Rh1BG066400

Protein of unknown function, DUF617

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0015467
Physical Location & Seq
Forward (+)
10116333 .. 10117605
1273 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG066400.1

Sequence Viewer

Length: 672 bp
ATGAAAAATCACCACCTGCCATCTCCCTCCAACCCGCATCAAACAAAAAAAGGACCCTCAAAATCCAGAAAATTTTTCCGGCGCTTCCGTGCTGTTTTCCGGTCGTTTCCCATCATAGCCCCGTCGTGCAAAATCCCGGTCTCTCTCCACCACGGGAGCCGGATCGGGGAAGGACACATCCACGGTGGTATCAGGATGACTGGAACCCTATTTGGGAACCGTAAATCTAGGGTGAGCATTGCAATCCAAGAAAGCCCCAAGTGCCTCCCCCTTCTTGTAATTGAGCTAGGGATTCCCACAGGAAAGCTTCTCCAGGACATGGGAGTCGGGTTCGTGAGAATAGCCCTCGAGTGTGAGAAGAGACCAGGTGATAAGACAAAGATCGTCGATGAACCCATATGGATGTTGTATTGCAATGGGAAGAAATCGGGGTATGGGGTTCGGAGGGAAGCGACGGACGATGACTTGAGTGTCATGCAAGTATTGCATGCAATTTCTATGGGAGCCGGGGTATTACCAAACGACGGAGACGAGTTGCCGGACGGTGAGTTCATGTACATGAGAGCACAGTTCGAGCGTGTGATCGGGTCCAAGGATTCGGAAACGTACTACATGTTGAATCCCGAAGGGAATAATGGGCCTGAGCTCAGTGTATTCTTCGTAAGGGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF617

Protein Analysis

223

Amino Acids

24.86

Weight (kDa)

9.41

Isoelectric Point (pI)

