Rroxscaffold_4G00322730

Protein of unknown function, DUF617

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0015467
Physical Location & Seq
Reverse (-)
53198087 .. 53198848
762 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00322730.1

Sequence Viewer

Length: 762 bp
ATGGGGGAACCAAAACCACAACCGCAGCCGCTGCCTCCATCCAAGCAGCGGTCTCAAAACCCGGAAGCGAAGTCATCCCCGGCTGTTGCGAATGCACAATCACCACCGGCCATCTCCCTCCAACCCGCATCAAACAAAAAAGGTCCCTCAAAATCCAGAAAACTTTTCCGGCGCTTCCGTGCTGTTTTCCGGTCTTTTCCCATCATAGTCCCGTCGTGCAAAATCCCGGTCTCTCTCCACCACGGGAGCCGGATCGGGGAAGGACACATCCACGGTGGTATCAGGATGACTGGAACCCTATTTGGGAACCGTAAATCTAGGGTGAGCATTGCAATCCAAGAAAGCCCCAAGTGCCTCCCCCTTCTCGTAATTGAGCTAGGGATTCCCACAGGAAAGCTTCTCCAGGACATGGGAGTCGGGTTCGTGAGAATAGCCCTCGAGTGTGAGAAGAGACCAGGTGATAAGACAAAGATCGTCGATGAACCCATATGGATGTTGTATTGCAATGGGAAGAAATCGGGGTATGGGGTTCGGAGGGAAGCGACGGACGATGACTTGAGTGTCATGCAAGTATTGCATGCAATTTCTATGGGTGCCGGGGTATTACCAAACGACGGCGACGAGTTGCCGGACGGTGAGTTCATGTACATGAGAGCACAGTTCGAGCGTGTGATCGGGTCCAAGGATTCGGAAACGTACTACATGTTGAATCCCGATGGGAATAATGGGCCTGAGCTCAGTGTATTCTTCGTAAGGGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF617

Protein Analysis

253

Amino Acids

27.78

Weight (kDa)

9.47

Isoelectric Point (pI)

