RLG00000029997

Protein of unknown function, DUF617

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0015467
Physical Location & Seq
Reverse (-)
53079592 .. 53081448
1857 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000029997

Sequence Viewer

Length: 1038 bp
ATGGGGGAACCAAAACCACAACCGCGGCCGCTGCCTCCATCCTCGCCGCGGTCTCAAAACCCGGAAGCGAAGTCATCCCCAGCTGTTGCGAATGCACAATCACCACCTGCCATCTCCCTCCAACCCGCATCAAACAAAAAAGGACCCTCAAAATCCAGAAAACTTTTCCGGCGCTTCCGTGCTGTTTTCCGGTCGTTTCCCATCATAGCCCCGTCGTGCAAAATCCCGGTATCTCTCCACCATGGGAGCCGGATCGGGGAAGGACACATCCACGGTGGTATCAGGATGACTGGAACCCTATTTGGGAACCGCAAATCTAGGGTGAGCATTGCAATCCAAGAAAGCCCCAAGTGCCTCCCCCTTCTCGTAATTGAGCTAGGGATTCCCACAGGAAAGCTTCTCCAGGACATGGGAGTCGGGTTCGTGAGAATAGCCCTCGAGTGTGAGAAGAGACCAGGTGATAAGACAAAGATCGTCGATGAGCCCATATGGATGTTGTATTGCAATGGGAAGAAATCGGGGTATGGGGTTCGGAGGGAAGCGACGGACGATGACTTGAGTGTCATGCAAGTATTGCATGCAATTTCTATGGGAGCCGGGGTATTACCAAACGACGGCGACGAGTTGCCGGACGGTGAGTTCATGTATATGAGAGCACAGTTCGAGCGTGTGATCGGGTCCAAGGATTCGGAAACGTACTACATGTTGAATCCCGATGGGAATAATGGGCCTGAGCTCAGTGTGTTCTTCGAGGGATTGGTGATGTATCGGCCTCGGATTCACAACGGCTCTACTACTTTGTACTTTGGTACTCTTCTCGCACCAGGATCCGGTGCAGTGTTCAACGGTGTTTTGGAGTCTGCTCGTGGTTTCGCTGATGGTTTTGCCAGCAGCTCTAGATTTGGTGTTTCTATGACGATCTCCATTGATTCTCTTACGATTGATCCCGTTGGATTAGCAGCACTCGACACTGACAATTCCGTGCTTCTGGCTTTTCTCCTTGGCACTATTCTTTGGTGGAGGATTGGATCAATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF617

Protein Analysis

346

Amino Acids

37.36

Weight (kDa)

9.12

Isoelectric Point (pI)

