pycom07g03140

Protein of unknown function, DUF617

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0015467
Physical Location & Seq
Reverse (-)
2592352 .. 2593556
1205 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g03140.1

Sequence Viewer

Length: 810 bp
ATGAAGTGTCATCTCTCAAGCATTGCCGTCCCGAGCAATTCAACAACCCACTTACCACCAGTTTTTCTTATGGCAGCGGCGGAAACACAACCACCACCGCTACCAGCGTCGTCACCATGGTCCCAAAACCCCAAACCAACTACACCAACGGTGAATAGACCTCCACATATCTCCCTCCAACCATCGACCAAAAAAGGCCCATCAAGATCTAAAAAACTCTTCTGGCGCTTTCGTGCTGTTTTCCGGTCATTTCCAATCATATCTCCTTCATGCAAGATCCCCGTTTCGCTCCACGGAAACCGTATTGGAGACGGACACCTCCACGGAGGGACACGGATGACTGGAACCCTATTTGGGTGCCGTAAAGCTAGAGTTAACCTCGTTATCCAGGAAAGCCCTATATGTCTTCCCATGCTTGTCCTTGAGCTTGGGATACCCACAGGAAAGCTTCTCCAAGATATGGGAATGGGGCTTGTTAGAATAGCCCTGGAGTGCGAAAAGAAACCGGGCGAGAAGACGAAGATCGTTGATGAGCCGATATGGGCGTTGTATTGCAATGGGAAGAAATCAGGGTACGGAGTTAGAAGAGAACCGACGGGGGATGACTTGAGTGTGATGCAAGTGTTGCATGCAGCGTCCATGGGAGCTGGGGTGATACCGAATGAAACTGAGTTGCCAGATGGTGACTTAATGTACATGCGAGCACAGTTTGAACGTGTTGTTGGGTCTAGGGATTCGGAGACATATTATATGATGAATCCGGATGGAAACAATGGCCCTGAGCTCAGTGTGTTCTTCGTCAGGGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF617

Protein Analysis

270

Amino Acids

29.5

Weight (kDa)

9.37

Isoelectric Point (pI)