58.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF617 PF04759 96 - 252 3.7e-67 Protein of unknown function, DUF617
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015467)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G06990
fragaria_vesca FvH4_7g03890
malus_domestica MD07G1043800.v1.1
prunus_persica Prupe.2G043700_v2.0.a1
pyrus_communis pycom02g23680 pycom07g03140
rosa_chinensis RchiOBHm_Chr1g0327341
rosa_laevigata RLG00000029997
rosa_multiflora Rmu_sc0003600.1_g000036
rosa_roxburghii Rroxscaffold_4G00322730
rosa_rugosa Rorug01G0066800
rosa_samantha Rh1AG083500 Rh1BG066400 Rh1CG081400 Rh1DG087800
rosa_wichuraiana Rw1G006560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 413, 560
AccB1I GGYRCC 1 cut(s) 593
AccB7I CCANNNNNTGG 1 cut(s) 409
AciI CCGC 4 cut(s) 23, 29, 49, 126
AclWI GGATC 1 cut(s) 260
AcoI YGGCCR 1 cut(s) 108
AfaI GTAC 2 cut(s) 647, 698
AfiI CCNNNNNNNGG 5 cut(s) 48, 256, 303, 409, 614
AflIII ACRYGT 1 cut(s) 702
AgsI TTSAA 1 cut(s) 709
AjnI CCWGG 2 cut(s) 402, 454
AloI GAACNNNNNNTCC 2 cut(s) 623, 655
AluBI AGCT 3 cut(s) 376, 397, 736
AluI AGCT 3 cut(s) 376, 397, 736
Alw21I GWGCWC 2 cut(s) 658, 738
Alw26I GTCTC 3 cut(s) 57, 235, 445
AlwI GGATC 1 cut(s) 260
AlwNI CAGNNNCTG 1 cut(s) 31
Ama87I CYCGRG 1 cut(s) 437
AoxI GGCC 2 cut(s) 108, 728
ApeKI GCWGC 3 cut(s) 25, 31, 46
ArsI GACNNNNNNTTYG 2 cut(s) 213, 245
AspLEI GCGC 1 cut(s) 174
AspS9I GGNCC 3 cut(s) 143, 678, 728
AsuC2I CCSGG 4 cut(s) 62, 80, 227, 598
AsuHPI GGTGA 4 cut(s) 93, 334, 470, 647
AvaI CYCGRG 1 cut(s) 437
AvaII GGWCC 2 cut(s) 143, 678
BaeI ACNNNNGTAYC 2 cut(s) 262, 295
BanI GGYRCC 1 cut(s) 593
BanII GRGCYC 1 cut(s) 738
Bbv12I GWGCWC 2 cut(s) 658, 738
BbvI GCAGC 3 cut(s) 18, 37, 58
BccI CCATC 4 cut(s) 46, 119, 209, 710
BceAI ACGGC 1 cut(s) 631
BciT130I CCWGG 2 cut(s) 404, 456
BcnI CCSGG 4 cut(s) 62, 80, 227, 598
BcoDI GTCTC 3 cut(s) 57, 235, 445
BfaI CTAG 3 cut(s) 318, 377, 760
BfoI RGCGCY 1 cut(s) 175
BisI GCNGC 4 cut(s) 26, 29, 32, 47
BlsI GCNGC 4 cut(s) 27, 30, 33, 48
Bme1390I CCNGG 6 cut(s) 62, 80, 227, 404, 456, 598
Bme18I GGWCC 2 cut(s) 143, 678
BmeT110I CYCGRG 1 cut(s) 437
BmgT120I GGNCC 3 cut(s) 143, 678, 728
BmiI GGNNCC 7 cut(s) 9, 145, 248, 295, 308, 595, 679
BmrFI CCNGG 6 cut(s) 62, 80, 227, 404, 456, 598
BmsI GCATC 1 cut(s) 137
BpmI CTGGAG 1 cut(s) 386
Bpu10I CCTNAGC 1 cut(s) 732
BpuEI CTTGAG 1 cut(s) 577
BpuMI CCSGG 4 cut(s) 62, 80, 227, 598
BsaI GGTCTC 3 cut(s) 57, 235, 445
BsaJI CCNNGG 5 cut(s) 78, 241, 271, 597, 681