49.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF617 PF04759 96 - 250 1.5e-65 Protein of unknown function, DUF617
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015467)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G06990
fragaria_vesca FvH4_7g03890
malus_domestica MD07G1043800.v1.1
prunus_persica Prupe.2G043700_v2.0.a1
pyrus_communis pycom02g23680 pycom07g03140
rosa_chinensis RchiOBHm_Chr1g0327341
rosa_laevigata RLG00000029997
rosa_multiflora Rmu_sc0003600.1_g000036
rosa_roxburghii Rroxscaffold_4G00322730
rosa_rugosa Rorug01G0066800
rosa_samantha Rh1AG083500 Rh1BG066400 Rh1CG081400 Rh1DG087800
rosa_wichuraiana Rw1G006560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 115
AasI GACNNNNNNGTC 2 cut(s) 413, 560
Acc36I ACCTGC 1 cut(s) 115
AccB7I CCANNNNNTGG 1 cut(s) 409
AccII CGCG 2 cut(s) 25, 49
AciI CCGC 7 cut(s) 23, 25, 29, 47, 49, 126, 310
AclWI GGATC 5 cut(s) 260, 822, 835, 938, 1036
AcoI YGGCCR 1 cut(s) 26
AfaI GTAC 3 cut(s) 698, 803, 811
AfiI CCNNNNNNNGG 6 cut(s) 48, 256, 303, 409, 614, 830
AflIII ACRYGT 1 cut(s) 702
AgsI TTSAA 2 cut(s) 709, 844
AjnI CCWGG 3 cut(s) 402, 454, 823
AloI GAACNNNNNNTCC 2 cut(s) 623, 655
AluBI AGCT 5 cut(s) 83, 376, 397, 736, 894
AluI AGCT 5 cut(s) 83, 376, 397, 736, 894
Alw21I GWGCWC 2 cut(s) 658, 738
Alw26I GTCTC 2 cut(s) 57, 445
AlwI GGATC 5 cut(s) 260, 822, 835, 938, 1036
Ama87I CYCGRG 1 cut(s) 437
AoxI GGCC 3 cut(s) 26, 728, 770
ApeKI GCWGC 3 cut(s) 31, 891, 959
AspLEI GCGC 1 cut(s) 174
AspS9I GGNCC 3 cut(s) 143, 678, 728
AsuC2I CCSGG 3 cut(s) 62, 227, 598
AsuHPI GGTGA 5 cut(s) 93, 334, 470, 647, 772
AvaI CYCGRG 1 cut(s) 437
AvaII GGWCC 2 cut(s) 143, 678
BaeI ACNNNNGTAYC 2 cut(s) 262, 295
BamHI GGATCC 1 cut(s) 827
BanII GRGCYC 2 cut(s) 486, 738
BauI CACGAG 1 cut(s) 864
Bbv12I GWGCWC 2 cut(s) 658, 738
BbvI GCAGC 3 cut(s) 18, 903, 971
BccI CCATC 5 cut(s) 46, 119, 209, 710, 872
BceAI ACGGC 2 cut(s) 631, 802
BciT130I CCWGG 3 cut(s) 404, 456, 825
BcnI CCSGG 3 cut(s) 62, 227, 598
BcoDI GTCTC 2 cut(s) 57, 445
BfaI CTAG 3 cut(s) 318, 377, 897
BfoI RGCGCY 1 cut(s) 175
BfuAI ACCTGC 1 cut(s) 115
BisI GCNGC 6 cut(s) 26, 29, 32, 47, 892, 960
BlsI GCNGC 6 cut(s) 27, 30, 33, 48, 893, 961
Bme1390I CCNGG 6 cut(s) 62, 227, 404, 456, 598, 825
Bme18I GGWCC 2 cut(s) 143, 678
BmeT110I CYCGRG 1 cut(s) 437