45.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF617 PF04759 113 - 268 1.9e-66 Protein of unknown function, DUF617
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015467)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G06990
fragaria_vesca FvH4_7g03890
malus_domestica MD07G1043800.v1.1
prunus_persica Prupe.2G043700_v2.0.a1
pyrus_communis pycom02g23680 pycom07g03140
rosa_chinensis RchiOBHm_Chr1g0327341
rosa_laevigata RLG00000029997
rosa_multiflora Rmu_sc0003600.1_g000036
rosa_roxburghii Rroxscaffold_4G00322730
rosa_rugosa Rorug01G0066800
rosa_samantha Rh1AG083500 Rh1BG066400 Rh1CG081400 Rh1DG087800
rosa_wichuraiana Rw1G006560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 357
AccB7I CCANNNNNTGG 1 cut(s) 460
AccIII TCCGGA 1 cut(s) 760
AciI CCGC 3 cut(s) 77, 80, 98
AclWI GGATC 1 cut(s) 271
AfaI GTAC 2 cut(s) 575, 695
AfiI CCNNNNNNNGG 2 cut(s) 354, 460
AflIII ACRYGT 1 cut(s) 715
AgsI TTSAA 2 cut(s) 42, 713
AjnI CCWGG 2 cut(s) 387, 486
AjuI GAANNNNNNNTTGG 2 cut(s) 705, 737
AluBI AGCT 5 cut(s) 368, 427, 448, 647, 784
AluI AGCT 5 cut(s) 368, 427, 448, 647, 784
Alw21I GWGCWC 2 cut(s) 706, 786
Alw26I GTCTC 2 cut(s) 303, 734
AlwI GGATC 1 cut(s) 271
Ama87I CYCGRG 1 cut(s) 31
Aor13HI TCCGGA 1 cut(s) 760
AoxI GGCC 2 cut(s) 196, 775
ApeKI GCWGC 2 cut(s) 74, 632
AspLEI GCGC 1 cut(s) 228
AspS9I GGNCC 3 cut(s) 120, 197, 776
AsuC2I CCSGG 1 cut(s) 507
AsuHPI GGTGA 4 cut(s) 105, 163, 664, 695
AvaI CYCGRG 1 cut(s) 31
AvaII GGWCC 1 cut(s) 120
BanI GGYRCC 1 cut(s) 357
BanII GRGCYC 1 cut(s) 786
BbsI GAAGAC 2 cut(s) 398, 521
Bbv12I GWGCWC 2 cut(s) 706, 786
BbvI GCAGC 2 cut(s) 86, 644
BccI CCATC 4 cut(s) 190, 208, 674, 758
BceAI ACGGC 2 cut(s) 11, 345
BciT130I CCWGG 2 cut(s) 389, 488
BciVI GTATCC 1 cut(s) 426
BcnI CCSGG 1 cut(s) 507
BcoDI GTCTC 2 cut(s) 303, 734
BfaI CTAG 2 cut(s) 369, 729
BfoI RGCGCY 1 cut(s) 229
BfuI GTATCC 1 cut(s) 426
BglII AGATCT 1 cut(s) 206
BisI GCNGC 3 cut(s) 75, 78, 633
BlsI GCNGC 3 cut(s) 76, 79, 634
Bme1390I CCNGG 3 cut(s) 389, 488, 507
Bme18I GGWCC 1 cut(s) 120
BmeT110I CYCGRG 1 cut(s) 31
BmgT120I GGNCC 3 cut(s) 120, 197, 776
BmiI GGNNCC 3 cut(s) 122, 346, 359
BmrFI CCNGG 3 cut(s) 389, 488, 507
BmsI GCATC 1 cut(s) 606
BpiI GAAGAC 2 cut(s) 398, 521
BplI GAGNNNNNCTC 2 cut(s) 363, 395
BpmI CTGGAG 1 cut(s) 509
Bpu10I CCTNAGC 1 cut(s) 780
BpuEI CTTGAG 2 cut(s) 443, 628