BsaWI WCCGGW 1 cut(s) 189
Bsc4I CCNNNNNNNGG 5 cut(s) 48, 256, 303, 409, 614
Bse118I RCCGGY 1 cut(s) 106
Bse1I ACTGG 1 cut(s) 295
Bse3DI GCAATG 2 cut(s) 327, 511
BseBI CCWGG 2 cut(s) 404, 456
BseDI CCNNGG 5 cut(s) 78, 241, 271, 597, 681
BseGI GGATG 5 cut(s) 38, 74, 267, 291, 498
BseLI CCNNNNNNNGG 5 cut(s) 48, 256, 303, 409, 614
BseMI GCAATG 2 cut(s) 327, 511
BseMII CTCAG 2 cut(s) 723, 751
BseNI ACTGG 1 cut(s) 295
BseXI GCAGC 3 cut(s) 18, 37, 58
BshFI GGCC 2 cut(s) 110, 730
BshNI GGYRCC 1 cut(s) 593
BsiHKAI GWGCWC 2 cut(s) 658, 738
BsiHKCI CYCGRG 1 cut(s) 437
BsiSI CCGG 9 cut(s) 62, 80, 107, 169, 190, 227, 250, 597, 629
BslFI GGGAC 2 cut(s) 129, 194
BslI CCNNNNNNNGG 5 cut(s) 48, 256, 303, 409, 614
BsmAI GTCTC 3 cut(s) 57, 235, 445
BsmFI GGGAC 2 cut(s) 129, 194
BsmI GAATGC 1 cut(s) 97
BsnI GGCC 2 cut(s) 110, 730
Bso31I GGTCTC 3 cut(s) 57, 235, 445
BsoBI CYCGRG 1 cut(s) 437
Bsp1286I GDGCHC 2 cut(s) 658, 738
Bsp1407I TGTACA 1 cut(s) 645
Bsp143I GATC 3 cut(s) 252, 471, 672
BspACI CCGC 4 cut(s) 23, 29, 49, 126
BspANI GGCC 2 cut(s) 110, 730
BspCNI CTCAG 2 cut(s) 724, 750
BspLI GGNNCC 7 cut(s) 9, 145, 248, 295, 308, 595, 679
BspPI GGATC 1 cut(s) 260
BspT107I GGYRCC 1 cut(s) 593
BspTNI GGTCTC 3 cut(s) 57, 235, 445
BsrDI GCAATG 2 cut(s) 327, 511
BsrFI RCCGGY 1 cut(s) 106
BsrGI TGTACA 1 cut(s) 645
BsrI ACTGG 1 cut(s) 295
BssAI RCCGGY 1 cut(s) 106
BssECI CCNNGG 5 cut(s) 78, 241, 271, 597, 681
BssMI GATC 3 cut(s) 252, 471, 672
BssT1I CCWWGG 1 cut(s) 681
Bst2UI CCWGG 2 cut(s) 404, 456
Bst4CI ACNGT 4 cut(s) 275, 311, 635, 660
Bst6I CTCTTC 1 cut(s) 443
BstAPI GCANNNNNTGC 2 cut(s) 31, 574
BstAUI TGTACA 1 cut(s) 645
BstC8I GCNNGC 1 cut(s) 579
BstDEI CTNAG 2 cut(s) 732, 737
BstDSI CCRYGG 2 cut(s) 241, 271
BstF5I GGATG 5 cut(s) 38, 74, 267, 291, 498
BstH2I RGCGCY 1 cut(s) 175
BstHHI GCGC 1 cut(s) 174
BstKTI GATC 3 cut(s) 255, 474, 675
BstMAI GTCTC 3 cut(s) 57, 235, 445
BstMBI GATC 3 cut(s) 252, 471, 672
BstMWI GCNNNNNNNGC 3 cut(s) 31, 351, 574
BstNI CCWGG 2 cut(s) 404, 456
BstNSI RCATGY 2 cut(s) 581, 706
BstSCI CCNGG 6 cut(s) 60, 78, 225, 402, 454, 596
BstV1I GCAGC 3 cut(s) 18, 37, 58
BsuRI GGCC 2 cut(s) 110, 730
BtgI CCRYGG 2 cut(s) 241, 271
BtsCI GGATG 5 cut(s) 38, 74, 267, 291, 498
BtsIMutI CAGTG 1 cut(s) 745
Cac8I GCNNGC 1 cut(s) 579
CaiI CAGNNNCTG 1 cut(s) 31
CfoI GCGC 1 cut(s) 174
Cfr10I RCCGGY 1 cut(s) 106
Cfr13I GGNCC 3 cut(s) 143, 678, 728
CsiI ACCWGGT 1 cut(s) 454
Csp6I GTAC 2 cut(s) 646, 697
CviAII CATG 6 cut(s) 409, 565, 578, 643, 649, 703