46.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF617 PF04759 66 - 222 3.5e-67 Protein of unknown function, DUF617
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015467)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G06990
fragaria_vesca FvH4_7g03890
malus_domestica MD07G1043800.v1.1
prunus_persica Prupe.2G043700_v2.0.a1
pyrus_communis pycom02g23680 pycom07g03140
rosa_chinensis RchiOBHm_Chr1g0327341
rosa_laevigata RLG00000029997
rosa_multiflora Rmu_sc0003600.1_g000036
rosa_roxburghii Rroxscaffold_4G00322730
rosa_rugosa Rorug01G0066800
rosa_samantha Rh1AG083500 Rh1BG066400 Rh1CG081400 Rh1DG087800
rosa_wichuraiana Rw1G006560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 24
AasI GACNNNNNNGTC 2 cut(s) 323, 470
Acc36I ACCTGC 1 cut(s) 24
AccB7I CCANNNNNTGG 1 cut(s) 319
AciI CCGC 1 cut(s) 35
AclWI GGATC 1 cut(s) 170
AcsI RAATTY 1 cut(s) 71
AfaI GTAC 2 cut(s) 557, 608
AfiI CCNNNNNNNGG 4 cut(s) 166, 213, 319, 524
AflIII ACRYGT 1 cut(s) 612
AgsI TTSAA 1 cut(s) 619
AjnI CCWGG 2 cut(s) 312, 364
AloI GAACNNNNNNTCC 2 cut(s) 533, 565
AluBI AGCT 3 cut(s) 286, 307, 646
AluI AGCT 3 cut(s) 286, 307, 646
Alw21I GWGCWC 2 cut(s) 568, 648
Alw26I GTCTC 3 cut(s) 145, 355, 522
AlwI GGATC 1 cut(s) 170
Ama87I CYCGRG 1 cut(s) 347
AoxI GGCC 1 cut(s) 638
ApoI RAATTY 1 cut(s) 71
ArsI GACNNNNNNTTYG 2 cut(s) 123, 155
AspLEI GCGC 1 cut(s) 84
AspS9I GGNCC 3 cut(s) 53, 588, 638
AsuC2I CCSGG 2 cut(s) 137, 508
AsuHPI GGTGA 3 cut(s) 244, 380, 557
AvaI CYCGRG 1 cut(s) 347
AvaII GGWCC 2 cut(s) 53, 588
BaeI ACNNNNGTAYC 2 cut(s) 172, 205
BanII GRGCYC 1 cut(s) 648
Bbv12I GWGCWC 2 cut(s) 568, 648
BccI CCATC 2 cut(s) 28, 119
BciT130I CCWGG 2 cut(s) 314, 366
BcnI CCSGG 2 cut(s) 137, 508
BcoDI GTCTC 3 cut(s) 145, 355, 522
BfaI CTAG 3 cut(s) 228, 287, 670
BfoI RGCGCY 1 cut(s) 85
BfuAI ACCTGC 1 cut(s) 24
Bme1390I CCNGG 4 cut(s) 137, 314, 366, 508
Bme18I GGWCC 2 cut(s) 53, 588
BmeT110I CYCGRG 1 cut(s) 347
BmgT120I GGNCC 3 cut(s) 53, 588, 638
BmiI GGNNCC 6 cut(s) 55, 158, 205, 218, 505, 589
BmrFI CCNGG 4 cut(s) 137, 314, 366, 508
BmsI GCATC 1 cut(s) 46
BpmI CTGGAG 1 cut(s) 296
Bpu10I CCTNAGC 1 cut(s) 642
BpuEI CTTGAG 1 cut(s) 487
BpuMI CCSGG 2 cut(s) 137, 508
BsaI GGTCTC 2 cut(s) 145, 355
BsaJI CCNNGG 4 cut(s) 151, 181, 507, 591
BsaWI WCCGGW 1 cut(s) 99
BsaXI ACNNNNNCTCC 2 cut(s) 495, 525
Bsc4I CCNNNNNNNGG 4 cut(s) 166, 213, 319, 524
Bse1I ACTGG 1 cut(s) 205
Bse3DI GCAATG 2 cut(s) 237, 421
BseBI CCWGG 2 cut(s) 314, 366
BseDI CCNNGG 4 cut(s) 151, 181, 507, 591
BseGI GGATG 3 cut(s) 177, 201, 408
BseLI CCNNNNNNNGG 4 cut(s) 166, 213, 319, 524
BseMI GCAATG 2 cut(s) 237, 421
BseMII CTCAG 2 cut(s) 633, 661
BseNI ACTGG 1 cut(s) 205
Bsh1285I CGRYCG 1 cut(s) 104
BshFI GGCC 1 cut(s) 640
BsiEI CGRYCG 1 cut(s) 104
BsiHKAI GWGCWC 2 cut(s) 568, 648
BsiHKCI CYCGRG 1 cut(s) 347
BsiSI CCGG 6 cut(s) 79, 100, 137, 160, 507, 539
BslI CCNNNNNNNGG 4 cut(s) 166, 213, 319, 524
BsmAI GTCTC 3 cut(s) 145, 355, 522
BsmBI CGTCTC 1 cut(s) 522
BsnI GGCC 1 cut(s) 640
Bso31I GGTCTC 2 cut(s) 145, 355
BsoBI CYCGRG 1 cut(s) 347
