1 cut(s) 753
BstBI TTCGAA 1 cut(s) 771
BstC8I GCNNGC 2 cut(s) 690, 762
BstDEI CTNAG 3 cut(s) 729, 840, 845
BstDSI CCRYGG 3 cut(s) 352, 382, 699
BstEII GGTNACC 1 cut(s) 743
BstF5I GGATG 4 cut(s) 242, 402, 667, 829
BstFNI CGCG 1 cut(s) 294
BstH2I RGCGCY 1 cut(s) 289
BstHHI GCGC 2 cut(s) 288, 296
BstKTI GATC 3 cut(s) 22, 48, 182
BstMAI GTCTC 2 cut(s) 363, 794
BstMBI GATC 3 cut(s) 19, 45, 179
BstMCI CGRYCG 1 cut(s) 48
BstMWI GCNNNNNNNGC 2 cut(s) 151, 685
BstNI CCWGG 1 cut(s) 449
BstNSI RCATGY 2 cut(s) 692, 760
BstPI GGTNACC 1 cut(s) 743
BstSCI CCNGG 2 cut(s) 447, 565
BstUI CGCG 1 cut(s) 294
BstV1I GCAGC 2 cut(s) 140, 143
BstV2I GAAGAC 1 cut(s) 458
Bsu15I ATCGAT 1 cut(s) 48
BsuI GTATCC 1 cut(s) 486
BsuRI GGCC 2 cut(s) 570, 837
BsuTUI ATCGAT 1 cut(s) 48
BtgI CCRYGG 3 cut(s) 352, 382, 699
BtsCI GGATG 4 cut(s) 242, 402, 667, 829
BtsI GCAGTG 1 cut(s) 699
BtsIMutI CAGTG 3 cut(s) 94, 699, 853
Cac8I GCNNGC 2 cut(s) 690, 762
CfoI GCGC 2 cut(s) 288, 296
Cfr13I GGNCC 3 cut(s) 257, 568, 836
ClaI ATCGAT 1 cut(s) 48
Csp6I GTAC 2 cut(s) 634, 754
CviAII CATG 7 cut(s) 13, 330, 383, 472, 689, 700, 757
CviQI GTAC 2 cut(s) 634, 754
DdeI CTNAG 3 cut(s) 729, 840, 845
DpnI GATC 3 cut(s) 21, 47, 181
DpnII GATC 3 cut(s) 19, 45, 179
EciI GGCGGA 1 cut(s) 213
Ecl136II GAGCTC 1 cut(s) 844
Eco130I CCWWGG 2 cut(s) 382, 699
Eco24I GRGCYC 1 cut(s) 846
Eco47I GGWCC 1 cut(s) 257
Eco53kI GAGCTC 1 cut(s) 844
Eco91I GGTNACC 1 cut(s) 743
EcoICRI GAGCTC 1 cut(s) 844
EcoO65I GGTNACC 1 cut(s) 743
EcoRII CCWGG 1 cut(s) 447
EcoT14I CCWWGG 2 cut(s) 382, 699
EcoT38I GRGCYC 1 cut(s) 846
ErhI CCWWGG 2 cut(s) 382, 699
Esp3I CGTCTC 1 cut(s) 363
FaeI CATG 7 cut(s) 16, 333, 386, 475, 692, 703, 760
FaqI GGGAC 2 cut(s) 68, 403
FatI CATG 7 cut(s) 12, 329, 382, 471, 688, 699, 756
Fnu4HI GCNGC 3 cut(s) 129, 132, 146
FokI GGATG 4 cut(s) 229, 409, 674, 836
FriOI GRGCYC 1 cut(s) 846
Fsp4HI GCNGC 3 cut(s) 129, 132, 146
FspBI CTAG 4 cut(s) 429, 459, 548, 789
GlaI GCGC 2 cut(s) 287, 295
GluI GCNGC 3 cut(s) 129, 132, 146
GsaI CCCAGC 1 cut(s) 711
HaeII RGCGCY 1 cut(s) 289
HaeIII GGCC 2 cut(s) 570, 837
HapII CCGG 5 cut(s) 283, 304, 402, 566, 821
HhaI GCGC 2 cut(s) 288, 296
Hin1II CATG 7 cut(s) 16, 333, 386, 475, 692, 703, 760
Hin6I GCGC 2 cut(s) 286, 294
HinP1I GCGC 2 cut(s) 286, 294
HincII GTYRAC 1 cut(s) 436
HindII GTYRAC 1 cut(s) 436
HinfI GANTC 5 cut(s) 50, 92, 337, 794, 817
HpaI GTTAAC 1 cut(s) 436
HpaII CCGG 5 cut(s) 283, 304, 402, 566, 821
HphI GGTGA 3 cut(s) 165, 223, 755
Hpy166II GTNNAC 1 cut(s) 436
Hpy188I TCNGA 1 cut(s) 799
Hpy188III TCNNGA 1 cut(s) 821
Hpy8I GTNNAC 1 cut(s) 436
Hpy99I CGWCG 1 cut(s) 658
HpyAV CCTTC 3 cut(s) 336, 574, 639
HpyCH4III ACNGT 3 cut(s) 211, 362, 768
HpyCH4IV ACGT 1 cut(s) 775
HpyCH4V TGCA 5 cut(s) 333, 615, 679, 688, 692
HpyF10VI GCNNNNNNNGC 2 cut(s) 151, 685
HpyF3I CTNAG 3 cut(s) 729, 840, 845
HpySE526I ACGT 1 cut(s) 775