BpuMI CCSGG 1 cut(s) 507
BsaJI CCNNGG 5 cut(s) 116, 292, 322, 486, 639
BsaWI WCCGGW 2 cut(s) 243, 760
BsaXI ACNNNNNCTCC 2 cut(s) 636, 666
Bsc4I CCNNNNNNNGG 2 cut(s) 354, 460
Bse1I ACTGG 2 cut(s) 59, 346
Bse3DI GCAATG 2 cut(s) 21, 562
BseAI TCCGGA 1 cut(s) 760
BseBI CCWGG 2 cut(s) 389, 488
BseDI CCNNGG 5 cut(s) 116, 292, 322, 486, 639
BseGI GGATG 3 cut(s) 342, 607, 769
BseLI CCNNNNNNNGG 2 cut(s) 354, 460
BseMI GCAATG 2 cut(s) 21, 562
BseMII CTCAG 3 cut(s) 660, 771, 799
BseNI ACTGG 2 cut(s) 59, 346
BseXI GCAGC 2 cut(s) 86, 644
BseYI CCCAGC 1 cut(s) 647
BshFI GGCC 2 cut(s) 198, 777
BshNI GGYRCC 1 cut(s) 357
BsiHKAI GWGCWC 2 cut(s) 706, 786
BsiHKCI CYCGRG 1 cut(s) 31
BsiSI CCGG 3 cut(s) 244, 506, 761
BslFI GGGAC 3 cut(s) 14, 106, 343
BslI CCNNNNNNNGG 2 cut(s) 354, 460
BsmAI GTCTC 2 cut(s) 303, 734
BsmBI CGTCTC 1 cut(s) 303
BsmFI GGGAC 3 cut(s) 14, 106, 343
BsnI GGCC 2 cut(s) 198, 777
BsoBI CYCGRG 1 cut(s) 31
Bsp1286I GDGCHC 2 cut(s) 706, 786
Bsp13I TCCGGA 1 cut(s) 760
Bsp1407I TGTACA 1 cut(s) 693
Bsp143I GATC 3 cut(s) 206, 276, 522
Bsp19I CCATGG 2 cut(s) 116, 639
BspACI CCGC 3 cut(s) 77, 80, 98
BspANI GGCC 2 cut(s) 198, 777
BspCNI CTCAG 3 cut(s) 661, 772, 798
BspEI TCCGGA 1 cut(s) 760
BspLI GGNNCC 3 cut(s) 122, 346, 359
BspPI GGATC 1 cut(s) 271
BspT107I GGYRCC 1 cut(s) 357
BsrDI GCAATG 2 cut(s) 21, 562
BsrGI TGTACA 1 cut(s) 693
BsrI ACTGG 2 cut(s) 59, 346
BssECI CCNNGG 5 cut(s) 116, 292, 322, 486, 639
BssMI GATC 3 cut(s) 206, 276, 522
BssT1I CCWWGG 2 cut(s) 116, 639
Bst2UI CCWGG 2 cut(s) 389, 488
Bst4CI ACNGT 3 cut(s) 151, 302, 708
Bst6I CTCTTC 2 cut(s) 224, 580
BstAPI GCANNNNNTGC 1 cut(s) 625
BstAUI TGTACA 1 cut(s) 693
BstC8I GCNNGC 2 cut(s) 630, 702
BstDEI CTNAG 3 cut(s) 669, 780, 785
BstDSI CCRYGG 4 cut(s) 116, 292, 322, 639
BstF5I GGATG 3 cut(s) 342, 607, 769
BstH2I RGCGCY 1 cut(s) 229
BstHHI GCGC 1 cut(s) 228
BstKTI GATC 3 cut(s) 209, 279, 525
BstMAI GTCTC 2 cut(s) 303, 734
BstMBI GATC 3 cut(s) 206, 276, 522
BstMWI GCNNNNNNNGC 1 cut(s) 625
BstNI CCWGG 2 cut(s) 389, 488
BstNSI RCATGY 2 cut(s) 632, 700
BstSCI CCNGG 3 cut(s) 387, 486, 505
BstV1I GCAGC 2 cut(s) 86, 644
BstV2I GAAGAC 2 cut(s) 398, 521
BstX2I RGATCY 2 cut(s) 206, 276
BstYI RGATCY 2 cut(s) 206, 276
BsuI GTATCC 1 cut(s) 426
BsuRI GGCC 2 cut(s) 198, 777
BtgI CCRYGG 4 cut(s) 116, 292, 322, 639
BtsCI GGATG 3 cut(s) 342, 607, 769
BtsIMutI CAGTG 1 cut(s) 793