CviQI GTAC 2 cut(s) 646, 697
DdeI CTNAG 2 cut(s) 732, 737
DpnI GATC 3 cut(s) 254, 473, 674
DpnII GATC 3 cut(s) 252, 471, 672
DrdI GACNNNNNNGTC 2 cut(s) 413, 560
DseDI GACNNNNNNGTC 2 cut(s) 413, 560
EaeI YGGCCR 1 cut(s) 108
Eam1104I CTCTTC 1 cut(s) 443
EarI CTCTTC 1 cut(s) 443
Ecl136II GAGCTC 1 cut(s) 736
Eco130I CCWWGG 1 cut(s) 681
Eco24I GRGCYC 1 cut(s) 738
Eco31I GGTCTC 3 cut(s) 57, 235, 445
Eco47I GGWCC 2 cut(s) 143, 678
Eco53kI GAGCTC 1 cut(s) 736
Eco88I CYCGRG 1 cut(s) 437
EcoICRI GAGCTC 1 cut(s) 736
EcoO109I RGGNCCY 1 cut(s) 143
EcoRII CCWGG 2 cut(s) 402, 454
EcoT14I CCWWGG 1 cut(s) 681
EcoT38I GRGCYC 1 cut(s) 738
ErhI CCWWGG 1 cut(s) 681
FaeI CATG 6 cut(s) 412, 568, 581, 646, 652, 706
FaqI GGGAC 2 cut(s) 129, 194
FatI CATG 6 cut(s) 408, 564, 577, 642, 648, 702
FauI CCCGC 1 cut(s) 133
FauNDI CATATG 1 cut(s) 488
Fnu4HI GCNGC 4 cut(s) 26, 29, 32, 47
FokI GGATG 5 cut(s) 25, 61, 254, 298, 505
FriOI GRGCYC 1 cut(s) 738
Fsp4HI GCNGC 4 cut(s) 26, 29, 32, 47
FspBI CTAG 3 cut(s) 318, 377, 760
GlaI GCGC 1 cut(s) 173
GluI GCNGC 4 cut(s) 26, 29, 32, 47
GsuI CTGGAG 1 cut(s) 386
HaeII RGCGCY 1 cut(s) 175
HaeIII GGCC 2 cut(s) 110, 730
HapII CCGG 9 cut(s) 62, 80, 107, 169, 190, 227, 250, 597, 629
HhaI GCGC 1 cut(s) 174
Hin1II CATG 6 cut(s) 412, 568, 581, 646, 652, 706
Hin6I GCGC 1 cut(s) 172
HinP1I GCGC 1 cut(s) 172
HindIII AAGCTT 1 cut(s) 395
HinfI GANTC 4 cut(s) 382, 414, 686, 709
HpaII CCGG 9 cut(s) 62, 80, 107, 169, 190, 227, 250, 597, 629
HphI GGTGA 4 cut(s) 93, 334, 470, 647
Hpy188I TCNGA 2 cut(s) 534, 691
Hpy188III TCNNGA 4 cut(s) 156, 283, 424, 713
Hpy99I CGWCG 5 cut(s) 217, 479, 547, 617, 623
HpyAV CCTTC 2 cut(s) 254, 371
HpyCH4III ACNGT 4 cut(s) 275, 311, 635, 660
HpyCH4IV ACGT 1 cut(s) 695
HpyCH4V TGCA 7 cut(s) 95, 219, 332, 504, 568, 577, 581
HpyF10VI GCNNNNNNNGC 3 cut(s) 31, 351, 574
HpyF3I CTNAG 2 cut(s) 732, 737
HpySE526I ACGT 1 cut(s) 695
Hsp92II CATG 6 cut(s) 412, 568, 581, 646, 652, 706
HspAI GCGC 1 cut(s) 172
Kzo9I GATC 3 cut(s) 252, 471, 672
LmnI GCTCC 1 cut(s) 246
Lsp1109I GCAGC 3 cut(s) 18, 37, 58
LweI GCATC 1 cut(s) 137
MabI ACCWGGT 1 cut(s) 454
MaeI CTAG 3 cut(s) 318, 377, 760
MaeII ACGT 1 cut(s) 695
MalI GATC 3 cut(s) 254, 473, 674
MboI GATC 3 cut(s) 252, 471, 672
MboII GAAGA 3 cut(s) 460, 523, 739
MhlI GDGCHC 2 cut(s) 658, 738
MluCI AATT 2 cut(s) 369, 582
MlyI GAGTC 1 cut(s) 423
MmeI TCCRAC 1 cut(s) 145
MnlI CCTC 6 cut(s) 45, 128, 157, 365, 446, 528
MslI CAYNNNNRTG 2 cut(s) 491, 647
MspA1I CMGCKG 2 cut(s) 31, 49