BmgT120I GGNCC 3 cut(s) 143, 678, 728
BmiI GGNNCC 8 cut(s) 9, 145, 248, 295, 308, 595, 679, 829
BmrFI CCNGG 6 cut(s) 62, 227, 404, 456, 598, 825
BmsI GCATC 1 cut(s) 137
BpmI CTGGAG 1 cut(s) 386
Bpu10I CCTNAGC 1 cut(s) 732
BpuEI CTTGAG 1 cut(s) 577
BpuMI CCSGG 3 cut(s) 62, 227, 598
BsaI GGTCTC 2 cut(s) 57, 445
BsaJI CCNNGG 8 cut(s) 23, 47, 241, 271, 597, 681, 773, 1000
BsaWI WCCGGW 2 cut(s) 189, 830
BsaXI ACNNNNNCTCC 2 cut(s) 585, 615
Bsc4I CCNNNNNNNGG 6 cut(s) 48, 256, 303, 409, 614, 830
Bse1I ACTGG 1 cut(s) 295
Bse3DI GCAATG 2 cut(s) 327, 511
BseBI CCWGG 3 cut(s) 404, 456, 825
BseDI CCNNGG 8 cut(s) 23, 47, 241, 271, 597, 681, 773, 1000
BseGI GGATG 5 cut(s) 38, 74, 267, 291, 498
BseLI CCNNNNNNNGG 6 cut(s) 48, 256, 303, 409, 614, 830
BseMI GCAATG 2 cut(s) 327, 511
BseMII CTCAG 2 cut(s) 723, 751
BseNI ACTGG 1 cut(s) 295
BseX3I CGGCCG 1 cut(s) 26
BseXI GCAGC 3 cut(s) 18, 903, 971
BseYI CCCAGC 1 cut(s) 79
BsgI GTGCAG 1 cut(s) 855
Bsh1236I CGCG 2 cut(s) 25, 49
Bsh1285I CGRYCG 2 cut(s) 29, 194
BshFI GGCC 3 cut(s) 28, 730, 772
BsiEI CGRYCG 2 cut(s) 29, 194
BsiHKAI GWGCWC 2 cut(s) 658, 738
BsiHKCI CYCGRG 1 cut(s) 437
BsiSI CCGG 8 cut(s) 62, 169, 190, 227, 250, 597, 629, 831
BslI CCNNNNNNNGG 6 cut(s) 48, 256, 303, 409, 614, 830
BsmAI GTCTC 2 cut(s) 57, 445
BsmI GAATGC 1 cut(s) 97
BsnI GGCC 3 cut(s) 28, 730, 772
Bso31I GGTCTC 2 cut(s) 57, 445
BsoBI CYCGRG 1 cut(s) 437
Bsp1286I GDGCHC 3 cut(s) 486, 658, 738
Bsp143I GATC 7 cut(s) 252, 471, 672, 827, 918, 943, 1028
Bsp19I CCATGG 1 cut(s) 241
BspACI CCGC 7 cut(s) 23, 25, 29, 47, 49, 126, 310
BspANI GGCC 3 cut(s) 28, 730, 772
BspCNI CTCAG 2 cut(s) 724, 750
BspFNI CGCG 2 cut(s) 25, 49
BspLI GGNNCC 8 cut(s) 9, 145, 248, 295, 308, 595, 679, 829
BspMI ACCTGC 1 cut(s) 115
BspPI GGATC 5 cut(s) 260, 822, 835, 938, 1036
BspTNI GGTCTC 2 cut(s) 57, 445
BsrDI GCAATG 2 cut(s) 327, 511
BsrI ACTGG 1 cut(s) 295
BssECI CCNNGG 8 cut(s) 23, 47, 241, 271, 597, 681, 773, 1000
BssMI GATC 7 cut(s) 252, 471, 672, 827, 918, 943, 1028
BssSI CACGAG 1 cut(s) 864
BssT1I CCWWGG 3 cut(s) 241, 681, 1000
Bst2BI CACGAG 1 cut(s) 864
Bst2UI CCWGG 3 cut(s) 404, 456, 825
Bst4CI ACNGT 4 cut(s) 275, 635, 660, 848
Bst6I CTCTTC 2 cut(s) 443, 819
BstAPI GCANNNNNTGC 1 cut(s) 574
BstC8I GCNNGC 2 cut(s) 579, 889
BstDEI CTNAG 2 cut(s) 732, 737