Cac8I GCNNGC 2 cut(s) 630, 702
CfoI GCGC 1 cut(s) 228
Cfr13I GGNCC 3 cut(s) 120, 197, 776
CseI GACGC 2 cut(s) 96, 624
Csp6I GTAC 2 cut(s) 574, 694
CviAII CATG 6 cut(s) 117, 270, 412, 629, 640, 697
CviQI GTAC 2 cut(s) 574, 694
DdeI CTNAG 3 cut(s) 669, 780, 785
DpnI GATC 3 cut(s) 208, 278, 524
DpnII GATC 3 cut(s) 206, 276, 522
Eam1104I CTCTTC 2 cut(s) 224, 580
EarI CTCTTC 2 cut(s) 224, 580
EciI GGCGGA 1 cut(s) 95
Ecl136II GAGCTC 1 cut(s) 784
Eco130I CCWWGG 2 cut(s) 116, 639
Eco24I GRGCYC 1 cut(s) 786
Eco47I GGWCC 1 cut(s) 120
Eco53kI GAGCTC 1 cut(s) 784
Eco88I CYCGRG 1 cut(s) 31
EcoICRI GAGCTC 1 cut(s) 784
EcoRII CCWGG 2 cut(s) 387, 486
EcoT14I CCWWGG 2 cut(s) 116, 639
EcoT38I GRGCYC 1 cut(s) 786
ErhI CCWWGG 2 cut(s) 116, 639
Esp3I CGTCTC 1 cut(s) 303
FaeI CATG 6 cut(s) 120, 273, 415, 632, 643, 700
FaqI GGGAC 3 cut(s) 14, 106, 343
FatI CATG 6 cut(s) 116, 269, 411, 628, 639, 696
Fnu4HI GCNGC 3 cut(s) 75, 78, 633
FokI GGATG 3 cut(s) 349, 614, 776
FriOI GRGCYC 1 cut(s) 786
Fsp4HI GCNGC 3 cut(s) 75, 78, 633
FspBI CTAG 2 cut(s) 369, 729
GlaI GCGC 1 cut(s) 227
GluI GCNGC 3 cut(s) 75, 78, 633
GsaI CCCAGC 1 cut(s) 651
GsuI CTGGAG 1 cut(s) 509
HaeII RGCGCY 1 cut(s) 229
HaeIII GGCC 2 cut(s) 198, 777
HapII CCGG 3 cut(s) 244, 506, 761
HgaI GACGC 2 cut(s) 96, 624
HhaI GCGC 1 cut(s) 228
Hin1II CATG 6 cut(s) 120, 273, 415, 632, 643, 700
Hin6I GCGC 1 cut(s) 226
HinP1I GCGC 1 cut(s) 226
HincII GTYRAC 1 cut(s) 376
HindII GTYRAC 1 cut(s) 376
HindIII AAGCTT 1 cut(s) 446
HinfI GANTC 2 cut(s) 734, 757
HpaI GTTAAC 1 cut(s) 376
HpaII CCGG 3 cut(s) 244, 506, 761
HphI GGTGA 4 cut(s) 105, 163, 664, 695
Hpy166II GTNNAC 1 cut(s) 376
Hpy188I TCNGA 1 cut(s) 739
Hpy188III TCNNGA 3 cut(s) 31, 204, 761
Hpy8I GTNNAC 1 cut(s) 376
Hpy99I CGWCG 2 cut(s) 112, 598
HpyAV CCTTC 1 cut(s) 276
HpyCH4III ACNGT 3 cut(s) 151, 302, 708
HpyCH4IV ACGT 1 cut(s) 715
HpyCH4V TGCA 5 cut(s) 273, 555, 619, 628, 632
HpyF10VI GCNNNNNNNGC 1 cut(s) 625
HpyF3I CTNAG 3 cut(s) 669, 780, 785
HpySE526I ACGT 1 cut(s) 715
Hsp92II CATG 6 cut(s) 120, 273, 415, 632, 643, 700
HspAI GCGC 1 cut(s) 226
Kpn2I TCCGGA 1 cut(s) 760
KspAI GTTAAC 1 cut(s) 376
Kzo9I GATC 3 cut(s) 206, 276, 522
LmnI GCTCC 2 cut(s) 294, 644
Lsp1109I GCAGC 2 cut(s) 86, 644
LweI GCATC 1 cut(s) 606
MaeI CTAG 2 cut(s) 369, 729
MaeII ACGT 1 cut(s) 715
MaeIII GTNAC 2 cut(s) 111, 683
MalI GATC 3 cut(s) 208, 278, 524