Bsp1286I GDGCHC 2 cut(s) 568, 648
Bsp1407I TGTACA 1 cut(s) 555
Bsp143I GATC 3 cut(s) 162, 381, 582
BspACI CCGC 1 cut(s) 35
BspANI GGCC 1 cut(s) 640
BspCNI CTCAG 2 cut(s) 634, 660
BspLI GGNNCC 6 cut(s) 55, 158, 205, 218, 505, 589
BspMI ACCTGC 1 cut(s) 24
BspPI GGATC 1 cut(s) 170
BspTNI GGTCTC 2 cut(s) 145, 355
BsrDI GCAATG 2 cut(s) 237, 421
BsrGI TGTACA 1 cut(s) 555
BsrI ACTGG 1 cut(s) 205
BssECI CCNNGG 4 cut(s) 151, 181, 507, 591
BssMI GATC 3 cut(s) 162, 381, 582
BssT1I CCWWGG 1 cut(s) 591
Bst2UI CCWGG 2 cut(s) 314, 366
Bst4CI ACNGT 4 cut(s) 185, 221, 545, 570
Bst6I CTCTTC 1 cut(s) 353
BstAPI GCANNNNNTGC 1 cut(s) 484
BstAUI TGTACA 1 cut(s) 555
BstC8I GCNNGC 1 cut(s) 489
BstDEI CTNAG 2 cut(s) 642, 647
BstDSI CCRYGG 2 cut(s) 151, 181
BstF5I GGATG 3 cut(s) 177, 201, 408
BstH2I RGCGCY 1 cut(s) 85
BstHHI GCGC 1 cut(s) 84
BstKTI GATC 3 cut(s) 165, 384, 585
BstMAI GTCTC 3 cut(s) 145, 355, 522
BstMBI GATC 3 cut(s) 162, 381, 582
BstMCI CGRYCG 1 cut(s) 104
BstMWI GCNNNNNNNGC 2 cut(s) 261, 484
BstNI CCWGG 2 cut(s) 314, 366
BstNSI RCATGY 2 cut(s) 491, 616
BstSCI CCNGG 4 cut(s) 135, 312, 364, 506
BsuRI GGCC 1 cut(s) 640
BtgI CCRYGG 2 cut(s) 151, 181
BtsCI GGATG 3 cut(s) 177, 201, 408
BtsIMutI CAGTG 1 cut(s) 655
BveI ACCTGC 1 cut(s) 24
Cac8I GCNNGC 1 cut(s) 489
CfoI GCGC 1 cut(s) 84
Cfr13I GGNCC 3 cut(s) 53, 588, 638
CsiI ACCWGGT 1 cut(s) 364
Csp6I GTAC 2 cut(s) 556, 607
CviAII CATG 6 cut(s) 319, 475, 488, 553, 559, 613
CviJI RGCY 9 cut(s) 119, 159, 255, 286, 307, 344, 506, 640, 646
CviKI_1 RGCY 9 cut(s) 119, 159, 255, 286, 307, 344, 506, 640, 646
CviQI GTAC 2 cut(s) 556, 607
DdeI CTNAG 2 cut(s) 642, 647
DpnI GATC 3 cut(s) 164, 383, 584
DpnII GATC 3 cut(s) 162, 381, 582
DrdI GACNNNNNNGTC 2 cut(s) 323, 470
DseDI GACNNNNNNGTC 2 cut(s) 323, 470
Eam1104I CTCTTC 1 cut(s) 353
EarI CTCTTC 1 cut(s) 353
Ecl136II GAGCTC 1 cut(s) 646
Eco130I CCWWGG 1 cut(s) 591
Eco24I GRGCYC 1 cut(s) 648
Eco31I GGTCTC 2 cut(s) 145, 355
Eco47I GGWCC 2 cut(s) 53, 588
Eco53kI GAGCTC 1 cut(s) 646
Eco88I CYCGRG 1 cut(s) 347
EcoICRI GAGCTC 1 cut(s) 646
EcoO109I RGGNCCY 1 cut(s) 53
EcoRII CCWGG 2 cut(s) 312, 364
EcoT14I CCWWGG 1 cut(s) 591
EcoT38I GRGCYC 1 cut(s) 648
ErhI CCWWGG 1 cut(s) 591
Esp3I CGTCTC 1 cut(s) 522
FaeI CATG 6 cut(s) 322, 478, 491, 556, 562, 616
FatI CATG 6 cut(s) 318, 474, 487, 552, 558, 612
FauI CCCGC 1 cut(s) 42
FauNDI CATATG 1 cut(s) 398
FokI GGATG 3 cut(s) 164, 208, 415
FriOI GRGCYC 1 cut(s) 648
FspBI CTAG 3 cut(s) 228, 287, 670
GlaI GCGC 1 cut(s) 83
GsuI CTGGAG 1 cut(s) 296
HaeII RGCGCY 1 cut(s) 85
HaeIII GGCC 1 cut(s) 640
HapII CCGG 6 cut(s) 79, 100, 137, 160, 507, 539
HhaI GCGC 1 cut(s) 84
Hin1II CATG 6 cut(s) 322, 478, 491, 556, 562, 616
Hin6I GCGC 1 cut(s) 82
HinP1I GCGC 1 cut(s) 82
HindIII AAGCTT 1 cut(s) 305
HinfI GANTC 4 cut(s) 292, 324, 596, 619
HpaII CCGG 6 cut(s) 79, 100, 137, 160, 507, 539
HphI GGTGA 3 cut(s) 244, 380, 557
Hpy188I TCNGA 2 cut(s) 444, 601
Hpy188III TCNNGA 4 cut(s) 66, 193, 334, 623
Hpy99I CGWCG 4 cut(s) 127, 389, 457, 527
HpyAV CCTTC 3 cut(s) 164, 281, 620