BstDSI CCRYGG 4 cut(s) 23, 47, 241, 271
BstF5I GGATG 5 cut(s) 38, 74, 267, 291, 498
BstFNI CGCG 2 cut(s) 25, 49
BstH2I RGCGCY 1 cut(s) 175
BstHHI GCGC 1 cut(s) 174
BstKTI GATC 7 cut(s) 255, 474, 675, 830, 921, 946, 1031
BstMAI GTCTC 2 cut(s) 57, 445
BstMBI GATC 7 cut(s) 252, 471, 672, 827, 918, 943, 1028
BstMCI CGRYCG 2 cut(s) 29, 194
BstMWI GCNNNNNNNGC 3 cut(s) 31, 351, 574
BstNI CCWGG 3 cut(s) 404, 456, 825
BstNSI RCATGY 2 cut(s) 581, 706
BstSCI CCNGG 6 cut(s) 60, 225, 402, 454, 596, 823
BstUI CGCG 2 cut(s) 25, 49
BstV1I GCAGC 3 cut(s) 18, 903, 971
BstX2I RGATCY 1 cut(s) 827
BstYI RGATCY 1 cut(s) 827
BstZI CGGCCG 1 cut(s) 26
BsuRI GGCC 3 cut(s) 28, 730, 772
BtgI CCRYGG 4 cut(s) 23, 47, 241, 271
BtsCI GGATG 5 cut(s) 38, 74, 267, 291, 498
BtsI GCAGTG 1 cut(s) 843
BtsIMutI CAGTG 3 cut(s) 745, 843, 969
BveI ACCTGC 1 cut(s) 115
Cac8I GCNNGC 2 cut(s) 579, 889
CciNI GCGGCCGC 1 cut(s) 26
CfoI GCGC 1 cut(s) 174
Cfr13I GGNCC 3 cut(s) 143, 678, 728
Cfr42I CCGCGG 2 cut(s) 26, 50
CsiI ACCWGGT 1 cut(s) 454
Csp6I GTAC 3 cut(s) 697, 802, 810
CviAII CATG 6 cut(s) 242, 409, 565, 578, 643, 703
CviQI GTAC 3 cut(s) 697, 802, 810
DdeI CTNAG 2 cut(s) 732, 737
DpnI GATC 7 cut(s) 254, 473, 674, 829, 920, 945, 1030
DpnII GATC 7 cut(s) 252, 471, 672, 827, 918, 943, 1028
DrdI GACNNNNNNGTC 2 cut(s) 413, 560
DseDI GACNNNNNNGTC 2 cut(s) 413, 560
EaeI YGGCCR 1 cut(s) 26
EagI CGGCCG 1 cut(s) 26
Eam1104I CTCTTC 2 cut(s) 443, 819
EarI CTCTTC 2 cut(s) 443, 819
Ecl136II GAGCTC 1 cut(s) 736
EclXI CGGCCG 1 cut(s) 26
Eco130I CCWWGG 3 cut(s) 241, 681, 1000
Eco24I GRGCYC 2 cut(s) 486, 738
Eco31I GGTCTC 2 cut(s) 57, 445
Eco47I GGWCC 2 cut(s) 143, 678
Eco52I CGGCCG 1 cut(s) 26
Eco53kI GAGCTC 1 cut(s) 736
Eco88I CYCGRG 1 cut(s) 437
EcoICRI GAGCTC 1 cut(s) 736
EcoO109I RGGNCCY 1 cut(s) 143
EcoRII CCWGG 3 cut(s) 402, 454, 823
EcoT14I CCWWGG 3 cut(s) 241, 681, 1000
EcoT38I GRGCYC 2 cut(s) 486, 738
ErhI CCWWGG 3 cut(s) 241, 681, 1000
FaeI CATG 6 cut(s) 245, 412, 568, 581, 646, 706
FatI CATG 6 cut(s) 241, 408, 564, 577, 642, 702
FauI CCCGC 1 cut(s) 133
FauNDI CATATG 1 cut(s) 488
Fnu4HI GCNGC 6 cut(s) 26, 29, 32, 47, 892, 960
FokI GGATG 5 cut(s) 25, 61, 254, 298, 505
FriOI GRGCYC 2 cut(s) 486, 738
Fsp4HI GCNGC 6 cut(s) 26, 29, 32, 47, 892, 960
FspBI CTAG 3 cut(s) 318, 377, 897
GlaI GCGC 1 cut(s) 173