MspI CCGG 9 cut(s) 62, 80, 107, 169, 190, 227, 250, 597, 629
MspR9I CCNGG 6 cut(s) 62, 80, 227, 404, 456, 598
Mva1269I GAATGC 1 cut(s) 97
MvaI CCWGG 2 cut(s) 404, 456
MwoI GCNNNNNNNGC 3 cut(s) 31, 351, 574
NciI CCSGG 4 cut(s) 62, 80, 227, 598
NdeI CATATG 1 cut(s) 488
NdeII GATC 3 cut(s) 252, 471, 672
NlaIII CATG 6 cut(s) 412, 568, 581, 646, 652, 706
NlaIV GGNNCC 7 cut(s) 9, 145, 248, 295, 308, 595, 679
NspI RCATGY 2 cut(s) 581, 706
PaeI GCATGC 1 cut(s) 581
PaeR7I CTCGAG 1 cut(s) 437
PciI ACATGT 1 cut(s) 702
PcsI WCGNNNNNNNCGW 1 cut(s) 618
PctI GAATGC 1 cut(s) 97
PfeI GAWTC 3 cut(s) 382, 686, 709
PflMI CCANNNNNTGG 1 cut(s) 409
PfoI TCCNGGA 1 cut(s) 402
PkrI GCNGC 4 cut(s) 27, 30, 33, 48
PleI GAGTC 1 cut(s) 422
PpsI GAGTC 1 cut(s) 422
PpuMI RGGWCCY 1 cut(s) 143
PscI ACATGT 1 cut(s) 702
Psp124BI GAGCTC 1 cut(s) 738
Psp5II RGGWCCY 1 cut(s) 143
Psp6I CCWGG 2 cut(s) 402, 454
PspGI CCWGG 2 cut(s) 402, 454
PspN4I GGNNCC 7 cut(s) 9, 145, 248, 295, 308, 595, 679
PspPI GGNCC 3 cut(s) 143, 678, 728
PspPPI RGGWCCY 1 cut(s) 143
PspXI VCTCGAGB 1 cut(s) 437
PstNI CAGNNNCTG 1 cut(s) 31
RsaI GTAC 2 cut(s) 647, 698
RsaNI GTAC 2 cut(s) 646, 697
RseI CAYNNNNRTG 2 cut(s) 491, 647
SacI GAGCTC 1 cut(s) 738
SatI GCNGC 4 cut(s) 26, 29, 32, 47
Sau3AI GATC 3 cut(s) 252, 471, 672
Sau96I GGNCC 3 cut(s) 143, 678, 728
SchI GAGTC 1 cut(s) 423
ScrFI CCNGG 6 cut(s) 62, 80, 227, 404, 456, 598
SduI GDGCHC 2 cut(s) 658, 738
SetI ASST 6 cut(s) 145, 378, 399, 460, 698, 738
SexAI ACCWGGT 1 cut(s) 454
SfaNI GCATC 1 cut(s) 137
Sfr274I CTCGAG 1 cut(s) 437
SinI GGWCC 2 cut(s) 143, 678
SlaI CTCGAG 1 cut(s) 437
SmiMI CAYNNNNRTG 2 cut(s) 491, 647
SmlI CTYRAG 2 cut(s) 437, 556
SmoI CTYRAG 2 cut(s) 437, 556
SphI GCATGC 1 cut(s) 581
Sse9I AATT 2 cut(s) 369, 582
SsiI CCGC 4 cut(s) 23, 29, 49, 126
SspMI CTAG 3 cut(s) 318, 377, 760
SstI GAGCTC 1 cut(s) 738
StyD4I CCNGG 6 cut(s) 60, 78, 225, 402, 454, 596
StyI CCWWGG 1 cut(s) 681
TaaI ACNGT 4 cut(s) 275, 311, 635, 660
TaiI ACGT 1 cut(s) 698
TaqI TCGA 3 cut(s) 438, 477, 663
TasI AATT 2 cut(s) 369, 582
TatI WGTACW 1 cut(s) 645
TauI GCSGC 1 cut(s) 31
TfiI GAWTC 3 cut(s) 382, 686, 709
TscAI CASTG 1 cut(s) 745
TseI GCWGC 3 cut(s) 25, 31, 46
TspDTI ATGAA 2 cut(s) 495, 631
TspGWI ACGGA 2 cut(s) 167, 560
TspRI CASTG 1 cut(s) 745
Van91I CCANNNNNTGG 1 cut(s) 409
VpaK11BI GGWCC 2 cut(s) 143, 678
XceI RCATGY 2 cut(s) 581, 706
XhoI CTCGAG 1 cut(s) 437
XspI CTAG 3 cut(s) 318, 377, 760
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.