Hsp92II CATG 7 cut(s) 16, 333, 386, 475, 692, 703, 760
HspAI GCGC 2 cut(s) 286, 294
Kpn2I TCCGGA 1 cut(s) 820
KspAI GTTAAC 1 cut(s) 436
Kzo9I GATC 3 cut(s) 19, 45, 179
LmnI GCTCC 3 cut(s) 513, 649, 704
Lsp1109I GCAGC 2 cut(s) 140, 143
LweI GCATC 2 cut(s) 176, 666
MaeI CTAG 4 cut(s) 429, 459, 548, 789
MaeII ACGT 1 cut(s) 775
MaeIII GTNAC 2 cut(s) 171, 743
MalI GATC 3 cut(s) 21, 47, 181
MboI GATC 3 cut(s) 19, 45, 179
MboII GAAGA 4 cut(s) 458, 586, 634, 847
MhlI GDGCHC 2 cut(s) 766, 846
MmeI TCCRAC 1 cut(s) 262
MnlI CCTC 7 cut(s) 231, 245, 380, 389, 449, 521, 652
MroI TCCGGA 1 cut(s) 820
MseI TTAA 1 cut(s) 435
MslI CAYNNNNRTG 1 cut(s) 381
MspA1I CMGCKG 1 cut(s) 134
MspI CCGG 5 cut(s) 283, 304, 402, 566, 821
MspR9I CCNGG 2 cut(s) 449, 567
MvaI CCWGG 1 cut(s) 449
MvnI CGCG 1 cut(s) 294
MwoI GCNNNNNNNGC 2 cut(s) 151, 685
NciI CCSGG 1 cut(s) 567
NcoI CCATGG 2 cut(s) 382, 699
NdeII GATC 3 cut(s) 19, 45, 179
NlaIII CATG 7 cut(s) 16, 333, 386, 475, 692, 703, 760
NlaIV GGNNCC 3 cut(s) 406, 419, 651
NmuCI GTSAC 2 cut(s) 171, 743
NspI RCATGY 2 cut(s) 692, 760
NspV TTCGAA 1 cut(s) 771
PaeI GCATGC 1 cut(s) 692
PfeI GAWTC 5 cut(s) 50, 92, 337, 794, 817
PflMI CCANNNNNTGG 1 cut(s) 520
PfoI TCCNGGA 1 cut(s) 447
PkrI GCNGC 3 cut(s) 130, 133, 147
Ple19I CGATCG 1 cut(s) 48
Psp124BI GAGCTC 1 cut(s) 846
Psp6I CCWGG 1 cut(s) 447
PspEI GGTNACC 1 cut(s) 743
PspFI CCCAGC 1 cut(s) 707
PspGI CCWGG 1 cut(s) 447
PspN4I GGNNCC 3 cut(s) 406, 419, 651
PspPI GGNCC 3 cut(s) 257, 568, 836
PvuI CGATCG 1 cut(s) 48
RsaI GTAC 2 cut(s) 635, 755
RsaNI GTAC 2 cut(s) 634, 754
RseI CAYNNNNRTG 1 cut(s) 381
SacI GAGCTC 1 cut(s) 846
SaqAI TTAA 1 cut(s) 435
SatI GCNGC 3 cut(s) 129, 132, 146
Sau3AI GATC 3 cut(s) 19, 45, 179
Sau96I GGNCC 3 cut(s) 257, 568, 836
ScrFI CCNGG 2 cut(s) 449, 567
SduI GDGCHC 2 cut(s) 766, 846
SfaNI GCATC 2 cut(s) 176, 666
SfuI TTCGAA 1 cut(s) 771
SinI GGWCC 1 cut(s) 257
SmiMI CAYNNNNRTG 1 cut(s) 381
SmlI CTYRAG 2 cut(s) 482, 667
SmoI CTYRAG 2 cut(s) 482, 667
SphI GCATGC 1 cut(s) 692
SsiI CCGC 5 cut(s) 134, 146, 152, 158, 224
SspMI CTAG 4 cut(s) 429, 459, 548, 789
SstI GAGCTC 1 cut(s) 846
StyD4I CCNGG 2 cut(s) 447, 565
StyI CCWWGG 2 cut(s) 382, 699
TaaI ACNGT 3 cut(s) 211, 362, 768
TaiI ACGT 1 cut(s) 778
TaqI TCGA 4 cut(s) 22, 44, 48, 771
TatI WGTACW 1 cut(s) 753
TauI GCSGC 1 cut(s) 148
TfiI GAWTC 5 cut(s) 50, 92, 337, 794, 817
Tru1I TTAA 1 cut(s) 435
Tru9I TTAA 1 cut(s) 435
TscAI CASTG 3 cut(s) 94, 699, 853
TseFI GTSAC 2 cut(s) 171, 743
TseI GCWGC 2 cut(s) 128, 131
Tsp45I GTSAC 2 cut(s) 171, 743
TspDTI ATGAA 5 cut(s) 29, 318, 593, 738, 830
TspGWI ACGGA 5 cut(s) 369, 387, 409, 651, 671
TspRI CASTG 3 cut(s) 94, 699, 853
Van91I CCANNNNNTGG 1 cut(s) 520
VpaK11BI GGWCC 1 cut(s) 257
XceI RCATGY 2 cut(s) 692, 760
XspI CTAG 4 cut(s) 429, 459, 548, 789
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.