GluI GCNGC 6 cut(s) 26, 29, 32, 47, 892, 960
GsaI CCCAGC 1 cut(s) 83
GsuI CTGGAG 1 cut(s) 386
HaeII RGCGCY 1 cut(s) 175
HaeIII GGCC 3 cut(s) 28, 730, 772
HapII CCGG 8 cut(s) 62, 169, 190, 227, 250, 597, 629, 831
HhaI GCGC 1 cut(s) 174
Hin1II CATG 6 cut(s) 245, 412, 568, 581, 646, 706
Hin6I GCGC 1 cut(s) 172
HinP1I GCGC 1 cut(s) 172
HindIII AAGCTT 1 cut(s) 395
HinfI GANTC 7 cut(s) 382, 414, 686, 709, 778, 857, 929
HpaII CCGG 8 cut(s) 62, 169, 190, 227, 250, 597, 629, 831
HphI GGTGA 5 cut(s) 93, 334, 470, 647, 772
Hpy188I TCNGA 3 cut(s) 534, 691, 777
Hpy188III TCNNGA 5 cut(s) 156, 283, 424, 713, 897
Hpy99I CGWCG 5 cut(s) 217, 479, 547, 617, 623
HpyAV CCTTC 2 cut(s) 254, 371
HpyCH4III ACNGT 4 cut(s) 275, 635, 660, 848
HpyCH4IV ACGT 1 cut(s) 695
HpyCH4V TGCA 8 cut(s) 95, 219, 332, 504, 568, 577, 581, 836
HpyF10VI GCNNNNNNNGC 3 cut(s) 31, 351, 574
HpyF3I CTNAG 2 cut(s) 732, 737
HpySE526I ACGT 1 cut(s) 695
Hsp92II CATG 6 cut(s) 245, 412, 568, 581, 646, 706
HspAI GCGC 1 cut(s) 172
KspI CCGCGG 2 cut(s) 26, 50
Kzo9I GATC 7 cut(s) 252, 471, 672, 827, 918, 943, 1028
LmnI GCTCC 2 cut(s) 246, 593
Lsp1109I GCAGC 3 cut(s) 18, 903, 971
LweI GCATC 1 cut(s) 137
MabI ACCWGGT 1 cut(s) 454
MaeI CTAG 3 cut(s) 318, 377, 897
MaeII ACGT 1 cut(s) 695
MalI GATC 7 cut(s) 254, 473, 674, 829, 920, 945, 1030
MboI GATC 7 cut(s) 252, 471, 672, 827, 918, 943, 1028
MboII GAAGA 4 cut(s) 460, 523, 739, 806
MflI RGATCY 1 cut(s) 827
MhlI GDGCHC 3 cut(s) 486, 658, 738
MluCI AATT 3 cut(s) 369, 582, 976
MlyI GAGTC 2 cut(s) 423, 866
MmeI TCCRAC 2 cut(s) 145, 931
MslI CAYNNNNRTG 2 cut(s) 491, 647
MspA1I CMGCKG 4 cut(s) 25, 31, 49, 83
MspI CCGG 8 cut(s) 62, 169, 190, 227, 250, 597, 629, 831
MspR9I CCNGG 6 cut(s) 62, 227, 404, 456, 598, 825
Mva1269I GAATGC 1 cut(s) 97
MvaI CCWGG 3 cut(s) 404, 456, 825
MvnI CGCG 2 cut(s) 25, 49
MwoI GCNNNNNNNGC 3 cut(s) 31, 351, 574
NciI CCSGG 3 cut(s) 62, 227, 598
NcoI CCATGG 1 cut(s) 241
NdeI CATATG 1 cut(s) 488
NdeII GATC 7 cut(s) 252, 471, 672, 827, 918, 943, 1028
NlaIII CATG 6 cut(s) 245, 412, 568, 581, 646, 706
NlaIV GGNNCC 8 cut(s) 9, 145, 248, 295, 308, 595, 679, 829
NotI GCGGCCGC 1 cut(s) 26
NspI RCATGY 2 cut(s) 581, 706
PaeI GCATGC 1 cut(s) 581
PaeR7I CTCGAG 1 cut(s) 437
PaqCI CACCTGC 1 cut(s) 115
PciI ACATGT 1 cut(s) 702
PcsI WCGNNNNNNNCGW 1 cut(s) 618