HpyCH4III ACNGT 4 cut(s) 185, 221, 545, 570
HpyCH4IV ACGT 1 cut(s) 605
HpyCH4V TGCA 6 cut(s) 129, 242, 414, 478, 487, 491
HpyF10VI GCNNNNNNNGC 2 cut(s) 261, 484
HpyF3I CTNAG 2 cut(s) 642, 647
HpySE526I ACGT 1 cut(s) 605
Hsp92II CATG 6 cut(s) 322, 478, 491, 556, 562, 616
HspAI GCGC 1 cut(s) 82
Kzo9I GATC 3 cut(s) 162, 381, 582
LmnI GCTCC 2 cut(s) 156, 503
LweI GCATC 1 cut(s) 46
MabI ACCWGGT 1 cut(s) 364
MaeI CTAG 3 cut(s) 228, 287, 670
MaeII ACGT 1 cut(s) 605
MalI GATC 3 cut(s) 164, 383, 584
MboI GATC 3 cut(s) 162, 381, 582
MboII GAAGA 3 cut(s) 370, 433, 649
MhlI GDGCHC 2 cut(s) 568, 648
MluCI AATT 3 cut(s) 71, 279, 492
MlyI GAGTC 1 cut(s) 333
MmeI TCCRAC 1 cut(s) 54
MnlI CCTC 5 cut(s) 37, 67, 275, 356, 438
MslI CAYNNNNRTG 2 cut(s) 401, 557
MspI CCGG 6 cut(s) 79, 100, 137, 160, 507, 539
MspR9I CCNGG 4 cut(s) 137, 314, 366, 508
MvaI CCWGG 2 cut(s) 314, 366
MwoI GCNNNNNNNGC 2 cut(s) 261, 484
NciI CCSGG 2 cut(s) 137, 508
NdeI CATATG 1 cut(s) 398
NdeII GATC 3 cut(s) 162, 381, 582
NlaIII CATG 6 cut(s) 322, 478, 491, 556, 562, 616
NlaIV GGNNCC 6 cut(s) 55, 158, 205, 218, 505, 589
NspI RCATGY 2 cut(s) 491, 616
PaeI GCATGC 1 cut(s) 491
PaeR7I CTCGAG 1 cut(s) 347
PaqCI CACCTGC 1 cut(s) 24
PciI ACATGT 1 cut(s) 612
PcsI WCGNNNNNNNCGW 1 cut(s) 528
PfeI GAWTC 3 cut(s) 292, 596, 619
PflMI CCANNNNNTGG 1 cut(s) 319
PfoI TCCNGGA 1 cut(s) 312
PleI GAGTC 1 cut(s) 332
PpsI GAGTC 1 cut(s) 332
PpuMI RGGWCCY 1 cut(s) 53
PscI ACATGT 1 cut(s) 612
Psp124BI GAGCTC 1 cut(s) 648
Psp5II RGGWCCY 1 cut(s) 53
Psp6I CCWGG 2 cut(s) 312, 364
PspGI CCWGG 2 cut(s) 312, 364
PspN4I GGNNCC 6 cut(s) 55, 158, 205, 218, 505, 589
PspPI GGNCC 3 cut(s) 53, 588, 638
PspPPI RGGWCCY 1 cut(s) 53
PspXI VCTCGAGB 1 cut(s) 347
RsaI GTAC 2 cut(s) 557, 608
RsaNI GTAC 2 cut(s) 556, 607
RseI CAYNNNNRTG 2 cut(s) 401, 557
SacI GAGCTC 1 cut(s) 648
Sau3AI GATC 3 cut(s) 162, 381, 582
Sau96I GGNCC 3 cut(s) 53, 588, 638
SchI GAGTC 1 cut(s) 333
ScrFI CCNGG 4 cut(s) 137, 314, 366, 508
SduI GDGCHC 2 cut(s) 568, 648
SetI ASST 6 cut(s) 18, 288, 309, 370, 608, 648
SexAI ACCWGGT 1 cut(s) 364
SfaNI GCATC 1 cut(s) 46
Sfr274I CTCGAG 1 cut(s) 347
SinI GGWCC 2 cut(s) 53, 588
SlaI CTCGAG 1 cut(s) 347
SmiMI CAYNNNNRTG 2 cut(s) 401, 557
SmlI CTYRAG 2 cut(s) 347, 466
SmoI CTYRAG 2 cut(s) 347, 466
SphI GCATGC 1 cut(s) 491
Sse9I AATT 3 cut(s) 71, 279, 492
SsiI CCGC 1 cut(s) 35
SspMI CTAG 3 cut(s) 228, 287, 670
SstI GAGCTC 1 cut(s) 648
StyD4I CCNGG 4 cut(s) 135, 312, 364, 506
StyI CCWWGG 1 cut(s) 591
TaaI ACNGT 4 cut(s) 185, 221, 545, 570
TaiI ACGT 1 cut(s) 608
TaqI TCGA 3 cut(s) 348, 387, 573
TasI AATT 3 cut(s) 71, 279, 492
TatI WGTACW 1 cut(s) 555
TfiI GAWTC 3 cut(s) 292, 596, 619
TscAI CASTG 1 cut(s) 655
TspDTI ATGAA 3 cut(s) 17, 405, 541
TspGWI ACGGA 3 cut(s) 77, 470, 540
TspRI CASTG 1 cut(s) 655
Van91I CCANNNNNTGG 1 cut(s) 319
VpaK11BI GGWCC 2 cut(s) 53, 588
XapI RAATTY 1 cut(s) 71
XceI RCATGY 2 cut(s) 491, 616
XhoI CTCGAG 1 cut(s) 347
XspI CTAG 3 cut(s) 228, 287, 670
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.