MboI GATC 3 cut(s) 206, 276, 522
MboII GAAGA 7 cut(s) 211, 398, 526, 532, 574, 597, 787
MflI RGATCY 2 cut(s) 206, 276
MhlI GDGCHC 2 cut(s) 706, 786
MluCI AATT 1 cut(s) 37
MmeI TCCRAC 1 cut(s) 202
MnlI CCTC 5 cut(s) 171, 185, 320, 329, 389
MroI TCCGGA 1 cut(s) 760
MseI TTAA 2 cut(s) 375, 689
MspA1I CMGCKG 1 cut(s) 77
MspI CCGG 3 cut(s) 244, 506, 761
MspR9I CCNGG 3 cut(s) 389, 488, 507
MvaI CCWGG 2 cut(s) 389, 488
MwoI GCNNNNNNNGC 1 cut(s) 625
NciI CCSGG 1 cut(s) 507
NcoI CCATGG 2 cut(s) 116, 639
NdeII GATC 3 cut(s) 206, 276, 522
NlaIII CATG 6 cut(s) 120, 273, 415, 632, 643, 700
NlaIV GGNNCC 3 cut(s) 122, 346, 359
NmuCI GTSAC 2 cut(s) 111, 683
NspI RCATGY 2 cut(s) 632, 700
PaeI GCATGC 1 cut(s) 632
PfeI GAWTC 2 cut(s) 734, 757
PflMI CCANNNNNTGG 1 cut(s) 460
PfoI TCCNGGA 1 cut(s) 387
PkrI GCNGC 3 cut(s) 76, 79, 634
Psp124BI GAGCTC 1 cut(s) 786
Psp6I CCWGG 2 cut(s) 387, 486
PspFI CCCAGC 1 cut(s) 647
PspGI CCWGG 2 cut(s) 387, 486
PspN4I GGNNCC 3 cut(s) 122, 346, 359
PspPI GGNCC 3 cut(s) 120, 197, 776
PsuI RGATCY 2 cut(s) 206, 276
RsaI GTAC 2 cut(s) 575, 695
RsaNI GTAC 2 cut(s) 574, 694
SacI GAGCTC 1 cut(s) 786
SaqAI TTAA 2 cut(s) 375, 689
SatI GCNGC 3 cut(s) 75, 78, 633
Sau3AI GATC 3 cut(s) 206, 276, 522
Sau96I GGNCC 3 cut(s) 120, 197, 776
ScrFI CCNGG 3 cut(s) 389, 488, 507
SduI GDGCHC 2 cut(s) 706, 786
SetI ASST 9 cut(s) 163, 321, 370, 381, 429, 450, 649, 718, 786
SfaNI GCATC 1 cut(s) 606
SinI GGWCC 1 cut(s) 120
SmlI CTYRAG 3 cut(s) 16, 422, 607
SmoI CTYRAG 3 cut(s) 16, 422, 607
SphI GCATGC 1 cut(s) 632
Sse9I AATT 1 cut(s) 37
SsiI CCGC 3 cut(s) 77, 80, 98
SspMI CTAG 2 cut(s) 369, 729
SstI GAGCTC 1 cut(s) 786
StyD4I CCNGG 3 cut(s) 387, 486, 505
StyI CCWWGG 2 cut(s) 116, 639
TaaI ACNGT 3 cut(s) 151, 302, 708
TaiI ACGT 1 cut(s) 718
TaqI TCGA 1 cut(s) 185
TasI AATT 1 cut(s) 37
TatI WGTACW 1 cut(s) 693
TauI GCSGC 1 cut(s) 80
TfiI GAWTC 2 cut(s) 734, 757
Tru1I TTAA 2 cut(s) 375, 689
Tru9I TTAA 2 cut(s) 375, 689
TscAI CASTG 1 cut(s) 793
TseFI GTSAC 2 cut(s) 111, 683
TseI GCWGC 2 cut(s) 74, 632
Tsp45I GTSAC 2 cut(s) 111, 683
TspDTI ATGAA 4 cut(s) 17, 258, 678, 770
TspGWI ACGGA 5 cut(s) 309, 327, 339, 349, 591
TspRI CASTG 1 cut(s) 793
Van91I CCANNNNNTGG 1 cut(s) 460
VpaK11BI GGWCC 1 cut(s) 120
XceI RCATGY 2 cut(s) 632, 700
XspI CTAG 2 cut(s) 369, 729
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.