PctI GAATGC 1 cut(s) 97
PfeI GAWTC 5 cut(s) 382, 686, 709, 778, 929
PflMI CCANNNNNTGG 1 cut(s) 409
PfoI TCCNGGA 1 cut(s) 402
PkrI GCNGC 6 cut(s) 27, 30, 33, 48, 893, 961
PleI GAGTC 2 cut(s) 422, 865
PpsI GAGTC 2 cut(s) 422, 865
PpuMI RGGWCCY 1 cut(s) 143
PscI ACATGT 1 cut(s) 702
Psp124BI GAGCTC 1 cut(s) 738
Psp5II RGGWCCY 1 cut(s) 143
Psp6I CCWGG 3 cut(s) 402, 454, 823
PspFI CCCAGC 1 cut(s) 79
PspGI CCWGG 3 cut(s) 402, 454, 823
PspN4I GGNNCC 8 cut(s) 9, 145, 248, 295, 308, 595, 679, 829
PspPI GGNCC 3 cut(s) 143, 678, 728
PspPPI RGGWCCY 1 cut(s) 143
PspXI VCTCGAGB 1 cut(s) 437
PsuI RGATCY 1 cut(s) 827
PvuII CAGCTG 1 cut(s) 83
RsaI GTAC 3 cut(s) 698, 803, 811
RsaNI GTAC 3 cut(s) 697, 802, 810
RseI CAYNNNNRTG 2 cut(s) 491, 647
SacI GAGCTC 1 cut(s) 738
SacII CCGCGG 2 cut(s) 26, 50
SatI GCNGC 6 cut(s) 26, 29, 32, 47, 892, 960
Sau3AI GATC 7 cut(s) 252, 471, 672, 827, 918, 943, 1028
Sau96I GGNCC 3 cut(s) 143, 678, 728
SchI GAGTC 2 cut(s) 423, 866
ScrFI CCNGG 6 cut(s) 62, 227, 404, 456, 598, 825
SduI GDGCHC 3 cut(s) 486, 658, 738
SetI ASST 8 cut(s) 85, 109, 378, 399, 460, 698, 738, 896
SexAI ACCWGGT 1 cut(s) 454
SfaNI GCATC 1 cut(s) 137
Sfr274I CTCGAG 1 cut(s) 437
Sfr303I CCGCGG 2 cut(s) 26, 50
SgrBI CCGCGG 2 cut(s) 26, 50
SinI GGWCC 2 cut(s) 143, 678
SlaI CTCGAG 1 cut(s) 437
SmiMI CAYNNNNRTG 2 cut(s) 491, 647
SmlI CTYRAG 2 cut(s) 437, 556
SmoI CTYRAG 2 cut(s) 437, 556
SphI GCATGC 1 cut(s) 581
Sse9I AATT 3 cut(s) 369, 582, 976
SsiI CCGC 7 cut(s) 23, 25, 29, 47, 49, 126, 310
SspMI CTAG 3 cut(s) 318, 377, 897
SstI GAGCTC 1 cut(s) 738
StyD4I CCNGG 6 cut(s) 60, 225, 402, 454, 596, 823
StyI CCWWGG 3 cut(s) 241, 681, 1000
TaaI ACNGT 4 cut(s) 275, 635, 660, 848
TaiI ACGT 1 cut(s) 698
TaqI TCGA 5 cut(s) 438, 477, 663, 750, 966
TasI AATT 3 cut(s) 369, 582, 976
TatI WGTACW 1 cut(s) 801
TauI GCSGC 3 cut(s) 28, 31, 49
TfiI GAWTC 5 cut(s) 382, 686, 709, 778, 929
TscAI CASTG 3 cut(s) 745, 843, 976
TseI GCWGC 3 cut(s) 31, 891, 959
TspDTI ATGAA 1 cut(s) 631
TspGWI ACGGA 3 cut(s) 167, 560, 970
TspRI CASTG 3 cut(s) 745, 843, 976
Van91I CCANNNNNTGG 1 cut(s) 409
VpaK11BI GGWCC 2 cut(s) 143, 678
XbaI TCTAGA 1 cut(s) 896
XceI RCATGY 2 cut(s) 581, 706
XhoI CTCGAG 1 cut(s) 437
XspI CTAG 3 cut(s) 318, 377, 897
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.